Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7C

Orthogroup ID

OG0013822

Physical Location & Seq

Genomic Coordinates Forward (+)

7227108 .. 7233887

Gene Structure 6780 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7CG095500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2880 bp

ATGCATGTGTCGTCATCCTCCTCCGTGCTGATATCATGTCCACTCCAATGGAGAACTCCATCTCAACACGTATCATTCACTCATCTTCCCTTACAATCCGTTACGACTTGCACTACTATCAGATACGCCCGTGATCGCAGAAACGCCGTCGTTTCCCTCTACCCAATTCGCCTTATACACAACCCATTTTCTTCTTCCTTCTTGTCCCAAACCAAAGCCGCTCCCTTTCACGGCGGCCGGAGGAGAAAGAACTCGAAGCTCCAAATCGTCTCCGCCGTCTTCGAGCGGTTCACGGAGCGCGCGATCAAAGCCGTCATCTTTTCCCAGAGAGAAGCCAAAGCTCTGGGCCGGGACATGGTGTTTACTCAGCACCTCTTGCTCGGTTTGATCGCCGAGGAGGAGCAGCAGCATCGCCATCTTCATCCCAATTCGCGCGGGTTTCTCGGGTCGGGCATGACCCTGGAGCAGGCCCGCCGCGCCGTCCGGAGCATTTGGCGGCATAATAGTCAAAGTCAAAGTCAAGGTCAAGCTGCTGCTTCGGGTTCTGCTAGGGATCTCTCGTTTTCGATTAGCACAAAGAGAGTTCTGGAGGCTGCGCTGGAGTATTCGAGGAGTAGAGCTCATAATTTTATTGCACCGGAGCACATTGTCATCGGTTTATTGACTGTTGATGATGGGAGTGCTGGTCAGGTCCTTAAAAGACTGGGGGTAAATGTAAACCAGTTGTTAGCTGAGGCAGCCTCCAGACTTCAAATAGAGCTTGCCAAAGATGGTAGAGAGCCATCTGGGGGATCTCAAAAGACATTTTCTAAAAAGTCAACTGCATCGTCTGAGAAAACCAAAGAAAAAAGTGCTTTGGAGCGGTTCTGTGTGGATCTCACTGCTCGTGCTAGTGAGGGACGCATTGATCCTGTTATTGGCCGGGATACTGAAGTTCAGAGAATTATTCAGATACTTTGTCGCAGAACTAAAAATAATCCCATTCTTCTCGGTCAAAGTGGGGTTGGAAAGACAGCCATTGTTGAAGGTCTGGCAACTAGTATTGCACAGGCAGATGTCCCTGTTTATCTGTTGACAAAACGCGTAATGTCTTTGGACGTGGCACTGTTAATGGCTGGTGCAAAGGAGAGGGGAGAGTTGGAGGCGCGTGTTACCTCCTTAATAAGTGATGTACAAAAATCAGGAAATGTTATTTTGTTTATTGATGAAGTTCATACTCTTATTGAGTCCGGCACAGTTGGACGAGGAAATAAGGGGTCTGGTCTTGACATTGCTAATATAATGAAACCGGCACTTGGGAGGGGTAAATTACAGTGTATTGCATCAACTACTACCGATGAATACAGGATGCATCTTGAAAAGGATAAAGCATTTGGACGAAGATTCCAACCTGTCTGGATCAATGAACCAAGCCAGGACGATGCAGTTAGGATTCTGTTTGGTTTGCGTGAGAAATATGAGGCCCATCACAACTGCATATATTCACCAGAAGCCATTAGTGCTGCTGTGTACCTGTCAGCAAGATATATTCCTGATAGATATCTTCCTGATAAAGCTATCGATCTCCTTGATGAGGCGGGAAGTAGAGCTCGTATGGAAGCCTTTAAGAAGAAAAAAGTAGAGCGGGTTGGCATACTTTCAAAGTCACCTGATGATTATTGGCAAGAAATTCGTACAGTTCAGGCAATGCATGAAGTGGTCCTATCGAGTGAACTGAAAAATGGAGCTGCTTCTGTGGATGATACCAGAGAACATATTCTAGAGTCCAGTTCATCTTCCACAGTAGATGATGAACCTACTGTGGTGGGACCAAATGATATAGCAGCAGTTGCCTCACTATGGTCAGGAGTCCCTCTTCAGCAGCTCACTGCTGATGACCGACTGCTTTTGGTGGGTCTTGATGAGAAGCTCAGAAGACGGGTTGTTGGTCAAGATGAGGCTGTTGCAGCCATTTCTCGAGCTGTTAGGAGGTCCCGGGTTGGATTGAAGGATCCAAGTAGACCCATGGCAACAATGCTCTTTTGCGGTCCTACTGGAGTTGGCAAAACTGAACTGACAAAAGCTTTGTCTGCATGTTATTTTGGATCGGAGGAAGCTATGGTACGACTGGACATGAGTGAATATATGGAGCGGCACTCTGTCAGCAAATTAATAGGGTCACCTCCAGGCTATGTTGGCTTTGGAGAGGGAGGCACTCTAACAGAAGCTATCAGAAGACGACCTTTTACAGTAGTTGTGCTTGATGAAATCGAGAAGGCCCATCCAGATATATTCAACATCCTTCTCCAAATCTTTGAAGATGGCCACCTTACTGATTCTCAGGGACGAAGAGTATCATTTAAGAATGCTTTGGTAGTGATGACATCTAATGTGGGTTCCACTATCATTGCAAAGGGTCGAAAAAGCTCCATTGGTTTTGTGCTTACAGACGACGAGTCAAGTACATATGCCGGGATAAAAGCAACAGTAATGGAAGAACTCAAGTCATATTTTCGTCCAGAGTTGCTGAACAGGATAGACGAAGTAGTTGTATTCCATCCCCTTGAAAAGTCTCAGATGCTGGAGATTGTAAATATAATGCTGCAAGAAGTGAAGCAGAGGCTAATATCTCTGGGAATTGGACTAGAGGTGTCTGAATCAGTCAAGAACCTCATATGCAAAGAAGGCTATGATCGGATCTATGGCGCTCGCCCTCTTAGACGAGCAATTACATTAATAATTGAAGACCCATTGAGTGAAGCCCTCCTTGCTGGAGTCTACCAGCCTGGTGACACTGCCGTCATTGATTTAGATGCTTCTGGGATCCCTTCTGTAAGTAATGGATCAGCAGCCAGTGCCAGAGAGTACTTGGGGCTTGGTTTGGTCAAGGGGAAGAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009941 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022607 GO:0031967 GO:0031975 GO:0033554 GO:0034214 GO:0034605 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046983 GO:0047484 GO:0048518 GO:0048583 GO:0048584 GO:0050789 GO:0050896 GO:0051259 GO:0051716 GO:0065003 GO:0065007 GO:0071840 GO:0080134 GO:1901000 GO:1901002 GO:1902584 GO:2000070

KEGG Pathways

Metabolic & Signaling

ko01100 map01100

Pfam Domains

Protein Families

AAA AAA_2 ClpB_D2-small Clp_N

Protein Analysis

959

Amino Acids

105.37

Weight (kDa)

9.01

Isoelectric Point (pI)

50.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	188 - 258	2.9e-11	Clp repeat (R) N-terminal domain
AAA_16	PF13191	305 - 358	8.3e-06	AAA ATPase domain
NBD_SMAX1	PF23569	311 - 412	5.8e-06	SMAX1 nucleotide binding domain
AAA	PF00004	328 - 444	5.9e-09	ATPase family associated with various cellular activities (AAA)
AAA_lid_9	PF17871	471 - 540	1.3e-26	AAA lid domain
AAA_2	PF07724	667 - 842	3e-63	AAA domain (Cdc48 subfamily)
AAA_5	PF07728	672 - 792	7.8e-11	AAA domain (dynein-related subfamily)
ClpB_D2-small	PF10431	848 - 927	2.9e-23	C-terminal, D2-small domain, of ClpB protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013822)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51070
fragaria_vesca	FvH4_5g10020
malus_domestica	MD06G1128400.v1.1
prunus_persica	Prupe.5G138200_v2.0.a1
pyrus_communis	pycom06g11860
rosa_chinensis	RchiOBHm_Chr7g0188241
rosa_laevigata	RLG00000004715
rosa_multiflora	Rmu_co8357845.1_g000001 Rmu_co8388145.1_g000001 Rmu_sc0008750.1_g000013
rosa_roxburghii	Rroxscaffold_3G00266580
rosa_rugosa	Rorug06G0489400
rosa_samantha	Rh7AG093700 Rh7BG095100 Rh7CG095500 Rh7DG095700
rosa_wichuraiana	Rw7G008070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2777
AccBSI	CCGCTC	5 cut(s)	221, 286, 862, 1624, 2131
AccI	GTMKAC	2 cut(s)	1999, 2757
AccII	CGCG	7 cut(s)	300, 302, 433, 435, 477, 1083, 1147
AccIII	TCCGGA	1 cut(s)	483
AcoI	YGGCCR	3 cut(s)	235, 919, 2302
AcsI	RAATTY	1 cut(s)	1668
AcuI	CTGAAG	2 cut(s)	951, 1841
AfaI	GTAC	6 cut(s)	1173, 1511, 1675, 2103, 2443, 2846
AfiI	CCNNNNNNNGG	8 cut(s)	230, 355, 466, 788, 917, 1295, 1573, 1839
AflIII	ACRYGT	2 cut(s)	67, 1081
AgsI	TTSAA	9 cut(s)	752, 1025, 1358, 1641, 1987, 2275, 2297, 2546, 2723
AhdI	GACNNNNNGTC	1 cut(s)	2434
AhlI	ACTAGT	1 cut(s)	1037
AjiI	CACGTC	1 cut(s)	1099
AjnI	CCWGG	4 cut(s)	459, 1413, 2164, 2764
Alw21I	GWGCWC	3 cut(s)	622, 645, 1591
Alw26I	GTCTC	2 cut(s)	274, 2556
AlwNI	CAGNNNCTG	1 cut(s)	2560
Ama87I	CYCGRG	3 cut(s)	443, 1956, 1974
Aor13HI	TCCGGA	1 cut(s)	483
AoxI	GGCC	7 cut(s)	235, 346, 468, 919, 1461, 2256, 2302
ApoI	RAATTY	1 cut(s)	1668
ArsI	GACNNNNNNTTYG	8 cut(s)	502, 508, 534, 540, 2047, 2051, 2079, 2083
AseI	ATTAAT	2 cut(s)	2150, 2714
Asp700I	GAANNNNTTC	1 cut(s)	1755
AspLEI	GCGC	7 cut(s)	300, 302, 435, 479, 598, 1147, 2687
AspS9I	GGNCC	9 cut(s)	346, 469, 691, 1462, 1699, 1808, 1971, 2027, 2257
AsuC2I	CCSGG	5 cut(s)	350, 923, 1975, 1976, 2452
AsuHPI	GGTGA	4 cut(s)	1476, 1638, 2151, 2780
AvaI	CYCGRG	3 cut(s)	443, 1956, 1974
AvaII	GGWCC	5 cut(s)	691, 1699, 1808, 1971, 2027
BaeI	ACNNNNGTAYC	4 cut(s)	115, 148, 918, 951
BalI	TGGCCA	1 cut(s)	2304
BamHI	GGATCC	2 cut(s)	1990, 2802
BanII	GRGCYC	2 cut(s)	622, 1591
BarI	GAAGNNNNNNTAC	2 cut(s)	2085, 2117
BauI	CACGAG	1 cut(s)	885
BbsI	GAAGAC	4 cut(s)	271, 1921, 2221, 2730
Bbv12I	GWGCWC	3 cut(s)	622, 645, 1591
BbvCI	CCTCAGC	1 cut(s)	732
BccI	CCATC	9 cut(s)	67, 423, 668, 764, 790, 1473, 2268, 2294, 2544
BceAI	ACGGC	6 cut(s)	131, 247, 260, 296, 464, 2762
BcgI	CGANNNNNNTGC	4 cut(s)	392, 426, 1652, 1686
BciT130I	CCWGG	4 cut(s)	461, 1415, 2166, 2766
BciVI	GTATCC	1 cut(s)	919
BcnI	CCSGG	5 cut(s)	350, 923, 1975, 1976, 2452
BcoDI	GTCTC	2 cut(s)	274, 2556
BcuI	ACTAGT	1 cut(s)	1037
BfaI	CTAG	5 cut(s)	549, 891, 1038, 1760, 2624
BfoI	RGCGCY	1 cut(s)	2688
BfuI	GTATCC	1 cut(s)	919
BmcAI	AGTACT	1 cut(s)	2846
Bme1390I	CCNGG	9 cut(s)	350, 461, 923, 1415, 1975, 1976, 2166, 2452, 2766
Bme18I	GGWCC	5 cut(s)	691, 1699, 1808, 1971, 2027
BmeRI	GACNNNNNGTC	1 cut(s)	2434
BmeT110I	CYCGRG	3 cut(s)	443, 1956, 1974
BmgBI	CACGTC	1 cut(s)	1099
BmgT120I	GGNCC	9 cut(s)	346, 469, 691, 1462, 1699, 1808, 1971, 2027, 2257
BmiI	GGNNCC	5 cut(s)	1809, 1973, 1992, 2377, 2804
BmrFI	CCNGG	9 cut(s)	350, 461, 923, 1415, 1975, 1976, 2166, 2452, 2766
BmrI	ACTGGG	1 cut(s)	713
BmsI	GCATC	8 cut(s)	418, 833, 1331, 1338, 1360, 1411, 2547, 2782
BmuI	ACTGGG	1 cut(s)	713
BpiI	GAAGAC	4 cut(s)	271, 1921, 2221, 2730
BplI	GAGNNNNNCTC	6 cut(s)	604, 636, 725, 757, 2120, 2152
BpmI	CTGGAG	8 cut(s)	482, 608, 620, 727, 2055, 2148, 2582, 2772
Bpu10I	CCTNAGC	1 cut(s)	732
BpuEI	CTTGAG	1 cut(s)	2465
BpuMI	CCSGG	5 cut(s)	350, 923, 1975, 1976, 2452
Bsa29I	ATCGAT	1 cut(s)	1560
BsaAI	YACGTR	1 cut(s)	70
BsaBI	GATNNNNATC	1 cut(s)	1539
BsaJI	CCNNGG	4 cut(s)	393, 459, 1974, 2004
BsaWI	WCCGGW	2 cut(s)	483, 637
BsaXI	ACNNNNNCTCC	2 cut(s)	670, 700
Bsc4I	CCNNNNNNNGG	8 cut(s)	230, 355, 466, 788, 917, 1295, 1573, 1839
Bse118I	RCCGGY	1 cut(s)	1288
Bse1I	ACTGG	6 cut(s)	708, 721, 1767, 2038, 2112, 2832
Bse3DI	GCAATG	3 cut(s)	1269, 1692, 2385
Bse8I	GATNNNNATC	1 cut(s)	1539
BseAI	TCCGGA	1 cut(s)	483
BseBI	CCWGG	4 cut(s)	461, 1415, 2166, 2766
BseCI	ATCGAT	1 cut(s)	1560
BseDI	CCNNGG	4 cut(s)	393, 459, 1974, 2004
BseGI	GGATG	7 cut(s)	14, 421, 1353, 1744, 2260, 2277, 2536
BseJI	GATNNNNATC	1 cut(s)	1539
BseLI	CCNNNNNNNGG	8 cut(s)	230, 355, 466, 788, 917, 1295, 1573, 1839
BseMI	GCAATG	3 cut(s)	1269, 1692, 2385
BseMII	CTCAG	6 cut(s)	380, 723, 822, 1924, 2333, 2567
BseNI	ACTGG	6 cut(s)	708, 721, 1767, 2038, 2112, 2832
BsePI	GCGCGC	1 cut(s)	298
BseRI	GAGGAG	5 cut(s)	10, 256, 410, 413, 625
BseX3I	CGGCCG	1 cut(s)	235
Bsh1236I	CGCG	7 cut(s)	300, 302, 433, 435, 477, 1083, 1147
Bsh1285I	CGRYCG	1 cut(s)	238
BshFI	GGCC	7 cut(s)	237, 348, 470, 921, 1463, 2258, 2304
BshVI	ATCGAT	1 cut(s)	1560
BsiEI	CGRYCG	1 cut(s)	238
BsiHKAI	GWGCWC	3 cut(s)	622, 645, 1591
BsiHKCI	CYCGRG	3 cut(s)	443, 1956, 1974
BsiSI	CCGG	9 cut(s)	238, 349, 484, 638, 922, 1230, 1289, 1975, 2451
BslFI	GGGAC	8 cut(s)	190, 365, 912, 1043, 1821, 1835, 1957, 2337
BslI	CCNNNNNNNGG	8 cut(s)	230, 355, 466, 788, 917, 1295, 1573, 1839
BsmAI	GTCTC	2 cut(s)	274, 2556
BsmBI	CGTCTC	1 cut(s)	274
BsmFI	GGGAC	8 cut(s)	190, 365, 912, 1043, 1821, 1835, 1957, 2337
BsmI	GAATGC	1 cut(s)	2350
BsnI	GGCC	7 cut(s)	237, 348, 470, 921, 1463, 2258, 2304
BsoBI	CYCGRG	3 cut(s)	443, 1956, 1974
Bsp1286I	GDGCHC	3 cut(s)	622, 645, 1591
Bsp13I	TCCGGA	1 cut(s)	483
Bsp1407I	TGTACA	1 cut(s)	1171
Bsp19I	CCATGG	1 cut(s)	2004
BspANI	GGCC	7 cut(s)	237, 348, 470, 921, 1463, 2258, 2304
BspCNI	CTCAG	6 cut(s)	379, 724, 823, 1923, 2332, 2566
BspDI	ATCGAT	1 cut(s)	1560
BspEI	TCCGGA	1 cut(s)	483
BspFNI	CGCG	7 cut(s)	300, 302, 433, 435, 477, 1083, 1147
BspLI	GGNNCC	5 cut(s)	1809, 1973, 1992, 2377, 2804
BsrBI	CCGCTC	5 cut(s)	221, 286, 862, 1624, 2131
BsrDI	GCAATG	3 cut(s)	1269, 1692, 2385
BsrFI	RCCGGY	1 cut(s)	1288
BsrGI	TGTACA	1 cut(s)	1171
BsrI	ACTGG	6 cut(s)	708, 721, 1767, 2038, 2112, 2832
BssAI	RCCGGY	1 cut(s)	1288
BssECI	CCNNGG	4 cut(s)	393, 459, 1974, 2004
BssHII	GCGCGC	1 cut(s)	298
BssSI	CACGAG	1 cut(s)	885
BssT1I	CCWWGG	1 cut(s)	2004
Bst2BI	CACGAG	1 cut(s)	885
Bst2UI	CCWGG	4 cut(s)	461, 1415, 2166, 2766
Bst4CI	ACNGT	9 cut(s)	667, 1107, 1237, 1314, 1678, 1783, 1801, 2230, 2467
Bst6I	CTCTTC	2 cut(s)	1860, 2323
BstAPI	GCANNNNNTGC	3 cut(s)	376, 1829, 2834
BstAUI	TGTACA	1 cut(s)	1171
BstBAI	YACGTR	1 cut(s)	70
BstC8I	GCNNGC	5 cut(s)	300, 468, 472, 762, 2689
BstDEI	CTNAG	7 cut(s)	366, 732, 831, 1910, 2319, 2553, 2696
BstDSI	CCRYGG	1 cut(s)	2004
BstEII	GGTNACC	1 cut(s)	2157
BstENI	CCTNNNNNAGG	2 cut(s)	464, 1571
BstF5I	GGATG	7 cut(s)	14, 421, 1353, 1744, 2260, 2277, 2536
BstFNI	CGCG	7 cut(s)	300, 302, 433, 435, 477, 1083, 1147
BstH2I	RGCGCY	1 cut(s)	2688
BstHHI	GCGC	7 cut(s)	300, 302, 435, 479, 598, 1147, 2687
BstMAI	GTCTC	2 cut(s)	274, 2556
BstMCI	CGRYCG	1 cut(s)	238
BstNI	CCWGG	4 cut(s)	461, 1415, 2166, 2766
BstNSI	RCATGY	2 cut(s)	8, 2076
BstPI	GGTNACC	1 cut(s)	2157
BstSCI	CCNGG	9 cut(s)	348, 459, 921, 1413, 1973, 1974, 2164, 2450, 2764
BstUI	CGCG	7 cut(s)	300, 302, 433, 435, 477, 1083, 1147
BstV2I	GAAGAC	4 cut(s)	271, 1921, 2221, 2730
BstX2I	RGATCY	6 cut(s)	553, 791, 874, 1990, 2676, 2802
BstXI	CCANNNNNNTGG	2 cut(s)	48, 343
BstYI	RGATCY	6 cut(s)	553, 791, 874, 1990, 2676, 2802
BstZI	CGGCCG	1 cut(s)	235
Bsu15I	ATCGAT	1 cut(s)	1560
BsuI	GTATCC	1 cut(s)	919
BsuRI	GGCC	7 cut(s)	237, 348, 470, 921, 1463, 2258, 2304
BsuTUI	ATCGAT	1 cut(s)	1560
BtgI	CCRYGG	1 cut(s)	2004
BtgZI	GCGATG	1 cut(s)	395
BtrI	CACGTC	1 cut(s)	1099
BtsCI	GGATG	7 cut(s)	14, 421, 1353, 1744, 2260, 2277, 2536
BtsI	GCAGTG	3 cut(s)	879, 1866, 2772
BtsIMutI	CAGTG	6 cut(s)	879, 1103, 1319, 1866, 2772, 2839
Cac8I	GCNNGC	5 cut(s)	300, 468, 472, 762, 2689
CaiI	CAGNNNCTG	1 cut(s)	2560
CfoI	GCGC	7 cut(s)	300, 302, 435, 479, 598, 1147, 2687
Cfr10I	RCCGGY	1 cut(s)	1288
Cfr13I	GGNCC	9 cut(s)	346, 469, 691, 1462, 1699, 1808, 1971, 2027, 2257
Cfr9I	CCCGGG	1 cut(s)	1974
ClaI	ATCGAT	1 cut(s)	1560
CseI	GACGC	1 cut(s)	909
Csp6I	GTAC	6 cut(s)	1172, 1510, 1674, 2102, 2442, 2845
CspCI	CAANNNNNGTGG	2 cut(s)	2368, 2403
CviAII	CATG	8 cut(s)	5, 36, 355, 454, 1691, 2005, 2073, 2113
CviQI	GTAC	6 cut(s)	1172, 1510, 1674, 2102, 2442, 2845
DdeI	CTNAG	7 cut(s)	366, 732, 831, 1910, 2319, 2553, 2696
DrdI	GACNNNNNNGTC	1 cut(s)	2777
DriI	GACNNNNNGTC	1 cut(s)	2434
DseDI	GACNNNNNNGTC	1 cut(s)	2777
EaeI	YGGCCR	3 cut(s)	235, 919, 2302
EagI	CGGCCG	1 cut(s)	235
Eam1104I	CTCTTC	2 cut(s)	1860, 2323
Eam1105I	GACNNNNNGTC	1 cut(s)	2434
EarI	CTCTTC	2 cut(s)	1860, 2323
EciI	GGCGGA	1 cut(s)	262
Ecl136II	GAGCTC	2 cut(s)	620, 1589
EclXI	CGGCCG	1 cut(s)	235
Eco130I	CCWWGG	1 cut(s)	2004
Eco24I	GRGCYC	2 cut(s)	622, 1591
Eco32I	GATATC	2 cut(s)	33, 1541
Eco47I	GGWCC	5 cut(s)	691, 1699, 1808, 1971, 2027
Eco52I	CGGCCG	1 cut(s)	235
Eco53kI	GAGCTC	2 cut(s)	620, 1589
Eco57I	CTGAAG	2 cut(s)	951, 1841
Eco88I	CYCGRG	3 cut(s)	443, 1956, 1974
Eco91I	GGTNACC	1 cut(s)	2157
EcoICRI	GAGCTC	2 cut(s)	620, 1589
EcoNI	CCTNNNNNAGG	2 cut(s)	464, 1571
EcoO109I	RGGNCCY	2 cut(s)	691, 1971
EcoO65I	GGTNACC	1 cut(s)	2157
EcoRII	CCWGG	4 cut(s)	459, 1413, 2164, 2764
EcoRV	GATATC	2 cut(s)	33, 1541
EcoT14I	CCWWGG	1 cut(s)	2004
EcoT22I	ATGCAT	3 cut(s)	6, 1353, 1692
EcoT38I	GRGCYC	2 cut(s)	622, 1591
ErhI	CCWWGG	1 cut(s)	2004
Esp3I	CGTCTC	1 cut(s)	274
FaeI	CATG	8 cut(s)	8, 39, 358, 457, 1694, 2008, 2076, 2116
FalI	AAGNNNNNCTT	4 cut(s)	2206, 2238, 2730, 2762
FaqI	GGGAC	8 cut(s)	190, 365, 912, 1043, 1821, 1835, 1957, 2337
FatI	CATG	8 cut(s)	4, 35, 354, 453, 1690, 2004, 2072, 2112
FauI	CCCGC	4 cut(s)	428, 479, 1570, 1617
FauNDI	CATATG	2 cut(s)	2446, 2654
FblI	GTMKAC	2 cut(s)	1999, 2757
FokI	GGATG	6 cut(s)	408, 1360, 1751, 2247, 2264, 2523
FriOI	GRGCYC	2 cut(s)	622, 1591
FspBI	CTAG	5 cut(s)	549, 891, 1038, 1760, 2624
GlaI	GCGC	7 cut(s)	299, 301, 434, 478, 597, 1146, 2686
GsuI	CTGGAG	8 cut(s)	482, 608, 620, 727, 2055, 2148, 2582, 2772
HaeII	RGCGCY	1 cut(s)	2688
HaeIII	GGCC	7 cut(s)	237, 348, 470, 921, 1463, 2258, 2304
HapII	CCGG	9 cut(s)	238, 349, 484, 638, 922, 1230, 1289, 1975, 2451
HgaI	GACGC	1 cut(s)	909
HhaI	GCGC	7 cut(s)	300, 302, 435, 479, 598, 1147, 2687
Hin1II	CATG	8 cut(s)	8, 39, 358, 457, 1694, 2008, 2076, 2116
Hin6I	GCGC	7 cut(s)	298, 300, 433, 477, 596, 1145, 2685
HinP1I	GCGC	7 cut(s)	298, 300, 433, 477, 596, 1145, 2685
HincII	GTYRAC	2 cut(s)	819, 1074
HindII	GTYRAC	2 cut(s)	819, 1074
HindIII	AAGCTT	1 cut(s)	2061
HinfI	GANTC	9 cut(s)	1226, 1383, 1432, 1763, 1848, 2315, 2435, 2636, 2754
HpaII	CCGG	9 cut(s)	238, 349, 484, 638, 922, 1230, 1289, 1975, 2451
HphI	GGTGA	4 cut(s)	1476, 1638, 2151, 2780
Hpy99I	CGWCG	2 cut(s)	152, 2435
HpyAV	CCTTC	7 cut(s)	208, 1019, 1981, 2248, 2291, 2657, 2817
HpyCH4III	ACNGT	9 cut(s)	667, 1107, 1237, 1314, 1678, 1783, 1801, 2230, 2467
HpyCH4IV	ACGT	2 cut(s)	69, 1098
HpyF3I	CTNAG	7 cut(s)	366, 732, 831, 1910, 2319, 2553, 2696
HpySE526I	ACGT	2 cut(s)	69, 1098
Hsp92II	CATG	8 cut(s)	8, 39, 358, 457, 1694, 2008, 2076, 2116
HspAI	GCGC	7 cut(s)	298, 300, 433, 477, 596, 1145, 2685
Kpn2I	TCCGGA	1 cut(s)	483
LweI	GCATC	8 cut(s)	418, 833, 1331, 1338, 1360, 1411, 2547, 2782
MaeI	CTAG	5 cut(s)	549, 891, 1038, 1760, 2624
MaeII	ACGT	2 cut(s)	69, 1098
MaeIII	GTNAC	5 cut(s)	100, 1150, 1644, 2157, 2768
MbiI	CCGCTC	5 cut(s)	221, 286, 862, 1624, 2131
MflI	RGATCY	6 cut(s)	553, 791, 874, 1990, 2676, 2802
MhlI	GDGCHC	3 cut(s)	622, 645, 1591
MlsI	TGGCCA	1 cut(s)	2304
MluI	ACGCGT	1 cut(s)	1081
MluNI	TGGCCA	1 cut(s)	2304
MlyI	GAGTC	5 cut(s)	1235, 1772, 1857, 2444, 2763
MmeI	TCCRAC	5 cut(s)	985, 1119, 1219, 1411, 1960
Mox20I	TGGCCA	1 cut(s)	2304
Mph1103I	ATGCAT	3 cut(s)	6, 1353, 1692
MroI	TCCGGA	1 cut(s)	483
MroXI	GAANNNNTTC	1 cut(s)	1755
MscI	TGGCCA	1 cut(s)	2304
MseI	TTAA	8 cut(s)	696, 1109, 1160, 1605, 2150, 2340, 2714, 2878
MslI	CAYNNNNRTG	1 cut(s)	46
Msp20I	TGGCCA	1 cut(s)	2304
MspI	CCGG	9 cut(s)	238, 349, 484, 638, 922, 1230, 1289, 1975, 2451
MspR9I	CCNGG	9 cut(s)	350, 461, 923, 1415, 1975, 1976, 2166, 2452, 2766
Mva1269I	GAATGC	1 cut(s)	2350
MvaI	CCWGG	4 cut(s)	461, 1415, 2166, 2766
MvnI	CGCG	7 cut(s)	300, 302, 433, 435, 477, 1083, 1147
NciI	CCSGG	5 cut(s)	350, 923, 1975, 1976, 2452
NcoI	CCATGG	1 cut(s)	2004
NdeI	CATATG	2 cut(s)	2446, 2654
NlaIII	CATG	8 cut(s)	8, 39, 358, 457, 1694, 2008, 2076, 2116
NlaIV	GGNNCC	5 cut(s)	1809, 1973, 1992, 2377, 2804
NmeAIII	GCCGAG	1 cut(s)	418
NmuCI	GTSAC	3 cut(s)	1644, 2157, 2768
NsiI	ATGCAT	3 cut(s)	6, 1353, 1692
NspI	RCATGY	2 cut(s)	8, 2076
PaeR7I	CTCGAG	1 cut(s)	1956
PauI	GCGCGC	1 cut(s)	298
PcsI	WCGNNNNNNNCGW	2 cut(s)	273, 299
PctI	GAATGC	1 cut(s)	2350
PdmI	GAANNNNTTC	1 cut(s)	1755
PfeI	GAWTC	4 cut(s)	1383, 1432, 2315, 2636
PleI	GAGTC	5 cut(s)	1234, 1771, 1856, 2443, 2762
PpsI	GAGTC	5 cut(s)	1234, 1771, 1856, 2443, 2762
Ppu21I	YACGTR	1 cut(s)	70
PpuMI	RGGWCCY	2 cut(s)	691, 1971
PshBI	ATTAAT	2 cut(s)	2150, 2714
Psp124BI	GAGCTC	2 cut(s)	622, 1591
Psp5II	RGGWCCY	2 cut(s)	691, 1971
Psp6I	CCWGG	4 cut(s)	459, 1413, 2164, 2764
PspEI	GGTNACC	1 cut(s)	2157
PspGI	CCWGG	4 cut(s)	459, 1413, 2164, 2764
PspN4I	GGNNCC	5 cut(s)	1809, 1973, 1992, 2377, 2804
PspPI	GGNCC	9 cut(s)	346, 469, 691, 1462, 1699, 1808, 1971, 2027, 2257
PspPPI	RGGWCCY	2 cut(s)	691, 1971
PstNI	CAGNNNCTG	1 cut(s)	2560
PsuI	RGATCY	6 cut(s)	553, 791, 874, 1990, 2676, 2802
PteI	GCGCGC	1 cut(s)	298
RsaI	GTAC	6 cut(s)	1173, 1511, 1675, 2103, 2443, 2846
RsaNI	GTAC	6 cut(s)	1172, 1510, 1674, 2102, 2442, 2845
RseI	CAYNNNNRTG	1 cut(s)	46
SacI	GAGCTC	2 cut(s)	622, 1591
SaqAI	TTAA	8 cut(s)	696, 1109, 1160, 1605, 2150, 2340, 2714, 2878
Sau96I	GGNCC	9 cut(s)	346, 469, 691, 1462, 1699, 1808, 1971, 2027, 2257
ScaI	AGTACT	1 cut(s)	2846
SchI	GAGTC	5 cut(s)	1235, 1772, 1857, 2444, 2763
ScrFI	CCNGG	9 cut(s)	350, 461, 923, 1415, 1975, 1976, 2166, 2452, 2766
SduI	GDGCHC	3 cut(s)	622, 645, 1591
SfaNI	GCATC	8 cut(s)	418, 833, 1331, 1338, 1360, 1411, 2547, 2782
Sfr274I	CTCGAG	1 cut(s)	1956
SinI	GGWCC	5 cut(s)	691, 1699, 1808, 1971, 2027
SlaI	CTCGAG	1 cut(s)	1956
SmaI	CCCGGG	1 cut(s)	1976
SmiMI	CAYNNNNRTG	1 cut(s)	46
SmlI	CTYRAG	2 cut(s)	1956, 2480
SmoI	CTYRAG	2 cut(s)	1956, 2480
SpeI	ACTAGT	1 cut(s)	1037
SspMI	CTAG	5 cut(s)	549, 891, 1038, 1760, 2624
SstI	GAGCTC	2 cut(s)	622, 1591
StyD4I	CCNGG	9 cut(s)	348, 459, 921, 1413, 1973, 1974, 2164, 2450, 2764
StyI	CCWWGG	1 cut(s)	2004
TaaI	ACNGT	9 cut(s)	667, 1107, 1237, 1314, 1678, 1783, 1801, 2230, 2467
TaiI	ACGT	2 cut(s)	72, 1101
TaqI	TCGA	9 cut(s)	254, 282, 566, 608, 1560, 1706, 1957, 2250, 2398
TaqII	GACCGA	2 cut(s)	980, 1893
TatI	WGTACW	3 cut(s)	1171, 2441, 2844
TauI	GCSGC	5 cut(s)	221, 237, 477, 499, 2134
TfiI	GAWTC	4 cut(s)	1383, 1432, 2315, 2636
Tru1I	TTAA	8 cut(s)	696, 1109, 1160, 1605, 2150, 2340, 2714, 2878
Tru9I	TTAA	8 cut(s)	696, 1109, 1160, 1605, 2150, 2340, 2714, 2878
TscAI	CASTG	6 cut(s)	886, 1110, 1319, 1873, 2779, 2839
TseFI	GTSAC	3 cut(s)	1644, 2157, 2768
Tsp45I	GTSAC	3 cut(s)	1644, 2157, 2768
TspGWI	ACGGA	3 cut(s)	13, 88, 308
TspMI	CCCGGG	1 cut(s)	1974
TspRI	CASTG	6 cut(s)	886, 1110, 1319, 1873, 2779, 2839
VpaK11BI	GGWCC	5 cut(s)	691, 1699, 1808, 1971, 2027
VspI	ATTAAT	2 cut(s)	2150, 2714
XagI	CCTNNNNNAGG	2 cut(s)	464, 1571
XapI	RAATTY	1 cut(s)	1668
XbaI	TCTAGA	1 cut(s)	1759
XceI	RCATGY	2 cut(s)	8, 2076
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2845
XhoI	CTCGAG	1 cut(s)	1956
XmaI	CCCGGG	1 cut(s)	1974
XmiI	GTMKAC	2 cut(s)	1999, 2757
XmnI	GAANNNNTTC	1 cut(s)	1755
XspI	CTAG	5 cut(s)	549, 891, 1038, 1760, 2624
ZrmI	AGTACT	1 cut(s)	2846
Zsp2I	ATGCAT	3 cut(s)	6, 1353, 1692

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.