Rroxscaffold_3G00270310

ATP-dependent helicase hrq1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006845
Physical Location & Seq
Forward (+)
62901239 .. 62909686
8448 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00270310.1

Sequence Viewer

Length: 3903 bp
ATGGCAGCGAGTCGGATTCAAATCGAGGTCCGTACATTGACCGGCGAAGCGACCACCGTCTCAGCCTCCGTCAACGACGCCGTTTCAGACCTCAAGCTACTTCTCAACCAGTCCTTCCCTCCCGCCACCAACTCTCCCAATTTCCACCTCTTCTTCAAGGGTGAGAAGTTGAGGTTGCAGAGTCGAATAGGCACACACGGGATTAAGCCGGGTGAGGTTCTAGTGCTGGTCCCGTTTGTTAAAAAGGTGGCTAATAATCAGAGCCAGAAATTTGGTCAATCTGATCCATCGTTGAATACTTCTGCTAATTGCTCCATGTCCAAGTTTGCGAACTCCGCATGGTCTGATATGATGCAGGACCTGTCGTATTTGCGTGATGGTTCGAGTGATGGAACCCCGAGTGTGTCTAATATAGGGAGTTTTAGTGTTGGAGATGGAAGTGGAGATTGCTCTCGTGAAGTGAAGCGGAAAAGGGGGTTTGGTTGTGATGAGATGATATTGGATATGTTGTGGGCTAGTAGAAGCAAGAGTGTTCTTGATGAACAGAATTCTAGAAGGTTTGTTGAGCTTTTGGAGTCGGTGAATTGTTTATCGGATCCATATTCTGGGGACTGCATGTTGTGGAGAAGAAAAGCAAGCTTACAAGGCTTTGGCTTAGGGCTGCCTAGCGGCAATGGTAATTCATGCTTGTGTCCAGAGTGGTTGAAGAAAATCATGAAGGCGTTTGCTTTCTTAAACAGTTTTTCTGCACTTGTTCAACTTCAGCAGGAGAGGACAACCTCAACTCTGTTGGAGCAAGCACTGGAACAGCTTGCGACATTTGGAGTTAAACTTGATATGCAGGACATGGAGCATCTTTCGGTTATTTCTCCCAAGGTAGTTCTTTTTGTGAAGAAGAATGCAGAGGAGACAAGTTCTGGTAATGCACTTGTTATTATTGAATTCTCGGTAGAACAGAACCATGGAGACAGAAATAATCATGAATCTGCTTGCAAACAGATGGATGTATCAAAGATTGTCAATGAATTGAAGATGCAGGAAAGTTCTTTCAAAAGTAATTTGTGGGAGGCTGTTGAGAGGTTAATGTTTATCAAAAGAAATGAGATTTCCTCCTTGGAAGACTTTCTCATTTCTGTGAAGGATTGTGGTGCTGGTGCAAGAGGAAATAAAGTGAATCAAGCAACGAAAAGCCACACAGCTTCAACTACTGATTCAGACAAAAAGCACTCTGCAACTTCAACCTCTCGTTCAGACAAAAAGCGAGCACGATGTCATGCATCTTGTAATCCTTACTCTGGGAGATCAAAAGACTTTAATTGCAATATTTTTTCTGAGCAGAGAACAGATCCATTGCTACCATTTGAGATGGTTGAACATCTTAGAAAGAGAATTGGATCAGCAACGAAGATAGTGCATGTCGAAGACATTGGTGCTCGAAATGCCGTTCATGTGGAAGTTCCACAGGAACTATCAGAAAATCTGAGATCTGTACTTCATTCTATTGGAATCACTCAGTTATATAGCCATCAGGCAGAGTCGATACAAGCCTCACTAACTGGCAAGAATGTTGTTGTGGCCACAATGACATCCAGTGGGAAATCTCTTTGCTACAATCTACCGGTCATTGAAGCCCTGTCCCAGAGTTCCTCATCATGTGCTCTATACTTATTTCCAACGAAGGCTTTAGCTCAAGATCAACTAAGAGCTTTCTTAGCTATGACCAAAGGAGTCAGTGGTAGCTTAAATGTTGGTATATATGATGGTGACACTAATCAGCAGGAAAGGACACAACTGCGTCAAAATGCTAGATTGCTAATCACAAATCCAGATATGTTGCATGTATCAATCCTGCCACACCACCGAAAATTTGGCCGGATTTTGGCAAATCTTAGGTATGTAGTGATTGATGAGGCCCATGTTTATAAGGGAGCATTTGGTTGTCACACTGCTCTTATACTTAGGCGACTTCGCCGGCTCTGTTCACATGTGTATGGAAGTGATCCTTCTTTTGTGTTTTCTACTGCAACTTCGGCAAATCCTCGTGAACATTGCATGGAACTTGCGAATTTACCAACTCTAGAGTTGATTCAGAATGATGGAAGCCCTGCTGCTAGAAAGCTTTTCATCCTTTGGAATTCTGAGGACAGGTTGACAAAGATAATTGCTTATACGTTATTTGAGAAATGGTTTTCCGCATCTCTGGAGATCAAACATGAGGTGTGGTCTTGGAGCTTGAATACTGATGCTTTAAGTAATCTTGACAGCGCAATTGAGGATATATCATTCCTCTTTGCTGAAATGGTCCAGCATGGACTACGGTGCATTGCATTTTGCAAAACACGCAAGCTTTGTGAACTTGTTTTTTGCCATACGCGTGAAATTCTTCAGGAGACAGCACCCCATCTGGCAGAGTCTATATGCTCTTATCGTGCTGGTTACATTGCTGAGGATAGGAGAAGAATAGAGAGTGATTTATTTAACGGGAAACTTTCTGGTATTGCTGCAACAAATGCCCTTGAATTGGGTATTGATGTCGGACACATTGATGTAACTCTTCATTTGGGTTTTCCCGGAAGTATTGCTAGCTTGTGGCAACAAGCTGGCAGAGCCGGAAGGAGAGAGAGACCTTCTCTTGCTGTATATGTTGCATTCGAAGGGCCCCTTGACCAATATTTTATGAAACATCCAAATAAACTTTTTGGAAGCCCAATTGAGTGCTGTCATGTTGATGCTAAGAACCAACAGGTTCTTGAACAGCATTTGGCTTGTGCTGCTCTTGAACACCCACTGAGCTTGTTTTATGATGAGAAGTTTTTTGGTTCTGGTTTAAACAGCGCCATAATGTCTCTTAAAAGTAGAGGATATCTGAGTTATGATCCATCATGTGATTCTTCTGCTAAAATCTGGAACTATATTGGGTATCAGAAAATGCATTCACATTCAGTTAGTATTCGTTCAATTGAAAGAGTGAGATACAAAGTTATGGACCATCAAGAGAAAGAAATCGAAGAGATTGAGGAAAGCAAGGCTTTCTTTCAGGTATATGAAGGTGCTGTTTATTTGAACCAAGGAAAGACATACCTGGTCACGAGCTTAGATTTATCTAGCAAGATTGCTTGGTGCCAAAAAGCTGATGTAAATTATTATACGAAACCCCGTGATCGCACAGATATTCATGTAGTTGGTGGTAATGTGGCTTATCCAGCTCCAGCTAAGCTTTCGAACCAGTTCTCCAGAACAACTGCCCGAGCAGATCCTTGCAGAGTAACAACTACATGGTTTGGATTCTATCGTGTACTGAAAGGAAGCAACATGATATTAGATAAAGTTAACCTTGCACTTCCTAAATATACGTATACGTCACAGGCAGTTTGGGTTCCGGTTCCACTATCAACAATTAAAGCAGTGGAGAGTAAACAACTTGAATACCGTGCAGGGCTGCATGCTGCATCACATGCTGTTCTGCACGTTGTGCCTCTACGAATCATTTGTAACTTATCTGACTTGGCTCCAGAGTGTGCGGACCCTCATGACAGCCGCTATTTTCCAGAGAGGATTCTATTATATGATCAGCATCCTGGAGGGACTGGTGTCTGTGTACAGGTAAATGTGATGATACTAAATAATGGCAAGGATACCATATATGATCAGTTCACAGGTTATGCTGGACTGTTCAGCAATCACCATGGACGCTGTGATGTCCATCCATTTTTTATGGAGCTCTTGGCTACTGCTCTGGAACTCCTAACATCTTGCCACTGCAGAGAATATGATGGTTGCCCTAATTGCATTCAGAGTTTTTGTTGCAAGTCATATAATGAGGTTTTACACAAGGATGCAGCAATTATGATCATTAAGGGTGTTCTGGATGCGAATAAATCTTCTGACTCTTCTGGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DUF1998 Helicase_C

Protein Analysis

1300

Amino Acids

144.76

Weight (kDa)

7.66

Isoelectric Point (pI)

44.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 505 - 642 2.9e-07 Type III restriction enzyme, res subunit
DEAD PF00270 508 - 678 9.6e-31 DEAD/DEAH box helicase
Helicase_C PF00271 767 - 873 1.7e-12 Helicase conserved C-terminal domain
WHD_HRQ1 PF22982 909 - 987 6.4e-15 ATP-dependent helicase HRQ1, winged helix domain
MZB PF09369 1150 - 1264 6.2e-14 MrfA Zn-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1923
AccB1I GGYRCC 1 cut(s) 3120
AccB7I CCANNNNNTGG 1 cut(s) 605
AccI GTMKAC 1 cut(s) 3356
AccII CGCG 1 cut(s) 2374
AciI CCGC 7 cut(s) 123, 336, 466, 669, 2193, 3521, 3538
AclWI GGATC 8 cut(s) 278, 590, 603, 1340, 1402, 1994, 2870, 3248
AcoI YGGCCR 2 cut(s) 1575, 1870
AcsI RAATTY 7 cut(s) 269, 547, 941, 1865, 2065, 2134, 2379
AcuI CTGAAG 2 cut(s) 746, 2369
AcyI GRCGYC 1 cut(s) 78
AdeI CACNNNGTG 1 cut(s) 3472
AfaI GTAC 4 cut(s) 34, 1491, 3297, 3600
AfiI CCNNNNNNNGG 4 cut(s) 605, 1295, 1879, 2521
AflIII ACRYGT 2 cut(s) 1984, 2372
AgeI ACCGGT 1 cut(s) 1618
AjnI CCWGG 2 cut(s) 3081, 3577
AloI GAACNNNNNNTCC 2 cut(s) 3215, 3247
Alw21I GWGCWC 4 cut(s) 1267, 1435, 1660, 3723
Alw26I GTCTC 6 cut(s) 64, 902, 960, 2384, 2617, 2850
AlwI GGATC 8 cut(s) 278, 590, 603, 1340, 1402, 1994, 2870, 3248
AlwNI CAGNNNCTG 1 cut(s) 361
Ama87I CYCGRG 2 cut(s) 397, 3246
AoxI GGCC 4 cut(s) 1575, 1870, 1911, 2657
ApaI GGGCCC 1 cut(s) 2661
ApeKI GCWGC 8 cut(s) 5, 661, 2108, 2501, 2771, 3439, 3446, 3839
ApoI RAATTY 7 cut(s) 269, 547, 941, 1865, 2065, 2134, 2379
AsiGI ACCGGT 1 cut(s) 1618
Asp700I GAANNNNTTC 3 cut(s) 1122, 2382, 3227
AspLEI GCGC 2 cut(s) 2267, 2837
AspS9I GGNCC 9 cut(s) 28, 229, 358, 1912, 2302, 2657, 2658, 2986, 3523
AsuC2I CCSGG 2 cut(s) 210, 2571
AsuHPI GGTGA 5 cut(s) 173, 224, 592, 1775, 3674
AsuII TTCGAA 2 cut(s) 2652, 3221
AsuNHI GCTAGC 1 cut(s) 2582
AvaI CYCGRG 2 cut(s) 397, 3246
AvaII GGWCC 6 cut(s) 28, 229, 358, 2302, 2986, 3523
BaeGI GKGCMC 1 cut(s) 2661
BalI TGGCCA 1 cut(s) 1577
BamHI GGATCC 1 cut(s) 595
BanI GGYRCC 1 cut(s) 3120
BanII GRGCYC 2 cut(s) 2661, 3723
BauI CACGAG 3 cut(s) 453, 2040, 3088
BbsI GAAGAC 2 cut(s) 1125, 1428
Bbv12I GWGCWC 4 cut(s) 1267, 1435, 1660, 3723
BbvCI CCTCAGC 1 cut(s) 2445
BbvI GCAGC 8 cut(s) 17, 648, 2095, 2488, 2758, 3426, 3433, 3851
BceAI ACGGC 2 cut(s) 65, 1427
BcgI CGANNNNNNTGC 2 cut(s) 1393, 1427
BciT130I CCWGG 2 cut(s) 3083, 3579
BciVI GTATCC 1 cut(s) 3628
BclI TGATCA 3 cut(s) 3568, 3646, 3849
BcnI CCSGG 2 cut(s) 210, 2571
BcoDI GTCTC 6 cut(s) 64, 902, 960, 2384, 2617, 2850
BfaI CTAG 9 cut(s) 221, 516, 552, 666, 1806, 2078, 2112, 2583, 3105
BfmI CTRYAG 1 cut(s) 3760
BfoI RGCGCY 1 cut(s) 2838
BfuI GTATCC 1 cut(s) 3628
BglII AGATCT 1 cut(s) 1484
BlpI GCTNAGC 1 cut(s) 3213
Bme1390I CCNGG 4 cut(s) 210, 2571, 3083, 3579
Bme18I GGWCC 6 cut(s) 28, 229, 358, 2302, 2986, 3523
BmeT110I CYCGRG 2 cut(s) 397, 3246
BmgT120I GGNCC 9 cut(s) 28, 229, 358, 1912, 2302, 2657, 2658, 2986, 3523
BmrFI CCNGG 4 cut(s) 210, 2571, 3083, 3579
BmtI GCTAGC 1 cut(s) 2586
BoxI GACNNNNGTC 2 cut(s) 56, 3590
BpiI GAAGAC 2 cut(s) 1125, 1428
BplI GAGNNNNNCTC 4 cut(s) 1094, 1126, 2614, 2646
BpmI CTGGAG 5 cut(s) 2222, 3192, 3217, 3495, 3600
Bpu10I CCTNAGC 2 cut(s) 655, 2445
Bpu1102I GCTNAGC 1 cut(s) 3213
Bpu14I TTCGAA 2 cut(s) 2652, 3221
BpuEI CTTGAG 2 cut(s) 77, 1674
BpuMI CCSGG 2 cut(s) 210, 2571
BsaAI YACGTR 1 cut(s) 3354
BsaBI GATNNNNATC 3 cut(s) 20, 3573, 3702
BsaHI GRCGYC 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 2617
BsaJI CCNNGG 5 cut(s) 873, 961, 1113, 3067, 3685
BsaWI WCCGGW 2 cut(s) 1618, 3379
BsaXI ACNNNNNCTCC 6 cut(s) 118, 148, 409, 439, 3215, 3245
Bsc4I CCNNNNNNNGG 4 cut(s) 605, 1295, 1879, 2521
Bse118I RCCGGY 3 cut(s) 41, 1618, 1971
Bse1I ACTGG 6 cut(s) 109, 807, 1561, 1590, 3226, 3592
Bse3DI GCAATG 5 cut(s) 679, 1349, 2047, 2322, 2439
Bse8I GATNNNNATC 3 cut(s) 20, 3573, 3702
BseBI CCWGG 2 cut(s) 3083, 3579
BseDI CCNNGG 5 cut(s) 873, 961, 1113, 3067, 3685
BseGI GGATG 8 cut(s) 1009, 1586, 2124, 2683, 3574, 3703, 3841, 3874
BseJI GATNNNNATC 3 cut(s) 20, 3573, 3702
BseLI CCNNNNNNNGG 4 cut(s) 605, 1295, 1879, 2521
BseMI GCAATG 5 cut(s) 679, 1349, 2047, 2322, 2439
BseMII CTCAG 8 cut(s) 75, 1323, 1472, 1526, 2130, 2436, 2780, 2858
BseNI ACTGG 6 cut(s) 109, 807, 1561, 1590, 3226, 3592
BseRI GAGGAG 1 cut(s) 920
BseSI GKGCMC 1 cut(s) 2661
BseXI GCAGC 8 cut(s) 17, 648, 2095, 2488, 2758, 3426, 3433, 3851
BsgI GTGCAG 3 cut(s) 732, 3449, 3453
Bsh1236I CGCG 1 cut(s) 2374
BshFI GGCC 4 cut(s) 1577, 1872, 1913, 2659
BshNI GGYRCC 1 cut(s) 3120
BshTI ACCGGT 1 cut(s) 1618
BsiHKAI GWGCWC 4 cut(s) 1267, 1435, 1660, 3723
BsiHKCI CYCGRG 2 cut(s) 397, 3246
BsiSI CCGG 8 cut(s) 42, 209, 1619, 1873, 1972, 2571, 2610, 3380
BslFI GGGAC 4 cut(s) 215, 623, 1621, 3598
BslI CCNNNNNNNGG 4 cut(s) 605, 1295, 1879, 2521
BsmAI GTCTC 6 cut(s) 64, 902, 960, 2384, 2617, 2850
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 4 cut(s) 215, 623, 1621, 3598
BsmI GAATGC 4 cut(s) 904, 2648, 2932, 3789
BsnI GGCC 4 cut(s) 1577, 1872, 1913, 2659
Bso31I GGTCTC 1 cut(s) 2617
BsoBI CYCGRG 2 cut(s) 397, 3246
Bsp119I TTCGAA 2 cut(s) 2652, 3221
Bsp120I GGGCCC 1 cut(s) 2657
Bsp1286I GDGCHC 5 cut(s) 1267, 1435, 1660, 2661, 3723
Bsp1407I TGTACA 1 cut(s) 3598
Bsp1720I GCTNAGC 1 cut(s) 3213
Bsp19I CCATGG 2 cut(s) 961, 3685
BspACI CCGC 7 cut(s) 123, 336, 466, 669, 2193, 3521, 3538
BspANI GGCC 4 cut(s) 1577, 1872, 1913, 2659
BspCNI CTCAG 8 cut(s) 74, 1324, 1473, 1525, 2131, 2437, 2781, 2859
BspFNI CGCG 1 cut(s) 2374
BspHI TCATGA 3 cut(s) 714, 979, 3529
BspMAI CTGCAG 1 cut(s) 3764
BspOI GCTAGC 1 cut(s) 2586
BspPI GGATC 8 cut(s) 278, 590, 603, 1340, 1402, 1994, 2870, 3248
BspT104I TTCGAA 2 cut(s) 2652, 3221
BspT107I GGYRCC 1 cut(s) 3120
BspTNI GGTCTC 1 cut(s) 2617
BsrDI GCAATG 5 cut(s) 679, 1349, 2047, 2322, 2439
BsrFI RCCGGY 3 cut(s) 41, 1618, 1971
BsrGI TGTACA 1 cut(s) 3598
BsrI ACTGG 6 cut(s) 109, 807, 1561, 1590, 3226, 3592
BssAI RCCGGY 3 cut(s) 41, 1618, 1971
BssECI CCNNGG 5 cut(s) 873, 961, 1113, 3067, 3685
BssNAI GTATAC 1 cut(s) 3357
BssNI GRCGYC 1 cut(s) 78
BssSI CACGAG 3 cut(s) 453, 2040, 3088
BssT1I CCWWGG 5 cut(s) 873, 961, 1113, 3067, 3685
Bst1107I GTATAC 1 cut(s) 3357
Bst2BI CACGAG 3 cut(s) 453, 2040, 3088
Bst2UI CCWGG 2 cut(s) 3083, 3579
Bst4CI ACNGT 5 cut(s) 58, 740, 2319, 3431, 3672
Bst6I CTCTTC 4 cut(s) 155, 2559, 3003, 3895
BstACI GRCGYC 1 cut(s) 78
BstAPI GCANNNNNTGC 3 cut(s) 2510, 3455, 3472
BstAUI TGTACA 1 cut(s) 3598
BstBAI YACGTR 1 cut(s) 3354
BstBI TTCGAA 2 cut(s) 2652, 3221
BstDSI CCRYGG 2 cut(s) 961, 3685
BstF5I GGATG 8 cut(s) 1009, 1586, 2124, 2683, 3574, 3703, 3841, 3874
BstFNI CGCG 1 cut(s) 2374
BstH2I RGCGCY 1 cut(s) 2838
BstHHI GCGC 2 cut(s) 2267, 2837
BstMAI GTCTC 6 cut(s) 64, 902, 960, 2384, 2617, 2850
BstNI CCWGG 2 cut(s) 3083, 3579
BstNSI RCATGY 6 cut(s) 619, 1418, 1841, 1988, 3446, 3458
BstPAI GACNNNNGTC 2 cut(s) 56, 3590
BstSCI CCNGG 4 cut(s) 208, 2569, 3081, 3577
BstSFI CTRYAG 1 cut(s) 3760
BstSLI GKGCMC 1 cut(s) 2661
BstSNI TACGTA 1 cut(s) 3354
BstUI CGCG 1 cut(s) 2374
BstV1I GCAGC 8 cut(s) 17, 648, 2095, 2488, 2758, 3426, 3433, 3851
BstV2I GAAGAC 2 cut(s) 1125, 1428
BstX2I RGATCY 5 cut(s) 595, 1345, 1484, 3253, 3896
BstXI CCANNNNNNTGG 1 cut(s) 272
BstYI RGATCY 5 cut(s) 595, 1345, 1484, 3253, 3896
BstZ17I GTATAC 1 cut(s) 3357
BsuI GTATCC 1 cut(s) 3628
BsuRI GGCC 4 cut(s) 1577, 1872, 1913, 2659
BtgI CCRYGG 2 cut(s) 961, 3685
BtsCI GGATG 8 cut(s) 1009, 1586, 2124, 2683, 3574, 3703, 3841, 3874
BtsI GCAGTG 3 cut(s) 1944, 3411, 3757
BtsIMutI CAGTG 7 cut(s) 800, 1597, 1738, 1944, 2786, 3411, 3757
CaiI CAGNNNCTG 1 cut(s) 361
CciI TCATGA 3 cut(s) 714, 979, 3529
CfoI GCGC 2 cut(s) 2267, 2837
Cfr10I RCCGGY 3 cut(s) 41, 1618, 1971
Cfr13I GGNCC 9 cut(s) 28, 229, 358, 1912, 2302, 2657, 2658, 2986, 3523
CseI GACGC 3 cut(s) 86, 1784, 3699
CsiI ACCWGGT 1 cut(s) 3081
Csp6I GTAC 4 cut(s) 33, 1490, 3296, 3599
CspAI ACCGGT 1 cut(s) 1618
CspCI CAANNNNNGTGG 2 cut(s) 2775, 2810
CviQI GTAC 4 cut(s) 33, 1490, 3296, 3599
DraI TTTAAA 1 cut(s) 2829
DraIII CACNNNGTG 1 cut(s) 3472
EaeI YGGCCR 2 cut(s) 1575, 1870
Eam1104I CTCTTC 4 cut(s) 155, 2559, 3003, 3895
EarI CTCTTC 4 cut(s) 155, 2559, 3003, 3895
Ecl136II GAGCTC 1 cut(s) 3721
Eco105I TACGTA 1 cut(s) 3354
Eco130I CCWWGG 5 cut(s) 873, 961, 1113, 3067, 3685
Eco24I GRGCYC 2 cut(s) 2661, 3723
Eco31I GGTCTC 1 cut(s) 2617
Eco32I GATATC 1 cut(s) 2864
Eco47I GGWCC 6 cut(s) 28, 229, 358, 2302, 2986, 3523
Eco53kI GAGCTC 1 cut(s) 3721
Eco57I CTGAAG 2 cut(s) 746, 2369
Eco88I CYCGRG 2 cut(s) 397, 3246
EcoICRI GAGCTC 1 cut(s) 3721
EcoO109I RGGNCCY 3 cut(s) 358, 2657, 2658
EcoRI GAATTC 3 cut(s) 547, 941, 2134
EcoRII CCWGG 2 cut(s) 3081, 3577
EcoRV GATATC 1 cut(s) 2864
EcoT14I CCWWGG 5 cut(s) 873, 961, 1113, 3067, 3685
EcoT22I ATGCAT 2 cut(s) 1279, 2934
EcoT38I GRGCYC 2 cut(s) 2661, 3723
ErhI CCWWGG 5 cut(s) 873, 961, 1113, 3067, 3685
Esp3I CGTCTC 1 cut(s) 64
FaqI GGGAC 4 cut(s) 215, 623, 1621, 3598
FauI CCCGC 1 cut(s) 130
FbaI TGATCA 3 cut(s) 3568, 3646, 3849
FblI GTMKAC 1 cut(s) 3356
FokI GGATG 8 cut(s) 1016, 1573, 2111, 2670, 3561, 3690, 3848, 3881
FriOI GRGCYC 2 cut(s) 2661, 3723
FspBI CTAG 9 cut(s) 221, 516, 552, 666, 1806, 2078, 2112, 2583, 3105
GlaI GCGC 2 cut(s) 2266, 2836
GsuI CTGGAG 5 cut(s) 2222, 3192, 3217, 3495, 3600
HaeII RGCGCY 1 cut(s) 2838
HaeIII GGCC 4 cut(s) 1577, 1872, 1913, 2659
HapII CCGG 8 cut(s) 42, 209, 1619, 1873, 1972, 2571, 2610, 3380
HgaI GACGC 3 cut(s) 86, 1784, 3699
HhaI GCGC 2 cut(s) 2267, 2837
Hin1I GRCGYC 1 cut(s) 78
Hin6I GCGC 2 cut(s) 2265, 2835
HinP1I GCGC 2 cut(s) 2265, 2835
HincII GTYRAC 3 cut(s) 73, 2151, 3331
HindII GTYRAC 3 cut(s) 73, 2151, 3331
HindIII AAGCTT 4 cut(s) 637, 2117, 2345, 3215
HpaI GTTAAC 1 cut(s) 3331
HpaII CCGG 8 cut(s) 42, 209, 1619, 1873, 1972, 2571, 2610, 3380
HphI GGTGA 5 cut(s) 173, 224, 592, 1775, 3674
Hpy99I CGWCG 1 cut(s) 80
HpyCH4III ACNGT 5 cut(s) 58, 740, 2319, 3431, 3672
HpyCH4IV ACGT 4 cut(s) 2171, 3353, 3359, 3468
HpySE526I ACGT 4 cut(s) 2171, 3353, 3359, 3468
Hsp92I GRCGYC 1 cut(s) 78
HspAI GCGC 2 cut(s) 2265, 2835
KroI GCCGGC 1 cut(s) 1971
KroNI GCCGGC 1 cut(s) 1973
Ksp22I TGATCA 3 cut(s) 3568, 3646, 3849
KspAI GTTAAC 1 cut(s) 3331
LmnI GCTCC 8 cut(s) 317, 793, 850, 1928, 2229, 3211, 3514, 3718
Lsp1109I GCAGC 8 cut(s) 17, 648, 2095, 2488, 2758, 3426, 3433, 3851
MabI ACCWGGT 1 cut(s) 3081
MaeI CTAG 9 cut(s) 221, 516, 552, 666, 1806, 2078, 2112, 2583, 3105
MaeII ACGT 4 cut(s) 2171, 3353, 3359, 3468
MaeIII GTNAC 8 cut(s) 1763, 1940, 2435, 2548, 3085, 3265, 3360, 3491
MfeI CAATTG 3 cut(s) 2268, 2709, 2958
MflI RGATCY 5 cut(s) 595, 1345, 1484, 3253, 3896
MhlI GDGCHC 5 cut(s) 1267, 1435, 1660, 2661, 3723
MlsI TGGCCA 1 cut(s) 1577
MluI ACGCGT 1 cut(s) 2372
MluNI TGGCCA 1 cut(s) 1577
MlyI GAGTC 7 cut(s) 19, 190, 584, 1544, 1737, 2420, 3881
MmeI TCCRAC 4 cut(s) 409, 771, 1697, 2515
Mox20I TGGCCA 1 cut(s) 1577
Mph1103I ATGCAT 2 cut(s) 1279, 2934
MroNI GCCGGC 1 cut(s) 1971
MroXI GAANNNNTTC 3 cut(s) 1122, 2382, 3227
MscI TGGCCA 1 cut(s) 1577
MslI CAYNNNNRTG 7 cut(s) 688, 1133, 1989, 2373, 2544, 2727, 3834
Msp20I TGGCCA 1 cut(s) 1577
MspI CCGG 8 cut(s) 42, 209, 1619, 1873, 1972, 2571, 2610, 3380
MspR9I CCNGG 4 cut(s) 210, 2571, 3083, 3579
MssI GTTTAAAC 1 cut(s) 2829
MunI CAATTG 3 cut(s) 2268, 2709, 2958
Mva1269I GAATGC 4 cut(s) 904, 2648, 2932, 3789
MvaI CCWGG 2 cut(s) 3083, 3579
MvnI CGCG 1 cut(s) 2374
NaeI GCCGGC 1 cut(s) 1973
NciI CCSGG 2 cut(s) 210, 2571
NcoI CCATGG 2 cut(s) 961, 3685
NgoMIV GCCGGC 1 cut(s) 1971
NheI GCTAGC 1 cut(s) 2582
NmuCI GTSAC 4 cut(s) 1763, 1940, 3085, 3360
NsiI ATGCAT 2 cut(s) 1279, 2934
NspI RCATGY 6 cut(s) 619, 1418, 1841, 1988, 3446, 3458
NspV TTCGAA 2 cut(s) 2652, 3221
PaeI GCATGC 1 cut(s) 3446
PagI TCATGA 3 cut(s) 714, 979, 3529
PciI ACATGT 1 cut(s) 1984
PctI GAATGC 4 cut(s) 904, 2648, 2932, 3789
PdiI GCCGGC 1 cut(s) 1973
PdmI GAANNNNTTC 3 cut(s) 1122, 2382, 3227
PflMI CCANNNNNTGG 1 cut(s) 605
PfoI TCCNGGA 2 cut(s) 2569, 3577
PinAI ACCGGT 1 cut(s) 1618
PleI GAGTC 7 cut(s) 18, 189, 583, 1543, 1736, 2419, 3881
PmeI GTTTAAAC 1 cut(s) 2829
PpsI GAGTC 7 cut(s) 18, 189, 583, 1543, 1736, 2419, 3881
Ppu21I YACGTR 1 cut(s) 3354
PpuMI RGGWCCY 1 cut(s) 358
PscI ACATGT 1 cut(s) 1984
PshAI GACNNNNGTC 2 cut(s) 56, 3590
PsiI TTATAA 1 cut(s) 1923
Psp124BI GAGCTC 1 cut(s) 3723
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 2 cut(s) 3081, 3577
PspGI CCWGG 2 cut(s) 3081, 3577
PspOMI GGGCCC 1 cut(s) 2657
PspPI GGNCC 9 cut(s) 28, 229, 358, 1912, 2302, 2657, 2658, 2986, 3523
PspPPI RGGWCCY 1 cut(s) 358
PstI CTGCAG 1 cut(s) 3764
PstNI CAGNNNCTG 1 cut(s) 361
PsuI RGATCY 5 cut(s) 595, 1345, 1484, 3253, 3896
RsaI GTAC 4 cut(s) 34, 1491, 3297, 3600
RsaNI GTAC 4 cut(s) 33, 1490, 3296, 3599
RseI CAYNNNNRTG 7 cut(s) 688, 1133, 1989, 2373, 2544, 2727, 3834
SacI GAGCTC 1 cut(s) 3723
Sau96I GGNCC 9 cut(s) 28, 229, 358, 1912, 2302, 2657, 2658, 2986, 3523
SchI GAGTC 7 cut(s) 19, 190, 584, 1544, 1737, 2420, 3881
ScrFI CCNGG 4 cut(s) 210, 2571, 3083, 3579
SduI GDGCHC 5 cut(s) 1267, 1435, 1660, 2661, 3723
SexAI ACCWGGT 1 cut(s) 3081
SfcI CTRYAG 1 cut(s) 3760
SfuI TTCGAA 2 cut(s) 2652, 3221
SinI GGWCC 6 cut(s) 28, 229, 358, 2302, 2986, 3523
SmiMI CAYNNNNRTG 7 cut(s) 688, 1133, 1989, 2373, 2544, 2727, 3834
SmlI CTYRAG 2 cut(s) 92, 1689
SmoI CTYRAG 2 cut(s) 92, 1689
SnaBI TACGTA 1 cut(s) 3354
SphI GCATGC 1 cut(s) 3446
SsiI CCGC 7 cut(s) 123, 336, 466, 669, 2193, 3521, 3538
SspI AATATT 2 cut(s) 1324, 2672
SspMI CTAG 9 cut(s) 221, 516, 552, 666, 1806, 2078, 2112, 2583, 3105
SstI GAGCTC 1 cut(s) 3723
StyD4I CCNGG 4 cut(s) 208, 2569, 3081, 3577
StyI CCWWGG 5 cut(s) 873, 961, 1113, 3067, 3685
TaaI ACNGT 5 cut(s) 58, 740, 2319, 3431, 3672
TaiI ACGT 4 cut(s) 2174, 3356, 3362, 3471
TaqI TCGA 9 cut(s) 24, 184, 383, 1419, 1435, 1538, 2652, 3006, 3221
TatI WGTACW 3 cut(s) 1489, 3295, 3598
TauI GCSGC 2 cut(s) 672, 3540
TscAI CASTG 7 cut(s) 807, 1597, 1738, 1951, 2793, 3411, 3764
TseFI GTSAC 4 cut(s) 1763, 1940, 3085, 3360
TseI GCWGC 8 cut(s) 5, 661, 2108, 2501, 2771, 3439, 3446, 3839
Tsp45I GTSAC 4 cut(s) 1763, 1940, 3085, 3360
TspGWI ACGGA 2 cut(s) 20, 58
TspRI CASTG 7 cut(s) 807, 1597, 1738, 1951, 2793, 3411, 3764
Van91I CCANNNNNTGG 1 cut(s) 605
VpaK11BI GGWCC 6 cut(s) 28, 229, 358, 2302, 2986, 3523
XapI RAATTY 7 cut(s) 269, 547, 941, 1865, 2065, 2134, 2379
XbaI TCTAGA 2 cut(s) 551, 2077
XceI RCATGY 6 cut(s) 619, 1418, 1841, 1988, 3446, 3458
XcmI CCANNNNNNNNNTGG 1 cut(s) 1865
XmiI GTMKAC 1 cut(s) 3356
XmnI GAANNNNTTC 3 cut(s) 1122, 2382, 3227
XspI CTAG 9 cut(s) 221, 516, 552, 666, 1806, 2078, 2112, 2583, 3105
Zsp2I ATGCAT 2 cut(s) 1279, 2934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.