Rh7CG067300

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0014454
Physical Location & Seq
Reverse (-)
4815527 .. 4817176
1650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG067300.1

Sequence Viewer

Length: 681 bp
ATGTCAGCCTCCTCCTCCATGGCTTCTCTCTCCAGGAGACTACTCCTCTCTCGCTCTCTCTACCACTCAAACCCCAGCACCGTGCTCTCCACGCGCTTCTGCACCACCACCACCACCACCACCACCACCACCACCCACGTCACCTCCGACTCAGACGAGCCCATCACCTCGGACCTAGAGTCCGACCCGACCCCATCACAGCCCTCCGCCCCCAACCCGGTCCGCGGTATCTACGATCAGCCTCTCGAGAACGGAGTCGACGTCGGCATTTATAAGGCGATACTGGTGGGGCAGGTGGGGCAGAGTCCGTTGCAGAAGAGACTGAAGAGTGGGGCGCAGGTGACTATGTTCTCAATTGGGACCGGCGGCATTCGAAACAACCGGAGGCCCTTTGAGACCGAGAACCCGACGGAGTATGCCAACCGCTGTGTGGTTCAGTGGCACAGGGTTTGTGTTTATCCGGAGAGGCTCGGCAAGATTGCGGTGCAGAATGCTTTGCCTGGTTCAATTTTATACTTGGAAGGTAATCTGGAGACCAAAATCTTCACTGACCCAACAACTGGGCTTGTTCGACGAGTTAGAGAGATTGCTATTCGCAGAAATGGTCGCCTTGTGTTTTTGGGTAAAGGAAACGATGCTGAGCCAACAGTAACGGGGCTCAGAGGTGTTGGCTACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03430 ko03440 map03030 map03430 map03440
Pfam Domains
Protein Families
SSB

Protein Analysis

226

Amino Acids

24.7

Weight (kDa)

9.43

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSB PF00436 92 - 199 5.7e-14 Single-strand binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014454)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18580
fragaria_vesca FvH4_5g12820 FvH4_5g12820
malus_domestica MD06G1209800.v1.1 MD14G1220600.v1.1
prunus_persica Prupe.5G215900_v2.0.a1
pyrus_communis pycom06g18740
rosa_chinensis RchiOBHm_Chr7g0184411
rosa_laevigata RLG00000004977
rosa_multiflora Rmu_co8454851.1_g000001 Rmu_sc0001557.1_g000018
rosa_roxburghii Rroxscaffold_3G00269650
rosa_samantha Rh7AG080200 Rh7BG067000 Rh7CG067300 Rh7DG067000
rosa_wichuraiana Rw7G005510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 273
AarI CACCTGC 2 cut(s) 283, 328
AatII GACGTC 1 cut(s) 264
Acc36I ACCTGC 2 cut(s) 283, 328
AccB7I CCANNNNNTGG 1 cut(s) 560
AccI GTMKAC 1 cut(s) 258
AccII CGCG 2 cut(s) 94, 225
AccIII TCCGGA 1 cut(s) 460
AciI CCGC 6 cut(s) 207, 223, 225, 366, 424, 482
AcuI CTGAAG 1 cut(s) 344
AcyI GRCGYC 1 cut(s) 261
AfiI CCNNNNNNNGG 4 cut(s) 217, 224, 430, 560
AgsI TTSAA 1 cut(s) 507
AhdI GACNNNNNGTC 1 cut(s) 178
AjiI CACGTC 1 cut(s) 139
AjnI CCWGG 2 cut(s) 32, 499
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 4 cut(s) 31, 313, 389, 527
Ama87I CYCGRG 1 cut(s) 245
Aor13HI TCCGGA 1 cut(s) 460
AoxI GGCC 1 cut(s) 386
AspLEI GCGC 2 cut(s) 96, 337
AspS9I GGNCC 4 cut(s) 172, 220, 360, 387
AsuC2I CCSGG 1 cut(s) 218
AsuHPI GGTGA 3 cut(s) 133, 157, 352
AsuII TTCGAA 1 cut(s) 373
AvaI CYCGRG 1 cut(s) 245
AvaII GGWCC 3 cut(s) 172, 220, 360
BanII GRGCYC 2 cut(s) 162, 660
Bbv12I GWGCWC 1 cut(s) 87
BccI CCATC 2 cut(s) 170, 202
BciT130I CCWGG 2 cut(s) 34, 501
BcnI CCSGG 1 cut(s) 218
BcoDI GTCTC 4 cut(s) 31, 313, 389, 527
BfaI CTAG 1 cut(s) 176
BfuAI ACCTGC 2 cut(s) 283, 328
BisI GCNGC 1 cut(s) 367
BlpI GCTNAGC 1 cut(s) 639
BlsI GCNGC 1 cut(s) 368
Bme1390I CCNGG 3 cut(s) 34, 218, 501
Bme18I GGWCC 3 cut(s) 172, 220, 360
BmeRI GACNNNNNGTC 1 cut(s) 178
BmeT110I CYCGRG 1 cut(s) 245
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 4 cut(s) 172, 220, 360, 387
BmiI GGNNCC 1 cut(s) 361
BmrFI CCNGG 3 cut(s) 34, 218, 501
BmrI ACTGGG 1 cut(s) 570
BmsI GCATC 1 cut(s) 625
BmuI ACTGGG 1 cut(s) 570
BpmI CTGGAG 2 cut(s) 16, 551
Bpu1102I GCTNAGC 1 cut(s) 639
Bpu14I TTCGAA 1 cut(s) 373
BpuMI CCSGG 1 cut(s) 218
BsaHI GRCGYC 1 cut(s) 261
BsaI GGTCTC 2 cut(s) 389, 527
BsaJI CCNNGG 3 cut(s) 18, 168, 223
BsaWI WCCGGW 2 cut(s) 381, 460
BsaXI ACNNNNNCTCC 4 cut(s) 128, 158, 246, 276
Bsc4I CCNNNNNNNGG 4 cut(s) 217, 224, 430, 560
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 2 cut(s) 288, 565
BseAI TCCGGA 1 cut(s) 460
BseBI CCWGG 2 cut(s) 34, 501
BseDI CCNNGG 3 cut(s) 18, 168, 223
BseLI CCNNNNNNNGG 4 cut(s) 217, 224, 430, 560
BseMII CTCAG 3 cut(s) 165, 630, 673
BseNI ACTGG 2 cut(s) 288, 565
BseRI GAGGAG 2 cut(s) 4, 35
BseYI CCCAGC 1 cut(s) 74
BsgI GTGCAG 2 cut(s) 85, 506
Bsh1236I CGCG 2 cut(s) 94, 225
BshFI GGCC 1 cut(s) 388
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 4 cut(s) 218, 363, 382, 461
BslFI GGGAC 1 cut(s) 373
BslI CCNNNNNNNGG 4 cut(s) 217, 224, 430, 560
BsmAI GTCTC 4 cut(s) 31, 313, 389, 527
BsmFI GGGAC 1 cut(s) 373
BsmI GAATGC 2 cut(s) 369, 496
BsnI GGCC 1 cut(s) 388
Bso31I GGTCTC 2 cut(s) 389, 527
BsoBI CYCGRG 1 cut(s) 245
Bsp119I TTCGAA 1 cut(s) 373
Bsp1286I GDGCHC 3 cut(s) 87, 162, 660
Bsp13I TCCGGA 1 cut(s) 460
Bsp143I GATC 1 cut(s) 235
Bsp1720I GCTNAGC 1 cut(s) 639
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 6 cut(s) 207, 223, 225, 366, 424, 482
BspANI GGCC 1 cut(s) 388
BspCNI CTCAG 3 cut(s) 164, 631, 672
BspEI TCCGGA 1 cut(s) 460
BspFNI CGCG 2 cut(s) 94, 225
BspLI GGNNCC 1 cut(s) 361
BspMI ACCTGC 2 cut(s) 283, 328
BspT104I TTCGAA 1 cut(s) 373
BspTNI GGTCTC 2 cut(s) 389, 527
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 2 cut(s) 288, 565
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 3 cut(s) 18, 168, 223
BssMI GATC 1 cut(s) 235
BssNI GRCGYC 1 cut(s) 261
BssT1I CCWWGG 1 cut(s) 18
Bst2UI CCWGG 2 cut(s) 34, 501
Bst4CI ACNGT 2 cut(s) 82, 649
Bst6I CTCTTC 2 cut(s) 311, 320
BstACI GRCGYC 1 cut(s) 261
BstBI TTCGAA 1 cut(s) 373
BstDEI CTNAG 3 cut(s) 151, 639, 659
BstDSI CCRYGG 2 cut(s) 18, 223
BstFNI CGCG 2 cut(s) 94, 225
BstHHI GCGC 2 cut(s) 96, 337
BstKTI GATC 1 cut(s) 238
BstMAI GTCTC 4 cut(s) 31, 313, 389, 527
BstMBI GATC 1 cut(s) 235
BstMWI GCNNNNNNNGC 2 cut(s) 91, 298
BstNI CCWGG 2 cut(s) 34, 501
BstSCI CCNGG 3 cut(s) 32, 216, 499
BstUI CGCG 2 cut(s) 94, 225
BsuRI GGCC 1 cut(s) 388
BtgI CCRYGG 2 cut(s) 18, 223
BtrI CACGTC 1 cut(s) 139
BtsIMutI CAGTG 2 cut(s) 443, 546
BveI ACCTGC 2 cut(s) 283, 328
CfoI GCGC 2 cut(s) 96, 337
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 4 cut(s) 172, 220, 360, 387
Cfr42I CCGCGG 1 cut(s) 226
CpoI CGGWCCG 1 cut(s) 220
CspI CGGWCCG 1 cut(s) 220
CviAII CATG 1 cut(s) 19
DdeI CTNAG 3 cut(s) 151, 639, 659
DpnI GATC 1 cut(s) 237
DpnII GATC 1 cut(s) 235
DriI GACNNNNNGTC 1 cut(s) 178
Eam1104I CTCTTC 2 cut(s) 311, 320
Eam1105I GACNNNNNGTC 1 cut(s) 178
EarI CTCTTC 2 cut(s) 311, 320
EciI GGCGGA 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 18
Eco24I GRGCYC 2 cut(s) 162, 660
Eco31I GGTCTC 2 cut(s) 389, 527
Eco47I GGWCC 3 cut(s) 172, 220, 360
Eco57I CTGAAG 1 cut(s) 344
Eco88I CYCGRG 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 387
EcoRII CCWGG 2 cut(s) 32, 499
EcoT14I CCWWGG 1 cut(s) 18
EcoT38I GRGCYC 2 cut(s) 162, 660
ErhI CCWWGG 1 cut(s) 18
FaeI CATG 1 cut(s) 22
FaiI YATR 5 cut(s) 20, 273, 347, 417, 514
FaqI GGGAC 1 cut(s) 373
FatI CATG 1 cut(s) 18
FblI GTMKAC 1 cut(s) 258
Fnu4HI GCNGC 1 cut(s) 367
FriOI GRGCYC 2 cut(s) 162, 660
Fsp4HI GCNGC 1 cut(s) 367
FspBI CTAG 1 cut(s) 176
GlaI GCGC 2 cut(s) 95, 336
GluI GCNGC 1 cut(s) 367
GsaI CCCAGC 1 cut(s) 78
GsuI CTGGAG 2 cut(s) 16, 551
HaeIII GGCC 1 cut(s) 388
HapII CCGG 4 cut(s) 218, 363, 382, 461
HhaI GCGC 2 cut(s) 96, 337
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 1 cut(s) 22
Hin6I GCGC 2 cut(s) 94, 335
HinP1I GCGC 2 cut(s) 94, 335
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HinfI GANTC 4 cut(s) 149, 179, 255, 304
HpaII CCGG 4 cut(s) 218, 363, 382, 461
HphI GGTGA 3 cut(s) 133, 157, 352
Hpy166II GTNNAC 1 cut(s) 259
Hpy188I TCNGA 6 cut(s) 148, 154, 172, 184, 662, 680
Hpy188III TCNNGA 4 cut(s) 245, 247, 461, 530
Hpy8I GTNNAC 1 cut(s) 259
Hpy99I CGWCG 4 cut(s) 263, 266, 412, 576
HpyAV CCTTC 1 cut(s) 515
HpyCH4III ACNGT 2 cut(s) 82, 649
HpyCH4IV ACGT 2 cut(s) 138, 261
HpyCH4V TGCA 3 cut(s) 102, 313, 487
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 298
HpyF3I CTNAG 3 cut(s) 151, 639, 659
HpySE526I ACGT 2 cut(s) 138, 261
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 1 cut(s) 22
HspAI GCGC 2 cut(s) 94, 335
Kpn2I TCCGGA 1 cut(s) 460
KspI CCGCGG 1 cut(s) 226
Kzo9I GATC 1 cut(s) 235
LweI GCATC 1 cut(s) 625
MaeI CTAG 1 cut(s) 176
MaeII ACGT 2 cut(s) 138, 261
MaeIII GTNAC 3 cut(s) 139, 340, 649
MalI GATC 1 cut(s) 237
MboI GATC 1 cut(s) 235
MboII GAAGA 3 cut(s) 328, 337, 535
MfeI CAATTG 1 cut(s) 354
MhlI GDGCHC 3 cut(s) 87, 162, 660
MluCI AATT 2 cut(s) 354, 507
MlyI GAGTC 4 cut(s) 143, 188, 264, 313
MmeI TCCRAC 2 cut(s) 171, 207
MroI TCCGGA 1 cut(s) 460
MspA1I CMGCKG 2 cut(s) 225, 426
MspI CCGG 4 cut(s) 218, 363, 382, 461
MspR9I CCNGG 3 cut(s) 34, 218, 501
MunI CAATTG 1 cut(s) 354
Mva1269I GAATGC 2 cut(s) 369, 496
MvaI CCWGG 2 cut(s) 34, 501
MvnI CGCG 2 cut(s) 94, 225
MwoI GCNNNNNNNGC 2 cut(s) 91, 298
NciI CCSGG 1 cut(s) 218
NcoI CCATGG 1 cut(s) 18
NdeII GATC 1 cut(s) 235
NlaIII CATG 1 cut(s) 22
NlaIV GGNNCC 1 cut(s) 361
NmeAIII GCCGAG 1 cut(s) 450
NmuCI GTSAC 2 cut(s) 139, 340
NspV TTCGAA 1 cut(s) 373
PaeR7I CTCGAG 1 cut(s) 245
PaqCI CACCTGC 2 cut(s) 283, 328
PcsI WCGNNNNNNNCGW 2 cut(s) 144, 258
PctI GAATGC 2 cut(s) 369, 496
PflMI CCANNNNNTGG 1 cut(s) 560
PfoI TCCNGGA 1 cut(s) 32
PkrI GCNGC 1 cut(s) 368
PleI GAGTC 4 cut(s) 143, 187, 263, 312
PpsI GAGTC 4 cut(s) 143, 187, 263, 312
PsiI TTATAA 1 cut(s) 273
Psp6I CCWGG 2 cut(s) 32, 499
PspFI CCCAGC 1 cut(s) 74
PspGI CCWGG 2 cut(s) 32, 499
PspN4I GGNNCC 1 cut(s) 361
PspPI GGNCC 4 cut(s) 172, 220, 360, 387
Rsr2I CGGWCCG 1 cut(s) 220
RsrII CGGWCCG 1 cut(s) 220
SacII CCGCGG 1 cut(s) 226
SalI GTCGAC 1 cut(s) 257
SatI GCNGC 1 cut(s) 367
Sau3AI GATC 1 cut(s) 235
Sau96I GGNCC 4 cut(s) 172, 220, 360, 387
SchI GAGTC 4 cut(s) 143, 188, 264, 313
ScrFI CCNGG 3 cut(s) 34, 218, 501
SduI GDGCHC 3 cut(s) 87, 162, 660
SetI ASST 9 cut(s) 141, 146, 170, 177, 264, 297, 342, 526, 667
SfaNI GCATC 1 cut(s) 625
Sfr274I CTCGAG 1 cut(s) 245
Sfr303I CCGCGG 1 cut(s) 226
SfuI TTCGAA 1 cut(s) 373
SgrBI CCGCGG 1 cut(s) 226
SinI GGWCC 3 cut(s) 172, 220, 360
SlaI CTCGAG 1 cut(s) 245
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
Sse9I AATT 2 cut(s) 354, 507
SsiI CCGC 6 cut(s) 207, 223, 225, 366, 424, 482
SspMI CTAG 1 cut(s) 176
StyD4I CCNGG 3 cut(s) 32, 216, 499
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 2 cut(s) 82, 649
TaiI ACGT 2 cut(s) 141, 264
TaqI TCGA 4 cut(s) 246, 258, 373, 571
TaqII GACCGA 1 cut(s) 413
TasI AATT 2 cut(s) 354, 507
TauI GCSGC 1 cut(s) 369
TscAI CASTG 2 cut(s) 443, 553
TseFI GTSAC 2 cut(s) 139, 340
Tsp45I GTSAC 2 cut(s) 139, 340
TspGWI ACGGA 3 cut(s) 267, 297, 425
TspRI CASTG 2 cut(s) 443, 553
Van91I CCANNNNNTGG 1 cut(s) 560
VpaK11BI GGWCC 3 cut(s) 172, 220, 360
XcmI CCANNNNNNNNNTGG 1 cut(s) 427
XhoI CTCGAG 1 cut(s) 245
XmiI GTMKAC 1 cut(s) 258
XspI CTAG 1 cut(s) 176
ZraI GACGTC 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.