Rorug06G0459500

ATP-dependent helicase hrq1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006845
Physical Location & Seq
Forward (+)
58636528 .. 58638711
2184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0459500.1

Sequence Viewer

Length: 522 bp
ATGGAGAGACTTGCTAGGTATCAAAGGGTCATAGCTGGGTCTCCCAGGGAAGCAAACAAGTTAGAGAACAAGTTGGGTAGGTACCTTACCACAACTTTGGGAATTCACGAAGATGAGCAACCGTTTATTACTGTAAAAATATTGGAAATGCTCCGAGAAGCCGTCCCCGATAAAATTATAGAAGTGCACTTGATAGAAATTACATTTAGAAGTACGAATTGGTCCTTTCTGTGGGGCTTCAGCGAGGACGGGGACGATATATACTACAGGGCTAAGCTGGTTCCTGCTCCGAAATCATTCATCGAGTCTCTGGAGGAAGTGAGACTACTTGATGATGATGATAATCTGGAATTGCTTGGATTGGAGTGTCCTATTTGTATGCAGGTGTTTGCAGAGAAAAAAGATACCAGCATTACTCGGTTGCCATGCTCACACTATTACCATCGAGATTGCATTGTCCAGTGGCTCCAGATCAATCATGTCTGTCCCACTTGTCGACACCCTAATGCTCCGATGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DUF1998 Helicase_C

Protein Analysis

173

Amino Acids

20.16

Weight (kDa)

5.37

Isoelectric Point (pI)

53.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 122 - 166 1.7e-13 Ring finger domain
zf-RING_11 PF17123 122 - 152 5e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 122 - 165 1.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 123 - 165 1.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 123 - 163 2.4e-06 RING-type zinc-finger
zf-rbx1 PF12678 126 - 166 1.6e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 373
Acc36I ACCTGC 1 cut(s) 373
Acc65I GGTACC 1 cut(s) 81
AccB1I GGYRCC 1 cut(s) 81
AccI GTMKAC 1 cut(s) 496
AcsI RAATTY 1 cut(s) 102
AcuI CTGAAG 1 cut(s) 223
AfaI GTAC 2 cut(s) 83, 214
AfiI CCNNNNNNNGG 1 cut(s) 231
AjnI CCWGG 1 cut(s) 44
AjuI GAANNNNNNNTTGG 2 cut(s) 202, 234
AluBI AGCT 2 cut(s) 35, 277
AluI AGCT 2 cut(s) 35, 277
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 3 cut(s) 45, 312, 316
Alw44I GTGCAC 1 cut(s) 185
ApaLI GTGCAC 1 cut(s) 185
ApoI RAATTY 1 cut(s) 102
Asp700I GAANNNNTTC 1 cut(s) 296
Asp718I GGTACC 1 cut(s) 81
AspS9I GGNCC 1 cut(s) 222
AvaII GGWCC 1 cut(s) 222
BaeGI GKGCMC 1 cut(s) 189
BanI GGYRCC 1 cut(s) 81
BarI GAAGNNNNNNTAC 2 cut(s) 309, 341
Bbv12I GWGCWC 1 cut(s) 189
BccI CCATC 1 cut(s) 450
BceAI ACGGC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 46
BcoDI GTCTC 3 cut(s) 45, 312, 316
BfaI CTAG 1 cut(s) 15
BfmI CTRYAG 1 cut(s) 265
BfuAI ACCTGC 1 cut(s) 373
BlpI GCTNAGC 1 cut(s) 273
Bme1390I CCNGG 1 cut(s) 46
Bme18I GGWCC 1 cut(s) 222
BmgT120I GGNCC 1 cut(s) 222
BmiI GGNNCC 3 cut(s) 83, 282, 467
BmrFI CCNGG 1 cut(s) 46
BpmI CTGGAG 2 cut(s) 332, 452
Bpu1102I GCTNAGC 1 cut(s) 273
BsaBI GATNNNNATC 1 cut(s) 342
BsaI GGTCTC 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 44, 45
Bsc4I CCNNNNNNNGG 1 cut(s) 231
Bse1I ACTGG 1 cut(s) 460
Bse8I GATNNNNATC 1 cut(s) 342
BseBI CCWGG 1 cut(s) 46
BseDI CCNNGG 2 cut(s) 44, 45
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 1 cut(s) 231
BseNI ACTGG 1 cut(s) 460
BseSI GKGCMC 1 cut(s) 189
BseYI CCCAGC 1 cut(s) 35
BshNI GGYRCC 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 189
BslFI GGGAC 3 cut(s) 149, 266, 471
BslI CCNNNNNNNGG 1 cut(s) 231
BsmAI GTCTC 3 cut(s) 45, 312, 316
BsmFI GGGAC 3 cut(s) 149, 266, 471
Bso31I GGTCTC 1 cut(s) 45
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 1 cut(s) 471
Bsp1720I GCTNAGC 1 cut(s) 273
BspLI GGNNCC 3 cut(s) 83, 282, 467
BspMI ACCTGC 1 cut(s) 373
BspT107I GGYRCC 1 cut(s) 81
BspTNI GGTCTC 1 cut(s) 45
BsrI ACTGG 1 cut(s) 460
BssECI CCNNGG 2 cut(s) 44, 45
BssMI GATC 1 cut(s) 471
Bst2UI CCWGG 1 cut(s) 46
Bst4CI ACNGT 2 cut(s) 123, 133
BstDEI CTNAG 1 cut(s) 273
BstKTI GATC 1 cut(s) 474
BstMAI GTCTC 3 cut(s) 45, 312, 316
BstMBI GATC 1 cut(s) 471
BstNI CCWGG 1 cut(s) 46
BstSCI CCNGG 1 cut(s) 44
BstSFI CTRYAG 1 cut(s) 265
BstSLI GKGCMC 1 cut(s) 189
BstXI CCANNNNNNTGG 1 cut(s) 97
BtsIMutI CAGTG 1 cut(s) 467
BveI ACCTGC 1 cut(s) 373
Cfr13I GGNCC 1 cut(s) 222
Csp6I GTAC 2 cut(s) 82, 213
CviAII CATG 2 cut(s) 426, 479
CviJI RGCY 6 cut(s) 35, 161, 237, 272, 277, 466
CviKI_1 RGCY 6 cut(s) 35, 161, 237, 272, 277, 466
CviQI GTAC 2 cut(s) 82, 213
DdeI CTNAG 1 cut(s) 273
DpnI GATC 1 cut(s) 473
DpnII GATC 1 cut(s) 471
Eco31I GGTCTC 1 cut(s) 45
Eco47I GGWCC 1 cut(s) 222
Eco57I CTGAAG 1 cut(s) 223
EcoRI GAATTC 1 cut(s) 102
EcoRII CCWGG 1 cut(s) 44
FaeI CATG 2 cut(s) 429, 482
FaiI YATR 7 cut(s) 32, 179, 260, 262, 380, 427, 480
FaqI GGGAC 3 cut(s) 149, 266, 471
FatI CATG 2 cut(s) 425, 478
FblI GTMKAC 1 cut(s) 496
FspBI CTAG 1 cut(s) 15
GsaI CCCAGC 1 cut(s) 39
GsuI CTGGAG 2 cut(s) 332, 452
Hin1II CATG 2 cut(s) 429, 482
HincII GTYRAC 1 cut(s) 497
HindII GTYRAC 1 cut(s) 497
HinfI GANTC 1 cut(s) 305
Hpy166II GTNNAC 2 cut(s) 187, 497
Hpy188I TCNGA 3 cut(s) 155, 291, 513
Hpy188III TCNNGA 5 cut(s) 107, 311, 347, 446, 469
Hpy8I GTNNAC 2 cut(s) 187, 497
HpyCH4III ACNGT 2 cut(s) 123, 133
HpyCH4V TGCA 4 cut(s) 187, 382, 392, 453
HpyF3I CTNAG 1 cut(s) 273
Hsp92II CATG 2 cut(s) 429, 482
KpnI GGTACC 1 cut(s) 85
Kzo9I GATC 1 cut(s) 471
LmnI GCTCC 4 cut(s) 156, 292, 471, 514
MaeI CTAG 1 cut(s) 15
MalI GATC 1 cut(s) 473
MboI GATC 1 cut(s) 471
MboII GAAGA 1 cut(s) 122
MhlI GDGCHC 1 cut(s) 189
MluCI AATT 5 cut(s) 102, 174, 198, 217, 350
MlyI GAGTC 1 cut(s) 314
MnlI CCTC 2 cut(s) 238, 307
MroXI GAANNNNTTC 1 cut(s) 296
MslI CAYNNNNRTG 2 cut(s) 111, 504
MspR9I CCNGG 1 cut(s) 46
MvaI CCWGG 1 cut(s) 46
NdeII GATC 1 cut(s) 471
NlaIII CATG 2 cut(s) 429, 482
NlaIV GGNNCC 3 cut(s) 83, 282, 467
PaqCI CACCTGC 1 cut(s) 373
PasI CCCWGGG 1 cut(s) 45
PdmI GAANNNNTTC 1 cut(s) 296
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
Psp6I CCWGG 1 cut(s) 44
PspFI CCCAGC 1 cut(s) 35
PspGI CCWGG 1 cut(s) 44
PspN4I GGNNCC 3 cut(s) 83, 282, 467
PspPI GGNCC 1 cut(s) 222
RsaI GTAC 2 cut(s) 83, 214
RsaNI GTAC 2 cut(s) 82, 213
RseI CAYNNNNRTG 2 cut(s) 111, 504
SalI GTCGAC 1 cut(s) 495
Sau3AI GATC 1 cut(s) 471
Sau96I GGNCC 1 cut(s) 222
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 1 cut(s) 46
SduI GDGCHC 1 cut(s) 189
SetI ASST 6 cut(s) 20, 37, 83, 87, 279, 387
SfcI CTRYAG 1 cut(s) 265
SinI GGWCC 1 cut(s) 222
SmiMI CAYNNNNRTG 2 cut(s) 111, 504
Sse9I AATT 5 cut(s) 102, 174, 198, 217, 350
SspI AATATT 1 cut(s) 141
SspMI CTAG 1 cut(s) 15
StyD4I CCNGG 1 cut(s) 44
TaaI ACNGT 2 cut(s) 123, 133
TaqI TCGA 3 cut(s) 303, 445, 496
TasI AATT 5 cut(s) 102, 174, 198, 217, 350
TscAI CASTG 1 cut(s) 467
TspDTI ATGAA 1 cut(s) 289
TspRI CASTG 1 cut(s) 467
VneI GTGCAC 1 cut(s) 185
VpaK11BI GGWCC 1 cut(s) 222
XapI RAATTY 1 cut(s) 102
XmiI GTMKAC 1 cut(s) 496
XmnI GAANNNNTTC 1 cut(s) 296
XspI CTAG 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.