Rroxscaffold_3G00269650

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014454
Physical Location & Seq
Forward (+)
62466301 .. 62468021
1721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00269650.1

Sequence Viewer

Length: 666 bp
ATGACAGCCTCCTCCTCCATGGCTTCTCTCTCAAGGAGACTACTCCTCTCTCGCTCTCTCTACCACTCAAACCCCAGCACCGTGCTCTCCACGCGCTTCTGCACCACCACCACCACCACCCACGTTACCTCCGACGCAGACGAGCCCATCACCTCGGACCTTGAGTCCGACCCGACCCCATCACAGCCTTCCGCCCCCAACCCGGTCCGCGGTATCTACGATCAGCCGCTCGAGAACGGAGTCGACGTCGGCATTTATAAGGCGATACTGGTGGGGCAGGTGGGGCAGAGTCCGTTGCAGAAGAGACTGAAGAGTGGGGCACAGGTGACCATGTTCTCAATTGGGACCGGCGGCATTCGAAACAACCGGAGGCCCTTTGAGACCGAGAACCCGACGGAGTATGCCAACCGCTGTGTGGTTCAGTGGCACAGGGTTTGTGTTTATCCGGAGAGGCTCGGCAAGATTGCGGTGCAGAATGCTTTGCCTGGTTCAATTTTATACTTGGAAGGTAATCTGGAGACCAAAATCTTCACTGACCCAACAACTGGGCTTGTTCGACGAGTTAGAGAGATTGCTATTCGCAGAAATGGTCGCCTTGTGTTTTTGGGTAAAGGAAATGATGCTGAGCCAACAGTAACGGGGCTCAGAGGTGTTGGCTACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03430 ko03440 map03030 map03430 map03440
Pfam Domains
Protein Families
SSB

Protein Analysis

221

Amino Acids

24.2

Weight (kDa)

9.43

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 87 - 194 5.4e-14 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014454)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18580
fragaria_vesca FvH4_5g12820 FvH4_5g12820
malus_domestica MD06G1209800.v1.1 MD14G1220600.v1.1
prunus_persica Prupe.5G215900_v2.0.a1
pyrus_communis pycom06g18740
rosa_chinensis RchiOBHm_Chr7g0184411
rosa_laevigata RLG00000004977
rosa_multiflora Rmu_co8454851.1_g000001 Rmu_sc0001557.1_g000018
rosa_roxburghii Rroxscaffold_3G00269650
rosa_samantha Rh7AG080200 Rh7BG067000 Rh7CG067300 Rh7DG067000
rosa_wichuraiana Rw7G005510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 258
AarI CACCTGC 1 cut(s) 268
AatII GACGTC 1 cut(s) 249
Acc36I ACCTGC 1 cut(s) 268
AccB7I CCANNNNNTGG 1 cut(s) 545
AccBSI CCGCTC 1 cut(s) 229
AccI GTMKAC 1 cut(s) 243
AccII CGCG 2 cut(s) 94, 210
AccIII TCCGGA 1 cut(s) 445
AciI CCGC 7 cut(s) 192, 208, 210, 227, 351, 409, 467
AcuI CTGAAG 1 cut(s) 329
AcyI GRCGYC 1 cut(s) 246
AfiI CCNNNNNNNGG 4 cut(s) 202, 209, 415, 545
AgsI TTSAA 1 cut(s) 492
AhdI GACNNNNNGTC 1 cut(s) 163
AjnI CCWGG 1 cut(s) 484
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 4 cut(s) 31, 298, 374, 512
Ama87I CYCGRG 1 cut(s) 230
Aor13HI TCCGGA 1 cut(s) 445
AoxI GGCC 1 cut(s) 371
AspLEI GCGC 1 cut(s) 96
AspS9I GGNCC 4 cut(s) 157, 205, 345, 372
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 2 cut(s) 142, 337
AsuII TTCGAA 1 cut(s) 358
AvaI CYCGRG 1 cut(s) 230
AvaII GGWCC 3 cut(s) 157, 205, 345
BaeGI GKGCMC 1 cut(s) 322
BanII GRGCYC 2 cut(s) 147, 645
Bbv12I GWGCWC 1 cut(s) 87
BccI CCATC 2 cut(s) 155, 187
BciT130I CCWGG 1 cut(s) 486
BcnI CCSGG 1 cut(s) 203
BcoDI GTCTC 4 cut(s) 31, 298, 374, 512
BfuAI ACCTGC 1 cut(s) 268
BisI GCNGC 2 cut(s) 227, 352
BlpI GCTNAGC 1 cut(s) 624
BlsI GCNGC 2 cut(s) 228, 353
Bme1390I CCNGG 2 cut(s) 203, 486
Bme18I GGWCC 3 cut(s) 157, 205, 345
BmeRI GACNNNNNGTC 1 cut(s) 163
BmeT110I CYCGRG 1 cut(s) 230
BmgT120I GGNCC 4 cut(s) 157, 205, 345, 372
BmiI GGNNCC 1 cut(s) 346
BmrFI CCNGG 2 cut(s) 203, 486
BmrI ACTGGG 1 cut(s) 555
BmsI GCATC 1 cut(s) 610
BmuI ACTGGG 1 cut(s) 555
BpmI CTGGAG 1 cut(s) 536
Bpu1102I GCTNAGC 1 cut(s) 624
Bpu14I TTCGAA 1 cut(s) 358
BpuEI CTTGAG 2 cut(s) 16, 182
BpuMI CCSGG 1 cut(s) 203
BsaHI GRCGYC 1 cut(s) 246
BsaI GGTCTC 2 cut(s) 374, 512
BsaJI CCNNGG 3 cut(s) 18, 153, 208
BsaWI WCCGGW 2 cut(s) 366, 445
BsaXI ACNNNNNCTCC 4 cut(s) 113, 143, 231, 261
Bsc4I CCNNNNNNNGG 4 cut(s) 202, 209, 415, 545
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 2 cut(s) 273, 550
BseAI TCCGGA 1 cut(s) 445
BseBI CCWGG 1 cut(s) 486
BseDI CCNNGG 3 cut(s) 18, 153, 208
BseLI CCNNNNNNNGG 4 cut(s) 202, 209, 415, 545
BseMII CTCAG 2 cut(s) 615, 658
BseNI ACTGG 2 cut(s) 273, 550
BseRI GAGGAG 2 cut(s) 4, 35
BseSI GKGCMC 1 cut(s) 322
BseYI CCCAGC 1 cut(s) 74
BsgI GTGCAG 2 cut(s) 85, 491
Bsh1236I CGCG 2 cut(s) 94, 210
BshFI GGCC 1 cut(s) 373
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 4 cut(s) 203, 348, 367, 446
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 4 cut(s) 202, 209, 415, 545
BsmAI GTCTC 4 cut(s) 31, 298, 374, 512
BsmFI GGGAC 1 cut(s) 358
BsmI GAATGC 2 cut(s) 354, 481
BsnI GGCC 1 cut(s) 373
Bso31I GGTCTC 2 cut(s) 374, 512
BsoBI CYCGRG 1 cut(s) 230
Bsp119I TTCGAA 1 cut(s) 358
Bsp1286I GDGCHC 4 cut(s) 87, 147, 322, 645
Bsp13I TCCGGA 1 cut(s) 445
Bsp143I GATC 1 cut(s) 220
Bsp1720I GCTNAGC 1 cut(s) 624
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 7 cut(s) 192, 208, 210, 227, 351, 409, 467
BspANI GGCC 1 cut(s) 373
BspCNI CTCAG 2 cut(s) 616, 657
BspEI TCCGGA 1 cut(s) 445
BspFNI CGCG 2 cut(s) 94, 210
BspLI GGNNCC 1 cut(s) 346
BspMI ACCTGC 1 cut(s) 268
BspT104I TTCGAA 1 cut(s) 358
BspTNI GGTCTC 2 cut(s) 374, 512
BsrBI CCGCTC 1 cut(s) 229
BsrFI RCCGGY 1 cut(s) 347
BsrI ACTGG 2 cut(s) 273, 550
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 3 cut(s) 18, 153, 208
BssMI GATC 1 cut(s) 220
BssNI GRCGYC 1 cut(s) 246
BssT1I CCWWGG 1 cut(s) 18
Bst2UI CCWGG 1 cut(s) 486
Bst4CI ACNGT 2 cut(s) 82, 634
Bst6I CTCTTC 2 cut(s) 296, 305
BstACI GRCGYC 1 cut(s) 246
BstBI TTCGAA 1 cut(s) 358
BstDEI CTNAG 2 cut(s) 624, 644
BstDSI CCRYGG 2 cut(s) 18, 208
BstEII GGTNACC 1 cut(s) 325
BstFNI CGCG 2 cut(s) 94, 210
BstHHI GCGC 1 cut(s) 96
BstKTI GATC 1 cut(s) 223
BstMAI GTCTC 4 cut(s) 31, 298, 374, 512
BstMBI GATC 1 cut(s) 220
BstMWI GCNNNNNNNGC 2 cut(s) 91, 283
BstNI CCWGG 1 cut(s) 486
BstPI GGTNACC 1 cut(s) 325
BstSCI CCNGG 2 cut(s) 201, 484
BstSLI GKGCMC 1 cut(s) 322
BstUI CGCG 2 cut(s) 94, 210
BsuRI GGCC 1 cut(s) 373
BtgI CCRYGG 2 cut(s) 18, 208
BtsIMutI CAGTG 2 cut(s) 428, 531
BveI ACCTGC 1 cut(s) 268
CfoI GCGC 1 cut(s) 96
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 4 cut(s) 157, 205, 345, 372
Cfr42I CCGCGG 1 cut(s) 211
CpoI CGGWCCG 1 cut(s) 205
CseI GACGC 1 cut(s) 143
CspI CGGWCCG 1 cut(s) 205
CviAII CATG 2 cut(s) 19, 331
DdeI CTNAG 2 cut(s) 624, 644
DpnI GATC 1 cut(s) 222
DpnII GATC 1 cut(s) 220
DriI GACNNNNNGTC 1 cut(s) 163
Eam1104I CTCTTC 2 cut(s) 296, 305
Eam1105I GACNNNNNGTC 1 cut(s) 163
EarI CTCTTC 2 cut(s) 296, 305
EciI GGCGGA 1 cut(s) 181
Eco130I CCWWGG 1 cut(s) 18
Eco24I GRGCYC 2 cut(s) 147, 645
Eco31I GGTCTC 2 cut(s) 374, 512
Eco47I GGWCC 3 cut(s) 157, 205, 345
Eco57I CTGAAG 1 cut(s) 329
Eco88I CYCGRG 1 cut(s) 230
Eco91I GGTNACC 1 cut(s) 325
EcoO109I RGGNCCY 1 cut(s) 372
EcoO65I GGTNACC 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 484
EcoT14I CCWWGG 1 cut(s) 18
EcoT38I GRGCYC 2 cut(s) 147, 645
ErhI CCWWGG 1 cut(s) 18
FaeI CATG 2 cut(s) 22, 334
FaiI YATR 5 cut(s) 20, 258, 332, 402, 499
FaqI GGGAC 1 cut(s) 358
FatI CATG 2 cut(s) 18, 330
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 2 cut(s) 227, 352
FriOI GRGCYC 2 cut(s) 147, 645
Fsp4HI GCNGC 2 cut(s) 227, 352
GlaI GCGC 1 cut(s) 95
GluI GCNGC 2 cut(s) 227, 352
GsaI CCCAGC 1 cut(s) 78
GsuI CTGGAG 1 cut(s) 536
HaeIII GGCC 1 cut(s) 373
HapII CCGG 4 cut(s) 203, 348, 367, 446
HgaI GACGC 1 cut(s) 143
HhaI GCGC 1 cut(s) 96
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 2 cut(s) 22, 334
Hin6I GCGC 1 cut(s) 94
HinP1I GCGC 1 cut(s) 94
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HinfI GANTC 3 cut(s) 164, 240, 289
HpaII CCGG 4 cut(s) 203, 348, 367, 446
HphI GGTGA 2 cut(s) 142, 337
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 5 cut(s) 133, 157, 169, 647, 665
Hpy188III TCNNGA 3 cut(s) 232, 446, 515
Hpy8I GTNNAC 1 cut(s) 244
Hpy99I CGWCG 5 cut(s) 137, 248, 251, 397, 561
HpyAV CCTTC 2 cut(s) 198, 500
HpyCH4III ACNGT 2 cut(s) 82, 634
HpyCH4IV ACGT 2 cut(s) 123, 246
HpyCH4V TGCA 3 cut(s) 102, 298, 472
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 283
HpyF3I CTNAG 2 cut(s) 624, 644
HpySE526I ACGT 2 cut(s) 123, 246
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 2 cut(s) 22, 334
HspAI GCGC 1 cut(s) 94
Kpn2I TCCGGA 1 cut(s) 445
KspI CCGCGG 1 cut(s) 211
Kzo9I GATC 1 cut(s) 220
LweI GCATC 1 cut(s) 610
MaeII ACGT 2 cut(s) 123, 246
MaeIII GTNAC 3 cut(s) 124, 325, 634
MalI GATC 1 cut(s) 222
MbiI CCGCTC 1 cut(s) 229
MboI GATC 1 cut(s) 220
MboII GAAGA 3 cut(s) 313, 322, 520
MfeI CAATTG 1 cut(s) 339
MhlI GDGCHC 4 cut(s) 87, 147, 322, 645
MluCI AATT 2 cut(s) 339, 492
MlyI GAGTC 3 cut(s) 173, 249, 298
MmeI TCCRAC 2 cut(s) 156, 192
MnlI CCTC 9 cut(s) 19, 22, 25, 56, 139, 163, 363, 444, 641
MroI TCCGGA 1 cut(s) 445
MspA1I CMGCKG 2 cut(s) 210, 411
MspI CCGG 4 cut(s) 203, 348, 367, 446
MspR9I CCNGG 2 cut(s) 203, 486
MunI CAATTG 1 cut(s) 339
Mva1269I GAATGC 2 cut(s) 354, 481
MvaI CCWGG 1 cut(s) 486
MvnI CGCG 2 cut(s) 94, 210
MwoI GCNNNNNNNGC 2 cut(s) 91, 283
NciI CCSGG 1 cut(s) 203
NcoI CCATGG 1 cut(s) 18
NdeII GATC 1 cut(s) 220
NlaIII CATG 2 cut(s) 22, 334
NlaIV GGNNCC 1 cut(s) 346
NmeAIII GCCGAG 1 cut(s) 435
NmuCI GTSAC 1 cut(s) 325
NspV TTCGAA 1 cut(s) 358
PaeR7I CTCGAG 1 cut(s) 230
PaqCI CACCTGC 1 cut(s) 268
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 243
PctI GAATGC 2 cut(s) 354, 481
PflMI CCANNNNNTGG 1 cut(s) 545
PkrI GCNGC 2 cut(s) 228, 353
PleI GAGTC 3 cut(s) 172, 248, 297
PpsI GAGTC 3 cut(s) 172, 248, 297
PsiI TTATAA 1 cut(s) 258
Psp6I CCWGG 1 cut(s) 484
PspEI GGTNACC 1 cut(s) 325
PspFI CCCAGC 1 cut(s) 74
PspGI CCWGG 1 cut(s) 484
PspN4I GGNNCC 1 cut(s) 346
PspPI GGNCC 4 cut(s) 157, 205, 345, 372
Rsr2I CGGWCCG 1 cut(s) 205
RsrII CGGWCCG 1 cut(s) 205
SacII CCGCGG 1 cut(s) 211
SalI GTCGAC 1 cut(s) 242
SatI GCNGC 2 cut(s) 227, 352
Sau3AI GATC 1 cut(s) 220
Sau96I GGNCC 4 cut(s) 157, 205, 345, 372
SchI GAGTC 3 cut(s) 173, 249, 298
ScrFI CCNGG 2 cut(s) 203, 486
SduI GDGCHC 4 cut(s) 87, 147, 322, 645
SetI ASST 9 cut(s) 126, 131, 155, 162, 249, 282, 327, 511, 652
SfaNI GCATC 1 cut(s) 610
Sfr274I CTCGAG 1 cut(s) 230
Sfr303I CCGCGG 1 cut(s) 211
SfuI TTCGAA 1 cut(s) 358
SgrBI CCGCGG 1 cut(s) 211
SinI GGWCC 3 cut(s) 157, 205, 345
SlaI CTCGAG 1 cut(s) 230
SmlI CTYRAG 3 cut(s) 31, 161, 230
SmoI CTYRAG 3 cut(s) 31, 161, 230
Sse9I AATT 2 cut(s) 339, 492
SsiI CCGC 7 cut(s) 192, 208, 210, 227, 351, 409, 467
StyD4I CCNGG 2 cut(s) 201, 484
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 2 cut(s) 82, 634
TaiI ACGT 2 cut(s) 126, 249
TaqI TCGA 4 cut(s) 231, 243, 358, 556
TaqII GACCGA 1 cut(s) 398
TasI AATT 2 cut(s) 339, 492
TauI GCSGC 2 cut(s) 229, 354
TscAI CASTG 2 cut(s) 428, 538
TseFI GTSAC 1 cut(s) 325
Tsp45I GTSAC 1 cut(s) 325
TspGWI ACGGA 3 cut(s) 252, 282, 410
TspRI CASTG 2 cut(s) 428, 538
Van91I CCANNNNNTGG 1 cut(s) 545
VpaK11BI GGWCC 3 cut(s) 157, 205, 345
XcmI CCANNNNNNNNNTGG 1 cut(s) 412
XhoI CTCGAG 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 243
ZraI GACGTC 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.