RchiOBHm_Chr7g0184411

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0014454
Physical Location & Seq
Reverse (-)
4966191 .. 4968283
2093 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16453

Sequence Viewer

Length: 825 bp
ATGATTGGCTCTCTCACTTGCAAATCCAAACAGAGGCCCAACAATAATGGGCTCGGGTCAAAATTTCAGACCCCAAAACCCATTTCTCACCACCACCAAGGGTTTAAGCTCCCCAAAACCTCCACAAAGCTAGAGCTCCATTTTTGCTGCCTGAAAATGTCAGCCTCCTCCTCCATGGCTTCACTCTCAAGGAGACTACTCCTCTCTCGCTCTCTCTACCACTCAAACCCCAGCACCGTGCTCTCCACGCGCTTCTGCACCACCACCACCACCACCCCCCACGTCACCTCCGACTCAGACGAGCCCATCACCTCGGACCTAGAGTCCGACCCGACCCCATCACAGCCCTCCGCCCCCAACCCGGTCCGCGGTATCTACGATCAGCCGCTCGAGAACGGAGTCGACGTCGGCATTTATAAGGCGATACTGGTGGGGCAGGTGGGGCAGAGTCCGTTGCAGAAGAGACTGAAGAGCGGGGCGCAGGTGACCATGTTCTCAATTGGGACCGGCGGCATTCGAAACAACCGGAGGCCCTTTGAGACCGAGAACCCGACGGAGTATGCCAACCGCTGTGTGGTTCAGTGGCACAGGGTTTGTGTTTATCCGGAGAGGCTCGGCAAGATTGCGGTGCAGAATGCTTTGCCTGGTTCAATTTTATACTTGGAAGGTAATCTGGAGACCAAAATCTTCACTGACCCAACAACTGGGCTTGTTCGACGAGTTAGAGAGATTGCTATTCGCAGAAATGGTCGCCTTGTGTTTTTGGGTAAAGGAAACGATGCTGAGCCAACAGTAACGGGGCTCAGAGGTGTTGGCTACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03430 ko03440 map03030 map03430 map03440
Pfam Domains
Protein Families
SSB

Protein Analysis

274

Amino Acids

30.12

Weight (kDa)

9.77

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 140 - 247 9.1e-14 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014454)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18580
fragaria_vesca FvH4_5g12820 FvH4_5g12820
malus_domestica MD06G1209800.v1.1 MD14G1220600.v1.1
prunus_persica Prupe.5G215900_v2.0.a1
pyrus_communis pycom06g18740
rosa_chinensis RchiOBHm_Chr7g0184411
rosa_laevigata RLG00000004977
rosa_multiflora Rmu_co8454851.1_g000001 Rmu_sc0001557.1_g000018
rosa_roxburghii Rroxscaffold_3G00269650
rosa_samantha Rh7AG080200 Rh7BG067000 Rh7CG067300 Rh7DG067000
rosa_wichuraiana Rw7G005510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 417
AarI CACCTGC 2 cut(s) 427, 472
AatII GACGTC 1 cut(s) 408
Acc36I ACCTGC 2 cut(s) 427, 472
AccB7I CCANNNNNTGG 1 cut(s) 704
AccBSI CCGCTC 2 cut(s) 388, 474
AccI GTMKAC 1 cut(s) 402
AccII CGCG 2 cut(s) 250, 369
AccIII TCCGGA 1 cut(s) 604
AciI CCGC 8 cut(s) 351, 367, 369, 386, 474, 510, 568, 626
AcsI RAATTY 1 cut(s) 62
AcuI CTGAAG 1 cut(s) 488
AcyI GRCGYC 1 cut(s) 405
AfiI CCNNNNNNNGG 5 cut(s) 33, 361, 368, 574, 704
AgsI TTSAA 1 cut(s) 651
AhdI GACNNNNNGTC 1 cut(s) 322
AjiI CACGTC 1 cut(s) 283
AjnI CCWGG 1 cut(s) 643
AjuI GAANNNNNNNTTGG 2 cut(s) 67, 99
AluBI AGCT 3 cut(s) 109, 130, 136
AluI AGCT 3 cut(s) 109, 130, 136
Alw21I GWGCWC 2 cut(s) 138, 243
Alw26I GTCTC 4 cut(s) 187, 457, 533, 671
Ama87I CYCGRG 2 cut(s) 53, 389
Aor13HI TCCGGA 1 cut(s) 604
AoxI GGCC 2 cut(s) 35, 530
ApeKI GCWGC 1 cut(s) 147
ApoI RAATTY 1 cut(s) 62
AspLEI GCGC 2 cut(s) 252, 481
AspS9I GGNCC 5 cut(s) 36, 316, 364, 504, 531
AsuC2I CCSGG 1 cut(s) 362
AsuHPI GGTGA 4 cut(s) 80, 277, 301, 496
AsuII TTCGAA 1 cut(s) 517
AvaI CYCGRG 2 cut(s) 53, 389
AvaII GGWCC 3 cut(s) 316, 364, 504
BanII GRGCYC 4 cut(s) 54, 138, 306, 804
Bbv12I GWGCWC 2 cut(s) 138, 243
BbvI GCAGC 1 cut(s) 134
BccI CCATC 2 cut(s) 314, 346
BciT130I CCWGG 1 cut(s) 645
BcnI CCSGG 1 cut(s) 362
BcoDI GTCTC 4 cut(s) 187, 457, 533, 671
BfaI CTAG 2 cut(s) 131, 320
BfuAI ACCTGC 2 cut(s) 427, 472
BisI GCNGC 3 cut(s) 148, 386, 511
BlpI GCTNAGC 1 cut(s) 783
BlsI GCNGC 3 cut(s) 149, 387, 512
Bme1390I CCNGG 2 cut(s) 362, 645
Bme18I GGWCC 3 cut(s) 316, 364, 504
BmeRI GACNNNNNGTC 1 cut(s) 322
BmeT110I CYCGRG 2 cut(s) 53, 389
BmgBI CACGTC 1 cut(s) 283
BmgT120I GGNCC 5 cut(s) 36, 316, 364, 504, 531
BmiI GGNNCC 1 cut(s) 505
BmrFI CCNGG 2 cut(s) 362, 645
BmrI ACTGGG 1 cut(s) 714
BmsI GCATC 1 cut(s) 769
BmuI ACTGGG 1 cut(s) 714
BpmI CTGGAG 1 cut(s) 695
Bpu1102I GCTNAGC 1 cut(s) 783
Bpu14I TTCGAA 1 cut(s) 517
BpuEI CTTGAG 1 cut(s) 172
BpuMI CCSGG 1 cut(s) 362
BsaHI GRCGYC 1 cut(s) 405
BsaI GGTCTC 2 cut(s) 533, 671
BsaJI CCNNGG 4 cut(s) 97, 174, 312, 367
BsaWI WCCGGW 2 cut(s) 525, 604
BsaXI ACNNNNNCTCC 4 cut(s) 272, 302, 390, 420
Bsc4I CCNNNNNNNGG 5 cut(s) 33, 361, 368, 574, 704
Bse118I RCCGGY 1 cut(s) 506
Bse1I ACTGG 2 cut(s) 432, 709
BseAI TCCGGA 1 cut(s) 604
BseBI CCWGG 1 cut(s) 645
BseDI CCNNGG 4 cut(s) 97, 174, 312, 367
BseLI CCNNNNNNNGG 5 cut(s) 33, 361, 368, 574, 704
BseMII CTCAG 3 cut(s) 309, 774, 817
BseNI ACTGG 2 cut(s) 432, 709
BseRI GAGGAG 3 cut(s) 157, 160, 191
BseXI GCAGC 1 cut(s) 134
BseYI CCCAGC 1 cut(s) 230
BsgI GTGCAG 2 cut(s) 241, 650
Bsh1236I CGCG 2 cut(s) 250, 369
BshFI GGCC 2 cut(s) 37, 532
BsiHKAI GWGCWC 2 cut(s) 138, 243
BsiHKCI CYCGRG 2 cut(s) 53, 389
BsiSI CCGG 4 cut(s) 362, 507, 526, 605
BslFI GGGAC 1 cut(s) 517
BslI CCNNNNNNNGG 5 cut(s) 33, 361, 368, 574, 704
BsmAI GTCTC 4 cut(s) 187, 457, 533, 671
BsmFI GGGAC 1 cut(s) 517
BsmI GAATGC 2 cut(s) 513, 640
BsnI GGCC 2 cut(s) 37, 532
Bso31I GGTCTC 2 cut(s) 533, 671
BsoBI CYCGRG 2 cut(s) 53, 389
Bsp119I TTCGAA 1 cut(s) 517
Bsp1286I GDGCHC 5 cut(s) 54, 138, 243, 306, 804
Bsp13I TCCGGA 1 cut(s) 604
Bsp143I GATC 1 cut(s) 379
Bsp1720I GCTNAGC 1 cut(s) 783
Bsp19I CCATGG 1 cut(s) 174
BspACI CCGC 8 cut(s) 351, 367, 369, 386, 474, 510, 568, 626
BspANI GGCC 2 cut(s) 37, 532
BspCNI CTCAG 3 cut(s) 308, 775, 816
BspEI TCCGGA 1 cut(s) 604
BspFNI CGCG 2 cut(s) 250, 369
BspLI GGNNCC 1 cut(s) 505
BspMI ACCTGC 2 cut(s) 427, 472
BspQI GCTCTTC 1 cut(s) 464
BspT104I TTCGAA 1 cut(s) 517
BspTNI GGTCTC 2 cut(s) 533, 671
BsrBI CCGCTC 2 cut(s) 388, 474
BsrFI RCCGGY 1 cut(s) 506
BsrI ACTGG 2 cut(s) 432, 709
BssAI RCCGGY 1 cut(s) 506
BssECI CCNNGG 4 cut(s) 97, 174, 312, 367
BssMI GATC 1 cut(s) 379
BssNI GRCGYC 1 cut(s) 405
BssT1I CCWWGG 2 cut(s) 97, 174
Bst2UI CCWGG 1 cut(s) 645
Bst4CI ACNGT 2 cut(s) 238, 793
Bst6I CTCTTC 2 cut(s) 455, 464
BstACI GRCGYC 1 cut(s) 405
BstBI TTCGAA 1 cut(s) 517
BstDEI CTNAG 3 cut(s) 295, 783, 803
BstDSI CCRYGG 2 cut(s) 174, 367
BstEII GGTNACC 1 cut(s) 484
BstFNI CGCG 2 cut(s) 250, 369
BstHHI GCGC 2 cut(s) 252, 481
BstKTI GATC 1 cut(s) 382
BstMAI GTCTC 4 cut(s) 187, 457, 533, 671
BstMBI GATC 1 cut(s) 379
BstMWI GCNNNNNNNGC 2 cut(s) 247, 442
BstNI CCWGG 1 cut(s) 645
BstPI GGTNACC 1 cut(s) 484
BstSCI CCNGG 2 cut(s) 360, 643
BstUI CGCG 2 cut(s) 250, 369
BstV1I GCAGC 1 cut(s) 134
BsuRI GGCC 2 cut(s) 37, 532
BtgI CCRYGG 2 cut(s) 174, 367
BtrI CACGTC 1 cut(s) 283
BtsIMutI CAGTG 2 cut(s) 587, 690
BveI ACCTGC 2 cut(s) 427, 472
CfoI GCGC 2 cut(s) 252, 481
Cfr10I RCCGGY 1 cut(s) 506
Cfr13I GGNCC 5 cut(s) 36, 316, 364, 504, 531
Cfr42I CCGCGG 1 cut(s) 370
CpoI CGGWCCG 1 cut(s) 364
CspI CGGWCCG 1 cut(s) 364
CviAII CATG 2 cut(s) 175, 490
DdeI CTNAG 3 cut(s) 295, 783, 803
DpnI GATC 1 cut(s) 381
DpnII GATC 1 cut(s) 379
DriI GACNNNNNGTC 1 cut(s) 322
Eam1104I CTCTTC 2 cut(s) 455, 464
Eam1105I GACNNNNNGTC 1 cut(s) 322
EarI CTCTTC 2 cut(s) 455, 464
EciI GGCGGA 1 cut(s) 340
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 2 cut(s) 97, 174
Eco24I GRGCYC 4 cut(s) 54, 138, 306, 804
Eco31I GGTCTC 2 cut(s) 533, 671
Eco47I GGWCC 3 cut(s) 316, 364, 504
Eco53kI GAGCTC 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 488
Eco88I CYCGRG 2 cut(s) 53, 389
Eco91I GGTNACC 1 cut(s) 484
EcoICRI GAGCTC 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 531
EcoO65I GGTNACC 1 cut(s) 484
EcoRII CCWGG 1 cut(s) 643
EcoT14I CCWWGG 2 cut(s) 97, 174
EcoT38I GRGCYC 4 cut(s) 54, 138, 306, 804
ErhI CCWWGG 2 cut(s) 97, 174
FaeI CATG 2 cut(s) 178, 493
FaiI YATR 5 cut(s) 176, 417, 491, 561, 658
FaqI GGGAC 1 cut(s) 517
FatI CATG 2 cut(s) 174, 489
FauI CCCGC 1 cut(s) 467
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 3 cut(s) 148, 386, 511
FriOI GRGCYC 4 cut(s) 54, 138, 306, 804
Fsp4HI GCNGC 3 cut(s) 148, 386, 511
FspBI CTAG 2 cut(s) 131, 320
GlaI GCGC 2 cut(s) 251, 480
GluI GCNGC 3 cut(s) 148, 386, 511
GsaI CCCAGC 1 cut(s) 234
GsuI CTGGAG 1 cut(s) 695
HaeIII GGCC 2 cut(s) 37, 532
HapII CCGG 4 cut(s) 362, 507, 526, 605
HhaI GCGC 2 cut(s) 252, 481
Hin1I GRCGYC 1 cut(s) 405
Hin1II CATG 2 cut(s) 178, 493
Hin6I GCGC 2 cut(s) 250, 479
HinP1I GCGC 2 cut(s) 250, 479
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HinfI GANTC 4 cut(s) 293, 323, 399, 448
HpaII CCGG 4 cut(s) 362, 507, 526, 605
HphI GGTGA 4 cut(s) 80, 277, 301, 496
Hpy166II GTNNAC 1 cut(s) 403
Hpy188I TCNGA 7 cut(s) 69, 292, 298, 316, 328, 806, 824
Hpy188III TCNNGA 3 cut(s) 391, 605, 674
Hpy8I GTNNAC 1 cut(s) 403
Hpy99I CGWCG 4 cut(s) 407, 410, 556, 720
HpyAV CCTTC 1 cut(s) 659
HpyCH4III ACNGT 2 cut(s) 238, 793
HpyCH4IV ACGT 2 cut(s) 282, 405
HpyCH4V TGCA 4 cut(s) 21, 258, 457, 631
HpyF10VI GCNNNNNNNGC 2 cut(s) 247, 442
HpyF3I CTNAG 3 cut(s) 295, 783, 803
HpySE526I ACGT 2 cut(s) 282, 405
Hsp92I GRCGYC 1 cut(s) 405
Hsp92II CATG 2 cut(s) 178, 493
HspAI GCGC 2 cut(s) 250, 479
Kpn2I TCCGGA 1 cut(s) 604
KspI CCGCGG 1 cut(s) 370
Kzo9I GATC 1 cut(s) 379
LguI GCTCTTC 1 cut(s) 464
LmnI GCTCC 2 cut(s) 114, 141
Lsp1109I GCAGC 1 cut(s) 134
LweI GCATC 1 cut(s) 769
MaeI CTAG 2 cut(s) 131, 320
MaeII ACGT 2 cut(s) 282, 405
MaeIII GTNAC 3 cut(s) 283, 484, 793
MalI GATC 1 cut(s) 381
MbiI CCGCTC 2 cut(s) 388, 474
MboI GATC 1 cut(s) 379
MboII GAAGA 3 cut(s) 472, 481, 679
MfeI CAATTG 1 cut(s) 498
MhlI GDGCHC 5 cut(s) 54, 138, 243, 306, 804
MluCI AATT 3 cut(s) 62, 498, 651
MlyI GAGTC 4 cut(s) 287, 332, 408, 457
MmeI TCCRAC 2 cut(s) 315, 351
MroI TCCGGA 1 cut(s) 604
MseI TTAA 1 cut(s) 105
MspA1I CMGCKG 2 cut(s) 369, 570
MspI CCGG 4 cut(s) 362, 507, 526, 605
MspR9I CCNGG 2 cut(s) 362, 645
MunI CAATTG 1 cut(s) 498
Mva1269I GAATGC 2 cut(s) 513, 640
MvaI CCWGG 1 cut(s) 645
MvnI CGCG 2 cut(s) 250, 369
MwoI GCNNNNNNNGC 2 cut(s) 247, 442
NciI CCSGG 1 cut(s) 362
NcoI CCATGG 1 cut(s) 174
NdeII GATC 1 cut(s) 379
NlaIII CATG 2 cut(s) 178, 493
NlaIV GGNNCC 1 cut(s) 505
NmeAIII GCCGAG 1 cut(s) 594
NmuCI GTSAC 2 cut(s) 283, 484
NspV TTCGAA 1 cut(s) 517
PaeR7I CTCGAG 1 cut(s) 389
PaqCI CACCTGC 2 cut(s) 427, 472
PciSI GCTCTTC 1 cut(s) 464
PcsI WCGNNNNNNNCGW 2 cut(s) 288, 402
PctI GAATGC 2 cut(s) 513, 640
PflMI CCANNNNNTGG 1 cut(s) 704
PkrI GCNGC 3 cut(s) 149, 387, 512
PleI GAGTC 4 cut(s) 287, 331, 407, 456
PpsI GAGTC 4 cut(s) 287, 331, 407, 456
PsiI TTATAA 1 cut(s) 417
Psp124BI GAGCTC 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 643
PspEI GGTNACC 1 cut(s) 484
PspFI CCCAGC 1 cut(s) 230
PspGI CCWGG 1 cut(s) 643
PspN4I GGNNCC 1 cut(s) 505
PspPI GGNCC 5 cut(s) 36, 316, 364, 504, 531
Rsr2I CGGWCCG 1 cut(s) 364
RsrII CGGWCCG 1 cut(s) 364
SacI GAGCTC 1 cut(s) 138
SacII CCGCGG 1 cut(s) 370
SalI GTCGAC 1 cut(s) 401
SapI GCTCTTC 1 cut(s) 464
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 3 cut(s) 148, 386, 511
Sau3AI GATC 1 cut(s) 379
Sau96I GGNCC 5 cut(s) 36, 316, 364, 504, 531
SchI GAGTC 4 cut(s) 287, 332, 408, 457
ScrFI CCNGG 2 cut(s) 362, 645
SduI GDGCHC 5 cut(s) 54, 138, 243, 306, 804
SfaNI GCATC 1 cut(s) 769
Sfr274I CTCGAG 1 cut(s) 389
Sfr303I CCGCGG 1 cut(s) 370
SfuI TTCGAA 1 cut(s) 517
SgrBI CCGCGG 1 cut(s) 370
SinI GGWCC 3 cut(s) 316, 364, 504
SlaI CTCGAG 1 cut(s) 389
SmlI CTYRAG 2 cut(s) 187, 389
SmoI CTYRAG 2 cut(s) 187, 389
Sse9I AATT 3 cut(s) 62, 498, 651
SsiI CCGC 8 cut(s) 351, 367, 369, 386, 474, 510, 568, 626
SspMI CTAG 2 cut(s) 131, 320
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 2 cut(s) 360, 643
StyI CCWWGG 2 cut(s) 97, 174
TaaI ACNGT 2 cut(s) 238, 793
TaiI ACGT 2 cut(s) 285, 408
TaqI TCGA 4 cut(s) 390, 402, 517, 715
TaqII GACCGA 1 cut(s) 557
TasI AATT 3 cut(s) 62, 498, 651
TauI GCSGC 2 cut(s) 388, 513
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TscAI CASTG 2 cut(s) 587, 697
TseFI GTSAC 2 cut(s) 283, 484
TseI GCWGC 1 cut(s) 147
Tsp45I GTSAC 2 cut(s) 283, 484
TspGWI ACGGA 3 cut(s) 411, 441, 569
TspRI CASTG 2 cut(s) 587, 697
Van91I CCANNNNNTGG 1 cut(s) 704
VpaK11BI GGWCC 3 cut(s) 316, 364, 504
XapI RAATTY 1 cut(s) 62
XcmI CCANNNNNNNNNTGG 1 cut(s) 571
XhoI CTCGAG 1 cut(s) 389
XmiI GTMKAC 1 cut(s) 402
XspI CTAG 2 cut(s) 131, 320
ZraI GACGTC 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.