Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0013822

Physical Location & Seq

Genomic Coordinates Forward (+)

7034398 .. 7040646

Gene Structure 6249 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG095100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2988 bp

ATGCATGTGTCGTCATCCTCCTCCGTGCTGATATCATCTCCACTCCAATGGAGAACTCCATCTCAACACGTATCATTCACTCATCTTCCCTTACAATCCGTTACGACTTCCACTACTATCAGTTACGCCCGTGATCGAAGAAACGCCGTCGTTTCCCTCTACCCAATTCGCCTTATACACAACCCATTTTCTTCTTCCTTCTTGTCCCAAACCAAAGCCGCTCCCTTTCTCGGCGGCCGGAGGAGGAGAAAGAACTCGAAGCTCCAAATCGTCTCCGCCGTCTTCGAGCGGTTCACGGAGCGCGCGATCAAAGCCGTCATCTTTTCCCAGAGAGAAGCCAAAGCTCTGGGCAGGGACATGGTGTTTACTCAGCACCTCTTGCTCGGTTTGATCGCCGAGGAGGAGCAGCAGCATCGCCATCTTCATCCCAATTCGCGCGGGTTTCTCGGGTCGGGCATGACCCTGGAACAGGCCCGCCGCGCCGTCCGGAGCATTTGGCGGCATAATAGTCATAGTCAAAGTCAAAGTCAAGGTCAAGCTGCTTCTGCTGGCTCTGGTTCGGGTTCGGGTTCTGCTAGGGATCTCTCGTTTTCGATTAGCACAAAGAGAGTTCTGGAGGCTGCGCTGGAGTATTCGAGGAGTAGAGCTCATAATTTTATTGCTCCGGAGCACATTGTCATCGGTTTATTGACCGTTGATGATGGAAGTGCTGGTCAGGTTCTTAAAAGACTGGGGGTAAATGTAAACCAGTTGTTAGCTGAGGCAGCCTCCAGACTTCAAATAGAGCTTGCCAAAGATGGTAGAGAGCCATCTGGGGGATCTCAAAAGACATTTTCTAAAAAGTCAACTGCATCGTCTGAGAAAACCAAAGAAAAAAGTGCTTTGGAGCGGTTCTGTGTGGATCTCACTGCTCGTGCTAGTGAGGGACGCATTGATCCTGTTATTGGCCGGGATACTGAAGTTCAGAGAATTATTCAGATACTTTGTCGCAGAACTAAAAATAATCCCATTCTTCTCGGTCAAAGTGGGGTTGGAAAGACAGCCATTGTTGAAGGTCTGGCAACTAGTATTGCACAGGCAGATGTCCCTGTTTATCTGTTGACAAAACGCGTAATGTCTTTGGACGTGGCACTGTTAATGGCTGGTGCAAAGGAGAGGGGAGAGTTGGAGGCGCGTGTTACCTCCTTAATAAGTGATGTACAAAAATCAGGAAATGTTATTCTGTTTATTGATGAAGTTCATACCCTTATTGAGTCCGGCACAGTTGGACGAGGAAATAAGGGGTCTGGTCTTGACATTGCTAATATAATGAAACCGGCACTTGGGAGGGGTAAATTACAGTGTATTGCATCAACTACTACCGATGAATACAGGATGCATCTTGAAAAGGATAAAGCATTTGGACGAAGATTCCAACCTGTCTGGATCAATGAACCAAGCCAGGATGATGCAGTTAGGATTCTGTTTGGTTTGCGTGAGAAATATGAGGCCCATCACAACTGCATATATGCACCAGAAGCCATTAGTGCTGCTGTGCACCTGTCAGCAAGATATATTCCTGATAGATATCTTCCTGATAAAGCTATCGATCTCCTTGATGAGGCGGGAAGTAGAGCTCGTATGGAAGCCTTTAAGAAGAAAAAAGAAGAGCGGGTTGGCATACTTTCAAAGTCACCTGATGATTATTGGCAAGAAATTCGTACAGTTCAGGCAATGCATGAAGTGGTCCTATCGAGTGAACTGAAAAATGGAGCTGCTTCTGTGGATGATACCAGAGAACATATTCTAGAGTCCATTTCATCTTCCACAGTAGATGATGAACCTACTGTGGTGGGACCAAATGATATAGCAGCAGTTGCCTCACTATGGTCAGGAGTCCCTCTTCAGCAGCTCACTGCTGATGACCGACTGCTTTTGGTGGGTCTTGATGAGAAGCTCAGAAGACGGGTTGTTGGTCAAGATGAGGCTGTTGCAGCCATTTCTCGAGCTGTTAGGAGGTCCCGGGTTGGATTGAAGGATCCAAGTAGACCCATGGCAACAATGCTCTTTTGCGGTCCTACTGGAGTTGGCAAAACTGAACTGACAAAAGCTTTGTCTGCATGTTATTTTGGATCGGAGGAAGCTATGGTACGACTGGACATGAGTGAATATATGGAGCGGCACTCTGTCAGCAAATTAATAGGGTCACCCCCAGGCTATGTTGGCTTTGGAGAGGGAGGCACTCTAACAGAAGCTATCAGAAGACGACCTTTTACAGTAGTTGTGCTTGATGAAATCGAGAAGGCCCATCCAGATATATTCAACATCCTTCTCCAAATCTTTGAAGATGGCCACCTTACTGATTCTCAGGGACGAAGAGTATCGTTTAAGAATGCTTTGGTAGTGATGACATCTAATGTGGGTTCCACTATCATTGCAAAGGGTCGAAAAAGCTCCATTGGTTTTGTGCTTACAGACGATGAGTCAAGTACATATGCCGGGATAAAAGCAACAGTAATGGAAGAACTCAAGTCATATTTTCGTCCAGAGTTGCTCAACAGGATAGACGAAGTAGTTGTATTCCATCCCCTTGAAAAGTCTCAGATGCTGGAGATTGTAAATATAATGCTACAAGAAGTGAAGCAGAGGCTAATATCTCTGGGAATTGGACTAGAGGTGTCTGAATCAGTCAAGAACCTCATATGCAAAGAAGGCTATGATCGGATCTATGGTGCTCGCCCTCTTAGACGAGCAATTACATTAATAATTGAAGACCCATTGAGTGAAGCCCTCCTTGCTGGAGTTTACCAGCCTGGTGACACTGCCGTCATTGATTTAGATGCTTCTGGGAACCCTTCTGTAAGTAATGGATCAGAGAGAAAAGAACAGTTAAAAGAGAAAGCCACCGCCTTTTTCCGGTTAGTCCAAAAACAAAGCTGGGCCGGCCTCCTATTTGATGTGCACTCCACTGCTTCTTCTCGATTCCAGCGATGCAATGGACGCCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009941 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022607 GO:0031967 GO:0031975 GO:0033554 GO:0034214 GO:0034605 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046983 GO:0047484 GO:0048518 GO:0048583 GO:0048584 GO:0050789 GO:0050896 GO:0051259 GO:0051716 GO:0065003 GO:0065007 GO:0071840 GO:0080134 GO:1901000 GO:1901002 GO:1902584 GO:2000070

KEGG Pathways

Metabolic & Signaling

ko01100 map01100

Pfam Domains

Protein Families

AAA AAA_2 ClpB_D2-small Clp_N

Protein Analysis

995

Amino Acids

109.54

Weight (kDa)

9.18

Isoelectric Point (pI)

51.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	197 - 267	3e-11	Clp repeat (R) N-terminal domain
AAA_16	PF13191	314 - 367	8.7e-06	AAA ATPase domain
NBD_SMAX1	PF23569	320 - 421	6.1e-06	SMAX1 nucleotide binding domain
AAA	PF00004	337 - 453	6.2e-09	ATPase family associated with various cellular activities (AAA)
AAA_lid_9	PF17871	480 - 556	9.5e-27	AAA lid domain
AAA_2	PF07724	676 - 851	3.2e-63	AAA domain (Cdc48 subfamily)
AAA_5	PF07728	681 - 801	8.2e-11	AAA domain (dynein-related subfamily)
ClpB_D2-small	PF10431	857 - 936	3e-23	C-terminal, D2-small domain, of ClpB protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0013822)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51070
fragaria_vesca	FvH4_5g10020
malus_domestica	MD06G1128400.v1.1
prunus_persica	Prupe.5G138200_v2.0.a1
pyrus_communis	pycom06g11860
rosa_chinensis	RchiOBHm_Chr7g0188241
rosa_laevigata	RLG00000004715
rosa_multiflora	Rmu_co8357845.1_g000001 Rmu_co8388145.1_g000001 Rmu_sc0008750.1_g000013
rosa_roxburghii	Rroxscaffold_3G00266580
rosa_rugosa	Rorug06G0489400
rosa_samantha	Rh7AG093700 Rh7BG095100 Rh7CG095500 Rh7DG095700
rosa_wichuraiana	Rw7G008070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2804
AccBSI	CCGCTC	5 cut(s)	221, 289, 889, 1651, 2158
AccI	GTMKAC	1 cut(s)	2026
AccII	CGCG	7 cut(s)	303, 305, 436, 438, 480, 1110, 1174
AccIII	TCCGGA	2 cut(s)	486, 664
AcoI	YGGCCR	3 cut(s)	235, 946, 2329
AcsI	RAATTY	1 cut(s)	1695
AcuI	CTGAAG	2 cut(s)	978, 1868
AcyI	GRCGYC	1 cut(s)	2980
AfaI	GTAC	4 cut(s)	1200, 1702, 2130, 2470
AfiI	CCNNNNNNNGG	8 cut(s)	230, 469, 815, 944, 1322, 1600, 1866, 2895
AflIII	ACRYGT	2 cut(s)	67, 1108
AgsI	TTSAA	9 cut(s)	779, 1052, 1385, 1668, 2014, 2302, 2324, 2573, 2750
AhdI	GACNNNNNGTC	1 cut(s)	2461
AhlI	ACTAGT	1 cut(s)	1064
AjiI	CACGTC	1 cut(s)	1126
AjnI	CCWGG	4 cut(s)	462, 1440, 2191, 2791
AjuI	GAANNNNNNNTTGG	2 cut(s)	1638, 1670
Alw21I	GWGCWC	6 cut(s)	649, 672, 1539, 1618, 2716, 2943
Alw26I	GTCTC	2 cut(s)	277, 2583
Alw44I	GTGCAC	2 cut(s)	1535, 2939
AlwNI	CAGNNNCTG	1 cut(s)	2587
Ama87I	CYCGRG	3 cut(s)	446, 1983, 2001
Aor13HI	TCCGGA	2 cut(s)	486, 664
AoxI	GGCC	8 cut(s)	235, 471, 946, 1488, 2283, 2329, 2919, 2923
ApaLI	GTGCAC	2 cut(s)	1535, 2939
ApoI	RAATTY	1 cut(s)	1695
ArsI	GACNNNNNNTTYG	8 cut(s)	511, 517, 543, 549, 2074, 2078, 2106, 2110
AseI	ATTAAT	2 cut(s)	2177, 2741
Asp700I	GAANNNNTTC	1 cut(s)	1782
AspLEI	GCGC	6 cut(s)	303, 305, 438, 482, 625, 1174
AspS9I	GGNCC	8 cut(s)	472, 1489, 1726, 1835, 1998, 2054, 2284, 2919
AsuC2I	CCSGG	4 cut(s)	950, 2002, 2003, 2479
AsuHPI	GGTGA	3 cut(s)	1665, 2178, 2807
AvaI	CYCGRG	3 cut(s)	446, 1983, 2001
AvaII	GGWCC	4 cut(s)	1726, 1835, 1998, 2054
BaeGI	GKGCMC	2 cut(s)	1539, 2943
BaeI	ACNNNNGTAYC	2 cut(s)	945, 978
BalI	TGGCCA	1 cut(s)	2331
BamHI	GGATCC	1 cut(s)	2017
BanII	GRGCYC	2 cut(s)	649, 1618
BarI	GAAGNNNNNNTAC	2 cut(s)	2112, 2144
BauI	CACGAG	1 cut(s)	912
BbsI	GAAGAC	4 cut(s)	274, 1948, 2248, 2757
Bbv12I	GWGCWC	6 cut(s)	649, 672, 1539, 1618, 2716, 2943
BbvCI	CCTCAGC	1 cut(s)	759
BccI	CCATC	9 cut(s)	67, 426, 695, 791, 817, 1500, 2295, 2321, 2571
BceAI	ACGGC	6 cut(s)	131, 263, 299, 467, 2789, 2967
BcgI	CGANNNNNNTGC	4 cut(s)	395, 429, 1679, 1713
BciT130I	CCWGG	4 cut(s)	464, 1442, 2193, 2793
BciVI	GTATCC	1 cut(s)	946
BcnI	CCSGG	4 cut(s)	950, 2002, 2003, 2479
BcoDI	GTCTC	2 cut(s)	277, 2583
BcuI	ACTAGT	1 cut(s)	1064
BfaI	CTAG	5 cut(s)	576, 918, 1065, 1787, 2651
BfuI	GTATCC	1 cut(s)	946
Bme1390I	CCNGG	8 cut(s)	464, 950, 1442, 2002, 2003, 2193, 2479, 2793
Bme18I	GGWCC	4 cut(s)	1726, 1835, 1998, 2054
BmeRI	GACNNNNNGTC	1 cut(s)	2461
BmeT110I	CYCGRG	3 cut(s)	446, 1983, 2001
BmgBI	CACGTC	1 cut(s)	1126
BmgT120I	GGNCC	8 cut(s)	472, 1489, 1726, 1835, 1998, 2054, 2284, 2919
BmiI	GGNNCC	5 cut(s)	1836, 2000, 2019, 2404, 2831
BmrFI	CCNGG	8 cut(s)	464, 950, 1442, 2002, 2003, 2193, 2479, 2793
BmrI	ACTGGG	1 cut(s)	740
BmsI	GCATC	9 cut(s)	421, 860, 1358, 1365, 1387, 1438, 2574, 2809, 2960
BmuI	ACTGGG	1 cut(s)	740
BpiI	GAAGAC	4 cut(s)	274, 1948, 2248, 2757
BplI	GAGNNNNNCTC	6 cut(s)	631, 663, 752, 784, 2147, 2179
BpmI	CTGGAG	6 cut(s)	635, 647, 754, 2082, 2609, 2799
Bpu10I	CCTNAGC	1 cut(s)	759
BpuEI	CTTGAG	1 cut(s)	2492
BpuMI	CCSGG	4 cut(s)	950, 2002, 2003, 2479
Bsa29I	ATCGAT	1 cut(s)	1587
BsaAI	YACGTR	1 cut(s)	70
BsaBI	GATNNNNATC	1 cut(s)	1566
BsaHI	GRCGYC	1 cut(s)	2980
BsaJI	CCNNGG	5 cut(s)	396, 462, 2001, 2031, 2191
BsaWI	WCCGGW	3 cut(s)	486, 664, 2895
Bsc4I	CCNNNNNNNGG	8 cut(s)	230, 469, 815, 944, 1322, 1600, 1866, 2895
Bse118I	RCCGGY	2 cut(s)	1315, 2921
Bse1I	ACTGG	4 cut(s)	735, 748, 2065, 2139
Bse3DI	GCAATG	4 cut(s)	1296, 1719, 2412, 2980
Bse8I	GATNNNNATC	1 cut(s)	1566
BseAI	TCCGGA	2 cut(s)	486, 664
BseBI	CCWGG	4 cut(s)	464, 1442, 2193, 2793
BseCI	ATCGAT	1 cut(s)	1587
BseDI	CCNNGG	5 cut(s)	396, 462, 2001, 2031, 2191
BseGI	GGATG	8 cut(s)	14, 424, 1380, 1450, 1771, 2287, 2304, 2563
BseJI	GATNNNNATC	1 cut(s)	1566
BseLI	CCNNNNNNNGG	8 cut(s)	230, 469, 815, 944, 1322, 1600, 1866, 2895
BseMI	GCAATG	4 cut(s)	1296, 1719, 2412, 2980
BseMII	CTCAG	6 cut(s)	383, 750, 849, 1951, 2360, 2594
BseNI	ACTGG	4 cut(s)	735, 748, 2065, 2139
BsePI	GCGCGC	1 cut(s)	301
BseRI	GAGGAG	6 cut(s)	10, 256, 259, 413, 416, 652
BseSI	GKGCMC	2 cut(s)	1539, 2943
BseX3I	CGGCCG	1 cut(s)	235
BseYI	CCCAGC	1 cut(s)	2916
Bsh1236I	CGCG	7 cut(s)	303, 305, 436, 438, 480, 1110, 1174
Bsh1285I	CGRYCG	1 cut(s)	238
BshFI	GGCC	8 cut(s)	237, 473, 948, 1490, 2285, 2331, 2921, 2925
BshVI	ATCGAT	1 cut(s)	1587
BsiEI	CGRYCG	1 cut(s)	238
BsiHKAI	GWGCWC	6 cut(s)	649, 672, 1539, 1618, 2716, 2943
BsiHKCI	CYCGRG	3 cut(s)	446, 1983, 2001
BslFI	GGGAC	8 cut(s)	190, 368, 939, 1070, 1848, 1862, 1984, 2364
BslI	CCNNNNNNNGG	8 cut(s)	230, 469, 815, 944, 1322, 1600, 1866, 2895
BsmAI	GTCTC	2 cut(s)	277, 2583
BsmBI	CGTCTC	1 cut(s)	277
BsmFI	GGGAC	8 cut(s)	190, 368, 939, 1070, 1848, 1862, 1984, 2364
BsmI	GAATGC	1 cut(s)	2377
BsnI	GGCC	8 cut(s)	237, 473, 948, 1490, 2285, 2331, 2921, 2925
BsoBI	CYCGRG	3 cut(s)	446, 1983, 2001
Bsp1286I	GDGCHC	6 cut(s)	649, 672, 1539, 1618, 2716, 2943
Bsp13I	TCCGGA	2 cut(s)	486, 664
Bsp1407I	TGTACA	1 cut(s)	1198
Bsp19I	CCATGG	1 cut(s)	2031
BspANI	GGCC	8 cut(s)	237, 473, 948, 1490, 2285, 2331, 2921, 2925
BspCNI	CTCAG	6 cut(s)	382, 751, 850, 1950, 2359, 2593
BspDI	ATCGAT	1 cut(s)	1587
BspEI	TCCGGA	2 cut(s)	486, 664
BspFNI	CGCG	7 cut(s)	303, 305, 436, 438, 480, 1110, 1174
BspLI	GGNNCC	5 cut(s)	1836, 2000, 2019, 2404, 2831
BspQI	GCTCTTC	1 cut(s)	1641
BsrBI	CCGCTC	5 cut(s)	221, 289, 889, 1651, 2158
BsrDI	GCAATG	4 cut(s)	1296, 1719, 2412, 2980
BsrFI	RCCGGY	2 cut(s)	1315, 2921
BsrGI	TGTACA	1 cut(s)	1198
BsrI	ACTGG	4 cut(s)	735, 748, 2065, 2139
BssAI	RCCGGY	2 cut(s)	1315, 2921
BssECI	CCNNGG	5 cut(s)	396, 462, 2001, 2031, 2191
BssHII	GCGCGC	1 cut(s)	301
BssNI	GRCGYC	1 cut(s)	2980
BssSI	CACGAG	1 cut(s)	912
BssT1I	CCWWGG	1 cut(s)	2031
Bst2BI	CACGAG	1 cut(s)	912
Bst2UI	CCWGG	4 cut(s)	464, 1442, 2193, 2793
Bst6I	CTCTTC	3 cut(s)	1641, 1887, 2350
BstACI	GRCGYC	1 cut(s)	2980
BstAPI	GCANNNNNTGC	2 cut(s)	379, 1856
BstAUI	TGTACA	1 cut(s)	1198
BstBAI	YACGTR	1 cut(s)	70
BstC8I	GCNNGC	6 cut(s)	303, 475, 550, 789, 2716, 2923
BstDEI	CTNAG	7 cut(s)	369, 759, 858, 1937, 2346, 2580, 2723
BstDSI	CCRYGG	1 cut(s)	2031
BstEII	GGTNACC	1 cut(s)	2184
BstENI	CCTNNNNNAGG	2 cut(s)	467, 1598
BstF5I	GGATG	8 cut(s)	14, 424, 1380, 1450, 1771, 2287, 2304, 2563
BstFNI	CGCG	7 cut(s)	303, 305, 436, 438, 480, 1110, 1174
BstHHI	GCGC	6 cut(s)	303, 305, 438, 482, 625, 1174
BstMAI	GTCTC	2 cut(s)	277, 2583
BstMCI	CGRYCG	1 cut(s)	238
BstNI	CCWGG	4 cut(s)	464, 1442, 2193, 2793
BstNSI	RCATGY	2 cut(s)	8, 2103
BstPI	GGTNACC	1 cut(s)	2184
BstSCI	CCNGG	8 cut(s)	462, 948, 1440, 2000, 2001, 2191, 2477, 2791
BstSLI	GKGCMC	2 cut(s)	1539, 2943
BstUI	CGCG	7 cut(s)	303, 305, 436, 438, 480, 1110, 1174
BstV2I	GAAGAC	4 cut(s)	274, 1948, 2248, 2757
BstX2I	RGATCY	5 cut(s)	580, 818, 901, 2017, 2703
BstXI	CCANNNNNNTGG	2 cut(s)	48, 346
BstYI	RGATCY	5 cut(s)	580, 818, 901, 2017, 2703
BstZI	CGGCCG	1 cut(s)	235
Bsu15I	ATCGAT	1 cut(s)	1587
BsuI	GTATCC	1 cut(s)	946
BsuRI	GGCC	8 cut(s)	237, 473, 948, 1490, 2285, 2331, 2921, 2925
BsuTUI	ATCGAT	1 cut(s)	1587
BtgI	CCRYGG	1 cut(s)	2031
BtgZI	GCGATG	2 cut(s)	398, 2983
BtrI	CACGTC	1 cut(s)	1126
BtsCI	GGATG	8 cut(s)	14, 424, 1380, 1450, 1771, 2287, 2304, 2563
BtsI	GCAGTG	4 cut(s)	906, 1893, 2799, 2946
BtsIMutI	CAGTG	6 cut(s)	906, 1130, 1346, 1893, 2799, 2946
Cac8I	GCNNGC	6 cut(s)	303, 475, 550, 789, 2716, 2923
CaiI	CAGNNNCTG	1 cut(s)	2587
CfoI	GCGC	6 cut(s)	303, 305, 438, 482, 625, 1174
Cfr10I	RCCGGY	2 cut(s)	1315, 2921
Cfr13I	GGNCC	8 cut(s)	472, 1489, 1726, 1835, 1998, 2054, 2284, 2919
Cfr9I	CCCGGG	1 cut(s)	2001
ClaI	ATCGAT	1 cut(s)	1587
CseI	GACGC	1 cut(s)	936
Csp6I	GTAC	4 cut(s)	1199, 1701, 2129, 2469
CspCI	CAANNNNNGTGG	2 cut(s)	2395, 2430
CviAII	CATG	7 cut(s)	5, 358, 457, 1718, 2032, 2100, 2140
CviQI	GTAC	4 cut(s)	1199, 1701, 2129, 2469
DdeI	CTNAG	7 cut(s)	369, 759, 858, 1937, 2346, 2580, 2723
DrdI	GACNNNNNNGTC	1 cut(s)	2804
DriI	GACNNNNNGTC	1 cut(s)	2461
DseDI	GACNNNNNNGTC	1 cut(s)	2804
EaeI	YGGCCR	3 cut(s)	235, 946, 2329
EagI	CGGCCG	1 cut(s)	235
Eam1104I	CTCTTC	3 cut(s)	1641, 1887, 2350
Eam1105I	GACNNNNNGTC	1 cut(s)	2461
EarI	CTCTTC	3 cut(s)	1641, 1887, 2350
EciI	GGCGGA	1 cut(s)	265
Ecl136II	GAGCTC	2 cut(s)	647, 1616
EclXI	CGGCCG	1 cut(s)	235
Eco130I	CCWWGG	1 cut(s)	2031
Eco24I	GRGCYC	2 cut(s)	649, 1618
Eco32I	GATATC	2 cut(s)	33, 1568
Eco47I	GGWCC	4 cut(s)	1726, 1835, 1998, 2054
Eco52I	CGGCCG	1 cut(s)	235
Eco53kI	GAGCTC	2 cut(s)	647, 1616
Eco57I	CTGAAG	2 cut(s)	978, 1868
Eco88I	CYCGRG	3 cut(s)	446, 1983, 2001
Eco91I	GGTNACC	1 cut(s)	2184
EcoICRI	GAGCTC	2 cut(s)	647, 1616
EcoNI	CCTNNNNNAGG	2 cut(s)	467, 1598
EcoO109I	RGGNCCY	1 cut(s)	1998
EcoO65I	GGTNACC	1 cut(s)	2184
EcoRII	CCWGG	4 cut(s)	462, 1440, 2191, 2791
EcoRV	GATATC	2 cut(s)	33, 1568
EcoT14I	CCWWGG	1 cut(s)	2031
EcoT22I	ATGCAT	3 cut(s)	6, 1380, 1719
EcoT38I	GRGCYC	2 cut(s)	649, 1618
ErhI	CCWWGG	1 cut(s)	2031
Esp3I	CGTCTC	1 cut(s)	277
FaeI	CATG	7 cut(s)	8, 361, 460, 1721, 2035, 2103, 2143
FalI	AAGNNNNNCTT	4 cut(s)	2233, 2265, 2757, 2789
FaqI	GGGAC	8 cut(s)	190, 368, 939, 1070, 1848, 1862, 1984, 2364
FatI	CATG	7 cut(s)	4, 357, 456, 1717, 2031, 2099, 2139
FauI	CCCGC	4 cut(s)	431, 482, 1597, 1644
FauNDI	CATATG	2 cut(s)	2473, 2681
FblI	GTMKAC	1 cut(s)	2026
FokI	GGATG	7 cut(s)	411, 1387, 1457, 1778, 2274, 2291, 2550
FriOI	GRGCYC	2 cut(s)	649, 1618
FseI	GGCCGGCC	1 cut(s)	2925
FspBI	CTAG	5 cut(s)	576, 918, 1065, 1787, 2651
GlaI	GCGC	6 cut(s)	302, 304, 437, 481, 624, 1173
GsaI	CCCAGC	1 cut(s)	2920
GsuI	CTGGAG	6 cut(s)	635, 647, 754, 2082, 2609, 2799
HaeIII	GGCC	8 cut(s)	237, 473, 948, 1490, 2285, 2331, 2921, 2925
HgaI	GACGC	1 cut(s)	936
HhaI	GCGC	6 cut(s)	303, 305, 438, 482, 625, 1174
Hin1I	GRCGYC	1 cut(s)	2980
Hin1II	CATG	7 cut(s)	8, 361, 460, 1721, 2035, 2103, 2143
Hin6I	GCGC	6 cut(s)	301, 303, 436, 480, 623, 1172
HinP1I	GCGC	6 cut(s)	301, 303, 436, 480, 623, 1172
HincII	GTYRAC	2 cut(s)	846, 1101
HindII	GTYRAC	2 cut(s)	846, 1101
HindIII	AAGCTT	1 cut(s)	2088
HinfI	GANTC	9 cut(s)	1253, 1410, 1459, 1790, 1875, 2342, 2462, 2663, 2961
HphI	GGTGA	3 cut(s)	1665, 2178, 2807
Hpy99I	CGWCG	1 cut(s)	152
HpyAV	CCTTC	7 cut(s)	208, 1046, 2008, 2275, 2318, 2684, 2844
HpyCH4IV	ACGT	2 cut(s)	69, 1125
HpyF3I	CTNAG	7 cut(s)	369, 759, 858, 1937, 2346, 2580, 2723
HpySE526I	ACGT	2 cut(s)	69, 1125
Hsp92I	GRCGYC	1 cut(s)	2980
Hsp92II	CATG	7 cut(s)	8, 361, 460, 1721, 2035, 2103, 2143
HspAI	GCGC	6 cut(s)	301, 303, 436, 480, 623, 1172
Kpn2I	TCCGGA	2 cut(s)	486, 664
KroI	GCCGGC	1 cut(s)	2921
KroNI	GCCGGC	1 cut(s)	2923
LguI	GCTCTTC	1 cut(s)	1641
LweI	GCATC	9 cut(s)	421, 860, 1358, 1365, 1387, 1438, 2574, 2809, 2960
MaeI	CTAG	5 cut(s)	576, 918, 1065, 1787, 2651
MaeII	ACGT	2 cut(s)	69, 1125
MaeIII	GTNAC	6 cut(s)	100, 122, 1177, 1671, 2184, 2795
MbiI	CCGCTC	5 cut(s)	221, 289, 889, 1651, 2158
MflI	RGATCY	5 cut(s)	580, 818, 901, 2017, 2703
MhlI	GDGCHC	6 cut(s)	649, 672, 1539, 1618, 2716, 2943
MlsI	TGGCCA	1 cut(s)	2331
MluI	ACGCGT	1 cut(s)	1108
MluNI	TGGCCA	1 cut(s)	2331
MlyI	GAGTC	4 cut(s)	1262, 1799, 1884, 2471
MmeI	TCCRAC	5 cut(s)	1012, 1146, 1246, 1438, 1987
Mox20I	TGGCCA	1 cut(s)	2331
Mph1103I	ATGCAT	3 cut(s)	6, 1380, 1719
MroI	TCCGGA	2 cut(s)	486, 664
MroNI	GCCGGC	1 cut(s)	2921
MroXI	GAANNNNTTC	1 cut(s)	1782
MscI	TGGCCA	1 cut(s)	2331
MseI	TTAA	8 cut(s)	723, 1136, 1187, 1632, 2177, 2367, 2741, 2870
MslI	CAYNNNNRTG	1 cut(s)	46
Msp20I	TGGCCA	1 cut(s)	2331
MspR9I	CCNGG	8 cut(s)	464, 950, 1442, 2002, 2003, 2193, 2479, 2793
Mva1269I	GAATGC	1 cut(s)	2377
MvaI	CCWGG	4 cut(s)	464, 1442, 2193, 2793
MvnI	CGCG	7 cut(s)	303, 305, 436, 438, 480, 1110, 1174
NaeI	GCCGGC	1 cut(s)	2923
NciI	CCSGG	4 cut(s)	950, 2002, 2003, 2479
NcoI	CCATGG	1 cut(s)	2031
NdeI	CATATG	2 cut(s)	2473, 2681
NgoMIV	GCCGGC	1 cut(s)	2921
NlaIII	CATG	7 cut(s)	8, 361, 460, 1721, 2035, 2103, 2143
NlaIV	GGNNCC	5 cut(s)	1836, 2000, 2019, 2404, 2831
NmeAIII	GCCGAG	2 cut(s)	210, 421
NmuCI	GTSAC	3 cut(s)	1671, 2184, 2795
NsiI	ATGCAT	3 cut(s)	6, 1380, 1719
NspI	RCATGY	2 cut(s)	8, 2103
PaeR7I	CTCGAG	1 cut(s)	1983
PauI	GCGCGC	1 cut(s)	301
PciSI	GCTCTTC	1 cut(s)	1641
PcsI	WCGNNNNNNNCGW	3 cut(s)	276, 302, 2965
PctI	GAATGC	1 cut(s)	2377
PdiI	GCCGGC	1 cut(s)	2923
PdmI	GAANNNNTTC	1 cut(s)	1782
PfeI	GAWTC	5 cut(s)	1410, 1459, 2342, 2663, 2961
PleI	GAGTC	4 cut(s)	1261, 1798, 1883, 2470
PpsI	GAGTC	4 cut(s)	1261, 1798, 1883, 2470
Ppu21I	YACGTR	1 cut(s)	70
PpuMI	RGGWCCY	1 cut(s)	1998
PshBI	ATTAAT	2 cut(s)	2177, 2741
Psp124BI	GAGCTC	2 cut(s)	649, 1618
Psp5II	RGGWCCY	1 cut(s)	1998
Psp6I	CCWGG	4 cut(s)	462, 1440, 2191, 2791
PspEI	GGTNACC	1 cut(s)	2184
PspFI	CCCAGC	1 cut(s)	2916
PspGI	CCWGG	4 cut(s)	462, 1440, 2191, 2791
PspN4I	GGNNCC	5 cut(s)	1836, 2000, 2019, 2404, 2831
PspPI	GGNCC	8 cut(s)	472, 1489, 1726, 1835, 1998, 2054, 2284, 2919
PspPPI	RGGWCCY	1 cut(s)	1998
PstNI	CAGNNNCTG	1 cut(s)	2587
PsuI	RGATCY	5 cut(s)	580, 818, 901, 2017, 2703
PteI	GCGCGC	1 cut(s)	301
RigI	GGCCGGCC	1 cut(s)	2925
RsaI	GTAC	4 cut(s)	1200, 1702, 2130, 2470
RsaNI	GTAC	4 cut(s)	1199, 1701, 2129, 2469
RseI	CAYNNNNRTG	1 cut(s)	46
SacI	GAGCTC	2 cut(s)	649, 1618
SapI	GCTCTTC	1 cut(s)	1641
SaqAI	TTAA	8 cut(s)	723, 1136, 1187, 1632, 2177, 2367, 2741, 2870
Sau96I	GGNCC	8 cut(s)	472, 1489, 1726, 1835, 1998, 2054, 2284, 2919
SchI	GAGTC	4 cut(s)	1262, 1799, 1884, 2471
ScrFI	CCNGG	8 cut(s)	464, 950, 1442, 2002, 2003, 2193, 2479, 2793
SduI	GDGCHC	6 cut(s)	649, 672, 1539, 1618, 2716, 2943
SfaNI	GCATC	9 cut(s)	421, 860, 1358, 1365, 1387, 1438, 2574, 2809, 2960
Sfr274I	CTCGAG	1 cut(s)	1983
SinI	GGWCC	4 cut(s)	1726, 1835, 1998, 2054
SlaI	CTCGAG	1 cut(s)	1983
SmaI	CCCGGG	1 cut(s)	2003
SmiMI	CAYNNNNRTG	1 cut(s)	46
SmlI	CTYRAG	2 cut(s)	1983, 2507
SmoI	CTYRAG	2 cut(s)	1983, 2507
SpeI	ACTAGT	1 cut(s)	1064
SspMI	CTAG	5 cut(s)	576, 918, 1065, 1787, 2651
SstI	GAGCTC	2 cut(s)	649, 1618
StyD4I	CCNGG	8 cut(s)	462, 948, 1440, 2000, 2001, 2191, 2477, 2791
StyI	CCWWGG	1 cut(s)	2031
TaiI	ACGT	2 cut(s)	72, 1128
TaqII	GACCGA	2 cut(s)	1007, 1920
TatI	WGTACW	2 cut(s)	1198, 2468
TauI	GCSGC	5 cut(s)	221, 237, 480, 502, 2161
TfiI	GAWTC	5 cut(s)	1410, 1459, 2342, 2663, 2961
Tru1I	TTAA	8 cut(s)	723, 1136, 1187, 1632, 2177, 2367, 2741, 2870
Tru9I	TTAA	8 cut(s)	723, 1136, 1187, 1632, 2177, 2367, 2741, 2870
TscAI	CASTG	6 cut(s)	913, 1137, 1346, 1900, 2806, 2953
TseFI	GTSAC	3 cut(s)	1671, 2184, 2795
Tsp45I	GTSAC	3 cut(s)	1671, 2184, 2795
TspGWI	ACGGA	3 cut(s)	13, 88, 311
TspMI	CCCGGG	1 cut(s)	2001
TspRI	CASTG	6 cut(s)	913, 1137, 1346, 1900, 2806, 2953
VneI	GTGCAC	2 cut(s)	1535, 2939
VpaK11BI	GGWCC	4 cut(s)	1726, 1835, 1998, 2054
VspI	ATTAAT	2 cut(s)	2177, 2741
XagI	CCTNNNNNAGG	2 cut(s)	467, 1598
XapI	RAATTY	1 cut(s)	1695
XbaI	TCTAGA	1 cut(s)	1786
XceI	RCATGY	2 cut(s)	8, 2103
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2972
XhoI	CTCGAG	1 cut(s)	1983
XmaI	CCCGGG	1 cut(s)	2001
XmiI	GTMKAC	1 cut(s)	2026
XmnI	GAANNNNTTC	1 cut(s)	1782
XspI	CTAG	5 cut(s)	576, 918, 1065, 1787, 2651
Zsp2I	ATGCAT	3 cut(s)	6, 1380, 1719

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.