MD14G1128800.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013664
Physical Location & Seq
Reverse (-)
20575934 .. 20579478
3545 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1128800.v1.1.491

Sequence Viewer

Length: 1248 bp
ATGGCTGCCTTACAACTCTTCTCATTATTCTTACTTTTCGTGATCATCTTCTTCCATCCAACAAGGGCTTCTTCCATCTGCCCTGCATCAAACTGCCCCCGCGGACCTCCGGTCCGATTCCCTTTCTGGCTTCGAAACCTCCAATCCCCACGCTGTCGGTACAACAATCCAGGGTTTGACCTCTCATGCAGCAACCAAACCCCATTCATGACCACACTCACCCTCCCAGCCTCTTCCGACAACTTCATAGTCCAAAGCATCGACTACGACTTCCAAACGCTTTACATCAACGACCCCAACCACTGCCTCCCCAAACGGTTCCTAAACAACACCTTCAACCTCCACGGCACGCCGTTCCACTTCGACAGAGTCCAGAACCTCACCTTCCTCAACTGCTCCTCTCTGGCACGAGCAAGCCACGCGACGTCGATACCCTTCATAGCCACGATTATTTCCTGTCTCAGCACTGAGAATCACATGGTGTTTGCTGTTCGGTCGGGGGTGTACGAAAATTGGCTTCAGTCAACGTCGTCTTCGTCAGCTTCACCGTCACCGTCACCGTTACCGTCAGTGGTTTTGTGTCCGGTGATTTCGAGTGTTGTGGTTCCGACTACAGCTGCTGATGAGATGCTGGAGTTGTTTGGGGATGTTTGGCGTGGTGTTGGGTTGACTTGGTCAACGCCTGACTGTCCTGATTGTGAAGCCCGTGGTGGAGATTGTGGGTTTAACAGAGATGTTGATCGGATTGTGTGCTCGAATATTCCTGAGGCTAAGAGTGAAGGTCTTCCAAGAAGCGCAAAATATGGTATAATCATAGGTGTGGGAATACCCGGACTATTATGCATCATTGGACTCGCAAGTTATATCTTCGGAAGGATAAAGGCTCACGCTCATCGCCACCAGCCAAGCATGGATCTCTCCACCTTAGCTTCTCGGCAACCTACCTTCTTCACAATAGGGCTTGATGGCCCAACCATTCAATCATACCCCAAGACTTTGCTCGGAGAGAGTAAGAGATTGCCCAAGCCCAACGACAACATGTGTTCAATATGTTTAAGTGAGTACGAGGCCAAGGAGGTGTTGAGGACTATACCAGAGTGTAATCATTACTTCCATGCTAGTTGCATAGACGAATGGCTTAAAATGAATGCAACATGCCCCGTTTGTAGGAATTCACCAGAGGGCTCATCTGTTGCTACCCATTCTTTCTCAATGTCTTCTTCAACTTTTTCTTTAGCATCACCATAG

Protein Analysis

416

Amino Acids

45.66

Weight (kDa)

6.84

Isoelectric Point (pI)

54.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 27 - 98 2.7e-14 Wall-associated receptor kinase galacturonan-binding
zf-RING_2 PF13639 346 - 390 2e-14 Ring finger domain
zf-rbx1 PF12678 346 - 390 6.9e-10 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 347 - 389 7.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 348 - 376 1.5e-08 RING-like zinc finger
zf-C3HC4 PF00097 348 - 389 6.2e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 428
AccII CGCG 2 cut(s) 102, 422
AciI CCGC 2 cut(s) 100, 102
AclWI GGATC 1 cut(s) 921
AcsI RAATTY 1 cut(s) 1171
AcuI CTGAAG 1 cut(s) 503
AcyI GRCGYC 1 cut(s) 425
AdeI CACNNNGTG 1 cut(s) 481
AfaI GTAC 3 cut(s) 161, 506, 1064
AfiI CCNNNNNNNGG 1 cut(s) 1167
AflIII ACRYGT 1 cut(s) 1038
AgsI TTSAA 4 cut(s) 337, 980, 1047, 1224
AhdI GACNNNNNGTC 1 cut(s) 110
AjnI CCWGG 1 cut(s) 169
AjuI GAANNNNNNNTTGG 2 cut(s) 52, 84
AluBI AGCT 3 cut(s) 542, 617, 929
AluI AGCT 3 cut(s) 542, 617, 929
Alw21I GWGCWC 1 cut(s) 755
Alw26I GTCTC 1 cut(s) 464
AlwI GGATC 1 cut(s) 921
AlwNI CAGNNNCTG 1 cut(s) 620
AoxI GGCC 2 cut(s) 967, 1068
ApeKI GCWGC 3 cut(s) 5, 189, 617
ApoI RAATTY 1 cut(s) 1171
Asp700I GAANNNNTTC 1 cut(s) 783
AspLEI GCGC 1 cut(s) 797
AspS9I GGNCC 3 cut(s) 104, 112, 968
AsuC2I CCSGG 1 cut(s) 831
AsuHPI GGTGA 8 cut(s) 211, 373, 537, 543, 549, 598, 1167, 1233
AsuII TTCGAA 1 cut(s) 133
AvaII GGWCC 2 cut(s) 104, 112
AxyI CCTNAGG 1 cut(s) 765
BanII GRGCYC 1 cut(s) 1187
BauI CACGAG 1 cut(s) 408
BbsI GAAGAC 3 cut(s) 525, 776, 1209
Bbv12I GWGCWC 1 cut(s) 755
BbvI GCAGC 2 cut(s) 201, 604
BccI CCATC 3 cut(s) 63, 83, 959
BceAI ACGGC 2 cut(s) 337, 361
BciT130I CCWGG 1 cut(s) 171
BclI TGATCA 1 cut(s) 42
BcnI CCSGG 1 cut(s) 831
BcoDI GTCTC 1 cut(s) 464
BfaI CTAG 1 cut(s) 1119
BfmI CTRYAG 1 cut(s) 612
BisI GCNGC 3 cut(s) 6, 190, 618
BlsI GCNGC 3 cut(s) 7, 191, 619
Bme1390I CCNGG 2 cut(s) 171, 831
Bme18I GGWCC 2 cut(s) 104, 112
BmeRI GACNNNNNGTC 1 cut(s) 110
BmgT120I GGNCC 3 cut(s) 104, 112, 968
BmiI GGNNCC 2 cut(s) 320, 606
BmrFI CCNGG 2 cut(s) 171, 831
BmsI GCATC 4 cut(s) 95, 267, 618, 852
BpiI GAAGAC 3 cut(s) 525, 776, 1209
BpmI CTGGAG 1 cut(s) 653
Bpu10I CCTNAGC 1 cut(s) 925
Bpu14I TTCGAA 1 cut(s) 133
BpuMI CCSGG 1 cut(s) 831
BsaBI GATNNNNATC 1 cut(s) 738
BsaHI GRCGYC 1 cut(s) 425
BsaJI CCNNGG 5 cut(s) 100, 170, 343, 706, 1071
BsaWI WCCGGW 2 cut(s) 109, 583
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bsc4I CCNNNNNNNGG 1 cut(s) 1167
Bse21I CCTNAGG 1 cut(s) 765
Bse8I GATNNNNATC 1 cut(s) 738
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 5 cut(s) 100, 170, 343, 706, 1071
BseGI GGATG 2 cut(s) 55, 652
BseJI GATNNNNATC 1 cut(s) 738
BseLI CCNNNNNNNGG 1 cut(s) 1167
BseMII CTCAG 3 cut(s) 459, 475, 756
BseRI GAGGAG 1 cut(s) 388
BseXI GCAGC 2 cut(s) 201, 604
BseYI CCCAGC 1 cut(s) 226
Bsh1236I CGCG 2 cut(s) 102, 422
Bsh1285I CGRYCG 1 cut(s) 497
BshFI GGCC 2 cut(s) 969, 1070
BsiEI CGRYCG 1 cut(s) 497
BsiHKAI GWGCWC 1 cut(s) 755
BsiSI CCGG 3 cut(s) 110, 584, 831
BslI CCNNNNNNNGG 1 cut(s) 1167
BsmAI GTCTC 1 cut(s) 464
BsmI GAATGC 1 cut(s) 1153
BsnI GGCC 2 cut(s) 969, 1070
Bsp119I TTCGAA 1 cut(s) 133
Bsp1286I GDGCHC 2 cut(s) 755, 1187
Bsp143I GATC 3 cut(s) 42, 739, 913
BspACI CCGC 2 cut(s) 100, 102
BspANI GGCC 2 cut(s) 969, 1070
BspCNI CTCAG 3 cut(s) 460, 474, 757
BspFNI CGCG 2 cut(s) 102, 422
BspHI TCATGA 1 cut(s) 207
BspLI GGNNCC 2 cut(s) 320, 606
BspPI GGATC 1 cut(s) 921
BspT104I TTCGAA 1 cut(s) 133
BssECI CCNNGG 5 cut(s) 100, 170, 343, 706, 1071
BssMI GATC 3 cut(s) 42, 739, 913
BssNI GRCGYC 1 cut(s) 425
BssSI CACGAG 1 cut(s) 408
BssT1I CCWWGG 1 cut(s) 1071
Bst2BI CACGAG 1 cut(s) 408
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 6 cut(s) 318, 549, 555, 561, 567, 689
Bst6I CTCTTC 2 cut(s) 23, 238
BstACI GRCGYC 1 cut(s) 425
BstBI TTCGAA 1 cut(s) 133
BstC8I GCNNGC 2 cut(s) 350, 415
BstDEI CTNAG 5 cut(s) 461, 468, 765, 771, 925
BstDSI CCRYGG 3 cut(s) 100, 343, 706
BstF5I GGATG 2 cut(s) 55, 652
BstFNI CGCG 2 cut(s) 102, 422
BstHHI GCGC 1 cut(s) 797
BstKTI GATC 3 cut(s) 45, 742, 916
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 3 cut(s) 42, 739, 913
BstMCI CGRYCG 1 cut(s) 497
BstMWI GCNNNNNNNGC 1 cut(s) 419
BstNI CCWGG 1 cut(s) 171
BstNSI RCATGY 2 cut(s) 1042, 1158
BstSCI CCNGG 2 cut(s) 169, 829
BstSFI CTRYAG 1 cut(s) 612
BstUI CGCG 2 cut(s) 102, 422
BstV1I GCAGC 2 cut(s) 201, 604
BstV2I GAAGAC 3 cut(s) 525, 776, 1209
BstX2I RGATCY 1 cut(s) 913
BstYI RGATCY 1 cut(s) 913
Bsu36I CCTNAGG 1 cut(s) 765
BsuRI GGCC 2 cut(s) 969, 1070
BtgI CCRYGG 3 cut(s) 100, 343, 706
BtgZI GCGATG 1 cut(s) 878
BtsCI GGATG 2 cut(s) 55, 652
BtsI GCAGTG 1 cut(s) 301
BtsIMutI CAGTG 3 cut(s) 301, 465, 576
Cac8I GCNNGC 2 cut(s) 350, 415
CaiI CAGNNNCTG 1 cut(s) 620
CciI TCATGA 1 cut(s) 207
CfoI GCGC 1 cut(s) 797
Cfr13I GGNCC 3 cut(s) 104, 112, 968
Cfr42I CCGCGG 1 cut(s) 103
CpoI CGGWCCG 1 cut(s) 112
Csp6I GTAC 3 cut(s) 160, 505, 1063
CspI CGGWCCG 1 cut(s) 112
CviAII CATG 7 cut(s) 186, 208, 478, 910, 1039, 1115, 1155
CviQI GTAC 3 cut(s) 160, 505, 1063
DdeI CTNAG 5 cut(s) 461, 468, 765, 771, 925
DpnI GATC 3 cut(s) 44, 741, 915
DpnII GATC 3 cut(s) 42, 739, 913
DraIII CACNNNGTG 1 cut(s) 481
DriI GACNNNNNGTC 1 cut(s) 110
Eam1104I CTCTTC 2 cut(s) 23, 238
Eam1105I GACNNNNNGTC 1 cut(s) 110
EarI CTCTTC 2 cut(s) 23, 238
Eco130I CCWWGG 1 cut(s) 1071
Eco24I GRGCYC 1 cut(s) 1187
Eco47I GGWCC 2 cut(s) 104, 112
Eco57I CTGAAG 1 cut(s) 503
Eco81I CCTNAGG 1 cut(s) 765
EcoRI GAATTC 1 cut(s) 1171
EcoRII CCWGG 1 cut(s) 169
EcoT14I CCWWGG 1 cut(s) 1071
EcoT22I ATGCAT 1 cut(s) 845
EcoT38I GRGCYC 1 cut(s) 1187
ErhI CCWWGG 1 cut(s) 1071
FaeI CATG 7 cut(s) 189, 211, 481, 913, 1042, 1118, 1158
FalI AAGNNNNNCTT 2 cut(s) 55, 87
FatI CATG 7 cut(s) 185, 207, 477, 909, 1038, 1114, 1154
FauI CCCGC 1 cut(s) 107
FbaI TGATCA 1 cut(s) 42
Fnu4HI GCNGC 3 cut(s) 6, 190, 618
FokI GGATG 2 cut(s) 42, 659
FriOI GRGCYC 1 cut(s) 1187
Fsp4HI GCNGC 3 cut(s) 6, 190, 618
FspBI CTAG 1 cut(s) 1119
GlaI GCGC 1 cut(s) 796
GluI GCNGC 3 cut(s) 6, 190, 618
GsaI CCCAGC 1 cut(s) 230
GsuI CTGGAG 1 cut(s) 653
HaeIII GGCC 2 cut(s) 969, 1070
HapII CCGG 3 cut(s) 110, 584, 831
HhaI GCGC 1 cut(s) 797
Hin1I GRCGYC 1 cut(s) 425
Hin1II CATG 7 cut(s) 189, 211, 481, 913, 1042, 1118, 1158
Hin6I GCGC 1 cut(s) 795
HinP1I GCGC 1 cut(s) 795
HincII GTYRAC 3 cut(s) 525, 669, 678
HindII GTYRAC 3 cut(s) 525, 669, 678
HinfI GANTC 4 cut(s) 117, 369, 472, 852
HpaII CCGG 3 cut(s) 110, 584, 831
HphI GGTGA 8 cut(s) 211, 373, 537, 543, 549, 598, 1167, 1233
Hpy166II GTNNAC 4 cut(s) 505, 525, 669, 678
Hpy188I TCNGA 6 cut(s) 116, 238, 609, 744, 872, 1004
Hpy188III TCNNGA 5 cut(s) 40, 208, 373, 692, 764
Hpy8I GTNNAC 4 cut(s) 505, 525, 669, 678
Hpy99I CGWCG 3 cut(s) 427, 430, 532
HpyAV CCTTC 6 cut(s) 343, 394, 445, 773, 867, 955
HpyCH4III ACNGT 6 cut(s) 318, 549, 555, 561, 567, 689
HpyCH4IV ACGT 2 cut(s) 425, 527
HpyCH4V TGCA 5 cut(s) 86, 189, 843, 1125, 1151
HpyF10VI GCNNNNNNNGC 1 cut(s) 419
HpyF3I CTNAG 5 cut(s) 461, 468, 765, 771, 925
HpySE526I ACGT 2 cut(s) 425, 527
Hsp92I GRCGYC 1 cut(s) 425
Hsp92II CATG 7 cut(s) 189, 211, 481, 913, 1042, 1118, 1158
HspAI GCGC 1 cut(s) 795
Ksp22I TGATCA 1 cut(s) 42
KspI CCGCGG 1 cut(s) 103
Kzo9I GATC 3 cut(s) 42, 739, 913
LmnI GCTCC 1 cut(s) 401
Lsp1109I GCAGC 2 cut(s) 201, 604
LweI GCATC 4 cut(s) 95, 267, 618, 852
MaeI CTAG 1 cut(s) 1119
MaeII ACGT 2 cut(s) 425, 527
MaeIII GTNAC 3 cut(s) 549, 555, 561
MalI GATC 3 cut(s) 44, 741, 915
MboI GATC 3 cut(s) 42, 739, 913
MflI RGATCY 1 cut(s) 913
MhlI GDGCHC 2 cut(s) 755, 1187
MluCI AATT 2 cut(s) 511, 1171
MlyI GAGTC 2 cut(s) 378, 846
MmeI TCCRAC 3 cut(s) 83, 261, 632
Mph1103I ATGCAT 1 cut(s) 845
MroXI GAANNNNTTC 1 cut(s) 783
MseI TTAA 3 cut(s) 726, 1055, 1140
MslI CAYNNNNRTG 1 cut(s) 818
MspA1I CMGCKG 2 cut(s) 102, 617
MspI CCGG 3 cut(s) 110, 584, 831
MspR9I CCNGG 2 cut(s) 171, 831
Mva1269I GAATGC 1 cut(s) 1153
MvaI CCWGG 1 cut(s) 171
MvnI CGCG 2 cut(s) 102, 422
MwoI GCNNNNNNNGC 1 cut(s) 419
NciI CCSGG 1 cut(s) 831
NdeII GATC 3 cut(s) 42, 739, 913
NlaIII CATG 7 cut(s) 189, 211, 481, 913, 1042, 1118, 1158
NlaIV GGNNCC 2 cut(s) 320, 606
NmeAIII GCCGAG 1 cut(s) 913
NmuCI GTSAC 2 cut(s) 549, 555
NsiI ATGCAT 1 cut(s) 845
NspI RCATGY 2 cut(s) 1042, 1158
NspV TTCGAA 1 cut(s) 133
PagI TCATGA 1 cut(s) 207
PciI ACATGT 1 cut(s) 1038
PcsI WCGNNNNNNNCGW 1 cut(s) 533
PctI GAATGC 1 cut(s) 1153
PdmI GAANNNNTTC 1 cut(s) 783
PfeI GAWTC 2 cut(s) 117, 472
PflFI GACNNNGTC 2 cut(s) 368, 673
PkrI GCNGC 3 cut(s) 7, 191, 619
PleI GAGTC 2 cut(s) 377, 846
PpsI GAGTC 2 cut(s) 377, 846
PscI ACATGT 1 cut(s) 1038
Psp6I CCWGG 1 cut(s) 169
PspFI CCCAGC 1 cut(s) 226
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 320, 606
PspPI GGNCC 3 cut(s) 104, 112, 968
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 1 cut(s) 913
PsyI GACNNNGTC 2 cut(s) 368, 673
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 3 cut(s) 161, 506, 1064
RsaNI GTAC 3 cut(s) 160, 505, 1063
RseI CAYNNNNRTG 1 cut(s) 818
Rsr2I CGGWCCG 1 cut(s) 112
RsrII CGGWCCG 1 cut(s) 112
SacII CCGCGG 1 cut(s) 103
SaqAI TTAA 3 cut(s) 726, 1055, 1140
SatI GCNGC 3 cut(s) 6, 190, 618
Sau3AI GATC 3 cut(s) 42, 739, 913
Sau96I GGNCC 3 cut(s) 104, 112, 968
SchI GAGTC 2 cut(s) 378, 846
ScrFI CCNGG 2 cut(s) 171, 831
SduI GDGCHC 2 cut(s) 755, 1187
SfaNI GCATC 4 cut(s) 95, 267, 618, 852
SfcI CTRYAG 1 cut(s) 612
Sfr303I CCGCGG 1 cut(s) 103
SfuI TTCGAA 1 cut(s) 133
SgrBI CCGCGG 1 cut(s) 103
SinI GGWCC 2 cut(s) 104, 112
SmiMI CAYNNNNRTG 1 cut(s) 818
Sse9I AATT 2 cut(s) 511, 1171
SsiI CCGC 2 cut(s) 100, 102
SspI AATATT 1 cut(s) 760
SspMI CTAG 1 cut(s) 1119
StyD4I CCNGG 2 cut(s) 169, 829
StyI CCWWGG 1 cut(s) 1071
TaaI ACNGT 6 cut(s) 318, 549, 555, 561, 567, 689
TaiI ACGT 2 cut(s) 428, 530
TaqI TCGA 6 cut(s) 133, 261, 363, 428, 593, 755
TaqII GACCGA 1 cut(s) 483
TasI AATT 2 cut(s) 511, 1171
TfiI GAWTC 2 cut(s) 117, 472
Tru1I TTAA 3 cut(s) 726, 1055, 1140
Tru9I TTAA 3 cut(s) 726, 1055, 1140
TscAI CASTG 3 cut(s) 308, 472, 576
TseFI GTSAC 2 cut(s) 549, 555
TseI GCWGC 3 cut(s) 5, 189, 617
Tsp45I GTSAC 2 cut(s) 549, 555
TspDTI ATGAA 4 cut(s) 196, 235, 427, 1160
TspRI CASTG 3 cut(s) 308, 472, 576
Tth111I GACNNNGTC 2 cut(s) 368, 673
VpaK11BI GGWCC 2 cut(s) 104, 112
XapI RAATTY 1 cut(s) 1171
XceI RCATGY 2 cut(s) 1042, 1158
XmnI GAANNNNTTC 1 cut(s) 783
XspI CTAG 1 cut(s) 1119
ZraI GACGTC 1 cut(s) 426
Zsp2I ATGCAT 1 cut(s) 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.