Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0013822

Physical Location & Seq

Genomic Coordinates Forward (+)

27077484 .. 27083177

Gene Structure 5694 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1128400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2904 bp

ATGCAAGTGTCATCGTCGTCGTCCTCCTCCGTGCTGATTCCTTCACTTCAATGGTGGACTCCGTCTCTAAATTCTTCTACTTCTAATTGTTGCTGCACTCAATTCCTCCGTTATGATCGCAGCCGAAGCAGAAGTAGCGGCGGCGGCAGGCAAGTCTTCTCTCTCTACCCAATTCGCCTCGCCAACCCATCACCCCCTTTTGCTTCTTCTTTCCTTTCCCCCTTCAAAGCTCGCACCTTTCCCACCGGCCCCACCGGCCGGCGAAAAACCAAGCTCCGGATCGTCGCCGCCGTCTTCGAGCGGTTTACGGAGCGAGCAGTCAAATCCGTCATCTTTTCCCAAAGAGAAGCCAGAGCTCTCGGCAGAAACATGGTGTTTACCCAGCACCTGCTTCTGGGTCTGATCGCCGAGGAGGAGCAGCACCGCCATCTTCATCCGAATTCCCACGGCTTTCTGGGCTCGGGCATCTCTATAAAGCAGGCCCGGGAAGCTGTTCGCGCCATTTGGCACCACCATAGTCAAAGTCAGACTTCTGCTTCTGCAGAAGTTGATCCTTCTAGCCGAGCTGCTTCCTCTTCCGCCACTGATGTCCCCTTTTCAATTAGCACAAAGCGGGTCCTCGAGGCGGCGTTGGAGTATTCGAGAGCTAGAGCTCACAACTTCATTGCGCCGGAGCACATTACCATTGGTTTATTCACCGCTGATGATGGAAGTGCTGGTCAGGTCCTCAAAAGACTGGGGGTAGATGTAAACCAGTTGTTAGCCGAGGCAGCCTCGAGGCTTCAAGTAGAGCTTGTCAAAGATGGTAGAGAACCATCTGGGGGATTTCAAAAATCCCTTTCCAAAAAATCAACTGCTTCTGGGAAAAAGAAAGATAAAAGTGCTTTGGACCAATTCTGCGTGGATCTTACTGCTTGTGCTAGTGAGGGACTCATTGATCCTGTTATTGGCCGAGACACTGAAGTTCAGAGAATTATTCAGATACTTTGTCGCAGAAGTAAAAATAACCCCATTCTTCTTGGTGAAAGTGGGGTTGGAAAGACGGCCATTGTTGAAGGACTGGCGATTAGTATTGCACAGGCGGATGTCCCTGCTTTTCTCCTGGAAAAACGTGTAATGTCCTTGGATATAGCACTATTAATGGCTGGTTCAAAAGAGAGGGGGGAAATGGAGGCGCGTGTTACCACATTGTTAAGTGACATAAGAAAAGCAGGAAACATTATTCTTTTTATTGATGAAGTACATACCCTCATTGAGTCTGGAACAGTTGGACAAAGAAATAAGGGGTCTAGTCTTGACATTGCCAATCTAATAAAACCGCCCCTTGGGAGGGGTCAATTACAGTGTATTGCAGCCACAACTGCAGATGAATACAGGTTGCACCTTGAAAAGGATAAAGCATTTGCACGAAGATTACAACCTGTTTGGATTAATGAACCAAGCCAGGATGATGCAGTTAGGATTCTATTAGGTCTACGTGAGAAATATGAGGCCCATCACAACTGCAGATATGAACCAGAAGCCATTATTGCAGCTGTGTATCTAGCAGCAAGATATATACCTGATAGATATCTACCTGATAAAGCTATTGATCTCATTGATGAAGCGGGAAGTAGAGCTCGTATGGATGCCTTTAAGAGGAAAAGAGAACAGCAAATTTGTATACTTTCAAAGTCACCTGATGACTATTGGCAAGAAATTCGGACAGTTCAGGCGATGCATGAAGTGGTCCTAGCGACTGAACTAAAAAATAGCGATGCTTCTAGTGTGGATGATATCAGAGAATCCTCCTCTTCATCTTCCACATCTGGTGATGAACCTGCAGTGGTGGGACCAGAGGATATAGCAGCAGTTGCCTCACTATGGTCAGGAGTCCCTCTTCAGCAGCTCACCGCTGATGACAGAATGCTTCTGGTCGGTCTTGATGAGAAGCTTAGGAAACGGGTTGTTGGTCAAGAAGAGGCTGTTGCTGCCATTTCTCGAGCTGTTAAGAGATCCCGGGTTGGACTGAAGGATCCTGACAGACCCATGGCAACACTGCTCTTTTGTGGTCCGACCGGGGTTGGCAAAACTGAATTAACAAAAGCTTTGGCTGCATCTTATTTTGGATCAGAGGAAGCCATGCTACGGTTTGACATGAGTGAATATATGGAGCGCCATTCTGTTAGCAAATTAATAGGGTCACCCCCAGGTTATGTTGGCTATGGAGCTGGGGGCACTCTAACCGAAGCTATTAGAAGACGACCTTTTACAGTGGTTGTGTTTGATGAAATAGAGAAAGCCCATCCAGATATCTTCAACATCCTTCTCCAAATTTTTGAAGATGGCCACCTGACTGATGCTCAGGGAAGGAGAGTGTCATTTAAGAATGCGTTGGTAGTGATGACGTCTAATGTTGGGTCCACTATCATTGCTAAGGGCAGACAAAGCTCCATTGGTTTCTTGCTTGCAGATGATGAGGTAACTTCATACGCCGGACTAAAAGCAACTGTAATGGAAGAACTCAAGACATATTTTCGTCCAGAGTTGCTGAATCGGATAGACGAAGTAGTTGTATTCCATCCCCTTCAAAAGGCTCAGATGCTCGAGATTGTAAACTTAATGCTACAAGAGCTGAAGAAGAGGCTGATGTCTCTGGGAATGGGATTGGAGGTGTCTGAATCTGTCAAGGGCCTCATATGTGAGCAAGGCTACGACCGGTTTTATGGCGCCCGCCCGCTTAGGAGGGCGATTACGTCAATAATTGAAGATCCGTTGAGCGAAGCGCTTCTTGCCGGAGGTTACAAGCCCGGTGACACTGTCGTTATCGACGTAGATGCTACCGGAAACCCGTTCATAAGAAATGGATCAGATCAGAGTGTACGGTTGTCCAACAGCAGTATACAACATCCATCTTGTAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009941 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022607 GO:0031967 GO:0031975 GO:0033554 GO:0034214 GO:0034605 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046983 GO:0047484 GO:0048518 GO:0048583 GO:0048584 GO:0050789 GO:0050896 GO:0051259 GO:0051716 GO:0065003 GO:0065007 GO:0071840 GO:0080134 GO:1901000 GO:1901002 GO:1902584 GO:2000070

KEGG Pathways

Metabolic & Signaling

ko01100 map01100

Pfam Domains

Protein Families

AAA AAA_2 ClpB_D2-small Clp_N

Protein Analysis

968

Amino Acids

106.06

Weight (kDa)

8.41

Isoelectric Point (pI)

49.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	199 - 281	3.2e-10	Clp repeat (R) N-terminal domain
NBD_SMAX1	PF23569	321 - 429	6.5e-06	SMAX1 nucleotide binding domain
AAA	PF00004	338 - 440	1.2e-08	ATPase family associated with various cellular activities (AAA)
AAA_lid_9	PF17871	481 - 560	4.3e-26	AAA lid domain
AAA_2	PF07724	675 - 850	1.2e-62	AAA domain (Cdc48 subfamily)
AAA_5	PF07728	680 - 800	5.1e-10	AAA domain (dynein-related subfamily)
ClpB_D2-small	PF10431	856 - 936	4.7e-24	C-terminal, D2-small domain, of ClpB protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0013822)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51070
fragaria_vesca	FvH4_5g10020
malus_domestica	MD06G1128400.v1.1
prunus_persica	Prupe.5G138200_v2.0.a1
pyrus_communis	pycom06g11860
rosa_chinensis	RchiOBHm_Chr7g0188241
rosa_laevigata	RLG00000004715
rosa_multiflora	Rmu_co8357845.1_g000001 Rmu_co8388145.1_g000001 Rmu_sc0008750.1_g000013
rosa_roxburghii	Rroxscaffold_3G00266580
rosa_rugosa	Rorug06G0489400
rosa_samantha	Rh7AG093700 Rh7BG095100 Rh7CG095500 Rh7DG095700
rosa_wichuraiana	Rw7G008070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	396
AatII	GACGTC	1 cut(s)	2390
AbsI	CCTCGAGG	2 cut(s)	620, 775
Acc36I	ACCTGC	2 cut(s)	396, 1828
AccB1I	GGYRCC	2 cut(s)	507, 2708
AccBSI	CCGCTC	1 cut(s)	301
AccI	GTMKAC	3 cut(s)	1474, 1663, 2881
AccII	CGCG	2 cut(s)	498, 1177
AccIII	TCCGGA	1 cut(s)	276
AcoI	YGGCCR	4 cut(s)	256, 949, 1044, 2326
AcsI	RAATTY	5 cut(s)	70, 439, 1656, 1698, 2313
AcuI	CTGAAG	4 cut(s)	981, 1865, 2030, 2636
AcyI	GRCGYC	2 cut(s)	2387, 2709
AfaI	GTAC	2 cut(s)	1242, 2862
AfeI	AGCGCT	1 cut(s)	2766
AflIII	ACRYGT	1 cut(s)	1111
AgeI	ACCGGT	1 cut(s)	2697
AjnI	CCWGG	3 cut(s)	1101, 1443, 2188
AjuI	GAANNNNNNNTTGG	2 cut(s)	1017, 1049
Alw21I	GWGCWC	4 cut(s)	358, 655, 678, 1621
Alw26I	GTCTC	3 cut(s)	69, 948, 2637
AlwNI	CAGNNNCTG	1 cut(s)	388
Ama87I	CYCGRG	7 cut(s)	460, 483, 620, 775, 1980, 1998, 2585
Aor13HI	TCCGGA	1 cut(s)	276
Aor51HI	AGCGCT	1 cut(s)	2766
AoxI	GGCC	8 cut(s)	247, 256, 480, 949, 1044, 1491, 2326, 2671
ApoI	RAATTY	5 cut(s)	70, 439, 1656, 1698, 2313
AseI	ATTAAT	3 cut(s)	1139, 1431, 2174
AsiGI	ACCGGT	1 cut(s)	2697
Asp700I	GAANNNNTTC	2 cut(s)	492, 2766
AspLEI	GCGC	6 cut(s)	500, 670, 1177, 2157, 2711, 2767
AsuC2I	CCSGG	6 cut(s)	484, 485, 1999, 2000, 2059, 2790
AsuHPI	GGTGA	8 cut(s)	183, 688, 1034, 1668, 1823, 1882, 2175, 2804
AvaI	CYCGRG	7 cut(s)	460, 483, 620, 775, 1980, 1998, 2585
AvaII	GGWCC	7 cut(s)	616, 724, 889, 1729, 1832, 2051, 2400
BaeGI	GKGCMC	1 cut(s)	2219
BalI	TGGCCA	1 cut(s)	2328
BamHI	GGATCC	1 cut(s)	2014
BanI	GGYRCC	2 cut(s)	507, 2708
BanII	GRGCYC	4 cut(s)	358, 461, 655, 1621
BarI	GAAGNNNNNNTAC	2 cut(s)	2109, 2141
BbsI	GAAGAC	3 cut(s)	148, 286, 2245
Bbv12I	GWGCWC	4 cut(s)	358, 655, 678, 1621
BceAI	ACGGC	3 cut(s)	275, 463, 1059
BcgI	CGANNNNNNTGC	4 cut(s)	1682, 1716, 2798, 2832
BciT130I	CCWGG	3 cut(s)	1103, 1445, 2190
BcnI	CCSGG	6 cut(s)	484, 485, 1999, 2000, 2059, 2790
BcoDI	GTCTC	3 cut(s)	69, 948, 2637
BfaI	CTAG	7 cut(s)	558, 648, 921, 1290, 1544, 1733, 1764
BfmI	CTRYAG	4 cut(s)	540, 1362, 1504, 1821
BfoI	RGCGCY	3 cut(s)	2158, 2712, 2768
BfuAI	ACCTGC	2 cut(s)	396, 1828
BglI	GCCNNNNNGGC	1 cut(s)	255
Bme1390I	CCNGG	9 cut(s)	484, 485, 1103, 1445, 1999, 2000, 2059, 2190, 2790
Bme18I	GGWCC	7 cut(s)	616, 724, 889, 1729, 1832, 2051, 2400
BmeT110I	CYCGRG	7 cut(s)	460, 483, 620, 775, 1980, 1998, 2585
BmiI	GGNNCC	7 cut(s)	250, 509, 617, 1833, 2016, 2401, 2710
BmrFI	CCNGG	9 cut(s)	484, 485, 1103, 1445, 1999, 2000, 2059, 2190, 2790
BmrI	ACTGGG	1 cut(s)	746
BmsI	GCATC	9 cut(s)	474, 1441, 1618, 1707, 1747, 2105, 2329, 2571, 2806
BmuI	ACTGGG	1 cut(s)	746
BpiI	GAAGAC	3 cut(s)	148, 286, 2245
BplI	GAGNNNNNCTC	2 cut(s)	758, 790
Bpu10I	CCTNAGC	4 cut(s)	1934, 2343, 2415, 2720
BpuEI	CTTGAG	1 cut(s)	2489
BpuMI	CCSGG	6 cut(s)	484, 485, 1999, 2000, 2059, 2790
BsaAI	YACGTR	1 cut(s)	1478
BsaBI	GATNNNNATC	1 cut(s)	1569
BsaHI	GRCGYC	2 cut(s)	2387, 2709
BsaWI	WCCGGW	3 cut(s)	276, 2697, 2822
Bse118I	RCCGGY	4 cut(s)	245, 254, 258, 2697
Bse1I	ACTGG	3 cut(s)	741, 754, 1065
Bse3DI	GCAATG	3 cut(s)	663, 1299, 2409
Bse8I	GATNNNNATC	1 cut(s)	1569
BseAI	TCCGGA	1 cut(s)	276
BseBI	CCWGG	3 cut(s)	1103, 1445, 2190
BseGI	GGATG	9 cut(s)	433, 1090, 1453, 1633, 1777, 2284, 2301, 2560, 2887
BseJI	GATNNNNATC	1 cut(s)	1569
BseMI	GCAATG	3 cut(s)	663, 1299, 2409
BseMII	CTCAG	2 cut(s)	2357, 2591
BseNI	ACTGG	3 cut(s)	741, 754, 1065
BseRI	GAGGAG	4 cut(s)	16, 425, 428, 1780
BseSI	GKGCMC	1 cut(s)	2219
BseX3I	CGGCCG	1 cut(s)	256
BseYI	CCCAGC	2 cut(s)	381, 2210
BsgI	GTGCAG	1 cut(s)	79
Bsh1236I	CGCG	2 cut(s)	498, 1177
Bsh1285I	CGRYCG	3 cut(s)	259, 2058, 2698
BshFI	GGCC	8 cut(s)	249, 258, 482, 951, 1046, 1493, 2328, 2673
BshNI	GGYRCC	2 cut(s)	507, 2708
BshTI	ACCGGT	1 cut(s)	2697
BsiEI	CGRYCG	3 cut(s)	259, 2058, 2698
BsiHKAI	GWGCWC	4 cut(s)	358, 655, 678, 1621
BsiHKCI	CYCGRG	7 cut(s)	460, 483, 620, 775, 1980, 1998, 2585
BslFI	GGGAC	5 cut(s)	575, 942, 1073, 1845, 1859
BsmAI	GTCTC	3 cut(s)	69, 948, 2637
BsmBI	CGTCTC	1 cut(s)	69
BsmFI	GGGAC	5 cut(s)	575, 942, 1073, 1845, 1859
BsmI	GAATGC	2 cut(s)	1911, 2374
BsnI	GGCC	8 cut(s)	249, 258, 482, 951, 1046, 1493, 2328, 2673
BsoBI	CYCGRG	7 cut(s)	460, 483, 620, 775, 1980, 1998, 2585
Bsp1286I	GDGCHC	6 cut(s)	358, 461, 655, 678, 1621, 2219
Bsp13I	TCCGGA	1 cut(s)	276
Bsp19I	CCATGG	1 cut(s)	2028
BspANI	GGCC	8 cut(s)	249, 258, 482, 951, 1046, 1493, 2328, 2673
BspCNI	CTCAG	2 cut(s)	2356, 2590
BspEI	TCCGGA	1 cut(s)	276
BspFNI	CGCG	2 cut(s)	498, 1177
BspLI	GGNNCC	7 cut(s)	250, 509, 617, 1833, 2016, 2401, 2710
BspMAI	CTGCAG	4 cut(s)	544, 1366, 1508, 1825
BspMI	ACCTGC	2 cut(s)	396, 1828
BspT107I	GGYRCC	2 cut(s)	507, 2708
BsrBI	CCGCTC	1 cut(s)	301
BsrDI	GCAATG	3 cut(s)	663, 1299, 2409
BsrFI	RCCGGY	4 cut(s)	245, 254, 258, 2697
BsrI	ACTGG	3 cut(s)	741, 754, 1065
BssAI	RCCGGY	4 cut(s)	245, 254, 258, 2697
BssNAI	GTATAC	2 cut(s)	1664, 2882
BssNI	GRCGYC	2 cut(s)	2387, 2709
BssT1I	CCWWGG	3 cut(s)	1122, 1324, 2028
Bst1107I	GTATAC	2 cut(s)	1664, 2882
Bst2UI	CCWGG	3 cut(s)	1103, 1445, 2190
Bst4CI	ACNGT	8 cut(s)	1267, 1344, 1708, 2130, 2254, 2491, 2800, 2865
Bst6I	CTCTTC	5 cut(s)	580, 1798, 1884, 1953, 2615
BstACI	GRCGYC	2 cut(s)	2387, 2709
BstAPI	GCANNNNNTGC	1 cut(s)	1853
BstBAI	YACGTR	1 cut(s)	1478
BstC8I	GCNNGC	8 cut(s)	149, 232, 260, 315, 480, 2448, 2713, 2717
BstDEI	CTNAG	5 cut(s)	1934, 2343, 2415, 2577, 2720
BstDSI	CCRYGG	2 cut(s)	445, 2028
BstEII	GGTNACC	1 cut(s)	2181
BstENI	CCTNNNNNAGG	3 cut(s)	1388, 1636, 2570
BstF5I	GGATG	9 cut(s)	433, 1090, 1453, 1633, 1777, 2284, 2301, 2560, 2887
BstFNI	CGCG	2 cut(s)	498, 1177
BstH2I	RGCGCY	3 cut(s)	2158, 2712, 2768
BstHHI	GCGC	6 cut(s)	500, 670, 1177, 2157, 2711, 2767
BstMAI	GTCTC	3 cut(s)	69, 948, 2637
BstMCI	CGRYCG	3 cut(s)	259, 2058, 2698
BstNI	CCWGG	3 cut(s)	1103, 1445, 2190
BstPI	GGTNACC	1 cut(s)	2181
BstSCI	CCNGG	9 cut(s)	482, 483, 1101, 1443, 1997, 1998, 2057, 2188, 2788
BstSFI	CTRYAG	4 cut(s)	540, 1362, 1504, 1821
BstSLI	GKGCMC	1 cut(s)	2219
BstUI	CGCG	2 cut(s)	498, 1177
BstV2I	GAAGAC	3 cut(s)	148, 286, 2245
BstX2I	RGATCY	4 cut(s)	904, 1994, 2014, 2749
BstYI	RGATCY	4 cut(s)	904, 1994, 2014, 2749
BstZ17I	GTATAC	2 cut(s)	1664, 2882
BstZI	CGGCCG	1 cut(s)	256
BsuRI	GGCC	8 cut(s)	249, 258, 482, 951, 1046, 1493, 2328, 2673
BtgI	CCRYGG	2 cut(s)	445, 2028
BtgZI	GCGATG	2 cut(s)	1730, 1770
BtsCI	GGATG	9 cut(s)	433, 1090, 1453, 1633, 1777, 2284, 2301, 2560, 2887
BtsI	GCAGTG	2 cut(s)	1830, 2036
BtsIMutI	CAGTG	7 cut(s)	582, 957, 1349, 1830, 2036, 2259, 2796
BveI	ACCTGC	2 cut(s)	396, 1828
Cac8I	GCNNGC	8 cut(s)	149, 232, 260, 315, 480, 2448, 2713, 2717
CaiI	CAGNNNCTG	1 cut(s)	388
CfoI	GCGC	6 cut(s)	500, 670, 1177, 2157, 2711, 2767
Cfr10I	RCCGGY	4 cut(s)	245, 254, 258, 2697
Cfr9I	CCCGGG	2 cut(s)	483, 1998
Csp6I	GTAC	2 cut(s)	1241, 2861
CspAI	ACCGGT	1 cut(s)	2697
CspCI	CAANNNNNGTGG	2 cut(s)	2392, 2427
CviAII	CATG	5 cut(s)	370, 1721, 2029, 2122, 2137
CviQI	GTAC	2 cut(s)	1241, 2861
DdeI	CTNAG	5 cut(s)	1934, 2343, 2415, 2577, 2720
DinI	GGCGCC	1 cut(s)	2710
EaeI	YGGCCR	4 cut(s)	256, 949, 1044, 2326
EagI	CGGCCG	1 cut(s)	256
Eam1104I	CTCTTC	5 cut(s)	580, 1798, 1884, 1953, 2615
EarI	CTCTTC	5 cut(s)	580, 1798, 1884, 1953, 2615
EciI	GGCGGA	2 cut(s)	568, 1097
Ecl136II	GAGCTC	3 cut(s)	356, 653, 1619
EclXI	CGGCCG	1 cut(s)	256
Eco130I	CCWWGG	3 cut(s)	1122, 1324, 2028
Eco24I	GRGCYC	4 cut(s)	358, 461, 655, 1621
Eco32I	GATATC	3 cut(s)	1571, 1777, 2293
Eco47I	GGWCC	7 cut(s)	616, 724, 889, 1729, 1832, 2051, 2400
Eco47III	AGCGCT	1 cut(s)	2766
Eco52I	CGGCCG	1 cut(s)	256
Eco53kI	GAGCTC	3 cut(s)	356, 653, 1619
Eco57I	CTGAAG	4 cut(s)	981, 1865, 2030, 2636
Eco88I	CYCGRG	7 cut(s)	460, 483, 620, 775, 1980, 1998, 2585
Eco91I	GGTNACC	1 cut(s)	2181
EcoICRI	GAGCTC	3 cut(s)	356, 653, 1619
EcoNI	CCTNNNNNAGG	3 cut(s)	1388, 1636, 2570
EcoO109I	RGGNCCY	3 cut(s)	616, 724, 2671
EcoO65I	GGTNACC	1 cut(s)	2181
EcoRI	GAATTC	1 cut(s)	439
EcoRII	CCWGG	3 cut(s)	1101, 1443, 2188
EcoRV	GATATC	3 cut(s)	1571, 1777, 2293
EcoT14I	CCWWGG	3 cut(s)	1122, 1324, 2028
EcoT22I	ATGCAT	1 cut(s)	1722
EcoT38I	GRGCYC	4 cut(s)	358, 461, 655, 1621
EgeI	GGCGCC	1 cut(s)	2710
EheI	GGCGCC	1 cut(s)	2710
ErhI	CCWWGG	3 cut(s)	1122, 1324, 2028
Esp3I	CGTCTC	1 cut(s)	69
FaeI	CATG	5 cut(s)	373, 1724, 2032, 2125, 2140
FalI	AAGNNNNNCTT	8 cut(s)	514, 546, 777, 809, 2230, 2262, 2754, 2786
FaqI	GGGAC	5 cut(s)	575, 942, 1073, 1845, 1859
FatI	CATG	5 cut(s)	369, 1720, 2028, 2121, 2136
FauI	CCCGC	4 cut(s)	606, 1600, 2720, 2724
FauNDI	CATATG	1 cut(s)	2678
FblI	GTMKAC	3 cut(s)	1474, 1663, 2881
FokI	GGATG	9 cut(s)	420, 1097, 1460, 1640, 1784, 2271, 2288, 2547, 2874
FriOI	GRGCYC	4 cut(s)	358, 461, 655, 1621
FspBI	CTAG	7 cut(s)	558, 648, 921, 1290, 1544, 1733, 1764
GlaI	GCGC	6 cut(s)	499, 669, 1176, 2156, 2710, 2766
GsaI	CCCAGC	2 cut(s)	385, 2214
HaeII	RGCGCY	3 cut(s)	2158, 2712, 2768
HaeIII	GGCC	8 cut(s)	249, 258, 482, 951, 1046, 1493, 2328, 2673
HhaI	GCGC	6 cut(s)	500, 670, 1177, 2157, 2711, 2767
Hin1I	GRCGYC	2 cut(s)	2387, 2709
Hin1II	CATG	5 cut(s)	373, 1724, 2032, 2125, 2140
Hin6I	GCGC	6 cut(s)	498, 668, 1175, 2155, 2709, 2765
HinP1I	GCGC	6 cut(s)	498, 668, 1175, 2155, 2709, 2765
HindIII	AAGCTT	2 cut(s)	1931, 2085
HinfI	GANTC	9 cut(s)	37, 58, 930, 1256, 1462, 1784, 1872, 2533, 2660
HphI	GGTGA	8 cut(s)	183, 688, 1034, 1668, 1823, 1882, 2175, 2804
Hpy99I	CGWCG	4 cut(s)	19, 22, 287, 2813
HpyAV	CCTTC	8 cut(s)	51, 232, 564, 1049, 2005, 2315, 2343, 2576
HpyCH4III	ACNGT	8 cut(s)	1267, 1344, 1708, 2130, 2254, 2491, 2800, 2865
HpyCH4IV	ACGT	5 cut(s)	1111, 1477, 2387, 2735, 2811
HpyF3I	CTNAG	5 cut(s)	1934, 2343, 2415, 2577, 2720
HpySE526I	ACGT	5 cut(s)	1111, 1477, 2387, 2735, 2811
Hsp92I	GRCGYC	2 cut(s)	2387, 2709
Hsp92II	CATG	5 cut(s)	373, 1724, 2032, 2125, 2140
HspAI	GCGC	6 cut(s)	498, 668, 1175, 2155, 2709, 2765
KasI	GGCGCC	1 cut(s)	2708
Kpn2I	TCCGGA	1 cut(s)	276
KroI	GCCGGC	1 cut(s)	258
KroNI	GCCGGC	1 cut(s)	260
LmnI	GCTCC	7 cut(s)	279, 310, 415, 673, 2152, 2207, 2435
LweI	GCATC	9 cut(s)	474, 1441, 1618, 1707, 1747, 2105, 2329, 2571, 2806
MaeI	CTAG	7 cut(s)	558, 648, 921, 1290, 1544, 1733, 1764
MaeII	ACGT	5 cut(s)	1111, 1477, 2387, 2735, 2811
MaeIII	GTNAC	7 cut(s)	1180, 1196, 1674, 2181, 2461, 2780, 2792
MbiI	CCGCTC	1 cut(s)	301
MflI	RGATCY	4 cut(s)	904, 1994, 2014, 2749
MhlI	GDGCHC	6 cut(s)	358, 461, 655, 678, 1621, 2219
MlsI	TGGCCA	1 cut(s)	2328
MluNI	TGGCCA	1 cut(s)	2328
Mly113I	GGCGCC	1 cut(s)	2709
MlyI	GAGTC	4 cut(s)	52, 924, 1265, 1881
MmeI	TCCRAC	6 cut(s)	612, 1015, 1249, 1984, 2078, 2895
Mox20I	TGGCCA	1 cut(s)	2328
Mph1103I	ATGCAT	1 cut(s)	1722
MroI	TCCGGA	1 cut(s)	276
MroNI	GCCGGC	1 cut(s)	258
MroXI	GAANNNNTTC	2 cut(s)	492, 2766
MscI	TGGCCA	1 cut(s)	2328
MslI	CAYNNNNRTG	1 cut(s)	49
Msp20I	TGGCCA	1 cut(s)	2328
MspA1I	CMGCKG	3 cut(s)	701, 1535, 1895
MspR9I	CCNGG	9 cut(s)	484, 485, 1103, 1445, 1999, 2000, 2059, 2190, 2790
Mva1269I	GAATGC	2 cut(s)	1911, 2374
MvaI	CCWGG	3 cut(s)	1103, 1445, 2190
MvnI	CGCG	2 cut(s)	498, 1177
NaeI	GCCGGC	1 cut(s)	260
NarI	GGCGCC	1 cut(s)	2709
NciI	CCSGG	6 cut(s)	484, 485, 1999, 2000, 2059, 2790
NcoI	CCATGG	1 cut(s)	2028
NdeI	CATATG	1 cut(s)	2678
NgoMIV	GCCGGC	1 cut(s)	258
NlaIII	CATG	5 cut(s)	373, 1724, 2032, 2125, 2140
NlaIV	GGNNCC	7 cut(s)	250, 509, 617, 1833, 2016, 2401, 2710
NmeAIII	GCCGAG	5 cut(s)	339, 433, 587, 790, 977
NmuCI	GTSAC	4 cut(s)	1196, 1674, 2181, 2792
NsiI	ATGCAT	1 cut(s)	1722
PaeR7I	CTCGAG	4 cut(s)	620, 775, 1980, 2585
PaqCI	CACCTGC	1 cut(s)	396
PcsI	WCGNNNNNNNCGW	2 cut(s)	288, 2808
PctI	GAATGC	2 cut(s)	1911, 2374
PdiI	GCCGGC	1 cut(s)	260
PdmI	GAANNNNTTC	2 cut(s)	492, 2766
PfeI	GAWTC	5 cut(s)	37, 1462, 1784, 2533, 2660
PflFI	GACNNNGTC	2 cut(s)	61, 2798
PfoI	TCCNGGA	1 cut(s)	1101
PinAI	ACCGGT	1 cut(s)	2697
PleI	GAGTC	4 cut(s)	52, 924, 1264, 1880
PluTI	GGCGCC	1 cut(s)	2712
PpsI	GAGTC	4 cut(s)	52, 924, 1264, 1880
Ppu21I	YACGTR	1 cut(s)	1478
PpuMI	RGGWCCY	2 cut(s)	616, 724
PshBI	ATTAAT	3 cut(s)	1139, 1431, 2174
Psp124BI	GAGCTC	3 cut(s)	358, 655, 1621
Psp5II	RGGWCCY	2 cut(s)	616, 724
Psp6I	CCWGG	3 cut(s)	1101, 1443, 2188
PspEI	GGTNACC	1 cut(s)	2181
PspFI	CCCAGC	2 cut(s)	381, 2210
PspGI	CCWGG	3 cut(s)	1101, 1443, 2188
PspN4I	GGNNCC	7 cut(s)	250, 509, 617, 1833, 2016, 2401, 2710
PspPPI	RGGWCCY	2 cut(s)	616, 724
PspXI	VCTCGAGB	2 cut(s)	620, 775
PstI	CTGCAG	4 cut(s)	544, 1366, 1508, 1825
PstNI	CAGNNNCTG	1 cut(s)	388
PsuI	RGATCY	4 cut(s)	904, 1994, 2014, 2749
PsyI	GACNNNGTC	2 cut(s)	61, 2798
PvuII	CAGCTG	1 cut(s)	1535
RsaI	GTAC	2 cut(s)	1242, 2862
RsaNI	GTAC	2 cut(s)	1241, 2861
RseI	CAYNNNNRTG	1 cut(s)	49
SacI	GAGCTC	3 cut(s)	358, 655, 1621
SchI	GAGTC	4 cut(s)	52, 924, 1265, 1881
ScrFI	CCNGG	9 cut(s)	484, 485, 1103, 1445, 1999, 2000, 2059, 2190, 2790
SduI	GDGCHC	6 cut(s)	358, 461, 655, 678, 1621, 2219
SfaNI	GCATC	9 cut(s)	474, 1441, 1618, 1707, 1747, 2105, 2329, 2571, 2806
SfcI	CTRYAG	4 cut(s)	540, 1362, 1504, 1821
SfiI	GGCCNNNNNGGCC	1 cut(s)	255
SfoI	GGCGCC	1 cut(s)	2710
Sfr274I	CTCGAG	4 cut(s)	620, 775, 1980, 2585
SinI	GGWCC	7 cut(s)	616, 724, 889, 1729, 1832, 2051, 2400
SlaI	CTCGAG	4 cut(s)	620, 775, 1980, 2585
SmaI	CCCGGG	2 cut(s)	485, 2000
SmiMI	CAYNNNNRTG	1 cut(s)	49
SmlI	CTYRAG	5 cut(s)	620, 775, 1980, 2504, 2585
SmoI	CTYRAG	5 cut(s)	620, 775, 1980, 2504, 2585
SspDI	GGCGCC	1 cut(s)	2708
SspMI	CTAG	7 cut(s)	558, 648, 921, 1290, 1544, 1733, 1764
SstI	GAGCTC	3 cut(s)	358, 655, 1621
StyD4I	CCNGG	9 cut(s)	482, 483, 1101, 1443, 1997, 1998, 2057, 2188, 2788
StyI	CCWWGG	3 cut(s)	1122, 1324, 2028
TaaI	ACNGT	8 cut(s)	1267, 1344, 1708, 2130, 2254, 2491, 2800, 2865
TaiI	ACGT	5 cut(s)	1114, 1480, 2390, 2738, 2814
TaqI	TCGA	7 cut(s)	297, 621, 641, 776, 1981, 2586, 2808
TaqII	GACCGA	1 cut(s)	1907
TatI	WGTACW	1 cut(s)	1240
TauI	GCSGC	5 cut(s)	141, 144, 147, 290, 629
TfiI	GAWTC	5 cut(s)	37, 1462, 1784, 2533, 2660
TscAI	CASTG	7 cut(s)	589, 964, 1349, 1830, 2043, 2259, 2803
TseFI	GTSAC	4 cut(s)	1196, 1674, 2181, 2792
Tsp45I	GTSAC	4 cut(s)	1196, 1674, 2181, 2792
TspGWI	ACGGA	6 cut(s)	19, 51, 98, 316, 323, 2742
TspMI	CCCGGG	2 cut(s)	483, 1998
TspRI	CASTG	7 cut(s)	589, 964, 1349, 1830, 2043, 2259, 2803
Tth111I	GACNNNGTC	2 cut(s)	61, 2798
VpaK11BI	GGWCC	7 cut(s)	616, 724, 889, 1729, 1832, 2051, 2400
VspI	ATTAAT	3 cut(s)	1139, 1431, 2174
XagI	CCTNNNNNAGG	3 cut(s)	1388, 1636, 2570
XapI	RAATTY	5 cut(s)	70, 439, 1656, 1698, 2313
XhoI	CTCGAG	4 cut(s)	620, 775, 1980, 2585
XmaI	CCCGGG	2 cut(s)	483, 1998
XmiI	GTMKAC	3 cut(s)	1474, 1663, 2881
XmnI	GAANNNNTTC	2 cut(s)	492, 2766
XspI	CTAG	7 cut(s)	558, 648, 921, 1290, 1544, 1733, 1764
ZraI	GACGTC	1 cut(s)	2388
Zsp2I	ATGCAT	1 cut(s)	1722

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.