pycom06g18740

Single-stranded DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0014454
Physical Location & Seq
Reverse (-)
22361898 .. 22363686
1789 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g18740.2

Sequence Viewer

Length: 798 bp
ATGCAAATTAGCCCGAAAGGGTTTAAGCTTGCCCCAACCAAAACCCCTCAAAAATCAAGAGAGGGAATTGAAATTTGCACCGCCGAAACCATTTTCCTCTCAACAAAAATGGCTTCCTCCTCCATGGCCACGCTCTCGAGGACGCTTTCTCGCTCTCTGCAAGCAAAGTCCCGGACTTTACTCTCCATGCGCTTCACCACCACCACCACTGGCGCCTCCTCCTCCAGCAGCGACAGCTCCGACTCGGACGAGTCCAACCCTCTCAACTCGGAGGCAGAGTCAGCCCCGGGCGACGCCCCCCCACAGCCGCCTCCGTCCGAGGAACCCAATCGGAGAAACTTCATGTATGATAACCCGCTCGAGAACGGCCTCGACGCCGGCATTTATAAGGCGATTCTGGTCGGGCAGGTGGGGCAGAGTCCACTGCAGAAGAAGCTGAGGAGCGGGACCACCGTCACCATGTTTTCGGTTGGAACCGGCGGGATTCGAAACAACCGGCGGCCGTTGGATAATGAGGACCCGGTGGAGTATGCGAACCGCTGCAACGTCCAGTGGCACCGGGTTTGTGTTTATCCGGACCGGCTTAGCATGATTGCCATGAAGAATGTTGTGCCTGGTTCGATTCTATATTTGGAAGGTAATCTGGAGACCAAAATCTTCACTGATCCGGTTAGTGGTCTTGTTCGACGAGTTAGAGAGATTGCTGTTCGCAGAAACGGTCGGCTTGTGTTTTTGGGTAAAGGTGATGGTGCCGAGCAATCACCAAAATGGGGGCTCAAACGTGTCGGCTACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03430 ko03440 map03030 map03430 map03440
Pfam Domains
Protein Families
SSB

Protein Analysis

266

Amino Acids

29.05

Weight (kDa)

9.56

Isoelectric Point (pI)

62.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014454)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18580
fragaria_vesca FvH4_5g12820 FvH4_5g12820
malus_domestica MD06G1209800.v1.1 MD14G1220600.v1.1
prunus_persica Prupe.5G215900_v2.0.a1
pyrus_communis pycom06g18740
rosa_chinensis RchiOBHm_Chr7g0184411
rosa_laevigata RLG00000004977
rosa_multiflora Rmu_co8454851.1_g000001 Rmu_sc0001557.1_g000018
rosa_roxburghii Rroxscaffold_3G00269650
rosa_samantha Rh7AG080200 Rh7BG067000 Rh7CG067300 Rh7DG067000
rosa_wichuraiana Rw7G005510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 387
AarI CACCTGC 1 cut(s) 397
Acc36I ACCTGC 1 cut(s) 397
AccB1I GGYRCC 3 cut(s) 212, 555, 749
AccBSI CCGCTC 2 cut(s) 358, 444
AccIII TCCGGA 1 cut(s) 574
AciI CCGC 7 cut(s) 81, 308, 356, 444, 480, 499, 538
AclWI GGATC 1 cut(s) 659
AcoI YGGCCR 2 cut(s) 126, 500
AcsI RAATTY 1 cut(s) 72
AcyI GRCGYC 3 cut(s) 213, 294, 375
AfiI CCNNNNNNNGG 2 cut(s) 674, 770
AflIII ACRYGT 1 cut(s) 781
AgsI TTSAA 1 cut(s) 71
AjnI CCWGG 1 cut(s) 613
AluBI AGCT 3 cut(s) 28, 237, 436
AluI AGCT 3 cut(s) 28, 237, 436
Alw26I GTCTC 1 cut(s) 641
AlwI GGATC 1 cut(s) 659
Ama87I CYCGRG 3 cut(s) 136, 286, 359
Aor13HI TCCGGA 1 cut(s) 574
AoxI GGCC 3 cut(s) 126, 367, 500
ApeKI GCWGC 2 cut(s) 228, 540
ApoI RAATTY 1 cut(s) 72
AspLEI GCGC 2 cut(s) 192, 215
AspS9I GGNCC 3 cut(s) 447, 517, 577
AsuC2I CCSGG 5 cut(s) 172, 287, 288, 521, 560
AsuHPI GGTGA 4 cut(s) 187, 448, 753, 755
AsuII TTCGAA 1 cut(s) 487
AvaI CYCGRG 3 cut(s) 136, 286, 359
AvaII GGWCC 3 cut(s) 447, 517, 577
BalI TGGCCA 1 cut(s) 128
BanI GGYRCC 3 cut(s) 212, 555, 749
BanII GRGCYC 1 cut(s) 777
BbvCI CCTCAGC 1 cut(s) 437
BbvI GCAGC 2 cut(s) 240, 527
BccI CCATC 1 cut(s) 740
BceAI ACGGC 2 cut(s) 382, 487
BciT130I CCWGG 1 cut(s) 615
BcnI CCSGG 5 cut(s) 172, 287, 288, 521, 560
BcoDI GTCTC 1 cut(s) 641
BfmI CTRYAG 1 cut(s) 425
BfoI RGCGCY 1 cut(s) 216
BfuAI ACCTGC 1 cut(s) 397
BisI GCNGC 4 cut(s) 229, 308, 500, 541
BlpI GCTNAGC 1 cut(s) 584
BlsI GCNGC 4 cut(s) 230, 309, 501, 542
Bme1390I CCNGG 6 cut(s) 172, 287, 288, 521, 560, 615
Bme18I GGWCC 3 cut(s) 447, 517, 577
BmeT110I CYCGRG 3 cut(s) 136, 286, 359
BmgT120I GGNCC 3 cut(s) 447, 517, 577
BmiI GGNNCC 7 cut(s) 214, 324, 448, 475, 519, 557, 751
BmrFI CCNGG 6 cut(s) 172, 287, 288, 521, 560, 615
BoxI GACNNNNGTC 1 cut(s) 452
BpmI CTGGAG 2 cut(s) 208, 665
Bpu10I CCTNAGC 1 cut(s) 437
Bpu1102I GCTNAGC 1 cut(s) 584
Bpu14I TTCGAA 1 cut(s) 487
BpuMI CCSGG 5 cut(s) 172, 287, 288, 521, 560
BsaHI GRCGYC 3 cut(s) 213, 294, 375
BsaI GGTCTC 1 cut(s) 641
BsaJI CCNNGG 4 cut(s) 123, 285, 286, 318
BsaWI WCCGGW 2 cut(s) 574, 667
BsaXI ACNNNNNCTCC 2 cut(s) 263, 293
Bsc4I CCNNNNNNNGG 2 cut(s) 674, 770
Bse118I RCCGGY 4 cut(s) 377, 476, 495, 579
Bse1I ACTGG 2 cut(s) 214, 550
BseAI TCCGGA 1 cut(s) 574
BseBI CCWGG 1 cut(s) 615
BseDI CCNNGG 4 cut(s) 123, 285, 286, 318
BseLI CCNNNNNNNGG 2 cut(s) 674, 770
BseMII CTCAG 1 cut(s) 428
BseNI ACTGG 2 cut(s) 214, 550
BseRI GAGGAG 4 cut(s) 109, 208, 211, 454
BseX3I CGGCCG 1 cut(s) 500
BseXI GCAGC 2 cut(s) 240, 527
Bsh1285I CGRYCG 2 cut(s) 503, 721
BshFI GGCC 3 cut(s) 128, 369, 502
BshNI GGYRCC 3 cut(s) 212, 555, 749
BsiEI CGRYCG 2 cut(s) 503, 721
BsiHKCI CYCGRG 3 cut(s) 136, 286, 359
BslFI GGGAC 2 cut(s) 154, 460
BslI CCNNNNNNNGG 2 cut(s) 674, 770
BsmAI GTCTC 1 cut(s) 641
BsmFI GGGAC 2 cut(s) 154, 460
BsnI GGCC 3 cut(s) 128, 369, 502
Bso31I GGTCTC 1 cut(s) 641
BsoBI CYCGRG 3 cut(s) 136, 286, 359
Bsp119I TTCGAA 1 cut(s) 487
Bsp1286I GDGCHC 1 cut(s) 777
Bsp13I TCCGGA 1 cut(s) 574
Bsp143I GATC 1 cut(s) 664
Bsp1720I GCTNAGC 1 cut(s) 584
Bsp19I CCATGG 1 cut(s) 123
BspACI CCGC 7 cut(s) 81, 308, 356, 444, 480, 499, 538
BspANI GGCC 3 cut(s) 128, 369, 502
BspCNI CTCAG 1 cut(s) 429
BspEI TCCGGA 1 cut(s) 574
BspLI GGNNCC 7 cut(s) 214, 324, 448, 475, 519, 557, 751
BspMAI CTGCAG 1 cut(s) 429
BspMI ACCTGC 1 cut(s) 397
BspPI GGATC 1 cut(s) 659
BspT104I TTCGAA 1 cut(s) 487
BspT107I GGYRCC 3 cut(s) 212, 555, 749
BspTNI GGTCTC 1 cut(s) 641
BsrBI CCGCTC 2 cut(s) 358, 444
BsrFI RCCGGY 4 cut(s) 377, 476, 495, 579
BsrI ACTGG 2 cut(s) 214, 550
BssAI RCCGGY 4 cut(s) 377, 476, 495, 579
BssECI CCNNGG 4 cut(s) 123, 285, 286, 318
BssMI GATC 1 cut(s) 664
BssNI GRCGYC 3 cut(s) 213, 294, 375
BssT1I CCWWGG 1 cut(s) 123
Bst2UI CCWGG 1 cut(s) 615
Bst4CI ACNGT 2 cut(s) 454, 719
BstACI GRCGYC 3 cut(s) 213, 294, 375
BstBI TTCGAA 1 cut(s) 487
BstC8I GCNNGC 3 cut(s) 30, 162, 379
BstDEI CTNAG 2 cut(s) 437, 584
BstDSI CCRYGG 1 cut(s) 123
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 2 cut(s) 192, 215
BstKTI GATC 1 cut(s) 667
BstMAI GTCTC 1 cut(s) 641
BstMBI GATC 1 cut(s) 664
BstMCI CGRYCG 2 cut(s) 503, 721
BstMWI GCNNNNNNNGC 4 cut(s) 234, 281, 412, 433
BstNI CCWGG 1 cut(s) 615
BstPAI GACNNNNGTC 1 cut(s) 452
BstSCI CCNGG 6 cut(s) 170, 285, 286, 519, 558, 613
BstSFI CTRYAG 1 cut(s) 425
BstV1I GCAGC 2 cut(s) 240, 527
BstZI CGGCCG 1 cut(s) 500
BsuRI GGCC 3 cut(s) 128, 369, 502
BtgI CCRYGG 1 cut(s) 123
BtsI GCAGTG 1 cut(s) 422
BtsIMutI CAGTG 4 cut(s) 207, 422, 557, 660
BveI ACCTGC 1 cut(s) 397
Cac8I GCNNGC 3 cut(s) 30, 162, 379
CfoI GCGC 2 cut(s) 192, 215
Cfr10I RCCGGY 4 cut(s) 377, 476, 495, 579
Cfr13I GGNCC 3 cut(s) 447, 517, 577
Cfr9I CCCGGG 1 cut(s) 286
CpoI CGGWCCG 1 cut(s) 577
CseI GACGC 3 cut(s) 151, 302, 383
CspI CGGWCCG 1 cut(s) 577
CviAII CATG 6 cut(s) 124, 187, 343, 460, 589, 598
DdeI CTNAG 2 cut(s) 437, 584
DinI GGCGCC 1 cut(s) 214
DpnI GATC 1 cut(s) 666
DpnII GATC 1 cut(s) 664
EaeI YGGCCR 2 cut(s) 126, 500
EagI CGGCCG 1 cut(s) 500
EclXI CGGCCG 1 cut(s) 500
Eco130I CCWWGG 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 777
Eco31I GGTCTC 1 cut(s) 641
Eco47I GGWCC 3 cut(s) 447, 517, 577
Eco52I CGGCCG 1 cut(s) 500
Eco88I CYCGRG 3 cut(s) 136, 286, 359
EcoO109I RGGNCCY 1 cut(s) 517
EcoRII CCWGG 1 cut(s) 613
EcoT14I CCWWGG 1 cut(s) 123
EcoT38I GRGCYC 1 cut(s) 777
EgeI GGCGCC 1 cut(s) 214
EheI GGCGCC 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 6 cut(s) 127, 190, 346, 463, 592, 601
FaqI GGGAC 2 cut(s) 154, 460
FatI CATG 6 cut(s) 123, 186, 342, 459, 588, 597
FauI CCCGC 3 cut(s) 363, 437, 473
Fnu4HI GCNGC 4 cut(s) 229, 308, 500, 541
FriOI GRGCYC 1 cut(s) 777
Fsp4HI GCNGC 4 cut(s) 229, 308, 500, 541
GlaI GCGC 2 cut(s) 191, 214
GluI GCNGC 4 cut(s) 229, 308, 500, 541
GsuI CTGGAG 2 cut(s) 208, 665
HaeII RGCGCY 1 cut(s) 216
HaeIII GGCC 3 cut(s) 128, 369, 502
HgaI GACGC 3 cut(s) 151, 302, 383
HhaI GCGC 2 cut(s) 192, 215
Hin1I GRCGYC 3 cut(s) 213, 294, 375
Hin1II CATG 6 cut(s) 127, 190, 346, 463, 592, 601
Hin6I GCGC 2 cut(s) 190, 213
HinP1I GCGC 2 cut(s) 190, 213
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 7 cut(s) 242, 251, 278, 394, 418, 484, 622
HphI GGTGA 4 cut(s) 187, 448, 753, 755
Hpy166II GTNNAC 1 cut(s) 422
Hpy188I TCNGA 5 cut(s) 241, 247, 271, 319, 333
Hpy188III TCNNGA 5 cut(s) 57, 136, 361, 575, 644
Hpy8I GTNNAC 1 cut(s) 422
Hpy99I CGWCG 3 cut(s) 296, 377, 690
HpyAV CCTTC 1 cut(s) 629
HpyCH4III ACNGT 2 cut(s) 454, 719
HpyCH4IV ACGT 2 cut(s) 546, 781
HpyCH4V TGCA 5 cut(s) 4, 78, 160, 427, 543
HpyF10VI GCNNNNNNNGC 4 cut(s) 234, 281, 412, 433
HpyF3I CTNAG 2 cut(s) 437, 584
HpySE526I ACGT 2 cut(s) 546, 781
Hsp92I GRCGYC 3 cut(s) 213, 294, 375
Hsp92II CATG 6 cut(s) 127, 190, 346, 463, 592, 601
HspAI GCGC 2 cut(s) 190, 213
KasI GGCGCC 1 cut(s) 212
Kpn2I TCCGGA 1 cut(s) 574
KroI GCCGGC 1 cut(s) 377
KroNI GCCGGC 1 cut(s) 379
Kzo9I GATC 1 cut(s) 664
LmnI GCTCC 2 cut(s) 242, 441
Lsp1109I GCAGC 2 cut(s) 240, 527
MaeII ACGT 2 cut(s) 546, 781
MaeIII GTNAC 1 cut(s) 454
MalI GATC 1 cut(s) 666
MbiI CCGCTC 2 cut(s) 358, 444
MboI GATC 1 cut(s) 664
MboII GAAGA 3 cut(s) 442, 613, 649
MhlI GDGCHC 1 cut(s) 777
MlsI TGGCCA 1 cut(s) 128
MluCI AATT 3 cut(s) 6, 66, 72
MluNI TGGCCA 1 cut(s) 128
Mly113I GGCGCC 1 cut(s) 213
MlyI GAGTC 4 cut(s) 236, 260, 287, 427
MmeI TCCRAC 4 cut(s) 264, 279, 451, 486
Mox20I TGGCCA 1 cut(s) 128
MroI TCCGGA 1 cut(s) 574
MroNI GCCGGC 1 cut(s) 377
MscI TGGCCA 1 cut(s) 128
MseI TTAA 1 cut(s) 24
MslI CAYNNNNRTG 1 cut(s) 766
Msp20I TGGCCA 1 cut(s) 128
MspA1I CMGCKG 1 cut(s) 540
MspR9I CCNGG 6 cut(s) 172, 287, 288, 521, 560, 615
MvaI CCWGG 1 cut(s) 615
MwoI GCNNNNNNNGC 4 cut(s) 234, 281, 412, 433
NaeI GCCGGC 1 cut(s) 379
NarI GGCGCC 1 cut(s) 213
NciI CCSGG 5 cut(s) 172, 287, 288, 521, 560
NcoI CCATGG 1 cut(s) 123
NdeII GATC 1 cut(s) 664
NgoMIV GCCGGC 1 cut(s) 377
NlaIII CATG 6 cut(s) 127, 190, 346, 463, 592, 601
NlaIV GGNNCC 7 cut(s) 214, 324, 448, 475, 519, 557, 751
NmeAIII GCCGAG 1 cut(s) 778
NmuCI GTSAC 1 cut(s) 454
NspV TTCGAA 1 cut(s) 487
PaeR7I CTCGAG 2 cut(s) 136, 359
PaqCI CACCTGC 1 cut(s) 397
PdiI GCCGGC 1 cut(s) 379
PfeI GAWTC 3 cut(s) 394, 484, 622
PfoI TCCNGGA 1 cut(s) 170
PkrI GCNGC 4 cut(s) 230, 309, 501, 542
PleI GAGTC 4 cut(s) 236, 259, 286, 426
PluTI GGCGCC 1 cut(s) 216
PpsI GAGTC 4 cut(s) 236, 259, 286, 426
PpuMI RGGWCCY 1 cut(s) 517
PshAI GACNNNNGTC 1 cut(s) 452
PsiI TTATAA 1 cut(s) 387
Psp5II RGGWCCY 1 cut(s) 517
Psp6I CCWGG 1 cut(s) 613
PspGI CCWGG 1 cut(s) 613
PspN4I GGNNCC 7 cut(s) 214, 324, 448, 475, 519, 557, 751
PspPI GGNCC 3 cut(s) 447, 517, 577
PspPPI RGGWCCY 1 cut(s) 517
PstI CTGCAG 1 cut(s) 429
RseI CAYNNNNRTG 1 cut(s) 766
Rsr2I CGGWCCG 1 cut(s) 577
RsrII CGGWCCG 1 cut(s) 577
SaqAI TTAA 1 cut(s) 24
SatI GCNGC 4 cut(s) 229, 308, 500, 541
Sau3AI GATC 1 cut(s) 664
Sau96I GGNCC 3 cut(s) 447, 517, 577
SchI GAGTC 4 cut(s) 236, 260, 287, 427
ScrFI CCNGG 6 cut(s) 172, 287, 288, 521, 560, 615
SduI GDGCHC 1 cut(s) 777
SetI ASST 8 cut(s) 30, 239, 411, 438, 549, 640, 745, 784
SfcI CTRYAG 1 cut(s) 425
SfoI GGCGCC 1 cut(s) 214
Sfr274I CTCGAG 2 cut(s) 136, 359
SfuI TTCGAA 1 cut(s) 487
SinI GGWCC 3 cut(s) 447, 517, 577
SlaI CTCGAG 2 cut(s) 136, 359
SmaI CCCGGG 1 cut(s) 288
SmiMI CAYNNNNRTG 1 cut(s) 766
SmlI CTYRAG 2 cut(s) 136, 359
SmoI CTYRAG 2 cut(s) 136, 359
Sse9I AATT 3 cut(s) 6, 66, 72
SsiI CCGC 7 cut(s) 81, 308, 356, 444, 480, 499, 538
SspDI GGCGCC 1 cut(s) 212
StyD4I CCNGG 6 cut(s) 170, 285, 286, 519, 558, 613
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 2 cut(s) 454, 719
TaiI ACGT 2 cut(s) 549, 784
TaqI TCGA 6 cut(s) 137, 360, 372, 487, 620, 685
TasI AATT 3 cut(s) 6, 66, 72
TauI GCSGC 2 cut(s) 310, 502
TfiI GAWTC 3 cut(s) 394, 484, 622
Tru1I TTAA 1 cut(s) 24
Tru9I TTAA 1 cut(s) 24
TscAI CASTG 4 cut(s) 214, 429, 557, 667
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 2 cut(s) 228, 540
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 2 cut(s) 331, 614
TspGWI ACGGA 1 cut(s) 303
TspMI CCCGGG 1 cut(s) 286
TspRI CASTG 4 cut(s) 214, 429, 557, 667
VpaK11BI GGWCC 3 cut(s) 447, 517, 577
XapI RAATTY 1 cut(s) 72
XhoI CTCGAG 2 cut(s) 136, 359
XmaI CCCGGG 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.