MD14G1216300.v1.1

ATP-dependent helicase hrq1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006845
Physical Location & Seq
Reverse (-)
29980251 .. 29989564
9314 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1216300.v1.1.491

Sequence Viewer

Length: 3819 bp
ATGGCGGAAGGCCAGATTGAGATCCAGGTCCGTGCGCTGGAAGGCAAACCGGCCACGGTTTCGGTCCCGGTCGACGAAACCATCGACGGCCTCAAGCTCCTTCTCAAGCAATTTTTCCCACCCGCCAAAAACTCCCCCAATTTTCACCTTTTCTTCAAGGGTGAGAAGTTGAGGCTGCAGAGTCAGATTGGAAGGCTCAACATTCGTCCGGGTGAGTTTCTAGTGCTTGCCCCATTTACTAAGAAAGACCCGAATTGTAATGAGACCCCGAAATCTGAACAAACGAAAACACCAGCGAATTGTCCTAGTAAAAGCTCGATTAAGAAGTTTGCCGATTCGGCGTGGTCTGATATGATGGAAGACTTAACCTTTTTACATGACAATTCAAATGATAAAACTCCGAAGGGGCCTAGTGTAGGAAGTTTCAATCTTGGAGGTAGAAGTGAGGTAAATGATGAAGCCGCTAGTTCTCGCGCTTGTGGAGCAAAGAGAAAAATGGGAACTTTTGATTGTGATGACATTGTATTGGATATATTGCGTTCTAGTATAAACGGAAATGTCCTTGATGAACAGAATTTTAAAAGGTTTATTGAGGTTTTGGAGACGGTGAGTTGTTTATCAGACCCTTATTCCCGGGACTGCTTGTTGTGGAGAAGAGCTGTTTTGCGGCAACAGGGCTTTGGCACTGGACTGCATAAAGGCAATAGTAGTTCATGCTTGTGTCCAGCGTGGTTGAAGATAATCATGAAGGCATTTGCTTTCTTAAACACTTTTTCTGCATTTCTTCAATTACAGCAGGCGAGAACAACCTCAATCATTTTGGAGCAAGCATTGCACCAGCTTGGGAGATTTGGAGTTAGGCTTGGTATGAAGGACATAGAACATCTTTCCATTATTTCTCCTAAGGTAGTACATTTTGCAAATGAGAATGCAGAGCTGGAAGGTTCTGGTAATGTGCTTGTTATTATCAATGGTTCAACAGGACAGAATGGTCGAAATGAAGATCATCATAAATATGCTTGCAAGCAAATGGATATTTCAAAGATTATCAGTATATCGAAAATACGGGAAAGTTCTTTCAGAAGTAATTTGTGGGAGGCTGTAGAGTTGTTAATGTTTAAAACTGGAAATGAGACTTCCATGGATTTTTCCTTGGAAGACTTGCTCAAATCAGTGAAGGAATGTGGTCCTGCTGCAAGAGGAAATGAAGCAAAGCGACTAAGGGTAACACGTGCAGCTTCAAATAATTATTTTGACCGAAATCAATCTAAAGCTTCAAGAAAACATCGATGTTGTTTAACAGGTTCATTCCAACCAGAGGAGATGATTAAACATCTTAGAGAGGGACTTGGATCGAAAGGACAGATAGTGCATGTTGAAGACATTAGTGCTCGGAGAGCCGCTTATGTAGAAGTTCTACAAGAACTATCTGAAAACATGAGATCTGCACTTCGTAGCACTGGAATCACTCAATTATATACTCATCAGGCAGAGTCAATACAAGCCTCACTTTCTGGGAAGAATGTTGTTGTGGCAACAATGACATCGAGTGGGAAATCTCTTTGCTACAATCTGCCGGTCTTAGAAGCCTTGTCCCACAGTTCATCTGCATGCGCTCTATATTTATTTCCAACAAAGGCTTTAGCTCAGGATCAACTAAGAAGTTTGTTGGCTATGACCAAAGGATTCAATTGTGACTTAAACATTGGTGTATATGACGGTGACACTCCTCAGGAGGAGAGGACATGGCTGCGTCAAAATGCTAGGCTGCTAATCACAAATCCAGATATGTTGCATGTATCACTCCTGCCATACCATAAAGGATTTCGGCGGATTTTGGAAAATCTCAGGTTTGTAGTCATTGATGAGGCACATGTGTATAAGGGGGCATTTGGTTGTCATACTGCTCTAATATTAAGGAGACTTCGCCGGCTCTGCTCACATGTATATGGAAGTGATCCTTCTTTTGTGTTTTCTACTGCAACTTCGGCAAATCCTCATGATCATTGCATGGAACTTGCTAGTCTACCAACTTTAGAGCTGATTCAGAATGATGGAAGTCCGTCTGCAAGAAAGCTTTTCGTCCTTTGGAATCCTATTGCGAATCTACAAAGACAAACTACGTCGACGACAACCAATAATATTATGGATAATGGTAACTCTGCTGTTGAAAATGCTAATCCCCATACTTCAAGCCCAATGATGGATCTTTCATGCCTCTTCGCTGAAATGGTACAGCATGGTCTACGTTGCATTGCGTTTTGCAAATCACGCAAGCTTTGTGAACTTGTGTTGTGCTATACGCGTGAGATTCTTCTGGAGACCGCACCCAATTTAGCGGATTCTATATGTTCTTATCGTGGTGGTTACGTTGCAGAGGATAGGAGAAAGATAGAGAGGGATTTTTTCAATGGAAAACTTTGTGGTATCGCTGCAACAAATGCCCTTGAATTGGGTATTGATGTTGGACACATAGATGCAACTCTTCATTTGGGTTTTCCTGGTAGTATATCTAGCTTGTGGCAACAAGCTGGCAGGGCTGGAAGGAGAGAGAGACATTCTCTTGCAGTGTATGTTGCATTTGAAGGGCCCCTTGATCAATACTTCATGAAACAACCAAATAAACTTTTTGGAAGCCCAATTGAGCGCTGCCATGTCGATGCTAAGAACCAAATGGTTCTTGCACAGCATTTGGCTTGTGCTGCTCTAGAACACCCCCTGAGTTCGTCTTATGATGAAAGGTTTTTCGGCTCTAGTATGAACAGTGCCATAGTACCTCTTAAAAATAAAGGGTATTTGAGTCTTGATCCATCGTGTAATTCGTCTGCCGAGATTTGGAACTATACTGGGCAAGAGAAAATGCCTTCACGTTCAGTAAGCATCCGATCAATAGATTCTGAGAAATACAAAGTTATAGACCAGCAAAAGAAAGAAATCCTTGAAGAGATCGAGGAGGGCAAGGCTTTCTTTCAGGTATATAAAGGTGCTGTTTATATGAACCAAGGAAATACATACCTGGTCACAAGCTTAGATACTTGGAGTAAGGAAGCTTTATGCAAAGCAGCTAAGTTGGATTATTACACAAAACCCCGTGATTGCACAAGAGTTCATATCATGGGCGGTAATATGGCTCATCCACCTAAGGTTTCCAGAACATCTGCCCGAACAGATTCTTGCAAAGTAACAACTAGATGGTTTGGATACTTTAGCATATGTAAAGCAACGCACGAGGTACTTGAATCAGTTGAACTTAAACTTCCGGAATATACTTATGAGTCACAGGCAGTGTGGGTTCCAGTTCCACATTGGATAAGAGAATCAGTGGAGGCGAAAGACCTTGATTTCCGTGCTGGACTGCATGCTGCAACACATGCTGTTCTACACGTTGTGCCTTTACGGATAATATGCAACTTATCTGACTTGGCTCCAGAGTGTCCATACCCTACTGACAAATCCTATTTTCCAGAAAGGATTCTATTATATGACCAGCATCGTGGAGGGACTGGTGTCTCTGTACAGGTTCAACCGATATTTATGGAGCTCTTTACTCCTGCTATCGAACTTCTTTCGTCTTGCAGCTGCTCAAGATCTGGTGGCTGCCCTAACTGTGTACAAAGTTTTGCGTGCGAGGCATATAATGAAGATTTACATAGGGAGACAGCAATTATGATCCTTAAGGTGGGTGTTCTTGAAGAAGAAAACTTAAACACGCGGGGATCTCCCGAGTCATTGAAATCTTCGGTGAAGCAAGACGGAAGTAGAGGAACTGGCAATGGGTACCTACAACCGTCACCGTCACCACCACCACCACATCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DUF1998 Helicase_C

Protein Analysis

1273

Amino Acids

141.61

Weight (kDa)

8.08

Isoelectric Point (pI)

50.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 494 - 664 1.9e-30 DEAD/DEAH box helicase
Helicase_C PF00271 744 - 850 2.2e-13 Helicase conserved C-terminal domain
WHD_HRQ1 PF22982 886 - 964 8.2e-15 ATP-dependent helicase HRQ1, winged helix domain
MZB PF09369 1123 - 1204 8.9e-15 MrfA Zn-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 990
Acc65I GGTACC 1 cut(s) 3777
AccB1I GGYRCC 1 cut(s) 3777
AccI GTMKAC 4 cut(s) 72, 2026, 2126, 2245
AccII CGCG 3 cut(s) 474, 2305, 3712
AccIII TCCGGA 1 cut(s) 3259
AclWI GGATC 8 cut(s) 16, 1360, 1659, 1952, 2214, 2801, 3664, 3724
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 1 cut(s) 574
AcvI CACGTG 1 cut(s) 1232
AdeI CACNNNGTG 1 cut(s) 3388
AfaI GTAC 7 cut(s) 912, 2235, 2775, 3234, 3516, 3612, 3779
AfeI AGCGCT 1 cut(s) 2648
AfiI CCNNNNNNNGG 7 cut(s) 37, 416, 1318, 2203, 2452, 2835, 3679
AflII CTTAAG 1 cut(s) 3674
AflIII ACRYGT 5 cut(s) 1229, 1873, 1942, 2303, 3382
AjnI CCWGG 3 cut(s) 24, 2500, 3015
Alw21I GWGCWC 2 cut(s) 1393, 3543
Alw26I GTCTC 8 cut(s) 257, 596, 1127, 1915, 2315, 2548, 3514, 3650
AlwI GGATC 8 cut(s) 16, 1360, 1659, 1952, 2214, 2801, 3664, 3724
Ama87I CYCGRG 2 cut(s) 633, 3722
Aor13HI TCCGGA 1 cut(s) 3259
Aor51HI AGCGCT 1 cut(s) 2648
AoxI GGCC 5 cut(s) 10, 51, 88, 407, 2588
ApaI GGGCCC 1 cut(s) 2592
ApoI RAATTY 1 cut(s) 574
Asp700I GAANNNNTTC 2 cut(s) 3169, 3471
Asp718I GGTACC 1 cut(s) 3777
AspLEI GCGC 4 cut(s) 37, 476, 1616, 2649
AspS9I GGNCC 6 cut(s) 28, 64, 407, 1187, 2588, 2589
AsuC2I CCSGG 4 cut(s) 68, 210, 634, 635
AsuHPI GGTGA 8 cut(s) 137, 173, 224, 619, 1733, 3754, 3783, 3789
AvaI CYCGRG 2 cut(s) 633, 3722
AvaII GGWCC 3 cut(s) 28, 64, 1187
AxyI CCTNAGG 3 cut(s) 903, 1731, 3141
BaeGI GKGCMC 1 cut(s) 2592
BanI GGYRCC 1 cut(s) 3777
BanII GRGCYC 2 cut(s) 2592, 3543
BauI CACGAG 1 cut(s) 3227
BbrPI CACGTG 1 cut(s) 1232
BbsI GAAGAC 3 cut(s) 366, 1164, 1386
Bbv12I GWGCWC 2 cut(s) 1393, 3543
BccI CCATC 6 cut(s) 89, 349, 2048, 2197, 2818, 3186
BceAI ACGGC 1 cut(s) 103
BciT130I CCWGG 3 cut(s) 26, 2502, 3017
BciVI GTATCC 1 cut(s) 3194
BclI TGATCA 2 cut(s) 2002, 2596
BcnI CCSGG 4 cut(s) 68, 210, 634, 635
BcoDI GTCTC 8 cut(s) 257, 596, 1127, 1915, 2315, 2548, 3514, 3650
BfmI CTRYAG 2 cut(s) 176, 1101
BfoI RGCGCY 1 cut(s) 2650
BfrI CTTAAG 1 cut(s) 3674
BfuI GTATCC 1 cut(s) 3194
BglI GCCNNNNNGGC 1 cut(s) 338
BglII AGATCT 2 cut(s) 1442, 3587
Bme1390I CCNGG 7 cut(s) 26, 68, 210, 634, 635, 2502, 3017
Bme18I GGWCC 3 cut(s) 28, 64, 1187
BmeT110I CYCGRG 2 cut(s) 633, 3722
BmgT120I GGNCC 6 cut(s) 28, 64, 407, 1187, 2588, 2589
BmiI GGNNCC 7 cut(s) 66, 408, 2590, 2591, 3294, 3426, 3779
BmrFI CCNGG 7 cut(s) 26, 68, 210, 634, 635, 2502, 3017
BmrI ACTGGG 1 cut(s) 2856
BmsI GCATC 4 cut(s) 2467, 2650, 2889, 3499
BmuI ACTGGG 1 cut(s) 2856
BoxI GACNNNNGTC 1 cut(s) 3506
BpiI GAAGAC 3 cut(s) 366, 1164, 1386
BplI GAGNNNNNCTC 2 cut(s) 2545, 2577
BpmI CTGGAG 2 cut(s) 2339, 3411
Bpu10I CCTNAGC 1 cut(s) 1647
BpuEI CTTGAG 3 cut(s) 77, 89, 3568
BpuMI CCSGG 4 cut(s) 68, 210, 634, 635
Bsa29I ATCGAT 1 cut(s) 1288
BsaAI YACGTR 1 cut(s) 1232
BsaBI GATNNNNATC 1 cut(s) 20
BsaI GGTCTC 2 cut(s) 257, 2315
BsaJI CCNNGG 5 cut(s) 54, 633, 1140, 1152, 3001
BsaWI WCCGGW 1 cut(s) 3259
Bsc4I CCNNNNNNNGG 7 cut(s) 37, 416, 1318, 2203, 2452, 2835, 3679
Bse118I RCCGGY 3 cut(s) 49, 1576, 1929
Bse1I ACTGG 7 cut(s) 691, 1129, 1465, 2851, 3296, 3508, 3772
Bse21I CCTNAGG 3 cut(s) 903, 1731, 3141
Bse3DI GCAATG 4 cut(s) 830, 2005, 2253, 3778
Bse8I GATNNNNATC 1 cut(s) 20
BseAI TCCGGA 1 cut(s) 3259
BseBI CCWGG 3 cut(s) 26, 2502, 3017
BseCI ATCGAT 1 cut(s) 1288
BseDI CCNNGG 5 cut(s) 54, 633, 1140, 1152, 3001
BseGI GGATG 3 cut(s) 2880, 3133, 3811
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 7 cut(s) 37, 416, 1318, 2203, 2452, 2835, 3679
BseMI GCAATG 4 cut(s) 830, 2005, 2253, 3778
BseMII CTCAG 5 cut(s) 1661, 1745, 1861, 2711, 2889
BseNI ACTGG 7 cut(s) 691, 1129, 1465, 2851, 3296, 3508, 3772
BseRI GAGGAG 4 cut(s) 1334, 1719, 1751, 2966
BseSI GKGCMC 1 cut(s) 2592
BsgI GTGCAG 2 cut(s) 1254, 1431
Bsh1236I CGCG 3 cut(s) 474, 2305, 3712
Bsh1285I CGRYCG 1 cut(s) 72
BshFI GGCC 5 cut(s) 12, 53, 90, 409, 2590
BshNI GGYRCC 1 cut(s) 3777
BshVI ATCGAT 1 cut(s) 1288
BsiEI CGRYCG 1 cut(s) 72
BsiHKAI GWGCWC 2 cut(s) 1393, 3543
BsiHKCI CYCGRG 2 cut(s) 633, 3722
BsiSI CCGG 7 cut(s) 50, 68, 209, 634, 1577, 1930, 3260
BslFI GGGAC 5 cut(s) 50, 650, 1359, 1579, 3514
BslI CCNNNNNNNGG 7 cut(s) 37, 416, 1318, 2203, 2452, 2835, 3679
BsmAI GTCTC 8 cut(s) 257, 596, 1127, 1915, 2315, 2548, 3514, 3650
BsmBI CGTCTC 1 cut(s) 596
BsmFI GGGAC 5 cut(s) 50, 650, 1359, 1579, 3514
BsmI GAATGC 1 cut(s) 934
BsnI GGCC 5 cut(s) 12, 53, 90, 409, 2590
Bso31I GGTCTC 2 cut(s) 257, 2315
BsoBI CYCGRG 2 cut(s) 633, 3722
Bsp120I GGGCCC 1 cut(s) 2588
Bsp1286I GDGCHC 3 cut(s) 1393, 2592, 3543
Bsp13I TCCGGA 1 cut(s) 3259
Bsp1407I TGTACA 2 cut(s) 3514, 3610
Bsp19I CCATGG 1 cut(s) 1140
BspANI GGCC 5 cut(s) 12, 53, 90, 409, 2590
BspCNI CTCAG 5 cut(s) 1660, 1744, 1860, 2712, 2890
BspDI ATCGAT 1 cut(s) 1288
BspEI TCCGGA 1 cut(s) 3259
BspFNI CGCG 3 cut(s) 474, 2305, 3712
BspHI TCATGA 3 cut(s) 744, 1999, 2607
BspLI GGNNCC 7 cut(s) 66, 408, 2590, 2591, 3294, 3426, 3779
BspMAI CTGCAG 1 cut(s) 180
BspPI GGATC 8 cut(s) 16, 1360, 1659, 1952, 2214, 2801, 3664, 3724
BspQI GCTCTTC 1 cut(s) 649
BspT107I GGYRCC 1 cut(s) 3777
BspTI CTTAAG 1 cut(s) 3674
BspTNI GGTCTC 2 cut(s) 257, 2315
BsrDI GCAATG 4 cut(s) 830, 2005, 2253, 3778
BsrFI RCCGGY 3 cut(s) 49, 1576, 1929
BsrGI TGTACA 2 cut(s) 3514, 3610
BsrI ACTGG 7 cut(s) 691, 1129, 1465, 2851, 3296, 3508, 3772
BssAI RCCGGY 3 cut(s) 49, 1576, 1929
BssECI CCNNGG 5 cut(s) 54, 633, 1140, 1152, 3001
BssSI CACGAG 1 cut(s) 3227
BssT1I CCWWGG 3 cut(s) 1140, 1152, 3001
Bst2BI CACGAG 1 cut(s) 3227
Bst2UI CCWGG 3 cut(s) 26, 2502, 3017
Bst4CI ACNGT 8 cut(s) 58, 607, 1601, 1721, 2765, 3608, 3789, 3795
Bst6I CTCTTC 4 cut(s) 649, 2225, 2490, 2937
BstAFI CTTAAG 1 cut(s) 3674
BstAPI GCANNNNNTGC 3 cut(s) 832, 2441, 3371
BstAUI TGTACA 2 cut(s) 3514, 3610
BstBAI YACGTR 1 cut(s) 1232
BstDSI CCRYGG 2 cut(s) 54, 1140
BstENI CCTNNNNNAGG 1 cut(s) 414
BstF5I GGATG 3 cut(s) 2880, 3133, 3811
BstFNI CGCG 3 cut(s) 474, 2305, 3712
BstH2I RGCGCY 1 cut(s) 2650
BstHHI GCGC 4 cut(s) 37, 476, 1616, 2649
BstMAI GTCTC 8 cut(s) 257, 596, 1127, 1915, 2315, 2548, 3514, 3650
BstMCI CGRYCG 1 cut(s) 72
BstNI CCWGG 3 cut(s) 26, 2502, 3017
BstNSI RCATGY 7 cut(s) 1376, 1614, 1799, 1877, 1946, 3362, 3374
BstPAI GACNNNNGTC 1 cut(s) 3506
BstSCI CCNGG 7 cut(s) 24, 66, 208, 632, 633, 2500, 3015
BstSFI CTRYAG 2 cut(s) 176, 1101
BstSLI GKGCMC 1 cut(s) 2592
BstUI CGCG 3 cut(s) 474, 2305, 3712
BstV2I GAAGAC 3 cut(s) 366, 1164, 1386
BstX2I RGATCY 5 cut(s) 21, 1442, 2206, 3587, 3716
BstXI CCANNNNNNTGG 1 cut(s) 3494
BstYI RGATCY 5 cut(s) 21, 1442, 2206, 3587, 3716
Bsu15I ATCGAT 1 cut(s) 1288
Bsu36I CCTNAGG 3 cut(s) 903, 1731, 3141
BsuI GTATCC 1 cut(s) 3194
BsuRI GGCC 5 cut(s) 12, 53, 90, 409, 2590
BsuTUI ATCGAT 1 cut(s) 1288
BtgI CCRYGG 2 cut(s) 54, 1140
BtsCI GGATG 3 cut(s) 2880, 3133, 3811
BtsI GCAGTG 2 cut(s) 2574, 3291
BtsIMutI CAGTG 7 cut(s) 684, 1179, 1458, 2574, 2770, 3291, 3327
CciI TCATGA 3 cut(s) 744, 1999, 2607
CfoI GCGC 4 cut(s) 37, 476, 1616, 2649
Cfr10I RCCGGY 3 cut(s) 49, 1576, 1929
Cfr13I GGNCC 6 cut(s) 28, 64, 407, 1187, 2588, 2589
Cfr9I CCCGGG 1 cut(s) 633
ClaI ATCGAT 1 cut(s) 1288
CseI GACGC 1 cut(s) 1742
CsiI ACCWGGT 1 cut(s) 3015
Csp6I GTAC 7 cut(s) 911, 2234, 2774, 3233, 3515, 3611, 3778
CviQI GTAC 7 cut(s) 911, 2234, 2774, 3233, 3515, 3611, 3778
DraI TTTAAA 2 cut(s) 580, 1120
DraIII CACNNNGTG 1 cut(s) 3388
DrdI GACNNNNNNGTC 1 cut(s) 990
DseDI GACNNNNNNGTC 1 cut(s) 990
EaeI YGGCCR 1 cut(s) 51
Eam1104I CTCTTC 4 cut(s) 649, 2225, 2490, 2937
EarI CTCTTC 4 cut(s) 649, 2225, 2490, 2937
EciI GGCGGA 2 cut(s) 20, 1846
Ecl136II GAGCTC 1 cut(s) 3541
Eco130I CCWWGG 3 cut(s) 1140, 1152, 3001
Eco24I GRGCYC 2 cut(s) 2592, 3543
Eco31I GGTCTC 2 cut(s) 257, 2315
Eco47I GGWCC 3 cut(s) 28, 64, 1187
Eco47III AGCGCT 1 cut(s) 2648
Eco53kI GAGCTC 1 cut(s) 3541
Eco72I CACGTG 1 cut(s) 1232
Eco81I CCTNAGG 3 cut(s) 903, 1731, 3141
Eco88I CYCGRG 2 cut(s) 633, 3722
EcoICRI GAGCTC 1 cut(s) 3541
EcoNI CCTNNNNNAGG 1 cut(s) 414
EcoO109I RGGNCCY 3 cut(s) 407, 2588, 2589
EcoRII CCWGG 3 cut(s) 24, 2500, 3015
EcoT14I CCWWGG 3 cut(s) 1140, 1152, 3001
EcoT38I GRGCYC 2 cut(s) 2592, 3543
ErhI CCWWGG 3 cut(s) 1140, 1152, 3001
Esp3I CGTCTC 1 cut(s) 596
FaqI GGGAC 5 cut(s) 50, 650, 1359, 1579, 3514
FauI CCCGC 2 cut(s) 130, 3705
FauNDI CATATG 1 cut(s) 3212
FbaI TGATCA 2 cut(s) 2002, 2596
FblI GTMKAC 4 cut(s) 72, 2026, 2126, 2245
FokI GGATG 3 cut(s) 2867, 3120, 3798
FriOI GRGCYC 2 cut(s) 2592, 3543
GlaI GCGC 4 cut(s) 36, 475, 1615, 2648
GsuI CTGGAG 2 cut(s) 2339, 3411
HaeII RGCGCY 1 cut(s) 2650
HaeIII GGCC 5 cut(s) 12, 53, 90, 409, 2590
HapII CCGG 7 cut(s) 50, 68, 209, 634, 1577, 1930, 3260
HgaI GACGC 1 cut(s) 1742
HhaI GCGC 4 cut(s) 37, 476, 1616, 2649
Hin6I GCGC 4 cut(s) 35, 474, 1614, 2647
HinP1I GCGC 4 cut(s) 35, 474, 1614, 2647
HincII GTYRAC 2 cut(s) 73, 2127
HindII GTYRAC 2 cut(s) 73, 2127
HindIII AAGCTT 5 cut(s) 1272, 2075, 2276, 3025, 3048
HpaII CCGG 7 cut(s) 50, 68, 209, 634, 1577, 1930, 3260
HphI GGTGA 8 cut(s) 137, 173, 224, 619, 1733, 3754, 3783, 3789
Hpy166II GTNNAC 6 cut(s) 73, 2027, 2127, 2246, 2285, 3611
Hpy8I GTNNAC 6 cut(s) 73, 2027, 2127, 2246, 2285, 3611
Hpy99I CGWCG 4 cut(s) 77, 89, 2128, 2131
HpyCH4III ACNGT 8 cut(s) 58, 607, 1601, 1721, 2765, 3608, 3789, 3795
HpyCH4IV ACGT 6 cut(s) 1231, 2123, 2248, 2370, 2869, 3384
HpySE526I ACGT 6 cut(s) 1231, 2123, 2248, 2370, 2869, 3384
HspAI GCGC 4 cut(s) 35, 474, 1614, 2647
Kpn2I TCCGGA 1 cut(s) 3259
KpnI GGTACC 1 cut(s) 3781
KroI GCCGGC 1 cut(s) 1929
KroNI GCCGGC 1 cut(s) 1931
Ksp22I TGATCA 2 cut(s) 2002, 2596
LguI GCTCTTC 1 cut(s) 649
LmnI GCTCC 5 cut(s) 102, 482, 823, 3430, 3538
LweI GCATC 4 cut(s) 2467, 2650, 2889, 3499
MabI ACCWGGT 1 cut(s) 3015
MaeII ACGT 6 cut(s) 1231, 2123, 2248, 2370, 2869, 3384
MfeI CAATTG 2 cut(s) 1690, 2640
MflI RGATCY 5 cut(s) 21, 1442, 2206, 3587, 3716
MhlI GDGCHC 3 cut(s) 1393, 2592, 3543
MluI ACGCGT 1 cut(s) 2303
MlyI GAGTC 5 cut(s) 190, 1502, 2809, 3284, 3734
MmeI TCCRAC 4 cut(s) 1336, 1655, 2446, 3051
MroI TCCGGA 1 cut(s) 3259
MroNI GCCGGC 1 cut(s) 1929
MroXI GAANNNNTTC 2 cut(s) 3169, 3471
MslI CAYNNNNRTG 6 cut(s) 718, 1014, 1609, 1947, 2475, 2658
MspA1I CMGCKG 1 cut(s) 3579
MspCI CTTAAG 1 cut(s) 3674
MspI CCGG 7 cut(s) 50, 68, 209, 634, 1577, 1930, 3260
MspR9I CCNGG 7 cut(s) 26, 68, 210, 634, 635, 2502, 3017
MunI CAATTG 2 cut(s) 1690, 2640
Mva1269I GAATGC 1 cut(s) 934
MvaI CCWGG 3 cut(s) 26, 2502, 3017
MvnI CGCG 3 cut(s) 474, 2305, 3712
NaeI GCCGGC 1 cut(s) 1931
NciI CCSGG 4 cut(s) 68, 210, 634, 635
NcoI CCATGG 1 cut(s) 1140
NdeI CATATG 1 cut(s) 3212
NgoMIV GCCGGC 1 cut(s) 1929
NlaIV GGNNCC 7 cut(s) 66, 408, 2590, 2591, 3294, 3426, 3779
NmeAIII GCCGAG 1 cut(s) 2854
NmuCI GTSAC 6 cut(s) 1694, 1721, 3019, 3276, 3789, 3795
NspI RCATGY 7 cut(s) 1376, 1614, 1799, 1877, 1946, 3362, 3374
PaeI GCATGC 2 cut(s) 1614, 3362
PagI TCATGA 3 cut(s) 744, 1999, 2607
PciI ACATGT 2 cut(s) 1873, 1942
PciSI GCTCTTC 1 cut(s) 649
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 2819
PctI GAATGC 1 cut(s) 934
PdiI GCCGGC 1 cut(s) 1931
PdmI GAANNNNTTC 2 cut(s) 3169, 3471
PleI GAGTC 5 cut(s) 189, 1501, 2808, 3283, 3733
PmaCI CACGTG 1 cut(s) 1232
PmlI CACGTG 1 cut(s) 1232
PpsI GAGTC 5 cut(s) 189, 1501, 2808, 3283, 3733
Ppu21I YACGTR 1 cut(s) 1232
PscI ACATGT 2 cut(s) 1873, 1942
PshAI GACNNNNGTC 1 cut(s) 3506
Psp124BI GAGCTC 1 cut(s) 3543
Psp6I CCWGG 3 cut(s) 24, 2500, 3015
PspCI CACGTG 1 cut(s) 1232
PspGI CCWGG 3 cut(s) 24, 2500, 3015
PspN4I GGNNCC 7 cut(s) 66, 408, 2590, 2591, 3294, 3426, 3779
PspOMI GGGCCC 1 cut(s) 2588
PspPI GGNCC 6 cut(s) 28, 64, 407, 1187, 2588, 2589
PstI CTGCAG 1 cut(s) 180
PsuI RGATCY 5 cut(s) 21, 1442, 2206, 3587, 3716
PvuII CAGCTG 1 cut(s) 3579
RsaI GTAC 7 cut(s) 912, 2235, 2775, 3234, 3516, 3612, 3779
RsaNI GTAC 7 cut(s) 911, 2234, 2774, 3233, 3515, 3611, 3778
RseI CAYNNNNRTG 6 cut(s) 718, 1014, 1609, 1947, 2475, 2658
SacI GAGCTC 1 cut(s) 3543
SalI GTCGAC 2 cut(s) 71, 2125
SapI GCTCTTC 1 cut(s) 649
Sau96I GGNCC 6 cut(s) 28, 64, 407, 1187, 2588, 2589
SchI GAGTC 5 cut(s) 190, 1502, 2809, 3284, 3734
ScrFI CCNGG 7 cut(s) 26, 68, 210, 634, 635, 2502, 3017
SduI GDGCHC 3 cut(s) 1393, 2592, 3543
SexAI ACCWGGT 1 cut(s) 3015
SfaNI GCATC 4 cut(s) 2467, 2650, 2889, 3499
SfcI CTRYAG 2 cut(s) 176, 1101
SgrDI CGTCGACG 1 cut(s) 2125
SinI GGWCC 3 cut(s) 28, 64, 1187
SmaI CCCGGG 1 cut(s) 635
SmiMI CAYNNNNRTG 6 cut(s) 718, 1014, 1609, 1947, 2475, 2658
SmlI CTYRAG 4 cut(s) 92, 104, 3583, 3674
SmoI CTYRAG 4 cut(s) 92, 104, 3583, 3674
SphI GCATGC 2 cut(s) 1614, 3362
SspI AATATT 2 cut(s) 1914, 2143
SstI GAGCTC 1 cut(s) 3543
StyD4I CCNGG 7 cut(s) 24, 66, 208, 632, 633, 2500, 3015
StyI CCWWGG 3 cut(s) 1140, 1152, 3001
TaaI ACNGT 8 cut(s) 58, 607, 1601, 1721, 2765, 3608, 3789, 3795
TaiI ACGT 6 cut(s) 1234, 2126, 2251, 2373, 2872, 3387
TaqII GACCGA 2 cut(s) 52, 1272
TatI WGTACW 3 cut(s) 910, 3514, 3610
TauI GCSGC 3 cut(s) 464, 670, 1403
TscAI CASTG 7 cut(s) 691, 1179, 1465, 2574, 2770, 3291, 3327
TseFI GTSAC 6 cut(s) 1694, 1721, 3019, 3276, 3789, 3795
Tsp45I GTSAC 6 cut(s) 1694, 1721, 3019, 3276, 3789, 3795
TspGWI ACGGA 6 cut(s) 20, 567, 2052, 3335, 3412, 3768
TspMI CCCGGG 1 cut(s) 633
TspRI CASTG 7 cut(s) 691, 1179, 1465, 2574, 2770, 3291, 3327
Vha464I CTTAAG 1 cut(s) 3674
VpaK11BI GGWCC 3 cut(s) 28, 64, 1187
XagI CCTNNNNNAGG 1 cut(s) 414
XapI RAATTY 1 cut(s) 574
XbaI TCTAGA 1 cut(s) 2707
XceI RCATGY 7 cut(s) 1376, 1614, 1799, 1877, 1946, 3362, 3374
XcmI CCANNNNNNNNNTGG 2 cut(s) 2143, 3303
XmaI CCCGGG 1 cut(s) 633
XmiI GTMKAC 4 cut(s) 72, 2026, 2126, 2245
XmnI GAANNNNTTC 2 cut(s) 3169, 3471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.