FvH4_5g13410

ATP-dependent helicase hrq1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006845
Physical Location & Seq
Forward (+)
7572498 .. 7580232
7735 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g13410.t2

Sequence Viewer

Length: 3606 bp
ATGGCAGCCAGCCGGATTCAAATCACGGTCCGCACGGTGACCGGCGAAGCCACCACCGTCTCAGCCTCCGTCAACGACACCGTTTCAGACCTCAAGCTACTCCTCAACCACTCCTTCCCTCCCGCCACCACCAACTCCCCCAATTTCCACCTCTTCTTCAAGGGTGAGAAGTTGAAGTTGCAGAGTCGAATAGGTGCACTTAGTATTGAGCCCGGTGAGTTTCTAGTGCTGGTCCCGTTTGTTAAGAAGGTTGTTAATAATCGGAGCCAGAAATGTGATCAATCTGAGCCGTTTTCGGTGGCTACTTCTGCTAAAGGCTCCATGTCCAAGTTTGCGAACTCCGTGTGGAGCGATATGATGCAGGACTTGTCGTATTTACGCGATGATTTGAGTAATGGAACGGCGAGTGTGTCTAGTATAGGGAGTTTTGATGTTGGAGGTGGAAGTGAGGATTGCTCTCGTGAAGCGAAGCGGAAAAGGGGCGTCGGTTGTGATGACATGATATTGGATATGTTGCGAGCTAGTAGAAGCAAGAGTGTTCTTGATGAGCAGAATTTTAGAAGGATTGTTGAGCTTTTGGAGTCGGTGAATTGTTTATCGGACCCATATTCTGGGGACTGCATGTTGTGGAGAAGAAAAGCTAGCTTACAAGGCTGTGGCTTGGGGCTGCATAATGGCAGTGGTAATTCATGCTTGTGTCCGGATTGGTTGAAGAAAATCATGAAGGCTTTTGCTTTCTTAAACAATTTTTCTGCACTGGTTCAACTTCAGCAGGAGAGGACAACCTCAACTCTAGTGGAGCAAGCACTAGAACAGCTTGCGACATTTGGAGTTAAACTTGGTATGCAGGATGTGGAGAATCTTTCGTTTATTTCTCCCAAGGTAGTTCTTTTTTTAAAGGAGAATGCAGAGGCAACTTTTGGTAATGCACTTGTTATTACTGAATGTTCAGTAGAACAGAACCATGGTGACAGAAATAGTCATGAATCTGATTGCAAACAGATGGATGTATCAAAGATTGTCAATGAATTGAAGATGCATGAAAGTTCTTTCAAAAGTAATTTGTGGGAGGCTGTTGAGAGCCTAATGTTTATCAAAAGAAATGAGATTTCCTCCTTGGAAGACTTTCTCATTTATGTGAAGGAATGTGGTGCTGCTGCAAGAGGAAATAAAGTGAATCAAGCAATGAGAAGCCACACTGCTTCAACTACTGATTCAGACAAAAATCAATCTGCGGCTTCAACCACTCGTTCCACCAAAAAACGGGCAGGATGTCATAGAACCGATCCATTGCTACCAGTTGAGATGGTTGAACATCTTAGAAAGAGTTTTCGAGCAGAGAAGATAGTGCATGTTGAAGACATTGGTGCTCGGAAAGCTGTTCATGTGGAAGTTCCACAGGAACTAACAGAAAATATGAGATCTGCACTTCATTCTGTTGGAATCACTCAGTTATATAGTCATCAGGCAGAGTCAATACAAGCCTCAGTAACAGGTAAGAATGTCGTTGTGGCTACAATGACATCCAGTGGGAAATCTCTTTGCTACAATCTGCCTGTCATTGAAGCCCTATCCCAGAGTTCCTCAGCATGTGCTCTATACTTATTTCCAACAAAGGCTTTAGCTCAAGATCAACTAAGAGCTTTCTCAGCCATGAGCAAAGGATTCTGTGGTAGCTTAAATGTTGGTATATATGATGGTGACACTAATCAGCAGGAAAGGACACGACTGCGTCAAAATGCTAGACTGCTAATCACAAATCCAGATATGTTGCATGTATCAATCCTGCCGCACCATCGAAAATTTGGCCGGATTTTGGCAAATCTTAGGTATGTAGTGATTGATGAGGCCCATGTATATAAGGGAGCATTTGGTTGTCATACTGCTCTTATACTTAGACGACTTCGCCGGCTCTGTTCACATGTGTATGGAAGTACTCCTTCTTTTGTATTTTCTACTGCGACTTCGGCAAATCCTCGTGAGCATTGCATGGAACTTGCAAATTTACCAACTTTAGAGTTGATTCAGAATGATGGAAGCCCTGCTGCTAGAAAGCTTTTCATCCTTTGGAATTCTGATGGCAGCCCGATTGAGGATATTTCAGTCCTCTTTGCTGAAATGGTGCAGCATGGACTACGGTGTATTGCGTTTTGCAAAACTCGCAAGCTTTGTGAACTTGTTTTTTGCAATACGCGTGAAATTCTTCAGCAGACAGCACCCCATCTGGCAGACTCTATATCATCTTATCGTGGCGGTTACATGGCTGAGGATCGGAGAAGAATAGAGAGCGATTTATTTAATGGAAAGCTTTGTGGTATCGCTGCAACAAATGCCCTTGAATTGGGTATTGATGTTGGACACATTGATGTAACTCTTCATTTAGGTTTTCCTGGAAGCATTGCTAGCTTGTGGCAACAAGCTGGCAGATCCGGAAGGAGAGAGAGACCTTCTCTTGCTGTATATGTTGCATTTGAAGGGCCCCTTGACCAATATTTTATGAAACATCCAAATAAACTTTTTGGAAGCCCAATTGAGTGCTGTCATGTTGATGCTAAGAACCAACAGGTTCTTGAACAGCATTTGGCTTGTGCTGCTCTCGAACACCCACTGAGCTTGTTTCATGATGAGAAGTTTTTCGGTTCTGGTTTAGACAGAGGCATAATGTCTCTTAAAAGTACAGGCTATCTGAGGCTATCTGAGAACAGCATTTGGAACTATATTGGGCGCGAGAAAATGCCTTCACATTCGGTTAGTATACGATCAATTGAAAGAGAGAAGTACAAAGTTATGGACCATCAAGAGAACGAAATCGAAGAGATTGAGGAAAGCAAGGCTTTCTTTCAGGTATATGAAGGTGCTGTTTATTTGAACCAAGGAAAGACATACCTGGTCACGAGCTTAGATTTAAAAGAAAAGATTGCTTGGTGTGAAGAATCTAATTTAAATTTTTACACGAAAACTCGTGATCGCACAGATATTGATGTAGTCGGCTGTAATGTGGCTTATTCAGCTCCACCTAAGCTTTCCAATCAGTTCTCTAGAACAACTGCCCGAGCAGATCCTTGCAGAGTAACAACTACATGGTTTGGTTTCTATCGTATACAGAAAGGATGCAACACAATATTAGATAAAGTTGACCTTCAACTTCCTAAATATTCGTATGCATCACAGGCAGTTTGGGTTCCGGTTCCGCCATCAATAATTGAAGCAGTGAAGAGTGAAAATCTTGAATACCGTGCAGGGTTGCATGCTGCATCACATGCTGTTCTTCACGTTGTGCCTTTACGAATCATTTGTAACTTATCCGACTTGGCTCCAGAGTGTGCGGATCCTCATGAAAGCAGCTATTTTCCGGAGAGGATTCTGTTATATGATAAGCATCCTGGAGGGACTGGTATCTGTGTACAGATTCAGCCAATATTTATGGAGCTCTTGGCTACTGCTCTGGAACTTCTAACATCTTGCCACTGCGGAGAATATGATGGTTGCCCCAATTGCGTTCAGAGTTTTTGTTGCAGGTCATATAACGAGGTTTTACACAAAGATGCAGCAATTATGATCATCAAGGGTGTTCTGGACGCAAATAAATCTCCCAGCTCTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DUF1998 Helicase_C

Protein Analysis

1202

Amino Acids

133.39

Weight (kDa)

6.96

Isoelectric Point (pI)

47.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 484 - 621 1.2e-06 Type III restriction enzyme, res subunit
DEAD PF00270 487 - 657 2.3e-30 DEAD/DEAH box helicase
Helicase_C PF00271 708 - 813 9.3e-13 Helicase conserved C-terminal domain
WHD_HRQ1 PF22982 849 - 922 4.4e-16 ATP-dependent helicase HRQ1, winged helix domain
MZB PF09369 1085 - 1134 1.4e-06 MrfA Zn-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 3507
AccB7I CCANNNNNTGG 1 cut(s) 611
AccI GTMKAC 2 cut(s) 2755, 3100
AccII CGCG 3 cut(s) 381, 2194, 2727
AccIII TCCGGA 3 cut(s) 700, 2429, 3352
AciI CCGC 9 cut(s) 31, 123, 472, 1235, 1790, 2253, 3191, 3326, 3471
AclWI GGATC 6 cut(s) 1280, 2277, 2421, 3053, 3323, 3336
AcoI YGGCCR 1 cut(s) 1807
AcsI RAATTY 6 cut(s) 553, 1802, 2002, 2071, 2199, 2944
AcuI CTGAAG 2 cut(s) 752, 2189
AcyI GRCGYC 1 cut(s) 483
AdeI CACNNNGTG 1 cut(s) 3277
AfaI GTAC 4 cut(s) 1936, 2677, 2780, 3405
AfiI CCNNNNNNNGG 5 cut(s) 611, 1263, 1816, 2092, 2341
AflIII ACRYGT 2 cut(s) 1921, 2192
AjnI CCWGG 3 cut(s) 2389, 2886, 3382
Alw21I GWGCWC 4 cut(s) 199, 1372, 1597, 3432
Alw26I GTCTC 3 cut(s) 64, 2437, 2670
Alw44I GTGCAC 1 cut(s) 195
AlwI GGATC 6 cut(s) 1280, 2277, 2421, 3053, 3323, 3336
Ama87I CYCGRG 1 cut(s) 3051
Aor13HI TCCGGA 3 cut(s) 700, 2429, 3352
AoxI GGCC 3 cut(s) 1807, 1848, 2477
ApaI GGGCCC 1 cut(s) 2481
ApaLI GTGCAC 1 cut(s) 195
ApoI RAATTY 6 cut(s) 553, 1802, 2002, 2071, 2199, 2944
Asp700I GAANNNNTTC 3 cut(s) 1125, 2202, 2633
AspLEI GCGC 1 cut(s) 2727
AspS9I GGNCC 7 cut(s) 28, 232, 601, 1849, 2477, 2478, 2791
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 6 cut(s) 49, 176, 227, 598, 980, 1712
AsuNHI GCTAGC 2 cut(s) 641, 2402
AvaI CYCGRG 1 cut(s) 3051
AvaII GGWCC 4 cut(s) 28, 232, 601, 2791
BaeGI GKGCMC 2 cut(s) 199, 2481
BamHI GGATCC 1 cut(s) 3328
BanII GRGCYC 3 cut(s) 213, 2481, 3432
BauI CACGAG 4 cut(s) 459, 1977, 2893, 2961
BbsI GAAGAC 2 cut(s) 1128, 1365
Bbv12I GWGCWC 4 cut(s) 199, 1372, 1597, 3432
BbvCI CCTCAGC 2 cut(s) 1585, 2265
BceAI ACGGC 2 cut(s) 274, 417
BciT130I CCWGG 3 cut(s) 2391, 2888, 3384
BclI TGATCA 2 cut(s) 277, 3558
BcnI CCSGG 1 cut(s) 213
BcoDI GTCTC 3 cut(s) 64, 2437, 2670
BfuAI ACCTGC 1 cut(s) 3507
BglII AGATCT 1 cut(s) 1421
BmcAI AGTACT 1 cut(s) 1936
Bme1390I CCNGG 4 cut(s) 213, 2391, 2888, 3384
Bme18I GGWCC 4 cut(s) 28, 232, 601, 2791
BmeT110I CYCGRG 1 cut(s) 3051
BmgT120I GGNCC 7 cut(s) 28, 232, 601, 1849, 2477, 2478, 2791
BmrFI CCNGG 4 cut(s) 213, 2391, 2888, 3384
BmsI GCATC 8 cut(s) 348, 1026, 2539, 3101, 3173, 3263, 3388, 3535
BmtI GCTAGC 2 cut(s) 645, 2406
BpiI GAAGAC 2 cut(s) 1128, 1365
BplI GAGNNNNNCTC 6 cut(s) 440, 472, 1097, 1129, 2434, 2466
BpmI CTGGAG 2 cut(s) 3300, 3405
Bpu10I CCTNAGC 3 cut(s) 1585, 2265, 3018
BpuEI CTTGAG 2 cut(s) 77, 1611
BpuMI CCSGG 1 cut(s) 213
BsaBI GATNNNNATC 2 cut(s) 20, 3378
BsaHI GRCGYC 1 cut(s) 483
BsaI GGTCTC 1 cut(s) 2437
BsaJI CCNNGG 4 cut(s) 879, 964, 1116, 2872
BsaWI WCCGGW 4 cut(s) 700, 2429, 3184, 3352
Bsc4I CCNNNNNNNGG 5 cut(s) 611, 1263, 1816, 2092, 2341
Bse118I RCCGGY 2 cut(s) 41, 1908
Bse1I ACTGG 4 cut(s) 762, 1298, 1527, 3397
Bse3DI GCAATG 4 cut(s) 1191, 1289, 1984, 2397
Bse8I GATNNNNATC 2 cut(s) 20, 3378
BseAI TCCGGA 3 cut(s) 700, 2429, 3352
BseBI CCWGG 3 cut(s) 2391, 2888, 3384
BseDI CCNNGG 4 cut(s) 879, 964, 1116, 2872
BseGI GGATG 8 cut(s) 856, 1012, 1277, 1523, 2061, 2503, 3116, 3379
BseJI GATNNNNATC 2 cut(s) 20, 3378
BseLI CCNNNNNNNGG 5 cut(s) 611, 1263, 1816, 2092, 2341
BseMI GCAATG 4 cut(s) 1191, 1289, 1984, 2397
BseNI ACTGG 4 cut(s) 762, 1298, 1527, 3397
BseRI GAGGAG 1 cut(s) 92
BseSI GKGCMC 2 cut(s) 199, 2481
BseYI CCCAGC 1 cut(s) 3593
BsgI GTGCAG 4 cut(s) 738, 1410, 2144, 3258
Bsh1236I CGCG 3 cut(s) 381, 2194, 2727
BshFI GGCC 3 cut(s) 1809, 1850, 2479
BsiHKAI GWGCWC 4 cut(s) 199, 1372, 1597, 3432
BsiHKCI CYCGRG 1 cut(s) 3051
BsiSI CCGG 9 cut(s) 13, 42, 213, 701, 1810, 1909, 2430, 3185, 3353
BslFI GGGAC 3 cut(s) 218, 629, 3403
BslI CCNNNNNNNGG 5 cut(s) 611, 1263, 1816, 2092, 2341
BsmAI GTCTC 3 cut(s) 64, 2437, 2670
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 3 cut(s) 218, 629, 3403
BsmI GAATGC 1 cut(s) 910
BsnI GGCC 3 cut(s) 1809, 1850, 2479
Bso31I GGTCTC 1 cut(s) 2437
BsoBI CYCGRG 1 cut(s) 3051
Bsp120I GGGCCC 1 cut(s) 2477
Bsp1286I GDGCHC 6 cut(s) 199, 213, 1372, 1597, 2481, 3432
Bsp13I TCCGGA 3 cut(s) 700, 2429, 3352
Bsp1407I TGTACA 1 cut(s) 3403
Bsp19I CCATGG 1 cut(s) 964
BspACI CCGC 9 cut(s) 31, 123, 472, 1235, 1790, 2253, 3191, 3326, 3471
BspANI GGCC 3 cut(s) 1809, 1850, 2479
BspEI TCCGGA 3 cut(s) 700, 2429, 3352
BspFNI CGCG 3 cut(s) 381, 2194, 2727
BspHI TCATGA 4 cut(s) 720, 982, 2620, 3334
BspMI ACCTGC 1 cut(s) 3507
BspOI GCTAGC 2 cut(s) 645, 2406
BspPI GGATC 6 cut(s) 1280, 2277, 2421, 3053, 3323, 3336
BspTNI GGTCTC 1 cut(s) 2437
BsrDI GCAATG 4 cut(s) 1191, 1289, 1984, 2397
BsrFI RCCGGY 2 cut(s) 41, 1908
BsrGI TGTACA 1 cut(s) 3403
BsrI ACTGG 4 cut(s) 762, 1298, 1527, 3397
BssAI RCCGGY 2 cut(s) 41, 1908
BssECI CCNNGG 4 cut(s) 879, 964, 1116, 2872
BssNAI GTATAC 2 cut(s) 2756, 3101
BssNI GRCGYC 1 cut(s) 483
BssSI CACGAG 4 cut(s) 459, 1977, 2893, 2961
BssT1I CCWWGG 4 cut(s) 879, 964, 1116, 2872
Bst1107I GTATAC 2 cut(s) 2756, 3101
Bst2BI CACGAG 4 cut(s) 459, 1977, 2893, 2961
Bst2UI CCWGG 3 cut(s) 2391, 2888, 3384
Bst4CI ACNGT 6 cut(s) 28, 37, 58, 82, 2139, 3236
Bst6I CTCTTC 4 cut(s) 158, 2379, 2808, 3209
BstACI GRCGYC 1 cut(s) 483
BstAPI GCANNNNNTGC 2 cut(s) 2330, 3260
BstAUI TGTACA 1 cut(s) 3403
BstDSI CCRYGG 1 cut(s) 964
BstEII GGTNACC 1 cut(s) 37
BstF5I GGATG 8 cut(s) 856, 1012, 1277, 1523, 2061, 2503, 3116, 3379
BstFNI CGCG 3 cut(s) 381, 2194, 2727
BstHHI GCGC 1 cut(s) 2727
BstMAI GTCTC 3 cut(s) 64, 2437, 2670
BstNI CCWGG 3 cut(s) 2391, 2888, 3384
BstNSI RCATGY 7 cut(s) 625, 1355, 1593, 1778, 1925, 3251, 3263
BstPI GGTNACC 1 cut(s) 37
BstSCI CCNGG 4 cut(s) 211, 2389, 2886, 3382
BstSLI GKGCMC 2 cut(s) 199, 2481
BstUI CGCG 3 cut(s) 381, 2194, 2727
BstV2I GAAGAC 2 cut(s) 1128, 1365
BstX2I RGATCY 4 cut(s) 1421, 2426, 3058, 3328
BstYI RGATCY 4 cut(s) 1421, 2426, 3058, 3328
BstZ17I GTATAC 2 cut(s) 2756, 3101
BsuRI GGCC 3 cut(s) 1809, 1850, 2479
BtgI CCRYGG 1 cut(s) 964
BtgZI GCGATG 1 cut(s) 396
BtsCI GGATG 8 cut(s) 856, 1012, 1277, 1523, 2061, 2503, 3116, 3379
BtsI GCAGTG 4 cut(s) 685, 1197, 3216, 3466
BtsIMutI CAGTG 7 cut(s) 685, 755, 1197, 1534, 2606, 3216, 3466
BveI ACCTGC 1 cut(s) 3507
CciI TCATGA 4 cut(s) 720, 982, 2620, 3334
CfoI GCGC 1 cut(s) 2727
Cfr10I RCCGGY 2 cut(s) 41, 1908
Cfr13I GGNCC 7 cut(s) 28, 232, 601, 1849, 2477, 2478, 2791
CpoI CGGWCCG 1 cut(s) 28
CseI GACGC 3 cut(s) 472, 1721, 3587
CsiI ACCWGGT 1 cut(s) 2886
Csp6I GTAC 4 cut(s) 1935, 2676, 2779, 3404
CspCI CAANNNNNGTGG 4 cut(s) 777, 812, 2595, 2630
CspI CGGWCCG 1 cut(s) 28
CviQI GTAC 4 cut(s) 1935, 2676, 2779, 3404
DraI TTTAAA 3 cut(s) 897, 2907, 2943
DraIII CACNNNGTG 1 cut(s) 3277
EaeI YGGCCR 1 cut(s) 1807
Eam1104I CTCTTC 4 cut(s) 158, 2379, 2808, 3209
EarI CTCTTC 4 cut(s) 158, 2379, 2808, 3209
EciI GGCGGA 1 cut(s) 3180
Ecl136II GAGCTC 1 cut(s) 3430
Eco130I CCWWGG 4 cut(s) 879, 964, 1116, 2872
Eco24I GRGCYC 3 cut(s) 213, 2481, 3432
Eco31I GGTCTC 1 cut(s) 2437
Eco47I GGWCC 4 cut(s) 28, 232, 601, 2791
Eco53kI GAGCTC 1 cut(s) 3430
Eco57I CTGAAG 2 cut(s) 752, 2189
Eco88I CYCGRG 1 cut(s) 3051
Eco91I GGTNACC 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 3430
EcoO109I RGGNCCY 2 cut(s) 2477, 2478
EcoO65I GGTNACC 1 cut(s) 37
EcoRI GAATTC 1 cut(s) 2071
EcoRII CCWGG 3 cut(s) 2389, 2886, 3382
EcoT14I CCWWGG 4 cut(s) 879, 964, 1116, 2872
EcoT22I ATGCAT 2 cut(s) 1041, 3166
EcoT38I GRGCYC 3 cut(s) 213, 2481, 3432
ErhI CCWWGG 4 cut(s) 879, 964, 1116, 2872
Esp3I CGTCTC 1 cut(s) 64
FaqI GGGAC 3 cut(s) 218, 629, 3403
FauI CCCGC 1 cut(s) 130
FbaI TGATCA 2 cut(s) 277, 3558
FblI GTMKAC 2 cut(s) 2755, 3100
FokI GGATG 8 cut(s) 863, 1019, 1284, 1510, 2048, 2490, 3123, 3366
FriOI GRGCYC 3 cut(s) 213, 2481, 3432
GlaI GCGC 1 cut(s) 2726
GsaI CCCAGC 1 cut(s) 3597
GsuI CTGGAG 2 cut(s) 3300, 3405
HaeIII GGCC 3 cut(s) 1809, 1850, 2479
HapII CCGG 9 cut(s) 13, 42, 213, 701, 1810, 1909, 2430, 3185, 3353
HgaI GACGC 3 cut(s) 472, 1721, 3587
HhaI GCGC 1 cut(s) 2727
Hin1I GRCGYC 1 cut(s) 483
Hin6I GCGC 1 cut(s) 2725
HinP1I GCGC 1 cut(s) 2725
HincII GTYRAC 2 cut(s) 73, 3136
HindII GTYRAC 2 cut(s) 73, 3136
HindIII AAGCTT 4 cut(s) 2054, 2165, 2306, 3020
HpaII CCGG 9 cut(s) 13, 42, 213, 701, 1810, 1909, 2430, 3185, 3353
HphI GGTGA 6 cut(s) 49, 176, 227, 598, 980, 1712
Hpy166II GTNNAC 8 cut(s) 73, 197, 1919, 2174, 2756, 3101, 3136, 3404
Hpy8I GTNNAC 8 cut(s) 73, 197, 1919, 2174, 2756, 3101, 3136, 3404
Hpy99I CGWCG 1 cut(s) 488
HpyCH4III ACNGT 6 cut(s) 28, 37, 58, 82, 2139, 3236
HpyCH4IV ACGT 1 cut(s) 3273
HpySE526I ACGT 1 cut(s) 3273
Hsp92I GRCGYC 1 cut(s) 483
HspAI GCGC 1 cut(s) 2725
Kpn2I TCCGGA 3 cut(s) 700, 2429, 3352
KroI GCCGGC 1 cut(s) 1908
KroNI GCCGGC 1 cut(s) 1910
Ksp22I TGATCA 2 cut(s) 277, 3558
LmnI GCTCC 8 cut(s) 264, 323, 348, 799, 1865, 3016, 3319, 3427
LweI GCATC 8 cut(s) 348, 1026, 2539, 3101, 3173, 3263, 3388, 3535
MabI ACCWGGT 1 cut(s) 2886
MaeII ACGT 1 cut(s) 3273
MaeIII GTNAC 9 cut(s) 37, 968, 1489, 1700, 2255, 2368, 2890, 3070, 3296
MfeI CAATTG 3 cut(s) 2529, 2763, 3493
MflI RGATCY 4 cut(s) 1421, 2426, 3058, 3328
MhlI GDGCHC 6 cut(s) 199, 213, 1372, 1597, 2481, 3432
MluI ACGCGT 1 cut(s) 2192
MlyI GAGTC 4 cut(s) 193, 590, 1481, 2225
MmeI TCCRAC 5 cut(s) 415, 1420, 1634, 2335, 3330
Mph1103I ATGCAT 2 cut(s) 1041, 3166
MroI TCCGGA 3 cut(s) 700, 2429, 3352
MroNI GCCGGC 1 cut(s) 1908
MroXI GAANNNNTTC 3 cut(s) 1125, 2202, 2633
MslI CAYNNNNRTG 6 cut(s) 694, 1136, 1926, 2364, 2547, 3543
MspI CCGG 9 cut(s) 13, 42, 213, 701, 1810, 1909, 2430, 3185, 3353
MspR9I CCNGG 4 cut(s) 213, 2391, 2888, 3384
MunI CAATTG 3 cut(s) 2529, 2763, 3493
Mva1269I GAATGC 1 cut(s) 910
MvaI CCWGG 3 cut(s) 2391, 2888, 3384
MvnI CGCG 3 cut(s) 381, 2194, 2727
NaeI GCCGGC 1 cut(s) 1910
NciI CCSGG 1 cut(s) 213
NcoI CCATGG 1 cut(s) 964
NgoMIV GCCGGC 1 cut(s) 1908
NheI GCTAGC 2 cut(s) 641, 2402
NmuCI GTSAC 4 cut(s) 37, 968, 1700, 2890
NsiI ATGCAT 2 cut(s) 1041, 3166
NspI RCATGY 7 cut(s) 625, 1355, 1593, 1778, 1925, 3251, 3263
PaeI GCATGC 1 cut(s) 3251
PagI TCATGA 4 cut(s) 720, 982, 2620, 3334
PciI ACATGT 1 cut(s) 1921
PctI GAATGC 1 cut(s) 910
PdiI GCCGGC 1 cut(s) 1910
PdmI GAANNNNTTC 3 cut(s) 1125, 2202, 2633
PflFI GACNNNGTC 1 cut(s) 1731
PflMI CCANNNNNTGG 1 cut(s) 611
PfoI TCCNGGA 2 cut(s) 2389, 3382
PleI GAGTC 4 cut(s) 192, 589, 1480, 2225
PpsI GAGTC 4 cut(s) 192, 589, 1480, 2225
PscI ACATGT 1 cut(s) 1921
Psp124BI GAGCTC 1 cut(s) 3432
Psp6I CCWGG 3 cut(s) 2389, 2886, 3382
PspEI GGTNACC 1 cut(s) 37
PspFI CCCAGC 1 cut(s) 3593
PspGI CCWGG 3 cut(s) 2389, 2886, 3382
PspOMI GGGCCC 1 cut(s) 2477
PspPI GGNCC 7 cut(s) 28, 232, 601, 1849, 2477, 2478, 2791
PsuI RGATCY 4 cut(s) 1421, 2426, 3058, 3328
PsyI GACNNNGTC 1 cut(s) 1731
RsaI GTAC 4 cut(s) 1936, 2677, 2780, 3405
RsaNI GTAC 4 cut(s) 1935, 2676, 2779, 3404
RseI CAYNNNNRTG 6 cut(s) 694, 1136, 1926, 2364, 2547, 3543
Rsr2I CGGWCCG 1 cut(s) 28
RsrII CGGWCCG 1 cut(s) 28
SacI GAGCTC 1 cut(s) 3432
Sau96I GGNCC 7 cut(s) 28, 232, 601, 1849, 2477, 2478, 2791
ScaI AGTACT 1 cut(s) 1936
SchI GAGTC 4 cut(s) 193, 590, 1481, 2225
ScrFI CCNGG 4 cut(s) 213, 2391, 2888, 3384
SduI GDGCHC 6 cut(s) 199, 213, 1372, 1597, 2481, 3432
SexAI ACCWGGT 1 cut(s) 2886
SfaNI GCATC 8 cut(s) 348, 1026, 2539, 3101, 3173, 3263, 3388, 3535
SinI GGWCC 4 cut(s) 28, 232, 601, 2791
SmiI ATTTAAAT 1 cut(s) 2943
SmiMI CAYNNNNRTG 6 cut(s) 694, 1136, 1926, 2364, 2547, 3543
SmlI CTYRAG 2 cut(s) 92, 1626
SmoI CTYRAG 2 cut(s) 92, 1626
SphI GCATGC 1 cut(s) 3251
SsiI CCGC 9 cut(s) 31, 123, 472, 1235, 1790, 2253, 3191, 3326, 3471
SspI AATATT 4 cut(s) 2492, 3123, 3155, 3420
SstI GAGCTC 1 cut(s) 3432
StyD4I CCNGG 4 cut(s) 211, 2389, 2886, 3382
StyI CCWWGG 4 cut(s) 879, 964, 1116, 2872
SwaI ATTTAAAT 1 cut(s) 2943
TaaI ACNGT 6 cut(s) 28, 37, 58, 82, 2139, 3236
TaiI ACGT 1 cut(s) 3276
TaqI TCGA 5 cut(s) 187, 1333, 1798, 2598, 2811
TatI WGTACW 4 cut(s) 1934, 2675, 2778, 3403
TauI GCSGC 2 cut(s) 1238, 1792
TscAI CASTG 7 cut(s) 685, 762, 1204, 1534, 2613, 3216, 3473
TseFI GTSAC 4 cut(s) 37, 968, 1700, 2890
Tsp45I GTSAC 4 cut(s) 37, 968, 1700, 2890
TspGWI ACGGA 2 cut(s) 58, 331
TspRI CASTG 7 cut(s) 685, 762, 1204, 1534, 2613, 3216, 3473
Tth111I GACNNNGTC 1 cut(s) 1731
Van91I CCANNNNNTGG 1 cut(s) 611
VneI GTGCAC 1 cut(s) 195
VpaK11BI GGWCC 4 cut(s) 28, 232, 601, 2791
XapI RAATTY 6 cut(s) 553, 1802, 2002, 2071, 2199, 2944
XbaI TCTAGA 1 cut(s) 3038
XceI RCATGY 7 cut(s) 625, 1355, 1593, 1778, 1925, 3251, 3263
XcmI CCANNNNNNNNNTGG 1 cut(s) 1802
XmiI GTMKAC 2 cut(s) 2755, 3100
XmnI GAANNNNTTC 3 cut(s) 1125, 2202, 2633
ZrmI AGTACT 1 cut(s) 1936
Zsp2I ATGCAT 2 cut(s) 1041, 3166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.