Rroxscaffold_3G00260970

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013664
Physical Location & Seq
Reverse (-)
55367308 .. 55370786
3479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00260970.1

Sequence Viewer

Length: 1224 bp
ATGCCTCCATTACACATCTTCTTCTCCTTCTCACTCTTCTTCCTCTTCTTCCTCTTTCCTCAAATAGCGAGGGCAGCTTCGGCCGACTGCTCCATATCAAGCTGCCCTAAAGGCCCACCAGTCCGATTCCCTTTCCGGCTCAGACGCATCCAGCCCCTTCTTTGTGGCTATCCAGGGTTCGACCTCTCATGCAACAACCAGACCCTACTCACCACGCTCACCCTCCGCGGCTCCGGAGCCTTCCTCGTCCTGCGCATCGATTACAAGTCTCAAAAGCTGTGGATCAACGACCCCAACTCCTGCCTCCCCAAAAGGCTCCTATTCCACCCCTTCAGCCTCCACGACACTCCGTTCAGTGTCGTTGGCGGTTCCAAGAACTACACCTTCCTCAACTGCTCGTCGTCTCAGACGACGTCAACGTTACCGGCCACCGCCACGAGTATTTCTTGTCTCAGCGGTGGGGGGTACACGGTCTATGCTGTTCCCTCGAGTGCATACTATGCTTCTGCACCGGCAGCCGCACCGTCATCTTCGGCGTCACCGGAATTATGTTCAGTGATTTCGACTGCTTTGGTTCCGAGAGAAGTGACGTATGAGTGGGAAGTACATATGGCTGGGGAAGTTACCCACGGCGGTGTTGCCCTGACGTGGTCTCGACCGGATTGTAGTGATTGTGAAGCCAGGGGTAAGAATTGTGGGTTCGACACAGATACAGGTCAGATCATCTGCTATGACCGCAAGAAAAGCCATGTTCTCCCAAGAGGTGCAAAATACGGCATAATCATAGGTGTGGGAATACCAGGGCTCTTATTCATCATTGCTATCGCAAATTACATCTACAGCAGGATTTTTGGTCGCCGCCACCAGCCAAGCACAGACGACCTCTCCACCTCCACTGCTCTGCAATCCGCCAACATCGTGGTGGGCCTTGATGCCCCAACCATTGAGTCATACCCCAAGACTTTGCTGGGGGAGAGTCGGAGATTGCCCAGGGCCAACGACAACACTTGCTCAATTTGTTTGGGCGAGTACCAACCCAAAGAAGCACTGAGGACCATACCGGAATGCAACCATTACTTCCATGCTAATTGCATAGACGAATGGCTCAAACTGAATGCCACGTGCCCAGTATGCCGGAAGTCACCGGAGGGGTCATCTCATGTCACCCCTTCTTCTTCAGTGTCCTCATCAACATCGTCTTCTTTAGCATCAGTAGTACAATAG

Protein Analysis

407

Amino Acids

44.16

Weight (kDa)

8.49

Isoelectric Point (pI)

53.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 30 - 97 4.6e-16 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 174 - 245 2.2e-06 Wall-associated receptor kinase C-terminal
zf-RING_2 PF13639 335 - 379 2e-13 Ring finger domain
zf-RING_11 PF17123 337 - 365 3.5e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 337 - 378 4.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 337 - 379 1.7e-08 RING-H2 zinc finger domain
zf-C3HC4 PF00097 337 - 378 2.2e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 337 - 380 6.2e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 416
Acc16I TGCGCA 1 cut(s) 254
AccII CGCG 1 cut(s) 228
AccIII TCCGGA 1 cut(s) 233
AclI AACGTT 1 cut(s) 419
AclWI GGATC 1 cut(s) 290
AcoI YGGCCR 2 cut(s) 81, 426
AcuI CTGAAG 2 cut(s) 316, 1161
AcvI CACGTG 1 cut(s) 1122
AcyI GRCGYC 2 cut(s) 413, 536
AfaI GTAC 4 cut(s) 467, 606, 1031, 1218
AfiI CCNNNNNNNGG 1 cut(s) 648
AjiI CACGTC 1 cut(s) 648
AjnI CCWGG 4 cut(s) 172, 680, 799, 989
AleI CACNNNNGTG 1 cut(s) 633
AluBI AGCT 3 cut(s) 77, 102, 277
AluI AGCT 3 cut(s) 77, 102, 277
Alw26I GTCTC 4 cut(s) 273, 408, 455, 657
AlwI GGATC 1 cut(s) 290
Ama87I CYCGRG 1 cut(s) 487
Aor13HI TCCGGA 1 cut(s) 233
AoxI GGCC 5 cut(s) 81, 112, 426, 925, 993
ApeKI GCWGC 3 cut(s) 74, 102, 515
AspLEI GCGC 1 cut(s) 255
AspS9I GGNCC 4 cut(s) 113, 925, 993, 1053
AsuHPI GGTGA 5 cut(s) 202, 211, 531, 1134, 1156
AvaI CYCGRG 1 cut(s) 487
AvaII GGWCC 1 cut(s) 1053
BaeGI GKGCMC 1 cut(s) 1127
BanII GRGCYC 1 cut(s) 807
BauI CACGAG 1 cut(s) 436
BbrPI CACGTG 1 cut(s) 1122
BbsI GAAGAC 1 cut(s) 1191
BbvI GCAGC 3 cut(s) 86, 89, 527
BceAI ACGGC 2 cut(s) 646, 790
BciT130I CCWGG 4 cut(s) 174, 682, 801, 991
BcoDI GTCTC 4 cut(s) 273, 408, 455, 657
BfmI CTRYAG 1 cut(s) 838
BglI GCCNNNNNGGC 1 cut(s) 111
BisI GCNGC 6 cut(s) 75, 103, 229, 516, 519, 859
BlsI GCNGC 6 cut(s) 76, 104, 230, 517, 520, 860
Bme1390I CCNGG 4 cut(s) 174, 682, 801, 991
Bme18I GGWCC 1 cut(s) 1053
BmeT110I CYCGRG 1 cut(s) 487
BmgBI CACGTC 1 cut(s) 648
BmgT120I GGNCC 4 cut(s) 113, 925, 993, 1053
BmiI GGNNCC 5 cut(s) 232, 238, 317, 370, 576
BmrFI CCNGG 4 cut(s) 174, 682, 801, 991
BmrI ACTGGG 1 cut(s) 1121
BmsI GCATC 4 cut(s) 156, 264, 922, 1217
BmuI ACTGGG 1 cut(s) 1121
BpiI GAAGAC 1 cut(s) 1191
BplI GAGNNNNNCTC 2 cut(s) 228, 260
Bsa29I ATCGAT 1 cut(s) 258
BsaAI YACGTR 1 cut(s) 1122
BsaHI GRCGYC 2 cut(s) 413, 536
BsaI GGTCTC 1 cut(s) 657
BsaJI CCNNGG 7 cut(s) 173, 226, 628, 681, 800, 989, 990
BsaWI WCCGGW 5 cut(s) 233, 541, 658, 1060, 1144
BsaXI ACNNNNNCTCC 4 cut(s) 281, 311, 869, 899
Bsc4I CCNNNNNNNGG 1 cut(s) 648
Bse118I RCCGGY 2 cut(s) 424, 511
Bse1I ACTGG 2 cut(s) 119, 1127
Bse3DI GCAATG 1 cut(s) 816
BseAI TCCGGA 1 cut(s) 233
BseBI CCWGG 4 cut(s) 174, 682, 801, 991
BseCI ATCGAT 1 cut(s) 258
BseDI CCNNGG 7 cut(s) 173, 226, 628, 681, 800, 989, 990
BseGI GGATG 1 cut(s) 147
BseLI CCNNNNNNNGG 1 cut(s) 648
BseMI GCAATG 1 cut(s) 816
BseMII CTCAG 4 cut(s) 154, 419, 466, 1040
BseNI ACTGG 2 cut(s) 119, 1127
BseSI GKGCMC 1 cut(s) 1127
BseX3I CGGCCG 1 cut(s) 81
BseXI GCAGC 3 cut(s) 86, 89, 527
BseYI CCCAGC 2 cut(s) 614, 967
BsgI GTGCAG 1 cut(s) 492
Bsh1236I CGCG 1 cut(s) 228
Bsh1285I CGRYCG 2 cut(s) 84, 659
BshFI GGCC 5 cut(s) 83, 114, 428, 927, 995
BshVI ATCGAT 1 cut(s) 258
BsiEI CGRYCG 2 cut(s) 84, 659
BsiHKCI CYCGRG 1 cut(s) 487
BsiSI CCGG 9 cut(s) 136, 234, 425, 512, 542, 659, 1061, 1135, 1145
BslI CCNNNNNNNGG 1 cut(s) 648
BsmAI GTCTC 4 cut(s) 273, 408, 455, 657
BsmBI CGTCTC 1 cut(s) 408
BsmI GAATGC 2 cut(s) 1070, 1120
BsnI GGCC 5 cut(s) 83, 114, 428, 927, 995
Bso31I GGTCTC 1 cut(s) 657
BsoBI CYCGRG 1 cut(s) 487
Bsp1286I GDGCHC 2 cut(s) 807, 1127
Bsp13I TCCGGA 1 cut(s) 233
Bsp143I GATC 2 cut(s) 282, 720
BspANI GGCC 5 cut(s) 83, 114, 428, 927, 995
BspCNI CTCAG 4 cut(s) 153, 418, 465, 1041
BspDI ATCGAT 1 cut(s) 258
BspEI TCCGGA 1 cut(s) 233
BspFNI CGCG 1 cut(s) 228
BspLI GGNNCC 5 cut(s) 232, 238, 317, 370, 576
BspPI GGATC 1 cut(s) 290
BspTNI GGTCTC 1 cut(s) 657
BsrDI GCAATG 1 cut(s) 816
BsrFI RCCGGY 2 cut(s) 424, 511
BsrI ACTGG 2 cut(s) 119, 1127
BssAI RCCGGY 2 cut(s) 424, 511
BssECI CCNNGG 7 cut(s) 173, 226, 628, 681, 800, 989, 990
BssMI GATC 2 cut(s) 282, 720
BssNI GRCGYC 2 cut(s) 413, 536
BssSI CACGAG 1 cut(s) 436
Bst2BI CACGAG 1 cut(s) 436
Bst2UI CCWGG 4 cut(s) 174, 682, 801, 991
Bst4CI ACNGT 2 cut(s) 472, 525
Bst6I CTCTTC 2 cut(s) 41, 50
BstACI GRCGYC 2 cut(s) 413, 536
BstAPI GCANNNNNTGC 1 cut(s) 500
BstBAI YACGTR 1 cut(s) 1122
BstDEI CTNAG 4 cut(s) 140, 405, 452, 1049
BstDSI CCRYGG 2 cut(s) 226, 628
BstF5I GGATG 1 cut(s) 147
BstFNI CGCG 1 cut(s) 228
BstHHI GCGC 1 cut(s) 255
BstKTI GATC 2 cut(s) 285, 723
BstMAI GTCTC 4 cut(s) 273, 408, 455, 657
BstMBI GATC 2 cut(s) 282, 720
BstMCI CGRYCG 2 cut(s) 84, 659
BstMWI GCNNNNNNNGC 8 cut(s) 74, 80, 111, 500, 515, 735, 744, 1131
BstNI CCWGG 4 cut(s) 174, 682, 801, 991
BstSCI CCNGG 4 cut(s) 172, 680, 799, 989
BstSFI CTRYAG 1 cut(s) 838
BstSLI GKGCMC 1 cut(s) 1127
BstUI CGCG 1 cut(s) 228
BstV1I GCAGC 3 cut(s) 86, 89, 527
BstV2I GAAGAC 1 cut(s) 1191
BstXI CCANNNNNNTGG 1 cut(s) 919
BstZI CGGCCG 1 cut(s) 81
Bsu15I ATCGAT 1 cut(s) 258
BsuRI GGCC 5 cut(s) 83, 114, 428, 927, 995
BsuTUI ATCGAT 1 cut(s) 258
BtgI CCRYGG 2 cut(s) 226, 628
BtrI CACGTC 1 cut(s) 648
BtsCI GGATG 1 cut(s) 147
BtsI GCAGTG 1 cut(s) 894
BtsIMutI CAGTG 5 cut(s) 361, 561, 894, 1046, 1185
CfoI GCGC 1 cut(s) 255
Cfr10I RCCGGY 2 cut(s) 424, 511
Cfr13I GGNCC 4 cut(s) 113, 925, 993, 1053
Cfr42I CCGCGG 1 cut(s) 229
ClaI ATCGAT 1 cut(s) 258
CseI GACGC 2 cut(s) 153, 525
Csp6I GTAC 4 cut(s) 466, 605, 1030, 1217
CspCI CAANNNNNGTGG 2 cut(s) 260, 295
CviAII CATG 4 cut(s) 189, 749, 1082, 1160
CviQI GTAC 4 cut(s) 466, 605, 1030, 1217
DdeI CTNAG 4 cut(s) 140, 405, 452, 1049
DpnI GATC 2 cut(s) 284, 722
DpnII GATC 2 cut(s) 282, 720
EaeI YGGCCR 2 cut(s) 81, 426
EagI CGGCCG 1 cut(s) 81
Eam1104I CTCTTC 2 cut(s) 41, 50
EarI CTCTTC 2 cut(s) 41, 50
EciI GGCGGA 1 cut(s) 898
EclXI CGGCCG 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 807
Eco31I GGTCTC 1 cut(s) 657
Eco47I GGWCC 1 cut(s) 1053
Eco52I CGGCCG 1 cut(s) 81
Eco57I CTGAAG 2 cut(s) 316, 1161
Eco72I CACGTG 1 cut(s) 1122
Eco88I CYCGRG 1 cut(s) 487
EcoRII CCWGG 4 cut(s) 172, 680, 799, 989
EcoT38I GRGCYC 1 cut(s) 807
Esp3I CGTCTC 1 cut(s) 408
FaeI CATG 4 cut(s) 192, 752, 1085, 1163
FatI CATG 4 cut(s) 188, 748, 1081, 1159
FauNDI CATATG 1 cut(s) 609
Fnu4HI GCNGC 6 cut(s) 75, 103, 229, 516, 519, 859
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 1 cut(s) 807
Fsp4HI GCNGC 6 cut(s) 75, 103, 229, 516, 519, 859
FspI TGCGCA 1 cut(s) 254
GlaI GCGC 1 cut(s) 254
GluI GCNGC 6 cut(s) 75, 103, 229, 516, 519, 859
GsaI CCCAGC 2 cut(s) 618, 971
HaeIII GGCC 5 cut(s) 83, 114, 428, 927, 995
HapII CCGG 9 cut(s) 136, 234, 425, 512, 542, 659, 1061, 1135, 1145
HgaI GACGC 2 cut(s) 153, 525
HhaI GCGC 1 cut(s) 255
Hin1I GRCGYC 2 cut(s) 413, 536
Hin1II CATG 4 cut(s) 192, 752, 1085, 1163
Hin6I GCGC 1 cut(s) 253
HinP1I GCGC 1 cut(s) 253
HincII GTYRAC 1 cut(s) 417
HindII GTYRAC 1 cut(s) 417
HinfI GANTC 3 cut(s) 126, 947, 976
HpaII CCGG 9 cut(s) 136, 234, 425, 512, 542, 659, 1061, 1135, 1145
HphI GGTGA 5 cut(s) 202, 211, 531, 1134, 1156
Hpy166II GTNNAC 2 cut(s) 417, 468
Hpy188I TCNGA 6 cut(s) 125, 143, 408, 579, 720, 981
Hpy188III TCNNGA 2 cut(s) 234, 654
Hpy8I GTNNAC 2 cut(s) 417, 468
Hpy99I CGWCG 2 cut(s) 403, 415
HpyAV CCTTC 6 cut(s) 37, 167, 250, 340, 394, 1179
HpyCH4III ACNGT 2 cut(s) 472, 525
HpyCH4IV ACGT 5 cut(s) 413, 419, 590, 647, 1121
HpyCH4V TGCA 7 cut(s) 192, 494, 509, 767, 904, 1068, 1092
HpyF10VI GCNNNNNNNGC 8 cut(s) 74, 80, 111, 500, 515, 735, 744, 1131
HpyF3I CTNAG 4 cut(s) 140, 405, 452, 1049
HpySE526I ACGT 5 cut(s) 413, 419, 590, 647, 1121
Hsp92I GRCGYC 2 cut(s) 413, 536
Hsp92II CATG 4 cut(s) 192, 752, 1085, 1163
HspAI GCGC 1 cut(s) 253
Kpn2I TCCGGA 1 cut(s) 233
KspI CCGCGG 1 cut(s) 229
Kzo9I GATC 2 cut(s) 282, 720
LmnI GCTCC 4 cut(s) 95, 236, 236, 321
Lsp1109I GCAGC 3 cut(s) 86, 89, 527
LweI GCATC 4 cut(s) 156, 264, 922, 1217
MaeII ACGT 5 cut(s) 413, 419, 590, 647, 1121
MaeIII GTNAC 6 cut(s) 420, 537, 586, 622, 1140, 1162
MalI GATC 2 cut(s) 284, 722
MboI GATC 2 cut(s) 282, 720
MhlI GDGCHC 2 cut(s) 807, 1127
MluCI AATT 5 cut(s) 545, 691, 829, 1014, 1087
MlyI GAGTC 2 cut(s) 956, 985
MmeI TCCRAC 1 cut(s) 959
MroI TCCGGA 1 cut(s) 233
MslI CAYNNNNRTG 3 cut(s) 633, 788, 920
MspA1I CMGCKG 2 cut(s) 228, 456
MspI CCGG 9 cut(s) 136, 234, 425, 512, 542, 659, 1061, 1135, 1145
MspR9I CCNGG 4 cut(s) 174, 682, 801, 991
Mva1269I GAATGC 2 cut(s) 1070, 1120
MvaI CCWGG 4 cut(s) 174, 682, 801, 991
MvnI CGCG 1 cut(s) 228
MwoI GCNNNNNNNGC 8 cut(s) 74, 80, 111, 500, 515, 735, 744, 1131
NdeI CATATG 1 cut(s) 609
NdeII GATC 2 cut(s) 282, 720
NlaIII CATG 4 cut(s) 192, 752, 1085, 1163
NlaIV GGNNCC 5 cut(s) 232, 238, 317, 370, 576
NmuCI GTSAC 4 cut(s) 537, 586, 1140, 1162
NsbI TGCGCA 1 cut(s) 254
OliI CACNNNNGTG 1 cut(s) 633
PaeR7I CTCGAG 1 cut(s) 487
PasI CCCWGGG 1 cut(s) 990
PcsI WCGNNNNNNNCGW 2 cut(s) 407, 416
PctI GAATGC 2 cut(s) 1070, 1120
PfeI GAWTC 1 cut(s) 126
PflFI GACNNNGTC 2 cut(s) 412, 649
PkrI GCNGC 6 cut(s) 76, 104, 230, 517, 520, 860
PleI GAGTC 2 cut(s) 955, 984
PmaCI CACGTG 1 cut(s) 1122
PmlI CACGTG 1 cut(s) 1122
PpsI GAGTC 2 cut(s) 955, 984
Ppu21I YACGTR 1 cut(s) 1122
Psp1406I AACGTT 1 cut(s) 419
Psp6I CCWGG 4 cut(s) 172, 680, 799, 989
PspCI CACGTG 1 cut(s) 1122
PspFI CCCAGC 2 cut(s) 614, 967
PspGI CCWGG 4 cut(s) 172, 680, 799, 989
PspN4I GGNNCC 5 cut(s) 232, 238, 317, 370, 576
PspPI GGNCC 4 cut(s) 113, 925, 993, 1053
PspXI VCTCGAGB 1 cut(s) 487
PsyI GACNNNGTC 2 cut(s) 412, 649
RsaI GTAC 4 cut(s) 467, 606, 1031, 1218
RsaNI GTAC 4 cut(s) 466, 605, 1030, 1217
RseI CAYNNNNRTG 3 cut(s) 633, 788, 920
SacII CCGCGG 1 cut(s) 229
SatI GCNGC 6 cut(s) 75, 103, 229, 516, 519, 859
Sau3AI GATC 2 cut(s) 282, 720
Sau96I GGNCC 4 cut(s) 113, 925, 993, 1053
SchI GAGTC 2 cut(s) 956, 985
ScrFI CCNGG 4 cut(s) 174, 682, 801, 991
SduI GDGCHC 2 cut(s) 807, 1127
SfaNI GCATC 4 cut(s) 156, 264, 922, 1217
SfcI CTRYAG 1 cut(s) 838
Sfr274I CTCGAG 1 cut(s) 487
Sfr303I CCGCGG 1 cut(s) 229
SgrBI CCGCGG 1 cut(s) 229
SinI GGWCC 1 cut(s) 1053
SlaI CTCGAG 1 cut(s) 487
SmiMI CAYNNNNRTG 3 cut(s) 633, 788, 920
SmlI CTYRAG 1 cut(s) 487
SmoI CTYRAG 1 cut(s) 487
Sse9I AATT 5 cut(s) 545, 691, 829, 1014, 1087
StyD4I CCNGG 4 cut(s) 172, 680, 799, 989
TaaI ACNGT 2 cut(s) 472, 525
TaiI ACGT 5 cut(s) 416, 422, 593, 650, 1124
TaqI TCGA 6 cut(s) 180, 258, 488, 563, 655, 702
TasI AATT 5 cut(s) 545, 691, 829, 1014, 1087
TatI WGTACW 2 cut(s) 604, 1216
TauI GCSGC 3 cut(s) 231, 521, 861
TfiI GAWTC 1 cut(s) 126
TscAI CASTG 5 cut(s) 361, 561, 901, 1053, 1185
TseFI GTSAC 4 cut(s) 537, 586, 1140, 1162
TseI GCWGC 3 cut(s) 74, 102, 515
Tsp45I GTSAC 4 cut(s) 537, 586, 1140, 1162
TspDTI ATGAA 1 cut(s) 802
TspGWI ACGGA 1 cut(s) 339
TspRI CASTG 5 cut(s) 361, 561, 901, 1053, 1185
Tth111I GACNNNGTC 2 cut(s) 412, 649
VpaK11BI GGWCC 1 cut(s) 1053
XcmI CCANNNNNNNNNTGG 2 cut(s) 919, 964
XhoI CTCGAG 1 cut(s) 487
ZraI GACGTC 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.