Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0013822

Physical Location & Seq

Genomic Coordinates Forward (+)

7862405 .. 7867725

Gene Structure 5321 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ16809

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2904 bp

ATGCATGTGTCGTCATCCTCCTCCGTGCTGATATCATCTCCACTCCAATGGAGAACTCCATCTCAACGCGTATCATTCACTCATCTTCCCTTACAATCGGTTACGACTTGCACTACTATCAGTTACGCCCGTGATCGCAGAAACGCCGTCGTTTCCCTCTACCCAATTCGCCTTATACACAACCCATTTTCTTCTTCCTTCTTGTCCCAAACCAAAGCCGCTCCCTTTCACGGCGGCCGGAGGAGAAAGAACTCGAAGCTCCAAATCGTCTCCGCCGTCTTCGAGCGGTTCACGGAGCGCGCGATCAAAGCCGTCATCTTTTCCCAGAGAGAAGCCAAAGCTCTGGGCCGGGACATGGTGTTTACTCAGCACCTCTTGCTCGGTTTGATCGCCGAGGAGGAGCAGCAGCATCGCCATCTCCATCCCAATTCGCGCGGGTTTCTCGGGTCGGGCATGACCCTGGAGCAGGCCCGCCGCGCCGTCCGGAGCATTTGGCGGCATAATAGTCAAAGTCAAAGTAAAAGTCAAGGTCAAGCTGCTTCTGCTGCTGGTTCTGGTTCGGGCTCTGCTAGGGATCTCTCGTTTTCGATTAGCGCAAAGAGAGTTCTGGAGGCTGCGCTGGAGTATTCGAGGAGTAGAGCTCATAATTTTATTGCACCGGAGCACATTGTCATCGGTTTATTGACCGTTGATGATGGGAGTGCTGGTCAGGTCCTTAAAAGACTGGGGGTAAATGTAAACCAGTTGTTAGCCGAGGCAGCCTCCAGACTTCAAATAGAGCTTGCCAGAGATGGTAGAGAACCATCTGGGGGATCTCAAAAGACGATTTCTAAAAAGTCAACTGCATCGTCTGAGAAAATCAAAGAAAAAAGTGCTTTGGAGCGGTTCTGTGTGGATCTCACTGCTCGTGCTAGTGAGGGACGCATTGATCCTGTTATTGGCCGGGATACTGAAGTACAGAGAATTATTCAGATACTTTGTCGCAGAACTAAAAATAATCCCATTCTTCTCGGTCAAAGTGGGGTTGGAAAGACAGCCATTGTTGAAGGTCTGGCAACTAGTATTGCACAGGCAGATGTCCCTGTTTATCTGTTGACAAAACGCGTAATGTCTTTGGACGTGGCACTGTTAATGGCTGGTGCAAAGGAGAGGGGAGAGTTGGAGGCGCGTGTTACCTCCTTAATAAGTGATGTACAAAAATCAGGAAATGTTATTCTGTTTATTGATGAAGTTCATACTCTTATTGAGTCCGGCACAGTTGGACGAGGAAATAAGGGGTCTGGTCTTGACATTGCTAATATAATGAAACCGGCACTTGGGAGGGGTAAATTACAGTGTATTGCATCAACTACTACCGATGAATACAGGATGCATCTTGAAAAGGATAAAGCGTTTGGACGAAGATTCCAACCTGTCTGGATCAATGAACCAAGCCAGGACGATGCAGTTAGGATTCTGTTTGGTTTGCGTGAGAAATATGAGGCCCATCACAACTGCATATATGCACCAGAAGCCATTAGTGCTGCTGTGTACCTGTCAGCAAGATATATTCCTGATAGATATCTTCCTGATAAAGCTATCGATCTCCTTGATGAGGCGGGAAGTAGAGCTCGTATGGAAGCCTTTAAGAAGAAAAAAGAAGAGCGGGTTGGTATACTTTCAAAGTCACCTGATGATTATTGGCAAGAAATTCGTACAGTTCAGGCAATGCATGAAGTGGTCCTATCGAGTGAACTGAAAAATGGAGCTGCTTCTGTGGATGATACCAGAGAACATATTCTAGAGTCCATTTCATCTTCCACAGTAGATGATGAACCTACTGTGGTGGGACCAAATGATATAGCAGCAGTTGCCTCACTATGGTCAGGAGTCCCTCTTCAGCAGCTCACTGCTGATGACCGACTGCTTTTGGTGGATCTTGATGAGAAGCTCAGAAGACGGGTTGTTGGTCAAGATGAGGCTGTTGCAGCCATTTCTCGAGCTGTTAGGAGGTCCCGGGTTGGATTGAAGGATCCAAGTAGACCCATGGCAACAATGCTCTTTTGCGGTCCTACTGGAGTTGGCAAAACTGAACTGACAAAAGCTTTGTCTGCATGTTATTTTGGATCGGAGGAAGCCATGGTACGACTGGACATGAGTGAATATATGGAGCGACACTCTGTCAGCAAATTAATAGGGTCACCTCCAGGCTATGTTGGCTTTGGAGAGGGAGGCACTCTAACAGAAGCTATCAGAAGACGACCTTTTACAGTAGTTGTGCTTGATGAAATCGAGAAGGCCCATCCAGATATATTCAACATCCTTCTCCAAATCTTTGAAGATGGCCACCTTACTGATTCTCAGGGACGAAGAGTATCGTTTAAGAATGCTTTGGTAGTGATGACATCTAATGTGGGTTCCACTATCATTGCAAAGGGTCGAAAAAGCTCCATTGGTTTTGTGCTTACAGACGATGAGTCAAGTACATATGCCGGGATAAAAGCAACAGTAATGGAAGAACTCAAGTCATATTTTCGTCCAGAGTTGCTGAACAGGATAGACGAAGTAGTTGTATTCCATCCCCTTGAAAAGTCTCAGATGCTGGAGATTGTAAATATAATGCTGCAAGAAGTGAAGCAGAGGCTAATATCTCTGGGAATTGGACTAGAGGTGTCTGAATCAGTCAAGAACCTCATATGCAAAGAAGGCTATGATCGGATCTATGGTGCTCGCCCTCTTAGACGAGCAATTACATTAATAATTGAAGACCCATTGAGTGAAGCCCTCCTAGCCGGAGTTTACCAGCCTGGTGACACTGCCGTCATTGATTTAGATGCTTCTGGGAACCCTTCTGTAAGTAATGGATCAGGTCAGAGTAGTATCCCTTTCTCTAAAACCAATATACAGCATCCATCTTGTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009941 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022607 GO:0031967 GO:0031975 GO:0033554 GO:0034214 GO:0034605 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046983 GO:0047484 GO:0048518 GO:0048583 GO:0048584 GO:0050789 GO:0050896 GO:0051259 GO:0051716 GO:0065003 GO:0065007 GO:0071840 GO:0080134 GO:1901000 GO:1901002 GO:1902584 GO:2000070

KEGG Pathways

Metabolic & Signaling

ko01100 map01100

Pfam Domains

Protein Families

AAA AAA_2 ClpB_D2-small Clp_N

Protein Analysis

967

Amino Acids

106.15

Weight (kDa)

8.9

Isoelectric Point (pI)

51.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	195 - 260	2.9e-11	Clp repeat (R) N-terminal domain
AAA_16	PF13191	312 - 365	8.4e-06	AAA ATPase domain
NBD_SMAX1	PF23569	318 - 419	5.8e-06	SMAX1 nucleotide binding domain
AAA	PF00004	335 - 451	6e-09	ATPase family associated with various cellular activities (AAA)
AAA_lid_9	PF17871	478 - 554	1.7e-26	AAA lid domain
AAA_2	PF07724	674 - 849	3e-63	AAA domain (Cdc48 subfamily)
AAA_5	PF07728	679 - 799	7.9e-11	AAA domain (dynein-related subfamily)
ClpB_D2-small	PF10431	855 - 934	2.9e-23	C-terminal, D2-small domain, of ClpB protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013822)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51070
fragaria_vesca	FvH4_5g10020
malus_domestica	MD06G1128400.v1.1
prunus_persica	Prupe.5G138200_v2.0.a1
pyrus_communis	pycom06g11860
rosa_chinensis	RchiOBHm_Chr7g0188241
rosa_laevigata	RLG00000004715
rosa_multiflora	Rmu_co8357845.1_g000001 Rmu_co8388145.1_g000001 Rmu_sc0008750.1_g000013
rosa_roxburghii	Rroxscaffold_3G00266580
rosa_rugosa	Rorug06G0489400
rosa_samantha	Rh7AG093700 Rh7BG095100 Rh7CG095500 Rh7DG095700
rosa_wichuraiana	Rw7G008070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2798
AccBSI	CCGCTC	4 cut(s)	221, 286, 883, 1645
AccI	GTMKAC	2 cut(s)	1654, 2020
AccII	CGCG	8 cut(s)	69, 300, 302, 433, 435, 477, 1104, 1168
AccIII	TCCGGA	1 cut(s)	483
AcoI	YGGCCR	3 cut(s)	235, 940, 2323
AcsI	RAATTY	1 cut(s)	1689
AcuI	CTGAAG	2 cut(s)	972, 1862
AfaI	GTAC	6 cut(s)	957, 1194, 1532, 1696, 2124, 2464
AfiI	CCNNNNNNNGG	8 cut(s)	230, 355, 466, 809, 938, 1316, 1594, 1860
AflIII	ACRYGT	2 cut(s)	67, 1102
AgsI	TTSAA	9 cut(s)	773, 1046, 1379, 1662, 2008, 2296, 2318, 2567, 2744
AhdI	GACNNNNNGTC	2 cut(s)	2159, 2455
AhlI	ACTAGT	1 cut(s)	1058
AjiI	CACGTC	1 cut(s)	1120
AjnI	CCWGG	4 cut(s)	459, 1434, 2185, 2785
AjuI	GAANNNNNNNTTGG	2 cut(s)	1632, 1664
Alw21I	GWGCWC	4 cut(s)	643, 666, 1612, 2710
Alw26I	GTCTC	2 cut(s)	274, 2577
AlwNI	CAGNNNCTG	1 cut(s)	2581
Ama87I	CYCGRG	3 cut(s)	443, 1977, 1995
Aor13HI	TCCGGA	1 cut(s)	483
AoxI	GGCC	7 cut(s)	235, 346, 468, 940, 1482, 2277, 2323
ApoI	RAATTY	1 cut(s)	1689
ArsI	GACNNNNNNTTYG	6 cut(s)	508, 540, 2068, 2072, 2100, 2104
AseI	ATTAAT	2 cut(s)	2171, 2735
Asp700I	GAANNNNTTC	1 cut(s)	1776
AspLEI	GCGC	7 cut(s)	300, 302, 435, 479, 596, 619, 1168
AspS9I	GGNCC	9 cut(s)	346, 469, 712, 1483, 1720, 1829, 1992, 2048, 2278
AsuC2I	CCSGG	5 cut(s)	350, 944, 1996, 1997, 2473
AsuHPI	GGTGA	3 cut(s)	1659, 2172, 2801
AvaI	CYCGRG	3 cut(s)	443, 1977, 1995
AvaII	GGWCC	5 cut(s)	712, 1720, 1829, 1992, 2048
BaeI	ACNNNNGTAYC	2 cut(s)	939, 972
BalI	TGGCCA	1 cut(s)	2325
BamHI	GGATCC	1 cut(s)	2011
BanII	GRGCYC	3 cut(s)	566, 643, 1612
BarI	GAAGNNNNNNTAC	2 cut(s)	2106, 2138
BauI	CACGAG	1 cut(s)	906
BbsI	GAAGAC	4 cut(s)	271, 1942, 2242, 2751
Bbv12I	GWGCWC	4 cut(s)	643, 666, 1612, 2710
BceAI	ACGGC	6 cut(s)	131, 247, 260, 296, 464, 2783
BcgI	CGANNNNNNTGC	4 cut(s)	392, 426, 1673, 1707
BciT130I	CCWGG	4 cut(s)	461, 1436, 2187, 2787
BciVI	GTATCC	2 cut(s)	940, 2870
BcnI	CCSGG	5 cut(s)	350, 944, 1996, 1997, 2473
BcoDI	GTCTC	2 cut(s)	274, 2577
BcuI	ACTAGT	1 cut(s)	1058
BfaI	CTAG	6 cut(s)	570, 912, 1059, 1781, 2645, 2768
BfuI	GTATCC	2 cut(s)	940, 2870
Bme1390I	CCNGG	9 cut(s)	350, 461, 944, 1436, 1996, 1997, 2187, 2473, 2787
Bme18I	GGWCC	5 cut(s)	712, 1720, 1829, 1992, 2048
BmeRI	GACNNNNNGTC	2 cut(s)	2159, 2455
BmeT110I	CYCGRG	3 cut(s)	443, 1977, 1995
BmgBI	CACGTC	1 cut(s)	1120
BmgT120I	GGNCC	9 cut(s)	346, 469, 712, 1483, 1720, 1829, 1992, 2048, 2278
BmiI	GGNNCC	5 cut(s)	1830, 1994, 2013, 2398, 2825
BmrFI	CCNGG	9 cut(s)	350, 461, 944, 1436, 1996, 1997, 2187, 2473, 2787
BmrI	ACTGGG	1 cut(s)	734
BmsI	GCATC	9 cut(s)	418, 854, 1352, 1359, 1381, 1432, 2568, 2803, 2896
BmuI	ACTGGG	1 cut(s)	734
BpiI	GAAGAC	4 cut(s)	271, 1942, 2242, 2751
BplI	GAGNNNNNCTC	6 cut(s)	625, 657, 746, 778, 2141, 2173
BpmI	CTGGAG	7 cut(s)	482, 629, 641, 748, 2076, 2169, 2603
BpuEI	CTTGAG	1 cut(s)	2486
BpuMI	CCSGG	5 cut(s)	350, 944, 1996, 1997, 2473
Bsa29I	ATCGAT	1 cut(s)	1581
BsaBI	GATNNNNATC	1 cut(s)	1560
BsaJI	CCNNGG	6 cut(s)	393, 459, 753, 1995, 2025, 2118
BsaWI	WCCGGW	2 cut(s)	483, 658
BsaXI	ACNNNNNCTCC	2 cut(s)	691, 721
Bsc4I	CCNNNNNNNGG	8 cut(s)	230, 355, 466, 809, 938, 1316, 1594, 1860
Bse118I	RCCGGY	1 cut(s)	1309
Bse1I	ACTGG	4 cut(s)	729, 742, 2059, 2133
Bse3DI	GCAATG	3 cut(s)	1290, 1713, 2406
Bse8I	GATNNNNATC	1 cut(s)	1560
BseAI	TCCGGA	1 cut(s)	483
BseBI	CCWGG	4 cut(s)	461, 1436, 2187, 2787
BseCI	ATCGAT	1 cut(s)	1581
BseDI	CCNNGG	6 cut(s)	393, 459, 753, 1995, 2025, 2118
BseGI	GGATG	8 cut(s)	14, 421, 1374, 1765, 2281, 2298, 2557, 2887
BseJI	GATNNNNATC	1 cut(s)	1560
BseLI	CCNNNNNNNGG	8 cut(s)	230, 355, 466, 809, 938, 1316, 1594, 1860
BseMI	GCAATG	3 cut(s)	1290, 1713, 2406
BseMII	CTCAG	5 cut(s)	380, 843, 1945, 2354, 2588
BseNI	ACTGG	4 cut(s)	729, 742, 2059, 2133
BsePI	GCGCGC	1 cut(s)	298
BseRI	GAGGAG	5 cut(s)	10, 256, 410, 413, 646
BseX3I	CGGCCG	1 cut(s)	235
Bsh1236I	CGCG	8 cut(s)	69, 300, 302, 433, 435, 477, 1104, 1168
Bsh1285I	CGRYCG	1 cut(s)	238
BshFI	GGCC	7 cut(s)	237, 348, 470, 942, 1484, 2279, 2325
BshVI	ATCGAT	1 cut(s)	1581
BsiEI	CGRYCG	1 cut(s)	238
BsiHKAI	GWGCWC	4 cut(s)	643, 666, 1612, 2710
BsiHKCI	CYCGRG	3 cut(s)	443, 1977, 1995
BslFI	GGGAC	8 cut(s)	190, 365, 933, 1064, 1842, 1856, 1978, 2358
BslI	CCNNNNNNNGG	8 cut(s)	230, 355, 466, 809, 938, 1316, 1594, 1860
BsmAI	GTCTC	2 cut(s)	274, 2577
BsmBI	CGTCTC	1 cut(s)	274
BsmFI	GGGAC	8 cut(s)	190, 365, 933, 1064, 1842, 1856, 1978, 2358
BsmI	GAATGC	1 cut(s)	2371
BsnI	GGCC	7 cut(s)	237, 348, 470, 942, 1484, 2279, 2325
BsoBI	CYCGRG	3 cut(s)	443, 1977, 1995
Bsp1286I	GDGCHC	5 cut(s)	566, 643, 666, 1612, 2710
Bsp13I	TCCGGA	1 cut(s)	483
Bsp1407I	TGTACA	1 cut(s)	1192
Bsp19I	CCATGG	2 cut(s)	2025, 2118
BspANI	GGCC	7 cut(s)	237, 348, 470, 942, 1484, 2279, 2325
BspCNI	CTCAG	5 cut(s)	379, 844, 1944, 2353, 2587
BspDI	ATCGAT	1 cut(s)	1581
BspEI	TCCGGA	1 cut(s)	483
BspFNI	CGCG	8 cut(s)	69, 300, 302, 433, 435, 477, 1104, 1168
BspLI	GGNNCC	5 cut(s)	1830, 1994, 2013, 2398, 2825
BspQI	GCTCTTC	1 cut(s)	1635
BsrBI	CCGCTC	4 cut(s)	221, 286, 883, 1645
BsrDI	GCAATG	3 cut(s)	1290, 1713, 2406
BsrFI	RCCGGY	1 cut(s)	1309
BsrGI	TGTACA	1 cut(s)	1192
BsrI	ACTGG	4 cut(s)	729, 742, 2059, 2133
BssAI	RCCGGY	1 cut(s)	1309
BssECI	CCNNGG	6 cut(s)	393, 459, 753, 1995, 2025, 2118
BssHII	GCGCGC	1 cut(s)	298
BssNAI	GTATAC	1 cut(s)	1655
BssSI	CACGAG	1 cut(s)	906
BssT1I	CCWWGG	2 cut(s)	2025, 2118
Bst1107I	GTATAC	1 cut(s)	1655
Bst2BI	CACGAG	1 cut(s)	906
Bst2UI	CCWGG	4 cut(s)	461, 1436, 2187, 2787
Bst4CI	ACNGT	9 cut(s)	688, 1128, 1258, 1335, 1699, 1804, 1822, 2251, 2488
Bst6I	CTCTTC	3 cut(s)	1635, 1881, 2344
BstAPI	GCANNNNNTGC	2 cut(s)	376, 1850
BstAUI	TGTACA	1 cut(s)	1192
BstC8I	GCNNGC	5 cut(s)	300, 468, 472, 783, 2710
BstDEI	CTNAG	6 cut(s)	366, 852, 1931, 2340, 2574, 2717
BstDSI	CCRYGG	2 cut(s)	2025, 2118
BstEII	GGTNACC	1 cut(s)	2178
BstENI	CCTNNNNNAGG	2 cut(s)	464, 1592
BstF5I	GGATG	8 cut(s)	14, 421, 1374, 1765, 2281, 2298, 2557, 2887
BstFNI	CGCG	8 cut(s)	69, 300, 302, 433, 435, 477, 1104, 1168
BstHHI	GCGC	7 cut(s)	300, 302, 435, 479, 596, 619, 1168
BstMAI	GTCTC	2 cut(s)	274, 2577
BstMCI	CGRYCG	1 cut(s)	238
BstNI	CCWGG	4 cut(s)	461, 1436, 2187, 2787
BstNSI	RCATGY	2 cut(s)	8, 2097
BstPI	GGTNACC	1 cut(s)	2178
BstSCI	CCNGG	9 cut(s)	348, 459, 942, 1434, 1994, 1995, 2185, 2471, 2785
BstUI	CGCG	8 cut(s)	69, 300, 302, 433, 435, 477, 1104, 1168
BstV2I	GAAGAC	4 cut(s)	271, 1942, 2242, 2751
BstX2I	RGATCY	6 cut(s)	574, 812, 895, 1915, 2011, 2697
BstXI	CCANNNNNNTGG	2 cut(s)	48, 343
BstYI	RGATCY	6 cut(s)	574, 812, 895, 1915, 2011, 2697
BstZ17I	GTATAC	1 cut(s)	1655
BstZI	CGGCCG	1 cut(s)	235
Bsu15I	ATCGAT	1 cut(s)	1581
BsuI	GTATCC	2 cut(s)	940, 2870
BsuRI	GGCC	7 cut(s)	237, 348, 470, 942, 1484, 2279, 2325
BsuTUI	ATCGAT	1 cut(s)	1581
BtgI	CCRYGG	2 cut(s)	2025, 2118
BtgZI	GCGATG	1 cut(s)	395
BtrI	CACGTC	1 cut(s)	1120
BtsCI	GGATG	8 cut(s)	14, 421, 1374, 1765, 2281, 2298, 2557, 2887
BtsI	GCAGTG	3 cut(s)	900, 1887, 2793
BtsIMutI	CAGTG	5 cut(s)	900, 1124, 1340, 1887, 2793
Cac8I	GCNNGC	5 cut(s)	300, 468, 472, 783, 2710
CaiI	CAGNNNCTG	1 cut(s)	2581
CfoI	GCGC	7 cut(s)	300, 302, 435, 479, 596, 619, 1168
Cfr10I	RCCGGY	1 cut(s)	1309
Cfr13I	GGNCC	9 cut(s)	346, 469, 712, 1483, 1720, 1829, 1992, 2048, 2278
Cfr9I	CCCGGG	1 cut(s)	1995
ClaI	ATCGAT	1 cut(s)	1581
CseI	GACGC	1 cut(s)	930
Csp6I	GTAC	6 cut(s)	956, 1193, 1531, 1695, 2123, 2463
CspCI	CAANNNNNGTGG	2 cut(s)	2389, 2424
CviAII	CATG	8 cut(s)	5, 355, 454, 1712, 2026, 2094, 2119, 2134
CviQI	GTAC	6 cut(s)	956, 1193, 1531, 1695, 2123, 2463
DdeI	CTNAG	6 cut(s)	366, 852, 1931, 2340, 2574, 2717
DrdI	GACNNNNNNGTC	1 cut(s)	2798
DriI	GACNNNNNGTC	2 cut(s)	2159, 2455
DseDI	GACNNNNNNGTC	1 cut(s)	2798
EaeI	YGGCCR	3 cut(s)	235, 940, 2323
EagI	CGGCCG	1 cut(s)	235
Eam1104I	CTCTTC	3 cut(s)	1635, 1881, 2344
Eam1105I	GACNNNNNGTC	2 cut(s)	2159, 2455
EarI	CTCTTC	3 cut(s)	1635, 1881, 2344
EciI	GGCGGA	1 cut(s)	262
Ecl136II	GAGCTC	2 cut(s)	641, 1610
EclXI	CGGCCG	1 cut(s)	235
Eco130I	CCWWGG	2 cut(s)	2025, 2118
Eco24I	GRGCYC	3 cut(s)	566, 643, 1612
Eco32I	GATATC	2 cut(s)	33, 1562
Eco47I	GGWCC	5 cut(s)	712, 1720, 1829, 1992, 2048
Eco52I	CGGCCG	1 cut(s)	235
Eco53kI	GAGCTC	2 cut(s)	641, 1610
Eco57I	CTGAAG	2 cut(s)	972, 1862
Eco88I	CYCGRG	3 cut(s)	443, 1977, 1995
Eco91I	GGTNACC	1 cut(s)	2178
EcoICRI	GAGCTC	2 cut(s)	641, 1610
EcoNI	CCTNNNNNAGG	2 cut(s)	464, 1592
EcoO109I	RGGNCCY	2 cut(s)	712, 1992
EcoO65I	GGTNACC	1 cut(s)	2178
EcoRII	CCWGG	4 cut(s)	459, 1434, 2185, 2785
EcoRV	GATATC	2 cut(s)	33, 1562
EcoT14I	CCWWGG	2 cut(s)	2025, 2118
EcoT22I	ATGCAT	3 cut(s)	6, 1374, 1713
EcoT38I	GRGCYC	3 cut(s)	566, 643, 1612
ErhI	CCWWGG	2 cut(s)	2025, 2118
Esp3I	CGTCTC	1 cut(s)	274
FaeI	CATG	8 cut(s)	8, 358, 457, 1715, 2029, 2097, 2122, 2137
FalI	AAGNNNNNCTT	2 cut(s)	2227, 2259
FaqI	GGGAC	8 cut(s)	190, 365, 933, 1064, 1842, 1856, 1978, 2358
FatI	CATG	8 cut(s)	4, 354, 453, 1711, 2025, 2093, 2118, 2133
FauI	CCCGC	4 cut(s)	428, 479, 1591, 1638
FauNDI	CATATG	2 cut(s)	2467, 2675
FblI	GTMKAC	2 cut(s)	1654, 2020
FokI	GGATG	7 cut(s)	408, 1381, 1772, 2268, 2285, 2544, 2874
FriOI	GRGCYC	3 cut(s)	566, 643, 1612
FspBI	CTAG	6 cut(s)	570, 912, 1059, 1781, 2645, 2768
GlaI	GCGC	7 cut(s)	299, 301, 434, 478, 595, 618, 1167
GsuI	CTGGAG	7 cut(s)	482, 629, 641, 748, 2076, 2169, 2603
HaeIII	GGCC	7 cut(s)	237, 348, 470, 942, 1484, 2279, 2325
HgaI	GACGC	1 cut(s)	930
HhaI	GCGC	7 cut(s)	300, 302, 435, 479, 596, 619, 1168
Hin1II	CATG	8 cut(s)	8, 358, 457, 1715, 2029, 2097, 2122, 2137
Hin6I	GCGC	7 cut(s)	298, 300, 433, 477, 594, 617, 1166
HinP1I	GCGC	7 cut(s)	298, 300, 433, 477, 594, 617, 1166
HincII	GTYRAC	2 cut(s)	840, 1095
HindII	GTYRAC	2 cut(s)	840, 1095
HindIII	AAGCTT	1 cut(s)	2082
HinfI	GANTC	8 cut(s)	1247, 1404, 1453, 1784, 1869, 2336, 2456, 2657
HphI	GGTGA	3 cut(s)	1659, 2172, 2801
Hpy188I	TCNGA	9 cut(s)	853, 972, 1934, 2110, 2234, 2577, 2656, 2697, 2853
Hpy99I	CGWCG	1 cut(s)	152
HpyAV	CCTTC	7 cut(s)	208, 1040, 2002, 2269, 2312, 2678, 2838
HpyCH4III	ACNGT	9 cut(s)	688, 1128, 1258, 1335, 1699, 1804, 1822, 2251, 2488
HpyCH4IV	ACGT	1 cut(s)	1119
HpyF3I	CTNAG	6 cut(s)	366, 852, 1931, 2340, 2574, 2717
HpySE526I	ACGT	1 cut(s)	1119
Hsp92II	CATG	8 cut(s)	8, 358, 457, 1715, 2029, 2097, 2122, 2137
HspAI	GCGC	7 cut(s)	298, 300, 433, 477, 594, 617, 1166
Kpn2I	TCCGGA	1 cut(s)	483
LguI	GCTCTTC	1 cut(s)	1635
LweI	GCATC	9 cut(s)	418, 854, 1352, 1359, 1381, 1432, 2568, 2803, 2896
MaeI	CTAG	6 cut(s)	570, 912, 1059, 1781, 2645, 2768
MaeII	ACGT	1 cut(s)	1119
MaeIII	GTNAC	6 cut(s)	100, 122, 1171, 1665, 2178, 2789
MbiI	CCGCTC	4 cut(s)	221, 286, 883, 1645
MflI	RGATCY	6 cut(s)	574, 812, 895, 1915, 2011, 2697
MhlI	GDGCHC	5 cut(s)	566, 643, 666, 1612, 2710
MlsI	TGGCCA	1 cut(s)	2325
MluI	ACGCGT	2 cut(s)	67, 1102
MluNI	TGGCCA	1 cut(s)	2325
MlyI	GAGTC	4 cut(s)	1256, 1793, 1878, 2465
MmeI	TCCRAC	5 cut(s)	1006, 1140, 1240, 1432, 1981
Mox20I	TGGCCA	1 cut(s)	2325
Mph1103I	ATGCAT	3 cut(s)	6, 1374, 1713
MroI	TCCGGA	1 cut(s)	483
MroXI	GAANNNNTTC	1 cut(s)	1776
MscI	TGGCCA	1 cut(s)	2325
MseI	TTAA	8 cut(s)	717, 1130, 1181, 1626, 2171, 2361, 2735, 2902
MslI	CAYNNNNRTG	1 cut(s)	46
Msp20I	TGGCCA	1 cut(s)	2325
MspR9I	CCNGG	9 cut(s)	350, 461, 944, 1436, 1996, 1997, 2187, 2473, 2787
Mva1269I	GAATGC	1 cut(s)	2371
MvaI	CCWGG	4 cut(s)	461, 1436, 2187, 2787
MvnI	CGCG	8 cut(s)	69, 300, 302, 433, 435, 477, 1104, 1168
NciI	CCSGG	5 cut(s)	350, 944, 1996, 1997, 2473
NcoI	CCATGG	2 cut(s)	2025, 2118
NdeI	CATATG	2 cut(s)	2467, 2675
NlaIII	CATG	8 cut(s)	8, 358, 457, 1715, 2029, 2097, 2122, 2137
NlaIV	GGNNCC	5 cut(s)	1830, 1994, 2013, 2398, 2825
NmeAIII	GCCGAG	2 cut(s)	418, 778
NmuCI	GTSAC	3 cut(s)	1665, 2178, 2789
NsiI	ATGCAT	3 cut(s)	6, 1374, 1713
NspI	RCATGY	2 cut(s)	8, 2097
PaeR7I	CTCGAG	1 cut(s)	1977
PauI	GCGCGC	1 cut(s)	298
PciSI	GCTCTTC	1 cut(s)	1635
PcsI	WCGNNNNNNNCGW	2 cut(s)	273, 299
PctI	GAATGC	1 cut(s)	2371
PdmI	GAANNNNTTC	1 cut(s)	1776
PfeI	GAWTC	4 cut(s)	1404, 1453, 2336, 2657
PleI	GAGTC	4 cut(s)	1255, 1792, 1877, 2464
PpsI	GAGTC	4 cut(s)	1255, 1792, 1877, 2464
PpuMI	RGGWCCY	2 cut(s)	712, 1992
PshBI	ATTAAT	2 cut(s)	2171, 2735
Psp124BI	GAGCTC	2 cut(s)	643, 1612
Psp5II	RGGWCCY	2 cut(s)	712, 1992
Psp6I	CCWGG	4 cut(s)	459, 1434, 2185, 2785
PspEI	GGTNACC	1 cut(s)	2178
PspGI	CCWGG	4 cut(s)	459, 1434, 2185, 2785
PspN4I	GGNNCC	5 cut(s)	1830, 1994, 2013, 2398, 2825
PspPI	GGNCC	9 cut(s)	346, 469, 712, 1483, 1720, 1829, 1992, 2048, 2278
PspPPI	RGGWCCY	2 cut(s)	712, 1992
PstNI	CAGNNNCTG	1 cut(s)	2581
PsuI	RGATCY	6 cut(s)	574, 812, 895, 1915, 2011, 2697
PteI	GCGCGC	1 cut(s)	298
RsaI	GTAC	6 cut(s)	957, 1194, 1532, 1696, 2124, 2464
RsaNI	GTAC	6 cut(s)	956, 1193, 1531, 1695, 2123, 2463
RseI	CAYNNNNRTG	1 cut(s)	46
SacI	GAGCTC	2 cut(s)	643, 1612
SapI	GCTCTTC	1 cut(s)	1635
SaqAI	TTAA	8 cut(s)	717, 1130, 1181, 1626, 2171, 2361, 2735, 2902
Sau96I	GGNCC	9 cut(s)	346, 469, 712, 1483, 1720, 1829, 1992, 2048, 2278
SchI	GAGTC	4 cut(s)	1256, 1793, 1878, 2465
ScrFI	CCNGG	9 cut(s)	350, 461, 944, 1436, 1996, 1997, 2187, 2473, 2787
SduI	GDGCHC	5 cut(s)	566, 643, 666, 1612, 2710
SfaNI	GCATC	9 cut(s)	418, 854, 1352, 1359, 1381, 1432, 2568, 2803, 2896
Sfr274I	CTCGAG	1 cut(s)	1977
SinI	GGWCC	5 cut(s)	712, 1720, 1829, 1992, 2048
SlaI	CTCGAG	1 cut(s)	1977
SmaI	CCCGGG	1 cut(s)	1997
SmiMI	CAYNNNNRTG	1 cut(s)	46
SmlI	CTYRAG	2 cut(s)	1977, 2501
SmoI	CTYRAG	2 cut(s)	1977, 2501
SpeI	ACTAGT	1 cut(s)	1058
SspMI	CTAG	6 cut(s)	570, 912, 1059, 1781, 2645, 2768
SstI	GAGCTC	2 cut(s)	643, 1612
StyD4I	CCNGG	9 cut(s)	348, 459, 942, 1434, 1994, 1995, 2185, 2471, 2785
StyI	CCWWGG	2 cut(s)	2025, 2118
TaaI	ACNGT	9 cut(s)	688, 1128, 1258, 1335, 1699, 1804, 1822, 2251, 2488
TaiI	ACGT	1 cut(s)	1122
TaqI	TCGA	9 cut(s)	254, 282, 587, 629, 1581, 1727, 1978, 2271, 2419
TaqII	GACCGA	2 cut(s)	1001, 1914
TatI	WGTACW	3 cut(s)	955, 1192, 2462
TauI	GCSGC	4 cut(s)	221, 237, 477, 499
TfiI	GAWTC	4 cut(s)	1404, 1453, 2336, 2657
Tru1I	TTAA	8 cut(s)	717, 1130, 1181, 1626, 2171, 2361, 2735, 2902
Tru9I	TTAA	8 cut(s)	717, 1130, 1181, 1626, 2171, 2361, 2735, 2902
TscAI	CASTG	5 cut(s)	907, 1131, 1340, 1894, 2800
TseFI	GTSAC	3 cut(s)	1665, 2178, 2789
Tsp45I	GTSAC	3 cut(s)	1665, 2178, 2789
TspDTI	ATGAA	9 cut(s)	1223, 1242, 1319, 1374, 1440, 1728, 1782, 1827, 2280
TspGWI	ACGGA	2 cut(s)	13, 308
TspMI	CCCGGG	1 cut(s)	1995
TspRI	CASTG	5 cut(s)	907, 1131, 1340, 1894, 2800
VpaK11BI	GGWCC	5 cut(s)	712, 1720, 1829, 1992, 2048
VspI	ATTAAT	2 cut(s)	2171, 2735
XagI	CCTNNNNNAGG	2 cut(s)	464, 1592
XapI	RAATTY	1 cut(s)	1689
XbaI	TCTAGA	1 cut(s)	1780
XceI	RCATGY	2 cut(s)	8, 2097
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2125
XhoI	CTCGAG	1 cut(s)	1977
XmaI	CCCGGG	1 cut(s)	1995
XmiI	GTMKAC	2 cut(s)	1654, 2020
XmnI	GAANNNNTTC	1 cut(s)	1776
XspI	CTAG	6 cut(s)	570, 912, 1059, 1781, 2645, 2768
Zsp2I	ATGCAT	3 cut(s)	6, 1374, 1713

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.