Rmu_co8454851.1_g000001

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8454851.1
OG0014454
Physical Location & Seq
Reverse (-)
1 .. 1466
1466 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8454851.1_g000001.1.cds

Sequence Viewer

Length: 473 bp
atgtcagcctcctcctccatggcttcactctcaaggagactactcctctctcgctctctctaccactcaaaccccagcaccgtgctctccacgcgcttctgcaccaccaccaccaccaccccccacgtcacctccgactcagacgagcccatcacctcggacctagagtccgacccgaccccatcacagccctccgcccccaacccggtccgcggtatctacgatcagccgctcgagaacggagtcgacgtcggcatttataaggtggggcagagtccgttgcagaagagactgaagagtggggcgcaggtgaccatgttctcaattgggaccggcggcattcgaaacaaccggaggccctttgagaccgagaacccgacggagtatgccaaccgctgtgtggttcagtggcacagggtttgtgtttatccggagaggctcggcaagattgcggtgcagaatgctttgcctgg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03430 ko03440 map03030 map03430 map03440
Pfam Domains
Protein Families
SSB

Protein Analysis

158

Amino Acids

17.28

Weight (kDa)

8.91

Isoelectric Point (pI)

55.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014454)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18580
fragaria_vesca FvH4_5g12820 FvH4_5g12820
malus_domestica MD06G1209800.v1.1 MD14G1220600.v1.1
prunus_persica Prupe.5G215900_v2.0.a1
pyrus_communis pycom06g18740
rosa_chinensis RchiOBHm_Chr7g0184411
rosa_laevigata RLG00000004977
rosa_multiflora Rmu_co8454851.1_g000001 Rmu_sc0001557.1_g000018
rosa_roxburghii Rroxscaffold_3G00269650
rosa_samantha Rh7AG080200 Rh7BG067000 Rh7CG067300 Rh7DG067000
rosa_wichuraiana Rw7G005510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 261
AarI CACCTGC 1 cut(s) 298
AatII GACGTC 1 cut(s) 252
Acc36I ACCTGC 1 cut(s) 298
AccBSI CCGCTC 1 cut(s) 232
AccI GTMKAC 1 cut(s) 246
AccII CGCG 2 cut(s) 94, 213
AccIII TCCGGA 1 cut(s) 430
AciI CCGC 7 cut(s) 195, 211, 213, 230, 336, 394, 452
AcuI CTGAAG 1 cut(s) 314
AcyI GRCGYC 1 cut(s) 249
AfiI CCNNNNNNNGG 3 cut(s) 205, 212, 400
AhdI GACNNNNNGTC 1 cut(s) 166
AjiI CACGTC 1 cut(s) 127
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 3 cut(s) 31, 283, 359
Ama87I CYCGRG 1 cut(s) 233
Aor13HI TCCGGA 1 cut(s) 430
AoxI GGCC 1 cut(s) 356
AspLEI GCGC 2 cut(s) 96, 307
AspS9I GGNCC 4 cut(s) 160, 208, 330, 357
AsuC2I CCSGG 1 cut(s) 206
AsuHPI GGTGA 3 cut(s) 121, 145, 322
AsuII TTCGAA 1 cut(s) 343
AvaI CYCGRG 1 cut(s) 233
AvaII GGWCC 3 cut(s) 160, 208, 330
BanII GRGCYC 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 87
BccI CCATC 2 cut(s) 158, 190
BciT130I CCWGG 1 cut(s) 471
BcnI CCSGG 1 cut(s) 206
BcoDI GTCTC 3 cut(s) 31, 283, 359
BfaI CTAG 1 cut(s) 164
BfuAI ACCTGC 1 cut(s) 298
BisI GCNGC 2 cut(s) 230, 337
BlsI GCNGC 2 cut(s) 231, 338
Bme1390I CCNGG 2 cut(s) 206, 471
Bme18I GGWCC 3 cut(s) 160, 208, 330
BmeRI GACNNNNNGTC 1 cut(s) 166
BmeT110I CYCGRG 1 cut(s) 233
BmgBI CACGTC 1 cut(s) 127
BmgT120I GGNCC 4 cut(s) 160, 208, 330, 357
BmiI GGNNCC 1 cut(s) 331
BmrFI CCNGG 2 cut(s) 206, 471
Bpu14I TTCGAA 1 cut(s) 343
BpuEI CTTGAG 1 cut(s) 16
BpuMI CCSGG 1 cut(s) 206
BsaHI GRCGYC 1 cut(s) 249
BsaI GGTCTC 1 cut(s) 359
BsaJI CCNNGG 3 cut(s) 18, 156, 211
BsaWI WCCGGW 2 cut(s) 351, 430
BsaXI ACNNNNNCTCC 4 cut(s) 116, 146, 234, 264
Bsc4I CCNNNNNNNGG 3 cut(s) 205, 212, 400
Bse118I RCCGGY 1 cut(s) 332
BseAI TCCGGA 1 cut(s) 430
BseBI CCWGG 1 cut(s) 471
BseDI CCNNGG 3 cut(s) 18, 156, 211
BseLI CCNNNNNNNGG 3 cut(s) 205, 212, 400
BseMII CTCAG 1 cut(s) 153
BseRI GAGGAG 2 cut(s) 4, 35
BseYI CCCAGC 1 cut(s) 74
BsgI GTGCAG 1 cut(s) 85
Bsh1236I CGCG 2 cut(s) 94, 213
BshFI GGCC 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 4 cut(s) 206, 333, 352, 431
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 3 cut(s) 205, 212, 400
BsmAI GTCTC 3 cut(s) 31, 283, 359
BsmFI GGGAC 1 cut(s) 343
BsmI GAATGC 2 cut(s) 339, 466
BsnI GGCC 1 cut(s) 358
Bso31I GGTCTC 1 cut(s) 359
BsoBI CYCGRG 1 cut(s) 233
Bsp119I TTCGAA 1 cut(s) 343
Bsp1286I GDGCHC 2 cut(s) 87, 150
Bsp13I TCCGGA 1 cut(s) 430
Bsp143I GATC 1 cut(s) 223
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 7 cut(s) 195, 211, 213, 230, 336, 394, 452
BspANI GGCC 1 cut(s) 358
BspCNI CTCAG 1 cut(s) 152
BspEI TCCGGA 1 cut(s) 430
BspFNI CGCG 2 cut(s) 94, 213
BspLI GGNNCC 1 cut(s) 331
BspMI ACCTGC 1 cut(s) 298
BspT104I TTCGAA 1 cut(s) 343
BspTNI GGTCTC 1 cut(s) 359
BsrBI CCGCTC 1 cut(s) 232
BsrFI RCCGGY 1 cut(s) 332
BssAI RCCGGY 1 cut(s) 332
BssECI CCNNGG 3 cut(s) 18, 156, 211
BssMI GATC 1 cut(s) 223
BssNI GRCGYC 1 cut(s) 249
BssT1I CCWWGG 1 cut(s) 18
Bst2UI CCWGG 1 cut(s) 471
Bst4CI ACNGT 1 cut(s) 82
Bst6I CTCTTC 2 cut(s) 281, 290
BstACI GRCGYC 1 cut(s) 249
BstBI TTCGAA 1 cut(s) 343
BstDEI CTNAG 1 cut(s) 139
BstDSI CCRYGG 2 cut(s) 18, 211
BstEII GGTNACC 1 cut(s) 310
BstFNI CGCG 2 cut(s) 94, 213
BstHHI GCGC 2 cut(s) 96, 307
BstKTI GATC 1 cut(s) 226
BstMAI GTCTC 3 cut(s) 31, 283, 359
BstMBI GATC 1 cut(s) 223
BstMWI GCNNNNNNNGC 1 cut(s) 91
BstNI CCWGG 1 cut(s) 471
BstPI GGTNACC 1 cut(s) 310
BstSCI CCNGG 1 cut(s) 204
BstUI CGCG 2 cut(s) 94, 213
BsuRI GGCC 1 cut(s) 358
BtgI CCRYGG 2 cut(s) 18, 211
BtrI CACGTC 1 cut(s) 127
BtsIMutI CAGTG 1 cut(s) 413
BveI ACCTGC 1 cut(s) 298
CfoI GCGC 2 cut(s) 96, 307
Cfr10I RCCGGY 1 cut(s) 332
Cfr13I GGNCC 4 cut(s) 160, 208, 330, 357
Cfr42I CCGCGG 1 cut(s) 214
CpoI CGGWCCG 1 cut(s) 208
CspI CGGWCCG 1 cut(s) 208
CviAII CATG 2 cut(s) 19, 316
CviJI RGCY 7 cut(s) 8, 23, 148, 190, 229, 358, 439
CviKI_1 RGCY 7 cut(s) 8, 23, 148, 190, 229, 358, 439
DdeI CTNAG 1 cut(s) 139
DpnI GATC 1 cut(s) 225
DpnII GATC 1 cut(s) 223
DriI GACNNNNNGTC 1 cut(s) 166
Eam1104I CTCTTC 2 cut(s) 281, 290
Eam1105I GACNNNNNGTC 1 cut(s) 166
EarI CTCTTC 2 cut(s) 281, 290
EciI GGCGGA 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 18
Eco24I GRGCYC 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 359
Eco47I GGWCC 3 cut(s) 160, 208, 330
Eco57I CTGAAG 1 cut(s) 314
Eco88I CYCGRG 1 cut(s) 233
Eco91I GGTNACC 1 cut(s) 310
EcoO109I RGGNCCY 1 cut(s) 357
EcoO65I GGTNACC 1 cut(s) 310
EcoT14I CCWWGG 1 cut(s) 18
EcoT38I GRGCYC 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 18
FaeI CATG 2 cut(s) 22, 319
FaiI YATR 4 cut(s) 20, 261, 317, 387
FaqI GGGAC 1 cut(s) 343
FatI CATG 2 cut(s) 18, 315
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 2 cut(s) 230, 337
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 2 cut(s) 230, 337
FspBI CTAG 1 cut(s) 164
GlaI GCGC 2 cut(s) 95, 306
GluI GCNGC 2 cut(s) 230, 337
GsaI CCCAGC 1 cut(s) 78
HaeIII GGCC 1 cut(s) 358
HapII CCGG 4 cut(s) 206, 333, 352, 431
HhaI GCGC 2 cut(s) 96, 307
Hin1I GRCGYC 1 cut(s) 249
Hin1II CATG 2 cut(s) 22, 319
Hin6I GCGC 2 cut(s) 94, 305
HinP1I GCGC 2 cut(s) 94, 305
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 4 cut(s) 137, 167, 243, 274
HpaII CCGG 4 cut(s) 206, 333, 352, 431
HphI GGTGA 3 cut(s) 121, 145, 322
Hpy166II GTNNAC 1 cut(s) 247
Hpy188I TCNGA 4 cut(s) 136, 142, 160, 172
Hpy188III TCNNGA 2 cut(s) 235, 431
Hpy8I GTNNAC 1 cut(s) 247
Hpy99I CGWCG 3 cut(s) 251, 254, 382
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 2 cut(s) 126, 249
HpyCH4V TGCA 3 cut(s) 102, 283, 457
HpyF10VI GCNNNNNNNGC 1 cut(s) 91
HpyF3I CTNAG 1 cut(s) 139
HpySE526I ACGT 2 cut(s) 126, 249
Hsp92I GRCGYC 1 cut(s) 249
Hsp92II CATG 2 cut(s) 22, 319
HspAI GCGC 2 cut(s) 94, 305
Kpn2I TCCGGA 1 cut(s) 430
KspI CCGCGG 1 cut(s) 214
Kzo9I GATC 1 cut(s) 223
LpnPI CCDG 8 cut(s) 88, 219, 293, 346, 365, 400, 444, 456
MaeI CTAG 1 cut(s) 164
MaeII ACGT 2 cut(s) 126, 249
MaeIII GTNAC 2 cut(s) 127, 310
MalI GATC 1 cut(s) 225
MbiI CCGCTC 1 cut(s) 232
MboI GATC 1 cut(s) 223
MboII GAAGA 2 cut(s) 298, 307
MfeI CAATTG 1 cut(s) 324
MhlI GDGCHC 2 cut(s) 87, 150
MluCI AATT 1 cut(s) 324
MlyI GAGTC 4 cut(s) 131, 176, 252, 283
MmeI TCCRAC 2 cut(s) 159, 195
MnlI CCTC 9 cut(s) 19, 22, 25, 56, 142, 166, 202, 348, 429
MroI TCCGGA 1 cut(s) 430
MspA1I CMGCKG 2 cut(s) 213, 396
MspI CCGG 4 cut(s) 206, 333, 352, 431
MspR9I CCNGG 2 cut(s) 206, 471
MunI CAATTG 1 cut(s) 324
Mva1269I GAATGC 2 cut(s) 339, 466
MvaI CCWGG 1 cut(s) 471
MvnI CGCG 2 cut(s) 94, 213
MwoI GCNNNNNNNGC 1 cut(s) 91
NciI CCSGG 1 cut(s) 206
NcoI CCATGG 1 cut(s) 18
NdeII GATC 1 cut(s) 223
NlaIII CATG 2 cut(s) 22, 319
NlaIV GGNNCC 1 cut(s) 331
NmeAIII GCCGAG 1 cut(s) 420
NmuCI GTSAC 2 cut(s) 127, 310
NspV TTCGAA 1 cut(s) 343
PaeR7I CTCGAG 1 cut(s) 233
PaqCI CACCTGC 1 cut(s) 298
PcsI WCGNNNNNNNCGW 2 cut(s) 132, 246
PctI GAATGC 2 cut(s) 339, 466
PkrI GCNGC 2 cut(s) 231, 338
PleI GAGTC 4 cut(s) 131, 175, 251, 282
PpsI GAGTC 4 cut(s) 131, 175, 251, 282
PsiI TTATAA 1 cut(s) 261
PspEI GGTNACC 1 cut(s) 310
PspFI CCCAGC 1 cut(s) 74
PspN4I GGNNCC 1 cut(s) 331
PspPI GGNCC 4 cut(s) 160, 208, 330, 357
Rsr2I CGGWCCG 1 cut(s) 208
RsrII CGGWCCG 1 cut(s) 208
SacII CCGCGG 1 cut(s) 214
SalI GTCGAC 1 cut(s) 245
SatI GCNGC 2 cut(s) 230, 337
Sau3AI GATC 1 cut(s) 223
Sau96I GGNCC 4 cut(s) 160, 208, 330, 357
SchI GAGTC 4 cut(s) 131, 176, 252, 283
ScrFI CCNGG 2 cut(s) 206, 471
SduI GDGCHC 2 cut(s) 87, 150
SetI ASST 7 cut(s) 129, 134, 158, 165, 252, 267, 312
Sfr274I CTCGAG 1 cut(s) 233
Sfr303I CCGCGG 1 cut(s) 214
SfuI TTCGAA 1 cut(s) 343
SgrBI CCGCGG 1 cut(s) 214
SinI GGWCC 3 cut(s) 160, 208, 330
SlaI CTCGAG 1 cut(s) 233
SmlI CTYRAG 2 cut(s) 31, 233
SmoI CTYRAG 2 cut(s) 31, 233
Sse9I AATT 1 cut(s) 324
SsiI CCGC 7 cut(s) 195, 211, 213, 230, 336, 394, 452
SspMI CTAG 1 cut(s) 164
StyD4I CCNGG 1 cut(s) 204
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 2 cut(s) 129, 252
TaqI TCGA 3 cut(s) 234, 246, 343
TaqII GACCGA 1 cut(s) 383
TasI AATT 1 cut(s) 324
TauI GCSGC 2 cut(s) 232, 339
TscAI CASTG 1 cut(s) 413
TseFI GTSAC 2 cut(s) 127, 310
Tsp45I GTSAC 2 cut(s) 127, 310
TspGWI ACGGA 3 cut(s) 255, 267, 395
TspRI CASTG 1 cut(s) 413
VpaK11BI GGWCC 3 cut(s) 160, 208, 330
XcmI CCANNNNNNNNNTGG 1 cut(s) 397
XhoI CTCGAG 1 cut(s) 233
XmiI GTMKAC 1 cut(s) 246
XspI CTAG 1 cut(s) 164
ZraI GACGTC 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.