Rh7BG060400

ATP-dependent helicase hrq1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0006845
Physical Location & Seq
Reverse (-)
4031241 .. 4039558
8318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG060400.1

Sequence Viewer

Length: 3630 bp
ATGGCAGCGAGTCGGATTCAAATCGAGGTCCGTACATTGACCGGTGAAGCGACCACCGTCTCAGCCTCCGTCAACGACGCCGTTTCAGACCTCAAGCTACTTCTCAACCAGTCCTTCGCTCCCGCCACCAACTCTCCCAATTTCCACCTCTTCTTCAAGGGTGAGAAGTTGAGGTTGCAGAGTCGAATAGGCACACACGGGATTAAGCCGGGTGAGGTTCTAGTGCTGGTCCCGTTTGTTAAAAAGGTGGCTAATAATCAGAGCCAGAAATGTGATCAATCTGATCCATCGTTGAATACTTCTGCTAATCGCTCCATGTCCAAGTTTGCGTACTCCGCATGGTCTGATATGATGCAGGACTTGTCGTATTTGCGTGATGGTTCGAGTGATGGAACCCCGAGTGTGTCTAATATAGGGAGTTTTAGTGTTGGAGATGGAAGTGGAGATTGCTCTCGTGAAGTGAAGCGGAAAAGGGGGTTTGGTTGTGATGAGATGATATTGGATATGTTGTGGGCTAGTAGAAGCAAGAGTGTTCTTGATGAACAGAATTCTAGAAGGTTTGTTGAGCTTTTGGGGTCGGTGAATTGTTTATCGGATCCATATTCTGGGGACTGCATGCTGTGGAGAAGAAAAGCTAGCTTACAAGGCTTTGGCTTGGGGCTGCCTAACGGCAATGGTAATTCATGCTTGTGTCCAGAGTGGTTGAAGAAAATCATGAAGGCGTTTGCTTTCTTAAACATTTTTTCTGCACTTGTTCAACTTCAGCAGGAGAGGACAACCTCAACTCTATTGGAGCAAGCACTGGAACAGCTTGCGACATTTGGAGTTAAACTTGATATGCAGGACATGGAGCATCTTTCGGTTATTTCTCCCAAGGTAGTTCTTTTTGTGAAGAAGAATGCAGAGGAGACAAGTTCTGGTAATGCACTTGTTATTATTGAATGCTCGGCAGAACAGAACCATGGAGACAGAAATAATCATGAATCTGCTTGCAAACAGATGGATGTATCAAAGATTGTCAATGAATTGAAGATGCAGGAAAGTTCTTTCAAAAGTAATTTGTGGGAGGCTGTTGAGAGGTTAATGTTTATCAAAAGAAATGAGATTTCCTCCTTGGAAGACTTTCTCATTTCTGTGAAGGATTATGGTGCTGCTGCTGCAAGAGGAAATAAAGTGAATCAAGCAACGAAAAGCCACACAGCTTCAACTACTGATTCAGACAAAAAGCACTCTGCAGCTTCAACCTCTCGTTCAGACAAAAAGCGAGCACGATGTCATAGAACAGATCCATTGCTACCAGTTGAGATGGTTGAACATCTTAGAAAGAGAATTGGATCAGCAACGAAGATAGTGCATGTCGAAGACATTGGTGCTCGAAATGCCGTTCATGTGGAAGTTCCACAGGAACTATCAGAAAATCCGAGATCTGTACTTCATTCTATTGGAATCACTCAGTTATATAGCCATCAGGCAGAGTCGATACAAGCCTCACTAACTGGCAAGAATGTTGTTGTGGCTACAATGACATCCAGTGGGAAATCTCTTTGCTACAATCTACCGGTCATTGAAGCCCTGTCCCAGAGTTCCTCATCATGTGCTCTATACTTATTTCCAACGAAGGCTTTAGCTCAAGATCAACTAAGAGCTTTCTCAGCTATGACCAAAGGATTCAGTGGTAGCTTAAATGTTGGTATATATGATGGTGACACTAATCAGCAGGAAAGGACACGACTGCGTCAAAGTGCTAGACTGCTAATCACAAATCCAGATATGTTGCATGTATCAATCCTGCCACACCACCGAAAATTTGGCCGGATTTTGGCAAATCTTAGGTATGTAGTGATTGATGAGGCCCATGTTTATAAGGGAGCATTTGGTTGTCACACTGCTCTTATACTTAGGCGACTTCGCCGGCTCTGTTCACATGTGTATGGAAGTGATCCTTCTTTTGTGTTTTCTACTGCGACTTCGGCAAATCCTCGTGAGCATTGCATGGAACTTGCGAATTTACCAACTCTAGAGTTGATTCAGAATGATGGAAGCCCTGCTGCTAGAAAGCTTTTCATCCTTTGGAATTCTGAGGACAGCGCAATTGAGGATATATCATTCCTCTTTGCTGAAATGGTCCAGCATGGACTACGGTGCATTGCATTTTGCAAAACACGCAAGCTTTGTGAACTTGTTTTTTGCTATACGCGTGAAATTCTTCAGGAGACAGCACCCCATCTGGCAGAGTCTATATGTTCTTATCGTGCTGGTTACATTGCTGAGGATAGGAGAAGAATAGAGAGTGATTTATTTAATGGGAAACTTTCTGGTATTGCTGCAACAAATGCCCTTGAATTGGGTATTGATGTTGGACACATTGATGTAACTCTTCATTTAGGTTTTCCCGGAAGTATTGCTAGCTTGTGGCAACAAGCTGGCAGAGCCGGAAGGAGAGAGAGACCTTCTCTTGCTGTATATGTTGCATCCGAAGGGCCCCTTGACCAATATTTTATGAAACATCCAAATAAACTTTTTGGAAGCCCAATTGAGTGCTGTCATGTTGATGCTAAGAACCAACAGGTTCTTGAACAGCATTTGTCTTGTGCTGCTCTTGAACACCCACTGAGCTTGTTTTATGATGAGAAGTTTTTCGGTTCTGGTTTAAACAGTGCCATAATGTCTCTTAAAAGTAGAGGACATCTGAGTTATGATCCATCATGTGATTCTTCTGCTAAAATCTGGAACTATATTGGGTATGAGAAAATGCCTTCACATTCAGTTAGTATTCGATCAATTGAAAGAGAGAGATACAAAGTTATGGACCATCAAGAGAAAGAAATCGAAGAGATTGAGGAAAGCAAGGCTTTCTTTCAGGTATATGAAGGTGCTGTTTATTTGAACCAAGGAAAGACATACCTGGTCACGAGCTTAGATTTATCTAGAAAGATTGCTTGCTGCCAAAAAGCTGATGTAAATTATTATACGAAACCCCGTGATCGCACAGATATTCATGTAGTTGGTGGTAATGTGGCTTATCCAGCTCCAGCTAAGCTTTCGAACCAGTTCTCCAGAACAACTGCCCGAGCAGATCCTTGCAGAGTAACAACTACATGGTTTGGATTCTATCGTGTACATAAAGGAAGCAACGTGATATTTGATAGAGTTAACCTTGCACTTCCTAAATATACGTATGCGTCACAGGCAGTTTGGGTTCCGGTTCCACGATCAATAATTAAAGCAGTGGAGAGTAAACAACTTGAATACCGTGCAGGGCTGCATGCTGCATCACATGCTGTTCTGCACGTTGTGCCTCTACGAATCATTTGCAACTTATCTGACTTGGCTCCAGAGTGTGCGGACCCTCATGACAGCAGATATTTTCCGGAGAGGATTCTATTATACGATCAGCATCCTGGAGGGACTGGTGTCTGTGTACAGGTTCATCCATTTTTTATGGAGCTCTTGGGTACTGCTCTGGAACTCCTAACATCTTGCCACTGCAGAGAATATAACGGTTGCCCTAATTGCATTCAGAGTTTTTGTTGCAAGTCATATAATGAGGTCTTACACAAGGATGCAGCAATTATGATCATTAAGGGTGTTCTGGATGCGAATAAATCTTCTGACTCTTCCGGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DUF1998 Helicase_C

Protein Analysis

1209

Amino Acids

134.29

Weight (kDa)

8.19

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 485 - 622 4.2e-07 Type III restriction enzyme, res subunit
DEAD PF00270 488 - 658 5.2e-31 DEAD/DEAH box helicase
Helicase_C PF00271 708 - 814 1.8e-12 Helicase conserved C-terminal domain
WHD_HRQ1 PF22982 850 - 928 3.2e-17 ATP-dependent helicase HRQ1, winged helix domain
MZB PF09369 1091 - 1173 3e-17 MrfA Zn-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1863
AccB7I CCANNNNNTGG 1 cut(s) 605
AccII CGCG 1 cut(s) 2197
AccIII TCCGGA 2 cut(s) 3370, 3620
AciI CCGC 4 cut(s) 123, 336, 466, 3344
AclWI GGATC 8 cut(s) 278, 590, 603, 1280, 1342, 1934, 2693, 3071
AcoI YGGCCR 1 cut(s) 1810
AcsI RAATTY 5 cut(s) 547, 1805, 2005, 2074, 2202
AcuI CTGAAG 2 cut(s) 746, 2192
AcyI GRCGYC 1 cut(s) 78
AdeI CACNNNGTG 1 cut(s) 3295
AfaI GTAC 6 cut(s) 34, 332, 1431, 3120, 3423, 3457
AfiI CCNNNNNNNGG 3 cut(s) 605, 1819, 2344
AflIII ACRYGT 2 cut(s) 1924, 2195
AgeI ACCGGT 2 cut(s) 41, 1558
AjnI CCWGG 2 cut(s) 2904, 3400
AloI GAACNNNNNNTCC 2 cut(s) 3038, 3070
Alw21I GWGCWC 4 cut(s) 1270, 1375, 1600, 3450
Alw26I GTCTC 6 cut(s) 64, 902, 960, 2207, 2440, 2673
AlwI GGATC 8 cut(s) 278, 590, 603, 1280, 1342, 1934, 2693, 3071
Ama87I CYCGRG 2 cut(s) 397, 3069
Aor13HI TCCGGA 2 cut(s) 3370, 3620
AoxI GGCC 3 cut(s) 1810, 1851, 2480
ApaI GGGCCC 1 cut(s) 2484
ApoI RAATTY 5 cut(s) 547, 1805, 2005, 2074, 2202
AsiGI ACCGGT 2 cut(s) 41, 1558
Asp700I GAANNNNTTC 4 cut(s) 1122, 2205, 2636, 3050
AspLEI GCGC 1 cut(s) 2090
AspS9I GGNCC 8 cut(s) 28, 229, 1852, 2125, 2480, 2481, 2809, 3346
AsuC2I CCSGG 2 cut(s) 210, 2394
AsuHPI GGTGA 5 cut(s) 56, 173, 224, 592, 1715
AsuII TTCGAA 1 cut(s) 3044
AsuNHI GCTAGC 2 cut(s) 635, 2405
AvaI CYCGRG 2 cut(s) 397, 3069
AvaII GGWCC 5 cut(s) 28, 229, 2125, 2809, 3346
BaeGI GKGCMC 1 cut(s) 2484
BamHI GGATCC 1 cut(s) 595
BanII GRGCYC 2 cut(s) 2484, 3450
BauI CACGAG 3 cut(s) 453, 1980, 2911
BbsI GAAGAC 2 cut(s) 1125, 1368
Bbv12I GWGCWC 4 cut(s) 1270, 1375, 1600, 3450
BbvCI CCTCAGC 1 cut(s) 2268
BceAI ACGGC 3 cut(s) 65, 685, 1367
BcgI CGANNNNNNTGC 4 cut(s) 1333, 1367, 3294, 3328
BciT130I CCWGG 2 cut(s) 2906, 3402
BclI TGATCA 2 cut(s) 274, 3576
BcnI CCSGG 2 cut(s) 210, 2394
BcoDI GTCTC 6 cut(s) 64, 902, 960, 2207, 2440, 2673
BfaI CTAG 9 cut(s) 221, 516, 552, 636, 1746, 2018, 2052, 2406, 2928
BfmI CTRYAG 2 cut(s) 1233, 3487
BglII AGATCT 1 cut(s) 1424
BlpI GCTNAGC 1 cut(s) 3036
Bme1390I CCNGG 4 cut(s) 210, 2394, 2906, 3402
Bme18I GGWCC 5 cut(s) 28, 229, 2125, 2809, 3346
BmeT110I CYCGRG 2 cut(s) 397, 3069
BmgT120I GGNCC 8 cut(s) 28, 229, 1852, 2125, 2480, 2481, 2809, 3346
BmiI GGNNCC 9 cut(s) 231, 394, 597, 2482, 2483, 3201, 3207, 3333, 3348
BmrFI CCNGG 4 cut(s) 210, 2394, 2906, 3402
BmsI GCATC 9 cut(s) 342, 862, 1023, 2480, 2542, 3281, 3406, 3553, 3586
BmtI GCTAGC 2 cut(s) 639, 2409
BoxI GACNNNNGTC 2 cut(s) 56, 3413
BpiI GAAGAC 2 cut(s) 1125, 1368
BplI GAGNNNNNCTC 4 cut(s) 1094, 1126, 2437, 2469
BpmI CTGGAG 4 cut(s) 3015, 3040, 3318, 3423
Bpu10I CCTNAGC 1 cut(s) 2268
Bpu1102I GCTNAGC 1 cut(s) 3036
Bpu14I TTCGAA 1 cut(s) 3044
BpuEI CTTGAG 2 cut(s) 77, 1614
BpuMI CCSGG 2 cut(s) 210, 2394
BsaAI YACGTR 1 cut(s) 3177
BsaBI GATNNNNATC 2 cut(s) 20, 3396
BsaHI GRCGYC 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 2440
BsaJI CCNNGG 4 cut(s) 873, 961, 1113, 2890
BsaWI WCCGGW 5 cut(s) 41, 1558, 3202, 3370, 3620
BsaXI ACNNNNNCTCC 6 cut(s) 118, 148, 409, 439, 3038, 3068
Bsc4I CCNNNNNNNGG 3 cut(s) 605, 1819, 2344
Bse118I RCCGGY 3 cut(s) 41, 1558, 1911
Bse1I ACTGG 7 cut(s) 109, 807, 1298, 1501, 1530, 3049, 3415
Bse3DI GCAATG 5 cut(s) 679, 1289, 1987, 2145, 2262
Bse8I GATNNNNATC 2 cut(s) 20, 3396
BseAI TCCGGA 2 cut(s) 3370, 3620
BseBI CCWGG 2 cut(s) 2906, 3402
BseDI CCNNGG 4 cut(s) 873, 961, 1113, 2890
BseGI GGATG 9 cut(s) 1009, 1526, 2064, 2471, 2506, 3397, 3430, 3568, 3601
BseJI GATNNNNATC 2 cut(s) 20, 3396
BseLI CCNNNNNNNGG 3 cut(s) 605, 1819, 2344
BseMI GCAATG 5 cut(s) 679, 1289, 1987, 2145, 2262
BseMII CTCAG 7 cut(s) 75, 1466, 1665, 2070, 2259, 2603, 2681
BseNI ACTGG 7 cut(s) 109, 807, 1298, 1501, 1530, 3049, 3415
BseRI GAGGAG 1 cut(s) 920
BseSI GKGCMC 1 cut(s) 2484
BsgI GTGCAG 3 cut(s) 732, 3272, 3276
Bsh1236I CGCG 1 cut(s) 2197
BshFI GGCC 3 cut(s) 1812, 1853, 2482
BshTI ACCGGT 2 cut(s) 41, 1558
BsiHKAI GWGCWC 4 cut(s) 1270, 1375, 1600, 3450
BsiHKCI CYCGRG 2 cut(s) 397, 3069
BslFI GGGAC 4 cut(s) 215, 623, 1561, 3421
BslI CCNNNNNNNGG 3 cut(s) 605, 1819, 2344
BsmAI GTCTC 6 cut(s) 64, 902, 960, 2207, 2440, 2673
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 4 cut(s) 215, 623, 1561, 3421
BsmI GAATGC 3 cut(s) 904, 947, 3516
BsnI GGCC 3 cut(s) 1812, 1853, 2482
Bso31I GGTCTC 1 cut(s) 2440
BsoBI CYCGRG 2 cut(s) 397, 3069
Bsp119I TTCGAA 1 cut(s) 3044
Bsp120I GGGCCC 1 cut(s) 2480
Bsp1286I GDGCHC 5 cut(s) 1270, 1375, 1600, 2484, 3450
Bsp13I TCCGGA 2 cut(s) 3370, 3620
Bsp1407I TGTACA 2 cut(s) 3118, 3421
Bsp1720I GCTNAGC 1 cut(s) 3036
Bsp19I CCATGG 1 cut(s) 961
BspACI CCGC 4 cut(s) 123, 336, 466, 3344
BspANI GGCC 3 cut(s) 1812, 1853, 2482
BspCNI CTCAG 7 cut(s) 74, 1465, 1664, 2071, 2260, 2604, 2682
BspEI TCCGGA 2 cut(s) 3370, 3620
BspFNI CGCG 1 cut(s) 2197
BspHI TCATGA 3 cut(s) 714, 979, 3352
BspLI GGNNCC 9 cut(s) 231, 394, 597, 2482, 2483, 3201, 3207, 3333, 3348
BspMAI CTGCAG 2 cut(s) 1237, 3491
BspOI GCTAGC 2 cut(s) 639, 2409
BspPI GGATC 8 cut(s) 278, 590, 603, 1280, 1342, 1934, 2693, 3071
BspT104I TTCGAA 1 cut(s) 3044
BspTNI GGTCTC 1 cut(s) 2440
BsrDI GCAATG 5 cut(s) 679, 1289, 1987, 2145, 2262
BsrFI RCCGGY 3 cut(s) 41, 1558, 1911
BsrGI TGTACA 2 cut(s) 3118, 3421
BsrI ACTGG 7 cut(s) 109, 807, 1298, 1501, 1530, 3049, 3415
BssAI RCCGGY 3 cut(s) 41, 1558, 1911
BssECI CCNNGG 4 cut(s) 873, 961, 1113, 2890
BssNI GRCGYC 1 cut(s) 78
BssSI CACGAG 3 cut(s) 453, 1980, 2911
BssT1I CCWWGG 4 cut(s) 873, 961, 1113, 2890
Bst2BI CACGAG 3 cut(s) 453, 1980, 2911
Bst2UI CCWGG 2 cut(s) 2906, 3402
Bst4CI ACNGT 5 cut(s) 58, 2142, 2657, 3254, 3503
Bst6I CTCTTC 4 cut(s) 155, 2382, 2826, 3622
BstACI GRCGYC 1 cut(s) 78
BstAPI GCANNNNNTGC 3 cut(s) 2333, 3278, 3295
BstAUI TGTACA 2 cut(s) 3118, 3421
BstBAI YACGTR 1 cut(s) 3177
BstBI TTCGAA 1 cut(s) 3044
BstDSI CCRYGG 1 cut(s) 961
BstF5I GGATG 9 cut(s) 1009, 1526, 2064, 2471, 2506, 3397, 3430, 3568, 3601
BstFNI CGCG 1 cut(s) 2197
BstHHI GCGC 1 cut(s) 2090
BstMAI GTCTC 6 cut(s) 64, 902, 960, 2207, 2440, 2673
BstNI CCWGG 2 cut(s) 2906, 3402
BstNSI RCATGY 6 cut(s) 619, 1358, 1781, 1928, 3269, 3281
BstPAI GACNNNNGTC 2 cut(s) 56, 3413
BstSCI CCNGG 4 cut(s) 208, 2392, 2904, 3400
BstSFI CTRYAG 2 cut(s) 1233, 3487
BstSLI GKGCMC 1 cut(s) 2484
BstSNI TACGTA 1 cut(s) 3177
BstUI CGCG 1 cut(s) 2197
BstV2I GAAGAC 2 cut(s) 1125, 1368
BstX2I RGATCY 5 cut(s) 595, 1285, 1424, 3076, 3623
BstYI RGATCY 5 cut(s) 595, 1285, 1424, 3076, 3623
BsuRI GGCC 3 cut(s) 1812, 1853, 2482
BtgI CCRYGG 1 cut(s) 961
BtsCI GGATG 9 cut(s) 1009, 1526, 2064, 2471, 2506, 3397, 3430, 3568, 3601
BtsI GCAGTG 3 cut(s) 1884, 3234, 3484
BtsIMutI CAGTG 8 cut(s) 800, 1537, 1678, 1884, 2609, 2662, 3234, 3484
CciI TCATGA 3 cut(s) 714, 979, 3352
CfoI GCGC 1 cut(s) 2090
Cfr10I RCCGGY 3 cut(s) 41, 1558, 1911
Cfr13I GGNCC 8 cut(s) 28, 229, 1852, 2125, 2480, 2481, 2809, 3346
CseI GACGC 3 cut(s) 86, 1724, 3171
CsiI ACCWGGT 1 cut(s) 2904
Csp6I GTAC 6 cut(s) 33, 331, 1430, 3119, 3422, 3456
CspAI ACCGGT 2 cut(s) 41, 1558
CspCI CAANNNNNGTGG 2 cut(s) 2598, 2633
CviQI GTAC 6 cut(s) 33, 331, 1430, 3119, 3422, 3456
DraI TTTAAA 1 cut(s) 2652
DraIII CACNNNGTG 1 cut(s) 3295
EaeI YGGCCR 1 cut(s) 1810
Eam1104I CTCTTC 4 cut(s) 155, 2382, 2826, 3622
EarI CTCTTC 4 cut(s) 155, 2382, 2826, 3622
Ecl136II GAGCTC 1 cut(s) 3448
Eco105I TACGTA 1 cut(s) 3177
Eco130I CCWWGG 4 cut(s) 873, 961, 1113, 2890
Eco24I GRGCYC 2 cut(s) 2484, 3450
Eco31I GGTCTC 1 cut(s) 2440
Eco47I GGWCC 5 cut(s) 28, 229, 2125, 2809, 3346
Eco53kI GAGCTC 1 cut(s) 3448
Eco57I CTGAAG 2 cut(s) 746, 2192
Eco88I CYCGRG 2 cut(s) 397, 3069
EcoICRI GAGCTC 1 cut(s) 3448
EcoO109I RGGNCCY 2 cut(s) 2480, 2481
EcoRI GAATTC 2 cut(s) 547, 2074
EcoRII CCWGG 2 cut(s) 2904, 3400
EcoT14I CCWWGG 4 cut(s) 873, 961, 1113, 2890
EcoT38I GRGCYC 2 cut(s) 2484, 3450
ErhI CCWWGG 4 cut(s) 873, 961, 1113, 2890
Esp3I CGTCTC 1 cut(s) 64
FalI AAGNNNNNCTT 8 cut(s) 1927, 1959, 2469, 2501, 2836, 2868, 2840, 2872
FaqI GGGAC 4 cut(s) 215, 623, 1561, 3421
FauI CCCGC 1 cut(s) 130
FbaI TGATCA 2 cut(s) 274, 3576
FokI GGATG 9 cut(s) 1016, 1513, 2051, 2458, 2493, 3384, 3417, 3575, 3608
FriOI GRGCYC 2 cut(s) 2484, 3450
FspBI CTAG 9 cut(s) 221, 516, 552, 636, 1746, 2018, 2052, 2406, 2928
GlaI GCGC 1 cut(s) 2089
GsuI CTGGAG 4 cut(s) 3015, 3040, 3318, 3423
HaeIII GGCC 3 cut(s) 1812, 1853, 2482
HgaI GACGC 3 cut(s) 86, 1724, 3171
HhaI GCGC 1 cut(s) 2090
Hin1I GRCGYC 1 cut(s) 78
Hin6I GCGC 1 cut(s) 2088
HinP1I GCGC 1 cut(s) 2088
HincII GTYRAC 2 cut(s) 73, 3154
HindII GTYRAC 2 cut(s) 73, 3154
HindIII AAGCTT 3 cut(s) 2057, 2168, 3038
HpaI GTTAAC 1 cut(s) 3154
HphI GGTGA 5 cut(s) 56, 173, 224, 592, 1715
Hpy166II GTNNAC 7 cut(s) 73, 1922, 2177, 3119, 3154, 3239, 3422
Hpy8I GTNNAC 7 cut(s) 73, 1922, 2177, 3119, 3154, 3239, 3422
Hpy99I CGWCG 1 cut(s) 80
HpyCH4III ACNGT 5 cut(s) 58, 2142, 2657, 3254, 3503
HpyCH4IV ACGT 3 cut(s) 3135, 3176, 3291
HpySE526I ACGT 3 cut(s) 3135, 3176, 3291
Hsp92I GRCGYC 1 cut(s) 78
HspAI GCGC 1 cut(s) 2088
Kpn2I TCCGGA 2 cut(s) 3370, 3620
KroI GCCGGC 1 cut(s) 1911
KroNI GCCGGC 1 cut(s) 1913
Ksp22I TGATCA 2 cut(s) 274, 3576
KspAI GTTAAC 1 cut(s) 3154
LmnI GCTCC 8 cut(s) 124, 317, 793, 850, 1868, 3034, 3337, 3445
LweI GCATC 9 cut(s) 342, 862, 1023, 2480, 2542, 3281, 3406, 3553, 3586
MabI ACCWGGT 1 cut(s) 2904
MaeI CTAG 9 cut(s) 221, 516, 552, 636, 1746, 2018, 2052, 2406, 2928
MaeII ACGT 3 cut(s) 3135, 3176, 3291
MaeIII GTNAC 7 cut(s) 1703, 1880, 2258, 2371, 2908, 3088, 3183
MfeI CAATTG 3 cut(s) 2091, 2532, 2781
MflI RGATCY 5 cut(s) 595, 1285, 1424, 3076, 3623
MhlI GDGCHC 5 cut(s) 1270, 1375, 1600, 2484, 3450
MluI ACGCGT 1 cut(s) 2195
MlyI GAGTC 5 cut(s) 19, 190, 1484, 2243, 3608
MmeI TCCRAC 3 cut(s) 409, 1637, 2338
MroI TCCGGA 2 cut(s) 3370, 3620
MroNI GCCGGC 1 cut(s) 1911
MroXI GAANNNNTTC 4 cut(s) 1122, 2205, 2636, 3050
MslI CAYNNNNRTG 6 cut(s) 688, 1133, 1929, 2367, 2550, 3561
MspR9I CCNGG 4 cut(s) 210, 2394, 2906, 3402
MssI GTTTAAAC 1 cut(s) 2652
MunI CAATTG 3 cut(s) 2091, 2532, 2781
Mva1269I GAATGC 3 cut(s) 904, 947, 3516
MvaI CCWGG 2 cut(s) 2906, 3402
MvnI CGCG 1 cut(s) 2197
NaeI GCCGGC 1 cut(s) 1913
NciI CCSGG 2 cut(s) 210, 2394
NcoI CCATGG 1 cut(s) 961
NgoMIV GCCGGC 1 cut(s) 1911
NheI GCTAGC 2 cut(s) 635, 2405
NlaIV GGNNCC 9 cut(s) 231, 394, 597, 2482, 2483, 3201, 3207, 3333, 3348
NmeAIII GCCGAG 1 cut(s) 926
NmuCI GTSAC 4 cut(s) 1703, 1880, 2908, 3183
NspI RCATGY 6 cut(s) 619, 1358, 1781, 1928, 3269, 3281
NspV TTCGAA 1 cut(s) 3044
PaeI GCATGC 2 cut(s) 619, 3269
PagI TCATGA 3 cut(s) 714, 979, 3352
PciI ACATGT 1 cut(s) 1924
PctI GAATGC 3 cut(s) 904, 947, 3516
PdiI GCCGGC 1 cut(s) 1913
PdmI GAANNNNTTC 4 cut(s) 1122, 2205, 2636, 3050
PflFI GACNNNGTC 1 cut(s) 1734
PflMI CCANNNNNTGG 1 cut(s) 605
PfoI TCCNGGA 2 cut(s) 2392, 3400
PinAI ACCGGT 2 cut(s) 41, 1558
PleI GAGTC 5 cut(s) 18, 189, 1483, 2242, 3608
PmeI GTTTAAAC 1 cut(s) 2652
PpsI GAGTC 5 cut(s) 18, 189, 1483, 2242, 3608
Ppu21I YACGTR 1 cut(s) 3177
PscI ACATGT 1 cut(s) 1924
PshAI GACNNNNGTC 2 cut(s) 56, 3413
PsiI TTATAA 1 cut(s) 1863
Psp124BI GAGCTC 1 cut(s) 3450
Psp6I CCWGG 2 cut(s) 2904, 3400
PspGI CCWGG 2 cut(s) 2904, 3400
PspN4I GGNNCC 9 cut(s) 231, 394, 597, 2482, 2483, 3201, 3207, 3333, 3348
PspOMI GGGCCC 1 cut(s) 2480
PspPI GGNCC 8 cut(s) 28, 229, 1852, 2125, 2480, 2481, 2809, 3346
PstI CTGCAG 2 cut(s) 1237, 3491
PsuI RGATCY 5 cut(s) 595, 1285, 1424, 3076, 3623
PsyI GACNNNGTC 1 cut(s) 1734
RsaI GTAC 6 cut(s) 34, 332, 1431, 3120, 3423, 3457
RsaNI GTAC 6 cut(s) 33, 331, 1430, 3119, 3422, 3456
RseI CAYNNNNRTG 6 cut(s) 688, 1133, 1929, 2367, 2550, 3561
SacI GAGCTC 1 cut(s) 3450
Sau96I GGNCC 8 cut(s) 28, 229, 1852, 2125, 2480, 2481, 2809, 3346
SchI GAGTC 5 cut(s) 19, 190, 1484, 2243, 3608
ScrFI CCNGG 4 cut(s) 210, 2394, 2906, 3402
SduI GDGCHC 5 cut(s) 1270, 1375, 1600, 2484, 3450
SexAI ACCWGGT 1 cut(s) 2904
SfaNI GCATC 9 cut(s) 342, 862, 1023, 2480, 2542, 3281, 3406, 3553, 3586
SfcI CTRYAG 2 cut(s) 1233, 3487
SfuI TTCGAA 1 cut(s) 3044
SinI GGWCC 5 cut(s) 28, 229, 2125, 2809, 3346
SmiMI CAYNNNNRTG 6 cut(s) 688, 1133, 1929, 2367, 2550, 3561
SmlI CTYRAG 2 cut(s) 92, 1629
SmoI CTYRAG 2 cut(s) 92, 1629
SnaBI TACGTA 1 cut(s) 3177
SphI GCATGC 2 cut(s) 619, 3269
SsiI CCGC 4 cut(s) 123, 336, 466, 3344
SspI AATATT 1 cut(s) 2495
SspMI CTAG 9 cut(s) 221, 516, 552, 636, 1746, 2018, 2052, 2406, 2928
SstI GAGCTC 1 cut(s) 3450
StyD4I CCNGG 4 cut(s) 208, 2392, 2904, 3400
StyI CCWWGG 4 cut(s) 873, 961, 1113, 2890
TaaI ACNGT 5 cut(s) 58, 2142, 2657, 3254, 3503
TaiI ACGT 3 cut(s) 3138, 3179, 3294
TaqI TCGA 9 cut(s) 24, 184, 383, 1359, 1375, 1478, 2776, 2829, 3044
TatI WGTACW 3 cut(s) 1429, 3118, 3421
TscAI CASTG 8 cut(s) 807, 1537, 1678, 1891, 2616, 2662, 3234, 3491
TseFI GTSAC 4 cut(s) 1703, 1880, 2908, 3183
Tsp45I GTSAC 4 cut(s) 1703, 1880, 2908, 3183
TspGWI ACGGA 2 cut(s) 20, 58
TspRI CASTG 8 cut(s) 807, 1537, 1678, 1891, 2616, 2662, 3234, 3491
Tth111I GACNNNGTC 1 cut(s) 1734
Van91I CCANNNNNTGG 1 cut(s) 605
VpaK11BI GGWCC 5 cut(s) 28, 229, 2125, 2809, 3346
XapI RAATTY 5 cut(s) 547, 1805, 2005, 2074, 2202
XbaI TCTAGA 3 cut(s) 551, 2017, 2927
XceI RCATGY 6 cut(s) 619, 1358, 1781, 1928, 3269, 3281
XcmI CCANNNNNNNNNTGG 1 cut(s) 1805
XmnI GAANNNNTTC 4 cut(s) 1122, 2205, 2636, 3050
XspI CTAG 9 cut(s) 221, 516, 552, 636, 1746, 2018, 2052, 2406, 2928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.