Prupe.5G215900_v2.0.a1

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0014454
Physical Location & Seq
Reverse (-)
16957487 .. 16959457
1971 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G215900.1

Sequence Viewer

Length: 696 bp
ATGTCTACCTCCTCCTCCCTGGCTACGCTTTCCAGGAGACTCTGTCGCTCTCTGCACTCAAATCCTATGAATGTACTCTCTATGCGCTTCTGCACCACCACGACCACCGACCTCTCCTCCTCCTCTAGCGACAGCTCTGACTCAGACGAGTCCAACCCTCTCAACTCGGAGCCCAACTCGCCCCAAGACAACGTCCCCCCACCACAGCCGCCACCAGAGGACTCCAATCGGAAAAACTTTTTCAACGATCGCCCGCTCGAGAACGGCCTCGACGTAGGCATTTACAAGGCGATACTGGTGGGGCTGGTGGGGCAGAGTCCGTTGCAGAAGAAGCTGAAGAGCGGGGCCACGGTCACCATGTTTTCTGTTGGGACCGGCGGCATTCGGAACAACCGGAGGCCGTTCAATAACGAGGACCCGTCGGAGTACGCAAATCGCTGTGCCATTCAGTGGCACCGCGTTTGCATTTATCCAGATAATCTTGGCATGATCGCCATGAAGAATGCTTTGCCTGGTTCACGTTTATACTTGGAAGGTAATTTGGAGACCAAAATATTCACTGACCCGATAAGTGGGCTTGTTCGACGTGTTAGAGAGATTGCTGTTCGTAGAAATGGTCGCCTTGTGTTTTTGGGTAAAGGTGATGGTGCTGAACAACCGCCGAAATGGGGGCTGAAAAGTGTTGGCTACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03430 ko03440 map03030 map03430 map03440
Pfam Domains
Protein Families
SSB

Protein Analysis

232

Amino Acids

25.45

Weight (kDa)

9.25

Isoelectric Point (pI)

57.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014454)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18580
fragaria_vesca FvH4_5g12820 FvH4_5g12820
malus_domestica MD06G1209800.v1.1 MD14G1220600.v1.1
prunus_persica Prupe.5G215900_v2.0.a1
pyrus_communis pycom06g18740
rosa_chinensis RchiOBHm_Chr7g0184411
rosa_laevigata RLG00000004977
rosa_multiflora Rmu_co8454851.1_g000001 Rmu_sc0001557.1_g000018
rosa_roxburghii Rroxscaffold_3G00269650
rosa_samantha Rh7AG080200 Rh7BG067000 Rh7CG067300 Rh7DG067000
rosa_wichuraiana Rw7G005510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 453
AccB7I CCANNNNNTGG 1 cut(s) 450
AccBSI CCGCTC 2 cut(s) 256, 342
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 459
AciI CCGC 6 cut(s) 209, 254, 342, 378, 457, 659
AcuI CTGAAG 1 cut(s) 356
AfaI GTAC 2 cut(s) 75, 428
AfiI CCNNNNNNNGG 3 cut(s) 450, 572, 668
AflIII ACRYGT 1 cut(s) 586
AgsI TTSAA 2 cut(s) 244, 406
AjiI CACGTC 1 cut(s) 587
AjnI CCWGG 3 cut(s) 18, 32, 511
AluBI AGCT 2 cut(s) 135, 334
AluI AGCT 2 cut(s) 135, 334
Alw26I GTCTC 2 cut(s) 31, 539
Ama87I CYCGRG 1 cut(s) 257
AoxI GGCC 3 cut(s) 265, 345, 398
AspLEI GCGC 1 cut(s) 87
AspS9I GGNCC 3 cut(s) 345, 372, 415
AsuHPI GGTGA 2 cut(s) 346, 653
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 2 cut(s) 372, 415
BanI GGYRCC 1 cut(s) 453
BanII GRGCYC 1 cut(s) 174
BccI CCATC 1 cut(s) 638
BceAI ACGGC 2 cut(s) 280, 385
BciT130I CCWGG 3 cut(s) 20, 34, 513
BcoDI GTCTC 2 cut(s) 31, 539
BfaI CTAG 1 cut(s) 126
BisI GCNGC 2 cut(s) 209, 379
BlsI GCNGC 2 cut(s) 210, 380
Bme1390I CCNGG 3 cut(s) 20, 34, 513
Bme18I GGWCC 2 cut(s) 372, 415
BmeT110I CYCGRG 1 cut(s) 257
BmgBI CACGTC 1 cut(s) 587
BmgT120I GGNCC 3 cut(s) 345, 372, 415
BmiI GGNNCC 5 cut(s) 171, 346, 373, 417, 455
BmrFI CCNGG 3 cut(s) 20, 34, 513
BplI GAGNNNNNCTC 2 cut(s) 161, 193
BsaI GGTCTC 1 cut(s) 539
BsaJI CCNNGG 2 cut(s) 18, 348
BsaWI WCCGGW 1 cut(s) 393
BsaXI ACNNNNNCTCC 4 cut(s) 28, 58, 101, 131
Bsc4I CCNNNNNNNGG 3 cut(s) 450, 572, 668
Bse118I RCCGGY 1 cut(s) 374
Bse1I ACTGG 1 cut(s) 300
BseBI CCWGG 3 cut(s) 20, 34, 513
BseDI CCNNGG 2 cut(s) 18, 348
BseLI CCNNNNNNNGG 3 cut(s) 450, 572, 668
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 1 cut(s) 300
BseRI GAGGAG 4 cut(s) 4, 106, 109, 112
BsgI GTGCAG 2 cut(s) 38, 76
Bsh1236I CGCG 1 cut(s) 459
Bsh1285I CGRYCG 1 cut(s) 250
BshFI GGCC 3 cut(s) 267, 347, 400
BshNI GGYRCC 1 cut(s) 453
BsiEI CGRYCG 1 cut(s) 250
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 2 cut(s) 375, 394
BslFI GGGAC 2 cut(s) 179, 385
BslI CCNNNNNNNGG 3 cut(s) 450, 572, 668
BsmAI GTCTC 2 cut(s) 31, 539
BsmFI GGGAC 2 cut(s) 179, 385
BsmI GAATGC 2 cut(s) 381, 508
BsnI GGCC 3 cut(s) 267, 347, 400
Bso31I GGTCTC 1 cut(s) 539
BsoBI CYCGRG 1 cut(s) 257
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 2 cut(s) 247, 489
BspACI CCGC 6 cut(s) 209, 254, 342, 378, 457, 659
BspANI GGCC 3 cut(s) 267, 347, 400
BspCNI CTCAG 1 cut(s) 155
BspFNI CGCG 1 cut(s) 459
BspLI GGNNCC 5 cut(s) 171, 346, 373, 417, 455
BspQI GCTCTTC 1 cut(s) 332
BspT107I GGYRCC 1 cut(s) 453
BspTNI GGTCTC 1 cut(s) 539
BsrBI CCGCTC 2 cut(s) 256, 342
BsrFI RCCGGY 1 cut(s) 374
BsrI ACTGG 1 cut(s) 300
BssAI RCCGGY 1 cut(s) 374
BssECI CCNNGG 2 cut(s) 18, 348
BssMI GATC 2 cut(s) 247, 489
Bst2UI CCWGG 3 cut(s) 20, 34, 513
Bst4CI ACNGT 1 cut(s) 352
Bst6I CTCTTC 1 cut(s) 332
BstC8I GCNNGC 1 cut(s) 254
BstDEI CTNAG 1 cut(s) 142
BstDSI CCRYGG 1 cut(s) 348
BstEII GGTNACC 1 cut(s) 352
BstFNI CGCG 1 cut(s) 459
BstHHI GCGC 1 cut(s) 87
BstKTI GATC 2 cut(s) 250, 492
BstMAI GTCTC 2 cut(s) 31, 539
BstMBI GATC 2 cut(s) 247, 489
BstMCI CGRYCG 1 cut(s) 250
BstMWI GCNNNNNNNGC 3 cut(s) 178, 310, 331
BstNI CCWGG 3 cut(s) 20, 34, 513
BstPI GGTNACC 1 cut(s) 352
BstSCI CCNGG 3 cut(s) 18, 32, 511
BstUI CGCG 1 cut(s) 459
BsuRI GGCC 3 cut(s) 267, 347, 400
BtgI CCRYGG 1 cut(s) 348
BtrI CACGTC 1 cut(s) 587
BtsIMutI CAGTG 2 cut(s) 455, 558
Cac8I GCNNGC 1 cut(s) 254
CfoI GCGC 1 cut(s) 87
Cfr10I RCCGGY 1 cut(s) 374
Cfr13I GGNCC 3 cut(s) 345, 372, 415
Csp6I GTAC 2 cut(s) 74, 427
CviAII CATG 3 cut(s) 358, 487, 496
CviQI GTAC 2 cut(s) 74, 427
DdeI CTNAG 1 cut(s) 142
DpnI GATC 2 cut(s) 249, 491
DpnII GATC 2 cut(s) 247, 489
Eam1104I CTCTTC 1 cut(s) 332
EarI CTCTTC 1 cut(s) 332
Eco24I GRGCYC 1 cut(s) 174
Eco31I GGTCTC 1 cut(s) 539
Eco47I GGWCC 2 cut(s) 372, 415
Eco57I CTGAAG 1 cut(s) 356
Eco88I CYCGRG 1 cut(s) 257
Eco91I GGTNACC 1 cut(s) 352
EcoO109I RGGNCCY 1 cut(s) 415
EcoO65I GGTNACC 1 cut(s) 352
EcoRII CCWGG 3 cut(s) 18, 32, 511
EcoT38I GRGCYC 1 cut(s) 174
FaeI CATG 3 cut(s) 361, 490, 499
FaiI YATR 6 cut(s) 68, 83, 359, 488, 497, 526
FaqI GGGAC 2 cut(s) 179, 385
FatI CATG 3 cut(s) 357, 486, 495
FauI CCCGC 2 cut(s) 261, 335
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 2 cut(s) 209, 379
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 2 cut(s) 209, 379
FspBI CTAG 1 cut(s) 126
GlaI GCGC 1 cut(s) 86
GluI GCNGC 2 cut(s) 209, 379
HaeIII GGCC 3 cut(s) 267, 347, 400
HapII CCGG 2 cut(s) 375, 394
HhaI GCGC 1 cut(s) 87
Hin1II CATG 3 cut(s) 361, 490, 499
Hin6I GCGC 1 cut(s) 85
HinP1I GCGC 1 cut(s) 85
HinfI GANTC 5 cut(s) 39, 140, 149, 221, 316
HpaII CCGG 2 cut(s) 375, 394
HphI GGTGA 2 cut(s) 346, 653
Hpy166II GTNNAC 2 cut(s) 6, 518
Hpy188I TCNGA 6 cut(s) 139, 145, 169, 231, 387, 424
Hpy188III TCNNGA 2 cut(s) 259, 473
Hpy8I GTNNAC 2 cut(s) 6, 518
Hpy99I CGWCG 3 cut(s) 275, 424, 588
HpyAV CCTTC 1 cut(s) 527
HpyCH4III ACNGT 1 cut(s) 352
HpyCH4IV ACGT 4 cut(s) 192, 273, 520, 586
HpyCH4V TGCA 4 cut(s) 55, 93, 325, 465
HpyF10VI GCNNNNNNNGC 3 cut(s) 178, 310, 331
HpyF3I CTNAG 1 cut(s) 142
HpySE526I ACGT 4 cut(s) 192, 273, 520, 586
Hsp92II CATG 3 cut(s) 361, 490, 499
HspAI GCGC 1 cut(s) 85
Kzo9I GATC 2 cut(s) 247, 489
LguI GCTCTTC 1 cut(s) 332
LmnI GCTCC 1 cut(s) 169
MaeI CTAG 1 cut(s) 126
MaeII ACGT 4 cut(s) 192, 273, 520, 586
MaeIII GTNAC 1 cut(s) 352
MalI GATC 2 cut(s) 249, 491
MbiI CCGCTC 2 cut(s) 256, 342
MboI GATC 2 cut(s) 247, 489
MboII GAAGA 3 cut(s) 340, 349, 511
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 1 cut(s) 538
MlyI GAGTC 5 cut(s) 33, 134, 158, 215, 325
MmeI TCCRAC 2 cut(s) 177, 402
MspI CCGG 2 cut(s) 375, 394
MspR9I CCNGG 3 cut(s) 20, 34, 513
Mva1269I GAATGC 2 cut(s) 381, 508
MvaI CCWGG 3 cut(s) 20, 34, 513
MvnI CGCG 1 cut(s) 459
MwoI GCNNNNNNNGC 3 cut(s) 178, 310, 331
NdeII GATC 2 cut(s) 247, 489
NlaIII CATG 3 cut(s) 361, 490, 499
NlaIV GGNNCC 5 cut(s) 171, 346, 373, 417, 455
NmuCI GTSAC 1 cut(s) 352
PaeR7I CTCGAG 1 cut(s) 257
PciSI GCTCTTC 1 cut(s) 332
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PctI GAATGC 2 cut(s) 381, 508
PflFI GACNNNGTC 2 cut(s) 42, 191
PflMI CCANNNNNTGG 1 cut(s) 450
PfoI TCCNGGA 1 cut(s) 32
PkrI GCNGC 2 cut(s) 210, 380
Ple19I CGATCG 1 cut(s) 250
PleI GAGTC 5 cut(s) 33, 134, 157, 215, 324
PpsI GAGTC 5 cut(s) 33, 134, 157, 215, 324
PpuMI RGGWCCY 1 cut(s) 415
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 3 cut(s) 18, 32, 511
PspEI GGTNACC 1 cut(s) 352
PspGI CCWGG 3 cut(s) 18, 32, 511
PspN4I GGNNCC 5 cut(s) 171, 346, 373, 417, 455
PspPI GGNCC 3 cut(s) 345, 372, 415
PspPPI RGGWCCY 1 cut(s) 415
PsyI GACNNNGTC 2 cut(s) 42, 191
PvuI CGATCG 1 cut(s) 250
RsaI GTAC 2 cut(s) 75, 428
RsaNI GTAC 2 cut(s) 74, 427
SapI GCTCTTC 1 cut(s) 332
SatI GCNGC 2 cut(s) 209, 379
Sau3AI GATC 2 cut(s) 247, 489
Sau96I GGNCC 3 cut(s) 345, 372, 415
SchI GAGTC 5 cut(s) 33, 134, 158, 215, 325
ScrFI CCNGG 3 cut(s) 20, 34, 513
SduI GDGCHC 1 cut(s) 174
Sfr274I CTCGAG 1 cut(s) 257
SinI GGWCC 2 cut(s) 372, 415
SlaI CTCGAG 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 1 cut(s) 538
SsiI CCGC 6 cut(s) 209, 254, 342, 378, 457, 659
SspI AATATT 1 cut(s) 555
SspMI CTAG 1 cut(s) 126
StyD4I CCNGG 3 cut(s) 18, 32, 511
TaaI ACNGT 1 cut(s) 352
TaiI ACGT 4 cut(s) 195, 276, 523, 589
TaqI TCGA 3 cut(s) 258, 270, 583
TasI AATT 1 cut(s) 538
TatI WGTACW 1 cut(s) 73
TauI GCSGC 2 cut(s) 211, 381
TscAI CASTG 2 cut(s) 455, 565
TseFI GTSAC 1 cut(s) 352
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 2 cut(s) 83, 512
TspGWI ACGGA 1 cut(s) 309
TspRI CASTG 2 cut(s) 455, 565
Tth111I GACNNNGTC 2 cut(s) 42, 191
Van91I CCANNNNNTGG 1 cut(s) 450
VpaK11BI GGWCC 2 cut(s) 372, 415
XhoI CTCGAG 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.