Rh7BG146200

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0013664
Physical Location & Seq
Forward (+)
10847205 .. 10850167
2963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG146200.1

Sequence Viewer

Length: 1305 bp
ATGCCTCTCTCACTAGCTAGACAGTCTTCACAGACAGGTAGCTTAGCTCTTCAACTCCACCGTACCCTCTCCTTATTCCATATGCCTCCATTACACATCTTCTTCTCCTTCTCACTCTTCTTCCTCTTTCCTCAAATAGCGAGGGCAGCTTCGGCCGACTGCTCCATACAAAGCTGCCCTAACGGCCCACCGGTCCGATTCCCTTTCCGGCTCAGACGCATCCAGCCCGTTCTTTGTGGCTATCCAGGGTTCGACCTCTCATGCAACAACCAGACCCTACTCACCCTCCCCGGCTCCGGAGCCTTCCTCGTCCAGCGCATCGATTACAAGTCTCAAAAGCTGTGGATCGACGACCCCAACGCCTGCCTCCCCAAAAGGCTCCTATCCCACCCCTTCAGCCTCCATGACACTCCGTTCAGTGTCGTTGGCGGTTCCAAGAACTACACCTTCCTCAACTGCTCGTCGTCTCAGACGACGTCAACGTTACCGGCCACCGCCACGAGTATTTCTTGCCTCAGCGGTGGGGAGTACACGGTCTATGCTGTTCCTTCGAGTGCATACTATGCTTCTGCACCGGCAGCGGCACCGTCATCTTCGGCGTCACCGGAATTATGTTCGGTGATTTCGACTGCTTTGGTTCCGAGAGAAGTGACGACGTATGAGTGGGAAGTAAATATGGCTGGGGAGGTTACCCGCGGTGTTGCCGTGACGTGGTCGCAACCGGATTGTAGTGATTGTGAAGCCAGGGGTAAAAATTGTGGGTTCGACACAGATACAGTGACGATCATGTGCTATGACCGCAAGAAAAGCCATGTTCTCCCAAGAGGTGCAAAATACGGCATAATCATAGGTGTGGGAATACCAGGGCTCTTATTCATCATTGCTATCGCAAATTACATCTACAGCAGGTTTTTTGGTCGCCGCCACCAGCCAAGCACAGACGACCTCTCCACCTCCACTGCTCTGCAATCCACCAACATCGTGGTGGGCCTTGACGCCCCAACCATTGAGTCATACCCCAAGACTTTGCTGGGGGAGAGTCGGAGATTGCCCAGGGCCAACGACAACACGTGCTCAATTTGTTTGGGCGAGTACCAACCCAAAGAAGCACTGAGGACCATACCGGAATGCAACCACTACTTCCATGCTAATTGCATAGACGAATGGCTCAAACTGAATGCCACGTGCCCAGTATGCCGGAAGTCACCGGAGGGGTCATCTCATGTCACCCCTTCTTCTTCAGTCACCCCTTCTTCTTCAGTGTCCTCATCAACATCGTCTTCTTTAGCATCAGTAGTACAATAG

Protein Analysis

434

Amino Acids

47.03

Weight (kDa)

8.24

Isoelectric Point (pI)

55.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 54 - 118 8.3e-17 Wall-associated receptor kinase galacturonan-binding
zf-RING_2 PF13639 356 - 400 2.1e-13 Ring finger domain
zf-RING_11 PF17123 358 - 386 3.8e-10 RING-like zinc finger
zf-rbx1 PF12678 358 - 400 1.7e-08 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 358 - 399 4.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 358 - 399 2.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 358 - 401 6.8e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 479
Acc36I ACCTGC 1 cut(s) 897
AccB1I GGYRCC 1 cut(s) 583
AccII CGCG 1 cut(s) 696
AccIII TCCGGA 1 cut(s) 296
AciI CCGC 8 cut(s) 429, 495, 519, 581, 694, 696, 799, 922
AclI AACGTT 1 cut(s) 482
AclWI GGATC 1 cut(s) 353
AcoI YGGCCR 2 cut(s) 153, 489
AcuI CTGAAG 3 cut(s) 379, 1224, 1242
AcvI CACGTG 2 cut(s) 1071, 1185
AcyI GRCGYC 3 cut(s) 476, 599, 996
AfaI GTAC 4 cut(s) 64, 530, 1094, 1299
AfiI CCNNNNNNNGG 2 cut(s) 296, 711
AflIII ACRYGT 1 cut(s) 1068
AgeI ACCGGT 1 cut(s) 190
AgsI TTSAA 1 cut(s) 53
AjiI CACGTC 1 cut(s) 711
AjnI CCWGG 4 cut(s) 244, 743, 862, 1052
AluBI AGCT 6 cut(s) 17, 42, 47, 149, 174, 340
AluI AGCT 6 cut(s) 17, 42, 47, 149, 174, 340
Alw21I GWGCWC 1 cut(s) 1076
Alw26I GTCTC 2 cut(s) 336, 471
AlwI GGATC 1 cut(s) 353
Aor13HI TCCGGA 1 cut(s) 296
AoxI GGCC 5 cut(s) 153, 184, 489, 988, 1056
ApeKI GCWGC 3 cut(s) 146, 174, 578
AsiGI ACCGGT 1 cut(s) 190
AspLEI GCGC 1 cut(s) 318
AspS9I GGNCC 5 cut(s) 185, 193, 988, 1056, 1116
AsuC2I CCSGG 1 cut(s) 291
AsuHPI GGTGA 6 cut(s) 274, 594, 631, 1197, 1219, 1237
AvaII GGWCC 2 cut(s) 193, 1116
BaeGI GKGCMC 1 cut(s) 1190
BanI GGYRCC 1 cut(s) 583
BanII GRGCYC 1 cut(s) 870
BauI CACGAG 1 cut(s) 499
BbrPI CACGTG 2 cut(s) 1071, 1185
BbsI GAAGAC 2 cut(s) 18, 1272
Bbv12I GWGCWC 1 cut(s) 1076
BbvCI CCTCAGC 1 cut(s) 515
BbvI GCAGC 3 cut(s) 158, 161, 590
BceAI ACGGC 3 cut(s) 199, 689, 853
BciT130I CCWGG 4 cut(s) 246, 745, 864, 1054
BcnI CCSGG 1 cut(s) 291
BcoDI GTCTC 2 cut(s) 336, 471
BfaI CTAG 2 cut(s) 14, 18
BfmI CTRYAG 1 cut(s) 901
BfuAI ACCTGC 1 cut(s) 897
BglI GCCNNNNNGGC 1 cut(s) 183
BisI GCNGC 5 cut(s) 147, 175, 579, 582, 922
BlpI GCTNAGC 1 cut(s) 43
BlsI GCNGC 5 cut(s) 148, 176, 580, 583, 923
Bme1390I CCNGG 5 cut(s) 246, 291, 745, 864, 1054
Bme18I GGWCC 2 cut(s) 193, 1116
BmgBI CACGTC 1 cut(s) 711
BmgT120I GGNCC 5 cut(s) 185, 193, 988, 1056, 1116
BmiI GGNNCC 6 cut(s) 295, 301, 380, 433, 585, 639
BmrFI CCNGG 5 cut(s) 246, 291, 745, 864, 1054
BmrI ACTGGG 1 cut(s) 1184
BmsI GCATC 3 cut(s) 228, 327, 1298
BmuI ACTGGG 1 cut(s) 1184
BpiI GAAGAC 2 cut(s) 18, 1272
BplI GAGNNNNNCTC 2 cut(s) 291, 323
Bpu10I CCTNAGC 1 cut(s) 515
Bpu1102I GCTNAGC 1 cut(s) 43
BpuMI CCSGG 1 cut(s) 291
Bsa29I ATCGAT 1 cut(s) 321
BsaAI YACGTR 2 cut(s) 1071, 1185
BsaHI GRCGYC 3 cut(s) 476, 599, 996
BsaJI CCNNGG 7 cut(s) 245, 289, 694, 744, 863, 1052, 1053
BsaWI WCCGGW 6 cut(s) 190, 296, 604, 721, 1123, 1207
BsaXI ACNNNNNCTCC 4 cut(s) 270, 300, 932, 962
Bsc4I CCNNNNNNNGG 2 cut(s) 296, 711
Bse118I RCCGGY 3 cut(s) 190, 487, 574
Bse1I ACTGG 1 cut(s) 1190
Bse3DI GCAATG 1 cut(s) 879
BseAI TCCGGA 1 cut(s) 296
BseBI CCWGG 4 cut(s) 246, 745, 864, 1054
BseCI ATCGAT 1 cut(s) 321
BseDI CCNNGG 7 cut(s) 245, 289, 694, 744, 863, 1052, 1053
BseGI GGATG 1 cut(s) 219
BseLI CCNNNNNNNGG 2 cut(s) 296, 711
BseMI GCAATG 1 cut(s) 879
BseMII CTCAG 4 cut(s) 226, 482, 529, 1103
BseNI ACTGG 1 cut(s) 1190
BseSI GKGCMC 1 cut(s) 1190
BseX3I CGGCCG 1 cut(s) 153
BseXI GCAGC 3 cut(s) 158, 161, 590
BseYI CCCAGC 2 cut(s) 680, 1030
BsgI GTGCAG 1 cut(s) 555
Bsh1236I CGCG 1 cut(s) 696
Bsh1285I CGRYCG 1 cut(s) 156
BshFI GGCC 5 cut(s) 155, 186, 491, 990, 1058
BshNI GGYRCC 1 cut(s) 583
BshTI ACCGGT 1 cut(s) 190
BshVI ATCGAT 1 cut(s) 321
BsiEI CGRYCG 1 cut(s) 156
BsiHKAI GWGCWC 1 cut(s) 1076
BslI CCNNNNNNNGG 2 cut(s) 296, 711
BsmAI GTCTC 2 cut(s) 336, 471
BsmBI CGTCTC 1 cut(s) 471
BsmI GAATGC 2 cut(s) 1133, 1183
BsnI GGCC 5 cut(s) 155, 186, 491, 990, 1058
Bsp1286I GDGCHC 3 cut(s) 870, 1076, 1190
Bsp13I TCCGGA 1 cut(s) 296
Bsp143I GATC 2 cut(s) 345, 783
Bsp1720I GCTNAGC 1 cut(s) 43
BspACI CCGC 8 cut(s) 429, 495, 519, 581, 694, 696, 799, 922
BspANI GGCC 5 cut(s) 155, 186, 491, 990, 1058
BspCNI CTCAG 4 cut(s) 225, 481, 528, 1104
BspDI ATCGAT 1 cut(s) 321
BspEI TCCGGA 1 cut(s) 296
BspFNI CGCG 1 cut(s) 696
BspLI GGNNCC 6 cut(s) 295, 301, 380, 433, 585, 639
BspMI ACCTGC 1 cut(s) 897
BspPI GGATC 1 cut(s) 353
BspQI GCTCTTC 1 cut(s) 54
BspT107I GGYRCC 1 cut(s) 583
BsrDI GCAATG 1 cut(s) 879
BsrFI RCCGGY 3 cut(s) 190, 487, 574
BsrI ACTGG 1 cut(s) 1190
BssAI RCCGGY 3 cut(s) 190, 487, 574
BssECI CCNNGG 7 cut(s) 245, 289, 694, 744, 863, 1052, 1053
BssMI GATC 2 cut(s) 345, 783
BssNI GRCGYC 3 cut(s) 476, 599, 996
BssSI CACGAG 1 cut(s) 499
Bst2BI CACGAG 1 cut(s) 499
Bst2UI CCWGG 4 cut(s) 246, 745, 864, 1054
Bst4CI ACNGT 5 cut(s) 24, 62, 535, 588, 778
Bst6I CTCTTC 2 cut(s) 54, 122
BstACI GRCGYC 3 cut(s) 476, 599, 996
BstAPI GCANNNNNTGC 1 cut(s) 563
BstBAI YACGTR 2 cut(s) 1071, 1185
BstC8I GCNNGC 1 cut(s) 364
BstDEI CTNAG 5 cut(s) 43, 212, 468, 515, 1112
BstDSI CCRYGG 1 cut(s) 694
BstEII GGTNACC 1 cut(s) 688
BstF5I GGATG 1 cut(s) 219
BstFNI CGCG 1 cut(s) 696
BstHHI GCGC 1 cut(s) 318
BstKTI GATC 2 cut(s) 348, 786
BstMAI GTCTC 2 cut(s) 336, 471
BstMBI GATC 2 cut(s) 345, 783
BstMCI CGRYCG 1 cut(s) 156
BstMWI GCNNNNNNNGC 8 cut(s) 146, 152, 183, 563, 578, 798, 807, 1194
BstNI CCWGG 4 cut(s) 246, 745, 864, 1054
BstPI GGTNACC 1 cut(s) 688
BstSCI CCNGG 5 cut(s) 244, 289, 743, 862, 1052
BstSFI CTRYAG 1 cut(s) 901
BstSLI GKGCMC 1 cut(s) 1190
BstUI CGCG 1 cut(s) 696
BstV1I GCAGC 3 cut(s) 158, 161, 590
BstV2I GAAGAC 2 cut(s) 18, 1272
BstXI CCANNNNNNTGG 1 cut(s) 982
BstZI CGGCCG 1 cut(s) 153
Bsu15I ATCGAT 1 cut(s) 321
BsuRI GGCC 5 cut(s) 155, 186, 491, 990, 1058
BsuTUI ATCGAT 1 cut(s) 321
BtgI CCRYGG 1 cut(s) 694
BtrI CACGTC 1 cut(s) 711
BtsCI GGATG 1 cut(s) 219
BtsI GCAGTG 1 cut(s) 957
BtsIMutI CAGTG 5 cut(s) 424, 783, 957, 1109, 1266
BveI ACCTGC 1 cut(s) 897
Cac8I GCNNGC 1 cut(s) 364
CfoI GCGC 1 cut(s) 318
Cfr10I RCCGGY 3 cut(s) 190, 487, 574
Cfr13I GGNCC 5 cut(s) 185, 193, 988, 1056, 1116
Cfr42I CCGCGG 1 cut(s) 697
ClaI ATCGAT 1 cut(s) 321
CpoI CGGWCCG 1 cut(s) 193
CseI GACGC 3 cut(s) 225, 588, 1004
Csp6I GTAC 4 cut(s) 63, 529, 1093, 1298
CspAI ACCGGT 1 cut(s) 190
CspCI CAANNNNNGTGG 2 cut(s) 323, 358
CspI CGGWCCG 1 cut(s) 193
CviAII CATG 6 cut(s) 261, 404, 787, 812, 1145, 1223
CviQI GTAC 4 cut(s) 63, 529, 1093, 1298
DdeI CTNAG 5 cut(s) 43, 212, 468, 515, 1112
DpnI GATC 2 cut(s) 347, 785
DpnII GATC 2 cut(s) 345, 783
EaeI YGGCCR 2 cut(s) 153, 489
EagI CGGCCG 1 cut(s) 153
Eam1104I CTCTTC 2 cut(s) 54, 122
EarI CTCTTC 2 cut(s) 54, 122
EclXI CGGCCG 1 cut(s) 153
Eco24I GRGCYC 1 cut(s) 870
Eco47I GGWCC 2 cut(s) 193, 1116
Eco52I CGGCCG 1 cut(s) 153
Eco57I CTGAAG 3 cut(s) 379, 1224, 1242
Eco72I CACGTG 2 cut(s) 1071, 1185
Eco91I GGTNACC 1 cut(s) 688
EcoO65I GGTNACC 1 cut(s) 688
EcoRII CCWGG 4 cut(s) 244, 743, 862, 1052
EcoT38I GRGCYC 1 cut(s) 870
Esp3I CGTCTC 1 cut(s) 471
FaeI CATG 6 cut(s) 264, 407, 790, 815, 1148, 1226
FatI CATG 6 cut(s) 260, 403, 786, 811, 1144, 1222
FauI CCCGC 1 cut(s) 701
FauNDI CATATG 1 cut(s) 81
Fnu4HI GCNGC 5 cut(s) 147, 175, 579, 582, 922
FokI GGATG 1 cut(s) 206
FriOI GRGCYC 1 cut(s) 870
Fsp4HI GCNGC 5 cut(s) 147, 175, 579, 582, 922
FspBI CTAG 2 cut(s) 14, 18
GlaI GCGC 1 cut(s) 317
GluI GCNGC 5 cut(s) 147, 175, 579, 582, 922
GsaI CCCAGC 2 cut(s) 684, 1034
HaeIII GGCC 5 cut(s) 155, 186, 491, 990, 1058
HgaI GACGC 3 cut(s) 225, 588, 1004
HhaI GCGC 1 cut(s) 318
Hin1I GRCGYC 3 cut(s) 476, 599, 996
Hin1II CATG 6 cut(s) 264, 407, 790, 815, 1148, 1226
Hin6I GCGC 1 cut(s) 316
HinP1I GCGC 1 cut(s) 316
HincII GTYRAC 1 cut(s) 480
HindII GTYRAC 1 cut(s) 480
HinfI GANTC 3 cut(s) 198, 1010, 1039
HphI GGTGA 6 cut(s) 274, 594, 631, 1197, 1219, 1237
Hpy166II GTNNAC 2 cut(s) 480, 531
Hpy188I TCNGA 5 cut(s) 197, 215, 471, 642, 1044
Hpy188III TCNNGA 1 cut(s) 297
Hpy8I GTNNAC 2 cut(s) 480, 531
Hpy99I CGWCG 4 cut(s) 353, 466, 478, 658
HpyAV CCTTC 7 cut(s) 118, 313, 403, 457, 558, 1242, 1260
HpyCH4III ACNGT 5 cut(s) 24, 62, 535, 588, 778
HpyCH4IV ACGT 6 cut(s) 476, 482, 656, 710, 1070, 1184
HpyCH4V TGCA 7 cut(s) 264, 557, 572, 830, 967, 1131, 1155
HpyF10VI GCNNNNNNNGC 8 cut(s) 146, 152, 183, 563, 578, 798, 807, 1194
HpyF3I CTNAG 5 cut(s) 43, 212, 468, 515, 1112
HpySE526I ACGT 6 cut(s) 476, 482, 656, 710, 1070, 1184
Hsp92I GRCGYC 3 cut(s) 476, 599, 996
Hsp92II CATG 6 cut(s) 264, 407, 790, 815, 1148, 1226
HspAI GCGC 1 cut(s) 316
Kpn2I TCCGGA 1 cut(s) 296
KspI CCGCGG 1 cut(s) 697
Kzo9I GATC 2 cut(s) 345, 783
LguI GCTCTTC 1 cut(s) 54
LmnI GCTCC 4 cut(s) 167, 299, 299, 384
Lsp1109I GCAGC 3 cut(s) 158, 161, 590
LweI GCATC 3 cut(s) 228, 327, 1298
MaeI CTAG 2 cut(s) 14, 18
MaeII ACGT 6 cut(s) 476, 482, 656, 710, 1070, 1184
MaeIII GTNAC 9 cut(s) 483, 600, 649, 688, 706, 778, 1203, 1225, 1243
MalI GATC 2 cut(s) 347, 785
MboI GATC 2 cut(s) 345, 783
MhlI GDGCHC 3 cut(s) 870, 1076, 1190
MluCI AATT 5 cut(s) 608, 754, 892, 1077, 1150
MlyI GAGTC 2 cut(s) 1019, 1048
MmeI TCCRAC 1 cut(s) 1022
MroI TCCGGA 1 cut(s) 296
MslI CAYNNNNRTG 2 cut(s) 851, 983
MspA1I CMGCKG 3 cut(s) 519, 581, 696
MspR9I CCNGG 5 cut(s) 246, 291, 745, 864, 1054
Mva1269I GAATGC 2 cut(s) 1133, 1183
MvaI CCWGG 4 cut(s) 246, 745, 864, 1054
MvnI CGCG 1 cut(s) 696
MwoI GCNNNNNNNGC 8 cut(s) 146, 152, 183, 563, 578, 798, 807, 1194
NciI CCSGG 1 cut(s) 291
NdeI CATATG 1 cut(s) 81
NdeII GATC 2 cut(s) 345, 783
NlaIII CATG 6 cut(s) 264, 407, 790, 815, 1148, 1226
NlaIV GGNNCC 6 cut(s) 295, 301, 380, 433, 585, 639
NmuCI GTSAC 7 cut(s) 600, 649, 706, 778, 1203, 1225, 1243
PasI CCCWGGG 1 cut(s) 1053
PciSI GCTCTTC 1 cut(s) 54
PcsI WCGNNNNNNNCGW 3 cut(s) 470, 479, 623
PctI GAATGC 2 cut(s) 1133, 1183
PfeI GAWTC 1 cut(s) 198
PflFI GACNNNGTC 2 cut(s) 475, 712
PinAI ACCGGT 1 cut(s) 190
PkrI GCNGC 5 cut(s) 148, 176, 580, 583, 923
PleI GAGTC 2 cut(s) 1018, 1047
PmaCI CACGTG 2 cut(s) 1071, 1185
PmlI CACGTG 2 cut(s) 1071, 1185
PpsI GAGTC 2 cut(s) 1018, 1047
Ppu21I YACGTR 2 cut(s) 1071, 1185
Psp1406I AACGTT 1 cut(s) 482
Psp6I CCWGG 4 cut(s) 244, 743, 862, 1052
PspCI CACGTG 2 cut(s) 1071, 1185
PspEI GGTNACC 1 cut(s) 688
PspFI CCCAGC 2 cut(s) 680, 1030
PspGI CCWGG 4 cut(s) 244, 743, 862, 1052
PspN4I GGNNCC 6 cut(s) 295, 301, 380, 433, 585, 639
PspPI GGNCC 5 cut(s) 185, 193, 988, 1056, 1116
PsyI GACNNNGTC 2 cut(s) 475, 712
RsaI GTAC 4 cut(s) 64, 530, 1094, 1299
RsaNI GTAC 4 cut(s) 63, 529, 1093, 1298
RseI CAYNNNNRTG 2 cut(s) 851, 983
Rsr2I CGGWCCG 1 cut(s) 193
RsrII CGGWCCG 1 cut(s) 193
SacII CCGCGG 1 cut(s) 697
SapI GCTCTTC 1 cut(s) 54
SatI GCNGC 5 cut(s) 147, 175, 579, 582, 922
Sau3AI GATC 2 cut(s) 345, 783
Sau96I GGNCC 5 cut(s) 185, 193, 988, 1056, 1116
SchI GAGTC 2 cut(s) 1019, 1048
ScrFI CCNGG 5 cut(s) 246, 291, 745, 864, 1054
SduI GDGCHC 3 cut(s) 870, 1076, 1190
SfaNI GCATC 3 cut(s) 228, 327, 1298
SfcI CTRYAG 1 cut(s) 901
Sfr303I CCGCGG 1 cut(s) 697
SgrBI CCGCGG 1 cut(s) 697
SinI GGWCC 2 cut(s) 193, 1116
SmiMI CAYNNNNRTG 2 cut(s) 851, 983
Sse9I AATT 5 cut(s) 608, 754, 892, 1077, 1150
SsiI CCGC 8 cut(s) 429, 495, 519, 581, 694, 696, 799, 922
SspMI CTAG 2 cut(s) 14, 18
StyD4I CCNGG 5 cut(s) 244, 289, 743, 862, 1052
TaaI ACNGT 5 cut(s) 24, 62, 535, 588, 778
TaiI ACGT 6 cut(s) 479, 485, 659, 713, 1073, 1187
TaqI TCGA 6 cut(s) 252, 321, 348, 551, 626, 765
TasI AATT 5 cut(s) 608, 754, 892, 1077, 1150
TatI WGTACW 2 cut(s) 528, 1297
TauI GCSGC 2 cut(s) 584, 924
TfiI GAWTC 1 cut(s) 198
TscAI CASTG 5 cut(s) 424, 783, 964, 1116, 1266
TseFI GTSAC 7 cut(s) 600, 649, 706, 778, 1203, 1225, 1243
TseI GCWGC 3 cut(s) 146, 174, 578
Tsp45I GTSAC 7 cut(s) 600, 649, 706, 778, 1203, 1225, 1243
TspDTI ATGAA 1 cut(s) 865
TspGWI ACGGA 1 cut(s) 402
TspRI CASTG 5 cut(s) 424, 783, 964, 1116, 1266
Tth111I GACNNNGTC 2 cut(s) 475, 712
VpaK11BI GGWCC 2 cut(s) 193, 1116
XcmI CCANNNNNNNNNTGG 3 cut(s) 979, 982, 1027
XspI CTAG 2 cut(s) 14, 18
ZraI GACGTC 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.