Rh7CG150100

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0013664
Physical Location & Seq
Forward (+)
12291420 .. 12294318
2899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG150100.1

Sequence Viewer

Length: 1296 bp
ATGCCTCTCTCACTAGCTAGACAGTCTTCACAGACAGGTAGCTTAGCTCTTCAACTCCACCGTACCCTCTCCTTATTCCATATGCCTCCATTACACATCTTCTTCTCCTTCTCACTCTTCTTCCTCTTTCCTCAAATAGCGAGGGCAGCTTCGGCCGACTGCTCCATATCAAGCTGCCCTAACGGCCCACCGGTTCGATTCCCTTTCCGGCTCAGACGCAAGCAGCCCCTTTTTTGTGGCTATCGAGGGTTCGACCTCTCATGCAACAACCAGACCCTACTCACCACGCTCACCCTCCCCGGCTCCGGAGCCTTCCTCGTCCAGCGCATCGATTACAAGTCTCAAAAGCTGTGGATCAACGACCCCAACTCCTGCCTCCCCAAAAGGCTCCTATCCCACCCCTTCAGCTTCCACAACACTCCTTTCAGTGTCGTTGGCGGTTCCAAGAACTACACCTTCCTCAACTGCTCGTCGTCTCAGACGACGTCAACGTTACCGGCCACCGCCACGAGTATTTCTTGCCTCAGCGGTGGGGGGTACATGGTCTATGCTGTTCCCTCAAGTGCATACTATGCTTCTGTACCGGCAGCGGCACCGTCATCTTCGGCGTCACCGGAACTATGTTCGGTGATTTCGACTGCTTTGGTTCCGAGAGAAGTGACGTATAAGTGGGAAGTAAATATGGCTGGGGAGGTTACCCGCGGCGGTGTTGCCGTGACGTGGTCGCAACCGGATTGTAGTGATTGTGAAGCTAGGGGTAAAAATTGTGGGTTCGACACAGATACAGGGCAGATCATGTGCTATGACCGCAAGAAAAGCCATGTTCTCCCAAGAGGTGCAAAATACGGCATAATCATAGGTGTGGGAATACCAGGGCTCTTATTCATCATTGCTATCGTAAATTACATCTACAGCAGGTTTTTTGGTCGCCGCCACCAGCCAAGCACAGACGATCTCTCCACCTCCACTGCTCTGCAATCCACCAACATCGTGGTGGGCCTTGACGCCCCAACCATTGAGTCATACCCCAAGACTTTGCTGGGGGAGAGTAGGAGATTGCCCAGGGCCAACGACAACACGTGCTCAATTTGTCTGGGCGAGTACCAACCCAAAGAAGCACTGAGGACCATACCGGAATGCAACCACTACTTCCATGCTAATTGCATAGACGAATGGCTCAAACTGAATGCCACATGCCCAGTATGCCGGAAGTCACCGGAGGGGTCATCTCATGTCACCCCTTCTTCTTCAGTGTCCTCATCAACATCGTCTTCTTTAGCATCAGTAGTGCAATAG

Protein Analysis

431

Amino Acids

46.87

Weight (kDa)

8.89

Isoelectric Point (pI)

55.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 54 - 121 1.1e-16 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 195 - 269 1e-06 Wall-associated receptor kinase C-terminal
zf-RING_2 PF13639 359 - 403 2.1e-13 Ring finger domain
zf-RING_11 PF17123 361 - 389 3.7e-10 RING-like zinc finger
zf-rbx1 PF12678 361 - 403 1.7e-08 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 361 - 402 4.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 361 - 402 2.3e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 361 - 404 6.7e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 488
Acc36I ACCTGC 1 cut(s) 906
AccB1I GGYRCC 1 cut(s) 592
AccII CGCG 1 cut(s) 702
AccIII TCCGGA 1 cut(s) 305
AciI CCGC 9 cut(s) 438, 504, 528, 590, 700, 702, 705, 808, 931
AclI AACGTT 1 cut(s) 491
AclWI GGATC 1 cut(s) 362
AcoI YGGCCR 2 cut(s) 153, 498
AcuI CTGAAG 2 cut(s) 388, 1233
AcvI CACGTG 1 cut(s) 1080
AcyI GRCGYC 3 cut(s) 485, 608, 1005
AfaI GTAC 4 cut(s) 64, 539, 582, 1103
AfiI CCNNNNNNNGG 2 cut(s) 305, 720
AflIII ACRYGT 1 cut(s) 1077
AgeI ACCGGT 1 cut(s) 190
AgsI TTSAA 1 cut(s) 53
AjiI CACGTC 1 cut(s) 720
AjnI CCWGG 2 cut(s) 871, 1061
AluBI AGCT 8 cut(s) 17, 42, 47, 149, 174, 349, 408, 752
AluI AGCT 8 cut(s) 17, 42, 47, 149, 174, 349, 408, 752
Alw21I GWGCWC 1 cut(s) 1085
Alw26I GTCTC 2 cut(s) 345, 480
AlwI GGATC 1 cut(s) 362
Aor13HI TCCGGA 1 cut(s) 305
AoxI GGCC 5 cut(s) 153, 184, 498, 997, 1065
ApeKI GCWGC 4 cut(s) 146, 174, 223, 587
AsiGI ACCGGT 1 cut(s) 190
AspLEI GCGC 1 cut(s) 327
AspS9I GGNCC 4 cut(s) 185, 997, 1065, 1125
AsuC2I CCSGG 1 cut(s) 300
AsuHPI GGTGA 6 cut(s) 274, 283, 603, 640, 1206, 1228
AvaII GGWCC 1 cut(s) 1125
BanI GGYRCC 1 cut(s) 592
BanII GRGCYC 1 cut(s) 879
BauI CACGAG 1 cut(s) 508
BbrPI CACGTG 1 cut(s) 1080
BbsI GAAGAC 2 cut(s) 18, 1263
Bbv12I GWGCWC 1 cut(s) 1085
BbvCI CCTCAGC 1 cut(s) 524
BbvI GCAGC 4 cut(s) 158, 161, 235, 599
BceAI ACGGC 3 cut(s) 199, 698, 862
BciT130I CCWGG 2 cut(s) 873, 1063
BcnI CCSGG 1 cut(s) 300
BcoDI GTCTC 2 cut(s) 345, 480
BfaI CTAG 3 cut(s) 14, 18, 753
BfmI CTRYAG 1 cut(s) 910
BfuAI ACCTGC 1 cut(s) 906
BglI GCCNNNNNGGC 1 cut(s) 183
BisI GCNGC 7 cut(s) 147, 175, 224, 588, 591, 703, 931
BlpI GCTNAGC 1 cut(s) 43
BlsI GCNGC 7 cut(s) 148, 176, 225, 589, 592, 704, 932
Bme1390I CCNGG 3 cut(s) 300, 873, 1063
Bme18I GGWCC 1 cut(s) 1125
BmgBI CACGTC 1 cut(s) 720
BmgT120I GGNCC 4 cut(s) 185, 997, 1065, 1125
BmiI GGNNCC 6 cut(s) 304, 310, 389, 442, 594, 648
BmrFI CCNGG 3 cut(s) 300, 873, 1063
BmrI ACTGGG 1 cut(s) 1193
BmsI GCATC 2 cut(s) 336, 1289
BmuI ACTGGG 1 cut(s) 1193
BpiI GAAGAC 2 cut(s) 18, 1263
BplI GAGNNNNNCTC 2 cut(s) 300, 332
Bpu10I CCTNAGC 1 cut(s) 524
Bpu1102I GCTNAGC 1 cut(s) 43
BpuEI CTTGAG 1 cut(s) 544
BpuMI CCSGG 1 cut(s) 300
Bsa29I ATCGAT 1 cut(s) 330
BsaAI YACGTR 1 cut(s) 1080
BsaHI GRCGYC 3 cut(s) 485, 608, 1005
BsaJI CCNNGG 5 cut(s) 298, 700, 872, 1061, 1062
BsaWI WCCGGW 6 cut(s) 190, 305, 613, 730, 1132, 1216
BsaXI ACNNNNNCTCC 4 cut(s) 353, 383, 941, 971
Bsc4I CCNNNNNNNGG 2 cut(s) 305, 720
Bse118I RCCGGY 3 cut(s) 190, 496, 583
Bse1I ACTGG 1 cut(s) 1199
Bse3DI GCAATG 1 cut(s) 888
BseAI TCCGGA 1 cut(s) 305
BseBI CCWGG 2 cut(s) 873, 1063
BseCI ATCGAT 1 cut(s) 330
BseDI CCNNGG 5 cut(s) 298, 700, 872, 1061, 1062
BseLI CCNNNNNNNGG 2 cut(s) 305, 720
BseMI GCAATG 1 cut(s) 888
BseMII CTCAG 4 cut(s) 226, 491, 538, 1112
BseNI ACTGG 1 cut(s) 1199
BseX3I CGGCCG 1 cut(s) 153
BseXI GCAGC 4 cut(s) 158, 161, 235, 599
BseYI CCCAGC 2 cut(s) 686, 1039
Bsh1236I CGCG 1 cut(s) 702
Bsh1285I CGRYCG 1 cut(s) 156
BshFI GGCC 5 cut(s) 155, 186, 500, 999, 1067
BshNI GGYRCC 1 cut(s) 592
BshTI ACCGGT 1 cut(s) 190
BshVI ATCGAT 1 cut(s) 330
BsiEI CGRYCG 1 cut(s) 156
BsiHKAI GWGCWC 1 cut(s) 1085
BslI CCNNNNNNNGG 2 cut(s) 305, 720
BsmAI GTCTC 2 cut(s) 345, 480
BsmBI CGTCTC 1 cut(s) 480
BsmI GAATGC 2 cut(s) 1142, 1192
BsnI GGCC 5 cut(s) 155, 186, 500, 999, 1067
Bsp1286I GDGCHC 2 cut(s) 879, 1085
Bsp13I TCCGGA 1 cut(s) 305
Bsp143I GATC 3 cut(s) 354, 792, 952
Bsp1720I GCTNAGC 1 cut(s) 43
BspACI CCGC 9 cut(s) 438, 504, 528, 590, 700, 702, 705, 808, 931
BspANI GGCC 5 cut(s) 155, 186, 500, 999, 1067
BspCNI CTCAG 4 cut(s) 225, 490, 537, 1113
BspDI ATCGAT 1 cut(s) 330
BspEI TCCGGA 1 cut(s) 305
BspFNI CGCG 1 cut(s) 702
BspLI GGNNCC 6 cut(s) 304, 310, 389, 442, 594, 648
BspMI ACCTGC 1 cut(s) 906
BspPI GGATC 1 cut(s) 362
BspQI GCTCTTC 1 cut(s) 54
BspT107I GGYRCC 1 cut(s) 592
BsrDI GCAATG 1 cut(s) 888
BsrFI RCCGGY 3 cut(s) 190, 496, 583
BsrI ACTGG 1 cut(s) 1199
BssAI RCCGGY 3 cut(s) 190, 496, 583
BssECI CCNNGG 5 cut(s) 298, 700, 872, 1061, 1062
BssMI GATC 3 cut(s) 354, 792, 952
BssNI GRCGYC 3 cut(s) 485, 608, 1005
BssSI CACGAG 1 cut(s) 508
Bst2BI CACGAG 1 cut(s) 508
Bst2UI CCWGG 2 cut(s) 873, 1063
Bst4CI ACNGT 3 cut(s) 24, 62, 597
Bst6I CTCTTC 2 cut(s) 54, 122
BstACI GRCGYC 3 cut(s) 485, 608, 1005
BstAPI GCANNNNNTGC 1 cut(s) 572
BstBAI YACGTR 1 cut(s) 1080
BstC8I GCNNGC 1 cut(s) 221
BstDEI CTNAG 5 cut(s) 43, 212, 477, 524, 1121
BstDSI CCRYGG 1 cut(s) 700
BstEII GGTNACC 1 cut(s) 694
BstFNI CGCG 1 cut(s) 702
BstHHI GCGC 1 cut(s) 327
BstKTI GATC 3 cut(s) 357, 795, 955
BstMAI GTCTC 2 cut(s) 345, 480
BstMBI GATC 3 cut(s) 354, 792, 952
BstMCI CGRYCG 1 cut(s) 156
BstMWI GCNNNNNNNGC 7 cut(s) 146, 152, 183, 572, 807, 816, 1203
BstNI CCWGG 2 cut(s) 873, 1063
BstNSI RCATGY 1 cut(s) 1197
BstPI GGTNACC 1 cut(s) 694
BstSCI CCNGG 3 cut(s) 298, 871, 1061
BstSFI CTRYAG 1 cut(s) 910
BstUI CGCG 1 cut(s) 702
BstV1I GCAGC 4 cut(s) 158, 161, 235, 599
BstV2I GAAGAC 2 cut(s) 18, 1263
BstXI CCANNNNNNTGG 1 cut(s) 991
BstZI CGGCCG 1 cut(s) 153
Bsu15I ATCGAT 1 cut(s) 330
BsuRI GGCC 5 cut(s) 155, 186, 500, 999, 1067
BsuTUI ATCGAT 1 cut(s) 330
BtgI CCRYGG 1 cut(s) 700
BtrI CACGTC 1 cut(s) 720
BtsI GCAGTG 1 cut(s) 966
BtsIMutI CAGTG 4 cut(s) 433, 966, 1118, 1257
BveI ACCTGC 1 cut(s) 906
Cac8I GCNNGC 1 cut(s) 221
CfoI GCGC 1 cut(s) 327
Cfr10I RCCGGY 3 cut(s) 190, 496, 583
Cfr13I GGNCC 4 cut(s) 185, 997, 1065, 1125
Cfr42I CCGCGG 1 cut(s) 703
ClaI ATCGAT 1 cut(s) 330
CseI GACGC 3 cut(s) 225, 597, 1013
Csp6I GTAC 4 cut(s) 63, 538, 581, 1102
CspAI ACCGGT 1 cut(s) 190
CspCI CAANNNNNGTGG 2 cut(s) 332, 367
CviAII CATG 7 cut(s) 261, 541, 796, 821, 1154, 1194, 1232
CviQI GTAC 4 cut(s) 63, 538, 581, 1102
DdeI CTNAG 5 cut(s) 43, 212, 477, 524, 1121
DpnI GATC 3 cut(s) 356, 794, 954
DpnII GATC 3 cut(s) 354, 792, 952
EaeI YGGCCR 2 cut(s) 153, 498
EagI CGGCCG 1 cut(s) 153
Eam1104I CTCTTC 2 cut(s) 54, 122
EarI CTCTTC 2 cut(s) 54, 122
EclXI CGGCCG 1 cut(s) 153
Eco24I GRGCYC 1 cut(s) 879
Eco47I GGWCC 1 cut(s) 1125
Eco52I CGGCCG 1 cut(s) 153
Eco57I CTGAAG 2 cut(s) 388, 1233
Eco72I CACGTG 1 cut(s) 1080
Eco91I GGTNACC 1 cut(s) 694
EcoO65I GGTNACC 1 cut(s) 694
EcoRII CCWGG 2 cut(s) 871, 1061
EcoT38I GRGCYC 1 cut(s) 879
Esp3I CGTCTC 1 cut(s) 480
FaeI CATG 7 cut(s) 264, 544, 799, 824, 1157, 1197, 1235
FatI CATG 7 cut(s) 260, 540, 795, 820, 1153, 1193, 1231
FauI CCCGC 1 cut(s) 707
FauNDI CATATG 1 cut(s) 81
Fnu4HI GCNGC 7 cut(s) 147, 175, 224, 588, 591, 703, 931
FriOI GRGCYC 1 cut(s) 879
Fsp4HI GCNGC 7 cut(s) 147, 175, 224, 588, 591, 703, 931
FspBI CTAG 3 cut(s) 14, 18, 753
GlaI GCGC 1 cut(s) 326
GluI GCNGC 7 cut(s) 147, 175, 224, 588, 591, 703, 931
GsaI CCCAGC 2 cut(s) 690, 1043
HaeIII GGCC 5 cut(s) 155, 186, 500, 999, 1067
HgaI GACGC 3 cut(s) 225, 597, 1013
HhaI GCGC 1 cut(s) 327
Hin1I GRCGYC 3 cut(s) 485, 608, 1005
Hin1II CATG 7 cut(s) 264, 544, 799, 824, 1157, 1197, 1235
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HincII GTYRAC 1 cut(s) 489
HindII GTYRAC 1 cut(s) 489
HinfI GANTC 2 cut(s) 198, 1019
HphI GGTGA 6 cut(s) 274, 283, 603, 640, 1206, 1228
Hpy166II GTNNAC 1 cut(s) 489
Hpy188I TCNGA 3 cut(s) 215, 480, 651
Hpy188III TCNNGA 1 cut(s) 306
Hpy8I GTNNAC 1 cut(s) 489
Hpy99I CGWCG 2 cut(s) 475, 487
HpyAV CCTTC 5 cut(s) 118, 322, 412, 466, 1251
HpyCH4III ACNGT 3 cut(s) 24, 62, 597
HpyCH4IV ACGT 5 cut(s) 485, 491, 662, 719, 1079
HpyCH4V TGCA 7 cut(s) 264, 566, 839, 976, 1140, 1164, 1291
HpyF10VI GCNNNNNNNGC 7 cut(s) 146, 152, 183, 572, 807, 816, 1203
HpyF3I CTNAG 5 cut(s) 43, 212, 477, 524, 1121
HpySE526I ACGT 5 cut(s) 485, 491, 662, 719, 1079
Hsp92I GRCGYC 3 cut(s) 485, 608, 1005
Hsp92II CATG 7 cut(s) 264, 544, 799, 824, 1157, 1197, 1235
HspAI GCGC 1 cut(s) 325
Kpn2I TCCGGA 1 cut(s) 305
KspI CCGCGG 1 cut(s) 703
Kzo9I GATC 3 cut(s) 354, 792, 952
LguI GCTCTTC 1 cut(s) 54
LmnI GCTCC 4 cut(s) 167, 308, 308, 393
Lsp1109I GCAGC 4 cut(s) 158, 161, 235, 599
LweI GCATC 2 cut(s) 336, 1289
MaeI CTAG 3 cut(s) 14, 18, 753
MaeII ACGT 5 cut(s) 485, 491, 662, 719, 1079
MaeIII GTNAC 7 cut(s) 492, 609, 658, 694, 715, 1212, 1234
MalI GATC 3 cut(s) 356, 794, 954
MboI GATC 3 cut(s) 354, 792, 952
MhlI GDGCHC 2 cut(s) 879, 1085
MluCI AATT 4 cut(s) 763, 901, 1086, 1159
MlyI GAGTC 1 cut(s) 1028
MroI TCCGGA 1 cut(s) 305
MslI CAYNNNNRTG 2 cut(s) 860, 992
MspA1I CMGCKG 3 cut(s) 528, 590, 702
MspR9I CCNGG 3 cut(s) 300, 873, 1063
Mva1269I GAATGC 2 cut(s) 1142, 1192
MvaI CCWGG 2 cut(s) 873, 1063
MvnI CGCG 1 cut(s) 702
MwoI GCNNNNNNNGC 7 cut(s) 146, 152, 183, 572, 807, 816, 1203
NciI CCSGG 1 cut(s) 300
NdeI CATATG 1 cut(s) 81
NdeII GATC 3 cut(s) 354, 792, 952
NlaIII CATG 7 cut(s) 264, 544, 799, 824, 1157, 1197, 1235
NlaIV GGNNCC 6 cut(s) 304, 310, 389, 442, 594, 648
NmuCI GTSAC 5 cut(s) 609, 658, 715, 1212, 1234
NspI RCATGY 1 cut(s) 1197
PasI CCCWGGG 1 cut(s) 1062
PciSI GCTCTTC 1 cut(s) 54
PcsI WCGNNNNNNNCGW 3 cut(s) 479, 488, 632
PctI GAATGC 2 cut(s) 1142, 1192
PfeI GAWTC 1 cut(s) 198
PflFI GACNNNGTC 2 cut(s) 484, 721
PinAI ACCGGT 1 cut(s) 190
PkrI GCNGC 7 cut(s) 148, 176, 225, 589, 592, 704, 932
PleI GAGTC 1 cut(s) 1027
PmaCI CACGTG 1 cut(s) 1080
PmlI CACGTG 1 cut(s) 1080
PpsI GAGTC 1 cut(s) 1027
Ppu21I YACGTR 1 cut(s) 1080
Psp1406I AACGTT 1 cut(s) 491
Psp6I CCWGG 2 cut(s) 871, 1061
PspCI CACGTG 1 cut(s) 1080
PspEI GGTNACC 1 cut(s) 694
PspFI CCCAGC 2 cut(s) 686, 1039
PspGI CCWGG 2 cut(s) 871, 1061
PspN4I GGNNCC 6 cut(s) 304, 310, 389, 442, 594, 648
PspPI GGNCC 4 cut(s) 185, 997, 1065, 1125
PsyI GACNNNGTC 2 cut(s) 484, 721
RsaI GTAC 4 cut(s) 64, 539, 582, 1103
RsaNI GTAC 4 cut(s) 63, 538, 581, 1102
RseI CAYNNNNRTG 2 cut(s) 860, 992
SacII CCGCGG 1 cut(s) 703
SapI GCTCTTC 1 cut(s) 54
SatI GCNGC 7 cut(s) 147, 175, 224, 588, 591, 703, 931
Sau3AI GATC 3 cut(s) 354, 792, 952
Sau96I GGNCC 4 cut(s) 185, 997, 1065, 1125
SchI GAGTC 1 cut(s) 1028
ScrFI CCNGG 3 cut(s) 300, 873, 1063
SduI GDGCHC 2 cut(s) 879, 1085
SfaNI GCATC 2 cut(s) 336, 1289
SfcI CTRYAG 1 cut(s) 910
Sfr303I CCGCGG 1 cut(s) 703
SgrBI CCGCGG 1 cut(s) 703
SinI GGWCC 1 cut(s) 1125
SmiMI CAYNNNNRTG 2 cut(s) 860, 992
SmlI CTYRAG 1 cut(s) 559
SmoI CTYRAG 1 cut(s) 559
Sse9I AATT 4 cut(s) 763, 901, 1086, 1159
SsiI CCGC 9 cut(s) 438, 504, 528, 590, 700, 702, 705, 808, 931
SspMI CTAG 3 cut(s) 14, 18, 753
StyD4I CCNGG 3 cut(s) 298, 871, 1061
TaaI ACNGT 3 cut(s) 24, 62, 597
TaiI ACGT 5 cut(s) 488, 494, 665, 722, 1082
TaqI TCGA 6 cut(s) 196, 244, 252, 330, 635, 774
TasI AATT 4 cut(s) 763, 901, 1086, 1159
TauI GCSGC 3 cut(s) 593, 705, 933
TfiI GAWTC 1 cut(s) 198
TscAI CASTG 4 cut(s) 433, 973, 1125, 1257
TseFI GTSAC 5 cut(s) 609, 658, 715, 1212, 1234
TseI GCWGC 4 cut(s) 146, 174, 223, 587
Tsp45I GTSAC 5 cut(s) 609, 658, 715, 1212, 1234
TspDTI ATGAA 1 cut(s) 874
TspRI CASTG 4 cut(s) 433, 973, 1125, 1257
Tth111I GACNNNGTC 2 cut(s) 484, 721
VpaK11BI GGWCC 1 cut(s) 1125
XceI RCATGY 1 cut(s) 1197
XcmI CCANNNNNNNNNTGG 3 cut(s) 988, 991, 1036
XspI CTAG 3 cut(s) 14, 18, 753
ZraI GACGTC 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.