RLG00000005031

ATP-dependent helicase hrq1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006845
Physical Location & Seq
Forward (+)
64447806 .. 64455643
7838 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005031

Sequence Viewer

Length: 3627 bp
ATGGCAGCGAGTCGGATTCAAATCGAGGTCCGTACATTGACCGGCGAAGCGACCACCGTCTCAGCCTCCGTCAACGACGCCGTTTCAGACCTCAAGCTACTTCTCAACCAGTCCTTCCCTCCCGCCATCAAATCTCCCAATTTCCACCTCTTCTTCAAGGGTGAGAAGTTGAGGTTGCAGAGTCGAATAGGCACACACGGGATTAAGCCAGGTGAGGTTCTAGTGCTGGTCCCGTTTGTTAAAAAGGTGGCTAATAATCAGAGCCAGAAATGTGATCAATCTAATCCATCGTTGAATACTTCTGCTTATGGCTCCATGTCCAAGTTTGCGAACTCCGCGTGGTCTGATATGATGCAGGACTTGTCGTATTTGTGTGATGGTTCGAGTGATGGAACCTCGAGTGTGTCTAATATAGGGAGTTTTAGTGTTGGAGATGGAAGTGGAGATTGCTCTCGTGAAGTGAAGCGGAAAAGGGGGTTTGGTTGTGATGAGATGATATTGGATATGTTGTGGGCTAGTAGAAGCAAGAGTGTTCTTGATGAACAGAATTCTAGAAGGTTTGTTGAGCTTTTGGAGTCGGTGAATTGTTTATCGGATCCATATTCTGGGGACTGCATGTTGTGGAGAAGAAAAGCTAGCTTACAAGGCTTTGGCTTGCGGCTGCCTAACGGCAATGGTAATTCATGCTTGTGTCCAGAGTGGTTGAAGAAAATCATGAAGGCGTTTGCTTTCTTAAACATTTTTTCTGCACTAGTTCAACTTCAGCAGGAGAGGACAACCTCAACTCTATTGGAGCAAGCACTGGAACAGCTTGCAACATTTGGAGTTAAACTTGATATGCAGGACATGGAGCATCTTTCGGTTATTTCTCCCAAGGTAGTTCTTTTTGTGAAGAAGAATGCAGAGGAGACAAGTTCTGGTAATGCACTTGTTATTATTGAATGCTCGGCAGAACAGAACCATGGAGACAGAAATAATCATGAATCTGCTTGCAAACAGATGGATGTATCAAAGATTGTCAATGAATTGAAGATGCAGGAAAGTTCTTTCAAAAGTAATTTGTGGGAGGCTGTTGAGAGGTTAATGCTTATCAAAAGAGATGAGATTTCTTCCTTGGAAGACTTTCTCATTTCTGTGAAGGATTGTGGTGCTGCTGCAAGAGGAAATAATGTGAATCAAGCAACGAAAAGCCACACAGCTTCAACTACTGATTCAGACAAAAAGCACTCTGCAGCTTCAACCTCTCGTTCAGACAAAAAGCGAGCGGGATGTCGTAGAACAGATCCATTGCTACCAGTTGAGATGGTTGAACATCTTAGAAAGAGAATTGGATCAGCAACGAAGATAGTGCATGTCGAAGACATTGGTGCTCGAAATGCCGTTCATGTGGAAGTTCCACAGGAACTATCAGAAAATTTGAGATCTGTACTTCATTCTATTGGAATCACTCAGTTATATAGCCATCAGGCAGAGTCGATACAAGCCTCACTAACTGGTAAGAATGTTGTTGTGGCTACAATGACATCCAGTGGGAAATCTCTTTGCTACAATCTACCGGTCATGGAAGCCCTGTCCCAGAGTTCTTCATCATGTGCTCTGTACTTATTTCCAACGAAGGCTTTAGCTCAAGATCAACTAAGAGCTTTCTCAGCTATGACCAAAGGATTCAGTGGTAGCTTAAATGTTGGTATATATGATGGTGACACTAATCAGCAGGAAAGGACACGACTGCGTCAAAATGCTAGACTGCTAATCACAAATCCAGATATGTTGCATTTATCAATCCTGCCACACCACCGAAAATTTGGCCGGATTTTGGCAAATCTTAGGTATGTAGTGATTGATGAGGCCCATGTTTATAAGGGAGCATTTGGTTGTCACACTGCTCTTATACTTAGGCGACTTCGCCGGCTCTGTTCACATGTGTATGGAAGTGATCCTTCTTTTGTGTTTTCTACTGCGACTTCGGCAAATCCTCGTGAGCATTGCATGGAACTTGCAAATTTACCAACCCTAGAGTTGATTAAGAATGATGGAAGCCCTGCTGCTAGAAAGCTTTTCATCCTTTGGAATTCTGAGGACAGCGCAATTGAGGATATATCATTCCTCTTTGCTGAAATGGTGCAGCATGGACTACGGTGCATTGCATTTTGCAAAACACGCAAGCTTTGTGAACTTGTTTTTTGCTATACGCGTGAAATTCTTCAGGAGACAGCACCCCATCTGGCAGAGTCTATATGTTCTTATCGTGCTGGTTACATTGCTGAGGATAGGAGAAGAATAGAGAGTGATTTATTTAATGGGAAACTTTCTGGTATTGCTGCAACAAATGCCCTTGAATTGGGTATTGATGTCGGACACATTGATGTAACTCTTCATTTAGGTTTTCCCGGAAGTATTGCTAGCTTGTGGCAACAAGCTGGCAGAGCCGGAAGGAGAGAGAGACCTTCTCTTGCTGTATATGTTGCATCCGAAGGGCCCCTTGACCAATATTTTATGAAACATCCAAATAAACTTTTTGGCAGCCCAATTGAGTGCTGTCATGTTGATGCTAAGAACCAACAGGTTCTTGAACAGCATTTGGCTTGTGCTGCTCTTGAACACCCACTGAGCTTGTTTTATGATGAGAAGTTTTTCGGTTCTGGTTTAAACAGCGCCATAATGTCTCTTAAAAGTAGAGGAAATCTGAGTTATGATCCATCATGTGATTCTTCTGCTAAAATCTGGAACTATATTGGGTATGAGAAAATGCCTTCACATTCAGTTAGTATTCGATCAATTGAAAGAGAGAGATACAAAGTTATGGACCATCAAGAGAAAGAAATCGAAGAGATTGAGGAAAGCAAGGCTTTCTTTCAGGTATATGAAGGTGCTGTTTATTTGAACCAAGGAAAGACATACCTGGTCACGAGCTTAGATTTATCTAGAAAGATTGCTTGGTGCCAAAAAGCTGATGTAAATTATTATACGAAACCCCGTGATCGCACAGATATTCATGTAGTTGGTGGTAATGTGGCTTATCCAGCTCCAGCTAAGCTTTCCAACCAGTTCTCCAGAACAACTGCCCGAGCAGATCCTTGCAGAGTAGCAACTACATGGTTTGGATTCTACCGTGTACGGAAAGGAAGCAACATGATATTAGATAAAGTTAACCTTGCACTTCCTAAATATACGTATGCGTCACAGGCAGTTTGGGTTCCGGTTCCACGATCAATAATTAAAGAAGTGGAGAGTAAAGAACTTGAATACCGTGCAGGGCTGCATGCTGCATCACATGCTGTTCTGCACGTTGTGCCTCTACGAATCATTTGTAACTTATCTGACTTGGCTCCAGAGTGTGCGGACCCTCATGACGGCAGATATTTTCCGGAGAGGATTCTATTATATGATCAGCATCCTGGAGGGACTGGTGTCTGTGTACAGGTTCATCCATTTTTTATGGAGCTCTTGGCTACTGCTCTGGAACTCCTAACATCTTGCCACTGCAGAGAATATAATGGTTGCCCTAATTGCATTCAGAGTTTTTGTTGCAAGTCATATAATGAGGTTTTACACAAGGATGCAGCAATTATGATCATTAAGGGTGTTCTGGATGCGAATAAATCTTCTGACTCTTCCGGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DUF1998 Helicase_C

Protein Analysis

1209

Amino Acids

134.21

Weight (kDa)

8.01

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 484 - 621 5.1e-07 Type III restriction enzyme, res subunit
DEAD PF00270 487 - 657 5.5e-31 DEAD/DEAH box helicase
Helicase_C PF00271 707 - 813 1.8e-12 Helicase conserved C-terminal domain
WHD_HRQ1 PF22982 849 - 927 3.3e-17 ATP-dependent helicase HRQ1, winged helix domain
MZB PF09369 1090 - 1172 1.4e-17 MrfA Zn-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1860
AccB1I GGYRCC 1 cut(s) 2940
AccB7I CCANNNNNTGG 1 cut(s) 605
AccBSI CCGCTC 1 cut(s) 1265
AccII CGCG 2 cut(s) 338, 2194
AccIII TCCGGA 2 cut(s) 3367, 3617
AciI CCGC 6 cut(s) 123, 336, 466, 658, 1265, 3341
AclWI GGATC 7 cut(s) 590, 603, 1277, 1339, 1931, 2690, 3068
AcoI YGGCCR 1 cut(s) 1807
AcsI RAATTY 6 cut(s) 547, 1414, 1802, 2002, 2071, 2199
AcuI CTGAAG 2 cut(s) 746, 2189
AcyI GRCGYC 1 cut(s) 78
AdeI CACNNNGTG 1 cut(s) 3292
AfaI GTAC 5 cut(s) 34, 1428, 1601, 3117, 3420
AfiI CCNNNNNNNGG 4 cut(s) 605, 1816, 2341, 3353
AflIII ACRYGT 2 cut(s) 1921, 2192
AgeI ACCGGT 1 cut(s) 1555
AhlI ACTAGT 1 cut(s) 751
AjnI CCWGG 3 cut(s) 208, 2901, 3397
AjuI GAANNNNNNNTTGG 2 cut(s) 2920, 2952
Alw21I GWGCWC 3 cut(s) 1372, 1597, 3447
Alw26I GTCTC 6 cut(s) 64, 902, 960, 2204, 2437, 2670
AlwI GGATC 7 cut(s) 590, 603, 1277, 1339, 1931, 2690, 3068
Ama87I CYCGRG 2 cut(s) 397, 3066
Aor13HI TCCGGA 2 cut(s) 3367, 3617
AoxI GGCC 3 cut(s) 1807, 1848, 2477
ApaI GGGCCC 1 cut(s) 2481
ApoI RAATTY 6 cut(s) 547, 1414, 1802, 2002, 2071, 2199
AsiGI ACCGGT 1 cut(s) 1555
Asp700I GAANNNNTTC 3 cut(s) 1122, 2202, 2633
AspLEI GCGC 2 cut(s) 2087, 2657
AspS9I GGNCC 7 cut(s) 28, 229, 1849, 2477, 2478, 2806, 3343
AsuC2I CCSGG 1 cut(s) 2391
AsuHPI GGTGA 4 cut(s) 173, 224, 592, 1712
AsuNHI GCTAGC 2 cut(s) 635, 2402
AvaI CYCGRG 2 cut(s) 397, 3066
AvaII GGWCC 4 cut(s) 28, 229, 2806, 3343
BaeGI GKGCMC 1 cut(s) 2481
BamHI GGATCC 1 cut(s) 595
BanI GGYRCC 1 cut(s) 2940
BanII GRGCYC 2 cut(s) 2481, 3447
BauI CACGAG 3 cut(s) 453, 1977, 2908
BbsI GAAGAC 2 cut(s) 1125, 1365
Bbv12I GWGCWC 3 cut(s) 1372, 1597, 3447
BbvCI CCTCAGC 1 cut(s) 2265
BceAI ACGGC 4 cut(s) 65, 685, 1364, 3370
BcgI CGANNNNNNTGC 2 cut(s) 1330, 1364
BciT130I CCWGG 3 cut(s) 210, 2903, 3399
BclI TGATCA 3 cut(s) 274, 3388, 3573
BcnI CCSGG 1 cut(s) 2391
BcoDI GTCTC 6 cut(s) 64, 902, 960, 2204, 2437, 2670
BcuI ACTAGT 1 cut(s) 751
BfmI CTRYAG 2 cut(s) 1230, 3484
BfoI RGCGCY 1 cut(s) 2658
BglII AGATCT 1 cut(s) 1421
BlpI GCTNAGC 1 cut(s) 3033
Bme1390I CCNGG 4 cut(s) 210, 2391, 2903, 3399
Bme18I GGWCC 4 cut(s) 28, 229, 2806, 3343
BmeT110I CYCGRG 2 cut(s) 397, 3066
BmgT120I GGNCC 7 cut(s) 28, 229, 1849, 2477, 2478, 2806, 3343
BmrFI CCNGG 4 cut(s) 210, 2391, 2903, 3399
BmsI GCATC 9 cut(s) 342, 862, 1023, 2477, 2539, 3278, 3403, 3550, 3583
BmtI GCTAGC 2 cut(s) 639, 2406
BoxI GACNNNNGTC 2 cut(s) 56, 3410
BpiI GAAGAC 2 cut(s) 1125, 1365
BplI GAGNNNNNCTC 2 cut(s) 2434, 2466
BpmI CTGGAG 4 cut(s) 3012, 3037, 3315, 3420
Bpu10I CCTNAGC 1 cut(s) 2265
Bpu1102I GCTNAGC 1 cut(s) 3033
BpuEI CTTGAG 2 cut(s) 77, 1611
BpuMI CCSGG 1 cut(s) 2391
BsaAI YACGTR 1 cut(s) 3174
BsaBI GATNNNNATC 2 cut(s) 20, 3393
BsaHI GRCGYC 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 2437
BsaJI CCNNGG 4 cut(s) 873, 961, 1113, 2887
BsaWI WCCGGW 4 cut(s) 1555, 3199, 3367, 3617
BsaXI ACNNNNNCTCC 4 cut(s) 409, 439, 3035, 3065
Bsc4I CCNNNNNNNGG 4 cut(s) 605, 1816, 2341, 3353
Bse118I RCCGGY 3 cut(s) 41, 1555, 1908
Bse1I ACTGG 7 cut(s) 109, 807, 1295, 1498, 1527, 3046, 3412
Bse3DI GCAATG 5 cut(s) 679, 1286, 1984, 2142, 2259
Bse8I GATNNNNATC 2 cut(s) 20, 3393
BseAI TCCGGA 2 cut(s) 3367, 3617
BseBI CCWGG 3 cut(s) 210, 2903, 3399
BseDI CCNNGG 4 cut(s) 873, 961, 1113, 2887
BseJI GATNNNNATC 2 cut(s) 20, 3393
BseLI CCNNNNNNNGG 4 cut(s) 605, 1816, 2341, 3353
BseMI GCAATG 5 cut(s) 679, 1286, 1984, 2142, 2259
BseMII CTCAG 7 cut(s) 75, 1463, 1662, 2067, 2256, 2600, 2678
BseNI ACTGG 7 cut(s) 109, 807, 1295, 1498, 1527, 3046, 3412
BseRI GAGGAG 1 cut(s) 920
BseSI GKGCMC 1 cut(s) 2481
BsgI GTGCAG 4 cut(s) 732, 2144, 3269, 3273
Bsh1236I CGCG 2 cut(s) 338, 2194
BshFI GGCC 3 cut(s) 1809, 1850, 2479
BshNI GGYRCC 1 cut(s) 2940
BshTI ACCGGT 1 cut(s) 1555
BsiHKAI GWGCWC 3 cut(s) 1372, 1597, 3447
BsiHKCI CYCGRG 2 cut(s) 397, 3066
BsiSI CCGG 9 cut(s) 42, 1556, 1810, 1909, 2391, 2430, 3200, 3368, 3618
BslFI GGGAC 4 cut(s) 215, 623, 1558, 3418
BslI CCNNNNNNNGG 4 cut(s) 605, 1816, 2341, 3353
BsmAI GTCTC 6 cut(s) 64, 902, 960, 2204, 2437, 2670
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 4 cut(s) 215, 623, 1558, 3418
BsmI GAATGC 3 cut(s) 904, 947, 3513
BsnI GGCC 3 cut(s) 1809, 1850, 2479
Bso31I GGTCTC 1 cut(s) 2437
BsoBI CYCGRG 2 cut(s) 397, 3066
Bsp120I GGGCCC 1 cut(s) 2477
Bsp1286I GDGCHC 4 cut(s) 1372, 1597, 2481, 3447
Bsp13I TCCGGA 2 cut(s) 3367, 3617
Bsp1407I TGTACA 1 cut(s) 3418
Bsp1720I GCTNAGC 1 cut(s) 3033
Bsp19I CCATGG 1 cut(s) 961
BspACI CCGC 6 cut(s) 123, 336, 466, 658, 1265, 3341
BspANI GGCC 3 cut(s) 1809, 1850, 2479
BspCNI CTCAG 7 cut(s) 74, 1462, 1661, 2068, 2257, 2601, 2679
BspEI TCCGGA 2 cut(s) 3367, 3617
BspFNI CGCG 2 cut(s) 338, 2194
BspHI TCATGA 3 cut(s) 714, 979, 3349
BspMAI CTGCAG 2 cut(s) 1234, 3488
BspOI GCTAGC 2 cut(s) 639, 2406
BspPI GGATC 7 cut(s) 590, 603, 1277, 1339, 1931, 2690, 3068
BspT107I GGYRCC 1 cut(s) 2940
BspTNI GGTCTC 1 cut(s) 2437
BsrBI CCGCTC 1 cut(s) 1265
BsrDI GCAATG 5 cut(s) 679, 1286, 1984, 2142, 2259
BsrFI RCCGGY 3 cut(s) 41, 1555, 1908
BsrGI TGTACA 1 cut(s) 3418
BsrI ACTGG 7 cut(s) 109, 807, 1295, 1498, 1527, 3046, 3412
BssAI RCCGGY 3 cut(s) 41, 1555, 1908
BssECI CCNNGG 4 cut(s) 873, 961, 1113, 2887
BssNI GRCGYC 1 cut(s) 78
BssSI CACGAG 3 cut(s) 453, 1977, 2908
BssT1I CCWWGG 4 cut(s) 873, 961, 1113, 2887
Bst2BI CACGAG 3 cut(s) 453, 1977, 2908
Bst2UI CCWGG 3 cut(s) 210, 2903, 3399
Bst4CI ACNGT 4 cut(s) 58, 2139, 3113, 3251
Bst6I CTCTTC 4 cut(s) 155, 2379, 2823, 3619
BstACI GRCGYC 1 cut(s) 78
BstAPI GCANNNNNTGC 3 cut(s) 2330, 3275, 3292
BstAUI TGTACA 1 cut(s) 3418
BstBAI YACGTR 1 cut(s) 3174
BstDSI CCRYGG 1 cut(s) 961
BstFNI CGCG 2 cut(s) 338, 2194
BstH2I RGCGCY 1 cut(s) 2658
BstHHI GCGC 2 cut(s) 2087, 2657
BstMAI GTCTC 6 cut(s) 64, 902, 960, 2204, 2437, 2670
BstNI CCWGG 3 cut(s) 210, 2903, 3399
BstNSI RCATGY 5 cut(s) 619, 1355, 1925, 3266, 3278
BstPAI GACNNNNGTC 2 cut(s) 56, 3410
BstSCI CCNGG 4 cut(s) 208, 2389, 2901, 3397
BstSFI CTRYAG 2 cut(s) 1230, 3484
BstSLI GKGCMC 1 cut(s) 2481
BstSNI TACGTA 1 cut(s) 3174
BstUI CGCG 2 cut(s) 338, 2194
BstV2I GAAGAC 2 cut(s) 1125, 1365
BstX2I RGATCY 5 cut(s) 595, 1282, 1421, 3073, 3620
BstYI RGATCY 5 cut(s) 595, 1282, 1421, 3073, 3620
BsuRI GGCC 3 cut(s) 1809, 1850, 2479
BtgI CCRYGG 1 cut(s) 961
BtsI GCAGTG 2 cut(s) 1881, 3481
BtsIMutI CAGTG 6 cut(s) 800, 1534, 1675, 1881, 2606, 3481
CciI TCATGA 3 cut(s) 714, 979, 3349
CfoI GCGC 2 cut(s) 2087, 2657
Cfr10I RCCGGY 3 cut(s) 41, 1555, 1908
Cfr13I GGNCC 7 cut(s) 28, 229, 1849, 2477, 2478, 2806, 3343
CseI GACGC 3 cut(s) 86, 1721, 3168
CsiI ACCWGGT 1 cut(s) 2901
Csp6I GTAC 5 cut(s) 33, 1427, 1600, 3116, 3419
CspAI ACCGGT 1 cut(s) 1555
CspCI CAANNNNNGTGG 2 cut(s) 2595, 2630
CviQI GTAC 5 cut(s) 33, 1427, 1600, 3116, 3419
DraI TTTAAA 1 cut(s) 2649
DraIII CACNNNGTG 1 cut(s) 3292
EaeI YGGCCR 1 cut(s) 1807
Eam1104I CTCTTC 4 cut(s) 155, 2379, 2823, 3619
EarI CTCTTC 4 cut(s) 155, 2379, 2823, 3619
Ecl136II GAGCTC 1 cut(s) 3445
Eco105I TACGTA 1 cut(s) 3174
Eco130I CCWWGG 4 cut(s) 873, 961, 1113, 2887
Eco24I GRGCYC 2 cut(s) 2481, 3447
Eco31I GGTCTC 1 cut(s) 2437
Eco47I GGWCC 4 cut(s) 28, 229, 2806, 3343
Eco53kI GAGCTC 1 cut(s) 3445
Eco57I CTGAAG 2 cut(s) 746, 2189
Eco88I CYCGRG 2 cut(s) 397, 3066
EcoICRI GAGCTC 1 cut(s) 3445
EcoO109I RGGNCCY 2 cut(s) 2477, 2478
EcoRI GAATTC 2 cut(s) 547, 2071
EcoRII CCWGG 3 cut(s) 208, 2901, 3397
EcoT14I CCWWGG 4 cut(s) 873, 961, 1113, 2887
EcoT38I GRGCYC 2 cut(s) 2481, 3447
ErhI CCWWGG 4 cut(s) 873, 961, 1113, 2887
Esp3I CGTCTC 1 cut(s) 64
FaqI GGGAC 4 cut(s) 215, 623, 1558, 3418
FauI CCCGC 2 cut(s) 130, 1258
FbaI TGATCA 3 cut(s) 274, 3388, 3573
FriOI GRGCYC 2 cut(s) 2481, 3447
GlaI GCGC 2 cut(s) 2086, 2656
GsuI CTGGAG 4 cut(s) 3012, 3037, 3315, 3420
HaeII RGCGCY 1 cut(s) 2658
HaeIII GGCC 3 cut(s) 1809, 1850, 2479
HapII CCGG 9 cut(s) 42, 1556, 1810, 1909, 2391, 2430, 3200, 3368, 3618
HgaI GACGC 3 cut(s) 86, 1721, 3168
HhaI GCGC 2 cut(s) 2087, 2657
Hin1I GRCGYC 1 cut(s) 78
Hin6I GCGC 2 cut(s) 2085, 2655
HinP1I GCGC 2 cut(s) 2085, 2655
HincII GTYRAC 2 cut(s) 73, 3151
HindII GTYRAC 2 cut(s) 73, 3151
HindIII AAGCTT 3 cut(s) 2054, 2165, 3035
HpaI GTTAAC 1 cut(s) 3151
HpaII CCGG 9 cut(s) 42, 1556, 1810, 1909, 2391, 2430, 3200, 3368, 3618
HphI GGTGA 4 cut(s) 173, 224, 592, 1712
Hpy166II GTNNAC 6 cut(s) 73, 1919, 2174, 3116, 3151, 3419
Hpy8I GTNNAC 6 cut(s) 73, 1919, 2174, 3116, 3151, 3419
Hpy99I CGWCG 1 cut(s) 80
HpyCH4III ACNGT 4 cut(s) 58, 2139, 3113, 3251
HpyCH4IV ACGT 2 cut(s) 3173, 3288
HpySE526I ACGT 2 cut(s) 3173, 3288
Hsp92I GRCGYC 1 cut(s) 78
HspAI GCGC 2 cut(s) 2085, 2655
Kpn2I TCCGGA 2 cut(s) 3367, 3617
KroI GCCGGC 1 cut(s) 1908
KroNI GCCGGC 1 cut(s) 1910
Ksp22I TGATCA 3 cut(s) 274, 3388, 3573
KspAI GTTAAC 1 cut(s) 3151
LmnI GCTCC 7 cut(s) 317, 793, 850, 1865, 3031, 3334, 3442
LweI GCATC 9 cut(s) 342, 862, 1023, 2477, 2539, 3278, 3403, 3550, 3583
MabI ACCWGGT 1 cut(s) 2901
MaeII ACGT 2 cut(s) 3173, 3288
MaeIII GTNAC 7 cut(s) 1700, 1877, 2255, 2368, 2905, 3180, 3311
MbiI CCGCTC 1 cut(s) 1265
MfeI CAATTG 3 cut(s) 2088, 2529, 2778
MflI RGATCY 5 cut(s) 595, 1282, 1421, 3073, 3620
MhlI GDGCHC 4 cut(s) 1372, 1597, 2481, 3447
MluI ACGCGT 1 cut(s) 2192
MlyI GAGTC 6 cut(s) 19, 190, 584, 1481, 2240, 3605
MmeI TCCRAC 4 cut(s) 409, 1634, 2335, 3066
MroI TCCGGA 2 cut(s) 3367, 3617
MroNI GCCGGC 1 cut(s) 1908
MroXI GAANNNNTTC 3 cut(s) 1122, 2202, 2633
MslI CAYNNNNRTG 6 cut(s) 688, 1133, 1926, 2364, 2547, 3558
MspI CCGG 9 cut(s) 42, 1556, 1810, 1909, 2391, 2430, 3200, 3368, 3618
MspR9I CCNGG 4 cut(s) 210, 2391, 2903, 3399
MssI GTTTAAAC 1 cut(s) 2649
MunI CAATTG 3 cut(s) 2088, 2529, 2778
Mva1269I GAATGC 3 cut(s) 904, 947, 3513
MvaI CCWGG 3 cut(s) 210, 2903, 3399
MvnI CGCG 2 cut(s) 338, 2194
NaeI GCCGGC 1 cut(s) 1910
NciI CCSGG 1 cut(s) 2391
NcoI CCATGG 1 cut(s) 961
NgoMIV GCCGGC 1 cut(s) 1908
NheI GCTAGC 2 cut(s) 635, 2402
NmeAIII GCCGAG 1 cut(s) 926
NmuCI GTSAC 4 cut(s) 1700, 1877, 2905, 3180
NspI RCATGY 5 cut(s) 619, 1355, 1925, 3266, 3278
PaeI GCATGC 1 cut(s) 3266
PaeR7I CTCGAG 1 cut(s) 397
PagI TCATGA 3 cut(s) 714, 979, 3349
PciI ACATGT 1 cut(s) 1921
PctI GAATGC 3 cut(s) 904, 947, 3513
PdiI GCCGGC 1 cut(s) 1910
PdmI GAANNNNTTC 3 cut(s) 1122, 2202, 2633
PflFI GACNNNGTC 1 cut(s) 1731
PflMI CCANNNNNTGG 1 cut(s) 605
PfoI TCCNGGA 2 cut(s) 2389, 3397
PinAI ACCGGT 1 cut(s) 1555
PleI GAGTC 6 cut(s) 18, 189, 583, 1480, 2239, 3605
PmeI GTTTAAAC 1 cut(s) 2649
PpsI GAGTC 6 cut(s) 18, 189, 583, 1480, 2239, 3605
Ppu21I YACGTR 1 cut(s) 3174
PscI ACATGT 1 cut(s) 1921
PshAI GACNNNNGTC 2 cut(s) 56, 3410
PsiI TTATAA 1 cut(s) 1860
Psp124BI GAGCTC 1 cut(s) 3447
Psp6I CCWGG 3 cut(s) 208, 2901, 3397
PspGI CCWGG 3 cut(s) 208, 2901, 3397
PspOMI GGGCCC 1 cut(s) 2477
PspPI GGNCC 7 cut(s) 28, 229, 1849, 2477, 2478, 2806, 3343
PspXI VCTCGAGB 1 cut(s) 397
PstI CTGCAG 2 cut(s) 1234, 3488
PsuI RGATCY 5 cut(s) 595, 1282, 1421, 3073, 3620
PsyI GACNNNGTC 1 cut(s) 1731
RsaI GTAC 5 cut(s) 34, 1428, 1601, 3117, 3420
RsaNI GTAC 5 cut(s) 33, 1427, 1600, 3116, 3419
RseI CAYNNNNRTG 6 cut(s) 688, 1133, 1926, 2364, 2547, 3558
SacI GAGCTC 1 cut(s) 3447
Sau96I GGNCC 7 cut(s) 28, 229, 1849, 2477, 2478, 2806, 3343
SchI GAGTC 6 cut(s) 19, 190, 584, 1481, 2240, 3605
ScrFI CCNGG 4 cut(s) 210, 2391, 2903, 3399
SduI GDGCHC 4 cut(s) 1372, 1597, 2481, 3447
SexAI ACCWGGT 1 cut(s) 2901
SfaNI GCATC 9 cut(s) 342, 862, 1023, 2477, 2539, 3278, 3403, 3550, 3583
SfcI CTRYAG 2 cut(s) 1230, 3484
Sfr274I CTCGAG 1 cut(s) 397
SinI GGWCC 4 cut(s) 28, 229, 2806, 3343
SlaI CTCGAG 1 cut(s) 397
SmiMI CAYNNNNRTG 6 cut(s) 688, 1133, 1926, 2364, 2547, 3558
SmlI CTYRAG 3 cut(s) 92, 397, 1626
SmoI CTYRAG 3 cut(s) 92, 397, 1626
SnaBI TACGTA 1 cut(s) 3174
SpeI ACTAGT 1 cut(s) 751
SphI GCATGC 1 cut(s) 3266
SsiI CCGC 6 cut(s) 123, 336, 466, 658, 1265, 3341
SspI AATATT 1 cut(s) 2492
SstI GAGCTC 1 cut(s) 3447
StyD4I CCNGG 4 cut(s) 208, 2389, 2901, 3397
StyI CCWWGG 4 cut(s) 873, 961, 1113, 2887
TaaI ACNGT 4 cut(s) 58, 2139, 3113, 3251
TaiI ACGT 2 cut(s) 3176, 3291
TaqI TCGA 9 cut(s) 24, 184, 383, 398, 1356, 1372, 1475, 2773, 2826
TatI WGTACW 3 cut(s) 1426, 1599, 3418
TauI GCSGC 1 cut(s) 661
TscAI CASTG 6 cut(s) 807, 1534, 1675, 1888, 2613, 3488
TseFI GTSAC 4 cut(s) 1700, 1877, 2905, 3180
Tsp45I GTSAC 4 cut(s) 1700, 1877, 2905, 3180
TspGWI ACGGA 3 cut(s) 20, 58, 3133
TspRI CASTG 6 cut(s) 807, 1534, 1675, 1888, 2613, 3488
Tth111I GACNNNGTC 1 cut(s) 1731
Van91I CCANNNNNTGG 1 cut(s) 605
VpaK11BI GGWCC 4 cut(s) 28, 229, 2806, 3343
XapI RAATTY 6 cut(s) 547, 1414, 1802, 2002, 2071, 2199
XbaI TCTAGA 2 cut(s) 551, 2924
XceI RCATGY 5 cut(s) 619, 1355, 1925, 3266, 3278
XcmI CCANNNNNNNNNTGG 1 cut(s) 1802
XhoI CTCGAG 1 cut(s) 397
XmnI GAANNNNTTC 3 cut(s) 1122, 2202, 2633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.