Rw7G012480

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0013664
Physical Location & Seq
Forward (+)
11825738 .. 11828500
2763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G012480.1

Sequence Viewer

Length: 1215 bp
ATGCCTCCATTACACATCTTCTTCTCCTTCTCACTCTTCTTCCTCTTTCCTCAAATAGCGAGGGCAGCTTCGGCCGACTGCTCCATATCAAGCTGCCCTAACGGCCCACCGGTCCGATTCCCTTTCCGGCTCAGACGCAAGCAGCGCCTTTTTTGTGGCTATCGAGGGTTCGACCTCTCATGCAACAACCAGACCCTACTCACCACGCTCACCCTCCCCGGCTCCGGAGCCTTCCTCGTCCAGCGCATCGATTACAAGTCTCAAAAGCTGTGGATCAACGACCCCAACTCCTGCCTCCCCAAAAGGCTCCTATCCCACCCCTTCAGCTTCTACGACACTCCTTTCAGTGTCGTTGGCGGTTCCAAGAACTACACCTTCCTCAACTGCTCGTCGTCTCAGACGACGTCAACGTTACCGGCCACCGCCACGAGTATTTCTTGCCTCAGCGGTGGGGGGTACATGGTCTATGCTGTTCCCTCGAGTGCATACTATGCTTCTGCACCGGCAGCGGCACCGTCATCTTCGGCGTCACCGGAACTATGTTCGGTGATTTCGACTGCTTTGGTTCCGAGAGAAGTGACGTATGAGTGGGAAGTAAATATGGCTGGGGAGGTTACCCGCGGCGGTGTTGCCGTAACGTGGTCGCAACCGGATTGTAGTGATTGTGAAGCCAGGGGTAAAAATTGTGGGTTCGGCACAGATACAGGGCAGATCATGTGCTATGACCGCAAGAAAAGCCATGTTCTCCCAAGAGGTGCAAAATACGGCATCATCATAGGTGTGGGAATACCAGGGCTCTTATTCATCATTGCTATCGCAAATTACATCTACAGCAGGTTTTTTGGTCGCCGCCACCAGCCAAGCACAGACGACCTCTCCACCTCCACTGCTCTGCAATCCACCAACATCGTGGTGGGCCTTGACGCCCCAACCATTGAGTCATACCCCAAGACTTTGCTGGGGGAGAGTCGGAGATTGCCCAGGGCCAACGACAACACATGCTCAATTTGTCTGGGCGAGTACCAACCCAAAGAAGCACTGAGGACCATACCGGAATGCAACCACTACTTCCATGCTAATTGCATAGACGAATGGCTCAAACTGAATGCCACGTGCCCAGTATGCCGGAAGTCACCGGAGGGGTCATCTCATATCACCCCTTCTTCTTCAGTGTCCTCATCAACATCGTCTTCTTTAGCATCAGTAGTGCAATAG

Protein Analysis

404

Amino Acids

43.88

Weight (kDa)

8.67

Isoelectric Point (pI)

55.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 27 - 94 6.3e-16 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 171 - 242 3.6e-06 Wall-associated receptor kinase C-terminal
zf-RING_2 PF13639 332 - 376 1.9e-13 Ring finger domain
zf-RING_11 PF17123 334 - 362 3.4e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 334 - 375 4e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 334 - 376 1.7e-08 RING-H2 zinc finger domain
zf-C3HC4 PF00097 334 - 375 2.2e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 334 - 377 6.1e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 407
Acc36I ACCTGC 1 cut(s) 825
AccB1I GGYRCC 1 cut(s) 511
AccII CGCG 1 cut(s) 621
AccIII TCCGGA 1 cut(s) 224
AciI CCGC 9 cut(s) 357, 423, 447, 509, 619, 621, 624, 727, 850
AclI AACGTT 1 cut(s) 410
AclWI GGATC 1 cut(s) 281
AcoI YGGCCR 2 cut(s) 72, 417
AcuI CTGAAG 2 cut(s) 307, 1152
AcvI CACGTG 1 cut(s) 1113
AcyI GRCGYC 3 cut(s) 404, 527, 924
AfaI GTAC 2 cut(s) 458, 1022
AfiI CCNNNNNNNGG 2 cut(s) 224, 639
AgeI ACCGGT 1 cut(s) 109
AjnI CCWGG 3 cut(s) 671, 790, 980
AluBI AGCT 4 cut(s) 68, 93, 268, 327
AluI AGCT 4 cut(s) 68, 93, 268, 327
Alw26I GTCTC 2 cut(s) 264, 399
AlwI GGATC 1 cut(s) 281
Ama87I CYCGRG 1 cut(s) 478
Aor13HI TCCGGA 1 cut(s) 224
AoxI GGCC 5 cut(s) 72, 103, 417, 916, 984
ApeKI GCWGC 4 cut(s) 65, 93, 142, 506
AsiGI ACCGGT 1 cut(s) 109
AspLEI GCGC 2 cut(s) 147, 246
AspS9I GGNCC 5 cut(s) 104, 112, 916, 984, 1044
AsuC2I CCSGG 1 cut(s) 219
AsuHPI GGTGA 6 cut(s) 193, 202, 522, 559, 1125, 1147
AvaI CYCGRG 1 cut(s) 478
AvaII GGWCC 2 cut(s) 112, 1044
BaeGI GKGCMC 1 cut(s) 1118
BanI GGYRCC 1 cut(s) 511
BanII GRGCYC 1 cut(s) 798
BauI CACGAG 1 cut(s) 427
BbrPI CACGTG 1 cut(s) 1113
BbsI GAAGAC 1 cut(s) 1182
BbvCI CCTCAGC 1 cut(s) 443
BbvI GCAGC 4 cut(s) 77, 80, 154, 518
BceAI ACGGC 3 cut(s) 118, 617, 781
BciT130I CCWGG 3 cut(s) 673, 792, 982
BcnI CCSGG 1 cut(s) 219
BcoDI GTCTC 2 cut(s) 264, 399
BfmI CTRYAG 1 cut(s) 829
BfoI RGCGCY 1 cut(s) 148
BfuAI ACCTGC 1 cut(s) 825
BglI GCCNNNNNGGC 1 cut(s) 102
BisI GCNGC 7 cut(s) 66, 94, 143, 507, 510, 622, 850
BlsI GCNGC 7 cut(s) 67, 95, 144, 508, 511, 623, 851
Bme1390I CCNGG 4 cut(s) 219, 673, 792, 982
Bme18I GGWCC 2 cut(s) 112, 1044
BmeT110I CYCGRG 1 cut(s) 478
BmgT120I GGNCC 5 cut(s) 104, 112, 916, 984, 1044
BmiI GGNNCC 6 cut(s) 223, 229, 308, 361, 513, 567
BmrFI CCNGG 4 cut(s) 219, 673, 792, 982
BmrI ACTGGG 1 cut(s) 1112
BmsI GCATC 3 cut(s) 255, 777, 1208
BmuI ACTGGG 1 cut(s) 1112
BpiI GAAGAC 1 cut(s) 1182
BplI GAGNNNNNCTC 2 cut(s) 219, 251
Bpu10I CCTNAGC 1 cut(s) 443
BpuMI CCSGG 1 cut(s) 219
Bsa29I ATCGAT 1 cut(s) 249
BsaAI YACGTR 1 cut(s) 1113
BsaHI GRCGYC 3 cut(s) 404, 527, 924
BsaJI CCNNGG 6 cut(s) 217, 619, 672, 791, 980, 981
BsaWI WCCGGW 6 cut(s) 109, 224, 532, 649, 1051, 1135
BsaXI ACNNNNNCTCC 4 cut(s) 272, 302, 860, 890
Bsc4I CCNNNNNNNGG 2 cut(s) 224, 639
Bse118I RCCGGY 3 cut(s) 109, 415, 502
Bse1I ACTGG 1 cut(s) 1118
Bse3DI GCAATG 1 cut(s) 807
BseAI TCCGGA 1 cut(s) 224
BseBI CCWGG 3 cut(s) 673, 792, 982
BseCI ATCGAT 1 cut(s) 249
BseDI CCNNGG 6 cut(s) 217, 619, 672, 791, 980, 981
BseLI CCNNNNNNNGG 2 cut(s) 224, 639
BseMI GCAATG 1 cut(s) 807
BseMII CTCAG 4 cut(s) 145, 410, 457, 1031
BseNI ACTGG 1 cut(s) 1118
BseSI GKGCMC 1 cut(s) 1118
BseX3I CGGCCG 1 cut(s) 72
BseXI GCAGC 4 cut(s) 77, 80, 154, 518
BseYI CCCAGC 2 cut(s) 605, 958
BsgI GTGCAG 1 cut(s) 483
Bsh1236I CGCG 1 cut(s) 621
Bsh1285I CGRYCG 1 cut(s) 75
BshFI GGCC 5 cut(s) 74, 105, 419, 918, 986
BshNI GGYRCC 1 cut(s) 511
BshTI ACCGGT 1 cut(s) 109
BshVI ATCGAT 1 cut(s) 249
BsiEI CGRYCG 1 cut(s) 75
BsiHKCI CYCGRG 1 cut(s) 478
BslI CCNNNNNNNGG 2 cut(s) 224, 639
BsmAI GTCTC 2 cut(s) 264, 399
BsmBI CGTCTC 1 cut(s) 399
BsmI GAATGC 2 cut(s) 1061, 1111
BsnI GGCC 5 cut(s) 74, 105, 419, 918, 986
BsoBI CYCGRG 1 cut(s) 478
Bsp1286I GDGCHC 2 cut(s) 798, 1118
Bsp13I TCCGGA 1 cut(s) 224
Bsp143I GATC 2 cut(s) 273, 711
BspACI CCGC 9 cut(s) 357, 423, 447, 509, 619, 621, 624, 727, 850
BspANI GGCC 5 cut(s) 74, 105, 419, 918, 986
BspCNI CTCAG 4 cut(s) 144, 409, 456, 1032
BspDI ATCGAT 1 cut(s) 249
BspEI TCCGGA 1 cut(s) 224
BspFNI CGCG 1 cut(s) 621
BspLI GGNNCC 6 cut(s) 223, 229, 308, 361, 513, 567
BspMI ACCTGC 1 cut(s) 825
BspPI GGATC 1 cut(s) 281
BspT107I GGYRCC 1 cut(s) 511
BsrDI GCAATG 1 cut(s) 807
BsrFI RCCGGY 3 cut(s) 109, 415, 502
BsrI ACTGG 1 cut(s) 1118
BssAI RCCGGY 3 cut(s) 109, 415, 502
BssECI CCNNGG 6 cut(s) 217, 619, 672, 791, 980, 981
BssMI GATC 2 cut(s) 273, 711
BssNI GRCGYC 3 cut(s) 404, 527, 924
BssSI CACGAG 1 cut(s) 427
Bst2BI CACGAG 1 cut(s) 427
Bst2UI CCWGG 3 cut(s) 673, 792, 982
Bst4CI ACNGT 1 cut(s) 516
Bst6I CTCTTC 1 cut(s) 41
BstACI GRCGYC 3 cut(s) 404, 527, 924
BstAPI GCANNNNNTGC 1 cut(s) 491
BstBAI YACGTR 1 cut(s) 1113
BstC8I GCNNGC 1 cut(s) 140
BstDEI CTNAG 4 cut(s) 131, 396, 443, 1040
BstDSI CCRYGG 1 cut(s) 619
BstEII GGTNACC 1 cut(s) 613
BstFNI CGCG 1 cut(s) 621
BstH2I RGCGCY 1 cut(s) 148
BstHHI GCGC 2 cut(s) 147, 246
BstKTI GATC 2 cut(s) 276, 714
BstMAI GTCTC 2 cut(s) 264, 399
BstMBI GATC 2 cut(s) 273, 711
BstMCI CGRYCG 1 cut(s) 75
BstMWI GCNNNNNNNGC 9 cut(s) 65, 71, 102, 144, 491, 506, 726, 735, 1122
BstNI CCWGG 3 cut(s) 673, 792, 982
BstNSI RCATGY 1 cut(s) 1002
BstPI GGTNACC 1 cut(s) 613
BstSCI CCNGG 4 cut(s) 217, 671, 790, 980
BstSFI CTRYAG 1 cut(s) 829
BstSLI GKGCMC 1 cut(s) 1118
BstUI CGCG 1 cut(s) 621
BstV1I GCAGC 4 cut(s) 77, 80, 154, 518
BstV2I GAAGAC 1 cut(s) 1182
BstXI CCANNNNNNTGG 1 cut(s) 910
BstZI CGGCCG 1 cut(s) 72
Bsu15I ATCGAT 1 cut(s) 249
BsuRI GGCC 5 cut(s) 74, 105, 419, 918, 986
BsuTUI ATCGAT 1 cut(s) 249
BtgI CCRYGG 1 cut(s) 619
BtsI GCAGTG 1 cut(s) 885
BtsIMutI CAGTG 4 cut(s) 352, 885, 1037, 1176
BveI ACCTGC 1 cut(s) 825
Cac8I GCNNGC 1 cut(s) 140
CfoI GCGC 2 cut(s) 147, 246
Cfr10I RCCGGY 3 cut(s) 109, 415, 502
Cfr13I GGNCC 5 cut(s) 104, 112, 916, 984, 1044
Cfr42I CCGCGG 1 cut(s) 622
ClaI ATCGAT 1 cut(s) 249
CpoI CGGWCCG 1 cut(s) 112
CseI GACGC 3 cut(s) 144, 516, 932
Csp6I GTAC 2 cut(s) 457, 1021
CspAI ACCGGT 1 cut(s) 109
CspCI CAANNNNNGTGG 2 cut(s) 251, 286
CspI CGGWCCG 1 cut(s) 112
CviAII CATG 6 cut(s) 180, 460, 715, 740, 999, 1073
CviQI GTAC 2 cut(s) 457, 1021
DdeI CTNAG 4 cut(s) 131, 396, 443, 1040
DpnI GATC 2 cut(s) 275, 713
DpnII GATC 2 cut(s) 273, 711
EaeI YGGCCR 2 cut(s) 72, 417
EagI CGGCCG 1 cut(s) 72
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
EclXI CGGCCG 1 cut(s) 72
Eco24I GRGCYC 1 cut(s) 798
Eco47I GGWCC 2 cut(s) 112, 1044
Eco52I CGGCCG 1 cut(s) 72
Eco57I CTGAAG 2 cut(s) 307, 1152
Eco72I CACGTG 1 cut(s) 1113
Eco88I CYCGRG 1 cut(s) 478
Eco91I GGTNACC 1 cut(s) 613
EcoO65I GGTNACC 1 cut(s) 613
EcoRII CCWGG 3 cut(s) 671, 790, 980
EcoT38I GRGCYC 1 cut(s) 798
Esp3I CGTCTC 1 cut(s) 399
FaeI CATG 6 cut(s) 183, 463, 718, 743, 1002, 1076
FatI CATG 6 cut(s) 179, 459, 714, 739, 998, 1072
FauI CCCGC 1 cut(s) 626
Fnu4HI GCNGC 7 cut(s) 66, 94, 143, 507, 510, 622, 850
FriOI GRGCYC 1 cut(s) 798
Fsp4HI GCNGC 7 cut(s) 66, 94, 143, 507, 510, 622, 850
GlaI GCGC 2 cut(s) 146, 245
GluI GCNGC 7 cut(s) 66, 94, 143, 507, 510, 622, 850
GsaI CCCAGC 2 cut(s) 609, 962
HaeII RGCGCY 1 cut(s) 148
HaeIII GGCC 5 cut(s) 74, 105, 419, 918, 986
HgaI GACGC 3 cut(s) 144, 516, 932
HhaI GCGC 2 cut(s) 147, 246
Hin1I GRCGYC 3 cut(s) 404, 527, 924
Hin1II CATG 6 cut(s) 183, 463, 718, 743, 1002, 1076
Hin6I GCGC 2 cut(s) 145, 244
HinP1I GCGC 2 cut(s) 145, 244
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HinfI GANTC 3 cut(s) 117, 938, 967
HphI GGTGA 6 cut(s) 193, 202, 522, 559, 1125, 1147
Hpy166II GTNNAC 1 cut(s) 408
Hpy188I TCNGA 5 cut(s) 116, 134, 399, 570, 972
Hpy188III TCNNGA 1 cut(s) 225
Hpy8I GTNNAC 1 cut(s) 408
Hpy99I CGWCG 2 cut(s) 394, 406
HpyAV CCTTC 5 cut(s) 37, 241, 331, 385, 1170
HpyCH4III ACNGT 1 cut(s) 516
HpyCH4IV ACGT 5 cut(s) 404, 410, 581, 638, 1112
HpyCH4V TGCA 8 cut(s) 183, 485, 500, 758, 895, 1059, 1083, 1210
HpyF10VI GCNNNNNNNGC 9 cut(s) 65, 71, 102, 144, 491, 506, 726, 735, 1122
HpyF3I CTNAG 4 cut(s) 131, 396, 443, 1040
HpySE526I ACGT 5 cut(s) 404, 410, 581, 638, 1112
Hsp92I GRCGYC 3 cut(s) 404, 527, 924
Hsp92II CATG 6 cut(s) 183, 463, 718, 743, 1002, 1076
HspAI GCGC 2 cut(s) 145, 244
Kpn2I TCCGGA 1 cut(s) 224
KspI CCGCGG 1 cut(s) 622
Kzo9I GATC 2 cut(s) 273, 711
LmnI GCTCC 4 cut(s) 86, 227, 227, 312
Lsp1109I GCAGC 4 cut(s) 77, 80, 154, 518
LweI GCATC 3 cut(s) 255, 777, 1208
MaeII ACGT 5 cut(s) 404, 410, 581, 638, 1112
MaeIII GTNAC 6 cut(s) 411, 528, 577, 613, 634, 1131
MalI GATC 2 cut(s) 275, 713
MboI GATC 2 cut(s) 273, 711
MboII GAAGA 8 cut(s) 10, 13, 28, 31, 513, 1155, 1158, 1182
MhlI GDGCHC 2 cut(s) 798, 1118
MluCI AATT 4 cut(s) 682, 820, 1005, 1078
MlyI GAGTC 2 cut(s) 947, 976
MmeI TCCRAC 1 cut(s) 950
MroI TCCGGA 1 cut(s) 224
MslI CAYNNNNRTG 2 cut(s) 779, 911
MspA1I CMGCKG 3 cut(s) 447, 509, 621
MspR9I CCNGG 4 cut(s) 219, 673, 792, 982
Mva1269I GAATGC 2 cut(s) 1061, 1111
MvaI CCWGG 3 cut(s) 673, 792, 982
MvnI CGCG 1 cut(s) 621
MwoI GCNNNNNNNGC 9 cut(s) 65, 71, 102, 144, 491, 506, 726, 735, 1122
NciI CCSGG 1 cut(s) 219
NdeII GATC 2 cut(s) 273, 711
NlaIII CATG 6 cut(s) 183, 463, 718, 743, 1002, 1076
NlaIV GGNNCC 6 cut(s) 223, 229, 308, 361, 513, 567
NmuCI GTSAC 3 cut(s) 528, 577, 1131
NspI RCATGY 1 cut(s) 1002
PaeR7I CTCGAG 1 cut(s) 478
PasI CCCWGGG 1 cut(s) 981
PcsI WCGNNNNNNNCGW 3 cut(s) 398, 407, 551
PctI GAATGC 2 cut(s) 1061, 1111
PfeI GAWTC 1 cut(s) 117
PflFI GACNNNGTC 1 cut(s) 403
PinAI ACCGGT 1 cut(s) 109
PkrI GCNGC 7 cut(s) 67, 95, 144, 508, 511, 623, 851
PleI GAGTC 2 cut(s) 946, 975
PmaCI CACGTG 1 cut(s) 1113
PmlI CACGTG 1 cut(s) 1113
PpsI GAGTC 2 cut(s) 946, 975
Ppu21I YACGTR 1 cut(s) 1113
Psp1406I AACGTT 1 cut(s) 410
Psp6I CCWGG 3 cut(s) 671, 790, 980
PspCI CACGTG 1 cut(s) 1113
PspEI GGTNACC 1 cut(s) 613
PspFI CCCAGC 2 cut(s) 605, 958
PspGI CCWGG 3 cut(s) 671, 790, 980
PspN4I GGNNCC 6 cut(s) 223, 229, 308, 361, 513, 567
PspPI GGNCC 5 cut(s) 104, 112, 916, 984, 1044
PspXI VCTCGAGB 1 cut(s) 478
PsyI GACNNNGTC 1 cut(s) 403
RsaI GTAC 2 cut(s) 458, 1022
RsaNI GTAC 2 cut(s) 457, 1021
RseI CAYNNNNRTG 2 cut(s) 779, 911
Rsr2I CGGWCCG 1 cut(s) 112
RsrII CGGWCCG 1 cut(s) 112
SacII CCGCGG 1 cut(s) 622
SatI GCNGC 7 cut(s) 66, 94, 143, 507, 510, 622, 850
Sau3AI GATC 2 cut(s) 273, 711
Sau96I GGNCC 5 cut(s) 104, 112, 916, 984, 1044
SchI GAGTC 2 cut(s) 947, 976
ScrFI CCNGG 4 cut(s) 219, 673, 792, 982
SduI GDGCHC 2 cut(s) 798, 1118
SfaNI GCATC 3 cut(s) 255, 777, 1208
SfcI CTRYAG 1 cut(s) 829
Sfr274I CTCGAG 1 cut(s) 478
Sfr303I CCGCGG 1 cut(s) 622
SgrBI CCGCGG 1 cut(s) 622
SinI GGWCC 2 cut(s) 112, 1044
SlaI CTCGAG 1 cut(s) 478
SmiMI CAYNNNNRTG 2 cut(s) 779, 911
SmlI CTYRAG 1 cut(s) 478
SmoI CTYRAG 1 cut(s) 478
Sse9I AATT 4 cut(s) 682, 820, 1005, 1078
SsiI CCGC 9 cut(s) 357, 423, 447, 509, 619, 621, 624, 727, 850
StyD4I CCNGG 4 cut(s) 217, 671, 790, 980
TaaI ACNGT 1 cut(s) 516
TaiI ACGT 5 cut(s) 407, 413, 584, 641, 1115
TaqI TCGA 5 cut(s) 163, 171, 249, 479, 554
TasI AATT 4 cut(s) 682, 820, 1005, 1078
TauI GCSGC 3 cut(s) 512, 624, 852
TfiI GAWTC 1 cut(s) 117
TscAI CASTG 4 cut(s) 352, 892, 1044, 1176
TseFI GTSAC 3 cut(s) 528, 577, 1131
TseI GCWGC 4 cut(s) 65, 93, 142, 506
Tsp45I GTSAC 3 cut(s) 528, 577, 1131
TspDTI ATGAA 1 cut(s) 793
TspRI CASTG 4 cut(s) 352, 892, 1044, 1176
Tth111I GACNNNGTC 1 cut(s) 403
VpaK11BI GGWCC 2 cut(s) 112, 1044
XceI RCATGY 1 cut(s) 1002
XcmI CCANNNNNNNNNTGG 3 cut(s) 907, 910, 955
XhoI CTCGAG 1 cut(s) 478
ZraI GACGTC 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.