Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0013664

Physical Location & Seq

Genomic Coordinates Reverse (-)

24628556 .. 24631385

Gene Structure 2830 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1106800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1236 bp

ATGGCTACCGTACAACTCTTCTCATTATTCTTACTTTTCTTGATCTTCTTCGTCCATCTAACAAGGGTTTCTTCCATCTGCCCTACATCAAACTGCTCCGACGGACCGCCGGTTCGATTCCCTTTCCGGCTTCGGAACCGCCAACCCTCACGATGTGGGTACAATCCAAGTTTTGACCTCTCATGCAGCAACCAAACCCTGATCACAACACTCACCCTCCCAGCCGCTACCGACAACTTCATAGTTCAACTCATCGACTACGAGTTTCAAACGCTTTACATCAACGACCCCAACCGCTGCCTCCCCAAACGGTTCCTAGACGACGTCTTCAAGCTCCAAGGCACGCCGTTTCACATCGAAATTTTCAAGAACCTCACCTTCCTCAACTGCTCCTCTCAGACACGAGCAAGCCAAGCGACGTCGTTACCCGTCATGGCCACTAGTATTTCATGTCTCAGCACTGAAAATCACACGGTCTTTGCTGTTCCATCGAGGGCTTATGAAGAATGGCTTCAGTCAACGTCATCTTCGTCACCGTCACCGTCACCGTCACCGTCAGAGGCTTTGTGTCCGGTGATTTCGAGTGTTTTGGTTCCGAGTACAGCTGCTAATGAGATGCTGGATATGTCTGGGGATGTTAGGAGTGGTGTTGGGTTGACTTGGTCAACGCCTGATTGTCGTGATTGTGAGGCCCGTGGTGGAGATTGTGGGTTTAACAGAGATGCGGATCGGGTTGTGTGCTCGAATATTCCTGAGGCTAAGAGGGAAGCTCTTCCAAGAAGTGCAAAATATGGCATAATCGTAGGTGTGGGAATACCCGGACTCTTATGCATCATTGGACTCGCAAGTTATGTCTTCGGCAGGATAAAGGCTTGCAGACGTCGCCACCAACCAAGTGTGGACCTCTCTACCTTAGCTTCTCGGCAACCTGACTTCGTCACAATAGGGCTTGATGGCCCAACCATTCAATCATACCCGAAGACATTTCTCGGGGATAGCAAGAGATTGCCCAAGCCCAATGACAACACGTGCTCAATATGTCTAAGTGAGTACGAGGCCAAGGAAGCGTTGAGGACCATACCAGAGTGTAACCATTACTTCCATGCTAGTTGCATAGACGAATGGCTTAAAATGAATCCAACGTGCCCCGTTTGTCGAAATTCACCCGAGGGCTCATCTCTTGCTATCCCTTCTTCCTCAGTGTCTTCATCAACATTTTCTTTAGCATCACCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GUB_WAK_bind WAK_assoc zf-RING_2

Protein Analysis

412

Amino Acids

45.02

Weight (kDa)

6.95

Isoelectric Point (pI)

63.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	27 - 96	1.3e-14	Wall-associated receptor kinase galacturonan-binding
WAK_assoc	PF14380	190 - 247	1.7e-07	Wall-associated receptor kinase C-terminal
zf-rbx1	PF12678	341 - 386	4.2e-09	RING-H2 zinc finger domain
zf-RING_2	PF13639	342 - 386	7.3e-15	Ring finger domain
zf-RING_11	PF17123	344 - 372	3.2e-09	RING-like zinc finger
zf-C3HC4_2	PF13923	344 - 385	5.5e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	344 - 385	5.5e-08	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013664)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g04580
malus_domestica	MD06G1106800.v1.1 MD14G1128800.v1.1
prunus_persica	Prupe.5G120000_v2.0.a1
pyrus_communis	pycom06g10100
rosa_chinensis	RchiOBHm_Chr7g0194801 RchiOBHm_Chr7g0194811
rosa_laevigata	RLG00000004167
rosa_multiflora	Rmu_co8285291.1_g000001 Rmu_sc0006960.1_g000001 Rmu_sc0036266.1_g000001
rosa_roxburghii	Rroxscaffold_3G00260970
rosa_rugosa	Rorug07G0018500.1
rosa_samantha	Rh7AG146200 Rh7BG146200 Rh7CG150100 Rh7DG147500
rosa_wichuraiana	Rw7G012480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	3 cut(s)	327, 422, 883
AciI	CCGC	5 cut(s)	107, 139, 225, 295, 725
AclWI	GGATC	1 cut(s)	735
AcoI	YGGCCR	1 cut(s)	435
AcsI	RAATTY	2 cut(s)	360, 1159
AcuI	CTGAAG	1 cut(s)	497
AcvI	CACGTG	1 cut(s)	1029
AcyI	GRCGYC	3 cut(s)	324, 419, 880
AdeI	CACNNNGTG	1 cut(s)	155
AfaI	GTAC	4 cut(s)	12, 161, 601, 1052
AflIII	ACRYGT	1 cut(s)	1026
AgsI	TTSAA	5 cut(s)	248, 269, 331, 367, 968
AhlI	ACTAGT	1 cut(s)	440
AloI	GAACNNNNNNTCC	2 cut(s)	96, 128
AluBI	AGCT	4 cut(s)	334, 605, 770, 917
AluI	AGCT	4 cut(s)	334, 605, 770, 917
Alw21I	GWGCWC	2 cut(s)	743, 1034
Alw26I	GTCTC	1 cut(s)	458
AlwI	GGATC	1 cut(s)	735
Ama87I	CYCGRG	2 cut(s)	989, 1166
AoxI	GGCC	4 cut(s)	435, 690, 955, 1056
ApeKI	GCWGC	3 cut(s)	186, 297, 605
ApoI	RAATTY	2 cut(s)	360, 1159
ArsI	GACNNNNNNTTYG	2 cut(s)	96, 128
Asp700I	GAANNNNTTC	2 cut(s)	510, 771
AspS9I	GGNCC	5 cut(s)	104, 691, 901, 956, 1074
AsuC2I	CCSGG	1 cut(s)	819
AsuHPI	GGTGA	9 cut(s)	205, 367, 525, 531, 537, 543, 586, 1155, 1221
AvaI	CYCGRG	2 cut(s)	989, 1166
AvaII	GGWCC	3 cut(s)	104, 901, 1074
AxyI	CCTNAGG	1 cut(s)	753
BaeGI	GKGCMC	1 cut(s)	1148
BalI	TGGCCA	1 cut(s)	437
BanII	GRGCYC	1 cut(s)	1175
BauI	CACGAG	1 cut(s)	402
BbrPI	CACGTG	1 cut(s)	1029
BbsI	GAAGAC	4 cut(s)	319, 847, 986, 1197
Bbv12I	GWGCWC	2 cut(s)	743, 1034
BbvI	GCAGC	3 cut(s)	198, 284, 592
BccI	CCATC	4 cut(s)	63, 83, 496, 947
BceAI	ACGGC	1 cut(s)	331
BclI	TGATCA	1 cut(s)	201
BcnI	CCSGG	1 cut(s)	819
BcoDI	GTCTC	1 cut(s)	458
BcuI	ACTAGT	1 cut(s)	440
BfaI	CTAG	3 cut(s)	317, 441, 1107
BisI	GCNGC	4 cut(s)	187, 225, 298, 606
BlsI	GCNGC	4 cut(s)	188, 226, 299, 607
Bme1390I	CCNGG	1 cut(s)	819
Bme18I	GGWCC	3 cut(s)	104, 901, 1074
BmeT110I	CYCGRG	2 cut(s)	989, 1166
BmgT120I	GGNCC	5 cut(s)	104, 691, 901, 956, 1074
BmiI	GGNNCC	3 cut(s)	137, 314, 594
BmrFI	CCNGG	1 cut(s)	819
BmsI	GCATC	3 cut(s)	606, 712, 840
BpiI	GAAGAC	4 cut(s)	319, 847, 986, 1197
BplI	GAGNNNNNCTC	2 cut(s)	754, 786
Bpu10I	CCTNAGC	1 cut(s)	913
BpuMI	CCSGG	1 cut(s)	819
BsaAI	YACGTR	1 cut(s)	1029
BsaBI	GATNNNNATC	1 cut(s)	726
BsaHI	GRCGYC	3 cut(s)	324, 419, 880
BsaJI	CCNNGG	4 cut(s)	337, 694, 1059, 1167
BsaWI	WCCGGW	1 cut(s)	571
BsaXI	ACNNNNNCTCC	2 cut(s)	201, 231
Bse118I	RCCGGY	1 cut(s)	109
Bse21I	CCTNAGG	1 cut(s)	753
Bse8I	GATNNNNATC	1 cut(s)	726
BseDI	CCNNGG	4 cut(s)	337, 694, 1059, 1167
BseGI	GGATG	1 cut(s)	640
BseJI	GATNNNNATC	1 cut(s)	726
BseMII	CTCAG	4 cut(s)	410, 469, 744, 1212
BseRI	GAGGAG	1 cut(s)	382
BseSI	GKGCMC	1 cut(s)	1148
BseXI	GCAGC	3 cut(s)	198, 284, 592
BseYI	CCCAGC	1 cut(s)	220
BshFI	GGCC	4 cut(s)	437, 692, 957, 1058
BsiHKAI	GWGCWC	2 cut(s)	743, 1034
BsiHKCI	CYCGRG	2 cut(s)	989, 1166
BsiSI	CCGG	4 cut(s)	110, 127, 572, 819
BsmAI	GTCTC	1 cut(s)	458
BsnI	GGCC	4 cut(s)	437, 692, 957, 1058
BsoBI	CYCGRG	2 cut(s)	989, 1166
Bsp1286I	GDGCHC	4 cut(s)	743, 1034, 1148, 1175
Bsp143I	GATC	3 cut(s)	42, 201, 727
BspACI	CCGC	5 cut(s)	107, 139, 225, 295, 725
BspANI	GGCC	4 cut(s)	437, 692, 957, 1058
BspCNI	CTCAG	4 cut(s)	409, 468, 745, 1211
BspLI	GGNNCC	3 cut(s)	137, 314, 594
BspPI	GGATC	1 cut(s)	735
BspQI	GCTCTTC	1 cut(s)	777
BsrFI	RCCGGY	1 cut(s)	109
BssAI	RCCGGY	1 cut(s)	109
BssECI	CCNNGG	4 cut(s)	337, 694, 1059, 1167
BssMI	GATC	3 cut(s)	42, 201, 727
BssNI	GRCGYC	3 cut(s)	324, 419, 880
BssSI	CACGAG	1 cut(s)	402
BssT1I	CCWWGG	2 cut(s)	337, 1059
Bst2BI	CACGAG	1 cut(s)	402
Bst4CI	ACNGT	7 cut(s)	10, 312, 475, 537, 543, 549, 555
Bst6I	CTCTTC	2 cut(s)	23, 777
BstACI	GRCGYC	3 cut(s)	324, 419, 880
BstBAI	YACGTR	1 cut(s)	1029
BstC8I	GCNNGC	3 cut(s)	344, 409, 874
BstDEI	CTNAG	7 cut(s)	396, 455, 753, 759, 913, 1043, 1198
BstDSI	CCRYGG	1 cut(s)	694
BstF5I	GGATG	1 cut(s)	640
BstKTI	GATC	3 cut(s)	45, 204, 730
BstMAI	GTCTC	1 cut(s)	458
BstMBI	GATC	3 cut(s)	42, 201, 727
BstMWI	GCNNNNNNNGC	3 cut(s)	413, 882, 1064
BstSCI	CCNGG	1 cut(s)	817
BstSLI	GKGCMC	1 cut(s)	1148
BstV1I	GCAGC	3 cut(s)	198, 284, 592
BstV2I	GAAGAC	4 cut(s)	319, 847, 986, 1197
Bsu36I	CCTNAGG	1 cut(s)	753
BsuRI	GGCC	4 cut(s)	437, 692, 957, 1058
BtgI	CCRYGG	1 cut(s)	694
BtsCI	GGATG	1 cut(s)	640
BtsIMutI	CAGTG	2 cut(s)	459, 1206
Cac8I	GCNNGC	3 cut(s)	344, 409, 874
Cfr10I	RCCGGY	1 cut(s)	109
Cfr13I	GGNCC	5 cut(s)	104, 691, 901, 956, 1074
CpoI	CGGWCCG	1 cut(s)	104
Csp6I	GTAC	4 cut(s)	11, 160, 600, 1051
CspI	CGGWCCG	1 cut(s)	104
CviAII	CATG	4 cut(s)	183, 433, 450, 1103
CviQI	GTAC	4 cut(s)	11, 160, 600, 1051
DdeI	CTNAG	7 cut(s)	396, 455, 753, 759, 913, 1043, 1198
DpnI	GATC	3 cut(s)	44, 203, 729
DpnII	GATC	3 cut(s)	42, 201, 727
DraIII	CACNNNGTG	1 cut(s)	155
EaeI	YGGCCR	1 cut(s)	435
Eam1104I	CTCTTC	2 cut(s)	23, 777
EarI	CTCTTC	2 cut(s)	23, 777
Eco130I	CCWWGG	2 cut(s)	337, 1059
Eco24I	GRGCYC	1 cut(s)	1175
Eco47I	GGWCC	3 cut(s)	104, 901, 1074
Eco57I	CTGAAG	1 cut(s)	497
Eco72I	CACGTG	1 cut(s)	1029
Eco81I	CCTNAGG	1 cut(s)	753
Eco88I	CYCGRG	2 cut(s)	989, 1166
EcoT14I	CCWWGG	2 cut(s)	337, 1059
EcoT22I	ATGCAT	1 cut(s)	833
EcoT38I	GRGCYC	1 cut(s)	1175
ErhI	CCWWGG	2 cut(s)	337, 1059
FaeI	CATG	4 cut(s)	186, 436, 453, 1106
FalI	AAGNNNNNCTT	4 cut(s)	55, 87, 495, 527
FatI	CATG	4 cut(s)	182, 432, 449, 1102
FbaI	TGATCA	1 cut(s)	201
Fnu4HI	GCNGC	4 cut(s)	187, 225, 298, 606
FokI	GGATG	1 cut(s)	647
FriOI	GRGCYC	1 cut(s)	1175
Fsp4HI	GCNGC	4 cut(s)	187, 225, 298, 606
FspBI	CTAG	3 cut(s)	317, 441, 1107
GluI	GCNGC	4 cut(s)	187, 225, 298, 606
GsaI	CCCAGC	1 cut(s)	224
HaeIII	GGCC	4 cut(s)	437, 692, 957, 1058
HapII	CCGG	4 cut(s)	110, 127, 572, 819
Hin1I	GRCGYC	3 cut(s)	324, 419, 880
Hin1II	CATG	4 cut(s)	186, 436, 453, 1106
HincII	GTYRAC	3 cut(s)	519, 657, 666
HindII	GTYRAC	3 cut(s)	519, 657, 666
HinfI	GANTC	4 cut(s)	117, 822, 840, 1135
HpaII	CCGG	4 cut(s)	110, 127, 572, 819
HphI	GGTGA	9 cut(s)	205, 367, 525, 531, 537, 543, 586, 1155, 1221
Hpy166II	GTNNAC	4 cut(s)	519, 657, 666, 901
Hpy188I	TCNGA	5 cut(s)	100, 135, 399, 559, 597
Hpy188III	TCNNGA	5 cut(s)	40, 150, 367, 680, 752
Hpy8I	GTNNAC	4 cut(s)	519, 657, 666, 901
Hpy99I	CGWCG	5 cut(s)	104, 326, 421, 424, 885
HpyAV	CCTTC	2 cut(s)	388, 1200
HpyCH4III	ACNGT	7 cut(s)	10, 312, 475, 537, 543, 549, 555
HpyCH4IV	ACGT	6 cut(s)	324, 419, 521, 880, 1028, 1142
HpyCH4V	TGCA	5 cut(s)	186, 785, 831, 876, 1113
HpyF10VI	GCNNNNNNNGC	3 cut(s)	413, 882, 1064
HpyF3I	CTNAG	7 cut(s)	396, 455, 753, 759, 913, 1043, 1198
HpySE526I	ACGT	6 cut(s)	324, 419, 521, 880, 1028, 1142
Hsp92I	GRCGYC	3 cut(s)	324, 419, 880
Hsp92II	CATG	4 cut(s)	186, 436, 453, 1106
Ksp22I	TGATCA	1 cut(s)	201
Kzo9I	GATC	3 cut(s)	42, 201, 727
LguI	GCTCTTC	1 cut(s)	777
LmnI	GCTCC	3 cut(s)	101, 339, 395
Lsp1109I	GCAGC	3 cut(s)	198, 284, 592
LweI	GCATC	3 cut(s)	606, 712, 840
MaeI	CTAG	3 cut(s)	317, 441, 1107
MaeII	ACGT	6 cut(s)	324, 419, 521, 880, 1028, 1142
MaeIII	GTNAC	7 cut(s)	423, 531, 537, 543, 549, 937, 1088
MalI	GATC	3 cut(s)	44, 203, 729
MboI	GATC	3 cut(s)	42, 201, 727
MhlI	GDGCHC	4 cut(s)	743, 1034, 1148, 1175
MlsI	TGGCCA	1 cut(s)	437
MluCI	AATT	2 cut(s)	360, 1159
MluNI	TGGCCA	1 cut(s)	437
MlyI	GAGTC	2 cut(s)	816, 834
MmeI	TCCRAC	2 cut(s)	123, 1163
Mox20I	TGGCCA	1 cut(s)	437
Mph1103I	ATGCAT	1 cut(s)	833
MroXI	GAANNNNTTC	2 cut(s)	510, 771
MscI	TGGCCA	1 cut(s)	437
MseI	TTAA	2 cut(s)	714, 1128
Msp20I	TGGCCA	1 cut(s)	437
MspA1I	CMGCKG	2 cut(s)	297, 605
MspI	CCGG	4 cut(s)	110, 127, 572, 819
MspR9I	CCNGG	1 cut(s)	819
MwoI	GCNNNNNNNGC	3 cut(s)	413, 882, 1064
NciI	CCSGG	1 cut(s)	819
NdeII	GATC	3 cut(s)	42, 201, 727
NlaIII	CATG	4 cut(s)	186, 436, 453, 1106
NlaIV	GGNNCC	3 cut(s)	137, 314, 594
NmeAIII	GCCGAG	1 cut(s)	901
NmuCI	GTSAC	5 cut(s)	531, 537, 543, 549, 937
NsiI	ATGCAT	1 cut(s)	833
PciSI	GCTCTTC	1 cut(s)	777
PcsI	WCGNNNNNNNCGW	1 cut(s)	527
PdmI	GAANNNNTTC	2 cut(s)	510, 771
PfeI	GAWTC	2 cut(s)	117, 1135
PflFI	GACNNNGTC	3 cut(s)	323, 661, 935
PkrI	GCNGC	4 cut(s)	188, 226, 299, 607
PleI	GAGTC	2 cut(s)	816, 834
PmaCI	CACGTG	1 cut(s)	1029
PmlI	CACGTG	1 cut(s)	1029
PpsI	GAGTC	2 cut(s)	816, 834
Ppu21I	YACGTR	1 cut(s)	1029
PspCI	CACGTG	1 cut(s)	1029
PspFI	CCCAGC	1 cut(s)	220
PspN4I	GGNNCC	3 cut(s)	137, 314, 594
PspPI	GGNCC	5 cut(s)	104, 691, 901, 956, 1074
PsyI	GACNNNGTC	3 cut(s)	323, 661, 935
PvuII	CAGCTG	1 cut(s)	605
RsaI	GTAC	4 cut(s)	12, 161, 601, 1052
RsaNI	GTAC	4 cut(s)	11, 160, 600, 1051
Rsr2I	CGGWCCG	1 cut(s)	104
RsrII	CGGWCCG	1 cut(s)	104
SapI	GCTCTTC	1 cut(s)	777
SaqAI	TTAA	2 cut(s)	714, 1128
SatI	GCNGC	4 cut(s)	187, 225, 298, 606
Sau3AI	GATC	3 cut(s)	42, 201, 727
Sau96I	GGNCC	5 cut(s)	104, 691, 901, 956, 1074
SchI	GAGTC	2 cut(s)	816, 834
ScrFI	CCNGG	1 cut(s)	819
SduI	GDGCHC	4 cut(s)	743, 1034, 1148, 1175
SfaNI	GCATC	3 cut(s)	606, 712, 840
SinI	GGWCC	3 cut(s)	104, 901, 1074
SpeI	ACTAGT	1 cut(s)	440
Sse9I	AATT	2 cut(s)	360, 1159
SsiI	CCGC	5 cut(s)	107, 139, 225, 295, 725
SspI	AATATT	1 cut(s)	748
SspMI	CTAG	3 cut(s)	317, 441, 1107
StyD4I	CCNGG	1 cut(s)	817
StyI	CCWWGG	2 cut(s)	337, 1059
TaaI	ACNGT	7 cut(s)	10, 312, 475, 537, 543, 549, 555
TaiI	ACGT	6 cut(s)	327, 422, 524, 883, 1031, 1145
TaqI	TCGA	7 cut(s)	115, 255, 357, 491, 581, 743, 1156
TasI	AATT	2 cut(s)	360, 1159
TatI	WGTACW	1 cut(s)	599
TauI	GCSGC	1 cut(s)	227
TfiI	GAWTC	2 cut(s)	117, 1135
Tru1I	TTAA	2 cut(s)	714, 1128
Tru9I	TTAA	2 cut(s)	714, 1128
TscAI	CASTG	2 cut(s)	466, 1206
TseFI	GTSAC	5 cut(s)	531, 537, 543, 549, 937
TseI	GCWGC	3 cut(s)	186, 297, 605
Tsp45I	GTSAC	5 cut(s)	531, 537, 543, 549, 937
TspDTI	ATGAA	5 cut(s)	229, 438, 516, 1148, 1197
TspGWI	ACGGA	1 cut(s)	117
TspRI	CASTG	2 cut(s)	466, 1206
Tth111I	GACNNNGTC	3 cut(s)	323, 661, 935
VpaK11BI	GGWCC	3 cut(s)	104, 901, 1074
XapI	RAATTY	2 cut(s)	360, 1159
XmnI	GAANNNNTTC	2 cut(s)	510, 771
XspI	CTAG	3 cut(s)	317, 441, 1107
ZraI	GACGTC	3 cut(s)	325, 420, 881
Zsp2I	ATGCAT	1 cut(s)	833

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.