Rh7CG062000

ATP-dependent helicase hrq1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0006845
Physical Location & Seq
Reverse (-)
4498789 .. 4507139
8351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG062000.1

Sequence Viewer

Length: 3312 bp
ATGTCCAAGTTTGCGAACTCCGCATGGTCTGATATGATGCAGGACCTGTCATATTTGCGTGATGGTTCGAGTGATGGAACCCCGAGTGTGTCTAGTATAGGGAGTTTTAGTGTTGGAGATGGAAGTGGAGATTGCTCTCGTGAAGTGAAGCGGAAAAGGGGGTTTGGTTGTGATGAGATGATATTGGATATGTTGTGGGCTAGTAGAAGCAAGAGTGTTCTTGATGAACAGAATTCTAGAAGGTTTGTTGAGGTTTTGGAGTCGGTGAATTGTTTATCGGATCCATATTCTGGGGACTGCATGTTGTGGAGAAGAAAAGCTAGCTTACAAGGCTTTGGCTTGGGGCTGCCTAACGGCAAGGGTAATTCATGCTTGTGTCCAGAGTGGTTGAAGAAAATCATGAAGGCGTTTGCTTTCTTAAACATTTTTTCTGCACTTGTTCAACTTCAGCAGGAGAGGACAACCTCAACTCTATTGGAGCAAGCACTGGAACAGCTTGCGACATTTGGAGTTAAACTTGATATGCAGGACATGGAGCATCTTTCGGTTATTTCTCCCAAGGTAGTTCTTTTTGTGAAGAAGAATGCAGAGGAGACAAGTTCTGGTAATGCACTTGTTATTATTGAATGCTCGGCAGAACAGAACCATGGAGACAGAAATAATCATGAATCTGCTTGCAAACAGATGGATGTATCAAAGATTGTCAATGAATTGAAGATGCAGGAAAGTTCTTTCAAAAGTAATTTGTGGGAGGCTGTTGAGAGGTTAATGTTTATCAAAAGAAATGAGATATCCTCCTTGGAAGACTTTCTCATCTATGTGAAGGATTGTGGTGCTGCTGCAAGAGGAAATAAAGTGAATCAAGCAACGAAAAGCCACACAGCTTCAACTACTGATTCAGACAAAAAGCACTCTGCAGCTTCAACCTCTCGTTCACACAAAAAGCGAGCACAATGTCATAGAACAGGTCCATTGCTACCAGTTGAGATGGTTGAACATCTTAGAAAGAGAATTGGATCAGCAACGAAGATAGTGCATGTCGAAGACATTGGTGCTCGAAATGCCGTTCATGTGGAAGTTCCACAGGAACTATCAGAAAATCTGAGATCTGTACTTCATTCTATTGGAATCACTCAGTTATATAGCCATCAGGCAGAGTCGATACAAGCCTCACTAACTGGCAAGAATGTTGTTGTGGCTACAATGACATCCAGTGGGAAATCTCTTTGCTACAATCTACCGGTCATTGAAGCCCTGTCTCAGAGTTCCTCATCATGTGCTCTATACTTATTTCCAACGAAGGCTTTAGCTCAAGATCAACTAAGAGCTTTCTTAGCTATGACCAAAGGATTCAGTGGTAGCTTAAATGTCGGTATATATGATGGTGACACTAATCAGCAGGAAAGGACACGACTGCGTCAAAATGCTAGACTGCTAATCACAAATCCAGATATGTTGCATGTATCAATCCTGCCACACCACCGAAAATTTGGCCGGATTTTGGCAAATCTTAGGTATGTAGTGATTGATGAGGCCCATGTTTATAAGGGAGCATTTGGTTGTCACACTGCTCTTATACTTAGGCGACTTCGCCGGCTCTGTTCACATGTGTATGGAAGTGATCCTTCTTTTGTGTTTTCTACTGCGACTTCGGCAAATCCTCGTGAGCATTGCATGGAACTTGCGAATTTACCAACTCTAGAGTTGATTCAGAATGATGGAAGCCCTGCTGCTAGAAAGCTTTTCATCCTTTGGAATTCTGAGGACAGCGCAATTGAGGATATATCATTCCTCTTTGCTGAAATGGTCCAGCATGGACTACGGTGCATTGCATTTTGCAAAACACGCAAGCTTTGTGAACTTGTTTTTTGCCATACGCGTGAAATTCTTCAGGAGACAGCACCCCATCTGGCAGAGTCTATATGTTCTTATCGTGCTGGTTACATTGCTGAGGATAGGAGAAGAATAGAGAGTGATTTATTTAACGGGAAACTTTCTGGTATTGCTGCAACAAATGCCCTTGAATTGGGTATTGATGTCGGACACATTGATGTAACTCTTCATTTGGGTTTTCCCGGAAGTATTGCTAGCTTGTGGCAACAAGCTGGCAGAGCCGGAAGGAGAGAGAGACCTTCTCTTGCTGTATACGTTGCATTCGAAGGGCCCCTTGACCAATATTTTATGAAACATCCAAATAAACTTTTTGGAAGCCCAATTGAGTGCTGTCATGTTGATGCTAAGAACCAACAGGTTCTTGAACAGCATTTGGCTTGTGCTGCTCTTGAACACCCACTGAGCTTGTTTTATGATGGGAAGTTTTTCGGTTCTGGCTTAAACAGCGCCATAATGTCTCTTAAAAGTAGAGGATATCTGAGTTATGATCCATCATGTGATTCTTCTGCTAAAATCTGGAACTATATTGGGTATCAGAAAATGCCTTCACATTCAGTTAGTATTCGATCAATTGAAAGAGAGAGATACAAAGTTATGGACCATCAAGAGAAAGAAATCGAAGAGATTGAGGAAAGCAAGGCTTTCTTTCAGGTATATGAAGGTGCTGTTTATTTGAACCAAGGAAAGACATACCTGGTCACGAGCTTAGATTTATCTAGCAAGATTGCTTGGTGCCAAAAAGCTGATGTAAATTATTATACGAAACCCCGTGATCGCACAGATATTCATGTAGTTGGTGGTAATGTGGCTTATCCAGCTCCAGCTAAGCTTTCGAACCAGTTCTCCAGAACAACTGCCCGAGCAGATCCTTGCAGAGTAACAACTACATGGTTTGGATTCTATCGTGTACACAAAGGAAGCAACATGATATTTGATAAAGTTAACCTTGCACTTCCTAAATATACGTATACGTCACAGGCAGTTTGGGTTCCGGTTCCACAATCAATAATTAAAGCAGTGGAGAGTAAACAACTTGAATACCGTGCAGGGCTGCATGCTGCATCACATGCTGTTCTGCACGTTGTGCCTCTACGAATCATTTGTAACTTATCTGACTTGGCTCCAGAGTGTGCGGACCCTCATGACGGCCGCTATTTTCCGGAGAGGATTCTATTATATGATCAGCATCCTGGAGGGACTGGTGTCTGTGTACAGGTTCATCCATTTTTTATGGAGCTCTTGGCTACTGCTCTGGAACTCCTAACATCTTGCAACTGCAGAGAAGATGATGGTTGCCCTAATTGCATTCAGAGTTTTTGTTGCAAGTCATATAATGAGGTTTTACACAAGGATGCAGCAATTATGATCATTAAGGGTGTTCTGGATGCGAATATATCTTCTGACTCTTCTGGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DUF1998 Helicase_C

Protein Analysis

1103

Amino Acids

122.79

Weight (kDa)

7.52

Isoelectric Point (pI)

47.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 379 - 516 4.2e-07 Type III restriction enzyme, res subunit
DEAD PF00270 382 - 552 9.2e-31 DEAD/DEAH box helicase
Helicase_C PF00271 602 - 708 1.5e-12 Helicase conserved C-terminal domain
WHD_HRQ1 PF22982 744 - 822 2.3e-16 ATP-dependent helicase HRQ1, winged helix domain
MZB PF09369 985 - 1067 5.9e-18 MrfA Zn-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1545
AccB1I GGYRCC 1 cut(s) 2625
AccB7I CCANNNNNTGG 1 cut(s) 290
AccI GTMKAC 2 cut(s) 2145, 2861
AccII CGCG 1 cut(s) 1879
AccIII TCCGGA 1 cut(s) 3052
AciI CCGC 4 cut(s) 21, 151, 3026, 3043
AclWI GGATC 6 cut(s) 275, 288, 1024, 1616, 2375, 2753
AcoI YGGCCR 2 cut(s) 1492, 3040
AcsI RAATTY 5 cut(s) 232, 1487, 1687, 1756, 1884
AcuI CTGAAG 2 cut(s) 431, 1874
AdeI CACNNNGTG 1 cut(s) 2977
AfaI GTAC 3 cut(s) 1113, 2802, 3105
AfiI CCNNNNNNNGG 4 cut(s) 290, 1501, 2026, 3038
AflIII ACRYGT 2 cut(s) 1606, 1877
AgeI ACCGGT 1 cut(s) 1240
AjnI CCWGG 2 cut(s) 2586, 3082
AloI GAACNNNNNNTCC 2 cut(s) 2720, 2752
Alw21I GWGCWC 4 cut(s) 952, 1057, 1282, 3132
Alw26I GTCTC 6 cut(s) 587, 645, 1263, 1889, 2122, 2355
AlwI GGATC 6 cut(s) 275, 288, 1024, 1616, 2375, 2753
AlwNI CAGNNNCTG 1 cut(s) 46
Ama87I CYCGRG 2 cut(s) 82, 2751
Aor13HI TCCGGA 1 cut(s) 3052
AoxI GGCC 4 cut(s) 1492, 1533, 2162, 3040
ApaI GGGCCC 1 cut(s) 2166
ApoI RAATTY 5 cut(s) 232, 1487, 1687, 1756, 1884
AsiGI ACCGGT 1 cut(s) 1240
Asp700I GAANNNNTTC 4 cut(s) 807, 1887, 2318, 2732
AspLEI GCGC 2 cut(s) 1772, 2342
AspS9I GGNCC 8 cut(s) 43, 968, 1534, 1807, 2162, 2163, 2491, 3028
AsuC2I CCSGG 1 cut(s) 2076
AsuHPI GGTGA 2 cut(s) 277, 1397
AsuII TTCGAA 2 cut(s) 2157, 2726
AsuNHI GCTAGC 2 cut(s) 320, 2087
AvaI CYCGRG 2 cut(s) 82, 2751
AvaII GGWCC 5 cut(s) 43, 968, 1807, 2491, 3028
BaeGI GKGCMC 1 cut(s) 2166
BamHI GGATCC 1 cut(s) 280
BanI GGYRCC 1 cut(s) 2625
BanII GRGCYC 2 cut(s) 2166, 3132
BauI CACGAG 3 cut(s) 138, 1662, 2593
BbsI GAAGAC 2 cut(s) 810, 1050
Bbv12I GWGCWC 4 cut(s) 952, 1057, 1282, 3132
BbvCI CCTCAGC 1 cut(s) 1950
BceAI ACGGC 3 cut(s) 370, 1049, 3055
BcgI CGANNNNNNTGC 2 cut(s) 1015, 1049
BciT130I CCWGG 2 cut(s) 2588, 3084
BclI TGATCA 2 cut(s) 3073, 3258
BcnI CCSGG 1 cut(s) 2076
BcoDI GTCTC 6 cut(s) 587, 645, 1263, 1889, 2122, 2355
BfaI CTAG 9 cut(s) 93, 201, 237, 321, 1428, 1700, 1734, 2088, 2610
BfmI CTRYAG 2 cut(s) 915, 3169
BfoI RGCGCY 1 cut(s) 2343
BglII AGATCT 1 cut(s) 1106
BlpI GCTNAGC 1 cut(s) 2718
Bme1390I CCNGG 3 cut(s) 2076, 2588, 3084
Bme18I GGWCC 5 cut(s) 43, 968, 1807, 2491, 3028
BmeT110I CYCGRG 2 cut(s) 82, 2751
BmgT120I GGNCC 8 cut(s) 43, 968, 1534, 1807, 2162, 2163, 2491, 3028
BmiI GGNNCC 9 cut(s) 79, 282, 2164, 2165, 2627, 2883, 2889, 3015, 3030
BmrFI CCNGG 3 cut(s) 2076, 2588, 3084
BmsI GCATC 8 cut(s) 27, 547, 708, 2224, 2963, 3088, 3235, 3268
BmtI GCTAGC 2 cut(s) 324, 2091
BoxI GACNNNNGTC 1 cut(s) 3095
BpiI GAAGAC 2 cut(s) 810, 1050
BplI GAGNNNNNCTC 4 cut(s) 779, 811, 2119, 2151
BpmI CTGGAG 4 cut(s) 2697, 2722, 3000, 3105
Bpu10I CCTNAGC 1 cut(s) 1950
Bpu1102I GCTNAGC 1 cut(s) 2718
Bpu14I TTCGAA 2 cut(s) 2157, 2726
BpuEI CTTGAG 1 cut(s) 1296
BpuMI CCSGG 1 cut(s) 2076
BsaAI YACGTR 1 cut(s) 2859
BsaBI GATNNNNATC 1 cut(s) 3078
BsaI GGTCTC 1 cut(s) 2122
BsaJI CCNNGG 4 cut(s) 558, 646, 798, 2572
BsaWI WCCGGW 3 cut(s) 1240, 2884, 3052
BsaXI ACNNNNNCTCC 4 cut(s) 94, 124, 2720, 2750
Bsc4I CCNNNNNNNGG 4 cut(s) 290, 1501, 2026, 3038
Bse118I RCCGGY 2 cut(s) 1240, 1593
Bse1I ACTGG 6 cut(s) 492, 980, 1183, 1212, 2731, 3097
Bse3DI GCAATG 4 cut(s) 971, 1669, 1827, 1944
Bse8I GATNNNNATC 1 cut(s) 3078
BseAI TCCGGA 1 cut(s) 3052
BseBI CCWGG 2 cut(s) 2588, 3084
BseDI CCNNGG 4 cut(s) 558, 646, 798, 2572
BseGI GGATG 8 cut(s) 694, 1208, 1746, 2188, 3079, 3112, 3250, 3283
BseJI GATNNNNATC 1 cut(s) 3078
BseLI CCNNNNNNNGG 4 cut(s) 290, 1501, 2026, 3038
BseMI GCAATG 4 cut(s) 971, 1669, 1827, 1944
BseMII CTCAG 7 cut(s) 1094, 1148, 1274, 1752, 1941, 2285, 2363
BseNI ACTGG 6 cut(s) 492, 980, 1183, 1212, 2731, 3097
BseRI GAGGAG 1 cut(s) 605
BseSI GKGCMC 1 cut(s) 2166
BseX3I CGGCCG 1 cut(s) 3040
BsgI GTGCAG 3 cut(s) 417, 2954, 2958
Bsh1236I CGCG 1 cut(s) 1879
Bsh1285I CGRYCG 1 cut(s) 3043
BshFI GGCC 4 cut(s) 1494, 1535, 2164, 3042
BshNI GGYRCC 1 cut(s) 2625
BshTI ACCGGT 1 cut(s) 1240
BsiEI CGRYCG 1 cut(s) 3043
BsiHKAI GWGCWC 4 cut(s) 952, 1057, 1282, 3132
BsiHKCI CYCGRG 2 cut(s) 82, 2751
BsiSI CCGG 7 cut(s) 1241, 1495, 1594, 2076, 2115, 2885, 3053
BslFI GGGAC 2 cut(s) 308, 3103
BslI CCNNNNNNNGG 4 cut(s) 290, 1501, 2026, 3038
BsmAI GTCTC 6 cut(s) 587, 645, 1263, 1889, 2122, 2355
BsmFI GGGAC 2 cut(s) 308, 3103
BsmI GAATGC 4 cut(s) 589, 632, 2153, 3198
BsnI GGCC 4 cut(s) 1494, 1535, 2164, 3042
Bso31I GGTCTC 1 cut(s) 2122
BsoBI CYCGRG 2 cut(s) 82, 2751
Bsp119I TTCGAA 2 cut(s) 2157, 2726
Bsp120I GGGCCC 1 cut(s) 2162
Bsp1286I GDGCHC 5 cut(s) 952, 1057, 1282, 2166, 3132
Bsp13I TCCGGA 1 cut(s) 3052
Bsp1407I TGTACA 2 cut(s) 2800, 3103
Bsp1720I GCTNAGC 1 cut(s) 2718
Bsp19I CCATGG 1 cut(s) 646
BspACI CCGC 4 cut(s) 21, 151, 3026, 3043
BspANI GGCC 4 cut(s) 1494, 1535, 2164, 3042
BspCNI CTCAG 7 cut(s) 1095, 1147, 1273, 1753, 1942, 2286, 2364
BspEI TCCGGA 1 cut(s) 3052
BspFNI CGCG 1 cut(s) 1879
BspHI TCATGA 3 cut(s) 399, 664, 3034
BspLI GGNNCC 9 cut(s) 79, 282, 2164, 2165, 2627, 2883, 2889, 3015, 3030
BspMAI CTGCAG 2 cut(s) 919, 3173
BspOI GCTAGC 2 cut(s) 324, 2091
BspPI GGATC 6 cut(s) 275, 288, 1024, 1616, 2375, 2753
BspT104I TTCGAA 2 cut(s) 2157, 2726
BspT107I GGYRCC 1 cut(s) 2625
BspTNI GGTCTC 1 cut(s) 2122
BsrDI GCAATG 4 cut(s) 971, 1669, 1827, 1944
BsrFI RCCGGY 2 cut(s) 1240, 1593
BsrGI TGTACA 2 cut(s) 2800, 3103
BsrI ACTGG 6 cut(s) 492, 980, 1183, 1212, 2731, 3097
BssAI RCCGGY 2 cut(s) 1240, 1593
BssECI CCNNGG 4 cut(s) 558, 646, 798, 2572
BssNAI GTATAC 2 cut(s) 2146, 2862
BssSI CACGAG 3 cut(s) 138, 1662, 2593
BssT1I CCWWGG 4 cut(s) 558, 646, 798, 2572
Bst1107I GTATAC 2 cut(s) 2146, 2862
Bst2BI CACGAG 3 cut(s) 138, 1662, 2593
Bst2UI CCWGG 2 cut(s) 2588, 3084
Bst4CI ACNGT 2 cut(s) 1824, 2936
Bst6I CTCTTC 3 cut(s) 2064, 2508, 3304
BstAPI GCANNNNNTGC 3 cut(s) 2015, 2960, 2977
BstAUI TGTACA 2 cut(s) 2800, 3103
BstBAI YACGTR 1 cut(s) 2859
BstBI TTCGAA 2 cut(s) 2157, 2726
BstDSI CCRYGG 1 cut(s) 646
BstF5I GGATG 8 cut(s) 694, 1208, 1746, 2188, 3079, 3112, 3250, 3283
BstFNI CGCG 1 cut(s) 1879
BstH2I RGCGCY 1 cut(s) 2343
BstHHI GCGC 2 cut(s) 1772, 2342
BstMAI GTCTC 6 cut(s) 587, 645, 1263, 1889, 2122, 2355
BstMCI CGRYCG 1 cut(s) 3043
BstNI CCWGG 2 cut(s) 2588, 3084
BstNSI RCATGY 6 cut(s) 304, 1040, 1463, 1610, 2951, 2963
BstPAI GACNNNNGTC 1 cut(s) 3095
BstSCI CCNGG 3 cut(s) 2074, 2586, 3082
BstSFI CTRYAG 2 cut(s) 915, 3169
BstSLI GKGCMC 1 cut(s) 2166
BstSNI TACGTA 1 cut(s) 2859
BstUI CGCG 1 cut(s) 1879
BstV2I GAAGAC 2 cut(s) 810, 1050
BstX2I RGATCY 4 cut(s) 280, 1106, 2758, 3305
BstYI RGATCY 4 cut(s) 280, 1106, 2758, 3305
BstZ17I GTATAC 2 cut(s) 2146, 2862
BstZI CGGCCG 1 cut(s) 3040
BsuRI GGCC 4 cut(s) 1494, 1535, 2164, 3042
BtgI CCRYGG 1 cut(s) 646
BtsCI GGATG 8 cut(s) 694, 1208, 1746, 2188, 3079, 3112, 3250, 3283
BtsI GCAGTG 2 cut(s) 1566, 2916
BtsIMutI CAGTG 6 cut(s) 485, 1219, 1360, 1566, 2291, 2916
CaiI CAGNNNCTG 1 cut(s) 46
CciI TCATGA 3 cut(s) 399, 664, 3034
CfoI GCGC 2 cut(s) 1772, 2342
Cfr10I RCCGGY 2 cut(s) 1240, 1593
Cfr13I GGNCC 8 cut(s) 43, 968, 1534, 1807, 2162, 2163, 2491, 3028
CseI GACGC 1 cut(s) 1406
CsiI ACCWGGT 1 cut(s) 2586
Csp6I GTAC 3 cut(s) 1112, 2801, 3104
CspAI ACCGGT 1 cut(s) 1240
CspCI CAANNNNNGTGG 2 cut(s) 2280, 2315
CviQI GTAC 3 cut(s) 1112, 2801, 3104
DraIII CACNNNGTG 1 cut(s) 2977
EaeI YGGCCR 2 cut(s) 1492, 3040
EagI CGGCCG 1 cut(s) 3040
Eam1104I CTCTTC 3 cut(s) 2064, 2508, 3304
EarI CTCTTC 3 cut(s) 2064, 2508, 3304
Ecl136II GAGCTC 1 cut(s) 3130
EclXI CGGCCG 1 cut(s) 3040
Eco105I TACGTA 1 cut(s) 2859
Eco130I CCWWGG 4 cut(s) 558, 646, 798, 2572
Eco24I GRGCYC 2 cut(s) 2166, 3132
Eco31I GGTCTC 1 cut(s) 2122
Eco32I GATATC 2 cut(s) 792, 2369
Eco47I GGWCC 5 cut(s) 43, 968, 1807, 2491, 3028
Eco52I CGGCCG 1 cut(s) 3040
Eco53kI GAGCTC 1 cut(s) 3130
Eco57I CTGAAG 2 cut(s) 431, 1874
Eco88I CYCGRG 2 cut(s) 82, 2751
EcoICRI GAGCTC 1 cut(s) 3130
EcoO109I RGGNCCY 3 cut(s) 43, 2162, 2163
EcoRI GAATTC 2 cut(s) 232, 1756
EcoRII CCWGG 2 cut(s) 2586, 3082
EcoRV GATATC 2 cut(s) 792, 2369
EcoT14I CCWWGG 4 cut(s) 558, 646, 798, 2572
EcoT38I GRGCYC 2 cut(s) 2166, 3132
ErhI CCWWGG 4 cut(s) 558, 646, 798, 2572
FaqI GGGAC 2 cut(s) 308, 3103
FbaI TGATCA 2 cut(s) 3073, 3258
FblI GTMKAC 2 cut(s) 2145, 2861
FokI GGATG 8 cut(s) 701, 1195, 1733, 2175, 3066, 3099, 3257, 3290
FriOI GRGCYC 2 cut(s) 2166, 3132
FspBI CTAG 9 cut(s) 93, 201, 237, 321, 1428, 1700, 1734, 2088, 2610
GlaI GCGC 2 cut(s) 1771, 2341
GsuI CTGGAG 4 cut(s) 2697, 2722, 3000, 3105
HaeII RGCGCY 1 cut(s) 2343
HaeIII GGCC 4 cut(s) 1494, 1535, 2164, 3042
HapII CCGG 7 cut(s) 1241, 1495, 1594, 2076, 2115, 2885, 3053
HgaI GACGC 1 cut(s) 1406
HhaI GCGC 2 cut(s) 1772, 2342
Hin6I GCGC 2 cut(s) 1770, 2340
HinP1I GCGC 2 cut(s) 1770, 2340
HincII GTYRAC 1 cut(s) 2836
HindII GTYRAC 1 cut(s) 2836
HindIII AAGCTT 3 cut(s) 1739, 1850, 2720
HpaI GTTAAC 1 cut(s) 2836
HpaII CCGG 7 cut(s) 1241, 1495, 1594, 2076, 2115, 2885, 3053
HphI GGTGA 2 cut(s) 277, 1397
HpyCH4III ACNGT 2 cut(s) 1824, 2936
HpyCH4IV ACGT 4 cut(s) 2148, 2858, 2864, 2973
HpySE526I ACGT 4 cut(s) 2148, 2858, 2864, 2973
HspAI GCGC 2 cut(s) 1770, 2340
Kpn2I TCCGGA 1 cut(s) 3052
KroI GCCGGC 1 cut(s) 1593
KroNI GCCGGC 1 cut(s) 1595
Ksp22I TGATCA 2 cut(s) 3073, 3258
KspAI GTTAAC 1 cut(s) 2836
LmnI GCTCC 6 cut(s) 478, 535, 1550, 2716, 3019, 3127
LweI GCATC 8 cut(s) 27, 547, 708, 2224, 2963, 3088, 3235, 3268
MabI ACCWGGT 1 cut(s) 2586
MaeI CTAG 9 cut(s) 93, 201, 237, 321, 1428, 1700, 1734, 2088, 2610
MaeII ACGT 4 cut(s) 2148, 2858, 2864, 2973
MaeIII GTNAC 8 cut(s) 1385, 1562, 1940, 2053, 2590, 2770, 2865, 2996
MfeI CAATTG 3 cut(s) 1773, 2214, 2463
MflI RGATCY 4 cut(s) 280, 1106, 2758, 3305
MhlI GDGCHC 5 cut(s) 952, 1057, 1282, 2166, 3132
MluI ACGCGT 1 cut(s) 1877
MlyI GAGTC 4 cut(s) 269, 1166, 1925, 3290
MmeI TCCRAC 3 cut(s) 94, 1319, 2020
MroI TCCGGA 1 cut(s) 3052
MroNI GCCGGC 1 cut(s) 1593
MroXI GAANNNNTTC 4 cut(s) 807, 1887, 2318, 2732
MslI CAYNNNNRTG 7 cut(s) 373, 818, 1611, 1878, 2049, 2232, 3243
MspI CCGG 7 cut(s) 1241, 1495, 1594, 2076, 2115, 2885, 3053
MspR9I CCNGG 3 cut(s) 2076, 2588, 3084
MunI CAATTG 3 cut(s) 1773, 2214, 2463
Mva1269I GAATGC 4 cut(s) 589, 632, 2153, 3198
MvaI CCWGG 2 cut(s) 2588, 3084
MvnI CGCG 1 cut(s) 1879
NaeI GCCGGC 1 cut(s) 1595
NciI CCSGG 1 cut(s) 2076
NcoI CCATGG 1 cut(s) 646
NgoMIV GCCGGC 1 cut(s) 1593
NheI GCTAGC 2 cut(s) 320, 2087
NlaIV GGNNCC 9 cut(s) 79, 282, 2164, 2165, 2627, 2883, 2889, 3015, 3030
NmeAIII GCCGAG 1 cut(s) 611
NmuCI GTSAC 4 cut(s) 1385, 1562, 2590, 2865
NspI RCATGY 6 cut(s) 304, 1040, 1463, 1610, 2951, 2963
NspV TTCGAA 2 cut(s) 2157, 2726
PaeI GCATGC 1 cut(s) 2951
PagI TCATGA 3 cut(s) 399, 664, 3034
PciI ACATGT 1 cut(s) 1606
PcsI WCGNNNNNNNCGW 1 cut(s) 2154
PctI GAATGC 4 cut(s) 589, 632, 2153, 3198
PdiI GCCGGC 1 cut(s) 1595
PdmI GAANNNNTTC 4 cut(s) 807, 1887, 2318, 2732
PflFI GACNNNGTC 1 cut(s) 1416
PflMI CCANNNNNTGG 1 cut(s) 290
PfoI TCCNGGA 2 cut(s) 2074, 3082
PinAI ACCGGT 1 cut(s) 1240
PleI GAGTC 4 cut(s) 268, 1165, 1924, 3290
PpsI GAGTC 4 cut(s) 268, 1165, 1924, 3290
Ppu21I YACGTR 1 cut(s) 2859
PpuMI RGGWCCY 1 cut(s) 43
PscI ACATGT 1 cut(s) 1606
PshAI GACNNNNGTC 1 cut(s) 3095
PsiI TTATAA 1 cut(s) 1545
Psp124BI GAGCTC 1 cut(s) 3132
Psp5II RGGWCCY 1 cut(s) 43
Psp6I CCWGG 2 cut(s) 2586, 3082
PspGI CCWGG 2 cut(s) 2586, 3082
PspN4I GGNNCC 9 cut(s) 79, 282, 2164, 2165, 2627, 2883, 2889, 3015, 3030
PspOMI GGGCCC 1 cut(s) 2162
PspPI GGNCC 8 cut(s) 43, 968, 1534, 1807, 2162, 2163, 2491, 3028
PspPPI RGGWCCY 1 cut(s) 43
PstI CTGCAG 2 cut(s) 919, 3173
PstNI CAGNNNCTG 1 cut(s) 46
PsuI RGATCY 4 cut(s) 280, 1106, 2758, 3305
PsyI GACNNNGTC 1 cut(s) 1416
RsaI GTAC 3 cut(s) 1113, 2802, 3105
RsaNI GTAC 3 cut(s) 1112, 2801, 3104
RseI CAYNNNNRTG 7 cut(s) 373, 818, 1611, 1878, 2049, 2232, 3243
SacI GAGCTC 1 cut(s) 3132
Sau96I GGNCC 8 cut(s) 43, 968, 1534, 1807, 2162, 2163, 2491, 3028
SchI GAGTC 4 cut(s) 269, 1166, 1925, 3290
ScrFI CCNGG 3 cut(s) 2076, 2588, 3084
SduI GDGCHC 5 cut(s) 952, 1057, 1282, 2166, 3132
SexAI ACCWGGT 1 cut(s) 2586
SfaNI GCATC 8 cut(s) 27, 547, 708, 2224, 2963, 3088, 3235, 3268
SfcI CTRYAG 2 cut(s) 915, 3169
SfuI TTCGAA 2 cut(s) 2157, 2726
SinI GGWCC 5 cut(s) 43, 968, 1807, 2491, 3028
SmiMI CAYNNNNRTG 7 cut(s) 373, 818, 1611, 1878, 2049, 2232, 3243
SmlI CTYRAG 1 cut(s) 1311
SmoI CTYRAG 1 cut(s) 1311
SnaBI TACGTA 1 cut(s) 2859
SphI GCATGC 1 cut(s) 2951
SsiI CCGC 4 cut(s) 21, 151, 3026, 3043
SspI AATATT 1 cut(s) 2177
SspMI CTAG 9 cut(s) 93, 201, 237, 321, 1428, 1700, 1734, 2088, 2610
SstI GAGCTC 1 cut(s) 3132
StyD4I CCNGG 3 cut(s) 2074, 2586, 3082
StyI CCWWGG 4 cut(s) 558, 646, 798, 2572
TaaI ACNGT 2 cut(s) 1824, 2936
TaiI ACGT 4 cut(s) 2151, 2861, 2867, 2976
TaqI TCGA 8 cut(s) 68, 1041, 1057, 1160, 2157, 2458, 2511, 2726
TatI WGTACW 3 cut(s) 1111, 2800, 3103
TauI GCSGC 1 cut(s) 3045
TscAI CASTG 6 cut(s) 492, 1219, 1360, 1573, 2298, 2916
TseFI GTSAC 4 cut(s) 1385, 1562, 2590, 2865
Tsp45I GTSAC 4 cut(s) 1385, 1562, 2590, 2865
TspRI CASTG 6 cut(s) 492, 1219, 1360, 1573, 2298, 2916
Tth111I GACNNNGTC 1 cut(s) 1416
Van91I CCANNNNNTGG 1 cut(s) 290
VpaK11BI GGWCC 5 cut(s) 43, 968, 1807, 2491, 3028
XapI RAATTY 5 cut(s) 232, 1487, 1687, 1756, 1884
XbaI TCTAGA 2 cut(s) 236, 1699
XceI RCATGY 6 cut(s) 304, 1040, 1463, 1610, 2951, 2963
XcmI CCANNNNNNNNNTGG 1 cut(s) 1487
XmiI GTMKAC 2 cut(s) 2145, 2861
XmnI GAANNNNTTC 4 cut(s) 807, 1887, 2318, 2732
XspI CTAG 9 cut(s) 93, 201, 237, 321, 1428, 1700, 1734, 2088, 2610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.