MD06G1209800.v1.1

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014454
Physical Location & Seq
Reverse (-)
34294602 .. 34296855
2254 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1209800.v1.1.491

Sequence Viewer

Length: 684 bp
ATGGCTTCCTCCTCCATGGCCACGCTCTCGAGGACGCTTTCTCGCTCTCTGCAAGCAAAGTCCCGGACTTTACTCTCCATGCGCTTCTACACCACCGGCGCCTCCTCCTCCAGCAGCGACAGCTCCGACTCGGACGAGTCCAACCCCCTCAACTCGGAGGCAGAGTCAGCCCCGGGCGACGCCCCCCCACAGCCGCCTCCGTCCGAGGAACCCAGTCGGAGAAACTTCATGTATGATAACCCGCTCGAGAACGGCCTCGACGCCGGCATTTATAAGGCGATTCTGGTCGGGCAGGTGGGGCAGAGTCCGTTGCAGAAGAAGCTGAGGAGCGGGACCACCGTCACCATGTTTTCGGTTGGAACCGGCGGGATTCGAAACAACCGGCGGCCGTTGGATAATGAGGACCCGGTGGAGTATGCGAACCGCTGCAACGTCCAGTGGCACCGGGTTTGCGTTTATCCGGACCGGCTTAGCATGATTGCCATGAAGAATGTTGTGCCTGGTTCGATTCTATATTTGGAAGGTAATCTGGAGACCAAAATCTTCACTGATCCGGTTAGTGGTCTTGTTCGACGAGTTAGAGAGATTGCTGTTCGCAGAAACGGTCGGCTTGTGTTTTTGGGTAAAGGTGATGGTGCCGAGCAATCACCAAAATGGGGGCTCAAACGTGTTGGCTACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03430 ko03440 map03030 map03430 map03440
Pfam Domains
Protein Families
SSB

Protein Analysis

228

Amino Acids

24.92

Weight (kDa)

9.41

Isoelectric Point (pI)

66.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 92 - 199 1.2e-13 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014454)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18580
fragaria_vesca FvH4_5g12820 FvH4_5g12820
malus_domestica MD06G1209800.v1.1 MD14G1220600.v1.1
prunus_persica Prupe.5G215900_v2.0.a1
pyrus_communis pycom06g18740
rosa_chinensis RchiOBHm_Chr7g0184411
rosa_laevigata RLG00000004977
rosa_multiflora Rmu_co8454851.1_g000001 Rmu_sc0001557.1_g000018
rosa_roxburghii Rroxscaffold_3G00269650
rosa_samantha Rh7AG080200 Rh7BG067000 Rh7CG067300 Rh7DG067000
rosa_wichuraiana Rw7G005510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 273
AarI CACCTGC 1 cut(s) 283
Acc36I ACCTGC 1 cut(s) 283
AccB1I GGYRCC 3 cut(s) 98, 441, 635
AccBSI CCGCTC 2 cut(s) 244, 330
AccIII TCCGGA 1 cut(s) 460
AciI CCGC 6 cut(s) 194, 242, 330, 366, 385, 424
AclWI GGATC 1 cut(s) 545
AcoI YGGCCR 2 cut(s) 18, 386
AcyI GRCGYC 3 cut(s) 99, 180, 261
AfiI CCNNNNNNNGG 3 cut(s) 154, 560, 656
AflIII ACRYGT 1 cut(s) 667
AjnI CCWGG 1 cut(s) 499
AluBI AGCT 2 cut(s) 123, 322
AluI AGCT 2 cut(s) 123, 322
Alw26I GTCTC 1 cut(s) 527
AlwI GGATC 1 cut(s) 545
Ama87I CYCGRG 3 cut(s) 28, 172, 245
Aor13HI TCCGGA 1 cut(s) 460
AoxI GGCC 3 cut(s) 18, 253, 386
ApeKI GCWGC 2 cut(s) 114, 426
AspLEI GCGC 2 cut(s) 84, 101
AspS9I GGNCC 3 cut(s) 333, 403, 463
AsuC2I CCSGG 5 cut(s) 64, 173, 174, 407, 446
AsuHPI GGTGA 3 cut(s) 334, 639, 641
AsuII TTCGAA 1 cut(s) 373
AvaI CYCGRG 3 cut(s) 28, 172, 245
AvaII GGWCC 3 cut(s) 333, 403, 463
BalI TGGCCA 1 cut(s) 20
BanI GGYRCC 3 cut(s) 98, 441, 635
BanII GRGCYC 1 cut(s) 663
BbvCI CCTCAGC 1 cut(s) 323
BbvI GCAGC 2 cut(s) 126, 413
BccI CCATC 1 cut(s) 626
BceAI ACGGC 2 cut(s) 268, 373
BciT130I CCWGG 1 cut(s) 501
BcnI CCSGG 5 cut(s) 64, 173, 174, 407, 446
BcoDI GTCTC 1 cut(s) 527
BfoI RGCGCY 1 cut(s) 102
BfuAI ACCTGC 1 cut(s) 283
BisI GCNGC 4 cut(s) 115, 194, 386, 427
BlpI GCTNAGC 1 cut(s) 470
BlsI GCNGC 4 cut(s) 116, 195, 387, 428
Bme1390I CCNGG 6 cut(s) 64, 173, 174, 407, 446, 501
Bme18I GGWCC 3 cut(s) 333, 403, 463
BmeT110I CYCGRG 3 cut(s) 28, 172, 245
BmgT120I GGNCC 3 cut(s) 333, 403, 463
BmiI GGNNCC 7 cut(s) 100, 210, 334, 361, 405, 443, 637
BmrFI CCNGG 6 cut(s) 64, 173, 174, 407, 446, 501
BmrI ACTGGG 1 cut(s) 207
BmuI ACTGGG 1 cut(s) 207
BoxI GACNNNNGTC 1 cut(s) 338
BpmI CTGGAG 2 cut(s) 94, 551
Bpu10I CCTNAGC 1 cut(s) 323
Bpu1102I GCTNAGC 1 cut(s) 470
Bpu14I TTCGAA 1 cut(s) 373
BpuMI CCSGG 5 cut(s) 64, 173, 174, 407, 446
BsaHI GRCGYC 3 cut(s) 99, 180, 261
BsaI GGTCTC 1 cut(s) 527
BsaJI CCNNGG 4 cut(s) 15, 171, 172, 204
BsaWI WCCGGW 2 cut(s) 460, 553
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 3 cut(s) 154, 560, 656
Bse118I RCCGGY 5 cut(s) 95, 263, 362, 381, 465
Bse1I ACTGG 2 cut(s) 213, 436
BseAI TCCGGA 1 cut(s) 460
BseBI CCWGG 1 cut(s) 501
BseDI CCNNGG 4 cut(s) 15, 171, 172, 204
BseLI CCNNNNNNNGG 3 cut(s) 154, 560, 656
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 2 cut(s) 213, 436
BseRI GAGGAG 3 cut(s) 94, 97, 340
BseX3I CGGCCG 1 cut(s) 386
BseXI GCAGC 2 cut(s) 126, 413
Bsh1285I CGRYCG 2 cut(s) 389, 607
BshFI GGCC 3 cut(s) 20, 255, 388
BshNI GGYRCC 3 cut(s) 98, 441, 635
BsiEI CGRYCG 2 cut(s) 389, 607
BsiHKCI CYCGRG 3 cut(s) 28, 172, 245
BslFI GGGAC 2 cut(s) 46, 346
BslI CCNNNNNNNGG 3 cut(s) 154, 560, 656
BsmAI GTCTC 1 cut(s) 527
BsmFI GGGAC 2 cut(s) 46, 346
BsnI GGCC 3 cut(s) 20, 255, 388
Bso31I GGTCTC 1 cut(s) 527
BsoBI CYCGRG 3 cut(s) 28, 172, 245
Bsp119I TTCGAA 1 cut(s) 373
Bsp1286I GDGCHC 1 cut(s) 663
Bsp13I TCCGGA 1 cut(s) 460
Bsp143I GATC 1 cut(s) 550
Bsp1720I GCTNAGC 1 cut(s) 470
Bsp19I CCATGG 1 cut(s) 15
BspACI CCGC 6 cut(s) 194, 242, 330, 366, 385, 424
BspANI GGCC 3 cut(s) 20, 255, 388
BspCNI CTCAG 1 cut(s) 315
BspEI TCCGGA 1 cut(s) 460
BspLI GGNNCC 7 cut(s) 100, 210, 334, 361, 405, 443, 637
BspMI ACCTGC 1 cut(s) 283
BspPI GGATC 1 cut(s) 545
BspT104I TTCGAA 1 cut(s) 373
BspT107I GGYRCC 3 cut(s) 98, 441, 635
BspTNI GGTCTC 1 cut(s) 527
BsrBI CCGCTC 2 cut(s) 244, 330
BsrFI RCCGGY 5 cut(s) 95, 263, 362, 381, 465
BsrI ACTGG 2 cut(s) 213, 436
BssAI RCCGGY 5 cut(s) 95, 263, 362, 381, 465
BssECI CCNNGG 4 cut(s) 15, 171, 172, 204
BssMI GATC 1 cut(s) 550
BssNI GRCGYC 3 cut(s) 99, 180, 261
BssT1I CCWWGG 1 cut(s) 15
Bst2UI CCWGG 1 cut(s) 501
Bst4CI ACNGT 2 cut(s) 340, 605
BstACI GRCGYC 3 cut(s) 99, 180, 261
BstBI TTCGAA 1 cut(s) 373
BstC8I GCNNGC 2 cut(s) 54, 265
BstDEI CTNAG 2 cut(s) 323, 470
BstDSI CCRYGG 1 cut(s) 15
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 2 cut(s) 84, 101
BstKTI GATC 1 cut(s) 553
BstMAI GTCTC 1 cut(s) 527
BstMBI GATC 1 cut(s) 550
BstMCI CGRYCG 2 cut(s) 389, 607
BstMWI GCNNNNNNNGC 4 cut(s) 120, 167, 298, 319
BstNI CCWGG 1 cut(s) 501
BstPAI GACNNNNGTC 1 cut(s) 338
BstSCI CCNGG 6 cut(s) 62, 171, 172, 405, 444, 499
BstV1I GCAGC 2 cut(s) 126, 413
BstZI CGGCCG 1 cut(s) 386
BsuRI GGCC 3 cut(s) 20, 255, 388
BtgI CCRYGG 1 cut(s) 15
BtsIMutI CAGTG 2 cut(s) 443, 546
BveI ACCTGC 1 cut(s) 283
Cac8I GCNNGC 2 cut(s) 54, 265
CfoI GCGC 2 cut(s) 84, 101
Cfr10I RCCGGY 5 cut(s) 95, 263, 362, 381, 465
Cfr13I GGNCC 3 cut(s) 333, 403, 463
Cfr9I CCCGGG 1 cut(s) 172
CpoI CGGWCCG 1 cut(s) 463
CseI GACGC 3 cut(s) 43, 188, 269
CspI CGGWCCG 1 cut(s) 463
CviAII CATG 6 cut(s) 16, 79, 229, 346, 475, 484
DdeI CTNAG 2 cut(s) 323, 470
DinI GGCGCC 1 cut(s) 100
DpnI GATC 1 cut(s) 552
DpnII GATC 1 cut(s) 550
EaeI YGGCCR 2 cut(s) 18, 386
EagI CGGCCG 1 cut(s) 386
EclXI CGGCCG 1 cut(s) 386
Eco130I CCWWGG 1 cut(s) 15
Eco24I GRGCYC 1 cut(s) 663
Eco31I GGTCTC 1 cut(s) 527
Eco47I GGWCC 3 cut(s) 333, 403, 463
Eco52I CGGCCG 1 cut(s) 386
Eco88I CYCGRG 3 cut(s) 28, 172, 245
EcoO109I RGGNCCY 1 cut(s) 403
EcoRII CCWGG 1 cut(s) 499
EcoT14I CCWWGG 1 cut(s) 15
EcoT38I GRGCYC 1 cut(s) 663
EgeI GGCGCC 1 cut(s) 100
EheI GGCGCC 1 cut(s) 100
ErhI CCWWGG 1 cut(s) 15
FaeI CATG 6 cut(s) 19, 82, 232, 349, 478, 487
FaqI GGGAC 2 cut(s) 46, 346
FatI CATG 6 cut(s) 15, 78, 228, 345, 474, 483
FauI CCCGC 3 cut(s) 249, 323, 359
Fnu4HI GCNGC 4 cut(s) 115, 194, 386, 427
FriOI GRGCYC 1 cut(s) 663
Fsp4HI GCNGC 4 cut(s) 115, 194, 386, 427
GlaI GCGC 2 cut(s) 83, 100
GluI GCNGC 4 cut(s) 115, 194, 386, 427
GsuI CTGGAG 2 cut(s) 94, 551
HaeII RGCGCY 1 cut(s) 102
HaeIII GGCC 3 cut(s) 20, 255, 388
HgaI GACGC 3 cut(s) 43, 188, 269
HhaI GCGC 2 cut(s) 84, 101
Hin1I GRCGYC 3 cut(s) 99, 180, 261
Hin1II CATG 6 cut(s) 19, 82, 232, 349, 478, 487
Hin6I GCGC 2 cut(s) 82, 99
HinP1I GCGC 2 cut(s) 82, 99
HinfI GANTC 7 cut(s) 128, 137, 164, 280, 304, 370, 508
HphI GGTGA 3 cut(s) 334, 639, 641
Hpy188I TCNGA 5 cut(s) 127, 133, 157, 205, 219
Hpy188III TCNNGA 4 cut(s) 28, 247, 461, 530
Hpy99I CGWCG 3 cut(s) 182, 263, 576
HpyAV CCTTC 1 cut(s) 515
HpyCH4III ACNGT 2 cut(s) 340, 605
HpyCH4IV ACGT 2 cut(s) 432, 667
HpyCH4V TGCA 3 cut(s) 52, 313, 429
HpyF10VI GCNNNNNNNGC 4 cut(s) 120, 167, 298, 319
HpyF3I CTNAG 2 cut(s) 323, 470
HpySE526I ACGT 2 cut(s) 432, 667
Hsp92I GRCGYC 3 cut(s) 99, 180, 261
Hsp92II CATG 6 cut(s) 19, 82, 232, 349, 478, 487
HspAI GCGC 2 cut(s) 82, 99
KasI GGCGCC 1 cut(s) 98
Kpn2I TCCGGA 1 cut(s) 460
KroI GCCGGC 1 cut(s) 263
KroNI GCCGGC 1 cut(s) 265
Kzo9I GATC 1 cut(s) 550
LmnI GCTCC 2 cut(s) 128, 327
Lsp1109I GCAGC 2 cut(s) 126, 413
MaeII ACGT 2 cut(s) 432, 667
MaeIII GTNAC 1 cut(s) 340
MalI GATC 1 cut(s) 552
MbiI CCGCTC 2 cut(s) 244, 330
MboI GATC 1 cut(s) 550
MboII GAAGA 3 cut(s) 328, 499, 535
MhlI GDGCHC 1 cut(s) 663
MlsI TGGCCA 1 cut(s) 20
MluNI TGGCCA 1 cut(s) 20
Mly113I GGCGCC 1 cut(s) 99
MlyI GAGTC 4 cut(s) 122, 146, 173, 313
MmeI TCCRAC 5 cut(s) 150, 165, 197, 337, 372
Mox20I TGGCCA 1 cut(s) 20
MroI TCCGGA 1 cut(s) 460
MroNI GCCGGC 1 cut(s) 263
MscI TGGCCA 1 cut(s) 20
MslI CAYNNNNRTG 1 cut(s) 652
Msp20I TGGCCA 1 cut(s) 20
MspA1I CMGCKG 1 cut(s) 426
MspR9I CCNGG 6 cut(s) 64, 173, 174, 407, 446, 501
MvaI CCWGG 1 cut(s) 501
MwoI GCNNNNNNNGC 4 cut(s) 120, 167, 298, 319
NaeI GCCGGC 1 cut(s) 265
NarI GGCGCC 1 cut(s) 99
NciI CCSGG 5 cut(s) 64, 173, 174, 407, 446
NcoI CCATGG 1 cut(s) 15
NdeII GATC 1 cut(s) 550
NgoMIV GCCGGC 1 cut(s) 263
NlaIII CATG 6 cut(s) 19, 82, 232, 349, 478, 487
NlaIV GGNNCC 7 cut(s) 100, 210, 334, 361, 405, 443, 637
NmeAIII GCCGAG 1 cut(s) 664
NmuCI GTSAC 1 cut(s) 340
NspV TTCGAA 1 cut(s) 373
PaeR7I CTCGAG 2 cut(s) 28, 245
PaqCI CACCTGC 1 cut(s) 283
PdiI GCCGGC 1 cut(s) 265
PfeI GAWTC 3 cut(s) 280, 370, 508
PfoI TCCNGGA 1 cut(s) 62
PkrI GCNGC 4 cut(s) 116, 195, 387, 428
PleI GAGTC 4 cut(s) 122, 145, 172, 312
PluTI GGCGCC 1 cut(s) 102
PpsI GAGTC 4 cut(s) 122, 145, 172, 312
PpuMI RGGWCCY 1 cut(s) 403
PshAI GACNNNNGTC 1 cut(s) 338
PsiI TTATAA 1 cut(s) 273
Psp5II RGGWCCY 1 cut(s) 403
Psp6I CCWGG 1 cut(s) 499
PspGI CCWGG 1 cut(s) 499
PspN4I GGNNCC 7 cut(s) 100, 210, 334, 361, 405, 443, 637
PspPI GGNCC 3 cut(s) 333, 403, 463
PspPPI RGGWCCY 1 cut(s) 403
RseI CAYNNNNRTG 1 cut(s) 652
Rsr2I CGGWCCG 1 cut(s) 463
RsrII CGGWCCG 1 cut(s) 463
SatI GCNGC 4 cut(s) 115, 194, 386, 427
Sau3AI GATC 1 cut(s) 550
Sau96I GGNCC 3 cut(s) 333, 403, 463
SchI GAGTC 4 cut(s) 122, 146, 173, 313
ScrFI CCNGG 6 cut(s) 64, 173, 174, 407, 446, 501
SduI GDGCHC 1 cut(s) 663
SetI ASST 7 cut(s) 125, 297, 324, 435, 526, 631, 670
SfoI GGCGCC 1 cut(s) 100
Sfr274I CTCGAG 2 cut(s) 28, 245
SfuI TTCGAA 1 cut(s) 373
SgrAI CRCCGGYG 1 cut(s) 95
SinI GGWCC 3 cut(s) 333, 403, 463
SlaI CTCGAG 2 cut(s) 28, 245
SmaI CCCGGG 1 cut(s) 174
SmiMI CAYNNNNRTG 1 cut(s) 652
SmlI CTYRAG 2 cut(s) 28, 245
SmoI CTYRAG 2 cut(s) 28, 245
SsiI CCGC 6 cut(s) 194, 242, 330, 366, 385, 424
SspDI GGCGCC 1 cut(s) 98
StyD4I CCNGG 6 cut(s) 62, 171, 172, 405, 444, 499
StyI CCWWGG 1 cut(s) 15
TaaI ACNGT 2 cut(s) 340, 605
TaiI ACGT 2 cut(s) 435, 670
TaqI TCGA 6 cut(s) 29, 246, 258, 373, 506, 571
TauI GCSGC 2 cut(s) 196, 388
TfiI GAWTC 3 cut(s) 280, 370, 508
TscAI CASTG 2 cut(s) 443, 553
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 2 cut(s) 114, 426
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 2 cut(s) 217, 500
TspGWI ACGGA 2 cut(s) 189, 297
TspMI CCCGGG 1 cut(s) 172
TspRI CASTG 2 cut(s) 443, 553
VpaK11BI GGWCC 3 cut(s) 333, 403, 463
XhoI CTCGAG 2 cut(s) 28, 245
XmaI CCCGGG 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.