Rw7G005000

ATP-dependent helicase hrq1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0006845
Physical Location & Seq
Reverse (-)
4189231 .. 4197052
7822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G005000.1

Sequence Viewer

Length: 3627 bp
ATGGCAGCGAGTCGGATTCAAATCGAGGTCCGTACATTGACCGGCGAAGCGACCACCGTCTCAGCCTCGGTCAACGACGCCGTTTCAGACCTCAAGCTACTTCTCAACCAGTCCTTCCCTCCCGCCACCAACTCTCCCAATTTCCACCTCTTCTTCAAGGGTGAGAAGTTGAGGTTGCAGAGTCGAATAGGCACGCACGGGATTAAGCCGGGTGAGGTTCTAGTGCTGGTCCCGTTTGTTAAAAAGGTGGCCAATAATCAGAGCCAGAAATGTGATCAATCTGATCCATCGTTGAATACTTCTGCTAATCGCTCCATGTCCAAGTTTGCGAACTCCGCATGGTCTGATATGATGCAGGACTTGTCATATTTGCGTGATGGTTCGAGTGATGGAACCCCCAGTGTGTCTAATATAGGGAGTTTTAGTGTTGGAGATGGAAGTGGAGATTGCTCTTGTGAAGTGAAGCGGAAGAGGGGGTTTGGTTGTGATGAGATGATATTGGATATGTTGTGGGCTAGTAGAAGCAAGAGTGTTCTTGATGAACAGAATTCTAGAAGGTTTGTTGAGCTTTTGGAGTCGGTGAATTGTTTATCGGATCCATATTCTGGGGACTGCATGTTGTGGAGAAGAAAAGCTAGCTTACAAGGCTTTGGCTTGGGGCTGCCTAACGGCAATGGTAATTCATGCTTGTGTCCAGAGTGGTTGAAGAAAATCATGAAGGCATTTGCTTTCTTAAACATTTTTTCTGCACTTGTTCAACTTCAGCAGGAGAGGACAACCTCAACTCTATTGGAGCAAGCACTGGAACAGCTTGCGACATTTGGAGTTAAACTTGATATGCAGGACATGGAGCATCTTTCGGTTATTTCTCCCAAGGTAGTTCTTTTTGTGAAGAAGAATGCAGAGGAGACAAGTTCTGGTAATGCACTTGTTATTATTGAATGCTCGGCAGAACAGAACCATGGAGACAGAAATAATCATGAATCTGCTTGCAAACAGATGGATGTATCAAAGATTGTCAATGAATTGAAGATGCAGGAAAGTTCTTTCAAAAGTAATTTGTGGGAGGCTGTTGAGAGGTTAATGTTTATCAAAAGAAATGAGATTTCCTCCTTGGAAGACTTTCTCATTTCTGTGAAGGATTGCGGTGCTGCTGCAAGAGGAAATAAAGTTAATCAAGCAACAAAAAGCCACACAGCTTCAACTACTGATTCAGACAAAAAGCACTCTGCAGCTTCAACCTCTCGTTCACACAAAAAGCGAGCACTATGTCATAGAACAGGTCCATTGCTACCAGTTGAGATGGTTGAACATCTTAGAAAGAGAATTGGATCAGCAACGAAGATAGTGCATGTCGAAGACATTGGTGCTCGAAATGCCGTTCATGTGGAAGTTCCACAGGAACTATCAGAAAATCTGAGATCTGTACTTCATTCTATTGGAATCACTCAGTTATATAGCCATCAGGCAGAGTCGATACAAGCCTCACTAACTGGCAAGAATGTTGTTGTGGCTACAATGACATCCAGTGGGAAATCTCTTTGCTACAATCTACCGGTCATTGAAGCCCTGTCCCAGAGTTCCTCATCATGTGCTCTGTACTTATTTCCAACGAAGGCTTTAGCTCAAGATCAACTAAGAGCTTTCTTAGCTATGACCAAAGGATTCAGTGGTAGCTTAAATGTTGGTATATATGATGGTGACACTACTCAGCAGGAAAGGACACGACTGCGTCAAAATGCTAGACTGCTAATCACAAATCCAGATATGTTGCATGTATCAATCCTGCCACACCACCGAAAATTTGGCCGGATTTTGGCAAATCTTAGGTATGTAGTGATTGATGAGGCCCATGTTTATAAGGGAGCATTTGGTTGTCACACTGCTCTTATACTTAGGCGACTTCGCCGGCTCTGTTCACATGTGTATGGAAGTGATCCTTCTTTTGTGTTTTCTACTGCGACTTCGGCAAATCCTCGTGAGCATTGCATGGAACTTGCGAATTTACCAACTCTAGAGTTGATTCAGAATGATGGAAGCCCTGCTGCTAGAAAGCTTTTCATCCTTTGGAATTCTGAGGACAGCGCAATTGAGGATATATCATTCCTCTTTGCTGAAATGGTCCAGCATGGACTACGGTGCATTGCATTTTGCAAAACACGCAAGCTTTGTGAACTTGTTTTTTGCCATACGCGTGAAATTCTTCAGGAGACAGCACCCCATCTGGCAGAGTCTATATGTTCTTATCGTGCTGGTTACATTGCTGAGGATAGGAGAAGAATAGAGAGTGATTTATTTAACGGGAAACTTTCTGGTATTGCTGCAACAAATGCCCTTGAATTGGGTATTGATGTCGGACACATTGATGTAACTCTTCATTTGGGTTTTCCCGGAAGTATTGCTAGCTTGTGGCAACAAGCTGGCAGAGCCGGAAGGAGAGAGAGACCTTCTCTTGCTGTATATGTTGCATTCGAAGGGCCCCTTGACCAATATTTTATGAAACATCCAAATAAACTTTTTGGAAGCCCAATTGAGTGCTGTCATGTTGATGCTAAGAACCAACAGGTTCTTGAACAGCATTTGGCTTGTGCTGCTCTTGAACACCCACTGAGCTTGTTTTATGATGGGAAGTTTTTCGGTTCTGGCTTAAACAGCGCCATAATGTCTCTTAAAAGTAGAGGATCTCTGAGTTATGATCCATCATGTGATTCTTCTGCTAAAATCTGGAACTATATTGGGTATCAGAAAATGCCTTCACATTCAGTTAGTATTCGATCAATTGAAAGAGAGAGATACAAAGTTATGGACCATCAAGAAAAAGAAATCGAAGAGATTGAGGAAAGCAAGGCTTTCTTTCAGGTATATGAAGGTGCTGTTTATTTGAACCAAGGAAAGACATACCTGGTCACGAGCTTAGATTTATCTAGCAAGATTGCTTGGTGCCAAAAAGCTGATGTAAATTATTATACGAAACCCCGTGATCGCACAGATATTCATGTAGTTGGTGGTAATGTGGCTTATCCAGCTCCAGCTAAGCTTTCCAACCAGTTCTCCAGAACAACTGCCCGAGCAGATCCTTGCAGAGTAACAACTACATGGTTTGGATTCTATCGTGTACACAAAGGAAGCAACATGATATTTGATAAAGTTAAACTTGCACTTCCTAAATATACGTATACGTCACAGGCAGTTTGGGTTCCGGTTCCACGATCAATAATTAAAGCAGTGGAGAGTAAACAACTTGAATACCGTGCAGGGCTGCATGCTGCATCACATGCTGTTCTGCACGTTGTGCCTCTACGAATCATTTGTAACTTATCTGACTTGGCTCCAGAGTGTGCGGACCCTCATGACGGCCGCTATTTTCCGGAGAGGATTCTATTATATGATCAGCATCCTGGAGGGACTGGTGTCTGTGTACAGGTTCATCCGTTTTTTATGGAGCTCTTGGCTACTGCTCTGGAACTCCTGACATCTTGCCACTGCAGAGAAGATGATGGTTGCCCTAATTGCATTCAGAGTTTTTGTTGCAAGTCATATAATGAGGTTTTACACAAGGATGCAGCAATTATGATCATTAAGGGTATTCTGGATGCGAATATATCTTCTGACTCTTCTGGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DUF1998 Helicase_C

Protein Analysis

1208

Amino Acids

134.02

Weight (kDa)

8.02

Isoelectric Point (pI)

47.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 484 - 621 7.4e-07 Type III restriction enzyme, res subunit
DEAD PF00270 487 - 657 6.6e-31 DEAD/DEAH box helicase
Helicase_C PF00271 707 - 813 1.6e-12 Helicase conserved C-terminal domain
WHD_HRQ1 PF22982 849 - 927 2.7e-16 ATP-dependent helicase HRQ1, winged helix domain
MZB PF09369 1090 - 1172 7.9e-18 MrfA Zn-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1860
AccB1I GGYRCC 1 cut(s) 2940
AccB7I CCANNNNNTGG 1 cut(s) 605
AccI GTMKAC 1 cut(s) 3176
AccII CGCG 1 cut(s) 2194
AccIII TCCGGA 1 cut(s) 3367
AciI CCGC 6 cut(s) 123, 336, 466, 1146, 3341, 3358
AclWI GGATC 8 cut(s) 278, 590, 603, 1339, 1931, 2689, 2690, 3068
AcoI YGGCCR 3 cut(s) 249, 1807, 3355
AcsI RAATTY 5 cut(s) 547, 1802, 2002, 2071, 2199
AcuI CTGAAG 2 cut(s) 746, 2189
AcyI GRCGYC 1 cut(s) 78
AdeI CACNNNGTG 1 cut(s) 3292
AfaI GTAC 5 cut(s) 34, 1428, 1601, 3117, 3420
AfiI CCNNNNNNNGG 4 cut(s) 605, 1816, 2341, 3353
AflIII ACRYGT 2 cut(s) 1921, 2192
AgeI ACCGGT 1 cut(s) 1555
AjnI CCWGG 2 cut(s) 2901, 3397
Alw21I GWGCWC 4 cut(s) 1267, 1372, 1597, 3447
Alw26I GTCTC 6 cut(s) 64, 902, 960, 2204, 2437, 2670
AlwI GGATC 8 cut(s) 278, 590, 603, 1339, 1931, 2689, 2690, 3068
Ama87I CYCGRG 1 cut(s) 3066
Aor13HI TCCGGA 1 cut(s) 3367
AoxI GGCC 5 cut(s) 249, 1807, 1848, 2477, 3355
ApaI GGGCCC 1 cut(s) 2481
ApoI RAATTY 5 cut(s) 547, 1802, 2002, 2071, 2199
AsiGI ACCGGT 1 cut(s) 1555
Asp700I GAANNNNTTC 3 cut(s) 1122, 2202, 2633
AspLEI GCGC 2 cut(s) 2087, 2657
AspS9I GGNCC 9 cut(s) 28, 229, 1283, 1849, 2122, 2477, 2478, 2806, 3343
AsuC2I CCSGG 2 cut(s) 210, 2391
AsuHPI GGTGA 4 cut(s) 173, 224, 592, 1712
AsuII TTCGAA 1 cut(s) 2472
AsuNHI GCTAGC 2 cut(s) 635, 2402
AvaI CYCGRG 1 cut(s) 3066
AvaII GGWCC 6 cut(s) 28, 229, 1283, 2122, 2806, 3343
BaeGI GKGCMC 1 cut(s) 2481
BalI TGGCCA 1 cut(s) 251
BamHI GGATCC 1 cut(s) 595
BanI GGYRCC 1 cut(s) 2940
BanII GRGCYC 2 cut(s) 2481, 3447
BauI CACGAG 2 cut(s) 1977, 2908
BbsI GAAGAC 2 cut(s) 1125, 1365
Bbv12I GWGCWC 4 cut(s) 1267, 1372, 1597, 3447
BbvCI CCTCAGC 1 cut(s) 2265
BceAI ACGGC 4 cut(s) 65, 685, 1364, 3370
BcgI CGANNNNNNTGC 2 cut(s) 1330, 1364
BciT130I CCWGG 2 cut(s) 2903, 3399
BclI TGATCA 3 cut(s) 274, 3388, 3573
BcnI CCSGG 2 cut(s) 210, 2391
BcoDI GTCTC 6 cut(s) 64, 902, 960, 2204, 2437, 2670
BfaI CTAG 9 cut(s) 221, 516, 552, 636, 1743, 2015, 2049, 2403, 2925
BfmI CTRYAG 2 cut(s) 1230, 3484
BfoI RGCGCY 1 cut(s) 2658
BglII AGATCT 1 cut(s) 1421
BlpI GCTNAGC 1 cut(s) 3033
Bme1390I CCNGG 4 cut(s) 210, 2391, 2903, 3399
Bme18I GGWCC 6 cut(s) 28, 229, 1283, 2122, 2806, 3343
BmeT110I CYCGRG 1 cut(s) 3066
BmgT120I GGNCC 9 cut(s) 28, 229, 1283, 1849, 2122, 2477, 2478, 2806, 3343
BmrFI CCNGG 4 cut(s) 210, 2391, 2903, 3399
BmrI ACTGGG 1 cut(s) 393
BmsI GCATC 8 cut(s) 342, 862, 1023, 2539, 3278, 3403, 3550, 3583
BmtI GCTAGC 2 cut(s) 639, 2406
BmuI ACTGGG 1 cut(s) 393
BoxI GACNNNNGTC 2 cut(s) 56, 3410
BpiI GAAGAC 2 cut(s) 1125, 1365
BplI GAGNNNNNCTC 4 cut(s) 1094, 1126, 2434, 2466
BpmI CTGGAG 4 cut(s) 3012, 3037, 3315, 3420
Bpu10I CCTNAGC 1 cut(s) 2265
Bpu1102I GCTNAGC 1 cut(s) 3033
Bpu14I TTCGAA 1 cut(s) 2472
BpuEI CTTGAG 2 cut(s) 77, 1611
BpuMI CCSGG 2 cut(s) 210, 2391
BsaAI YACGTR 1 cut(s) 3174
BsaBI GATNNNNATC 2 cut(s) 20, 3393
BsaHI GRCGYC 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 2437
BsaJI CCNNGG 5 cut(s) 66, 873, 961, 1113, 2887
BsaWI WCCGGW 3 cut(s) 1555, 3199, 3367
BsaXI ACNNNNNCTCC 6 cut(s) 118, 148, 409, 439, 3035, 3065
Bsc4I CCNNNNNNNGG 4 cut(s) 605, 1816, 2341, 3353
Bse118I RCCGGY 3 cut(s) 41, 1555, 1908
Bse1I ACTGG 8 cut(s) 109, 399, 807, 1295, 1498, 1527, 3046, 3412
Bse3DI GCAATG 5 cut(s) 679, 1286, 1984, 2142, 2259
Bse8I GATNNNNATC 2 cut(s) 20, 3393
BseAI TCCGGA 1 cut(s) 3367
BseBI CCWGG 2 cut(s) 2903, 3399
BseDI CCNNGG 5 cut(s) 66, 873, 961, 1113, 2887
BseGI GGATG 8 cut(s) 1009, 1523, 2061, 2503, 3394, 3427, 3565, 3598
BseJI GATNNNNATC 2 cut(s) 20, 3393
BseLI CCNNNNNNNGG 4 cut(s) 605, 1816, 2341, 3353
BseMI GCAATG 5 cut(s) 679, 1286, 1984, 2142, 2259
BseMII CTCAG 8 cut(s) 75, 1409, 1463, 1724, 2067, 2256, 2600, 2678
BseNI ACTGG 8 cut(s) 109, 399, 807, 1295, 1498, 1527, 3046, 3412
BseRI GAGGAG 1 cut(s) 920
BseSI GKGCMC 1 cut(s) 2481
BseX3I CGGCCG 1 cut(s) 3355
BsgI GTGCAG 3 cut(s) 732, 3269, 3273
Bsh1236I CGCG 1 cut(s) 2194
Bsh1285I CGRYCG 1 cut(s) 3358
BshFI GGCC 5 cut(s) 251, 1809, 1850, 2479, 3357
BshNI GGYRCC 1 cut(s) 2940
BshTI ACCGGT 1 cut(s) 1555
BsiEI CGRYCG 1 cut(s) 3358
BsiHKAI GWGCWC 4 cut(s) 1267, 1372, 1597, 3447
BsiHKCI CYCGRG 1 cut(s) 3066
BsiSI CCGG 9 cut(s) 42, 209, 1556, 1810, 1909, 2391, 2430, 3200, 3368
BslFI GGGAC 4 cut(s) 215, 623, 1558, 3418
BslI CCNNNNNNNGG 4 cut(s) 605, 1816, 2341, 3353
BsmAI GTCTC 6 cut(s) 64, 902, 960, 2204, 2437, 2670
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 4 cut(s) 215, 623, 1558, 3418
BsmI GAATGC 4 cut(s) 904, 947, 2468, 3513
BsnI GGCC 5 cut(s) 251, 1809, 1850, 2479, 3357
Bso31I GGTCTC 1 cut(s) 2437
BsoBI CYCGRG 1 cut(s) 3066
Bsp119I TTCGAA 1 cut(s) 2472
Bsp120I GGGCCC 1 cut(s) 2477
Bsp1286I GDGCHC 5 cut(s) 1267, 1372, 1597, 2481, 3447
Bsp13I TCCGGA 1 cut(s) 3367
Bsp1407I TGTACA 2 cut(s) 3115, 3418
Bsp1720I GCTNAGC 1 cut(s) 3033
Bsp19I CCATGG 1 cut(s) 961
BspACI CCGC 6 cut(s) 123, 336, 466, 1146, 3341, 3358
BspANI GGCC 5 cut(s) 251, 1809, 1850, 2479, 3357
BspCNI CTCAG 8 cut(s) 74, 1410, 1462, 1723, 2068, 2257, 2601, 2679
BspEI TCCGGA 1 cut(s) 3367
BspFNI CGCG 1 cut(s) 2194
BspHI TCATGA 3 cut(s) 714, 979, 3349
BspMAI CTGCAG 2 cut(s) 1234, 3488
BspOI GCTAGC 2 cut(s) 639, 2406
BspPI GGATC 8 cut(s) 278, 590, 603, 1339, 1931, 2689, 2690, 3068
BspT104I TTCGAA 1 cut(s) 2472
BspT107I GGYRCC 1 cut(s) 2940
BspTNI GGTCTC 1 cut(s) 2437
BsrDI GCAATG 5 cut(s) 679, 1286, 1984, 2142, 2259
BsrFI RCCGGY 3 cut(s) 41, 1555, 1908
BsrGI TGTACA 2 cut(s) 3115, 3418
BsrI ACTGG 8 cut(s) 109, 399, 807, 1295, 1498, 1527, 3046, 3412
BssAI RCCGGY 3 cut(s) 41, 1555, 1908
BssECI CCNNGG 5 cut(s) 66, 873, 961, 1113, 2887
BssNAI GTATAC 1 cut(s) 3177
BssNI GRCGYC 1 cut(s) 78
BssSI CACGAG 2 cut(s) 1977, 2908
BssT1I CCWWGG 4 cut(s) 873, 961, 1113, 2887
Bst1107I GTATAC 1 cut(s) 3177
Bst2BI CACGAG 2 cut(s) 1977, 2908
Bst2UI CCWGG 2 cut(s) 2903, 3399
Bst4CI ACNGT 3 cut(s) 58, 2139, 3251
Bst6I CTCTTC 5 cut(s) 155, 464, 2379, 2823, 3619
BstACI GRCGYC 1 cut(s) 78
BstAPI GCANNNNNTGC 3 cut(s) 2330, 3275, 3292
BstAUI TGTACA 2 cut(s) 3115, 3418
BstBAI YACGTR 1 cut(s) 3174
BstBI TTCGAA 1 cut(s) 2472
BstDSI CCRYGG 1 cut(s) 961
BstF5I GGATG 8 cut(s) 1009, 1523, 2061, 2503, 3394, 3427, 3565, 3598
BstFNI CGCG 1 cut(s) 2194
BstH2I RGCGCY 1 cut(s) 2658
BstHHI GCGC 2 cut(s) 2087, 2657
BstMAI GTCTC 6 cut(s) 64, 902, 960, 2204, 2437, 2670
BstMCI CGRYCG 1 cut(s) 3358
BstNI CCWGG 2 cut(s) 2903, 3399
BstNSI RCATGY 6 cut(s) 619, 1355, 1778, 1925, 3266, 3278
BstPAI GACNNNNGTC 2 cut(s) 56, 3410
BstSCI CCNGG 4 cut(s) 208, 2389, 2901, 3397
BstSFI CTRYAG 2 cut(s) 1230, 3484
BstSLI GKGCMC 1 cut(s) 2481
BstSNI TACGTA 1 cut(s) 3174
BstUI CGCG 1 cut(s) 2194
BstV2I GAAGAC 2 cut(s) 1125, 1365
BstX2I RGATCY 5 cut(s) 595, 1421, 2681, 3073, 3620
BstYI RGATCY 5 cut(s) 595, 1421, 2681, 3073, 3620
BstZ17I GTATAC 1 cut(s) 3177
BstZI CGGCCG 1 cut(s) 3355
BsuRI GGCC 5 cut(s) 251, 1809, 1850, 2479, 3357
BtgI CCRYGG 1 cut(s) 961
BtsCI GGATG 8 cut(s) 1009, 1523, 2061, 2503, 3394, 3427, 3565, 3598
BtsI GCAGTG 3 cut(s) 1881, 3231, 3481
BtsIMutI CAGTG 8 cut(s) 406, 800, 1534, 1675, 1881, 2606, 3231, 3481
CciI TCATGA 3 cut(s) 714, 979, 3349
CfoI GCGC 2 cut(s) 2087, 2657
Cfr10I RCCGGY 3 cut(s) 41, 1555, 1908
Cfr13I GGNCC 9 cut(s) 28, 229, 1283, 1849, 2122, 2477, 2478, 2806, 3343
CseI GACGC 2 cut(s) 86, 1721
CsiI ACCWGGT 1 cut(s) 2901
Csp6I GTAC 5 cut(s) 33, 1427, 1600, 3116, 3419
CspAI ACCGGT 1 cut(s) 1555
CspCI CAANNNNNGTGG 2 cut(s) 2595, 2630
CviQI GTAC 5 cut(s) 33, 1427, 1600, 3116, 3419
DraIII CACNNNGTG 1 cut(s) 3292
EaeI YGGCCR 3 cut(s) 249, 1807, 3355
EagI CGGCCG 1 cut(s) 3355
Eam1104I CTCTTC 5 cut(s) 155, 464, 2379, 2823, 3619
EarI CTCTTC 5 cut(s) 155, 464, 2379, 2823, 3619
Ecl136II GAGCTC 1 cut(s) 3445
EclXI CGGCCG 1 cut(s) 3355
Eco105I TACGTA 1 cut(s) 3174
Eco130I CCWWGG 4 cut(s) 873, 961, 1113, 2887
Eco24I GRGCYC 2 cut(s) 2481, 3447
Eco31I GGTCTC 1 cut(s) 2437
Eco47I GGWCC 6 cut(s) 28, 229, 1283, 2122, 2806, 3343
Eco52I CGGCCG 1 cut(s) 3355
Eco53kI GAGCTC 1 cut(s) 3445
Eco57I CTGAAG 2 cut(s) 746, 2189
Eco88I CYCGRG 1 cut(s) 3066
EcoICRI GAGCTC 1 cut(s) 3445
EcoO109I RGGNCCY 2 cut(s) 2477, 2478
EcoRI GAATTC 2 cut(s) 547, 2071
EcoRII CCWGG 2 cut(s) 2901, 3397
EcoT14I CCWWGG 4 cut(s) 873, 961, 1113, 2887
EcoT38I GRGCYC 2 cut(s) 2481, 3447
ErhI CCWWGG 4 cut(s) 873, 961, 1113, 2887
Esp3I CGTCTC 1 cut(s) 64
FaqI GGGAC 4 cut(s) 215, 623, 1558, 3418
FauI CCCGC 1 cut(s) 130
FbaI TGATCA 3 cut(s) 274, 3388, 3573
FblI GTMKAC 1 cut(s) 3176
FokI GGATG 8 cut(s) 1016, 1510, 2048, 2490, 3381, 3414, 3572, 3605
FriOI GRGCYC 2 cut(s) 2481, 3447
FspBI CTAG 9 cut(s) 221, 516, 552, 636, 1743, 2015, 2049, 2403, 2925
GlaI GCGC 2 cut(s) 2086, 2656
GsuI CTGGAG 4 cut(s) 3012, 3037, 3315, 3420
HaeII RGCGCY 1 cut(s) 2658
HaeIII GGCC 5 cut(s) 251, 1809, 1850, 2479, 3357
HapII CCGG 9 cut(s) 42, 209, 1556, 1810, 1909, 2391, 2430, 3200, 3368
HgaI GACGC 2 cut(s) 86, 1721
HhaI GCGC 2 cut(s) 2087, 2657
Hin1I GRCGYC 1 cut(s) 78
Hin6I GCGC 2 cut(s) 2085, 2655
HinP1I GCGC 2 cut(s) 2085, 2655
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HindIII AAGCTT 3 cut(s) 2054, 2165, 3035
HpaII CCGG 9 cut(s) 42, 209, 1556, 1810, 1909, 2391, 2430, 3200, 3368
HphI GGTGA 4 cut(s) 173, 224, 592, 1712
Hpy166II GTNNAC 9 cut(s) 73, 1250, 1919, 2174, 3116, 3118, 3177, 3236, 3419
Hpy8I GTNNAC 9 cut(s) 73, 1250, 1919, 2174, 3116, 3118, 3177, 3236, 3419
Hpy99I CGWCG 1 cut(s) 80
HpyCH4III ACNGT 3 cut(s) 58, 2139, 3251
HpyCH4IV ACGT 3 cut(s) 3173, 3179, 3288
HpySE526I ACGT 3 cut(s) 3173, 3179, 3288
Hsp92I GRCGYC 1 cut(s) 78
HspAI GCGC 2 cut(s) 2085, 2655
Kpn2I TCCGGA 1 cut(s) 3367
KroI GCCGGC 1 cut(s) 1908
KroNI GCCGGC 1 cut(s) 1910
Ksp22I TGATCA 3 cut(s) 274, 3388, 3573
LmnI GCTCC 7 cut(s) 317, 793, 850, 1865, 3031, 3334, 3442
LweI GCATC 8 cut(s) 342, 862, 1023, 2539, 3278, 3403, 3550, 3583
MabI ACCWGGT 1 cut(s) 2901
MaeI CTAG 9 cut(s) 221, 516, 552, 636, 1743, 2015, 2049, 2403, 2925
MaeII ACGT 3 cut(s) 3173, 3179, 3288
MaeIII GTNAC 8 cut(s) 1700, 1877, 2255, 2368, 2905, 3085, 3180, 3311
MfeI CAATTG 3 cut(s) 2088, 2529, 2778
MflI RGATCY 5 cut(s) 595, 1421, 2681, 3073, 3620
MhlI GDGCHC 5 cut(s) 1267, 1372, 1597, 2481, 3447
MlsI TGGCCA 1 cut(s) 251
MluI ACGCGT 1 cut(s) 2192
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 6 cut(s) 19, 190, 584, 1481, 2240, 3605
MmeI TCCRAC 4 cut(s) 409, 1634, 2335, 3066
Mox20I TGGCCA 1 cut(s) 251
MroI TCCGGA 1 cut(s) 3367
MroNI GCCGGC 1 cut(s) 1908
MroXI GAANNNNTTC 3 cut(s) 1122, 2202, 2633
MscI TGGCCA 1 cut(s) 251
MslI CAYNNNNRTG 7 cut(s) 688, 1133, 1926, 2193, 2364, 2547, 3558
Msp20I TGGCCA 1 cut(s) 251
MspI CCGG 9 cut(s) 42, 209, 1556, 1810, 1909, 2391, 2430, 3200, 3368
MspR9I CCNGG 4 cut(s) 210, 2391, 2903, 3399
MunI CAATTG 3 cut(s) 2088, 2529, 2778
Mva1269I GAATGC 4 cut(s) 904, 947, 2468, 3513
MvaI CCWGG 2 cut(s) 2903, 3399
MvnI CGCG 1 cut(s) 2194
NaeI GCCGGC 1 cut(s) 1910
NciI CCSGG 2 cut(s) 210, 2391
NcoI CCATGG 1 cut(s) 961
NgoMIV GCCGGC 1 cut(s) 1908
NheI GCTAGC 2 cut(s) 635, 2402
NmeAIII GCCGAG 1 cut(s) 926
NmuCI GTSAC 4 cut(s) 1700, 1877, 2905, 3180
NspI RCATGY 6 cut(s) 619, 1355, 1778, 1925, 3266, 3278
NspV TTCGAA 1 cut(s) 2472
PaeI GCATGC 1 cut(s) 3266
PagI TCATGA 3 cut(s) 714, 979, 3349
PciI ACATGT 1 cut(s) 1921
PctI GAATGC 4 cut(s) 904, 947, 2468, 3513
PdiI GCCGGC 1 cut(s) 1910
PdmI GAANNNNTTC 3 cut(s) 1122, 2202, 2633
PflFI GACNNNGTC 1 cut(s) 1731
PflMI CCANNNNNTGG 1 cut(s) 605
PfoI TCCNGGA 2 cut(s) 2389, 3397
PinAI ACCGGT 1 cut(s) 1555
PleI GAGTC 6 cut(s) 18, 189, 583, 1480, 2239, 3605
PpsI GAGTC 6 cut(s) 18, 189, 583, 1480, 2239, 3605
Ppu21I YACGTR 1 cut(s) 3174
PscI ACATGT 1 cut(s) 1921
PshAI GACNNNNGTC 2 cut(s) 56, 3410
PsiI TTATAA 1 cut(s) 1860
Psp124BI GAGCTC 1 cut(s) 3447
Psp6I CCWGG 2 cut(s) 2901, 3397
PspGI CCWGG 2 cut(s) 2901, 3397
PspOMI GGGCCC 1 cut(s) 2477
PspPI GGNCC 9 cut(s) 28, 229, 1283, 1849, 2122, 2477, 2478, 2806, 3343
PstI CTGCAG 2 cut(s) 1234, 3488
PsuI RGATCY 5 cut(s) 595, 1421, 2681, 3073, 3620
PsyI GACNNNGTC 1 cut(s) 1731
RsaI GTAC 5 cut(s) 34, 1428, 1601, 3117, 3420
RsaNI GTAC 5 cut(s) 33, 1427, 1600, 3116, 3419
RseI CAYNNNNRTG 7 cut(s) 688, 1133, 1926, 2193, 2364, 2547, 3558
SacI GAGCTC 1 cut(s) 3447
Sau96I GGNCC 9 cut(s) 28, 229, 1283, 1849, 2122, 2477, 2478, 2806, 3343
SchI GAGTC 6 cut(s) 19, 190, 584, 1481, 2240, 3605
ScrFI CCNGG 4 cut(s) 210, 2391, 2903, 3399
SduI GDGCHC 5 cut(s) 1267, 1372, 1597, 2481, 3447
SexAI ACCWGGT 1 cut(s) 2901
SfaNI GCATC 8 cut(s) 342, 862, 1023, 2539, 3278, 3403, 3550, 3583
SfcI CTRYAG 2 cut(s) 1230, 3484
SfuI TTCGAA 1 cut(s) 2472
SinI GGWCC 6 cut(s) 28, 229, 1283, 2122, 2806, 3343
SmiMI CAYNNNNRTG 7 cut(s) 688, 1133, 1926, 2193, 2364, 2547, 3558
SmlI CTYRAG 2 cut(s) 92, 1626
SmoI CTYRAG 2 cut(s) 92, 1626
SnaBI TACGTA 1 cut(s) 3174
SphI GCATGC 1 cut(s) 3266
SsiI CCGC 6 cut(s) 123, 336, 466, 1146, 3341, 3358
SspI AATATT 1 cut(s) 2492
SspMI CTAG 9 cut(s) 221, 516, 552, 636, 1743, 2015, 2049, 2403, 2925
SstI GAGCTC 1 cut(s) 3447
StyD4I CCNGG 4 cut(s) 208, 2389, 2901, 3397
StyI CCWWGG 4 cut(s) 873, 961, 1113, 2887
TaaI ACNGT 3 cut(s) 58, 2139, 3251
TaiI ACGT 3 cut(s) 3176, 3182, 3291
TaqI TCGA 9 cut(s) 24, 184, 383, 1356, 1372, 1475, 2472, 2773, 2826
TaqII GACCGA 1 cut(s) 58
TatI WGTACW 4 cut(s) 1426, 1599, 3115, 3418
TauI GCSGC 1 cut(s) 3360
TscAI CASTG 8 cut(s) 406, 807, 1534, 1675, 1888, 2613, 3231, 3488
TseFI GTSAC 4 cut(s) 1700, 1877, 2905, 3180
Tsp45I GTSAC 4 cut(s) 1700, 1877, 2905, 3180
TspGWI ACGGA 2 cut(s) 20, 3420
TspRI CASTG 8 cut(s) 406, 807, 1534, 1675, 1888, 2613, 3231, 3488
Tth111I GACNNNGTC 1 cut(s) 1731
Van91I CCANNNNNTGG 1 cut(s) 605
VpaK11BI GGWCC 6 cut(s) 28, 229, 1283, 2122, 2806, 3343
XapI RAATTY 5 cut(s) 547, 1802, 2002, 2071, 2199
XbaI TCTAGA 2 cut(s) 551, 2014
XceI RCATGY 6 cut(s) 619, 1355, 1778, 1925, 3266, 3278
XcmI CCANNNNNNNNNTGG 1 cut(s) 1802
XmiI GTMKAC 1 cut(s) 3176
XmnI GAANNNNTTC 3 cut(s) 1122, 2202, 2633
XspI CTAG 9 cut(s) 221, 516, 552, 636, 1743, 2015, 2049, 2403, 2925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.