MD14G1220600.v1.1

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014454
Physical Location & Seq
Reverse (-)
30248939 .. 30250783
1845 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1220600.v1.1.491

Sequence Viewer

Length: 699 bp
ATGGCTTCCTCCTCCATGGCCACGCTCTCGAGGACGCTCTCTCGTTCTATGCAAGCAAACTCCAGGACTTTACTCTCCATGCGCTTCTACACCACCACTACCACCATCACTGGCACCTCCTCCTCCAGCAGTGACAGCTCCGACTCGGACGAGTCCAATCCTCTCAATTCAGAGGCAGAGTCAGCCCCGGGCGACGCCTCCCAACCGCCGCCCCCGTCCGATGAACCCGTTCGAAGAAGCTTCATCTACGATCGCCAGCTCGAGAACGGCCTCGACGCCGGCATTTATAAGGCAATTTTGGTGGGGCAGGTGGGGCAGAGTCCTTTGCAGAAGAGGCTGAAGAGCGGGGCATCGGTGACCATGTTTTCAATTGGGACCGGCGGGATTCGGAACAACCGGCGGCCGTTGGATAACGAGGACCCGGTGGAGTATGCGAACCGCTGCAACGTCCAGTGGCACCGCGTGTGCGTTTATCCGGACCGGCTTAGCATGATTGCCATGAAGAATGTTGTGCCTGGTTCGATTATATATTTGGAAGGTAATCTGGAGACTAAAATCTTTACTGATCCGGTTAGTGGGCTTGTTCGACGAGTTAGAGAGATTTCTATTCGCAGAAACGGTCGCCTTGTGTTTTTGGGCAAAGGTGATGGTGCTGAGCAATCACCAAAATGGGGGCTCAAAGGTGTTGGCTACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03430 ko03440 map03030 map03430 map03440
Pfam Domains
Protein Families
SSB

Protein Analysis

233

Amino Acids

25.39

Weight (kDa)

9.3

Isoelectric Point (pI)

57.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 97 - 204 1.7e-13 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014454)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18580
fragaria_vesca FvH4_5g12820 FvH4_5g12820
malus_domestica MD06G1209800.v1.1 MD14G1220600.v1.1
prunus_persica Prupe.5G215900_v2.0.a1
pyrus_communis pycom06g18740
rosa_chinensis RchiOBHm_Chr7g0184411
rosa_laevigata RLG00000004977
rosa_multiflora Rmu_co8454851.1_g000001 Rmu_sc0001557.1_g000018
rosa_roxburghii Rroxscaffold_3G00269650
rosa_samantha Rh7AG080200 Rh7BG067000 Rh7CG067300 Rh7DG067000
rosa_wichuraiana Rw7G005510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 288
AarI CACCTGC 1 cut(s) 298
Acc36I ACCTGC 1 cut(s) 298
AccB1I GGYRCC 2 cut(s) 113, 456
AccBSI CCGCTC 1 cut(s) 345
AccII CGCG 1 cut(s) 462
AccIII TCCGGA 1 cut(s) 475
AciI CCGC 7 cut(s) 206, 209, 345, 381, 400, 439, 460
AclWI GGATC 1 cut(s) 560
AcoI YGGCCR 2 cut(s) 18, 401
AcuI CTGAAG 1 cut(s) 359
AcyI GRCGYC 2 cut(s) 195, 276
AdeI CACNNNGTG 1 cut(s) 463
AfiI CCNNNNNNNGG 2 cut(s) 575, 671
AgsI TTSAA 1 cut(s) 369
AjnI CCWGG 2 cut(s) 62, 514
AluBI AGCT 3 cut(s) 138, 240, 259
AluI AGCT 3 cut(s) 138, 240, 259
Alw26I GTCTC 1 cut(s) 542
AlwI GGATC 1 cut(s) 560
Ama87I CYCGRG 3 cut(s) 28, 187, 260
Aor13HI TCCGGA 1 cut(s) 475
AoxI GGCC 3 cut(s) 18, 268, 401
ApeKI GCWGC 1 cut(s) 441
Asp700I GAANNNNTTC 1 cut(s) 228
AspLEI GCGC 1 cut(s) 84
AspS9I GGNCC 3 cut(s) 375, 418, 478
AsuC2I CCSGG 3 cut(s) 188, 189, 422
AsuHPI GGTGA 3 cut(s) 367, 654, 656
AsuII TTCGAA 1 cut(s) 232
AvaI CYCGRG 3 cut(s) 28, 187, 260
AvaII GGWCC 3 cut(s) 375, 418, 478
BalI TGGCCA 1 cut(s) 20
BanI GGYRCC 2 cut(s) 113, 456
BanII GRGCYC 1 cut(s) 678
BbvI GCAGC 1 cut(s) 428
BccI CCATC 2 cut(s) 113, 641
BceAI ACGGC 2 cut(s) 283, 388
BciT130I CCWGG 2 cut(s) 64, 516
BcnI CCSGG 3 cut(s) 188, 189, 422
BcoDI GTCTC 1 cut(s) 542
BfuAI ACCTGC 1 cut(s) 298
BisI GCNGC 3 cut(s) 209, 401, 442
BlpI GCTNAGC 2 cut(s) 485, 654
BlsI GCNGC 3 cut(s) 210, 402, 443
Bme1390I CCNGG 5 cut(s) 64, 188, 189, 422, 516
Bme18I GGWCC 3 cut(s) 375, 418, 478
BmeT110I CYCGRG 3 cut(s) 28, 187, 260
BmgT120I GGNCC 3 cut(s) 375, 418, 478
BmiI GGNNCC 4 cut(s) 115, 376, 420, 458
BmrFI CCNGG 5 cut(s) 64, 188, 189, 422, 516
BmsI GCATC 1 cut(s) 359
BpmI CTGGAG 3 cut(s) 46, 109, 566
Bpu1102I GCTNAGC 2 cut(s) 485, 654
Bpu14I TTCGAA 1 cut(s) 232
BpuMI CCSGG 3 cut(s) 188, 189, 422
BsaHI GRCGYC 2 cut(s) 195, 276
BsaJI CCNNGG 3 cut(s) 15, 186, 187
BsaWI WCCGGW 2 cut(s) 475, 568
Bsc4I CCNNNNNNNGG 2 cut(s) 575, 671
Bse118I RCCGGY 4 cut(s) 278, 377, 396, 480
Bse1I ACTGG 2 cut(s) 115, 451
BseAI TCCGGA 1 cut(s) 475
BseBI CCWGG 2 cut(s) 64, 516
BseDI CCNNGG 3 cut(s) 15, 186, 187
BseLI CCNNNNNNNGG 2 cut(s) 575, 671
BseMII CTCAG 1 cut(s) 645
BseNI ACTGG 2 cut(s) 115, 451
BseRI GAGGAG 2 cut(s) 109, 112
BseX3I CGGCCG 1 cut(s) 401
BseXI GCAGC 1 cut(s) 428
Bsh1236I CGCG 1 cut(s) 462
Bsh1285I CGRYCG 3 cut(s) 253, 404, 622
BshFI GGCC 3 cut(s) 20, 270, 403
BshNI GGYRCC 2 cut(s) 113, 456
BsiEI CGRYCG 3 cut(s) 253, 404, 622
BsiHKCI CYCGRG 3 cut(s) 28, 187, 260
BsiSI CCGG 8 cut(s) 188, 279, 378, 397, 422, 476, 481, 569
BslFI GGGAC 1 cut(s) 388
BslI CCNNNNNNNGG 2 cut(s) 575, 671
BsmAI GTCTC 1 cut(s) 542
BsmFI GGGAC 1 cut(s) 388
BsnI GGCC 3 cut(s) 20, 270, 403
BsoBI CYCGRG 3 cut(s) 28, 187, 260
Bsp119I TTCGAA 1 cut(s) 232
Bsp1286I GDGCHC 1 cut(s) 678
Bsp13I TCCGGA 1 cut(s) 475
Bsp143I GATC 2 cut(s) 250, 565
Bsp1720I GCTNAGC 2 cut(s) 485, 654
Bsp19I CCATGG 1 cut(s) 15
BspACI CCGC 7 cut(s) 206, 209, 345, 381, 400, 439, 460
BspANI GGCC 3 cut(s) 20, 270, 403
BspCNI CTCAG 1 cut(s) 646
BspEI TCCGGA 1 cut(s) 475
BspFNI CGCG 1 cut(s) 462
BspLI GGNNCC 4 cut(s) 115, 376, 420, 458
BspMI ACCTGC 1 cut(s) 298
BspPI GGATC 1 cut(s) 560
BspQI GCTCTTC 1 cut(s) 335
BspT104I TTCGAA 1 cut(s) 232
BspT107I GGYRCC 2 cut(s) 113, 456
BsrBI CCGCTC 1 cut(s) 345
BsrFI RCCGGY 4 cut(s) 278, 377, 396, 480
BsrI ACTGG 2 cut(s) 115, 451
BssAI RCCGGY 4 cut(s) 278, 377, 396, 480
BssECI CCNNGG 3 cut(s) 15, 186, 187
BssMI GATC 2 cut(s) 250, 565
BssNI GRCGYC 2 cut(s) 195, 276
BssT1I CCWWGG 1 cut(s) 15
Bst2UI CCWGG 2 cut(s) 64, 516
Bst4CI ACNGT 1 cut(s) 620
Bst6I CTCTTC 2 cut(s) 326, 335
BstACI GRCGYC 2 cut(s) 195, 276
BstBI TTCGAA 1 cut(s) 232
BstC8I GCNNGC 3 cut(s) 54, 257, 280
BstDEI CTNAG 2 cut(s) 485, 654
BstDSI CCRYGG 1 cut(s) 15
BstEII GGTNACC 1 cut(s) 355
BstFNI CGCG 1 cut(s) 462
BstHHI GCGC 1 cut(s) 84
BstKTI GATC 2 cut(s) 253, 568
BstMAI GTCTC 1 cut(s) 542
BstMBI GATC 2 cut(s) 250, 565
BstMCI CGRYCG 3 cut(s) 253, 404, 622
BstMWI GCNNNNNNNGC 4 cut(s) 135, 182, 313, 334
BstNI CCWGG 2 cut(s) 64, 516
BstPI GGTNACC 1 cut(s) 355
BstSCI CCNGG 5 cut(s) 62, 186, 187, 420, 514
BstUI CGCG 1 cut(s) 462
BstV1I GCAGC 1 cut(s) 428
BstZI CGGCCG 1 cut(s) 401
BsuRI GGCC 3 cut(s) 20, 270, 403
BtgI CCRYGG 1 cut(s) 15
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 3 cut(s) 108, 136, 458
BveI ACCTGC 1 cut(s) 298
Cac8I GCNNGC 3 cut(s) 54, 257, 280
CfoI GCGC 1 cut(s) 84
Cfr10I RCCGGY 4 cut(s) 278, 377, 396, 480
Cfr13I GGNCC 3 cut(s) 375, 418, 478
Cfr9I CCCGGG 1 cut(s) 187
CpoI CGGWCCG 1 cut(s) 478
CseI GACGC 3 cut(s) 43, 203, 284
CspCI CAANNNNNGTGG 2 cut(s) 282, 317
CspI CGGWCCG 1 cut(s) 478
CviAII CATG 5 cut(s) 16, 79, 361, 490, 499
DdeI CTNAG 2 cut(s) 485, 654
DpnI GATC 2 cut(s) 252, 567
DpnII GATC 2 cut(s) 250, 565
DraIII CACNNNGTG 1 cut(s) 463
EaeI YGGCCR 2 cut(s) 18, 401
EagI CGGCCG 1 cut(s) 401
Eam1104I CTCTTC 2 cut(s) 326, 335
EarI CTCTTC 2 cut(s) 326, 335
EclXI CGGCCG 1 cut(s) 401
Eco130I CCWWGG 1 cut(s) 15
Eco24I GRGCYC 1 cut(s) 678
Eco47I GGWCC 3 cut(s) 375, 418, 478
Eco52I CGGCCG 1 cut(s) 401
Eco57I CTGAAG 1 cut(s) 359
Eco88I CYCGRG 3 cut(s) 28, 187, 260
Eco91I GGTNACC 1 cut(s) 355
EcoO109I RGGNCCY 1 cut(s) 418
EcoO65I GGTNACC 1 cut(s) 355
EcoRII CCWGG 2 cut(s) 62, 514
EcoT14I CCWWGG 1 cut(s) 15
EcoT38I GRGCYC 1 cut(s) 678
ErhI CCWWGG 1 cut(s) 15
FaeI CATG 5 cut(s) 19, 82, 364, 493, 502
FaqI GGGAC 1 cut(s) 388
FatI CATG 5 cut(s) 15, 78, 360, 489, 498
FauI CCCGC 2 cut(s) 338, 374
Fnu4HI GCNGC 3 cut(s) 209, 401, 442
FriOI GRGCYC 1 cut(s) 678
Fsp4HI GCNGC 3 cut(s) 209, 401, 442
GlaI GCGC 1 cut(s) 83
GluI GCNGC 3 cut(s) 209, 401, 442
GsuI CTGGAG 3 cut(s) 46, 109, 566
HaeIII GGCC 3 cut(s) 20, 270, 403
HapII CCGG 8 cut(s) 188, 279, 378, 397, 422, 476, 481, 569
HgaI GACGC 3 cut(s) 43, 203, 284
HhaI GCGC 1 cut(s) 84
Hin1I GRCGYC 2 cut(s) 195, 276
Hin1II CATG 5 cut(s) 19, 82, 364, 493, 502
Hin6I GCGC 1 cut(s) 82
HinP1I GCGC 1 cut(s) 82
HindIII AAGCTT 1 cut(s) 238
HinfI GANTC 5 cut(s) 143, 152, 179, 319, 385
HpaII CCGG 8 cut(s) 188, 279, 378, 397, 422, 476, 481, 569
HphI GGTGA 3 cut(s) 367, 654, 656
Hpy188I TCNGA 5 cut(s) 142, 148, 172, 220, 390
Hpy188III TCNNGA 4 cut(s) 28, 262, 476, 545
Hpy99I CGWCG 3 cut(s) 197, 278, 591
HpyAV CCTTC 1 cut(s) 530
HpyCH4III ACNGT 1 cut(s) 620
HpyCH4IV ACGT 1 cut(s) 447
HpyCH4V TGCA 3 cut(s) 52, 328, 444
HpyF10VI GCNNNNNNNGC 4 cut(s) 135, 182, 313, 334
HpyF3I CTNAG 2 cut(s) 485, 654
HpySE526I ACGT 1 cut(s) 447
Hsp92I GRCGYC 2 cut(s) 195, 276
Hsp92II CATG 5 cut(s) 19, 82, 364, 493, 502
HspAI GCGC 1 cut(s) 82
Kpn2I TCCGGA 1 cut(s) 475
KroI GCCGGC 1 cut(s) 278
KroNI GCCGGC 1 cut(s) 280
Kzo9I GATC 2 cut(s) 250, 565
LguI GCTCTTC 1 cut(s) 335
LmnI GCTCC 1 cut(s) 143
Lsp1109I GCAGC 1 cut(s) 428
LweI GCATC 1 cut(s) 359
MaeII ACGT 1 cut(s) 447
MaeIII GTNAC 2 cut(s) 131, 355
MalI GATC 2 cut(s) 252, 567
MbiI CCGCTC 1 cut(s) 345
MboI GATC 2 cut(s) 250, 565
MboII GAAGA 4 cut(s) 246, 343, 352, 514
MfeI CAATTG 1 cut(s) 369
MhlI GDGCHC 1 cut(s) 678
MlsI TGGCCA 1 cut(s) 20
MluCI AATT 3 cut(s) 166, 294, 369
MluNI TGGCCA 1 cut(s) 20
MlyI GAGTC 4 cut(s) 137, 161, 188, 328
MmeI TCCRAC 2 cut(s) 165, 387
Mox20I TGGCCA 1 cut(s) 20
MroI TCCGGA 1 cut(s) 475
MroNI GCCGGC 1 cut(s) 278
MroXI GAANNNNTTC 1 cut(s) 228
MscI TGGCCA 1 cut(s) 20
MslI CAYNNNNRTG 1 cut(s) 667
Msp20I TGGCCA 1 cut(s) 20
MspA1I CMGCKG 1 cut(s) 441
MspI CCGG 8 cut(s) 188, 279, 378, 397, 422, 476, 481, 569
MspR9I CCNGG 5 cut(s) 64, 188, 189, 422, 516
MunI CAATTG 1 cut(s) 369
MvaI CCWGG 2 cut(s) 64, 516
MvnI CGCG 1 cut(s) 462
MwoI GCNNNNNNNGC 4 cut(s) 135, 182, 313, 334
NaeI GCCGGC 1 cut(s) 280
NciI CCSGG 3 cut(s) 188, 189, 422
NcoI CCATGG 1 cut(s) 15
NdeII GATC 2 cut(s) 250, 565
NgoMIV GCCGGC 1 cut(s) 278
NlaIII CATG 5 cut(s) 19, 82, 364, 493, 502
NlaIV GGNNCC 4 cut(s) 115, 376, 420, 458
NmuCI GTSAC 2 cut(s) 131, 355
NspV TTCGAA 1 cut(s) 232
PaeR7I CTCGAG 2 cut(s) 28, 260
PaqCI CACCTGC 1 cut(s) 298
PciSI GCTCTTC 1 cut(s) 335
PdiI GCCGGC 1 cut(s) 280
PdmI GAANNNNTTC 1 cut(s) 228
PfeI GAWTC 1 cut(s) 385
PfoI TCCNGGA 1 cut(s) 62
PkrI GCNGC 3 cut(s) 210, 402, 443
Ple19I CGATCG 1 cut(s) 253
PleI GAGTC 4 cut(s) 137, 160, 187, 327
PpsI GAGTC 4 cut(s) 137, 160, 187, 327
PpuMI RGGWCCY 1 cut(s) 418
PsiI TTATAA 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 418
Psp6I CCWGG 2 cut(s) 62, 514
PspEI GGTNACC 1 cut(s) 355
PspGI CCWGG 2 cut(s) 62, 514
PspN4I GGNNCC 4 cut(s) 115, 376, 420, 458
PspPI GGNCC 3 cut(s) 375, 418, 478
PspPPI RGGWCCY 1 cut(s) 418
PvuI CGATCG 1 cut(s) 253
RseI CAYNNNNRTG 1 cut(s) 667
Rsr2I CGGWCCG 1 cut(s) 478
RsrII CGGWCCG 1 cut(s) 478
SapI GCTCTTC 1 cut(s) 335
SatI GCNGC 3 cut(s) 209, 401, 442
Sau3AI GATC 2 cut(s) 250, 565
Sau96I GGNCC 3 cut(s) 375, 418, 478
SchI GAGTC 4 cut(s) 137, 161, 188, 328
ScrFI CCNGG 5 cut(s) 64, 188, 189, 422, 516
SduI GDGCHC 1 cut(s) 678
SetI ASST 9 cut(s) 119, 140, 242, 261, 312, 450, 541, 646, 685
SfaNI GCATC 1 cut(s) 359
Sfr274I CTCGAG 2 cut(s) 28, 260
SfuI TTCGAA 1 cut(s) 232
SinI GGWCC 3 cut(s) 375, 418, 478
SlaI CTCGAG 2 cut(s) 28, 260
SmaI CCCGGG 1 cut(s) 189
SmiMI CAYNNNNRTG 1 cut(s) 667
SmlI CTYRAG 2 cut(s) 28, 260
SmoI CTYRAG 2 cut(s) 28, 260
Sse9I AATT 3 cut(s) 166, 294, 369
SsiI CCGC 7 cut(s) 206, 209, 345, 381, 400, 439, 460
StyD4I CCNGG 5 cut(s) 62, 186, 187, 420, 514
StyI CCWWGG 1 cut(s) 15
TaaI ACNGT 1 cut(s) 620
TaiI ACGT 1 cut(s) 450
TaqI TCGA 6 cut(s) 29, 232, 261, 273, 521, 586
TasI AATT 3 cut(s) 166, 294, 369
TauI GCSGC 2 cut(s) 211, 403
TfiI GAWTC 1 cut(s) 385
TscAI CASTG 3 cut(s) 115, 136, 458
TseFI GTSAC 2 cut(s) 131, 355
TseI GCWGC 1 cut(s) 441
Tsp45I GTSAC 2 cut(s) 131, 355
TspDTI ATGAA 3 cut(s) 232, 237, 515
TspMI CCCGGG 1 cut(s) 187
TspRI CASTG 3 cut(s) 115, 136, 458
VpaK11BI GGWCC 3 cut(s) 375, 418, 478
XhoI CTCGAG 2 cut(s) 28, 260
XmaI CCCGGG 1 cut(s) 187
XmnI GAANNNNTTC 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.