FvH4_5g04580

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013664
Physical Location & Seq
Reverse (-)
2699236 .. 2702074
2839 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g04580.t1

Sequence Viewer

Length: 1230 bp
ATGCCTCCCTTACAAATCTTCTTCTCCTTCTCACTCTTCTTCCTCTTCCTCCTCTTTCCTCAAAGAGCAAGGGCAGCTCCGCCCGACTGCTCCATATCAAGCTGCCCTAACGACCCACGGGTACCAGTCAAGTTCCCTTTCCGGCTAAGACGCATCCAGCTCTCAGTTTGCGGCTATCCAGGGTTCGACCTCTCATGCAACAACCGGACTCAACTCACCACCCTCACCCTCCCCGGCTCCGGAGCCTTCCTCGTCAAGCGCATCGATTACAGGTCTCAAACGCTCTGGATCGACGACCGTAACTCCTGCCTCCCCAAAAGGCTCCTGTCCCATCCTTTCAACCTCCACGACACTCCATTCAGTGTCGTTGCCGGTTCCAAGAACTACACCTTCCTCAACTGCTCTTCCTCTCAGTCAACATCGACGTTACCGGCCACCGCGACTAGTATTTCTTGTCTAAGTGATGAAGGGTACAAGGTTTATGCTGTTCCCACGAGTGCGTACTATGCCTCTGCACCGGCAGCGGCGCCTTCATCTACGGGGTCGACTCCGGAACTATGTTCGGTGATTTCGACTGCTTTGGTTCCGAGAGGAGTGACGTATGAGTGGGAAGTAAATATGGGTGGGGAGGTTAGCCGCGGCGGTGTAGCGCTGGGGTGGTCTCAGCCGGATTGTAGGGAGTGTGAAGCTACGAATAAAAGTTGTGGATTCGACACAGATACAAGTCAGATTATGTGCTACAAAAGCAAGAAAAGCCATGTTCTCCCAAGAAGTGCAAAATACGGCATAATCATAGGTGTGGGAATACCAGGGCTCTTATTTATCATTGGCATTGCAAATTACATCTACAGCAGGATTTTCGGTCGCCGCCACCAGCCGAGCACAGACGAACTCTCCACCTCGACTGCTCTGCAATCCGCCAACGTTGTTGTAGGCCTCGACGCCCCAACCATTGAGTCATACCCCAAGACTTTGCTGGGAGAGAGCCGGAGATTACCTAGGGCCAACGACAATACTTGCTCCATTTGTTTGGGTGAGTACCAACCCAAAGAAGCACTGAGGACCATACCGGAATGTAACCATTACTTCCATGCTAATTGCATAGACGAATGGCTCAAACTGAATTCCTCGTGCCCAGTTTGCCGGAAGTCACCCGAGGTGTCATCTCATGTCACCCCTTCTTCTTCAGTGTCTTCATCAACATCGTCGTCTCTAGCATCAGTACAATAG

Protein Analysis

410

Amino Acids

44.57

Weight (kDa)

8.58

Isoelectric Point (pI)

58.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 30 - 99 2e-15 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 180 - 249 3.3e-06 Wall-associated receptor kinase C-terminal
zf-RING_2 PF13639 338 - 382 1.5e-13 Ring finger domain
zf-RING_11 PF17123 340 - 368 3.5e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 340 - 381 1.2e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 340 - 382 2.3e-08 RING-H2 zinc finger domain
zf-C3HC4 PF00097 340 - 381 2.5e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 121
AccB1I GGYRCC 2 cut(s) 121, 526
AccI GTMKAC 1 cut(s) 545
AccII CGCG 2 cut(s) 440, 639
AccIII TCCGGA 2 cut(s) 239, 550
AciI CCGC 9 cut(s) 80, 171, 438, 524, 637, 639, 642, 868, 918
AclI AACGTT 1 cut(s) 924
AclWI GGATC 1 cut(s) 296
AcoI YGGCCR 1 cut(s) 432
AcsI RAATTY 1 cut(s) 1123
AcuI CTGAAG 1 cut(s) 1170
AcyI GRCGYC 2 cut(s) 527, 942
AfaI GTAC 5 cut(s) 123, 473, 503, 1040, 1224
AfeI AGCGCT 1 cut(s) 651
AfiI CCNNNNNNNGG 1 cut(s) 239
AgsI TTSAA 1 cut(s) 340
AhlI ACTAGT 1 cut(s) 443
AjnI CCWGG 2 cut(s) 178, 808
AluBI AGCT 4 cut(s) 77, 102, 160, 689
AluI AGCT 4 cut(s) 77, 102, 160, 689
Alw21I GWGCWC 1 cut(s) 884
Alw26I GTCTC 3 cut(s) 279, 666, 1215
AlwI GGATC 1 cut(s) 296
Ama87I CYCGRG 1 cut(s) 1154
Aor13HI TCCGGA 2 cut(s) 239, 550
Aor51HI AGCGCT 1 cut(s) 651
AoxI GGCC 3 cut(s) 432, 934, 1002
ApeKI GCWGC 3 cut(s) 74, 102, 521
ApoI RAATTY 1 cut(s) 1123
Asp718I GGTACC 1 cut(s) 121
AspA2I CCTAGG 1 cut(s) 998
AspLEI GCGC 3 cut(s) 261, 529, 652
AspS9I GGNCC 2 cut(s) 1002, 1062
AsuC2I CCSGG 1 cut(s) 234
AsuHPI GGTGA 6 cut(s) 208, 217, 577, 1046, 1143, 1165
AvaI CYCGRG 1 cut(s) 1154
AvaII GGWCC 1 cut(s) 1062
AvrII CCTAGG 1 cut(s) 998
BaeGI GKGCMC 1 cut(s) 1136
BanI GGYRCC 2 cut(s) 121, 526
BanII GRGCYC 1 cut(s) 816
BauI CACGAG 2 cut(s) 493, 1129
BbsI GAAGAC 1 cut(s) 1185
Bbv12I GWGCWC 1 cut(s) 884
BbvI GCAGC 3 cut(s) 86, 89, 533
BccI CCATC 1 cut(s) 339
BceAI ACGGC 1 cut(s) 799
BcgI CGANNNNNNTGC 4 cut(s) 841, 875, 892, 926
BciT130I CCWGG 2 cut(s) 180, 810
BcnI CCSGG 1 cut(s) 234
BcoDI GTCTC 3 cut(s) 279, 666, 1215
BcuI ACTAGT 1 cut(s) 443
BfaI CTAG 3 cut(s) 444, 999, 1214
BfmI CTRYAG 1 cut(s) 847
BfoI RGCGCY 2 cut(s) 530, 653
BisI GCNGC 8 cut(s) 75, 103, 172, 522, 525, 637, 640, 868
BlnI CCTAGG 1 cut(s) 998
BlsI GCNGC 8 cut(s) 76, 104, 173, 523, 526, 638, 641, 869
Bme1390I CCNGG 3 cut(s) 180, 234, 810
Bme18I GGWCC 1 cut(s) 1062
BmeT110I CYCGRG 1 cut(s) 1154
BmgT120I GGNCC 2 cut(s) 1002, 1062
BmiI GGNNCC 7 cut(s) 123, 238, 244, 323, 376, 528, 585
BmrFI CCNGG 3 cut(s) 180, 234, 810
BmrI ACTGGG 1 cut(s) 1130
BmsI GCATC 3 cut(s) 162, 270, 1226
BmuI ACTGGG 1 cut(s) 1130
BpiI GAAGAC 1 cut(s) 1185
BplI GAGNNNNNCTC 2 cut(s) 234, 266
BpuMI CCSGG 1 cut(s) 234
Bsa29I ATCGAT 1 cut(s) 264
BsaHI GRCGYC 2 cut(s) 527, 942
BsaI GGTCTC 2 cut(s) 279, 666
BsaJI CCNNGG 7 cut(s) 116, 179, 232, 637, 809, 998, 1155
BsaWI WCCGGW 4 cut(s) 204, 239, 550, 1069
BsaXI ACNNNNNCTCC 4 cut(s) 287, 317, 878, 908
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse118I RCCGGY 3 cut(s) 371, 430, 517
Bse1I ACTGG 2 cut(s) 125, 1136
Bse3DI GCAATG 1 cut(s) 831
BseAI TCCGGA 2 cut(s) 239, 550
BseBI CCWGG 2 cut(s) 180, 810
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 7 cut(s) 116, 179, 232, 637, 809, 998, 1155
BseGI GGATG 2 cut(s) 153, 331
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 4 cut(s) 177, 425, 677, 1049
BseNI ACTGG 2 cut(s) 125, 1136
BseRI GAGGAG 2 cut(s) 41, 606
BseSI GKGCMC 1 cut(s) 1136
BseXI GCAGC 3 cut(s) 86, 89, 533
BseYI CCCAGC 2 cut(s) 652, 976
BsgI GTGCAG 1 cut(s) 498
Bsh1236I CGCG 2 cut(s) 440, 639
Bsh1285I CGRYCG 2 cut(s) 298, 865
BshFI GGCC 3 cut(s) 434, 936, 1004
BshNI GGYRCC 2 cut(s) 121, 526
BshVI ATCGAT 1 cut(s) 264
BsiEI CGRYCG 2 cut(s) 298, 865
BsiHKAI GWGCWC 1 cut(s) 884
BsiHKCI CYCGRG 1 cut(s) 1154
BslFI GGGAC 1 cut(s) 313
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 3 cut(s) 279, 666, 1215
BsmBI CGTCTC 1 cut(s) 1215
BsmFI GGGAC 1 cut(s) 313
BsnI GGCC 3 cut(s) 434, 936, 1004
Bso31I GGTCTC 2 cut(s) 279, 666
BsoBI CYCGRG 1 cut(s) 1154
Bsp1286I GDGCHC 3 cut(s) 816, 884, 1136
Bsp13I TCCGGA 2 cut(s) 239, 550
Bsp143I GATC 1 cut(s) 288
BspACI CCGC 9 cut(s) 80, 171, 438, 524, 637, 639, 642, 868, 918
BspANI GGCC 3 cut(s) 434, 936, 1004
BspCNI CTCAG 4 cut(s) 176, 424, 676, 1050
BspDI ATCGAT 1 cut(s) 264
BspEI TCCGGA 2 cut(s) 239, 550
BspFNI CGCG 2 cut(s) 440, 639
BspLI GGNNCC 7 cut(s) 123, 238, 244, 323, 376, 528, 585
BspPI GGATC 1 cut(s) 296
BspQI GCTCTTC 1 cut(s) 409
BspT107I GGYRCC 2 cut(s) 121, 526
BspTNI GGTCTC 2 cut(s) 279, 666
BsrDI GCAATG 1 cut(s) 831
BsrFI RCCGGY 3 cut(s) 371, 430, 517
BsrI ACTGG 2 cut(s) 125, 1136
BssAI RCCGGY 3 cut(s) 371, 430, 517
BssECI CCNNGG 7 cut(s) 116, 179, 232, 637, 809, 998, 1155
BssMI GATC 1 cut(s) 288
BssNI GRCGYC 2 cut(s) 527, 942
BssSI CACGAG 2 cut(s) 493, 1129
BssT1I CCWWGG 1 cut(s) 998
Bst2BI CACGAG 2 cut(s) 493, 1129
Bst2UI CCWGG 2 cut(s) 180, 810
Bst4CI ACNGT 1 cut(s) 299
Bst6I CTCTTC 3 cut(s) 41, 50, 409
BstACI GRCGYC 2 cut(s) 527, 942
BstDEI CTNAG 6 cut(s) 146, 163, 411, 458, 663, 1058
BstDSI CCRYGG 2 cut(s) 116, 637
BstF5I GGATG 2 cut(s) 153, 331
BstFNI CGCG 2 cut(s) 440, 639
BstH2I RGCGCY 2 cut(s) 530, 653
BstHHI GCGC 3 cut(s) 261, 529, 652
BstKTI GATC 1 cut(s) 291
BstMAI GTCTC 3 cut(s) 279, 666, 1215
BstMBI GATC 1 cut(s) 288
BstMCI CGRYCG 2 cut(s) 298, 865
BstMWI GCNNNNNNNGC 6 cut(s) 74, 506, 521, 744, 753, 1140
BstNI CCWGG 2 cut(s) 180, 810
BstSCI CCNGG 3 cut(s) 178, 232, 808
BstSFI CTRYAG 1 cut(s) 847
BstSLI GKGCMC 1 cut(s) 1136
BstUI CGCG 2 cut(s) 440, 639
BstV1I GCAGC 3 cut(s) 86, 89, 533
BstV2I GAAGAC 1 cut(s) 1185
BstXI CCANNNNNNTGG 1 cut(s) 1030
Bsu15I ATCGAT 1 cut(s) 264
BsuRI GGCC 3 cut(s) 434, 936, 1004
BsuTUI ATCGAT 1 cut(s) 264
BtgI CCRYGG 2 cut(s) 116, 637
BtsCI GGATG 2 cut(s) 153, 331
BtsIMutI CAGTG 3 cut(s) 367, 1055, 1194
CfoI GCGC 3 cut(s) 261, 529, 652
Cfr10I RCCGGY 3 cut(s) 371, 430, 517
Cfr13I GGNCC 2 cut(s) 1002, 1062
Cfr42I CCGCGG 1 cut(s) 640
ClaI ATCGAT 1 cut(s) 264
CseI GACGC 2 cut(s) 159, 950
Csp6I GTAC 5 cut(s) 122, 472, 502, 1039, 1223
CviAII CATG 4 cut(s) 195, 758, 1091, 1169
CviQI GTAC 5 cut(s) 122, 472, 502, 1039, 1223
DdeI CTNAG 6 cut(s) 146, 163, 411, 458, 663, 1058
DinI GGCGCC 1 cut(s) 528
DpnI GATC 1 cut(s) 290
DpnII GATC 1 cut(s) 288
EaeI YGGCCR 1 cut(s) 432
Eam1104I CTCTTC 3 cut(s) 41, 50, 409
EarI CTCTTC 3 cut(s) 41, 50, 409
EciI GGCGGA 2 cut(s) 69, 907
Eco130I CCWWGG 1 cut(s) 998
Eco147I AGGCCT 1 cut(s) 936
Eco24I GRGCYC 1 cut(s) 816
Eco31I GGTCTC 2 cut(s) 279, 666
Eco47I GGWCC 1 cut(s) 1062
Eco47III AGCGCT 1 cut(s) 651
Eco57I CTGAAG 1 cut(s) 1170
Eco88I CYCGRG 1 cut(s) 1154
EcoRI GAATTC 1 cut(s) 1123
EcoRII CCWGG 2 cut(s) 178, 808
EcoT14I CCWWGG 1 cut(s) 998
EcoT38I GRGCYC 1 cut(s) 816
EgeI GGCGCC 1 cut(s) 528
EheI GGCGCC 1 cut(s) 528
ErhI CCWWGG 1 cut(s) 998
Esp3I CGTCTC 1 cut(s) 1215
FaeI CATG 4 cut(s) 198, 761, 1094, 1172
FaqI GGGAC 1 cut(s) 313
FatI CATG 4 cut(s) 194, 757, 1090, 1168
FblI GTMKAC 1 cut(s) 545
Fnu4HI GCNGC 8 cut(s) 75, 103, 172, 522, 525, 637, 640, 868
FokI GGATG 2 cut(s) 140, 318
FriOI GRGCYC 1 cut(s) 816
Fsp4HI GCNGC 8 cut(s) 75, 103, 172, 522, 525, 637, 640, 868
FspBI CTAG 3 cut(s) 444, 999, 1214
GlaI GCGC 3 cut(s) 260, 528, 651
GluI GCNGC 8 cut(s) 75, 103, 172, 522, 525, 637, 640, 868
GsaI CCCAGC 2 cut(s) 656, 980
HaeII RGCGCY 2 cut(s) 530, 653
HaeIII GGCC 3 cut(s) 434, 936, 1004
HgaI GACGC 2 cut(s) 159, 950
HhaI GCGC 3 cut(s) 261, 529, 652
Hin1I GRCGYC 2 cut(s) 527, 942
Hin1II CATG 4 cut(s) 198, 761, 1094, 1172
Hin6I GCGC 3 cut(s) 259, 527, 650
HinP1I GCGC 3 cut(s) 259, 527, 650
HincII GTYRAC 2 cut(s) 417, 546
HindII GTYRAC 2 cut(s) 417, 546
HinfI GANTC 4 cut(s) 208, 547, 708, 956
HphI GGTGA 6 cut(s) 208, 217, 577, 1046, 1143, 1165
Hpy166II GTNNAC 2 cut(s) 417, 546
Hpy188I TCNGA 2 cut(s) 588, 729
Hpy188III TCNNGA 3 cut(s) 240, 286, 551
Hpy8I GTNNAC 2 cut(s) 417, 546
Hpy99I CGWCG 4 cut(s) 296, 427, 944, 1210
HpyAV CCTTC 6 cut(s) 37, 256, 400, 461, 540, 1188
HpyCH4III ACNGT 1 cut(s) 299
HpyCH4IV ACGT 3 cut(s) 425, 599, 924
HpyCH4V TGCA 6 cut(s) 198, 515, 776, 836, 913, 1101
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 506, 521, 744, 753, 1140
HpyF3I CTNAG 6 cut(s) 146, 163, 411, 458, 663, 1058
HpySE526I ACGT 3 cut(s) 425, 599, 924
Hsp92I GRCGYC 2 cut(s) 527, 942
Hsp92II CATG 4 cut(s) 198, 761, 1094, 1172
HspAI GCGC 3 cut(s) 259, 527, 650
KasI GGCGCC 1 cut(s) 526
Kpn2I TCCGGA 2 cut(s) 239, 550
KpnI GGTACC 1 cut(s) 125
KspI CCGCGG 1 cut(s) 640
Kzo9I GATC 1 cut(s) 288
LguI GCTCTTC 1 cut(s) 409
LmnI GCTCC 6 cut(s) 82, 95, 242, 242, 327, 1025
Lsp1109I GCAGC 3 cut(s) 86, 89, 533
LweI GCATC 3 cut(s) 162, 270, 1226
MaeI CTAG 3 cut(s) 444, 999, 1214
MaeII ACGT 3 cut(s) 425, 599, 924
MaeIII GTNAC 6 cut(s) 299, 426, 595, 1076, 1149, 1171
MalI GATC 1 cut(s) 290
MboI GATC 1 cut(s) 288
MboII GAAGA 9 cut(s) 10, 13, 28, 31, 37, 396, 1173, 1176, 1185
MhlI GDGCHC 3 cut(s) 816, 884, 1136
MluCI AATT 3 cut(s) 838, 1096, 1123
Mly113I GGCGCC 1 cut(s) 527
MlyI GAGTC 3 cut(s) 202, 541, 965
MroI TCCGGA 2 cut(s) 239, 550
MslI CAYNNNNRTG 1 cut(s) 797
MspA1I CMGCKG 2 cut(s) 524, 639
MspR9I CCNGG 3 cut(s) 180, 234, 810
MvaI CCWGG 2 cut(s) 180, 810
MvnI CGCG 2 cut(s) 440, 639
MwoI GCNNNNNNNGC 6 cut(s) 74, 506, 521, 744, 753, 1140
NarI GGCGCC 1 cut(s) 527
NciI CCSGG 1 cut(s) 234
NdeII GATC 1 cut(s) 288
NlaIII CATG 4 cut(s) 198, 761, 1094, 1172
NlaIV GGNNCC 7 cut(s) 123, 238, 244, 323, 376, 528, 585
NmeAIII GCCGAG 1 cut(s) 903
NmuCI GTSAC 3 cut(s) 595, 1149, 1171
PceI AGGCCT 1 cut(s) 936
PciSI GCTCTTC 1 cut(s) 409
PcsI WCGNNNNNNNCGW 1 cut(s) 569
PfeI GAWTC 1 cut(s) 708
PkrI GCNGC 8 cut(s) 76, 104, 173, 523, 526, 638, 641, 869
PleI GAGTC 3 cut(s) 202, 541, 964
PluTI GGCGCC 1 cut(s) 530
PpsI GAGTC 3 cut(s) 202, 541, 964
Psp1406I AACGTT 1 cut(s) 924
Psp6I CCWGG 2 cut(s) 178, 808
PspFI CCCAGC 2 cut(s) 652, 976
PspGI CCWGG 2 cut(s) 178, 808
PspN4I GGNNCC 7 cut(s) 123, 238, 244, 323, 376, 528, 585
PspPI GGNCC 2 cut(s) 1002, 1062
RsaI GTAC 5 cut(s) 123, 473, 503, 1040, 1224
RsaNI GTAC 5 cut(s) 122, 472, 502, 1039, 1223
RseI CAYNNNNRTG 1 cut(s) 797
SacII CCGCGG 1 cut(s) 640
SalI GTCGAC 1 cut(s) 544
SapI GCTCTTC 1 cut(s) 409
SatI GCNGC 8 cut(s) 75, 103, 172, 522, 525, 637, 640, 868
Sau3AI GATC 1 cut(s) 288
Sau96I GGNCC 2 cut(s) 1002, 1062
SchI GAGTC 3 cut(s) 202, 541, 965
ScrFI CCNGG 3 cut(s) 180, 234, 810
SduI GDGCHC 3 cut(s) 816, 884, 1136
SfaNI GCATC 3 cut(s) 162, 270, 1226
SfcI CTRYAG 1 cut(s) 847
SfoI GGCGCC 1 cut(s) 528
Sfr303I CCGCGG 1 cut(s) 640
SgrBI CCGCGG 1 cut(s) 640
SinI GGWCC 1 cut(s) 1062
SmiMI CAYNNNNRTG 1 cut(s) 797
SpeI ACTAGT 1 cut(s) 443
Sse9I AATT 3 cut(s) 838, 1096, 1123
SseBI AGGCCT 1 cut(s) 936
SsiI CCGC 9 cut(s) 80, 171, 438, 524, 637, 639, 642, 868, 918
SspDI GGCGCC 1 cut(s) 526
SspMI CTAG 3 cut(s) 444, 999, 1214
StuI AGGCCT 1 cut(s) 936
StyD4I CCNGG 3 cut(s) 178, 232, 808
StyI CCWWGG 1 cut(s) 998
TaaI ACNGT 1 cut(s) 299
TaiI ACGT 3 cut(s) 428, 602, 927
TaqI TCGA 9 cut(s) 186, 264, 291, 422, 545, 572, 711, 902, 939
TaqII GACCGA 1 cut(s) 851
TasI AATT 3 cut(s) 838, 1096, 1123
TatI WGTACW 1 cut(s) 1222
TauI GCSGC 5 cut(s) 174, 527, 639, 642, 870
TfiI GAWTC 1 cut(s) 708
TscAI CASTG 3 cut(s) 367, 1062, 1194
TseFI GTSAC 3 cut(s) 595, 1149, 1171
TseI GCWGC 3 cut(s) 74, 102, 521
Tsp45I GTSAC 3 cut(s) 595, 1149, 1171
TspDTI ATGAA 3 cut(s) 480, 522, 1185
TspRI CASTG 3 cut(s) 367, 1062, 1194
VpaK11BI GGWCC 1 cut(s) 1062
XapI RAATTY 1 cut(s) 1123
XcmI CCANNNNNNNNNTGG 1 cut(s) 973
XmaJI CCTAGG 1 cut(s) 998
XmiI GTMKAC 1 cut(s) 545
XspI CTAG 3 cut(s) 444, 999, 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.