Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0013822

Physical Location & Seq

Genomic Coordinates Reverse (-)

61640176 .. 61644133

Gene Structure 3958 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004715

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2127 bp

ATGCATGTGTCGTCATCCTCCTCCGTGCTGATATCATCTCCACTCCAATGGAGAACTCCATCTCAACACGTATCATTCACTCATCTTCCCGTACAATCCGTTACGACTTCCACTACTATCAGTTACGCCCGTGATCGCAGAAACGCCGTCGTTTCCCTCTACCCAATTCGCCTTATACACAACCCATTTTCTTCTTCCTTCTTGTCCCAAACCAAAGCCGCTCCCTTTCACGGCGACCGGAGGAGAAAGAACTCGAAGCTCCAAATCGTCTCCGCCGTCTTCGAGCGGTTCACGGAGCGCGCGATCAAAGCCGTCATCTTTTCCCAGAGAGAAGCCAAAGCTCTGGGCCGGGACATGGTGTTTACTCAGCACCTTTTGCTCGGTTTGATCGCCGAGGAGGAGCAGCAGCATCGCCATCTTCATCCGAATTCGCGCGGGTTTCTCGGGTCGGGCATGACCCTGGAGCAGGCCCGCCGCGCCGTCCGGAGCATTTGGCGGCATAATAGTCAAAGTCAAAGTCAAGGTCAAGCTGCTGGCTCTGGTCCGGGTTCTGCTAGGGATCTCTCGTTTTCGATTAGCACAAAGAGAGTTCTGGAGGCTGCGCTGGAGTATTCGAGGAGTAGAGCTCATAATTTTATTGCACCGGAGCACATTGTCATCGGTTTATTGACTGTTGATGATGGGAGTGCTAGTCAGGTCCTTAAAAGACTGGCGGTAAATGTAAACCAGTTGTTAGCTGAGGCAGCCTCCAGACTTCAAATAGAGCTTGCCAAAGATGGTAGAGAGCCATCTGGGGGATCTCAAAAGACATTTTCTAAAAAGTCAACTGCATCGTCTGAGAAAACCAAAGAAAAAAGTGCTTTGGAGCGGTTCTGTGTGGATCTCACTGCTCGTGCTAGTGAGGGACGCATCGATCCAGTTATTGGCCGGGATACTGAAGTTCAGAGAATTATTCAGATACTTTGTCGCAGAACTAAAAATAATCCCATTCTTCTCGGTCAAAGTGGGGTTGGAAAGACAGCCATTGTTGAAGGTCTGGCAACTAGTATTGCACAGGCAGATGTCCCTGTTTATCTGTTGACAAAACGCGTAATGTCTTTGGACGTGGCACTGTTAATGGCTGGTGCAAAGGAGAGGGGAGAGTTGGAGGCGCGTGTTACCTCCTTAATAAGTGATGTACAAAAATCAGGGAATGTTATTCTGTTTATTGATGAAGTTCATACCCTTATTGAGTCCGGCACAGTTGGACGAGGAAGTAAGGGGTCTGGTCTTGACATTGCTAATATAATGAAACCGGCACTTGGGAGGGGTAAATTACAGTGTATTGCATCCACCACTACCGATGAATATAGGATGCATATTGAAAAGGATAAAGCATTTGGACGAAGATTCCAACCTGTCTGGATCAATGAACCAAGCCAGGATGATGCAGTTAGGATTCTGTTTGGTTTGCGTGAGAAATATGAGGCCCATCACAACTGCATATACGCACCAGAAGCCATTAGTGCTGCTGTGTACCTGTCCGCAAGATATATTCCTGATAGATATCTTCCTGATAAAGCTATCGATCTCCTTGATGAGGCGGGAAGTAGAGCTCGTATGGAAGCCTTTAAGAAGAAAAAAGAAGAGCGGGTTGGCATACTTTCAAAGTCACCTGATGATTATTGGCAAGAAATTCGTACAGTTCAGGCAATGCATGAAGTGGTCCTATCGAGTGAACTGAAAAATGGAGCTGCTTCTGTGGATGATACCAGAGAACATATTCTAGAGTCCATTTCATCTTCCACAGTAGATGATGAACCTACTGTGGTGGGACCAAATGATATAGCAGCAGTTGCCTCACTATGGTCAGGAGTCCCTCTTCAGCAGCTCACTGCTGATGACCGACTGCTTTTGGTGGATCTTGATGAGAAGCTCAGAAGACGGGTTGTTGGTCAAGATGAGGCTGTTGCAGCCATTTCTCGAGCTGTTAGGAGGTCCCGGGTTGGATTGAAGGATCCAAGTAGGCCCATGGCAACAATGCTCTTTTGCGGTCCTACTGGAGTTGGCAAAACTGAACTGACAAAAGCTTTGTCTGCATGTTATTTTGGATCGGTAAGTCTCCACTACTCTTTTTACATTGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009941 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022607 GO:0031967 GO:0031975 GO:0033554 GO:0034214 GO:0034605 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046983 GO:0047484 GO:0048518 GO:0048583 GO:0048584 GO:0050789 GO:0050896 GO:0051259 GO:0051716 GO:0065003 GO:0065007 GO:0071840 GO:0080134 GO:1901000 GO:1901002 GO:1902584 GO:2000070

KEGG Pathways

Metabolic & Signaling

ko01100 map01100

Pfam Domains

Protein Families

AAA AAA_2 ClpB_D2-small Clp_N

Protein Analysis

709

Amino Acids

77.97

Weight (kDa)

9.33

Isoelectric Point (pI)

52.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	190 - 260	6.4e-11	Clp repeat (R) N-terminal domain
AAA_16	PF13191	307 - 361	4e-06	AAA ATPase domain
NBD_SMAX1	PF23569	313 - 413	4.3e-06	SMAX1 nucleotide binding domain
AAA	PF00004	330 - 446	4e-09	ATPase family associated with various cellular activities (AAA)
AAA_lid_9	PF17871	473 - 550	1.1e-26	AAA lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013822)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51070
fragaria_vesca	FvH4_5g10020
malus_domestica	MD06G1128400.v1.1
prunus_persica	Prupe.5G138200_v2.0.a1
pyrus_communis	pycom06g11860
rosa_chinensis	RchiOBHm_Chr7g0188241
rosa_laevigata	RLG00000004715
rosa_multiflora	Rmu_co8357845.1_g000001 Rmu_co8388145.1_g000001 Rmu_sc0008750.1_g000013
rosa_roxburghii	Rroxscaffold_3G00266580
rosa_rugosa	Rorug06G0489400
rosa_samantha	Rh7AG093700 Rh7BG095100 Rh7CG095500 Rh7DG095700
rosa_wichuraiana	Rw7G008070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	923
AccBSI	CCGCTC	4 cut(s)	221, 286, 868, 1630
AccII	CGCG	7 cut(s)	300, 302, 433, 435, 477, 1089, 1153
AccIII	TCCGGA	1 cut(s)	483
AclWI	GGATC	9 cut(s)	567, 805, 888, 908, 1412, 1908, 1991, 2004, 2098
AcoI	YGGCCR	1 cut(s)	925
AcsI	RAATTY	2 cut(s)	427, 1674
AcuI	CTGAAG	2 cut(s)	957, 1847
AfaI	GTAC	4 cut(s)	93, 1179, 1517, 1681
AfiI	CCNNNNNNNGG	8 cut(s)	230, 355, 466, 794, 923, 1301, 1579, 1845
AflIII	ACRYGT	2 cut(s)	67, 1087
AgsI	TTSAA	6 cut(s)	758, 1031, 1364, 1647, 1993, 2123
AhlI	ACTAGT	1 cut(s)	1043
AjiI	CACGTC	1 cut(s)	1105
AjnI	CCWGG	2 cut(s)	459, 1419
AjuI	GAANNNNNNNTTGG	2 cut(s)	1617, 1649
Alw21I	GWGCWC	3 cut(s)	628, 651, 1597
Alw26I	GTCTC	2 cut(s)	274, 2105
AlwI	GGATC	9 cut(s)	567, 805, 888, 908, 1412, 1908, 1991, 2004, 2098
Ama87I	CYCGRG	3 cut(s)	443, 1962, 1980
Aor13HI	TCCGGA	1 cut(s)	483
AoxI	GGCC	5 cut(s)	346, 468, 925, 1467, 2006
ApoI	RAATTY	2 cut(s)	427, 1674
ArsI	GACNNNNNNTTYG	8 cut(s)	502, 508, 534, 540, 2053, 2057, 2085, 2089
Asp700I	GAANNNNTTC	1 cut(s)	1761
AspLEI	GCGC	6 cut(s)	300, 302, 435, 479, 604, 1153
AsuC2I	CCSGG	5 cut(s)	350, 546, 929, 1981, 1982
AsuHPI	GGTGA	1 cut(s)	1644
AvaI	CYCGRG	3 cut(s)	443, 1962, 1980
AvaII	GGWCC	6 cut(s)	542, 697, 1705, 1814, 1977, 2033
BaeI	ACNNNNGTAYC	2 cut(s)	924, 957
BamHI	GGATCC	1 cut(s)	1996
BanII	GRGCYC	2 cut(s)	628, 1597
BauI	CACGAG	1 cut(s)	891
BbsI	GAAGAC	2 cut(s)	271, 1927
Bbv12I	GWGCWC	3 cut(s)	628, 651, 1597
BbvCI	CCTCAGC	1 cut(s)	738
BccI	CCATC	6 cut(s)	67, 423, 674, 770, 796, 1479
BceAI	ACGGC	5 cut(s)	131, 247, 260, 296, 464
BcgI	CGANNNNNNTGC	4 cut(s)	392, 426, 1658, 1692
BciT130I	CCWGG	2 cut(s)	461, 1421
BciVI	GTATCC	1 cut(s)	925
BcnI	CCSGG	5 cut(s)	350, 546, 929, 1981, 1982
BcoDI	GTCTC	2 cut(s)	274, 2105
BcuI	ACTAGT	1 cut(s)	1043
BfaI	CTAG	5 cut(s)	555, 690, 897, 1044, 1766
BfuI	GTATCC	1 cut(s)	925
Bme1390I	CCNGG	7 cut(s)	350, 461, 546, 929, 1421, 1981, 1982
Bme18I	GGWCC	6 cut(s)	542, 697, 1705, 1814, 1977, 2033
BmeT110I	CYCGRG	3 cut(s)	443, 1962, 1980
BmgBI	CACGTC	1 cut(s)	1105
BmiI	GGNNCC	3 cut(s)	1815, 1979, 1998
BmrFI	CCNGG	7 cut(s)	350, 461, 546, 929, 1421, 1981, 1982
BmsI	GCATC	6 cut(s)	418, 839, 918, 1337, 1344, 1417
BpiI	GAAGAC	2 cut(s)	271, 1927
BplI	GAGNNNNNCTC	4 cut(s)	610, 642, 731, 763
BpmI	CTGGAG	5 cut(s)	482, 614, 626, 733, 2061
Bpu10I	CCTNAGC	1 cut(s)	738
BpuMI	CCSGG	5 cut(s)	350, 546, 929, 1981, 1982
Bsa29I	ATCGAT	2 cut(s)	912, 1566
BsaAI	YACGTR	1 cut(s)	70
BsaBI	GATNNNNATC	1 cut(s)	1545
BsaJI	CCNNGG	4 cut(s)	393, 459, 1980, 2010
BsaWI	WCCGGW	3 cut(s)	237, 483, 643
BsaXI	ACNNNNNCTCC	2 cut(s)	676, 706
Bsc4I	CCNNNNNNNGG	8 cut(s)	230, 355, 466, 794, 923, 1301, 1579, 1845
Bse118I	RCCGGY	1 cut(s)	1294
Bse1I	ACTGG	4 cut(s)	714, 727, 917, 2044
Bse3DI	GCAATG	2 cut(s)	1275, 1698
Bse8I	GATNNNNATC	1 cut(s)	1545
BseAI	TCCGGA	1 cut(s)	483
BseBI	CCWGG	2 cut(s)	461, 1421
BseCI	ATCGAT	2 cut(s)	912, 1566
BseDI	CCNNGG	4 cut(s)	393, 459, 1980, 2010
BseGI	GGATG	6 cut(s)	14, 421, 1328, 1359, 1429, 1750
BseJI	GATNNNNATC	1 cut(s)	1545
BseLI	CCNNNNNNNGG	8 cut(s)	230, 355, 466, 794, 923, 1301, 1579, 1845
BseMI	GCAATG	2 cut(s)	1275, 1698
BseMII	CTCAG	4 cut(s)	380, 729, 828, 1930
BseNI	ACTGG	4 cut(s)	714, 727, 917, 2044
BsePI	GCGCGC	1 cut(s)	298
BseRI	GAGGAG	5 cut(s)	10, 256, 410, 413, 631
Bsh1236I	CGCG	7 cut(s)	300, 302, 433, 435, 477, 1089, 1153
Bsh1285I	CGRYCG	1 cut(s)	238
BshFI	GGCC	5 cut(s)	348, 470, 927, 1469, 2008
BshVI	ATCGAT	2 cut(s)	912, 1566
BsiEI	CGRYCG	1 cut(s)	238
BsiHKAI	GWGCWC	3 cut(s)	628, 651, 1597
BsiHKCI	CYCGRG	3 cut(s)	443, 1962, 1980
BsiSI	CCGG	9 cut(s)	238, 349, 484, 545, 644, 928, 1236, 1295, 1981
BslFI	GGGAC	7 cut(s)	190, 365, 918, 1049, 1827, 1841, 1963
BslI	CCNNNNNNNGG	8 cut(s)	230, 355, 466, 794, 923, 1301, 1579, 1845
BsmAI	GTCTC	2 cut(s)	274, 2105
BsmBI	CGTCTC	1 cut(s)	274
BsmFI	GGGAC	7 cut(s)	190, 365, 918, 1049, 1827, 1841, 1963
BsnI	GGCC	5 cut(s)	348, 470, 927, 1469, 2008
BsoBI	CYCGRG	3 cut(s)	443, 1962, 1980
Bsp1286I	GDGCHC	3 cut(s)	628, 651, 1597
Bsp13I	TCCGGA	1 cut(s)	483
Bsp1407I	TGTACA	1 cut(s)	1177
Bsp19I	CCATGG	1 cut(s)	2010
BspANI	GGCC	5 cut(s)	348, 470, 927, 1469, 2008
BspCNI	CTCAG	4 cut(s)	379, 730, 829, 1929
BspDI	ATCGAT	2 cut(s)	912, 1566
BspEI	TCCGGA	1 cut(s)	483
BspFNI	CGCG	7 cut(s)	300, 302, 433, 435, 477, 1089, 1153
BspLI	GGNNCC	3 cut(s)	1815, 1979, 1998
BspPI	GGATC	9 cut(s)	567, 805, 888, 908, 1412, 1908, 1991, 2004, 2098
BspQI	GCTCTTC	1 cut(s)	1620
BsrBI	CCGCTC	4 cut(s)	221, 286, 868, 1630
BsrDI	GCAATG	2 cut(s)	1275, 1698
BsrFI	RCCGGY	1 cut(s)	1294
BsrGI	TGTACA	1 cut(s)	1177
BsrI	ACTGG	4 cut(s)	714, 727, 917, 2044
BssAI	RCCGGY	1 cut(s)	1294
BssECI	CCNNGG	4 cut(s)	393, 459, 1980, 2010
BssHII	GCGCGC	1 cut(s)	298
BssSI	CACGAG	1 cut(s)	891
BssT1I	CCWWGG	1 cut(s)	2010
Bst2BI	CACGAG	1 cut(s)	891
Bst2UI	CCWGG	2 cut(s)	461, 1421
Bst4CI	ACNGT	7 cut(s)	673, 1113, 1243, 1320, 1684, 1789, 1807
Bst6I	CTCTTC	2 cut(s)	1620, 1866
BstAPI	GCANNNNNTGC	2 cut(s)	376, 1835
BstAUI	TGTACA	1 cut(s)	1177
BstBAI	YACGTR	1 cut(s)	70
BstC8I	GCNNGC	5 cut(s)	300, 468, 472, 535, 768
BstDEI	CTNAG	4 cut(s)	366, 738, 837, 1916
BstDSI	CCRYGG	1 cut(s)	2010
BstENI	CCTNNNNNAGG	2 cut(s)	464, 1577
BstF5I	GGATG	6 cut(s)	14, 421, 1328, 1359, 1429, 1750
BstFNI	CGCG	7 cut(s)	300, 302, 433, 435, 477, 1089, 1153
BstHHI	GCGC	6 cut(s)	300, 302, 435, 479, 604, 1153
BstMAI	GTCTC	2 cut(s)	274, 2105
BstMCI	CGRYCG	1 cut(s)	238
BstMWI	GCNNNNNNNGC	9 cut(s)	308, 376, 476, 743, 1496, 1505, 1835, 1952, 2075
BstNI	CCWGG	2 cut(s)	461, 1421
BstNSI	RCATGY	2 cut(s)	8, 2082
BstSCI	CCNGG	7 cut(s)	348, 459, 544, 927, 1419, 1979, 1980
BstUI	CGCG	7 cut(s)	300, 302, 433, 435, 477, 1089, 1153
BstV2I	GAAGAC	2 cut(s)	271, 1927
BstX2I	RGATCY	5 cut(s)	559, 797, 880, 1900, 1996
BstXI	CCANNNNNNTGG	2 cut(s)	48, 343
BstYI	RGATCY	5 cut(s)	559, 797, 880, 1900, 1996
Bsu15I	ATCGAT	2 cut(s)	912, 1566
BsuI	GTATCC	1 cut(s)	925
BsuRI	GGCC	5 cut(s)	348, 470, 927, 1469, 2008
BsuTUI	ATCGAT	2 cut(s)	912, 1566
BtgI	CCRYGG	1 cut(s)	2010
BtgZI	GCGATG	1 cut(s)	395
BtrI	CACGTC	1 cut(s)	1105
BtsCI	GGATG	6 cut(s)	14, 421, 1328, 1359, 1429, 1750
BtsI	GCAGTG	2 cut(s)	885, 1872
BtsIMutI	CAGTG	4 cut(s)	885, 1109, 1325, 1872
Cac8I	GCNNGC	5 cut(s)	300, 468, 472, 535, 768
CfoI	GCGC	6 cut(s)	300, 302, 435, 479, 604, 1153
Cfr10I	RCCGGY	1 cut(s)	1294
Cfr9I	CCCGGG	1 cut(s)	1980
ClaI	ATCGAT	2 cut(s)	912, 1566
CseI	GACGC	1 cut(s)	915
Csp6I	GTAC	4 cut(s)	92, 1178, 1516, 1680
CviAII	CATG	6 cut(s)	5, 355, 454, 1697, 2011, 2079
CviQI	GTAC	4 cut(s)	92, 1178, 1516, 1680
DdeI	CTNAG	4 cut(s)	366, 738, 837, 1916
EaeI	YGGCCR	1 cut(s)	925
Eam1104I	CTCTTC	2 cut(s)	1620, 1866
EarI	CTCTTC	2 cut(s)	1620, 1866
EciI	GGCGGA	1 cut(s)	262
Ecl136II	GAGCTC	2 cut(s)	626, 1595
Eco130I	CCWWGG	1 cut(s)	2010
Eco24I	GRGCYC	2 cut(s)	628, 1597
Eco32I	GATATC	2 cut(s)	33, 1547
Eco47I	GGWCC	6 cut(s)	542, 697, 1705, 1814, 1977, 2033
Eco53kI	GAGCTC	2 cut(s)	626, 1595
Eco57I	CTGAAG	2 cut(s)	957, 1847
Eco88I	CYCGRG	3 cut(s)	443, 1962, 1980
EcoICRI	GAGCTC	2 cut(s)	626, 1595
EcoNI	CCTNNNNNAGG	2 cut(s)	464, 1577
EcoO109I	RGGNCCY	2 cut(s)	697, 1977
EcoRI	GAATTC	1 cut(s)	427
EcoRII	CCWGG	2 cut(s)	459, 1419
EcoRV	GATATC	2 cut(s)	33, 1547
EcoT14I	CCWWGG	1 cut(s)	2010
EcoT22I	ATGCAT	3 cut(s)	6, 1359, 1698
EcoT38I	GRGCYC	2 cut(s)	628, 1597
ErhI	CCWWGG	1 cut(s)	2010
Esp3I	CGTCTC	1 cut(s)	274
FaeI	CATG	6 cut(s)	8, 358, 457, 1700, 2014, 2082
FaqI	GGGAC	7 cut(s)	190, 365, 918, 1049, 1827, 1841, 1963
FatI	CATG	6 cut(s)	4, 354, 453, 1696, 2010, 2078
FauI	CCCGC	4 cut(s)	428, 479, 1576, 1623
FokI	GGATG	5 cut(s)	408, 1315, 1366, 1436, 1757
FriOI	GRGCYC	2 cut(s)	628, 1597
FspBI	CTAG	5 cut(s)	555, 690, 897, 1044, 1766
GlaI	GCGC	6 cut(s)	299, 301, 434, 478, 603, 1152
GsuI	CTGGAG	5 cut(s)	482, 614, 626, 733, 2061
HaeIII	GGCC	5 cut(s)	348, 470, 927, 1469, 2008
HapII	CCGG	9 cut(s)	238, 349, 484, 545, 644, 928, 1236, 1295, 1981
HgaI	GACGC	1 cut(s)	915
HhaI	GCGC	6 cut(s)	300, 302, 435, 479, 604, 1153
Hin1II	CATG	6 cut(s)	8, 358, 457, 1700, 2014, 2082
Hin6I	GCGC	6 cut(s)	298, 300, 433, 477, 602, 1151
HinP1I	GCGC	6 cut(s)	298, 300, 433, 477, 602, 1151
HincII	GTYRAC	2 cut(s)	825, 1080
HindII	GTYRAC	2 cut(s)	825, 1080
HindIII	AAGCTT	1 cut(s)	2067
HinfI	GANTC	5 cut(s)	1232, 1389, 1438, 1769, 1854
HpaII	CCGG	9 cut(s)	238, 349, 484, 545, 644, 928, 1236, 1295, 1981
HphI	GGTGA	1 cut(s)	1644
Hpy166II	GTNNAC	7 cut(s)	291, 363, 724, 825, 1080, 1516, 1718
Hpy188I	TCNGA	5 cut(s)	426, 838, 945, 957, 1919
Hpy8I	GTNNAC	7 cut(s)	291, 363, 724, 825, 1080, 1516, 1718
Hpy99I	CGWCG	1 cut(s)	152
HpyAV	CCTTC	3 cut(s)	208, 1025, 1987
HpyCH4III	ACNGT	7 cut(s)	673, 1113, 1243, 1320, 1684, 1789, 1807
HpyCH4IV	ACGT	2 cut(s)	69, 1104
HpyF10VI	GCNNNNNNNGC	9 cut(s)	308, 376, 476, 743, 1496, 1505, 1835, 1952, 2075
HpyF3I	CTNAG	4 cut(s)	366, 738, 837, 1916
HpySE526I	ACGT	2 cut(s)	69, 1104
Hsp92II	CATG	6 cut(s)	8, 358, 457, 1700, 2014, 2082
HspAI	GCGC	6 cut(s)	298, 300, 433, 477, 602, 1151
Kpn2I	TCCGGA	1 cut(s)	483
LguI	GCTCTTC	1 cut(s)	1620
LmnI	GCTCC	9 cut(s)	226, 264, 295, 400, 463, 486, 646, 865, 1730
LweI	GCATC	6 cut(s)	418, 839, 918, 1337, 1344, 1417
MaeI	CTAG	5 cut(s)	555, 690, 897, 1044, 1766
MaeII	ACGT	2 cut(s)	69, 1104
MaeIII	GTNAC	4 cut(s)	100, 122, 1156, 1650
MbiI	CCGCTC	4 cut(s)	221, 286, 868, 1630
MflI	RGATCY	5 cut(s)	559, 797, 880, 1900, 1996
MhlI	GDGCHC	3 cut(s)	628, 651, 1597
MluCI	AATT	6 cut(s)	165, 427, 631, 948, 1313, 1674
MluI	ACGCGT	1 cut(s)	1087
MlyI	GAGTC	3 cut(s)	1241, 1778, 1863
MmeI	TCCRAC	5 cut(s)	991, 1125, 1225, 1417, 1966
Mph1103I	ATGCAT	3 cut(s)	6, 1359, 1698
MroI	TCCGGA	1 cut(s)	483
MroXI	GAANNNNTTC	1 cut(s)	1761
MseI	TTAA	4 cut(s)	702, 1115, 1166, 1611
MslI	CAYNNNNRTG	1 cut(s)	46
MspI	CCGG	9 cut(s)	238, 349, 484, 545, 644, 928, 1236, 1295, 1981
MspR9I	CCNGG	7 cut(s)	350, 461, 546, 929, 1421, 1981, 1982
MvaI	CCWGG	2 cut(s)	461, 1421
MvnI	CGCG	7 cut(s)	300, 302, 433, 435, 477, 1089, 1153
MwoI	GCNNNNNNNGC	9 cut(s)	308, 376, 476, 743, 1496, 1505, 1835, 1952, 2075
NciI	CCSGG	5 cut(s)	350, 546, 929, 1981, 1982
NcoI	CCATGG	1 cut(s)	2010
NlaIII	CATG	6 cut(s)	8, 358, 457, 1700, 2014, 2082
NlaIV	GGNNCC	3 cut(s)	1815, 1979, 1998
NmeAIII	GCCGAG	1 cut(s)	418
NmuCI	GTSAC	1 cut(s)	1650
NsiI	ATGCAT	3 cut(s)	6, 1359, 1698
NspI	RCATGY	2 cut(s)	8, 2082
PaeR7I	CTCGAG	1 cut(s)	1962
PauI	GCGCGC	1 cut(s)	298
PciSI	GCTCTTC	1 cut(s)	1620
PcsI	WCGNNNNNNNCGW	2 cut(s)	273, 299
PdmI	GAANNNNTTC	1 cut(s)	1761
PfeI	GAWTC	2 cut(s)	1389, 1438
PflMI	CCANNNNNTGG	1 cut(s)	923
PleI	GAGTC	3 cut(s)	1240, 1777, 1862
PpsI	GAGTC	3 cut(s)	1240, 1777, 1862
Ppu21I	YACGTR	1 cut(s)	70
PpuMI	RGGWCCY	2 cut(s)	697, 1977
Psp124BI	GAGCTC	2 cut(s)	628, 1597
Psp5II	RGGWCCY	2 cut(s)	697, 1977
Psp6I	CCWGG	2 cut(s)	459, 1419
PspGI	CCWGG	2 cut(s)	459, 1419
PspN4I	GGNNCC	3 cut(s)	1815, 1979, 1998
PspPPI	RGGWCCY	2 cut(s)	697, 1977
PsuI	RGATCY	5 cut(s)	559, 797, 880, 1900, 1996
PteI	GCGCGC	1 cut(s)	298
RsaI	GTAC	4 cut(s)	93, 1179, 1517, 1681
RsaNI	GTAC	4 cut(s)	92, 1178, 1516, 1680
RseI	CAYNNNNRTG	1 cut(s)	46
SacI	GAGCTC	2 cut(s)	628, 1597
SapI	GCTCTTC	1 cut(s)	1620
SaqAI	TTAA	4 cut(s)	702, 1115, 1166, 1611
SchI	GAGTC	3 cut(s)	1241, 1778, 1863
ScrFI	CCNGG	7 cut(s)	350, 461, 546, 929, 1421, 1981, 1982
SduI	GDGCHC	3 cut(s)	628, 651, 1597
SfaNI	GCATC	6 cut(s)	418, 839, 918, 1337, 1344, 1417
Sfr274I	CTCGAG	1 cut(s)	1962
SinI	GGWCC	6 cut(s)	542, 697, 1705, 1814, 1977, 2033
SlaI	CTCGAG	1 cut(s)	1962
SmaI	CCCGGG	1 cut(s)	1982
SmiMI	CAYNNNNRTG	1 cut(s)	46
SmlI	CTYRAG	1 cut(s)	1962
SmoI	CTYRAG	1 cut(s)	1962
SpeI	ACTAGT	1 cut(s)	1043
Sse9I	AATT	6 cut(s)	165, 427, 631, 948, 1313, 1674
SspMI	CTAG	5 cut(s)	555, 690, 897, 1044, 1766
SstI	GAGCTC	2 cut(s)	628, 1597
StyD4I	CCNGG	7 cut(s)	348, 459, 544, 927, 1419, 1979, 1980
StyI	CCWWGG	1 cut(s)	2010
TaaI	ACNGT	7 cut(s)	673, 1113, 1243, 1320, 1684, 1789, 1807
TaiI	ACGT	2 cut(s)	72, 1107
TaqI	TCGA	8 cut(s)	254, 282, 572, 614, 912, 1566, 1712, 1963
TaqII	GACCGA	2 cut(s)	986, 1899
TasI	AATT	6 cut(s)	165, 427, 631, 948, 1313, 1674
TatI	WGTACW	1 cut(s)	1177
TauI	GCSGC	3 cut(s)	221, 477, 499
TfiI	GAWTC	2 cut(s)	1389, 1438
Tru1I	TTAA	4 cut(s)	702, 1115, 1166, 1611
Tru9I	TTAA	4 cut(s)	702, 1115, 1166, 1611
TscAI	CASTG	4 cut(s)	892, 1116, 1325, 1879
TseFI	GTSAC	1 cut(s)	1650
Tsp45I	GTSAC	1 cut(s)	1650
TspDTI	ATGAA	9 cut(s)	410, 1208, 1227, 1304, 1359, 1425, 1713, 1767, 1812
TspGWI	ACGGA	3 cut(s)	13, 88, 308
TspMI	CCCGGG	1 cut(s)	1980
TspRI	CASTG	4 cut(s)	892, 1116, 1325, 1879
Van91I	CCANNNNNTGG	1 cut(s)	923
VpaK11BI	GGWCC	6 cut(s)	542, 697, 1705, 1814, 1977, 2033
XagI	CCTNNNNNAGG	2 cut(s)	464, 1577
XapI	RAATTY	2 cut(s)	427, 1674
XbaI	TCTAGA	1 cut(s)	1765
XceI	RCATGY	2 cut(s)	8, 2082
XhoI	CTCGAG	1 cut(s)	1962
XmaI	CCCGGG	1 cut(s)	1980
XmnI	GAANNNNTTC	1 cut(s)	1761
XspI	CTAG	5 cut(s)	555, 690, 897, 1044, 1766
Zsp2I	ATGCAT	3 cut(s)	6, 1359, 1698

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.