RLG00000004977

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0014454
Physical Location & Seq
Forward (+)
64138483 .. 64140085
1603 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004977

Sequence Viewer

Length: 663 bp
ATGCCAGCCTCCTCCTCCATGGCTTCTCTCTCAAGGAGACTACTCCTCTCTCGCTCTCTCTACCACTCAAACCCCAGCACCGTGCTCTACACGCGTTTCTGCACCACCACCACCACCAACGTCACCTCCGACTCAGACGAGCCCATCACCTCGGACCTAGAGTCCGACCCGACCCCATCACAGCCTTCCGCCCCCAACCCGGTCCGCGGTATCTACGATCAGCCGCTCGAGAACGGAGTCGACGTCGGCATTTATAAGGCGATACTGGTGGGGCAGGTGGGGCAGAGTCCGTTGCAGAAGAGACTGAAGAGTGGGGCGCAGGTGACCATGTTCTCAATTGGAACCGGCGGCATTCGAAACAACCGGAGGCCCTTTGAGACCGAGAACCCGACGGAGTATGCCAACCGCTGTGTGGTTCAGTGGCACAGGGTTTGTGTTTATCCGGAGAGGCTCGGCAAGATTGCGGTGCAGAATGCTTTGCCTGGTTCAATTTTATACTTGGAAGGTAATCTGGAGACCAAAATCTTCACTGACCCAACAACTGGGCTTGTTCGACGAGTTAGAGAGATTGCTATTCGCAGAAATGGTCGGCTTGTGTTTTTGGGTAAAGGAAATGATGCTGAGCCAACAGTAACGGGGCTCAGAGGTGTTGGCTACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03430 ko03440 map03030 map03430 map03440
Pfam Domains
Protein Families
SSB

Protein Analysis

221

Amino Acids

24.16

Weight (kDa)

9.4

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 86 - 193 5.4e-14 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014454)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18580
fragaria_vesca FvH4_5g12820 FvH4_5g12820
malus_domestica MD06G1209800.v1.1 MD14G1220600.v1.1
prunus_persica Prupe.5G215900_v2.0.a1
pyrus_communis pycom06g18740
rosa_chinensis RchiOBHm_Chr7g0184411
rosa_laevigata RLG00000004977
rosa_multiflora Rmu_co8454851.1_g000001 Rmu_sc0001557.1_g000018
rosa_roxburghii Rroxscaffold_3G00269650
rosa_samantha Rh7AG080200 Rh7BG067000 Rh7CG067300 Rh7DG067000
rosa_wichuraiana Rw7G005510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 255
AarI CACCTGC 2 cut(s) 265, 310
AatII GACGTC 1 cut(s) 246
Acc36I ACCTGC 2 cut(s) 265, 310
AccB7I CCANNNNNTGG 1 cut(s) 542
AccBSI CCGCTC 1 cut(s) 226
AccI GTMKAC 1 cut(s) 240
AccII CGCG 2 cut(s) 94, 207
AccIII TCCGGA 1 cut(s) 442
AciI CCGC 7 cut(s) 189, 205, 207, 224, 348, 406, 464
AcuI CTGAAG 1 cut(s) 326
AcyI GRCGYC 1 cut(s) 243
AfiI CCNNNNNNNGG 4 cut(s) 199, 206, 412, 542
AflIII ACRYGT 1 cut(s) 92
AgsI TTSAA 1 cut(s) 489
AhdI GACNNNNNGTC 1 cut(s) 160
AjnI CCWGG 1 cut(s) 481
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 4 cut(s) 31, 295, 371, 509
Ama87I CYCGRG 1 cut(s) 227
Aor13HI TCCGGA 1 cut(s) 442
AoxI GGCC 1 cut(s) 368
AspLEI GCGC 1 cut(s) 319
AspS9I GGNCC 3 cut(s) 154, 202, 369
AsuC2I CCSGG 1 cut(s) 200
AsuHPI GGTGA 3 cut(s) 115, 139, 334
AsuII TTCGAA 1 cut(s) 355
AvaI CYCGRG 1 cut(s) 227
AvaII GGWCC 2 cut(s) 154, 202
BanII GRGCYC 2 cut(s) 144, 642
Bbv12I GWGCWC 1 cut(s) 87
BccI CCATC 2 cut(s) 152, 184
BciT130I CCWGG 1 cut(s) 483
BcnI CCSGG 1 cut(s) 200
BcoDI GTCTC 4 cut(s) 31, 295, 371, 509
BfaI CTAG 1 cut(s) 158
BfuAI ACCTGC 2 cut(s) 265, 310
BisI GCNGC 2 cut(s) 224, 349
BlpI GCTNAGC 1 cut(s) 621
BlsI GCNGC 2 cut(s) 225, 350
Bme1390I CCNGG 2 cut(s) 200, 483
Bme18I GGWCC 2 cut(s) 154, 202
BmeRI GACNNNNNGTC 1 cut(s) 160
BmeT110I CYCGRG 1 cut(s) 227
BmgT120I GGNCC 3 cut(s) 154, 202, 369
BmiI GGNNCC 1 cut(s) 343
BmrFI CCNGG 2 cut(s) 200, 483
BmrI ACTGGG 1 cut(s) 552
BmsI GCATC 1 cut(s) 607
BmuI ACTGGG 1 cut(s) 552
BpmI CTGGAG 1 cut(s) 533
Bpu1102I GCTNAGC 1 cut(s) 621
Bpu14I TTCGAA 1 cut(s) 355
BpuEI CTTGAG 1 cut(s) 16
BpuMI CCSGG 1 cut(s) 200
BsaHI GRCGYC 1 cut(s) 243
BsaI GGTCTC 2 cut(s) 371, 509
BsaJI CCNNGG 3 cut(s) 18, 150, 205
BsaWI WCCGGW 2 cut(s) 363, 442
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 228, 258
Bsc4I CCNNNNNNNGG 4 cut(s) 199, 206, 412, 542
Bse118I RCCGGY 1 cut(s) 344
Bse1I ACTGG 2 cut(s) 270, 547
BseAI TCCGGA 1 cut(s) 442
BseBI CCWGG 1 cut(s) 483
BseDI CCNNGG 3 cut(s) 18, 150, 205
BseLI CCNNNNNNNGG 4 cut(s) 199, 206, 412, 542
BseMII CTCAG 3 cut(s) 147, 612, 655
BseNI ACTGG 2 cut(s) 270, 547
BseRI GAGGAG 2 cut(s) 4, 35
BseYI CCCAGC 1 cut(s) 74
BsgI GTGCAG 2 cut(s) 85, 488
Bsh1236I CGCG 2 cut(s) 94, 207
BshFI GGCC 1 cut(s) 370
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 227
BsiSI CCGG 4 cut(s) 200, 345, 364, 443
BslI CCNNNNNNNGG 4 cut(s) 199, 206, 412, 542
BsmAI GTCTC 4 cut(s) 31, 295, 371, 509
BsmI GAATGC 2 cut(s) 351, 478
BsnI GGCC 1 cut(s) 370
Bso31I GGTCTC 2 cut(s) 371, 509
BsoBI CYCGRG 1 cut(s) 227
Bsp119I TTCGAA 1 cut(s) 355
Bsp1286I GDGCHC 3 cut(s) 87, 144, 642
Bsp13I TCCGGA 1 cut(s) 442
Bsp143I GATC 1 cut(s) 217
Bsp1720I GCTNAGC 1 cut(s) 621
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 7 cut(s) 189, 205, 207, 224, 348, 406, 464
BspANI GGCC 1 cut(s) 370
BspCNI CTCAG 3 cut(s) 146, 613, 654
BspEI TCCGGA 1 cut(s) 442
BspFNI CGCG 2 cut(s) 94, 207
BspLI GGNNCC 1 cut(s) 343
BspMI ACCTGC 2 cut(s) 265, 310
BspT104I TTCGAA 1 cut(s) 355
BspTNI GGTCTC 2 cut(s) 371, 509
BsrBI CCGCTC 1 cut(s) 226
BsrFI RCCGGY 1 cut(s) 344
BsrI ACTGG 2 cut(s) 270, 547
BssAI RCCGGY 1 cut(s) 344
BssECI CCNNGG 3 cut(s) 18, 150, 205
BssMI GATC 1 cut(s) 217
BssNI GRCGYC 1 cut(s) 243
BssT1I CCWWGG 1 cut(s) 18
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 2 cut(s) 82, 631
Bst6I CTCTTC 2 cut(s) 293, 302
BstACI GRCGYC 1 cut(s) 243
BstBI TTCGAA 1 cut(s) 355
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 3 cut(s) 133, 621, 641
BstDSI CCRYGG 2 cut(s) 18, 205
BstEII GGTNACC 1 cut(s) 322
BstFNI CGCG 2 cut(s) 94, 207
BstHHI GCGC 1 cut(s) 319
BstKTI GATC 1 cut(s) 220
BstMAI GTCTC 4 cut(s) 31, 295, 371, 509
BstMBI GATC 1 cut(s) 217
BstMWI GCNNNNNNNGC 2 cut(s) 91, 280
BstNI CCWGG 1 cut(s) 483
BstPI GGTNACC 1 cut(s) 322
BstSCI CCNGG 2 cut(s) 198, 481
BstUI CGCG 2 cut(s) 94, 207
BsuRI GGCC 1 cut(s) 370
BtgI CCRYGG 2 cut(s) 18, 205
BtsIMutI CAGTG 2 cut(s) 425, 528
BveI ACCTGC 2 cut(s) 265, 310
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 1 cut(s) 319
Cfr10I RCCGGY 1 cut(s) 344
Cfr13I GGNCC 3 cut(s) 154, 202, 369
Cfr42I CCGCGG 1 cut(s) 208
CpoI CGGWCCG 1 cut(s) 202
CspI CGGWCCG 1 cut(s) 202
CviAII CATG 2 cut(s) 19, 328
DdeI CTNAG 3 cut(s) 133, 621, 641
DpnI GATC 1 cut(s) 219
DpnII GATC 1 cut(s) 217
DriI GACNNNNNGTC 1 cut(s) 160
Eam1104I CTCTTC 2 cut(s) 293, 302
Eam1105I GACNNNNNGTC 1 cut(s) 160
EarI CTCTTC 2 cut(s) 293, 302
EciI GGCGGA 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 18
Eco24I GRGCYC 2 cut(s) 144, 642
Eco31I GGTCTC 2 cut(s) 371, 509
Eco47I GGWCC 2 cut(s) 154, 202
Eco57I CTGAAG 1 cut(s) 326
Eco88I CYCGRG 1 cut(s) 227
Eco91I GGTNACC 1 cut(s) 322
EcoO109I RGGNCCY 1 cut(s) 369
EcoO65I GGTNACC 1 cut(s) 322
EcoRII CCWGG 1 cut(s) 481
EcoT14I CCWWGG 1 cut(s) 18
EcoT38I GRGCYC 2 cut(s) 144, 642
ErhI CCWWGG 1 cut(s) 18
FaeI CATG 2 cut(s) 22, 331
FaiI YATR 5 cut(s) 20, 255, 329, 399, 496
FatI CATG 2 cut(s) 18, 327
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 2 cut(s) 224, 349
FriOI GRGCYC 2 cut(s) 144, 642
Fsp4HI GCNGC 2 cut(s) 224, 349
FspBI CTAG 1 cut(s) 158
GlaI GCGC 1 cut(s) 318
GluI GCNGC 2 cut(s) 224, 349
GsaI CCCAGC 1 cut(s) 78
GsuI CTGGAG 1 cut(s) 533
HaeIII GGCC 1 cut(s) 370
HapII CCGG 4 cut(s) 200, 345, 364, 443
HhaI GCGC 1 cut(s) 319
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 2 cut(s) 22, 331
Hin6I GCGC 1 cut(s) 317
HinP1I GCGC 1 cut(s) 317
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HinfI GANTC 4 cut(s) 131, 161, 237, 286
HpaII CCGG 4 cut(s) 200, 345, 364, 443
HphI GGTGA 3 cut(s) 115, 139, 334
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 6 cut(s) 130, 136, 154, 166, 644, 662
Hpy188III TCNNGA 3 cut(s) 229, 443, 512
Hpy8I GTNNAC 1 cut(s) 241
Hpy99I CGWCG 4 cut(s) 245, 248, 394, 558
HpyAV CCTTC 2 cut(s) 195, 497
HpyCH4III ACNGT 2 cut(s) 82, 631
HpyCH4IV ACGT 2 cut(s) 120, 243
HpyCH4V TGCA 3 cut(s) 102, 295, 469
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 280
HpyF3I CTNAG 3 cut(s) 133, 621, 641
HpySE526I ACGT 2 cut(s) 120, 243
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 2 cut(s) 22, 331
HspAI GCGC 1 cut(s) 317
Kpn2I TCCGGA 1 cut(s) 442
KspI CCGCGG 1 cut(s) 208
Kzo9I GATC 1 cut(s) 217
LweI GCATC 1 cut(s) 607
MaeI CTAG 1 cut(s) 158
MaeII ACGT 2 cut(s) 120, 243
MaeIII GTNAC 3 cut(s) 121, 322, 631
MalI GATC 1 cut(s) 219
MbiI CCGCTC 1 cut(s) 226
MboI GATC 1 cut(s) 217
MboII GAAGA 3 cut(s) 310, 319, 517
MfeI CAATTG 1 cut(s) 336
MhlI GDGCHC 3 cut(s) 87, 144, 642
MluCI AATT 2 cut(s) 336, 489
MluI ACGCGT 1 cut(s) 92
MlyI GAGTC 4 cut(s) 125, 170, 246, 295
MmeI TCCRAC 2 cut(s) 153, 189
MnlI CCTC 9 cut(s) 19, 22, 25, 56, 136, 160, 360, 441, 638
MroI TCCGGA 1 cut(s) 442
MspA1I CMGCKG 2 cut(s) 207, 408
MspI CCGG 4 cut(s) 200, 345, 364, 443
MspR9I CCNGG 2 cut(s) 200, 483
MunI CAATTG 1 cut(s) 336
Mva1269I GAATGC 2 cut(s) 351, 478
MvaI CCWGG 1 cut(s) 483
MvnI CGCG 2 cut(s) 94, 207
MwoI GCNNNNNNNGC 2 cut(s) 91, 280
NciI CCSGG 1 cut(s) 200
NcoI CCATGG 1 cut(s) 18
NdeII GATC 1 cut(s) 217
NlaIII CATG 2 cut(s) 22, 331
NlaIV GGNNCC 1 cut(s) 343
NmeAIII GCCGAG 1 cut(s) 432
NmuCI GTSAC 2 cut(s) 121, 322
NspV TTCGAA 1 cut(s) 355
PaeR7I CTCGAG 1 cut(s) 227
PaqCI CACCTGC 2 cut(s) 265, 310
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 240
PctI GAATGC 2 cut(s) 351, 478
PflMI CCANNNNNTGG 1 cut(s) 542
PkrI GCNGC 2 cut(s) 225, 350
PleI GAGTC 4 cut(s) 125, 169, 245, 294
PpsI GAGTC 4 cut(s) 125, 169, 245, 294
PsiI TTATAA 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 481
PspEI GGTNACC 1 cut(s) 322
PspFI CCCAGC 1 cut(s) 74
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 1 cut(s) 343
PspPI GGNCC 3 cut(s) 154, 202, 369
Rsr2I CGGWCCG 1 cut(s) 202
RsrII CGGWCCG 1 cut(s) 202
SacII CCGCGG 1 cut(s) 208
SalI GTCGAC 1 cut(s) 239
SatI GCNGC 2 cut(s) 224, 349
Sau3AI GATC 1 cut(s) 217
Sau96I GGNCC 3 cut(s) 154, 202, 369
SchI GAGTC 4 cut(s) 125, 170, 246, 295
ScrFI CCNGG 2 cut(s) 200, 483
SduI GDGCHC 3 cut(s) 87, 144, 642
SetI ASST 9 cut(s) 123, 128, 152, 159, 246, 279, 324, 508, 649
SfaNI GCATC 1 cut(s) 607
Sfr274I CTCGAG 1 cut(s) 227
Sfr303I CCGCGG 1 cut(s) 208
SfuI TTCGAA 1 cut(s) 355
SgrBI CCGCGG 1 cut(s) 208
SinI GGWCC 2 cut(s) 154, 202
SlaI CTCGAG 1 cut(s) 227
SmlI CTYRAG 2 cut(s) 31, 227
SmoI CTYRAG 2 cut(s) 31, 227
Sse9I AATT 2 cut(s) 336, 489
SsiI CCGC 7 cut(s) 189, 205, 207, 224, 348, 406, 464
SspMI CTAG 1 cut(s) 158
StyD4I CCNGG 2 cut(s) 198, 481
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 2 cut(s) 82, 631
TaiI ACGT 2 cut(s) 123, 246
TaqI TCGA 4 cut(s) 228, 240, 355, 553
TaqII GACCGA 1 cut(s) 395
TasI AATT 2 cut(s) 336, 489
TauI GCSGC 2 cut(s) 226, 351
TscAI CASTG 2 cut(s) 425, 535
TseFI GTSAC 2 cut(s) 121, 322
Tsp45I GTSAC 2 cut(s) 121, 322
TspGWI ACGGA 3 cut(s) 249, 279, 407
TspRI CASTG 2 cut(s) 425, 535
Van91I CCANNNNNTGG 1 cut(s) 542
VpaK11BI GGWCC 2 cut(s) 154, 202
XcmI CCANNNNNNNNNTGG 1 cut(s) 409
XhoI CTCGAG 1 cut(s) 227
XmiI GTMKAC 1 cut(s) 240
XspI CTAG 1 cut(s) 158
ZraI GACGTC 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.