RchiOBHm_Chr7g0194801

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0013664
Physical Location & Seq
Forward (+)
12838895 .. 12839527
633 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17420

Sequence Viewer

Length: 630 bp
ATGCCTCTCTCACTAGCTAGACAGTCTTCACAGACAGGTAGCTTAGCTCTTCAACTCCACCGTACCCTCTCCTTATTCCATATGCCTCCATTACACATCTTCTTCTCCTTCTCACTCTTCTTCCTCTTTCCTCAAATAGCGAGGGCAGCTTCGGCCGACTGCTCCATATCAAGCTGCCCTAACGGCCCACCGGTCCGATTCCCTTTCCGGCTCAGACGCAAGCAGCGCCTTTTTTGTGGCTATCGAGGGTTCGACCTCTCATGCAACAACCAGACCCTACTCACCACGCTCACCCTCCCCGGCTCCGGAGCCTTCCTCGTCCAGCGCATCGATTACAAGTCTCAAAAGCTGTGGATCAACGACCCCAACTCCTGCCTCCCCAAAAGGCTCCTATCCCACCCCTTCAGCTTCTACGACACTCCTTTCAGTGTCGTTGGCGGTTCCAAGAACTACACCTTCCTCAACTGCTCGTCGTCTCAGACGACGTCAACGTTACCGGCCACCGCCACGAGTATTTCTTGCCTCAGCGGTGGGGGTACATGGTCTATGCTGTTCCCTCGAGTGCATACTATGCTTCTGCACCGGCAGCGGCACCGTCATCTTCGGCGTCACCGGAACTATGTTCGGTGA

Protein Analysis

209

Amino Acids

23.71

Weight (kDa)

11.04

Isoelectric Point (pI)

62.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 54 - 121 2e-16 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 488
AccB1I GGYRCC 1 cut(s) 591
AccIII TCCGGA 1 cut(s) 305
AciI CCGC 4 cut(s) 438, 504, 528, 589
AclI AACGTT 1 cut(s) 491
AclWI GGATC 1 cut(s) 362
AcoI YGGCCR 2 cut(s) 153, 498
AcuI CTGAAG 1 cut(s) 388
AcyI GRCGYC 2 cut(s) 485, 607
AfaI GTAC 2 cut(s) 64, 538
AfiI CCNNNNNNNGG 1 cut(s) 305
AgeI ACCGGT 1 cut(s) 190
AgsI TTSAA 1 cut(s) 53
AluBI AGCT 7 cut(s) 17, 42, 47, 149, 174, 349, 408
AluI AGCT 7 cut(s) 17, 42, 47, 149, 174, 349, 408
Alw26I GTCTC 2 cut(s) 345, 480
AlwI GGATC 1 cut(s) 362
Ama87I CYCGRG 1 cut(s) 558
Aor13HI TCCGGA 1 cut(s) 305
AoxI GGCC 3 cut(s) 153, 184, 498
ApeKI GCWGC 4 cut(s) 146, 174, 223, 586
AsiGI ACCGGT 1 cut(s) 190
AspLEI GCGC 2 cut(s) 228, 327
AspS9I GGNCC 2 cut(s) 185, 193
AsuC2I CCSGG 1 cut(s) 300
AsuHPI GGTGA 3 cut(s) 274, 283, 602
AvaI CYCGRG 1 cut(s) 558
AvaII GGWCC 1 cut(s) 193
BanI GGYRCC 1 cut(s) 591
BauI CACGAG 1 cut(s) 508
BbsI GAAGAC 1 cut(s) 18
BbvCI CCTCAGC 1 cut(s) 524
BbvI GCAGC 4 cut(s) 158, 161, 235, 598
BceAI ACGGC 1 cut(s) 199
BcnI CCSGG 1 cut(s) 300
BcoDI GTCTC 2 cut(s) 345, 480
BfaI CTAG 2 cut(s) 14, 18
BfoI RGCGCY 1 cut(s) 229
BglI GCCNNNNNGGC 1 cut(s) 183
BisI GCNGC 5 cut(s) 147, 175, 224, 587, 590
BlpI GCTNAGC 1 cut(s) 43
BlsI GCNGC 5 cut(s) 148, 176, 225, 588, 591
Bme1390I CCNGG 1 cut(s) 300
Bme18I GGWCC 1 cut(s) 193
BmeT110I CYCGRG 1 cut(s) 558
BmgT120I GGNCC 2 cut(s) 185, 193
BmiI GGNNCC 5 cut(s) 304, 310, 389, 442, 593
BmrFI CCNGG 1 cut(s) 300
BmsI GCATC 1 cut(s) 336
BpiI GAAGAC 1 cut(s) 18
BplI GAGNNNNNCTC 2 cut(s) 300, 332
Bpu10I CCTNAGC 1 cut(s) 524
Bpu1102I GCTNAGC 1 cut(s) 43
BpuMI CCSGG 1 cut(s) 300
Bsa29I ATCGAT 1 cut(s) 330
BsaHI GRCGYC 2 cut(s) 485, 607
BsaJI CCNNGG 1 cut(s) 298
BsaWI WCCGGW 3 cut(s) 190, 305, 612
BsaXI ACNNNNNCTCC 2 cut(s) 353, 383
Bsc4I CCNNNNNNNGG 1 cut(s) 305
Bse118I RCCGGY 3 cut(s) 190, 496, 582
BseAI TCCGGA 1 cut(s) 305
BseCI ATCGAT 1 cut(s) 330
BseDI CCNNGG 1 cut(s) 298
BseLI CCNNNNNNNGG 1 cut(s) 305
BseMII CTCAG 3 cut(s) 226, 491, 538
BseX3I CGGCCG 1 cut(s) 153
BseXI GCAGC 4 cut(s) 158, 161, 235, 598
BsgI GTGCAG 1 cut(s) 563
Bsh1285I CGRYCG 1 cut(s) 156
BshFI GGCC 3 cut(s) 155, 186, 500
BshNI GGYRCC 1 cut(s) 591
BshTI ACCGGT 1 cut(s) 190
BshVI ATCGAT 1 cut(s) 330
BsiEI CGRYCG 1 cut(s) 156
BsiHKCI CYCGRG 1 cut(s) 558
BsiSI CCGG 7 cut(s) 191, 208, 300, 306, 497, 583, 613
BslI CCNNNNNNNGG 1 cut(s) 305
BsmAI GTCTC 2 cut(s) 345, 480
BsmBI CGTCTC 1 cut(s) 480
BsnI GGCC 3 cut(s) 155, 186, 500
BsoBI CYCGRG 1 cut(s) 558
Bsp13I TCCGGA 1 cut(s) 305
Bsp143I GATC 1 cut(s) 354
Bsp1720I GCTNAGC 1 cut(s) 43
BspACI CCGC 4 cut(s) 438, 504, 528, 589
BspANI GGCC 3 cut(s) 155, 186, 500
BspCNI CTCAG 3 cut(s) 225, 490, 537
BspDI ATCGAT 1 cut(s) 330
BspEI TCCGGA 1 cut(s) 305
BspLI GGNNCC 5 cut(s) 304, 310, 389, 442, 593
BspPI GGATC 1 cut(s) 362
BspQI GCTCTTC 1 cut(s) 54
BspT107I GGYRCC 1 cut(s) 591
BsrFI RCCGGY 3 cut(s) 190, 496, 582
BssAI RCCGGY 3 cut(s) 190, 496, 582
BssECI CCNNGG 1 cut(s) 298
BssMI GATC 1 cut(s) 354
BssNI GRCGYC 2 cut(s) 485, 607
BssSI CACGAG 1 cut(s) 508
Bst2BI CACGAG 1 cut(s) 508
Bst4CI ACNGT 3 cut(s) 24, 62, 596
Bst6I CTCTTC 2 cut(s) 54, 122
BstACI GRCGYC 2 cut(s) 485, 607
BstAPI GCANNNNNTGC 1 cut(s) 571
BstC8I GCNNGC 1 cut(s) 221
BstDEI CTNAG 4 cut(s) 43, 212, 477, 524
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 2 cut(s) 228, 327
BstKTI GATC 1 cut(s) 357
BstMAI GTCTC 2 cut(s) 345, 480
BstMBI GATC 1 cut(s) 354
BstMCI CGRYCG 1 cut(s) 156
BstMWI GCNNNNNNNGC 6 cut(s) 146, 152, 183, 225, 571, 586
BstSCI CCNGG 1 cut(s) 298
BstV1I GCAGC 4 cut(s) 158, 161, 235, 598
BstV2I GAAGAC 1 cut(s) 18
BstZI CGGCCG 1 cut(s) 153
Bsu15I ATCGAT 1 cut(s) 330
BsuRI GGCC 3 cut(s) 155, 186, 500
BsuTUI ATCGAT 1 cut(s) 330
BtsIMutI CAGTG 1 cut(s) 433
Cac8I GCNNGC 1 cut(s) 221
CfoI GCGC 2 cut(s) 228, 327
Cfr10I RCCGGY 3 cut(s) 190, 496, 582
Cfr13I GGNCC 2 cut(s) 185, 193
ClaI ATCGAT 1 cut(s) 330
CpoI CGGWCCG 1 cut(s) 193
CseI GACGC 2 cut(s) 225, 596
Csp6I GTAC 2 cut(s) 63, 537
CspAI ACCGGT 1 cut(s) 190
CspCI CAANNNNNGTGG 2 cut(s) 332, 367
CspI CGGWCCG 1 cut(s) 193
CviAII CATG 2 cut(s) 261, 540
CviQI GTAC 2 cut(s) 63, 537
DdeI CTNAG 4 cut(s) 43, 212, 477, 524
DpnI GATC 1 cut(s) 356
DpnII GATC 1 cut(s) 354
EaeI YGGCCR 2 cut(s) 153, 498
EagI CGGCCG 1 cut(s) 153
Eam1104I CTCTTC 2 cut(s) 54, 122
EarI CTCTTC 2 cut(s) 54, 122
EclXI CGGCCG 1 cut(s) 153
Eco47I GGWCC 1 cut(s) 193
Eco52I CGGCCG 1 cut(s) 153
Eco57I CTGAAG 1 cut(s) 388
Eco88I CYCGRG 1 cut(s) 558
Esp3I CGTCTC 1 cut(s) 480
FaeI CATG 2 cut(s) 264, 543
FaiI YATR 9 cut(s) 81, 83, 167, 262, 541, 548, 567, 572, 621
FatI CATG 2 cut(s) 260, 539
FauNDI CATATG 1 cut(s) 81
Fnu4HI GCNGC 5 cut(s) 147, 175, 224, 587, 590
Fsp4HI GCNGC 5 cut(s) 147, 175, 224, 587, 590
FspBI CTAG 2 cut(s) 14, 18
GlaI GCGC 2 cut(s) 227, 326
GluI GCNGC 5 cut(s) 147, 175, 224, 587, 590
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 3 cut(s) 155, 186, 500
HapII CCGG 7 cut(s) 191, 208, 300, 306, 497, 583, 613
HgaI GACGC 2 cut(s) 225, 596
HhaI GCGC 2 cut(s) 228, 327
Hin1I GRCGYC 2 cut(s) 485, 607
Hin1II CATG 2 cut(s) 264, 543
Hin6I GCGC 2 cut(s) 226, 325
HinP1I GCGC 2 cut(s) 226, 325
HincII GTYRAC 1 cut(s) 489
HindII GTYRAC 1 cut(s) 489
HinfI GANTC 1 cut(s) 198
HpaII CCGG 7 cut(s) 191, 208, 300, 306, 497, 583, 613
HphI GGTGA 3 cut(s) 274, 283, 602
Hpy166II GTNNAC 1 cut(s) 489
Hpy188I TCNGA 3 cut(s) 197, 215, 480
Hpy188III TCNNGA 1 cut(s) 306
Hpy8I GTNNAC 1 cut(s) 489
Hpy99I CGWCG 2 cut(s) 475, 487
HpyAV CCTTC 4 cut(s) 118, 322, 412, 466
HpyCH4III ACNGT 3 cut(s) 24, 62, 596
HpyCH4IV ACGT 2 cut(s) 485, 491
HpyCH4V TGCA 3 cut(s) 264, 565, 580
HpyF10VI GCNNNNNNNGC 6 cut(s) 146, 152, 183, 225, 571, 586
HpyF3I CTNAG 4 cut(s) 43, 212, 477, 524
HpySE526I ACGT 2 cut(s) 485, 491
Hsp92I GRCGYC 2 cut(s) 485, 607
Hsp92II CATG 2 cut(s) 264, 543
HspAI GCGC 2 cut(s) 226, 325
Kpn2I TCCGGA 1 cut(s) 305
Kzo9I GATC 1 cut(s) 354
LguI GCTCTTC 1 cut(s) 54
LmnI GCTCC 4 cut(s) 167, 308, 308, 393
Lsp1109I GCAGC 4 cut(s) 158, 161, 235, 598
LweI GCATC 1 cut(s) 336
MaeI CTAG 2 cut(s) 14, 18
MaeII ACGT 2 cut(s) 485, 491
MaeIII GTNAC 2 cut(s) 492, 608
MalI GATC 1 cut(s) 356
MboI GATC 1 cut(s) 354
MboII GAAGA 7 cut(s) 18, 41, 91, 94, 109, 112, 593
MroI TCCGGA 1 cut(s) 305
MspA1I CMGCKG 2 cut(s) 528, 589
MspI CCGG 7 cut(s) 191, 208, 300, 306, 497, 583, 613
MspR9I CCNGG 1 cut(s) 300
MwoI GCNNNNNNNGC 6 cut(s) 146, 152, 183, 225, 571, 586
NciI CCSGG 1 cut(s) 300
NdeI CATATG 1 cut(s) 81
NdeII GATC 1 cut(s) 354
NlaIII CATG 2 cut(s) 264, 543
NlaIV GGNNCC 5 cut(s) 304, 310, 389, 442, 593
NmuCI GTSAC 1 cut(s) 608
PaeR7I CTCGAG 1 cut(s) 558
PciSI GCTCTTC 1 cut(s) 54
PcsI WCGNNNNNNNCGW 2 cut(s) 479, 488
PfeI GAWTC 1 cut(s) 198
PflFI GACNNNGTC 1 cut(s) 484
PinAI ACCGGT 1 cut(s) 190
PkrI GCNGC 5 cut(s) 148, 176, 225, 588, 591
Psp1406I AACGTT 1 cut(s) 491
PspN4I GGNNCC 5 cut(s) 304, 310, 389, 442, 593
PspPI GGNCC 2 cut(s) 185, 193
PspXI VCTCGAGB 1 cut(s) 558
PsyI GACNNNGTC 1 cut(s) 484
RsaI GTAC 2 cut(s) 64, 538
RsaNI GTAC 2 cut(s) 63, 537
Rsr2I CGGWCCG 1 cut(s) 193
RsrII CGGWCCG 1 cut(s) 193
SapI GCTCTTC 1 cut(s) 54
SatI GCNGC 5 cut(s) 147, 175, 224, 587, 590
Sau3AI GATC 1 cut(s) 354
Sau96I GGNCC 2 cut(s) 185, 193
ScrFI CCNGG 1 cut(s) 300
SfaNI GCATC 1 cut(s) 336
Sfr274I CTCGAG 1 cut(s) 558
SinI GGWCC 1 cut(s) 193
SlaI CTCGAG 1 cut(s) 558
SmlI CTYRAG 1 cut(s) 558
SmoI CTYRAG 1 cut(s) 558
SsiI CCGC 4 cut(s) 438, 504, 528, 589
SspMI CTAG 2 cut(s) 14, 18
StyD4I CCNGG 1 cut(s) 298
TaaI ACNGT 3 cut(s) 24, 62, 596
TaiI ACGT 2 cut(s) 488, 494
TaqI TCGA 4 cut(s) 244, 252, 330, 559
TauI GCSGC 1 cut(s) 592
TfiI GAWTC 1 cut(s) 198
TscAI CASTG 1 cut(s) 433
TseFI GTSAC 1 cut(s) 608
TseI GCWGC 4 cut(s) 146, 174, 223, 586
Tsp45I GTSAC 1 cut(s) 608
TspRI CASTG 1 cut(s) 433
Tth111I GACNNNGTC 1 cut(s) 484
VpaK11BI GGWCC 1 cut(s) 193
XhoI CTCGAG 1 cut(s) 558
XspI CTAG 2 cut(s) 14, 18
ZraI GACGTC 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.