Rw7G005510

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014454
Physical Location & Seq
Reverse (-)
4631694 .. 4633323
1630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G005510.1

Sequence Viewer

Length: 669 bp
ATGTCAGCCTCCTCCTCCATGGCTTCACTCTCAAGGAGACTACTCCTCTCTCGCTCTCTCTACCACTCAAACCCCAGCACCGTGCTCTCCACGCGCTTCTGCACCACCACCAGCACGACCCCCCACGTCACCTCCGACTCAGACGAGCCCATCACCTCGGACCTAGAGTCCGACCCGACCCCATCACAGCCCTCCGCCCCCAACCCGGTCCGCGGTATCTACGATCAGCCGCTCGAGAACGGAGTCGACGTCGGCATTTATAAGGCGATACTGGTGGGGCAGGTGGGGCAGAGTCCGTTGCAGAAGAGACTGAAGAGTGGGGCGCAGGTGACCATGTTCTCAATTGGGACCGGCGGCATTCGAAACAACCGGAGGCCCTTTGAGACCGAGAACCCGACGGAGTATGCCAACCGCTGTGTGGTTCAGTGGCACAGGGTTTGTGTTTATCCGGAGAGGCTCGGCAAGATTGCGGTGCAGAATGCTTTGCCTGGTTCAATTTTATACTTGGAAGGTAATCTGGAGACCAAAATCTTCACTGACCCAACAACTGGGCTTGTTCGACGAGTTAGAGAGATTGCTATTCGCAGAAATGGTCGCCTTGTGTTTTTGGGTAAAGGAAACGATGCTGAGCCAACAGTAACGGGGCTCAGAGGTGTTGGCTACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03430 ko03440 map03030 map03430 map03440
Pfam Domains
Protein Families
SSB

Protein Analysis

222

Amino Acids

24.28

Weight (kDa)

9.43

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 88 - 195 5.5e-14 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014454)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18580
fragaria_vesca FvH4_5g12820 FvH4_5g12820
malus_domestica MD06G1209800.v1.1 MD14G1220600.v1.1
prunus_persica Prupe.5G215900_v2.0.a1
pyrus_communis pycom06g18740
rosa_chinensis RchiOBHm_Chr7g0184411
rosa_laevigata RLG00000004977
rosa_multiflora Rmu_co8454851.1_g000001 Rmu_sc0001557.1_g000018
rosa_roxburghii Rroxscaffold_3G00269650
rosa_samantha Rh7AG080200 Rh7BG067000 Rh7CG067300 Rh7DG067000
rosa_wichuraiana Rw7G005510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 261
AarI CACCTGC 2 cut(s) 271, 316
AatII GACGTC 1 cut(s) 252
Acc36I ACCTGC 2 cut(s) 271, 316
AccB7I CCANNNNNTGG 1 cut(s) 548
AccBSI CCGCTC 1 cut(s) 232
AccI GTMKAC 1 cut(s) 246
AccII CGCG 2 cut(s) 94, 213
AccIII TCCGGA 1 cut(s) 448
AciI CCGC 7 cut(s) 195, 211, 213, 230, 354, 412, 470
AcuI CTGAAG 1 cut(s) 332
AcyI GRCGYC 1 cut(s) 249
AfiI CCNNNNNNNGG 4 cut(s) 205, 212, 418, 548
AgsI TTSAA 1 cut(s) 495
AhdI GACNNNNNGTC 1 cut(s) 166
AjiI CACGTC 1 cut(s) 127
AjnI CCWGG 1 cut(s) 487
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 4 cut(s) 31, 301, 377, 515
Ama87I CYCGRG 1 cut(s) 233
Aor13HI TCCGGA 1 cut(s) 448
AoxI GGCC 1 cut(s) 374
AspLEI GCGC 2 cut(s) 96, 325
AspS9I GGNCC 4 cut(s) 160, 208, 348, 375
AsuC2I CCSGG 1 cut(s) 206
AsuHPI GGTGA 3 cut(s) 121, 145, 340
AsuII TTCGAA 1 cut(s) 361
AvaI CYCGRG 1 cut(s) 233
AvaII GGWCC 3 cut(s) 160, 208, 348
BanII GRGCYC 2 cut(s) 150, 648
Bbv12I GWGCWC 1 cut(s) 87
BccI CCATC 2 cut(s) 158, 190
BciT130I CCWGG 1 cut(s) 489
BcnI CCSGG 1 cut(s) 206
BcoDI GTCTC 4 cut(s) 31, 301, 377, 515
BfaI CTAG 1 cut(s) 164
BfuAI ACCTGC 2 cut(s) 271, 316
BisI GCNGC 2 cut(s) 230, 355
BlpI GCTNAGC 1 cut(s) 627
BlsI GCNGC 2 cut(s) 231, 356
Bme1390I CCNGG 2 cut(s) 206, 489
Bme18I GGWCC 3 cut(s) 160, 208, 348
BmeRI GACNNNNNGTC 1 cut(s) 166
BmeT110I CYCGRG 1 cut(s) 233
BmgBI CACGTC 1 cut(s) 127
BmgT120I GGNCC 4 cut(s) 160, 208, 348, 375
BmiI GGNNCC 1 cut(s) 349
BmrFI CCNGG 2 cut(s) 206, 489
BmrI ACTGGG 1 cut(s) 558
BmsI GCATC 1 cut(s) 613
BmuI ACTGGG 1 cut(s) 558
BpmI CTGGAG 1 cut(s) 539
Bpu1102I GCTNAGC 1 cut(s) 627
Bpu14I TTCGAA 1 cut(s) 361
BpuEI CTTGAG 1 cut(s) 16
BpuMI CCSGG 1 cut(s) 206
BsaHI GRCGYC 1 cut(s) 249
BsaI GGTCTC 2 cut(s) 377, 515
BsaJI CCNNGG 3 cut(s) 18, 156, 211
BsaWI WCCGGW 2 cut(s) 369, 448
BsaXI ACNNNNNCTCC 4 cut(s) 116, 146, 234, 264
Bsc4I CCNNNNNNNGG 4 cut(s) 205, 212, 418, 548
Bse118I RCCGGY 1 cut(s) 350
Bse1I ACTGG 2 cut(s) 276, 553
BseAI TCCGGA 1 cut(s) 448
BseBI CCWGG 1 cut(s) 489
BseDI CCNNGG 3 cut(s) 18, 156, 211
BseLI CCNNNNNNNGG 4 cut(s) 205, 212, 418, 548
BseMII CTCAG 3 cut(s) 153, 618, 661
BseNI ACTGG 2 cut(s) 276, 553
BseRI GAGGAG 2 cut(s) 4, 35
BseYI CCCAGC 1 cut(s) 74
BsgI GTGCAG 2 cut(s) 85, 494
Bsh1236I CGCG 2 cut(s) 94, 213
BshFI GGCC 1 cut(s) 376
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 4 cut(s) 206, 351, 370, 449
BslFI GGGAC 1 cut(s) 361
BslI CCNNNNNNNGG 4 cut(s) 205, 212, 418, 548
BsmAI GTCTC 4 cut(s) 31, 301, 377, 515
BsmFI GGGAC 1 cut(s) 361
BsmI GAATGC 2 cut(s) 357, 484
BsnI GGCC 1 cut(s) 376
Bso31I GGTCTC 2 cut(s) 377, 515
BsoBI CYCGRG 1 cut(s) 233
Bsp119I TTCGAA 1 cut(s) 361
Bsp1286I GDGCHC 3 cut(s) 87, 150, 648
Bsp13I TCCGGA 1 cut(s) 448
Bsp143I GATC 1 cut(s) 223
Bsp1720I GCTNAGC 1 cut(s) 627
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 7 cut(s) 195, 211, 213, 230, 354, 412, 470
BspANI GGCC 1 cut(s) 376
BspCNI CTCAG 3 cut(s) 152, 619, 660
BspEI TCCGGA 1 cut(s) 448
BspFNI CGCG 2 cut(s) 94, 213
BspLI GGNNCC 1 cut(s) 349
BspMI ACCTGC 2 cut(s) 271, 316
BspT104I TTCGAA 1 cut(s) 361
BspTNI GGTCTC 2 cut(s) 377, 515
BsrBI CCGCTC 1 cut(s) 232
BsrFI RCCGGY 1 cut(s) 350
BsrI ACTGG 2 cut(s) 276, 553
BssAI RCCGGY 1 cut(s) 350
BssECI CCNNGG 3 cut(s) 18, 156, 211
BssMI GATC 1 cut(s) 223
BssNI GRCGYC 1 cut(s) 249
BssT1I CCWWGG 1 cut(s) 18
Bst2UI CCWGG 1 cut(s) 489
Bst4CI ACNGT 2 cut(s) 82, 637
Bst6I CTCTTC 2 cut(s) 299, 308
BstACI GRCGYC 1 cut(s) 249
BstBI TTCGAA 1 cut(s) 361
BstDEI CTNAG 3 cut(s) 139, 627, 647
BstDSI CCRYGG 2 cut(s) 18, 211
BstEII GGTNACC 1 cut(s) 328
BstFNI CGCG 2 cut(s) 94, 213
BstHHI GCGC 2 cut(s) 96, 325
BstKTI GATC 1 cut(s) 226
BstMAI GTCTC 4 cut(s) 31, 301, 377, 515
BstMBI GATC 1 cut(s) 223
BstMWI GCNNNNNNNGC 2 cut(s) 91, 286
BstNI CCWGG 1 cut(s) 489
BstPI GGTNACC 1 cut(s) 328
BstSCI CCNGG 2 cut(s) 204, 487
BstUI CGCG 2 cut(s) 94, 213
BsuRI GGCC 1 cut(s) 376
BtgI CCRYGG 2 cut(s) 18, 211
BtrI CACGTC 1 cut(s) 127
BtsIMutI CAGTG 2 cut(s) 431, 534
BveI ACCTGC 2 cut(s) 271, 316
CfoI GCGC 2 cut(s) 96, 325
Cfr10I RCCGGY 1 cut(s) 350
Cfr13I GGNCC 4 cut(s) 160, 208, 348, 375
Cfr42I CCGCGG 1 cut(s) 214
CpoI CGGWCCG 1 cut(s) 208
CspI CGGWCCG 1 cut(s) 208
CviAII CATG 2 cut(s) 19, 334
DdeI CTNAG 3 cut(s) 139, 627, 647
DpnI GATC 1 cut(s) 225
DpnII GATC 1 cut(s) 223
DriI GACNNNNNGTC 1 cut(s) 166
Eam1104I CTCTTC 2 cut(s) 299, 308
Eam1105I GACNNNNNGTC 1 cut(s) 166
EarI CTCTTC 2 cut(s) 299, 308
EciI GGCGGA 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 18
Eco24I GRGCYC 2 cut(s) 150, 648
Eco31I GGTCTC 2 cut(s) 377, 515
Eco47I GGWCC 3 cut(s) 160, 208, 348
Eco57I CTGAAG 1 cut(s) 332
Eco88I CYCGRG 1 cut(s) 233
Eco91I GGTNACC 1 cut(s) 328
EcoO109I RGGNCCY 1 cut(s) 375
EcoO65I GGTNACC 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 487
EcoT14I CCWWGG 1 cut(s) 18
EcoT38I GRGCYC 2 cut(s) 150, 648
ErhI CCWWGG 1 cut(s) 18
FaeI CATG 2 cut(s) 22, 337
FaiI YATR 5 cut(s) 20, 261, 335, 405, 502
FaqI GGGAC 1 cut(s) 361
FatI CATG 2 cut(s) 18, 333
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 2 cut(s) 230, 355
FriOI GRGCYC 2 cut(s) 150, 648
Fsp4HI GCNGC 2 cut(s) 230, 355
FspBI CTAG 1 cut(s) 164
GlaI GCGC 2 cut(s) 95, 324
GluI GCNGC 2 cut(s) 230, 355
GsaI CCCAGC 1 cut(s) 78
GsuI CTGGAG 1 cut(s) 539
HaeIII GGCC 1 cut(s) 376
HapII CCGG 4 cut(s) 206, 351, 370, 449
HhaI GCGC 2 cut(s) 96, 325
Hin1I GRCGYC 1 cut(s) 249
Hin1II CATG 2 cut(s) 22, 337
Hin6I GCGC 2 cut(s) 94, 323
HinP1I GCGC 2 cut(s) 94, 323
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 4 cut(s) 137, 167, 243, 292
HpaII CCGG 4 cut(s) 206, 351, 370, 449
HphI GGTGA 3 cut(s) 121, 145, 340
Hpy166II GTNNAC 1 cut(s) 247
Hpy188I TCNGA 6 cut(s) 136, 142, 160, 172, 650, 668
Hpy188III TCNNGA 3 cut(s) 235, 449, 518
Hpy8I GTNNAC 1 cut(s) 247
Hpy99I CGWCG 4 cut(s) 251, 254, 400, 564
HpyAV CCTTC 1 cut(s) 503
HpyCH4III ACNGT 2 cut(s) 82, 637
HpyCH4IV ACGT 2 cut(s) 126, 249
HpyCH4V TGCA 3 cut(s) 102, 301, 475
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 286
HpyF3I CTNAG 3 cut(s) 139, 627, 647
HpySE526I ACGT 2 cut(s) 126, 249
Hsp92I GRCGYC 1 cut(s) 249
Hsp92II CATG 2 cut(s) 22, 337
HspAI GCGC 2 cut(s) 94, 323
Kpn2I TCCGGA 1 cut(s) 448
KspI CCGCGG 1 cut(s) 214
Kzo9I GATC 1 cut(s) 223
LweI GCATC 1 cut(s) 613
MaeI CTAG 1 cut(s) 164
MaeII ACGT 2 cut(s) 126, 249
MaeIII GTNAC 3 cut(s) 127, 328, 637
MalI GATC 1 cut(s) 225
MbiI CCGCTC 1 cut(s) 232
MboI GATC 1 cut(s) 223
MboII GAAGA 3 cut(s) 316, 325, 523
MfeI CAATTG 1 cut(s) 342
MhlI GDGCHC 3 cut(s) 87, 150, 648
MluCI AATT 2 cut(s) 342, 495
MlyI GAGTC 4 cut(s) 131, 176, 252, 301
MmeI TCCRAC 2 cut(s) 159, 195
MroI TCCGGA 1 cut(s) 448
MspA1I CMGCKG 2 cut(s) 213, 414
MspI CCGG 4 cut(s) 206, 351, 370, 449
MspR9I CCNGG 2 cut(s) 206, 489
MunI CAATTG 1 cut(s) 342
Mva1269I GAATGC 2 cut(s) 357, 484
MvaI CCWGG 1 cut(s) 489
MvnI CGCG 2 cut(s) 94, 213
MwoI GCNNNNNNNGC 2 cut(s) 91, 286
NciI CCSGG 1 cut(s) 206
NcoI CCATGG 1 cut(s) 18
NdeII GATC 1 cut(s) 223
NlaIII CATG 2 cut(s) 22, 337
NlaIV GGNNCC 1 cut(s) 349
NmeAIII GCCGAG 1 cut(s) 438
NmuCI GTSAC 2 cut(s) 127, 328
NspV TTCGAA 1 cut(s) 361
PaeR7I CTCGAG 1 cut(s) 233
PaqCI CACCTGC 2 cut(s) 271, 316
PcsI WCGNNNNNNNCGW 2 cut(s) 132, 246
PctI GAATGC 2 cut(s) 357, 484
PflMI CCANNNNNTGG 1 cut(s) 548
PkrI GCNGC 2 cut(s) 231, 356
PleI GAGTC 4 cut(s) 131, 175, 251, 300
PpsI GAGTC 4 cut(s) 131, 175, 251, 300
PsiI TTATAA 1 cut(s) 261
Psp6I CCWGG 1 cut(s) 487
PspEI GGTNACC 1 cut(s) 328
PspFI CCCAGC 1 cut(s) 74
PspGI CCWGG 1 cut(s) 487
PspN4I GGNNCC 1 cut(s) 349
PspPI GGNCC 4 cut(s) 160, 208, 348, 375
Rsr2I CGGWCCG 1 cut(s) 208
RsrII CGGWCCG 1 cut(s) 208
SacII CCGCGG 1 cut(s) 214
SalI GTCGAC 1 cut(s) 245
SatI GCNGC 2 cut(s) 230, 355
Sau3AI GATC 1 cut(s) 223
Sau96I GGNCC 4 cut(s) 160, 208, 348, 375
SchI GAGTC 4 cut(s) 131, 176, 252, 301
ScrFI CCNGG 2 cut(s) 206, 489
SduI GDGCHC 3 cut(s) 87, 150, 648
SetI ASST 9 cut(s) 129, 134, 158, 165, 252, 285, 330, 514, 655
SfaNI GCATC 1 cut(s) 613
Sfr274I CTCGAG 1 cut(s) 233
Sfr303I CCGCGG 1 cut(s) 214
SfuI TTCGAA 1 cut(s) 361
SgrBI CCGCGG 1 cut(s) 214
SinI GGWCC 3 cut(s) 160, 208, 348
SlaI CTCGAG 1 cut(s) 233
SmlI CTYRAG 2 cut(s) 31, 233
SmoI CTYRAG 2 cut(s) 31, 233
Sse9I AATT 2 cut(s) 342, 495
SsiI CCGC 7 cut(s) 195, 211, 213, 230, 354, 412, 470
SspMI CTAG 1 cut(s) 164
StyD4I CCNGG 2 cut(s) 204, 487
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 2 cut(s) 82, 637
TaiI ACGT 2 cut(s) 129, 252
TaqI TCGA 4 cut(s) 234, 246, 361, 559
TaqII GACCGA 1 cut(s) 401
TasI AATT 2 cut(s) 342, 495
TauI GCSGC 2 cut(s) 232, 357
TscAI CASTG 2 cut(s) 431, 541
TseFI GTSAC 2 cut(s) 127, 328
Tsp45I GTSAC 2 cut(s) 127, 328
TspGWI ACGGA 3 cut(s) 255, 285, 413
TspRI CASTG 2 cut(s) 431, 541
Van91I CCANNNNNTGG 1 cut(s) 548
VpaK11BI GGWCC 3 cut(s) 160, 208, 348
XcmI CCANNNNNNNNNTGG 1 cut(s) 415
XhoI CTCGAG 1 cut(s) 233
XmiI GTMKAC 1 cut(s) 246
XspI CTAG 1 cut(s) 164
ZraI GACGTC 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.