Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0013822

Physical Location & Seq

Genomic Coordinates Reverse (-)

5709988 .. 5714671

Gene Structure 4684 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g10020.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2856 bp

ATGCATGTGTCGTCATCATCCTCCGTGCTGATATCATCTCCGCTTCGATGGAGAACTCCATCTCAACACGTTTCATTTACTCCCGCCGTTGCTTCCACCAGTACTACTTATGAACGCAGAAACGCCGTCGTTTCAATTCGCCTTGTAAATCCATTTTCTTCTTCTTCCTCGTTGTTCGCTACTCCCTTCCATGGCGGCCGGAGGAGAAAGACCTCGAAGCTCCGAGTCGTCTCCGCCGTCTTCGAGCGGTTCACGGAGCGCGCGATCAAAGCCGTCATCTTTTCCCAGAGAGAAGCCAAGGCTTTGGGAAGGGACATGGTGTTCACTCAGCACCTCCTGCTCGGTTTGATCGCCGAGGAGGAGCAGCATCGCCATCTTCATCCGAATTCGCTCGGGTTTCTCGGGTCGGGTATGACCCTGGACCAGGCCCGCCGCGCCGTCCGGAGCATTTGGCGGCATAATAGTAAAAGCAGTCAAAGTCAAAGTCAAGGTACTTCTGCTGGCTCTGGTTCGGGTTCGGCTACGGATCTGTCGTTTGCGATTAGCACGAAGAGAGTTCTGGAGGCTGCGCTGGAGTATTCGAGGAGTAGAGCTCATAATTTTCTTGCACCGGAGCACATTGTGATCGGTTTGTTGACTGTTGATGATGGAAGTGCTGGTCAGGTTCTTAAAAGACTGGGGGTAAATGTAAACCAGTTGTTAGCTGAGGCAGCCTCCAGACTTCAAATAGAGCTCGCCAAAGATGGCAGAGAGCCATCTGGGGGATCTCGAAAGACCTTTTCTAAAAAGTCTTCTGCATCGTCTGGGAAAACCAAAGAAAAAAGCGCTTTGGAACGGTTCTGTGTGGATCTCACTGCTCGTGCTAGCGAGGGACGCATTGATCCCGTTATTGGACGGGATACTGAAGTTCAGAGAATTATTCAGATACTTTGTCGCAGAACTAAAAATAATCCAATTCTTCTCGGTCAAAGTGGGGTTGGAAAGACGGCCATTGTTGAAGGTCTCGCAACTAGTATTGCACAGGCAGATGTCCCTGTTTATCTGTTGACAAAACGCGTAATGTCCTTGGACGTAGCACTATTAATGGCTGGTGCAAAGGAGAGGGGAGAGTTGGAGTCGCGTGTTACCTCCTTAATAAGTGATATACAAAAATCAGGAAATGTTATTCTATTTATTGATGAAGTTCATACCCTTATTGAGTCCGGCACAGTTGGACGAGGAAATAAGGGGTCTGGTCTTGACATTGCTAATATTATGAAACCGGCACTCGGGAGGGGTAAATTACAGTGTATTGCATCCACTACTACCGATGAATACAGGATGCATCTTGAAAAGGATAAAGCATTTGGACGAAGATTCCAACCTGTCTGGATTAATGAACCAAGCCAGGAGGATGCAGTTAGGATTCTATTTGGTCTGCGTGAGAGATATGAGGCCCATCACAACTGTATATATGCACCAGAAGCCATTAGTGCTGCTGTGTACCTGTCAGCAAGATATATTCCTGATAGATATCTTCCTGATAAAGCTATCGATCTCCTTGATGAGGCCGGAAGTAGAGCTCGTATGGAAGCCTTTAAGAAGAAAAAAGAAGAGCAGGTTGGCATACTTTCAAAGTCAGCGGATGATTATTGGCAAGAAATTCGTACAGTTCAGGCGATGCATGAAGTGGTCCTATCAAGTGAACTGAAATATGGAGCTGCTTCTGTGGACAATACCAGTGAACATATTCTAGACTCCGTTTCATCTTCCAAAGTAGATGATGAACCTACTGTGGTGGGACCAAATGATATAGCAGCAGTTGCCTCACTATGGTCAGGAGTCCCTCTTCAGCAGCTCACTGCTGATGACCGACTGCTTTTGGTGGGTCTTGATGAGAAGCTCAGAAGACGAGTTGTTGGTCAAGATGAGGCTGTTGCAGCCATTTCCCGAGCTGTTAGGAGGTCTCGGGTTGGATTGAAGGATCCAGGTAGACCAATGGCAACAATGCTATTTTGTGGTCCTACTGGAGTTGGTAAAACTGAACTGACAAAAGCTTTGGCTGCATCTTATTTTGGATCTGAGGAAGCTATGGTACGACTGGACATGAGTGAATATATGGAGCGGCATTCTGTCAGCAAATTAATAGGGTCACCCCCAGGCTATGTTGGCTTTGGAGAGGGAGGCACTCTAACAGAAGCTATCCGAAGACGACCTTTTACAGTAGTTGTGCTTGATGAAATCGAGAAGGCTCATCCAGATATATTCAACATCCTTCTCCAAATCTTTGAAGATGGCCACCTTACTGATTCTCAGGGACGGAGAGTATCATTTAAGAATGCTTTGGTGGTGATGACATCCAATGTGGGTTCCACTATCATTGCAAAGGGTCGACAAAGCTCCATTGGTTTTGTGCTTACAGATGATGAGTCTAGTTCATATGCTGGGATAAAAGCAACAGTAATGGAAGAACTCAAGTCATATTTTCGTCCGGAGTTGCTCAACAGGATAGACGAAGTAGTTGTATTCCATCCCCTTGAAAAGTCTCAGATGCTGGAGATTGTAAATATAATGCTACAAGAAGTGAAGCAGAGGCTAATGTCTTTGGGAATTGGATTAGACGTGTCTGAATCAGTCAAGGACCTCATATGCCAAGAAGGCTATGATCGGTTTTATGGTGCTCGCCCTCTTAGACGAGCAATTACATTAATAATTGAAGATCCATTGAGTGAATCCCTCCTTTCTGGAGTTTACCAGCCTGGTGACACTGCCGTCATTGATTTAGATGCTTCTGGGAACCCTTCTGTAAGTAATGGATCAGGTCAGAGTAGTTCCCCTTTCTCTAAAACCAGTATACAGCATCCATCTTGTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009941 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022607 GO:0031967 GO:0031975 GO:0033554 GO:0034214 GO:0034605 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046983 GO:0047484 GO:0048518 GO:0048583 GO:0048584 GO:0050789 GO:0050896 GO:0051259 GO:0051716 GO:0065003 GO:0065007 GO:0071840 GO:0080134 GO:1901000 GO:1901002 GO:1902584 GO:2000070

KEGG Pathways

Metabolic & Signaling

ko01100 map01100

Pfam Domains

Protein Families

AAA AAA_2 ClpB_D2-small Clp_N

Protein Analysis

952

Amino Acids

103.96

Weight (kDa)

8.75

Isoelectric Point (pI)

53.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	182 - 244	3.6e-10	Clp repeat (R) N-terminal domain
AAA_16	PF13191	296 - 350	6.3e-06	AAA ATPase domain
NBD_SMAX1	PF23569	302 - 403	1e-05	SMAX1 nucleotide binding domain
AAA	PF00004	319 - 435	4.5e-09	ATPase family associated with various cellular activities (AAA)
AAA_lid_9	PF17871	462 - 552	7.1e-27	AAA lid domain
AAA_2	PF07724	658 - 833	9.7e-64	AAA domain (Cdc48 subfamily)
AAA	PF00004	663 - 782	3.9e-06	ATPase family associated with various cellular activities (AAA)
AAA_5	PF07728	663 - 783	7.5e-11	AAA domain (dynein-related subfamily)
ClpB_D2-small	PF10431	839 - 918	6.3e-23	C-terminal, D2-small domain, of ClpB protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013822)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51070
fragaria_vesca	FvH4_5g10020
malus_domestica	MD06G1128400.v1.1
prunus_persica	Prupe.5G138200_v2.0.a1
pyrus_communis	pycom06g11860
rosa_chinensis	RchiOBHm_Chr7g0188241
rosa_laevigata	RLG00000004715
rosa_multiflora	Rmu_co8357845.1_g000001 Rmu_co8388145.1_g000001 Rmu_sc0008750.1_g000013
rosa_roxburghii	Rroxscaffold_3G00266580
rosa_rugosa	Rorug06G0489400
rosa_samantha	Rh7AG093700 Rh7BG095100 Rh7CG095500 Rh7DG095700
rosa_wichuraiana	Rw7G008070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2750
Acc36I	ACCTGC	1 cut(s)	1588
AccBSI	CCGCTC	2 cut(s)	247, 2104
AccI	GTMKAC	3 cut(s)	1972, 2371, 2833
AccII	CGCG	5 cut(s)	261, 263, 435, 1056, 1120
AccIII	TCCGGA	2 cut(s)	441, 2470
AclWI	GGATC	9 cut(s)	534, 772, 855, 875, 1958, 1971, 2065, 2693, 2803
AcoI	YGGCCR	3 cut(s)	196, 987, 2275
AcsI	RAATTY	2 cut(s)	385, 1641
AcuI	CTGAAG	2 cut(s)	924, 1814
AdeI	CACNNNGTG	1 cut(s)	622
AfaI	GTAC	5 cut(s)	103, 493, 1484, 1648, 2076
AfeI	AGCGCT	1 cut(s)	826
AfiI	CCNNNNNNNGG	7 cut(s)	191, 424, 761, 890, 1268, 1546, 1812
AflIII	ACRYGT	3 cut(s)	67, 1054, 2601
AhlI	ACTAGT	1 cut(s)	1010
AjiI	CACGTC	1 cut(s)	2602
AjnI	CCWGG	6 cut(s)	417, 423, 1386, 1966, 2137, 2737
AjuI	GAANNNNNNNTTGG	2 cut(s)	1584, 1616
AloI	GAACNNNNNNTCC	2 cut(s)	305, 337
Alw21I	GWGCWC	5 cut(s)	595, 618, 735, 1564, 2662
Alw26I	GTCTC	4 cut(s)	235, 1007, 1950, 2529
AlwI	GGATC	9 cut(s)	534, 772, 855, 875, 1958, 1971, 2065, 2693, 2803
AlwNI	CAGNNNCTG	1 cut(s)	2533
Ama87I	CYCGRG	5 cut(s)	392, 401, 1268, 1929, 1947
Aor13HI	TCCGGA	2 cut(s)	441, 2470
Aor51HI	AGCGCT	1 cut(s)	826
AoxI	GGCC	6 cut(s)	196, 426, 987, 1434, 1548, 2275
ApeKI	GCWGC	9 cut(s)	364, 566, 710, 1475, 1700, 1796, 1834, 1919, 2042
ApoI	RAATTY	2 cut(s)	385, 1641
ArsI	GACNNNNNNTTYG	4 cut(s)	469, 501, 2020, 2052
AseI	ATTAAT	4 cut(s)	1082, 1374, 2123, 2687
Asp700I	GAANNNNTTC	1 cut(s)	1728
AspLEI	GCGC	5 cut(s)	261, 263, 437, 571, 827
AspS9I	GGNCC	7 cut(s)	421, 427, 1435, 1672, 1781, 2000, 2620
AsuHPI	GGTGA	3 cut(s)	2124, 2341, 2753
AsuNHI	GCTAGC	1 cut(s)	863
AvaI	CYCGRG	5 cut(s)	392, 401, 1268, 1929, 1947
AvaII	GGWCC	5 cut(s)	421, 1672, 1781, 2000, 2620
BaeI	ACNNNNGTAYC	2 cut(s)	891, 924
BalI	TGGCCA	1 cut(s)	2277
BamHI	GGATCC	1 cut(s)	1963
BanII	GRGCYC	3 cut(s)	595, 735, 1564
BarI	GAAGNNNNNNTAC	2 cut(s)	2058, 2090
BauI	CACGAG	1 cut(s)	858
BbsI	GAAGAC	4 cut(s)	232, 783, 1894, 2194
Bbv12I	GWGCWC	5 cut(s)	595, 618, 735, 1564, 2662
BbvCI	CCTCAGC	1 cut(s)	705
BbvI	GCAGC	9 cut(s)	376, 553, 722, 1462, 1687, 1808, 1846, 1931, 2029
BceAI	ACGGC	7 cut(s)	71, 110, 221, 257, 422, 1002, 2735
BcgI	CGANNNNNNTGC	2 cut(s)	1625, 1659
BciT130I	CCWGG	6 cut(s)	419, 425, 1388, 1968, 2139, 2739
BciVI	GTATCC	1 cut(s)	892
BcoDI	GTCTC	4 cut(s)	235, 1007, 1950, 2529
BcuI	ACTAGT	1 cut(s)	1010
BfaI	CTAG	4 cut(s)	864, 1011, 1733, 2412
BfoI	RGCGCY	1 cut(s)	828
BfuAI	ACCTGC	1 cut(s)	1588
BfuI	GTATCC	1 cut(s)	892
BglI	GCCNNNNNGGC	1 cut(s)	2637
BmcAI	AGTACT	1 cut(s)	103
Bme1390I	CCNGG	6 cut(s)	419, 425, 1388, 1968, 2139, 2739
Bme18I	GGWCC	5 cut(s)	421, 1672, 1781, 2000, 2620
BmeT110I	CYCGRG	5 cut(s)	392, 401, 1268, 1929, 1947
BmgBI	CACGTC	1 cut(s)	2602
BmgT120I	GGNCC	7 cut(s)	421, 427, 1435, 1672, 1781, 2000, 2620
BmiI	GGNNCC	4 cut(s)	1782, 1965, 2350, 2777
BmrFI	CCNGG	6 cut(s)	419, 425, 1388, 1968, 2139, 2739
BmrI	ACTGGG	1 cut(s)	686
BmtI	GCTAGC	1 cut(s)	867
BmuI	ACTGGG	1 cut(s)	686
BpiI	GAAGAC	4 cut(s)	232, 783, 1894, 2194
BplI	GAGNNNNNCTC	4 cut(s)	577, 609, 698, 730
BpmI	CTGGAG	6 cut(s)	581, 593, 700, 2028, 2555, 2745
Bpu10I	CCTNAGC	1 cut(s)	705
BpuEI	CTTGAG	1 cut(s)	2438
Bsa29I	ATCGAT	1 cut(s)	1533
BsaBI	GATNNNNATC	1 cut(s)	1512
BsaI	GGTCTC	2 cut(s)	1007, 1950
BsaJI	CCNNGG	6 cut(s)	190, 297, 354, 417, 1065, 2137
BsaWI	WCCGGW	3 cut(s)	441, 610, 2470
Bsc4I	CCNNNNNNNGG	7 cut(s)	191, 424, 761, 890, 1268, 1546, 1812
Bse118I	RCCGGY	1 cut(s)	1261
Bse1I	ACTGG	7 cut(s)	99, 681, 694, 1719, 2011, 2085, 2829
Bse3DI	GCAATG	2 cut(s)	1242, 2358
Bse8I	GATNNNNATC	1 cut(s)	1512
BseAI	TCCGGA	2 cut(s)	441, 2470
BseBI	CCWGG	6 cut(s)	419, 425, 1388, 1968, 2139, 2739
BseCI	ATCGAT	1 cut(s)	1533
BseDI	CCNNGG	6 cut(s)	190, 297, 354, 417, 1065, 2137
BseJI	GATNNNNATC	1 cut(s)	1512
BseLI	CCNNNNNNNGG	7 cut(s)	191, 424, 761, 890, 1268, 1546, 1812
BseMI	GCAATG	2 cut(s)	1242, 2358
BseMII	CTCAG	6 cut(s)	341, 696, 1897, 2052, 2306, 2540
BseNI	ACTGG	7 cut(s)	99, 681, 694, 1719, 2011, 2085, 2829
BsePI	GCGCGC	1 cut(s)	259
BseRI	GAGGAG	4 cut(s)	217, 371, 374, 598
BseX3I	CGGCCG	1 cut(s)	196
BseXI	GCAGC	9 cut(s)	376, 553, 722, 1462, 1687, 1808, 1846, 1931, 2029
BseYI	CCCAGC	1 cut(s)	2423
Bsh1236I	CGCG	5 cut(s)	261, 263, 435, 1056, 1120
Bsh1285I	CGRYCG	1 cut(s)	199
BshFI	GGCC	6 cut(s)	198, 428, 989, 1436, 1550, 2277
BshVI	ATCGAT	1 cut(s)	1533
BsiEI	CGRYCG	1 cut(s)	199
BsiHKAI	GWGCWC	5 cut(s)	595, 618, 735, 1564, 2662
BsiHKCI	CYCGRG	5 cut(s)	392, 401, 1268, 1929, 1947
BsiSI	CCGG	7 cut(s)	199, 442, 611, 1203, 1262, 1551, 2471
BslFI	GGGAC	6 cut(s)	326, 885, 1016, 1794, 1808, 2310
BslI	CCNNNNNNNGG	7 cut(s)	191, 424, 761, 890, 1268, 1546, 1812
BsmAI	GTCTC	4 cut(s)	235, 1007, 1950, 2529
BsmBI	CGTCTC	1 cut(s)	235
BsmFI	GGGAC	6 cut(s)	326, 885, 1016, 1794, 1808, 2310
BsmI	GAATGC	2 cut(s)	2107, 2323
BsnI	GGCC	6 cut(s)	198, 428, 989, 1436, 1550, 2277
Bso31I	GGTCTC	2 cut(s)	1007, 1950
BsoBI	CYCGRG	5 cut(s)	392, 401, 1268, 1929, 1947
Bsp1286I	GDGCHC	5 cut(s)	595, 618, 735, 1564, 2662
Bsp13I	TCCGGA	2 cut(s)	441, 2470
Bsp19I	CCATGG	1 cut(s)	190
BspANI	GGCC	6 cut(s)	198, 428, 989, 1436, 1550, 2277
BspCNI	CTCAG	6 cut(s)	340, 697, 1896, 2053, 2305, 2539
BspDI	ATCGAT	1 cut(s)	1533
BspEI	TCCGGA	2 cut(s)	441, 2470
BspFNI	CGCG	5 cut(s)	261, 263, 435, 1056, 1120
BspLI	GGNNCC	4 cut(s)	1782, 1965, 2350, 2777
BspMI	ACCTGC	1 cut(s)	1588
BspOI	GCTAGC	1 cut(s)	867
BspPI	GGATC	9 cut(s)	534, 772, 855, 875, 1958, 1971, 2065, 2693, 2803
BspQI	GCTCTTC	1 cut(s)	1587
BspTNI	GGTCTC	2 cut(s)	1007, 1950
BsrBI	CCGCTC	2 cut(s)	247, 2104
BsrDI	GCAATG	2 cut(s)	1242, 2358
BsrFI	RCCGGY	1 cut(s)	1261
BsrI	ACTGG	7 cut(s)	99, 681, 694, 1719, 2011, 2085, 2829
BssAI	RCCGGY	1 cut(s)	1261
BssECI	CCNNGG	6 cut(s)	190, 297, 354, 417, 1065, 2137
BssHII	GCGCGC	1 cut(s)	259
BssNAI	GTATAC	1 cut(s)	2834
BssSI	CACGAG	1 cut(s)	858
BssT1I	CCWWGG	3 cut(s)	190, 297, 1065
Bst1107I	GTATAC	1 cut(s)	2834
Bst2BI	CACGAG	1 cut(s)	858
Bst2UI	CCWGG	6 cut(s)	419, 425, 1388, 1968, 2139, 2739
Bst4CI	ACNGT	9 cut(s)	640, 837, 1210, 1287, 1448, 1651, 1774, 2203, 2440
Bst6I	CTCTTC	3 cut(s)	545, 1587, 1833
BstAPI	GCANNNNNTGC	2 cut(s)	337, 1802
BstC8I	GCNNGC	6 cut(s)	261, 430, 502, 735, 865, 2662
BstDEI	CTNAG	7 cut(s)	327, 705, 1883, 2061, 2292, 2526, 2669
BstDSI	CCRYGG	1 cut(s)	190
BstEII	GGTNACC	1 cut(s)	2130
BstENI	CCTNNNNNAGG	2 cut(s)	422, 1544
BstFNI	CGCG	5 cut(s)	261, 263, 435, 1056, 1120
BstH2I	RGCGCY	1 cut(s)	828
BstHHI	GCGC	5 cut(s)	261, 263, 437, 571, 827
BstMAI	GTCTC	4 cut(s)	235, 1007, 1950, 2529
BstMCI	CGRYCG	1 cut(s)	199
BstNI	CCWGG	6 cut(s)	419, 425, 1388, 1968, 2139, 2739
BstNSI	RCATGY	1 cut(s)	8
BstPI	GGTNACC	1 cut(s)	2130
BstSCI	CCNGG	6 cut(s)	417, 423, 1386, 1966, 2137, 2737
BstUI	CGCG	5 cut(s)	261, 263, 435, 1056, 1120
BstV1I	GCAGC	9 cut(s)	376, 553, 722, 1462, 1687, 1808, 1846, 1931, 2029
BstV2I	GAAGAC	4 cut(s)	232, 783, 1894, 2194
BstX2I	RGATCY	6 cut(s)	526, 764, 847, 1963, 2057, 2698
BstXI	CCANNNNNNTGG	1 cut(s)	304
BstYI	RGATCY	6 cut(s)	526, 764, 847, 1963, 2057, 2698
BstZ17I	GTATAC	1 cut(s)	2834
BstZI	CGGCCG	1 cut(s)	196
Bsu15I	ATCGAT	1 cut(s)	1533
BsuI	GTATCC	1 cut(s)	892
BsuRI	GGCC	6 cut(s)	198, 428, 989, 1436, 1550, 2277
BsuTUI	ATCGAT	1 cut(s)	1533
BtgI	CCRYGG	1 cut(s)	190
BtgZI	GCGATG	2 cut(s)	353, 1673
BtrI	CACGTC	1 cut(s)	2602
BtsI	GCAGTG	3 cut(s)	852, 1839, 2745
BtsIMutI	CAGTG	5 cut(s)	852, 1292, 1726, 1839, 2745
BveI	ACCTGC	1 cut(s)	1588
Cac8I	GCNNGC	6 cut(s)	261, 430, 502, 735, 865, 2662
CaiI	CAGNNNCTG	1 cut(s)	2533
CfoI	GCGC	5 cut(s)	261, 263, 437, 571, 827
Cfr10I	RCCGGY	1 cut(s)	1261
Cfr13I	GGNCC	7 cut(s)	421, 427, 1435, 1672, 1781, 2000, 2620
ClaI	ATCGAT	1 cut(s)	1533
CseI	GACGC	1 cut(s)	882
Csp6I	GTAC	5 cut(s)	102, 492, 1483, 1647, 2075
CspCI	CAANNNNNGTGG	2 cut(s)	2341, 2376
CviAII	CATG	5 cut(s)	5, 191, 316, 1664, 2086
CviQI	GTAC	5 cut(s)	102, 492, 1483, 1647, 2075
DdeI	CTNAG	7 cut(s)	327, 705, 1883, 2061, 2292, 2526, 2669
DraIII	CACNNNGTG	1 cut(s)	622
DrdI	GACNNNNNNGTC	1 cut(s)	2750
DseDI	GACNNNNNNGTC	1 cut(s)	2750
EaeI	YGGCCR	3 cut(s)	196, 987, 2275
EagI	CGGCCG	1 cut(s)	196
Eam1104I	CTCTTC	3 cut(s)	545, 1587, 1833
EarI	CTCTTC	3 cut(s)	545, 1587, 1833
EciI	GGCGGA	1 cut(s)	223
Ecl136II	GAGCTC	3 cut(s)	593, 733, 1562
EclXI	CGGCCG	1 cut(s)	196
Eco130I	CCWWGG	3 cut(s)	190, 297, 1065
Eco24I	GRGCYC	3 cut(s)	595, 735, 1564
Eco31I	GGTCTC	2 cut(s)	1007, 1950
Eco32I	GATATC	2 cut(s)	33, 1514
Eco47I	GGWCC	5 cut(s)	421, 1672, 1781, 2000, 2620
Eco47III	AGCGCT	1 cut(s)	826
Eco52I	CGGCCG	1 cut(s)	196
Eco53kI	GAGCTC	3 cut(s)	593, 733, 1562
Eco57I	CTGAAG	2 cut(s)	924, 1814
Eco88I	CYCGRG	5 cut(s)	392, 401, 1268, 1929, 1947
Eco91I	GGTNACC	1 cut(s)	2130
EcoICRI	GAGCTC	3 cut(s)	593, 733, 1562
EcoNI	CCTNNNNNAGG	2 cut(s)	422, 1544
EcoO109I	RGGNCCY	1 cut(s)	2620
EcoO65I	GGTNACC	1 cut(s)	2130
EcoRI	GAATTC	1 cut(s)	385
EcoRII	CCWGG	6 cut(s)	417, 423, 1386, 1966, 2137, 2737
EcoRV	GATATC	2 cut(s)	33, 1514
EcoT14I	CCWWGG	3 cut(s)	190, 297, 1065
EcoT22I	ATGCAT	3 cut(s)	6, 1326, 1665
EcoT38I	GRGCYC	3 cut(s)	595, 735, 1564
ErhI	CCWWGG	3 cut(s)	190, 297, 1065
Esp3I	CGTCTC	1 cut(s)	235
FaeI	CATG	5 cut(s)	8, 194, 319, 1667, 2089
FalI	AAGNNNNNCTT	2 cut(s)	2179, 2211
FaqI	GGGAC	6 cut(s)	326, 885, 1016, 1794, 1808, 2310
FatI	CATG	5 cut(s)	4, 190, 315, 1663, 2085
FauI	CCCGC	2 cut(s)	91, 437
FauNDI	CATATG	2 cut(s)	2419, 2627
FblI	GTMKAC	3 cut(s)	1972, 2371, 2833
FriOI	GRGCYC	3 cut(s)	595, 735, 1564
FspBI	CTAG	4 cut(s)	864, 1011, 1733, 2412
GlaI	GCGC	5 cut(s)	260, 262, 436, 570, 826
GsaI	CCCAGC	1 cut(s)	2427
GsuI	CTGGAG	6 cut(s)	581, 593, 700, 2028, 2555, 2745
HaeII	RGCGCY	1 cut(s)	828
HaeIII	GGCC	6 cut(s)	198, 428, 989, 1436, 1550, 2277
HapII	CCGG	7 cut(s)	199, 442, 611, 1203, 1262, 1551, 2471
HgaI	GACGC	1 cut(s)	882
HhaI	GCGC	5 cut(s)	261, 263, 437, 571, 827
Hin1II	CATG	5 cut(s)	8, 194, 319, 1667, 2089
Hin6I	GCGC	5 cut(s)	259, 261, 435, 569, 825
HinP1I	GCGC	5 cut(s)	259, 261, 435, 569, 825
HincII	GTYRAC	3 cut(s)	636, 1047, 2372
HindII	GTYRAC	3 cut(s)	636, 1047, 2372
HindIII	AAGCTT	1 cut(s)	2034
HpaII	CCGG	7 cut(s)	199, 442, 611, 1203, 1262, 1551, 2471
HphI	GGTGA	3 cut(s)	2124, 2341, 2753
Hpy99I	CGWCG	1 cut(s)	131
HpyAV	CCTTC	8 cut(s)	196, 303, 992, 1954, 2221, 2264, 2630, 2790
HpyCH4III	ACNGT	9 cut(s)	640, 837, 1210, 1287, 1448, 1651, 1774, 2203, 2440
HpyCH4IV	ACGT	3 cut(s)	69, 1071, 2601
HpyF3I	CTNAG	7 cut(s)	327, 705, 1883, 2061, 2292, 2526, 2669
HpySE526I	ACGT	3 cut(s)	69, 1071, 2601
Hsp92II	CATG	5 cut(s)	8, 194, 319, 1667, 2089
HspAI	GCGC	5 cut(s)	259, 261, 435, 569, 825
Kpn2I	TCCGGA	2 cut(s)	441, 2470
LguI	GCTCTTC	1 cut(s)	1587
LmnI	GCTCC	8 cut(s)	225, 256, 361, 444, 613, 1697, 2101, 2384
Lsp1109I	GCAGC	9 cut(s)	376, 553, 722, 1462, 1687, 1808, 1846, 1931, 2029
MaeI	CTAG	4 cut(s)	864, 1011, 1733, 2412
MaeII	ACGT	3 cut(s)	69, 1071, 2601
MaeIII	GTNAC	3 cut(s)	1123, 2130, 2741
MbiI	CCGCTC	2 cut(s)	247, 2104
MflI	RGATCY	6 cut(s)	526, 764, 847, 1963, 2057, 2698
MhlI	GDGCHC	5 cut(s)	595, 618, 735, 1564, 2662
MlsI	TGGCCA	1 cut(s)	2277
MluI	ACGCGT	1 cut(s)	1054
MluNI	TGGCCA	1 cut(s)	2277
MlyI	GAGTC	6 cut(s)	234, 1124, 1208, 1730, 1830, 2417
MmeI	TCCRAC	5 cut(s)	958, 1092, 1192, 1384, 1933
Mox20I	TGGCCA	1 cut(s)	2277
Mph1103I	ATGCAT	3 cut(s)	6, 1326, 1665
MroI	TCCGGA	2 cut(s)	441, 2470
MroXI	GAANNNNTTC	1 cut(s)	1728
MscI	TGGCCA	1 cut(s)	2277
MseI	TTAA	9 cut(s)	669, 1082, 1133, 1374, 1578, 2123, 2313, 2687, 2854
Msp20I	TGGCCA	1 cut(s)	2277
MspA1I	CMGCKG	1 cut(s)	1622
MspI	CCGG	7 cut(s)	199, 442, 611, 1203, 1262, 1551, 2471
MspR9I	CCNGG	6 cut(s)	419, 425, 1388, 1968, 2139, 2739
Mva1269I	GAATGC	2 cut(s)	2107, 2323
MvaI	CCWGG	6 cut(s)	419, 425, 1388, 1968, 2139, 2739
MvnI	CGCG	5 cut(s)	261, 263, 435, 1056, 1120
NcoI	CCATGG	1 cut(s)	190
NdeI	CATATG	2 cut(s)	2419, 2627
NheI	GCTAGC	1 cut(s)	863
NlaIII	CATG	5 cut(s)	8, 194, 319, 1667, 2089
NlaIV	GGNNCC	4 cut(s)	1782, 1965, 2350, 2777
NmeAIII	GCCGAG	1 cut(s)	379
NmuCI	GTSAC	2 cut(s)	2130, 2741
NsiI	ATGCAT	3 cut(s)	6, 1326, 1665
NspI	RCATGY	1 cut(s)	8
PauI	GCGCGC	1 cut(s)	259
PciSI	GCTCTTC	1 cut(s)	1587
PcsI	WCGNNNNNNNCGW	3 cut(s)	234, 260, 530
PctI	GAATGC	2 cut(s)	2107, 2323
PdmI	GAANNNNTTC	1 cut(s)	1728
PfeI	GAWTC	5 cut(s)	1356, 1405, 2288, 2609, 2711
PleI	GAGTC	6 cut(s)	233, 1123, 1207, 1730, 1829, 2416
PpsI	GAGTC	6 cut(s)	233, 1123, 1207, 1730, 1829, 2416
PpuMI	RGGWCCY	1 cut(s)	2620
PshBI	ATTAAT	4 cut(s)	1082, 1374, 2123, 2687
Psp124BI	GAGCTC	3 cut(s)	595, 735, 1564
Psp5II	RGGWCCY	1 cut(s)	2620
Psp6I	CCWGG	6 cut(s)	417, 423, 1386, 1966, 2137, 2737
PspEI	GGTNACC	1 cut(s)	2130
PspFI	CCCAGC	1 cut(s)	2423
PspGI	CCWGG	6 cut(s)	417, 423, 1386, 1966, 2137, 2737
PspN4I	GGNNCC	4 cut(s)	1782, 1965, 2350, 2777
PspPI	GGNCC	7 cut(s)	421, 427, 1435, 1672, 1781, 2000, 2620
PspPPI	RGGWCCY	1 cut(s)	2620
PstNI	CAGNNNCTG	1 cut(s)	2533
PsuI	RGATCY	6 cut(s)	526, 764, 847, 1963, 2057, 2698
PteI	GCGCGC	1 cut(s)	259
RsaI	GTAC	5 cut(s)	103, 493, 1484, 1648, 2076
RsaNI	GTAC	5 cut(s)	102, 492, 1483, 1647, 2075
SacI	GAGCTC	3 cut(s)	595, 735, 1564
SalI	GTCGAC	1 cut(s)	2370
SapI	GCTCTTC	1 cut(s)	1587
SaqAI	TTAA	9 cut(s)	669, 1082, 1133, 1374, 1578, 2123, 2313, 2687, 2854
Sau96I	GGNCC	7 cut(s)	421, 427, 1435, 1672, 1781, 2000, 2620
ScaI	AGTACT	1 cut(s)	103
SchI	GAGTC	6 cut(s)	234, 1124, 1208, 1730, 1830, 2417
ScrFI	CCNGG	6 cut(s)	419, 425, 1388, 1968, 2139, 2739
SduI	GDGCHC	5 cut(s)	595, 618, 735, 1564, 2662
SinI	GGWCC	5 cut(s)	421, 1672, 1781, 2000, 2620
SmlI	CTYRAG	1 cut(s)	2453
SmoI	CTYRAG	1 cut(s)	2453
SpeI	ACTAGT	1 cut(s)	1010
SspI	AATATT	1 cut(s)	1252
SspMI	CTAG	4 cut(s)	864, 1011, 1733, 2412
SstI	GAGCTC	3 cut(s)	595, 735, 1564
StyD4I	CCNGG	6 cut(s)	417, 423, 1386, 1966, 2137, 2737
StyI	CCWWGG	3 cut(s)	190, 297, 1065
TaaI	ACNGT	9 cut(s)	640, 837, 1210, 1287, 1448, 1651, 1774, 2203, 2440
TaiI	ACGT	3 cut(s)	72, 1074, 2604
TaqI	TCGA	8 cut(s)	46, 215, 243, 581, 769, 1533, 2223, 2371
TaqII	GACCGA	2 cut(s)	953, 1866
TatI	WGTACW	1 cut(s)	101
TauI	GCSGC	4 cut(s)	198, 435, 457, 2107
TfiI	GAWTC	5 cut(s)	1356, 1405, 2288, 2609, 2711
Tru1I	TTAA	9 cut(s)	669, 1082, 1133, 1374, 1578, 2123, 2313, 2687, 2854
Tru9I	TTAA	9 cut(s)	669, 1082, 1133, 1374, 1578, 2123, 2313, 2687, 2854
TscAI	CASTG	5 cut(s)	859, 1292, 1726, 1846, 2752
TseFI	GTSAC	2 cut(s)	2130, 2741
TseI	GCWGC	9 cut(s)	364, 566, 710, 1475, 1700, 1796, 1834, 1919, 2042
Tsp45I	GTSAC	2 cut(s)	2130, 2741
TspGWI	ACGGA	5 cut(s)	13, 269, 539, 1729, 2314
TspRI	CASTG	5 cut(s)	859, 1292, 1726, 1846, 2752
VpaK11BI	GGWCC	5 cut(s)	421, 1672, 1781, 2000, 2620
VspI	ATTAAT	4 cut(s)	1082, 1374, 2123, 2687
XagI	CCTNNNNNAGG	2 cut(s)	422, 1544
XapI	RAATTY	2 cut(s)	385, 1641
XbaI	TCTAGA	1 cut(s)	1732
XceI	RCATGY	1 cut(s)	8
XmiI	GTMKAC	3 cut(s)	1972, 2371, 2833
XmnI	GAANNNNTTC	1 cut(s)	1728
XspI	CTAG	4 cut(s)	864, 1011, 1733, 2412
ZrmI	AGTACT	1 cut(s)	103
Zsp2I	ATGCAT	3 cut(s)	6, 1326, 1665

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.