FvH4_5g12820

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014454
Physical Location & Seq
Forward (+)
7255727 .. 7257626
1900 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12820.t2

Sequence Viewer

Length: 633 bp
ATGGCTTCTCTCTCAAGGAGACTACTCCTCTTTCGCTCTCTCTACCACTCAAACCCCAACAGCGTGCTCTTCACGCGCTTCTGCACCACCGCCTCCTCCTCCTCAGATAGCTCCGACTCAGACAAGCCCGTCACTCCGGACCTAGAGTCCGAATCACGGCCCGACCCGACCCGCCGTGTTTTCCGCAACCAGCCGCTCGAGAAAGGCGTCGACGTCGGCATTTACAAGGCGATACTGGTAGGGCAGGTGGGGCAGACTCCGATGCAGAAGATACTGAAGAGTGGGGCGGCGGTGACCATGTTCTCACTTGGGACCGGCGGGATTCGAAACAACCGGAGGCCTTTGCAAACTGAGAGCCCGACGGAGTATGCCAACCGCTGTGAGGTGCAGTGGCACAGGGTTTGTGTTTATCCGGAGAGGCTCGGCAAGATTGCGGTGCAGAATGCTCCGCCTGGTTCAATTATATATGTGGAAGGCAATCTAGAGACCAAAATCTTCACTGACCCAGTAACTGGGCTTGTACGACGAGTTAGAGAGATTGCTGTTCGGAAAAATGGTCGCCTTGTGTTTTTGGGTAATGGAAATATTGCTGAGCCAGCAGTAAAAGGGATCAGAGGTGTTGGCTACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03430 ko03440 map03030 map03430 map03440
Pfam Domains
Protein Families
SSB

Protein Analysis

211

Amino Acids

23.21

Weight (kDa)

9.91

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 76 - 151 8.7e-08 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014454)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18580
fragaria_vesca FvH4_5g12820 FvH4_5g12820
malus_domestica MD06G1209800.v1.1 MD14G1220600.v1.1
prunus_persica Prupe.5G215900_v2.0.a1
pyrus_communis pycom06g18740
rosa_chinensis RchiOBHm_Chr7g0184411
rosa_laevigata RLG00000004977
rosa_multiflora Rmu_co8454851.1_g000001 Rmu_sc0001557.1_g000018
rosa_roxburghii Rroxscaffold_3G00269650
rosa_samantha Rh7AG080200 Rh7BG067000 Rh7CG067300 Rh7DG067000
rosa_wichuraiana Rw7G005510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 235
AasI GACNNNNNNGTC 1 cut(s) 128
AatII GACGTC 1 cut(s) 216
Acc36I ACCTGC 1 cut(s) 235
AccB7I CCANNNNNTGG 1 cut(s) 512
AccBSI CCGCTC 1 cut(s) 196
AccI GTMKAC 1 cut(s) 210
AccII CGCG 1 cut(s) 76
AccIII TCCGGA 2 cut(s) 136, 412
AclWI GGATC 1 cut(s) 617
AcuI CTGAAG 1 cut(s) 296
AcyI GRCGYC 2 cut(s) 207, 213
AfaI GTAC 1 cut(s) 522
AfiI CCNNNNNNNGG 3 cut(s) 156, 382, 512
AgsI TTSAA 1 cut(s) 459
AhdI GACNNNNNGTC 1 cut(s) 145
AjnI CCWGG 1 cut(s) 451
AluBI AGCT 1 cut(s) 111
AluI AGCT 1 cut(s) 111
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 2 cut(s) 13, 479
AlwI GGATC 1 cut(s) 617
AlwNI CAGNNNCTG 1 cut(s) 512
Ama87I CYCGRG 1 cut(s) 197
Aor13HI TCCGGA 2 cut(s) 136, 412
AoxI GGCC 2 cut(s) 158, 338
AspLEI GCGC 1 cut(s) 78
AspS9I GGNCC 3 cut(s) 139, 159, 312
AsuHPI GGTGA 1 cut(s) 304
AsuII TTCGAA 1 cut(s) 325
AvaI CYCGRG 1 cut(s) 197
AvaII GGWCC 2 cut(s) 139, 312
BanII GRGCYC 1 cut(s) 359
Bbv12I GWGCWC 1 cut(s) 69
BceAI ACGGC 2 cut(s) 159, 173
BciT130I CCWGG 1 cut(s) 453
BcoDI GTCTC 2 cut(s) 13, 479
BfaI CTAG 2 cut(s) 143, 482
BfuAI ACCTGC 1 cut(s) 235
BisI GCNGC 2 cut(s) 194, 288
BlpI GCTNAGC 1 cut(s) 591
BlsI GCNGC 2 cut(s) 195, 289
Bme1390I CCNGG 1 cut(s) 453
Bme18I GGWCC 2 cut(s) 139, 312
BmeRI GACNNNNNGTC 1 cut(s) 145
BmeT110I CYCGRG 1 cut(s) 197
BmgT120I GGNCC 3 cut(s) 139, 159, 312
BmiI GGNNCC 1 cut(s) 313
BmrFI CCNGG 1 cut(s) 453
BmrI ACTGGG 2 cut(s) 500, 522
BmsI GCATC 1 cut(s) 252
BmuI ACTGGG 2 cut(s) 500, 522
Bpu1102I GCTNAGC 1 cut(s) 591
Bpu14I TTCGAA 1 cut(s) 325
BsaHI GRCGYC 2 cut(s) 207, 213
BsaI GGTCTC 1 cut(s) 479
BsaWI WCCGGW 3 cut(s) 136, 333, 412
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 382, 512
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 3 cut(s) 240, 506, 517
BseAI TCCGGA 2 cut(s) 136, 412
BseBI CCWGG 1 cut(s) 453
BseLI CCNNNNNNNGG 3 cut(s) 156, 382, 512
BseMII CTCAG 4 cut(s) 117, 132, 342, 582
BseNI ACTGG 3 cut(s) 240, 506, 517
BseRI GAGGAG 4 cut(s) 17, 85, 88, 91
BsgI GTGCAG 3 cut(s) 67, 407, 458
Bsh1236I CGCG 1 cut(s) 76
BshFI GGCC 2 cut(s) 160, 340
BsiHKAI GWGCWC 1 cut(s) 69
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 4 cut(s) 137, 315, 334, 413
BslFI GGGAC 1 cut(s) 325
BslI CCNNNNNNNGG 3 cut(s) 156, 382, 512
BsmAI GTCTC 2 cut(s) 13, 479
BsmFI GGGAC 1 cut(s) 325
BsmI GAATGC 1 cut(s) 448
BsnI GGCC 2 cut(s) 160, 340
Bso31I GGTCTC 1 cut(s) 479
BsoBI CYCGRG 1 cut(s) 197
Bsp119I TTCGAA 1 cut(s) 325
Bsp1286I GDGCHC 2 cut(s) 69, 359
Bsp13I TCCGGA 2 cut(s) 136, 412
Bsp143I GATC 1 cut(s) 609
Bsp1720I GCTNAGC 1 cut(s) 591
BspANI GGCC 2 cut(s) 160, 340
BspCNI CTCAG 4 cut(s) 116, 131, 343, 583
BspEI TCCGGA 2 cut(s) 136, 412
BspFNI CGCG 1 cut(s) 76
BspLI GGNNCC 1 cut(s) 313
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 1 cut(s) 617
BspQI GCTCTTC 1 cut(s) 74
BspT104I TTCGAA 1 cut(s) 325
BspTNI GGTCTC 1 cut(s) 479
BsrBI CCGCTC 1 cut(s) 196
BsrFI RCCGGY 1 cut(s) 314
BsrI ACTGG 3 cut(s) 240, 506, 517
BssAI RCCGGY 1 cut(s) 314
BssMI GATC 1 cut(s) 609
BssNI GRCGYC 2 cut(s) 207, 213
Bst2UI CCWGG 1 cut(s) 453
Bst6I CTCTTC 2 cut(s) 74, 272
BstACI GRCGYC 2 cut(s) 207, 213
BstBI TTCGAA 1 cut(s) 325
BstC8I GCNNGC 2 cut(s) 65, 597
BstDEI CTNAG 4 cut(s) 103, 118, 351, 591
BstEII GGTNACC 1 cut(s) 292
BstFNI CGCG 1 cut(s) 76
BstHHI GCGC 1 cut(s) 78
BstKTI GATC 1 cut(s) 612
BstMAI GTCTC 2 cut(s) 13, 479
BstMBI GATC 1 cut(s) 609
BstMWI GCNNNNNNNGC 3 cut(s) 73, 250, 596
BstNI CCWGG 1 cut(s) 453
BstPI GGTNACC 1 cut(s) 292
BstSCI CCNGG 1 cut(s) 451
BstUI CGCG 1 cut(s) 76
BsuRI GGCC 2 cut(s) 160, 340
BtsI GCAGTG 1 cut(s) 395
BtsIMutI CAGTG 2 cut(s) 395, 498
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 2 cut(s) 65, 597
CaiI CAGNNNCTG 1 cut(s) 512
CfoI GCGC 1 cut(s) 78
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 3 cut(s) 139, 159, 312
CseI GACGC 1 cut(s) 196
Csp6I GTAC 1 cut(s) 521
CviAII CATG 1 cut(s) 298
CviQI GTAC 1 cut(s) 521
DdeI CTNAG 4 cut(s) 103, 118, 351, 591
DpnI GATC 1 cut(s) 611
DpnII GATC 1 cut(s) 609
DrdI GACNNNNNNGTC 1 cut(s) 128
DriI GACNNNNNGTC 1 cut(s) 145
DseDI GACNNNNNNGTC 1 cut(s) 128
Eam1104I CTCTTC 2 cut(s) 74, 272
Eam1105I GACNNNNNGTC 1 cut(s) 145
EarI CTCTTC 2 cut(s) 74, 272
EciI GGCGGA 1 cut(s) 438
Eco147I AGGCCT 1 cut(s) 340
Eco24I GRGCYC 1 cut(s) 359
Eco31I GGTCTC 1 cut(s) 479
Eco47I GGWCC 2 cut(s) 139, 312
Eco57I CTGAAG 1 cut(s) 296
Eco88I CYCGRG 1 cut(s) 197
Eco91I GGTNACC 1 cut(s) 292
EcoO65I GGTNACC 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 451
EcoT38I GRGCYC 1 cut(s) 359
FaeI CATG 1 cut(s) 301
FaiI YATR 5 cut(s) 299, 369, 464, 466, 468
FaqI GGGAC 1 cut(s) 325
FatI CATG 1 cut(s) 297
FauI CCCGC 2 cut(s) 179, 311
FblI GTMKAC 1 cut(s) 210
Fnu4HI GCNGC 2 cut(s) 194, 288
FriOI GRGCYC 1 cut(s) 359
Fsp4HI GCNGC 2 cut(s) 194, 288
FspBI CTAG 2 cut(s) 143, 482
GlaI GCGC 1 cut(s) 77
GluI GCNGC 2 cut(s) 194, 288
HaeIII GGCC 2 cut(s) 160, 340
HapII CCGG 4 cut(s) 137, 315, 334, 413
HgaI GACGC 1 cut(s) 196
HhaI GCGC 1 cut(s) 78
Hin1I GRCGYC 2 cut(s) 207, 213
Hin1II CATG 1 cut(s) 301
Hin6I GCGC 1 cut(s) 76
HinP1I GCGC 1 cut(s) 76
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 5 cut(s) 116, 146, 152, 256, 322
HpaII CCGG 4 cut(s) 137, 315, 334, 413
HphI GGTGA 1 cut(s) 304
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 7 cut(s) 106, 115, 121, 151, 261, 549, 614
Hpy188III TCNNGA 4 cut(s) 137, 199, 413, 482
Hpy8I GTNNAC 1 cut(s) 211
Hpy99I CGWCG 5 cut(s) 212, 215, 218, 364, 528
HpyAV CCTTC 1 cut(s) 467
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 5 cut(s) 84, 265, 346, 388, 439
HpyF10VI GCNNNNNNNGC 3 cut(s) 73, 250, 596
HpyF3I CTNAG 4 cut(s) 103, 118, 351, 591
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 2 cut(s) 207, 213
Hsp92II CATG 1 cut(s) 301
HspAI GCGC 1 cut(s) 76
Kpn2I TCCGGA 2 cut(s) 136, 412
Kzo9I GATC 1 cut(s) 609
LguI GCTCTTC 1 cut(s) 74
LmnI GCTCC 2 cut(s) 116, 451
LweI GCATC 1 cut(s) 252
MaeI CTAG 2 cut(s) 143, 482
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 3 cut(s) 130, 292, 508
MalI GATC 1 cut(s) 611
MbiI CCGCTC 1 cut(s) 196
MboI GATC 1 cut(s) 609
MboII GAAGA 4 cut(s) 61, 280, 289, 487
MhlI GDGCHC 2 cut(s) 69, 359
MluCI AATT 1 cut(s) 459
MlyI GAGTC 3 cut(s) 110, 155, 250
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 9 cut(s) 38, 103, 106, 109, 112, 330, 376, 411, 608
MroI TCCGGA 2 cut(s) 136, 412
MspA1I CMGCKG 1 cut(s) 378
MspI CCGG 4 cut(s) 137, 315, 334, 413
MspR9I CCNGG 1 cut(s) 453
Mva1269I GAATGC 1 cut(s) 448
MvaI CCWGG 1 cut(s) 453
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 3 cut(s) 73, 250, 596
NdeII GATC 1 cut(s) 609
NlaIII CATG 1 cut(s) 301
NlaIV GGNNCC 1 cut(s) 313
NmeAIII GCCGAG 1 cut(s) 402
NmuCI GTSAC 2 cut(s) 130, 292
NspV TTCGAA 1 cut(s) 325
PaeR7I CTCGAG 1 cut(s) 197
PaqCI CACCTGC 1 cut(s) 235
PceI AGGCCT 1 cut(s) 340
PciSI GCTCTTC 1 cut(s) 74
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PctI GAATGC 1 cut(s) 448
PfeI GAWTC 2 cut(s) 152, 322
PflMI CCANNNNNTGG 1 cut(s) 512
PkrI GCNGC 2 cut(s) 195, 289
PleI GAGTC 3 cut(s) 110, 154, 250
PpsI GAGTC 3 cut(s) 110, 154, 250
Psp6I CCWGG 1 cut(s) 451
PspEI GGTNACC 1 cut(s) 292
PspGI CCWGG 1 cut(s) 451
PspN4I GGNNCC 1 cut(s) 313
PspPI GGNCC 3 cut(s) 139, 159, 312
PstNI CAGNNNCTG 1 cut(s) 512
RsaI GTAC 1 cut(s) 522
RsaNI GTAC 1 cut(s) 521
SalI GTCGAC 1 cut(s) 209
SapI GCTCTTC 1 cut(s) 74
SatI GCNGC 2 cut(s) 194, 288
Sau3AI GATC 1 cut(s) 609
Sau96I GGNCC 3 cut(s) 139, 159, 312
SchI GAGTC 3 cut(s) 110, 155, 250
ScrFI CCNGG 1 cut(s) 453
SduI GDGCHC 2 cut(s) 69, 359
SetI ASST 6 cut(s) 113, 144, 216, 249, 387, 619
SfaNI GCATC 1 cut(s) 252
Sfr274I CTCGAG 1 cut(s) 197
SfuI TTCGAA 1 cut(s) 325
SgrDI CGTCGACG 1 cut(s) 209
SinI GGWCC 2 cut(s) 139, 312
SlaI CTCGAG 1 cut(s) 197
SmlI CTYRAG 2 cut(s) 13, 197
SmoI CTYRAG 2 cut(s) 13, 197
Sse9I AATT 1 cut(s) 459
SseBI AGGCCT 1 cut(s) 340
SspI AATATT 1 cut(s) 586
SspMI CTAG 2 cut(s) 143, 482
StuI AGGCCT 1 cut(s) 340
StyD4I CCNGG 1 cut(s) 451
TaiI ACGT 1 cut(s) 216
TaqI TCGA 3 cut(s) 198, 210, 325
TasI AATT 1 cut(s) 459
TauI GCSGC 2 cut(s) 196, 290
TfiI GAWTC 2 cut(s) 152, 322
TscAI CASTG 2 cut(s) 395, 505
TseFI GTSAC 2 cut(s) 130, 292
Tsp45I GTSAC 2 cut(s) 130, 292
TspGWI ACGGA 1 cut(s) 377
TspRI CASTG 2 cut(s) 395, 505
Van91I CCANNNNNTGG 1 cut(s) 512
VpaK11BI GGWCC 2 cut(s) 139, 312
XbaI TCTAGA 1 cut(s) 481
XhoI CTCGAG 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 210
XspI CTAG 2 cut(s) 143, 482
ZraI GACGTC 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.