Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0002495

Physical Location & Seq

Genomic Coordinates Reverse (-)

6381268 .. 6384515

Gene Structure 3248 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g11251.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1863 bp

ATGGATTTCTGTGCTGGTTTGATTGGTGTGAAGTCTGACTGTGAATGTGAATTTGTGGAGGAAGATCCGACTGGGAGATTCTTAAGGTCTGATGAAATCTTGGGGAGGGGAGCATTCAAGACTGTATATAAGGCGTTTGATGAGGTTGAAGGTTTAGAGGTGGCATGGAGCCAGGTGCATGTTGAGGATGTGTTGCAGACGCCGGAGCAACTTCAGAGGTTGTATTCTGAGGTTCATTTGCTGAAGTTGTTGAAGCATGAAAATATCATCAAGTTCTGTAACTGGTGGGTTGATGATAAGAACAAGACTATCAACATTATCACGGAGTTGTTCACTTCTGGGAGCTTGAGGCAGTACCGAAGGAAGCATAAGAGTGTTGAATTGAAGGCCATCAAGAAGTGGGCAAGGCAGATTCTTCGAGGCTTGCACTATCTGCATTCTCATGATCCTCCAATTATTCATAGAGATTTGAAATGCGACAATATATTTGTCAATGGAAATCAAGGGGAAATAAAAATTGGAGATCTTGGATTGGCAATAGTCATGCAGCAACCTACTGCCCATAGTGTGATCGGGACTCCAGAATTCATGGCTCCCGAGCTTTACGACGAGGAATATAATGAATTAGTCGACATCTATTCTTTTGGCATGTGCATGCTAGAAATGGTGACTTGTGAATACCCTTACAATGAATGTAAAAATCCTGCACAGATTTATAAGAAGGTTACCAATGGTATAAAGCCTGCTTCACTCAGTAAAGTGAGTGATCCTCAAGTCAAGCAGTTCATAGAGAAGTGTCTGGTTCCAGCTTCAATGAGACTGCCTGCGGTGGAGCTCTTGAAAGATCCATTCCTTGCAATTGACAATATGAAGGAGCGTACCTGTTATCCATTGGGATCACACAATTTCATTCCCAAATTAGAGAACATGACACAGCCTGAAGCTCGTCCAATGGATGTAGATGCTAACTGCCAGAAGATTTCTGCTGGCTCTTGCAGGAAAAGCGTCGATGTAACTTCCATCTCATCCGACCCCTCAGTTCTTGAATTACAAAGATTCACAGAGAGTAATGAATTCACTTTAAGAGCAGAGAAAAGTGCTGATAATACCATCGCATTGACTTTGCGTATTGTTGATCCATGTGGTCATGGTAAGACTATCCATTTTGAGTTCTACCTTCATACAGATACTGCAATTTCAATTGCTGGGGAGATGGCTGAGCAACTTGATCTCTCAACTGAAGATGTATCCGTCACAGCTGAGTTAATTGATAGCTTGATTATGAAGCTTGTACCCAGCTGGAAACCTACATCTGACAGTTCATCATGTGGAGCAAATAGTTCATATGGGGATCAGTATTTATGTCAAGTTGATGGAAGTCATTGGGATTCAGGGTTTATCAATCCTACATCAATGATTTCAGATATCTCAGCTGAGTACGGCGTCACTAATGTCTCAGGCACCACTGATCTCAAGGTTTTAGAATCAGACATGTATAGTTTTGATGAATGCTATAGAGGTTCAGATGGGTACTGTTCGAGTCCAGATTGTTTGGTTCATGGTCAGCTTAAGCAGAAGAAATATGAAGATGATTTTGGTGATTCTATCGTGATAAACGACTCAGCAATGAACTCTTCTATCTCGAGCCCTGATAGATCAAAAACTTGTAGCTTATCAAGCATTTGTTCCTTGTCAGAATTGTCTTTGATGGAGAAACATGCGTATGATGGGCTCAAAGTGGAGCTGGATTCAATTGATCTGCAGTTTCATCAACATTTTCTTGAGCTCTTGAAGATGAAGGAAGAGGCAATACATAACGCAAAGAAGAAGTGGATAACAAAGAAGAGAATAGCTGTTCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0035556 GO:0036211 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0048518 GO:0048573 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051716 GO:0061458 GO:0065007 GO:0071704 GO:0140096 GO:1901564 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

OSR1_C Pkinase

Protein Analysis

621

Amino Acids

69.87

Weight (kDa)

5.21

Isoelectric Point (pI)

35.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	5 - 104	5.9e-11	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	13 - 88	6.4e-08	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002495)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64630 AT5G41990
fragaria_vesca	FvH4_5g11251 FvH4_5g11251 FvH4_6g41800 FvH4_6g41920 FvH4_6g42720
malus_domestica	MD06G1114800.v1.1 MD06G1115000.v1.1 MD13G1200200.v1.1 MD14G1136200.v1.1
prunus_persica	Prupe.5G127500_v2.0.a1 Prupe.5G127500_v2.0.a1
pyrus_communis	pycom06g10760 pycom13g17380 pycom13g17390 pycom14g10980
rosa_chinensis	RchiOBHm_Chr2g0157811 RchiOBHm_Chr7g0186611
rosa_laevigata	RLG00000004829 RLG00000021022
rosa_multiflora	Rmu_co8298477.1_g000001 Rmu_sc0000645.1_g000017 Rmu_sc0007722.1_g000019
rosa_roxburghii	Rroxscaffold_2G00091930 Rroxscaffold_3G00268010
rosa_rugosa	Rorug02G0463900 Rorug06G0475200 Rorug06G0475300 Rorug06G0475400
rosa_samantha	Rh2AG529200 Rh2BG543300 Rh2CG513500 Rh2DG552100 Rh7AG065500 Rh7BG081400 Rh7CG082300 Rh7DG083300
rosa_wichuraiana	Rw2G043750 Rw7G006920 Rw7G007140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	717
AccB1I	GGYRCC	1 cut(s)	1460
AccI	GTMKAC	1 cut(s)	630
AciI	CCGC	1 cut(s)	827
AclWI	GGATC	7 cut(s)	59, 440, 761, 839, 904, 1130, 1359
AcsI	RAATTY	3 cut(s)	50, 584, 1073
AcuI	CTGAAG	4 cut(s)	197, 263, 960, 1260
AcyI	GRCGYC	2 cut(s)	200, 1443
AfaI	GTAC	5 cut(s)	356, 880, 1293, 1439, 1532
AflII	CTTAAG	2 cut(s)	82, 1568
AflIII	ACRYGT	1 cut(s)	1491
AjnI	CCWGG	1 cut(s)	171
AjuI	GAANNNNNNNTTGG	4 cut(s)	501, 533, 1578, 1610
Alw21I	GWGCWC	2 cut(s)	837, 1788
Alw26I	GTCTC	2 cut(s)	811, 1459
AlwI	GGATC	7 cut(s)	59, 440, 761, 839, 904, 1130, 1359
AlwNI	CAGNNNCTG	1 cut(s)	1190
Ama87I	CYCGRG	2 cut(s)	596, 1642
AoxI	GGCC	1 cut(s)	387
ApeKI	GCWGC	1 cut(s)	547
ApoI	RAATTY	3 cut(s)	50, 584, 1073
ArsI	GACNNNNNNTTYG	2 cut(s)	470, 502
AsuHPI	GGTGA	2 cut(s)	679, 1610
AvaI	CYCGRG	2 cut(s)	596, 1642
BanI	GGYRCC	1 cut(s)	1460
BanII	GRGCYC	4 cut(s)	837, 1649, 1734, 1788
BarI	GAAGNNNNNNTAC	2 cut(s)	1794, 1826
Bbv12I	GWGCWC	2 cut(s)	837, 1788
BbvI	GCAGC	1 cut(s)	559
BccI	CCATC	8 cut(s)	398, 1028, 1118, 1207, 1367, 1520, 1702, 1721
BceAI	ACGGC	1 cut(s)	1456
BcgI	CGANNNNNNTGC	2 cut(s)	398, 432
BciT130I	CCWGG	1 cut(s)	173
BciVI	GTATCC	1 cut(s)	1258
BcoDI	GTCTC	2 cut(s)	811, 1459
BfaI	CTAG	1 cut(s)	659
BfmI	CTRYAG	2 cut(s)	1513, 1760
BfrI	CTTAAG	2 cut(s)	82, 1568
BfuI	GTATCC	1 cut(s)	1258
BglII	AGATCT	1 cut(s)	523
BisI	GCNGC	1 cut(s)	548
BlpI	GCTNAGC	1 cut(s)	1218
BlsI	GCNGC	1 cut(s)	549
Bme1390I	CCNGG	1 cut(s)	173
BmeT110I	CYCGRG	2 cut(s)	596, 1642
BmiI	GGNNCC	4 cut(s)	170, 594, 804, 1462
BmrFI	CCNGG	1 cut(s)	173
BmrI	ACTGGG	1 cut(s)	81
BmsI	GCATC	1 cut(s)	952
BmuI	ACTGGG	1 cut(s)	81
BplI	GAGNNNNNCTC	2 cut(s)	754, 786
BpmI	CTGGAG	1 cut(s)	564
Bpu1102I	GCTNAGC	1 cut(s)	1218
BpuEI	CTTGAG	4 cut(s)	367, 756, 1457, 1802
BsaHI	GRCGYC	2 cut(s)	200, 1443
Bse1I	ACTGG	2 cut(s)	76, 287
Bse3DI	GCAATG	1 cut(s)	1632
BseBI	CCWGG	1 cut(s)	173
BseGI	GGATG	3 cut(s)	193, 961, 1025
BseMI	GCAATG	1 cut(s)	1632
BseMII	CTCAG	9 cut(s)	219, 766, 1050, 1209, 1251, 1425, 1443, 1470, 1635
BseNI	ACTGG	2 cut(s)	76, 287
BseXI	GCAGC	1 cut(s)	559
BseYI	CCCAGC	2 cut(s)	1205, 1295
BsgI	GTGCAG	1 cut(s)	690
BshFI	GGCC	1 cut(s)	389
BshNI	GGYRCC	1 cut(s)	1460
BsiHKAI	GWGCWC	2 cut(s)	837, 1788
BsiHKCI	CYCGRG	2 cut(s)	596, 1642
BsiSI	CCGG	1 cut(s)	203
BslFI	GGGAC	1 cut(s)	589
BsmAI	GTCTC	2 cut(s)	811, 1459
BsmFI	GGGAC	1 cut(s)	589
BsmI	GAATGC	3 cut(s)	113, 436, 1514
BsnI	GGCC	1 cut(s)	389
BsoBI	CYCGRG	2 cut(s)	596, 1642
Bsp1286I	GDGCHC	4 cut(s)	837, 1649, 1734, 1788
Bsp1720I	GCTNAGC	1 cut(s)	1218
BspACI	CCGC	1 cut(s)	827
BspANI	GGCC	1 cut(s)	389
BspCNI	CTCAG	9 cut(s)	220, 765, 1049, 1210, 1252, 1426, 1442, 1469, 1634
BspHI	TCATGA	1 cut(s)	442
BspLI	GGNNCC	4 cut(s)	170, 594, 804, 1462
BspMAI	CTGCAG	1 cut(s)	1764
BspPI	GGATC	7 cut(s)	59, 440, 761, 839, 904, 1130, 1359
BspT107I	GGYRCC	1 cut(s)	1460
BspTI	CTTAAG	2 cut(s)	82, 1568
BsrDI	GCAATG	1 cut(s)	1632
BsrI	ACTGG	2 cut(s)	76, 287
BssNI	GRCGYC	2 cut(s)	200, 1443
Bst2UI	CCWGG	1 cut(s)	173
Bst4CI	ACNGT	4 cut(s)	41, 124, 1319, 1535
Bst6I	CTCTTC	3 cut(s)	1639, 1797, 1838
BstACI	GRCGYC	2 cut(s)	200, 1443
BstAFI	CTTAAG	2 cut(s)	82, 1568
BstAPI	GCANNNNNTGC	1 cut(s)	433
BstC8I	GCNNGC	5 cut(s)	425, 656, 744, 825, 988
BstDEI	CTNAG	9 cut(s)	228, 752, 1036, 1218, 1260, 1429, 1434, 1456, 1621
BstEII	GGTNACC	1 cut(s)	724
BstF5I	GGATG	3 cut(s)	193, 961, 1025
BstMAI	GTCTC	2 cut(s)	811, 1459
BstMWI	GCNNNNNNNGC	3 cut(s)	433, 1002, 1677
BstNI	CCWGG	1 cut(s)	173
BstNSI	RCATGY	5 cut(s)	182, 652, 658, 1495, 1721
BstPI	GGTNACC	1 cut(s)	724
BstSCI	CCNGG	1 cut(s)	171
BstSFI	CTRYAG	2 cut(s)	1513, 1760
BstV1I	GCAGC	1 cut(s)	559
BstX2I	RGATCY	3 cut(s)	64, 523, 844
BstYI	RGATCY	3 cut(s)	64, 523, 844
BsuI	GTATCC	1 cut(s)	1258
BsuRI	GGCC	1 cut(s)	389
BtgZI	GCGATG	1 cut(s)	1096
BtsCI	GGATG	3 cut(s)	193, 961, 1025
BtsIMutI	CAGTG	1 cut(s)	1464
Cac8I	GCNNGC	5 cut(s)	425, 656, 744, 825, 988
CaiI	CAGNNNCTG	1 cut(s)	1190
CciI	TCATGA	1 cut(s)	442
CseI	GACGC	3 cut(s)	208, 994, 1432
Csp6I	GTAC	5 cut(s)	355, 879, 1292, 1438, 1531
CviQI	GTAC	5 cut(s)	355, 879, 1292, 1438, 1531
DdeI	CTNAG	9 cut(s)	228, 752, 1036, 1218, 1260, 1429, 1434, 1456, 1621
Eam1104I	CTCTTC	3 cut(s)	1639, 1797, 1838
EarI	CTCTTC	3 cut(s)	1639, 1797, 1838
Ecl136II	GAGCTC	2 cut(s)	835, 1786
Eco24I	GRGCYC	4 cut(s)	837, 1649, 1734, 1788
Eco32I	GATATC	1 cut(s)	1426
Eco53kI	GAGCTC	2 cut(s)	835, 1786
Eco57I	CTGAAG	4 cut(s)	197, 263, 960, 1260
Eco88I	CYCGRG	2 cut(s)	596, 1642
Eco91I	GGTNACC	1 cut(s)	724
EcoICRI	GAGCTC	2 cut(s)	835, 1786
EcoO65I	GGTNACC	1 cut(s)	724
EcoRI	GAATTC	2 cut(s)	584, 1073
EcoRII	CCWGG	1 cut(s)	171
EcoRV	GATATC	1 cut(s)	1426
EcoT38I	GRGCYC	4 cut(s)	837, 1649, 1734, 1788
FaqI	GGGAC	1 cut(s)	589
FauNDI	CATATG	1 cut(s)	1345
FblI	GTMKAC	1 cut(s)	630
Fnu4HI	GCNGC	1 cut(s)	548
FokI	GGATG	3 cut(s)	200, 968, 1012
FriOI	GRGCYC	4 cut(s)	837, 1649, 1734, 1788
Fsp4HI	GCNGC	1 cut(s)	548
FspBI	CTAG	1 cut(s)	659
GluI	GCNGC	1 cut(s)	548
GsaI	CCCAGC	2 cut(s)	1209, 1299
GsuI	CTGGAG	1 cut(s)	564
HaeIII	GGCC	1 cut(s)	389
HapII	CCGG	1 cut(s)	203
HgaI	GACGC	3 cut(s)	208, 994, 1432
Hin1I	GRCGYC	2 cut(s)	200, 1443
HincII	GTYRAC	1 cut(s)	631
HindII	GTYRAC	1 cut(s)	631
HindIII	AAGCTT	1 cut(s)	1286
HpaII	CCGG	1 cut(s)	203
HphI	GGTGA	2 cut(s)	679, 1610
Hpy166II	GTNNAC	2 cut(s)	333, 631
Hpy8I	GTNNAC	2 cut(s)	333, 631
Hpy99I	CGWCG	2 cut(s)	611, 1010
HpyAV	CCTTC	7 cut(s)	143, 354, 379, 715, 865, 1187, 1792
HpyCH4III	ACNGT	4 cut(s)	41, 124, 1319, 1535
HpyF10VI	GCNNNNNNNGC	3 cut(s)	433, 1002, 1677
HpyF3I	CTNAG	9 cut(s)	228, 752, 1036, 1218, 1260, 1429, 1434, 1456, 1621
Hsp92I	GRCGYC	2 cut(s)	200, 1443
LmnI	GCTCC	9 cut(s)	110, 168, 205, 342, 598, 832, 874, 1331, 1741
Lsp1109I	GCAGC	1 cut(s)	559
LweI	GCATC	1 cut(s)	952
MaeI	CTAG	1 cut(s)	659
MaeIII	GTNAC	6 cut(s)	278, 667, 724, 1012, 1252, 1444
MfeI	CAATTG	3 cut(s)	858, 1200, 1752
MflI	RGATCY	3 cut(s)	64, 523, 844
MhlI	GDGCHC	4 cut(s)	837, 1649, 1734, 1788
MlyI	GAGTC	3 cut(s)	571, 1549, 1613
MmeI	TCCRAC	2 cut(s)	92, 1053
MseI	TTAA	5 cut(s)	83, 1082, 1265, 1569, 1861
MslI	CAYNNNNRTG	4 cut(s)	372, 441, 653, 1722
MspA1I	CMGCKG	3 cut(s)	1259, 1299, 1433
MspCI	CTTAAG	2 cut(s)	82, 1568
MspI	CCGG	1 cut(s)	203
MspR9I	CCNGG	1 cut(s)	173
MunI	CAATTG	3 cut(s)	858, 1200, 1752
Mva1269I	GAATGC	3 cut(s)	113, 436, 1514
MvaI	CCWGG	1 cut(s)	173
MwoI	GCNNNNNNNGC	3 cut(s)	433, 1002, 1677
NdeI	CATATG	1 cut(s)	1345
NlaIV	GGNNCC	4 cut(s)	170, 594, 804, 1462
NmuCI	GTSAC	3 cut(s)	667, 1252, 1444
NspI	RCATGY	5 cut(s)	182, 652, 658, 1495, 1721
PaeI	GCATGC	1 cut(s)	658
PaeR7I	CTCGAG	1 cut(s)	1642
PagI	TCATGA	1 cut(s)	442
PciI	ACATGT	1 cut(s)	1491
PcsI	WCGNNNNNNNCGW	1 cut(s)	1614
PctI	GAATGC	3 cut(s)	113, 436, 1514
PfeI	GAWTC	7 cut(s)	78, 412, 1056, 1388, 1484, 1601, 1748
PkrI	GCNGC	1 cut(s)	549
PleI	GAGTC	3 cut(s)	571, 1548, 1613
PpsI	GAGTC	3 cut(s)	571, 1548, 1613
PscI	ACATGT	1 cut(s)	1491
PsiI	TTATAA	1 cut(s)	717
Psp124BI	GAGCTC	2 cut(s)	837, 1788
Psp6I	CCWGG	1 cut(s)	171
PspEI	GGTNACC	1 cut(s)	724
PspFI	CCCAGC	2 cut(s)	1205, 1295
PspGI	CCWGG	1 cut(s)	171
PspN4I	GGNNCC	4 cut(s)	170, 594, 804, 1462
PstI	CTGCAG	1 cut(s)	1764
PstNI	CAGNNNCTG	1 cut(s)	1190
PsuI	RGATCY	3 cut(s)	64, 523, 844
PvuII	CAGCTG	3 cut(s)	1259, 1299, 1433
RsaI	GTAC	5 cut(s)	356, 880, 1293, 1439, 1532
RsaNI	GTAC	5 cut(s)	355, 879, 1292, 1438, 1531
RseI	CAYNNNNRTG	4 cut(s)	372, 441, 653, 1722
SacI	GAGCTC	2 cut(s)	837, 1788
SalI	GTCGAC	1 cut(s)	629
SaqAI	TTAA	5 cut(s)	83, 1082, 1265, 1569, 1861
SatI	GCNGC	1 cut(s)	548
SchI	GAGTC	3 cut(s)	571, 1549, 1613
ScrFI	CCNGG	1 cut(s)	173
SduI	GDGCHC	4 cut(s)	837, 1649, 1734, 1788
SfaNI	GCATC	1 cut(s)	952
SfcI	CTRYAG	2 cut(s)	1513, 1760
Sfr274I	CTCGAG	1 cut(s)	1642
SlaI	CTCGAG	1 cut(s)	1642
SmiMI	CAYNNNNRTG	4 cut(s)	372, 441, 653, 1722
SmlI	CTYRAG	7 cut(s)	82, 346, 771, 1472, 1568, 1642, 1781
SmoI	CTYRAG	7 cut(s)	82, 346, 771, 1472, 1568, 1642, 1781
SphI	GCATGC	1 cut(s)	658
SsiI	CCGC	1 cut(s)	827
SspMI	CTAG	1 cut(s)	659
SstI	GAGCTC	2 cut(s)	837, 1788
StyD4I	CCNGG	1 cut(s)	171
TaaI	ACNGT	4 cut(s)	41, 124, 1319, 1535
TaqI	TCGA	5 cut(s)	418, 630, 1008, 1538, 1643
TfiI	GAWTC	7 cut(s)	78, 412, 1056, 1388, 1484, 1601, 1748
Tru1I	TTAA	5 cut(s)	83, 1082, 1265, 1569, 1861
Tru9I	TTAA	5 cut(s)	83, 1082, 1265, 1569, 1861
TscAI	CASTG	1 cut(s)	1471
TseFI	GTSAC	3 cut(s)	667, 1252, 1444
TseI	GCWGC	1 cut(s)	547
Tsp45I	GTSAC	3 cut(s)	667, 1252, 1444
TspGWI	ACGGA	2 cut(s)	338, 1240
TspRI	CASTG	1 cut(s)	1471
Vha464I	CTTAAG	2 cut(s)	82, 1568
XapI	RAATTY	3 cut(s)	50, 584, 1073
XceI	RCATGY	5 cut(s)	182, 652, 658, 1495, 1721
XhoI	CTCGAG	1 cut(s)	1642
XmiI	GTMKAC	1 cut(s)	630
XspI	CTAG	1 cut(s)	659

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.