RchiOBHm_Chr7g0183711

ATP-dependent helicase hrq1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0006845
Physical Location & Seq
Reverse (-)
4487633 .. 4494888
7256 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16391

Sequence Viewer

Length: 2790 bp
ATGCAGGACATGGAGCATCTTTCGGTTATTTCTCCCAAGGTAGTTCTTTTTGTGAAGAAGAATGCAGAGGAGACAAGTTCTGGTAATGCACTAGTTATTATTGAATGCTCGGCAGAACAGAACCATGGAGACAGAAATAATCATGAATCTGCTTCGAAACAGATGGATGTATCAAAGATTGTCAATGAATTGAAGATGCAGGAAAGTTCTTTCAAAAGTAATTTGTGGGAGGCTGTTGAGAGGTTAATGTTTATCAAAAGAAATGAGATTTCCTCCTTGGAAGACTTTCTCATTTCTGTGAAGGATTGCGGTGCTGCTGCAAGAGGAAATAAAGTTAATCAAGCAACGAAAAGCCACACAGCTTCAACTACTGATTCAGACAAAAAGCACTCTGCAGCTTCAACCTCTCGTTCACACAAAAAGCGAGCACTATGTCATAGAACAGGTCCATTGCTACCAGTTGAGATGGTTGAACATCTTAGAAAGAGAATTGGATCAGCAACGAAGATAGTGCATGTCGAAGACATTGGTGCTCGAAATGCCGTTCATGTGGAAGTTCCACAGGAACTATCAGAAAATCTGAGATCTGTACTTCATTCTATTGGAATCACTCAGTTATATAGCCATCAGGCAGAGTCGATACAAGCCTCACTAACTGGCAAGAATGTTGTTGTGGCTACAATGACATCCAGTGGGAAATCTCTTTGCTACAATCTACCGGTCATTGAAGCCCTGTCCCAGAGTTCCTCATCATGTGCTCTATACTTATTTCCAACGAAGGCTTTAGCTCAAGATCAACTAAGAGCTTTCTTAGCTATGACCAAAGGATTCAGTGGTAGCTTAAATGTTGGTATATATGATGGTGACACTAATCAGCAGGAAAGGACACGACTGCGTCAAAATGCTAGACTGCTAATCACAAATCCAGATATGTTGCATGTATCAATCCTGCCACACCACCGAAAATTTGGCCGGATTTTGGCAAATCTTAGGTATGTAGTGATTGATGAGGCCCATGTTTATAACGGAGCATTTGGTTGTCACACTGCTCTTATACTTAGGCGACTTCGCCGGCTCTGTTCACATGTGTATGGAAGTGATCCTTCTTTTGTGTTTTCTACTGCGACTTCAGCAAATCCTCGTGAGCATTGCATGGAACTTGCGAATTTACCAACTCTAGAGTTGATTCAGAATGATGGAAGCCCTGCTGCTAGAAAGCTTTTCATCCTTTGGAATTCTGAGGACAGCGCAATTGAGGATATATCATTCCTGTTTGCTGAAATGGTCCAGCATGGACTACGGTGCATTGCATTTTGCAAAACACGCAAGCTTTGTGAACTTGTTTTTTGCCATACGCGTGAAATTCTTCAGGAGACAGCACCCCATCTGGCAGAGTCTATATGTTCTTATCGTGCTGGTTACATTGCTGAGGATAGGAGAAGAATAGAGAGTGATTTATTTAACGGGAAACTTTCTGGTATTGCTGCAACAAATGCCCTTGAATTGGGTATTGATGTCGGACACATTGATGTAACTCTTCATTTGGGTTTTCCCGGAAGTATTGCTAGCTTGTGGCAACAAGCTGGCAGAGCCGGAAGGAGAGAGAGACCTTCTCTTGCTGTATATGTTGCATTCGAAGGGCCCCTTGACCAATATTTTATGAAACATCCAAATAAACTTTTTGGAAGCCCAATTGAGTGCTGTCATGTTGATGCTAAGAACCAACAGGTTCTTGAACAGCATTTGGCTTGTGCTGCTCTTGAACACCCACTGAGCTTGTTTTATGATGGGAAGTTTTTCGGTTCTGGCTTAAACAGCGCCATAATGTCTCTTAAAAGTAGAGGATTTCTGAGTTATGATCCATCATGTGATTCTGCTGCTAAAATCTGGAACTATATTGGGTATCAGAAAATGCCTTCACATTCAGTTAGTATTCGATCAATTGAAAGAGAGAGATACAAAGTTATGGACGATCAAGAGAAAGAAATCGAAGAGATTGAGGAAAGCAAGGCTTTCTTTCAGGTATATGAAGGTGCTGTTTATTTGAACCAAGGAAAGACATACCTGGTCACGAGCTTAGATTTATCTAGCAAGATTGCTTGGTGCCAAAAAGCTGATGTAAATTATTATACGAAACCCCGTGATCGCACAGATATTCATGTAGTTGGTGGTAATGTGGCTTATCCAGCTCCAGCTAAGCTTTCCAACCAGTTCTCCAGAACAACTGCCCGAGCAGATCCTTGCAGAGTAACAACTACATGGTTTGGATTCTATCGTGTACACAAAGGAAGCAACATGATATTTGATAAAGTTAACCTTGCACTTCCTAAATATACGTATACGTCACAGGCAGTTTGGGTTCCGGTTCCACGATCAATAATTAAAGCAGTGGAGAGTAAACAACTTGAATACCGTGCAGGGCTGCATGCTGCATCACATGCTGTTCTGCACGTTGTGCCTCTACGAATCATTTGTAACTTATCTGACTTGGCTCCAGAGTGTGCGGACCCTCATGACGGCAGCTATTTTCCGGAGAGGATTCTATTATATGATCAGCATCCTGGAGGGACTGGTGTCTGTGTACAGGTTCATCCATTTTTTATGGAGCTCTTGGCTACTGCTCTGGAACTCCTGACATCTTGCCACTGCAGAGAAGATGATGGTTGCCCTAATTGCATTCAGAGTTTTTGTTGCAAGTCATATAATGAGGTTTTACACAAGGATGCAGCAATTATGATCATTAAGGGTATTCTGGATGCGAATATATCTTCTGACTCTTCTGGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DUF1998 Helicase_C

Protein Analysis

929

Amino Acids

103.38

Weight (kDa)

7.39

Isoelectric Point (pI)

45.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 205 - 343 2.3e-07 Type III restriction enzyme, res subunit
DEAD PF00270 208 - 378 6.1e-31 DEAD/DEAH box helicase
Helicase_C PF00271 428 - 534 1.1e-12 Helicase conserved C-terminal domain
WHD_HRQ1 PF22982 570 - 648 4.2e-16 ATP-dependent helicase HRQ1, winged helix domain
MZB PF09369 811 - 893 5.6e-18 MrfA Zn-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1023
AccB1I GGYRCC 1 cut(s) 2103
AccI GTMKAC 1 cut(s) 2339
AccII CGCG 1 cut(s) 1357
AccIII TCCGGA 1 cut(s) 2530
AciI CCGC 2 cut(s) 309, 2504
AclWI GGATC 4 cut(s) 502, 1094, 1853, 2231
AcoI YGGCCR 1 cut(s) 970
AcsI RAATTY 4 cut(s) 965, 1165, 1234, 1362
AcuI CTGAAG 2 cut(s) 1113, 1352
AdeI CACNNNGTG 1 cut(s) 2455
AfaI GTAC 3 cut(s) 591, 2280, 2583
AfiI CCNNNNNNNGG 3 cut(s) 979, 1504, 2516
AflIII ACRYGT 2 cut(s) 1084, 1355
AgeI ACCGGT 1 cut(s) 718
AhlI ACTAGT 1 cut(s) 91
AjnI CCWGG 2 cut(s) 2064, 2560
Alw21I GWGCWC 4 cut(s) 430, 535, 760, 2610
Alw26I GTCTC 5 cut(s) 65, 123, 1367, 1600, 1833
AlwI GGATC 4 cut(s) 502, 1094, 1853, 2231
Ama87I CYCGRG 1 cut(s) 2229
Aor13HI TCCGGA 1 cut(s) 2530
AoxI GGCC 3 cut(s) 970, 1011, 1640
ApaI GGGCCC 1 cut(s) 1644
ApoI RAATTY 4 cut(s) 965, 1165, 1234, 1362
AsiGI ACCGGT 1 cut(s) 718
Asp700I GAANNNNTTC 3 cut(s) 285, 1365, 1796
AspLEI GCGC 2 cut(s) 1250, 1820
AspS9I GGNCC 6 cut(s) 446, 1012, 1285, 1640, 1641, 2506
AsuC2I CCSGG 1 cut(s) 1554
AsuHPI GGTGA 1 cut(s) 875
AsuII TTCGAA 2 cut(s) 155, 1635
AsuNHI GCTAGC 1 cut(s) 1565
AvaI CYCGRG 1 cut(s) 2229
AvaII GGWCC 3 cut(s) 446, 1285, 2506
BaeGI GKGCMC 1 cut(s) 1644
BanI GGYRCC 1 cut(s) 2103
BanII GRGCYC 2 cut(s) 1644, 2610
BauI CACGAG 2 cut(s) 1140, 2071
BbsI GAAGAC 2 cut(s) 288, 528
Bbv12I GWGCWC 4 cut(s) 430, 535, 760, 2610
BbvCI CCTCAGC 1 cut(s) 1428
BccI CCATC 9 cut(s) 157, 460, 633, 854, 1190, 1392, 1781, 1870, 2654
BceAI ACGGC 2 cut(s) 527, 2533
BcgI CGANNNNNNTGC 2 cut(s) 493, 527
BciT130I CCWGG 2 cut(s) 2066, 2562
BclI TGATCA 2 cut(s) 2551, 2736
BcnI CCSGG 1 cut(s) 1554
BcoDI GTCTC 5 cut(s) 65, 123, 1367, 1600, 1833
BcuI ACTAGT 1 cut(s) 91
BfaI CTAG 6 cut(s) 92, 906, 1178, 1212, 1566, 2088
BfmI CTRYAG 2 cut(s) 393, 2647
BfoI RGCGCY 1 cut(s) 1821
BglII AGATCT 1 cut(s) 584
BlpI GCTNAGC 1 cut(s) 2196
Bme1390I CCNGG 3 cut(s) 1554, 2066, 2562
Bme18I GGWCC 3 cut(s) 446, 1285, 2506
BmeT110I CYCGRG 1 cut(s) 2229
BmgT120I GGNCC 6 cut(s) 446, 1012, 1285, 1640, 1641, 2506
BmiI GGNNCC 7 cut(s) 1642, 1643, 2105, 2361, 2367, 2493, 2508
BmrFI CCNGG 3 cut(s) 1554, 2066, 2562
BmsI GCATC 7 cut(s) 25, 186, 1702, 2441, 2566, 2713, 2746
BmtI GCTAGC 1 cut(s) 1569
BoxI GACNNNNGTC 1 cut(s) 2573
BpiI GAAGAC 2 cut(s) 288, 528
BplI GAGNNNNNCTC 4 cut(s) 257, 289, 1597, 1629
BpmI CTGGAG 4 cut(s) 2175, 2200, 2478, 2583
Bpu10I CCTNAGC 1 cut(s) 1428
Bpu1102I GCTNAGC 1 cut(s) 2196
Bpu14I TTCGAA 2 cut(s) 155, 1635
BpuEI CTTGAG 1 cut(s) 774
BpuMI CCSGG 1 cut(s) 1554
BsaAI YACGTR 1 cut(s) 2337
BsaBI GATNNNNATC 1 cut(s) 2556
BsaI GGTCTC 1 cut(s) 1600
BsaJI CCNNGG 4 cut(s) 36, 124, 276, 2050
BsaWI WCCGGW 3 cut(s) 718, 2362, 2530
BsaXI ACNNNNNCTCC 2 cut(s) 2198, 2228
Bsc4I CCNNNNNNNGG 3 cut(s) 979, 1504, 2516
Bse118I RCCGGY 2 cut(s) 718, 1071
Bse1I ACTGG 5 cut(s) 458, 661, 690, 2209, 2575
Bse3DI GCAATG 4 cut(s) 449, 1147, 1305, 1422
Bse8I GATNNNNATC 1 cut(s) 2556
BseAI TCCGGA 1 cut(s) 2530
BseBI CCWGG 2 cut(s) 2066, 2562
BseDI CCNNGG 4 cut(s) 36, 124, 276, 2050
BseGI GGATG 8 cut(s) 172, 686, 1224, 1666, 2557, 2590, 2728, 2761
BseJI GATNNNNATC 1 cut(s) 2556
BseLI CCNNNNNNNGG 3 cut(s) 979, 1504, 2516
BseMI GCAATG 4 cut(s) 449, 1147, 1305, 1422
BseMII CTCAG 6 cut(s) 572, 626, 1230, 1419, 1763, 1841
BseNI ACTGG 5 cut(s) 458, 661, 690, 2209, 2575
BseRI GAGGAG 1 cut(s) 83
BseSI GKGCMC 1 cut(s) 1644
BsgI GTGCAG 2 cut(s) 2432, 2436
Bsh1236I CGCG 1 cut(s) 1357
BshFI GGCC 3 cut(s) 972, 1013, 1642
BshNI GGYRCC 1 cut(s) 2103
BshTI ACCGGT 1 cut(s) 718
BsiHKAI GWGCWC 4 cut(s) 430, 535, 760, 2610
BsiHKCI CYCGRG 1 cut(s) 2229
BsiSI CCGG 7 cut(s) 719, 973, 1072, 1554, 1593, 2363, 2531
BslFI GGGAC 2 cut(s) 721, 2581
BslI CCNNNNNNNGG 3 cut(s) 979, 1504, 2516
BsmAI GTCTC 5 cut(s) 65, 123, 1367, 1600, 1833
BsmFI GGGAC 2 cut(s) 721, 2581
BsmI GAATGC 4 cut(s) 67, 110, 1631, 2676
BsnI GGCC 3 cut(s) 972, 1013, 1642
Bso31I GGTCTC 1 cut(s) 1600
BsoBI CYCGRG 1 cut(s) 2229
Bsp119I TTCGAA 2 cut(s) 155, 1635
Bsp120I GGGCCC 1 cut(s) 1640
Bsp1286I GDGCHC 5 cut(s) 430, 535, 760, 1644, 2610
Bsp13I TCCGGA 1 cut(s) 2530
Bsp1407I TGTACA 2 cut(s) 2278, 2581
Bsp1720I GCTNAGC 1 cut(s) 2196
Bsp19I CCATGG 1 cut(s) 124
BspACI CCGC 2 cut(s) 309, 2504
BspANI GGCC 3 cut(s) 972, 1013, 1642
BspCNI CTCAG 6 cut(s) 573, 625, 1231, 1420, 1764, 1842
BspEI TCCGGA 1 cut(s) 2530
BspFNI CGCG 1 cut(s) 1357
BspHI TCATGA 2 cut(s) 142, 2512
BspLI GGNNCC 7 cut(s) 1642, 1643, 2105, 2361, 2367, 2493, 2508
BspMAI CTGCAG 2 cut(s) 397, 2651
BspOI GCTAGC 1 cut(s) 1569
BspPI GGATC 4 cut(s) 502, 1094, 1853, 2231
BspT104I TTCGAA 2 cut(s) 155, 1635
BspT107I GGYRCC 1 cut(s) 2103
BspTNI GGTCTC 1 cut(s) 1600
BsrDI GCAATG 4 cut(s) 449, 1147, 1305, 1422
BsrFI RCCGGY 2 cut(s) 718, 1071
BsrGI TGTACA 2 cut(s) 2278, 2581
BsrI ACTGG 5 cut(s) 458, 661, 690, 2209, 2575
BssAI RCCGGY 2 cut(s) 718, 1071
BssECI CCNNGG 4 cut(s) 36, 124, 276, 2050
BssNAI GTATAC 1 cut(s) 2340
BssSI CACGAG 2 cut(s) 1140, 2071
BssT1I CCWWGG 4 cut(s) 36, 124, 276, 2050
Bst1107I GTATAC 1 cut(s) 2340
Bst2BI CACGAG 2 cut(s) 1140, 2071
Bst2UI CCWGG 2 cut(s) 2066, 2562
Bst4CI ACNGT 2 cut(s) 1302, 2414
Bst6I CTCTTC 3 cut(s) 1542, 1986, 2782
BstAPI GCANNNNNTGC 3 cut(s) 1493, 2438, 2455
BstAUI TGTACA 2 cut(s) 2278, 2581
BstBAI YACGTR 1 cut(s) 2337
BstBI TTCGAA 2 cut(s) 155, 1635
BstC8I GCNNGC 6 cut(s) 426, 1073, 1328, 1567, 1585, 2427
BstDSI CCRYGG 1 cut(s) 124
BstF5I GGATG 8 cut(s) 172, 686, 1224, 1666, 2557, 2590, 2728, 2761
BstFNI CGCG 1 cut(s) 1357
BstH2I RGCGCY 1 cut(s) 1821
BstHHI GCGC 2 cut(s) 1250, 1820
BstMAI GTCTC 5 cut(s) 65, 123, 1367, 1600, 1833
BstNI CCWGG 2 cut(s) 2066, 2562
BstNSI RCATGY 5 cut(s) 518, 941, 1088, 2429, 2441
BstPAI GACNNNNGTC 1 cut(s) 2573
BstSCI CCNGG 3 cut(s) 1552, 2064, 2560
BstSFI CTRYAG 2 cut(s) 393, 2647
BstSLI GKGCMC 1 cut(s) 1644
BstSNI TACGTA 1 cut(s) 2337
BstUI CGCG 1 cut(s) 1357
BstV2I GAAGAC 2 cut(s) 288, 528
BstX2I RGATCY 3 cut(s) 584, 2236, 2783
BstYI RGATCY 3 cut(s) 584, 2236, 2783
BstZ17I GTATAC 1 cut(s) 2340
BsuRI GGCC 3 cut(s) 972, 1013, 1642
BtgI CCRYGG 1 cut(s) 124
BtsCI GGATG 8 cut(s) 172, 686, 1224, 1666, 2557, 2590, 2728, 2761
BtsI GCAGTG 3 cut(s) 1044, 2394, 2644
BtsIMutI CAGTG 6 cut(s) 697, 838, 1044, 1769, 2394, 2644
Cac8I GCNNGC 6 cut(s) 426, 1073, 1328, 1567, 1585, 2427
CciI TCATGA 2 cut(s) 142, 2512
CfoI GCGC 2 cut(s) 1250, 1820
Cfr10I RCCGGY 2 cut(s) 718, 1071
Cfr13I GGNCC 6 cut(s) 446, 1012, 1285, 1640, 1641, 2506
CseI GACGC 1 cut(s) 884
CsiI ACCWGGT 1 cut(s) 2064
Csp6I GTAC 3 cut(s) 590, 2279, 2582
CspAI ACCGGT 1 cut(s) 718
CspCI CAANNNNNGTGG 2 cut(s) 1758, 1793
CviQI GTAC 3 cut(s) 590, 2279, 2582
DraIII CACNNNGTG 1 cut(s) 2455
EaeI YGGCCR 1 cut(s) 970
Eam1104I CTCTTC 3 cut(s) 1542, 1986, 2782
EarI CTCTTC 3 cut(s) 1542, 1986, 2782
Ecl136II GAGCTC 1 cut(s) 2608
Eco105I TACGTA 1 cut(s) 2337
Eco130I CCWWGG 4 cut(s) 36, 124, 276, 2050
Eco24I GRGCYC 2 cut(s) 1644, 2610
Eco31I GGTCTC 1 cut(s) 1600
Eco47I GGWCC 3 cut(s) 446, 1285, 2506
Eco53kI GAGCTC 1 cut(s) 2608
Eco57I CTGAAG 2 cut(s) 1113, 1352
Eco88I CYCGRG 1 cut(s) 2229
EcoICRI GAGCTC 1 cut(s) 2608
EcoO109I RGGNCCY 2 cut(s) 1640, 1641
EcoRI GAATTC 1 cut(s) 1234
EcoRII CCWGG 2 cut(s) 2064, 2560
EcoT14I CCWWGG 4 cut(s) 36, 124, 276, 2050
EcoT38I GRGCYC 2 cut(s) 1644, 2610
ErhI CCWWGG 4 cut(s) 36, 124, 276, 2050
FaqI GGGAC 2 cut(s) 721, 2581
FbaI TGATCA 2 cut(s) 2551, 2736
FblI GTMKAC 1 cut(s) 2339
FokI GGATG 8 cut(s) 179, 673, 1211, 1653, 2544, 2577, 2735, 2768
FriOI GRGCYC 2 cut(s) 1644, 2610
FspBI CTAG 6 cut(s) 92, 906, 1178, 1212, 1566, 2088
GlaI GCGC 2 cut(s) 1249, 1819
GsuI CTGGAG 4 cut(s) 2175, 2200, 2478, 2583
HaeII RGCGCY 1 cut(s) 1821
HaeIII GGCC 3 cut(s) 972, 1013, 1642
HapII CCGG 7 cut(s) 719, 973, 1072, 1554, 1593, 2363, 2531
HgaI GACGC 1 cut(s) 884
HhaI GCGC 2 cut(s) 1250, 1820
Hin6I GCGC 2 cut(s) 1248, 1818
HinP1I GCGC 2 cut(s) 1248, 1818
HincII GTYRAC 1 cut(s) 2314
HindII GTYRAC 1 cut(s) 2314
HindIII AAGCTT 3 cut(s) 1217, 1328, 2198
HpaI GTTAAC 1 cut(s) 2314
HpaII CCGG 7 cut(s) 719, 973, 1072, 1554, 1593, 2363, 2531
HphI GGTGA 1 cut(s) 875
Hpy166II GTNNAC 9 cut(s) 413, 1082, 1337, 2279, 2281, 2314, 2340, 2399, 2582
Hpy8I GTNNAC 9 cut(s) 413, 1082, 1337, 2279, 2281, 2314, 2340, 2399, 2582
HpyAV CCTTC 8 cut(s) 295, 772, 1113, 1590, 1620, 1631, 1926, 2024
HpyCH4III ACNGT 2 cut(s) 1302, 2414
HpyCH4IV ACGT 3 cut(s) 2336, 2342, 2451
HpySE526I ACGT 3 cut(s) 2336, 2342, 2451
HspAI GCGC 2 cut(s) 1248, 1818
Kpn2I TCCGGA 1 cut(s) 2530
KroI GCCGGC 1 cut(s) 1071
KroNI GCCGGC 1 cut(s) 1073
Ksp22I TGATCA 2 cut(s) 2551, 2736
KspAI GTTAAC 1 cut(s) 2314
LmnI GCTCC 5 cut(s) 13, 1028, 2194, 2497, 2605
LweI GCATC 7 cut(s) 25, 186, 1702, 2441, 2566, 2713, 2746
MabI ACCWGGT 1 cut(s) 2064
MaeI CTAG 6 cut(s) 92, 906, 1178, 1212, 1566, 2088
MaeII ACGT 3 cut(s) 2336, 2342, 2451
MaeIII GTNAC 8 cut(s) 863, 1040, 1418, 1531, 2068, 2248, 2343, 2474
MfeI CAATTG 3 cut(s) 1251, 1692, 1941
MflI RGATCY 3 cut(s) 584, 2236, 2783
MhlI GDGCHC 5 cut(s) 430, 535, 760, 1644, 2610
MluI ACGCGT 1 cut(s) 1355
MlyI GAGTC 3 cut(s) 644, 1403, 2768
MmeI TCCRAC 3 cut(s) 797, 1498, 2229
MroI TCCGGA 1 cut(s) 2530
MroNI GCCGGC 1 cut(s) 1071
MroXI GAANNNNTTC 3 cut(s) 285, 1365, 1796
MseI TTAA 9 cut(s) 245, 336, 842, 1461, 1811, 1833, 2313, 2382, 2742
MslI CAYNNNNRTG 6 cut(s) 296, 1089, 1356, 1527, 1710, 2721
MspI CCGG 7 cut(s) 719, 973, 1072, 1554, 1593, 2363, 2531
MspR9I CCNGG 3 cut(s) 1554, 2066, 2562
MunI CAATTG 3 cut(s) 1251, 1692, 1941
Mva1269I GAATGC 4 cut(s) 67, 110, 1631, 2676
MvaI CCWGG 2 cut(s) 2066, 2562
MvnI CGCG 1 cut(s) 1357
NaeI GCCGGC 1 cut(s) 1073
NciI CCSGG 1 cut(s) 1554
NcoI CCATGG 1 cut(s) 124
NgoMIV GCCGGC 1 cut(s) 1071
NheI GCTAGC 1 cut(s) 1565
NlaIV GGNNCC 7 cut(s) 1642, 1643, 2105, 2361, 2367, 2493, 2508
NmeAIII GCCGAG 1 cut(s) 89
NmuCI GTSAC 4 cut(s) 863, 1040, 2068, 2343
NspI RCATGY 5 cut(s) 518, 941, 1088, 2429, 2441
NspV TTCGAA 2 cut(s) 155, 1635
PaeI GCATGC 1 cut(s) 2429
PagI TCATGA 2 cut(s) 142, 2512
PciI ACATGT 1 cut(s) 1084
PctI GAATGC 4 cut(s) 67, 110, 1631, 2676
PdiI GCCGGC 1 cut(s) 1073
PdmI GAANNNNTTC 3 cut(s) 285, 1365, 1796
PfeI GAWTC 9 cut(s) 146, 374, 606, 828, 1186, 1871, 2268, 2466, 2539
PflFI GACNNNGTC 1 cut(s) 894
PfoI TCCNGGA 2 cut(s) 1552, 2560
PinAI ACCGGT 1 cut(s) 718
PleI GAGTC 3 cut(s) 643, 1402, 2768
PpsI GAGTC 3 cut(s) 643, 1402, 2768
Ppu21I YACGTR 1 cut(s) 2337
PscI ACATGT 1 cut(s) 1084
PshAI GACNNNNGTC 1 cut(s) 2573
PsiI TTATAA 1 cut(s) 1023
Psp124BI GAGCTC 1 cut(s) 2610
Psp6I CCWGG 2 cut(s) 2064, 2560
PspGI CCWGG 2 cut(s) 2064, 2560
PspN4I GGNNCC 7 cut(s) 1642, 1643, 2105, 2361, 2367, 2493, 2508
PspOMI GGGCCC 1 cut(s) 1640
PspPI GGNCC 6 cut(s) 446, 1012, 1285, 1640, 1641, 2506
PstI CTGCAG 2 cut(s) 397, 2651
PsuI RGATCY 3 cut(s) 584, 2236, 2783
PsyI GACNNNGTC 1 cut(s) 894
RsaI GTAC 3 cut(s) 591, 2280, 2583
RsaNI GTAC 3 cut(s) 590, 2279, 2582
RseI CAYNNNNRTG 6 cut(s) 296, 1089, 1356, 1527, 1710, 2721
SacI GAGCTC 1 cut(s) 2610
SaqAI TTAA 9 cut(s) 245, 336, 842, 1461, 1811, 1833, 2313, 2382, 2742
Sau96I GGNCC 6 cut(s) 446, 1012, 1285, 1640, 1641, 2506
SchI GAGTC 3 cut(s) 644, 1403, 2768
ScrFI CCNGG 3 cut(s) 1554, 2066, 2562
SduI GDGCHC 5 cut(s) 430, 535, 760, 1644, 2610
SexAI ACCWGGT 1 cut(s) 2064
SfaNI GCATC 7 cut(s) 25, 186, 1702, 2441, 2566, 2713, 2746
SfcI CTRYAG 2 cut(s) 393, 2647
SfuI TTCGAA 2 cut(s) 155, 1635
SinI GGWCC 3 cut(s) 446, 1285, 2506
SmiMI CAYNNNNRTG 6 cut(s) 296, 1089, 1356, 1527, 1710, 2721
SmlI CTYRAG 1 cut(s) 789
SmoI CTYRAG 1 cut(s) 789
SnaBI TACGTA 1 cut(s) 2337
SpeI ACTAGT 1 cut(s) 91
SphI GCATGC 1 cut(s) 2429
SsiI CCGC 2 cut(s) 309, 2504
SspI AATATT 1 cut(s) 1655
SspMI CTAG 6 cut(s) 92, 906, 1178, 1212, 1566, 2088
SstI GAGCTC 1 cut(s) 2610
StyD4I CCNGG 3 cut(s) 1552, 2064, 2560
StyI CCWWGG 4 cut(s) 36, 124, 276, 2050
TaaI ACNGT 2 cut(s) 1302, 2414
TaiI ACGT 3 cut(s) 2339, 2345, 2454
TaqI TCGA 7 cut(s) 155, 519, 535, 638, 1635, 1936, 1989
TatI WGTACW 3 cut(s) 589, 2278, 2581
TfiI GAWTC 9 cut(s) 146, 374, 606, 828, 1186, 1871, 2268, 2466, 2539
Tru1I TTAA 9 cut(s) 245, 336, 842, 1461, 1811, 1833, 2313, 2382, 2742
Tru9I TTAA 9 cut(s) 245, 336, 842, 1461, 1811, 1833, 2313, 2382, 2742
TscAI CASTG 6 cut(s) 697, 838, 1051, 1776, 2394, 2651
TseFI GTSAC 4 cut(s) 863, 1040, 2068, 2343
Tsp45I GTSAC 4 cut(s) 863, 1040, 2068, 2343
TspGWI ACGGA 1 cut(s) 1041
TspRI CASTG 6 cut(s) 697, 838, 1051, 1776, 2394, 2651
Tth111I GACNNNGTC 1 cut(s) 894
VpaK11BI GGWCC 3 cut(s) 446, 1285, 2506
XapI RAATTY 4 cut(s) 965, 1165, 1234, 1362
XbaI TCTAGA 1 cut(s) 1177
XceI RCATGY 5 cut(s) 518, 941, 1088, 2429, 2441
XcmI CCANNNNNNNNNTGG 1 cut(s) 965
XmiI GTMKAC 1 cut(s) 2339
XmnI GAANNNNTTC 3 cut(s) 285, 1365, 1796
XspI CTAG 6 cut(s) 92, 906, 1178, 1212, 1566, 2088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.