FvH4_5g16180

Pentatricopeptide repeat-containing protein At1g74850

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009497
Physical Location & Seq
Reverse (-)
9228412 .. 9232846
4435 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g16180.t1

Sequence Viewer

Length: 2589 bp
ATGACCCTCTCCCCCACCCTCTCCATCTCCCGCCCTTCCCCTCTCTCCGCCCCAATCCCCAAGCTAAACCCTAAACCCCACCACCTCTCCTTCCTCTCCGGCCACCGCAAATTCATCCACGGCCAGCGGCTATCTTTCTCCGTCAGGGCGAAGCCGAAGGACCTCATCCTCGGGAACCCGTCCGTCACCGTCGAAAAGGGCAAGTACAGCTACGACGTCGAAACCCTAATCAACAAGCTCAGCAGCCTCCCGCCACGTGGCAGCATCGCGCGGTGCCTGGACATCTTCAAGAACAAGCTCTCCCTCAACGACTTCGCCCTGGTGTTCAAGGAGTTCGCGGCGCGTGGCGATTGGCAGAGGTCGCTGCGGCTGTTCAAGTATATGCAGCGCCAGATATGGTGCAAGCCGAGCGAGCACATCTACACCATTATGATCAGTCTGCTCGGCCGCGAAGGTTTGCTTGATAAATGCGCAGAGATTTTCGACGAAATGCCCACCCAGGGTGTCATCAGGAGTGTGTTCAGCTACACTGCTTTGATTAATGCCTACGGGCGTAATGGTCAGTTTGAAATGTCTCTTCAATTGCTTGATAGGATGAAGAAAGATAAGGTTTCCCCGAATATATTGACTTATAATACTGTGCTTAATGCGTGTGCTAGAGGGGGGTTGGATTGGGAGGGGCTGTTAGGATTGTTTGCGGAAATGAGGCATGAGGGGGTTCAGCCGGACCTTGTTACTTATAATACACTGCTTAGTGCTTGCGCCGGTAGAGGTTTAGGTGATGAGGCGGAGATGGTGTTTAGGACTATGAATGAGGGTGGGATAGTTCCAGATATAACCACCTATAGTTATCTTGTAGAAACTTTTGGTAAATTGAATAACCTTGAGAAGGTTTCGGAGTTGCTTAAAGGGATGGAATCTGGGGGGAATTTGCCTGATATTACGTCGTATAATGTGTTGTTGGAGGCGTATGCGCAGTTGGGGTCGATTAAAGAGGCAATGGGTGTGTTTAGGCAGATGCAGGAGGCAGGTTGTATGGCAAATGCGGCTACCTATAGTATTTTGTTGAATTTGTATGGGAGGCTTGGGAGGTATGATGATGTTAGGGAGCTTTTTCTTGAGATGAAAGTGAGCAATGCGGAGCCAGATGCTGCTACGTATAATATTCTCATACAAGTGTTTGGTGAAGGTGGGTATTTCCGAGAGGTGGTGACTTTGTTTCATGATATGGTAGAGGAGAATATTGAGCCGAATATGGAGACGTATGAAGGATTGATATATGCTTGTGGAAAAGGAGGGCTTCATGAGGATGCCAAGAACATTTTGCTTCACATGAATGAGAAGGGAATAGTGCCTAGTTCCAAGGCTTATACAGGAGCTATTGAAGCATATGGGCAAGCAGCACTGTATGATGAAGCTCTTGTTGCCTTTAACACAATGAATGAAGTCGGCAGCAGCCCATCAGTTGAAAGCTTCAACTCGCTTATTCATGCATATGCAAGAGGTGGACTATACAAGGAGACTGAACAAGTGTTGTCGATAATGGGTGAGTTTGGCATTGCAATCAATGCCAGTTCATTCAATGGAATGATTGAAGCTTTTAGGCAGGGAGGTCAGTTTGAAGAAGCTATAAAGACGTATGTTGAGATGGAAAAGAGAAGATGTGATCCTGATGAGTGCACTCTCGAAGCAGTCTTAAGCGTTTATAGTGTTGCAGGTCTTGTTAATGAGTGTGAGGAGCACTTCGAAGAGATTAAAGCTTCAGGGATATTACCCAGCGTCATGTGCTACTGCATGATGCTTGCTGTTTATGCAAAGACTGACAGGTGGGACGATGCCAATAAGTTGCTGAATGAGATGCTCACAAATAGGGTATCAAATATTCATCAAGTGATGGGGCAGATGATAAAGGGAGATTATGATGATGAATCTAACTGGCAGATGGTAGAGTATGTTTTTGACAAACTAAAATCTGAAGGATGTGGGCTGGGAATGAGGTTCTACAACACTCTGATAGAAGCACTTTGGTGGCTGGGTCAGAAACAAAGAGCTGTGCGAGTTCTTAGTGAAGCAACACAACGGGGGCTGTTTCCTGAACTTCTCCGCAAAAATAAACTTGTTTGGTCTATTGATGTGCACAGGATGTGGGAAGGTGGTGCATATGCAGCAATGTCAGTTTGGCTAAATGATATGTATGAGATGTTCTTGAATGGCGAGGATCTTCCCCATGTTGCAACAGTTGTTGTAGTACGGGGGAAGATGGAAAAAAGTTCAACGACACAAGATCTGCCGGTTGCAAAGGCTGCCTACTCATTCCTGCAGGATAACATGTCAGGAGCATTCAATTTCCCAAAATGGAACAACGGTCGAATTCTCTGCCAACGGTCTCAGCTTAAGAAACTTCTGTCAAGCATCGAACCATCTACAGATGGCTCCAGTAGTAAAAGTATATGTATTTTAAGCAACTCCCCCTTTCCTCCTCCAGGCACAAAAATATCACCCACCGATGTTGATAGTGGTCGGTATAATGGTACCAGTTCTGATGCAACAAGTAGGACAAGAACAGAGCTTTTGACCAGCACAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

863

Amino Acids

96.31

Weight (kDa)

5.56

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 112 - 219 6.8e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 136 - 180 3.5e-06 PPR repeat family
PPR_3 PF13812 160 - 218 1.1e-14 Pentatricopeptide repeat domain
PPR_2 PF13041 172 - 220 1.3e-14 PPR repeat family
PPR PF01535 174 - 203 1.2e-07 PPR repeat
PPR_long PF17177 193 - 329 3.4e-11 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 214 - 340 5.3e-09 Fungal tetratrico peptide repeats
PPR_2 PF13041 229 - 255 4.2e-06 PPR repeat family
PPR_3 PF13812 231 - 291 7e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 239 - 270 7.2e-06 PPR repeat
PPR_2 PF13041 242 - 289 2.8e-11 PPR repeat family
PPR_3 PF13812 301 - 360 3.9e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 312 - 357 3.2e-12 PPR repeat family
PPR PF01535 316 - 345 6.2e-07 PPR repeat
TPR_24 PF23276 316 - 408 6.2e-07 Fungal tetratrico peptide repeats
PPR_2 PF13041 355 - 394 5.2e-08 PPR repeat family
PPR_2 PF13041 386 - 431 3.2e-09 PPR repeat family
PPR_3 PF13812 406 - 466 3.5e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 523 - 566 3.3e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 633, 741
AatII GACGTC 1 cut(s) 219
Acc16I TGCGCA 2 cut(s) 472, 975
Acc36I ACCTGC 2 cut(s) 1019, 1706
Acc65I GGTACC 1 cut(s) 2531
AccB1I GGYRCC 2 cut(s) 273, 2531
AccII CGCG 5 cut(s) 269, 271, 338, 343, 450
AclWI GGATC 2 cut(s) 1661, 2226
AcoI YGGCCR 3 cut(s) 100, 121, 445
AcsI RAATTY 4 cut(s) 110, 928, 1069, 2370
AcuI CTGAAG 2 cut(s) 1746, 1995
AcvI CACGTG 1 cut(s) 257
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 3 cut(s) 206, 2250, 2533
AfiI CCNNNNNNNGG 5 cut(s) 257, 500, 889, 1207, 2483
AflII CTTAAG 2 cut(s) 1696, 2393
AflIII ACRYGT 1 cut(s) 2328
AjnI CCWGG 4 cut(s) 276, 318, 498, 2482
AleI CACNNNNGTG 1 cut(s) 2026
AloI GAACNNNNNNTCC 2 cut(s) 284, 316
Alw21I GWGCWC 4 cut(s) 417, 1682, 1743, 2139
Alw26I GTCTC 4 cut(s) 579, 1253, 1514, 2391
Alw44I GTGCAC 2 cut(s) 1678, 2135
AlwI GGATC 2 cut(s) 1661, 2226
AlwNI CAGNNNCTG 1 cut(s) 1151
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 3 cut(s) 100, 121, 445
ApaLI GTGCAC 2 cut(s) 1678, 2135
ApoI RAATTY 4 cut(s) 110, 928, 1069, 2370
AseI ATTAAT 1 cut(s) 540
Asp718I GGTACC 1 cut(s) 2531
AspLEI GCGC 6 cut(s) 271, 343, 390, 473, 764, 976
AspS9I GGNCC 2 cut(s) 160, 727
AsuHPI GGTGA 6 cut(s) 178, 791, 1196, 1222, 1559, 2490
AsuII TTCGAA 1 cut(s) 1746
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 2 cut(s) 160, 727
BaeGI GKGCMC 2 cut(s) 1682, 2139
BanI GGYRCC 2 cut(s) 273, 2531
BbrPI CACGTG 1 cut(s) 257
Bbv12I GWGCWC 4 cut(s) 417, 1682, 1743, 2139
BceAI ACGGC 1 cut(s) 136
BcgI CGANNNNNNTGC 2 cut(s) 2358, 2392
BciT130I CCWGG 4 cut(s) 278, 320, 500, 2484
BclI TGATCA 1 cut(s) 432
BcoDI GTCTC 4 cut(s) 579, 1253, 1514, 2391
BfaI CTAG 2 cut(s) 657, 1356
BfmI CTRYAG 4 cut(s) 844, 1054, 2318, 2424
BfoI RGCGCY 1 cut(s) 391
BfrI CTTAAG 2 cut(s) 1696, 2393
BfuAI ACCTGC 2 cut(s) 1019, 1706
BglII AGATCT 1 cut(s) 2284
BlpI GCTNAGC 1 cut(s) 239
Bme1390I CCNGG 4 cut(s) 278, 320, 500, 2484
Bme18I GGWCC 2 cut(s) 160, 727
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 2 cut(s) 160, 727
BmiI GGNNCC 5 cut(s) 176, 275, 1143, 2434, 2533
BmrFI CCNGG 4 cut(s) 278, 320, 500, 2484
BmsI GCATC 9 cut(s) 273, 1008, 1138, 1300, 1788, 1825, 1848, 2421, 2533
BpmI CTGGAG 2 cut(s) 2419, 2466
Bpu1102I GCTNAGC 1 cut(s) 239
Bpu14I TTCGAA 1 cut(s) 1746
BpuEI CTTGAG 2 cut(s) 905, 1139
BsaAI YACGTR 2 cut(s) 257, 1158
BsaHI GRCGYC 1 cut(s) 216
BsaI GGTCTC 1 cut(s) 2391
BsaJI CCNNGG 6 cut(s) 118, 169, 318, 498, 499, 1362
BsaXI ACNNNNNCTCC 8 cut(s) 71, 101, 284, 314, 668, 698, 782, 812
Bsc4I CCNNNNNNNGG 5 cut(s) 257, 500, 889, 1207, 2483
Bse118I RCCGGY 2 cut(s) 764, 2290
Bse1I ACTGG 4 cut(s) 1572, 1940, 2436, 2535
Bse3DI GCAATG 4 cut(s) 1005, 1141, 1557, 2175
BseBI CCWGG 4 cut(s) 278, 320, 500, 2484
BseDI CCNNGG 6 cut(s) 118, 169, 318, 498, 499, 1362
BseGI GGATG 7 cut(s) 114, 165, 600, 918, 1315, 1985, 2148
BseLI CCNNNNNNNGG 5 cut(s) 257, 500, 889, 1207, 2483
BseMI GCAATG 4 cut(s) 1005, 1141, 1557, 2175
BseMII CTCAG 2 cut(s) 253, 2402
BseNI ACTGG 4 cut(s) 1572, 1940, 2436, 2535
BseRI GAGGAG 3 cut(s) 1250, 1751, 2469
BseSI GKGCMC 2 cut(s) 1682, 2139
BseX3I CGGCCG 1 cut(s) 445
BseYI CCCAGC 3 cut(s) 1775, 1987, 2032
Bsh1236I CGCG 5 cut(s) 269, 271, 338, 343, 450
Bsh1285I CGRYCG 2 cut(s) 448, 2368
BshFI GGCC 3 cut(s) 102, 123, 447
BshNI GGYRCC 2 cut(s) 273, 2531
BsiEI CGRYCG 2 cut(s) 448, 2368
BsiHKAI GWGCWC 4 cut(s) 417, 1682, 1743, 2139
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 4 cut(s) 99, 725, 765, 2291
BslFI GGGAC 1 cut(s) 1844
BslI CCNNNNNNNGG 5 cut(s) 257, 500, 889, 1207, 2483
BsmAI GTCTC 4 cut(s) 579, 1253, 1514, 2391
BsmBI CGTCTC 1 cut(s) 1253
BsmFI GGGAC 1 cut(s) 1844
BsmI GAATGC 1 cut(s) 2339
BsnI GGCC 3 cut(s) 102, 123, 447
Bso31I GGTCTC 1 cut(s) 2391
BsoBI CYCGRG 1 cut(s) 170
Bsp119I TTCGAA 1 cut(s) 1746
Bsp1286I GDGCHC 4 cut(s) 417, 1682, 1743, 2139
Bsp143I GATC 4 cut(s) 432, 1666, 2218, 2284
Bsp1720I GCTNAGC 1 cut(s) 239
BspANI GGCC 3 cut(s) 102, 123, 447
BspCNI CTCAG 2 cut(s) 252, 2401
BspFNI CGCG 5 cut(s) 269, 271, 338, 343, 450
BspHI TCATGA 2 cut(s) 1222, 1303
BspLI GGNNCC 5 cut(s) 176, 275, 1143, 2434, 2533
BspMAI CTGCAG 1 cut(s) 2322
BspMI ACCTGC 2 cut(s) 1019, 1706
BspPI GGATC 2 cut(s) 1661, 2226
BspT104I TTCGAA 1 cut(s) 1746
BspT107I GGYRCC 2 cut(s) 273, 2531
BspTI CTTAAG 2 cut(s) 1696, 2393
BspTNI GGTCTC 1 cut(s) 2391
BsrDI GCAATG 4 cut(s) 1005, 1141, 1557, 2175
BsrFI RCCGGY 2 cut(s) 764, 2290
BsrI ACTGG 4 cut(s) 1572, 1940, 2436, 2535
BssAI RCCGGY 2 cut(s) 764, 2290
BssECI CCNNGG 6 cut(s) 118, 169, 318, 498, 499, 1362
BssMI GATC 4 cut(s) 432, 1666, 2218, 2284
BssNI GRCGYC 1 cut(s) 216
BssT1I CCWWGG 1 cut(s) 1362
Bst2UI CCWGG 4 cut(s) 278, 320, 500, 2484
Bst4CI ACNGT 7 cut(s) 190, 640, 1407, 2239, 2366, 2385, 2584
Bst6I CTCTTC 2 cut(s) 582, 1743
BstACI GRCGYC 1 cut(s) 216
BstAFI CTTAAG 2 cut(s) 1696, 2393
BstAPI GCANNNNNTGC 2 cut(s) 1568, 2303
BstBAI YACGTR 2 cut(s) 257, 1158
BstBI TTCGAA 1 cut(s) 1746
BstC8I GCNNGC 6 cut(s) 125, 404, 413, 760, 1398, 1803
BstDEI CTNAG 4 cut(s) 239, 752, 2063, 2388
BstDSI CCRYGG 1 cut(s) 118
BstENI CCTNNNNNAGG 2 cut(s) 887, 2481
BstF5I GGATG 7 cut(s) 114, 165, 600, 918, 1315, 1985, 2148
BstFNI CGCG 5 cut(s) 269, 271, 338, 343, 450
BstH2I RGCGCY 1 cut(s) 391
BstHHI GCGC 6 cut(s) 271, 343, 390, 473, 764, 976
BstKTI GATC 4 cut(s) 435, 1669, 2221, 2287
BstMAI GTCTC 4 cut(s) 579, 1253, 1514, 2391
BstMBI GATC 4 cut(s) 432, 1666, 2218, 2284
BstMCI CGRYCG 2 cut(s) 448, 2368
BstNI CCWGG 4 cut(s) 278, 320, 500, 2484
BstNSI RCATGY 1 cut(s) 2332
BstSCI CCNGG 4 cut(s) 276, 318, 498, 2482
BstSFI CTRYAG 4 cut(s) 844, 1054, 2318, 2424
BstSLI GKGCMC 2 cut(s) 1682, 2139
BstSNI TACGTA 1 cut(s) 1158
BstUI CGCG 5 cut(s) 269, 271, 338, 343, 450
BstX2I RGATCY 2 cut(s) 2218, 2284
BstYI RGATCY 2 cut(s) 2218, 2284
BstZI CGGCCG 1 cut(s) 445
BsuRI GGCC 3 cut(s) 102, 123, 447
BtgI CCRYGG 1 cut(s) 118
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 7 cut(s) 114, 165, 600, 918, 1315, 1985, 2148
BtsI GCAGTG 2 cut(s) 528, 746
BtsIMutI CAGTG 3 cut(s) 528, 746, 1403
BveI ACCTGC 2 cut(s) 1019, 1706
Cac8I GCNNGC 6 cut(s) 125, 404, 413, 760, 1398, 1803
CaiI CAGNNNCTG 1 cut(s) 1151
CciI TCATGA 2 cut(s) 1222, 1303
CfoI GCGC 6 cut(s) 271, 343, 390, 473, 764, 976
Cfr10I RCCGGY 2 cut(s) 764, 2290
Cfr13I GGNCC 2 cut(s) 160, 727
CseI GACGC 1 cut(s) 1768
Csp6I GTAC 3 cut(s) 205, 2249, 2532
CspCI CAANNNNNGTGG 2 cut(s) 2491, 2526
CviAII CATG 9 cut(s) 710, 1223, 1304, 1333, 1490, 1783, 1795, 2228, 2329
CviQI GTAC 3 cut(s) 205, 2249, 2532
DdeI CTNAG 4 cut(s) 239, 752, 2063, 2388
DpnI GATC 4 cut(s) 434, 1668, 2220, 2286
DpnII GATC 4 cut(s) 432, 1666, 2218, 2284
EaeI YGGCCR 3 cut(s) 100, 121, 445
EagI CGGCCG 1 cut(s) 445
Eam1104I CTCTTC 2 cut(s) 582, 1743
EarI CTCTTC 2 cut(s) 582, 1743
EciI GGCGGA 2 cut(s) 37, 803
EclXI CGGCCG 1 cut(s) 445
Eco105I TACGTA 1 cut(s) 1158
Eco130I CCWWGG 1 cut(s) 1362
Eco31I GGTCTC 1 cut(s) 2391
Eco47I GGWCC 2 cut(s) 160, 727
Eco52I CGGCCG 1 cut(s) 445
Eco57I CTGAAG 2 cut(s) 1746, 1995
Eco72I CACGTG 1 cut(s) 257
Eco88I CYCGRG 1 cut(s) 170
EcoNI CCTNNNNNAGG 2 cut(s) 887, 2481
EcoO109I RGGNCCY 1 cut(s) 160
EcoRI GAATTC 1 cut(s) 2370
EcoRII CCWGG 4 cut(s) 276, 318, 498, 2482
EcoT14I CCWWGG 1 cut(s) 1362
EcoT22I ATGCAT 1 cut(s) 1495
ErhI CCWWGG 1 cut(s) 1362
Esp3I CGTCTC 1 cut(s) 1253
FaeI CATG 9 cut(s) 713, 1226, 1307, 1336, 1493, 1786, 1798, 2231, 2332
FalI AAGNNNNNCTT 4 cut(s) 444, 476, 1284, 1316
FaqI GGGAC 1 cut(s) 1844
FatI CATG 9 cut(s) 709, 1222, 1303, 1332, 1489, 1782, 1794, 2227, 2328
FauI CCCGC 2 cut(s) 38, 258
FauNDI CATATG 3 cut(s) 1390, 1495, 2161
FbaI TGATCA 1 cut(s) 432
FokI GGATG 7 cut(s) 101, 152, 607, 925, 1322, 1992, 2155
FspBI CTAG 2 cut(s) 657, 1356
FspI TGCGCA 2 cut(s) 472, 975
GlaI GCGC 6 cut(s) 270, 342, 389, 472, 763, 975
GsaI CCCAGC 3 cut(s) 1779, 1991, 2036
GsuI CTGGAG 2 cut(s) 2419, 2466
HaeII RGCGCY 1 cut(s) 391
HaeIII GGCC 3 cut(s) 102, 123, 447
HapII CCGG 4 cut(s) 99, 725, 765, 2291
HgaI GACGC 1 cut(s) 1768
HhaI GCGC 6 cut(s) 271, 343, 390, 473, 764, 976
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 9 cut(s) 713, 1226, 1307, 1336, 1493, 1786, 1798, 2231, 2332
Hin6I GCGC 6 cut(s) 269, 341, 388, 471, 762, 974
HinP1I GCGC 6 cut(s) 269, 341, 388, 471, 762, 974
HindIII AAGCTT 3 cut(s) 1471, 1596, 1758
HinfI GANTC 2 cut(s) 917, 1928
HpaII CCGG 4 cut(s) 99, 725, 765, 2291
HphI GGTGA 6 cut(s) 178, 791, 1196, 1222, 1559, 2490
Hpy166II GTNNAC 3 cut(s) 1508, 1680, 2137
Hpy188I TCNGA 6 cut(s) 898, 1202, 1975, 2013, 2040, 2542
Hpy8I GTNNAC 3 cut(s) 1508, 1680, 2137
Hpy99I CGWCG 5 cut(s) 194, 218, 221, 488, 949
HpyCH4III ACNGT 7 cut(s) 190, 640, 1407, 2239, 2366, 2385, 2584
HpyCH4IV ACGT 6 cut(s) 216, 256, 944, 1157, 1262, 1637
HpyF3I CTNAG 4 cut(s) 239, 752, 2063, 2388
HpySE526I ACGT 6 cut(s) 216, 256, 944, 1157, 1262, 1637
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 9 cut(s) 713, 1226, 1307, 1336, 1493, 1786, 1798, 2231, 2332
HspAI GCGC 6 cut(s) 269, 341, 388, 471, 762, 974
KpnI GGTACC 1 cut(s) 2535
Ksp22I TGATCA 1 cut(s) 432
Kzo9I GATC 4 cut(s) 432, 1666, 2218, 2284
LmnI GCTCC 6 cut(s) 1108, 1141, 1376, 1738, 2336, 2438
LweI GCATC 9 cut(s) 273, 1008, 1138, 1300, 1788, 1825, 1848, 2421, 2533
MaeI CTAG 2 cut(s) 657, 1356
MaeII ACGT 6 cut(s) 216, 256, 944, 1157, 1262, 1637
MaeIII GTNAC 3 cut(s) 184, 733, 1210
MalI GATC 4 cut(s) 434, 1668, 2220, 2286
MboI GATC 4 cut(s) 432, 1666, 2218, 2284
MboII GAAGA 8 cut(s) 277, 569, 610, 1634, 1671, 1760, 2213, 2269
MfeI CAATTG 1 cut(s) 581
MflI RGATCY 2 cut(s) 2218, 2284
MhlI GDGCHC 4 cut(s) 417, 1682, 1743, 2139
MluCI AATT 7 cut(s) 110, 581, 872, 928, 1069, 2344, 2370
MmeI TCCRAC 2 cut(s) 648, 942
Mph1103I ATGCAT 1 cut(s) 1495
MslI CAYNNNNRTG 6 cut(s) 428, 1175, 1308, 1335, 1494, 2026
MspA1I CMGCKG 1 cut(s) 127
MspCI CTTAAG 2 cut(s) 1696, 2393
MspI CCGG 4 cut(s) 99, 725, 765, 2291
MspR9I CCNGG 4 cut(s) 278, 320, 500, 2484
MunI CAATTG 1 cut(s) 581
Mva1269I GAATGC 1 cut(s) 2339
MvaI CCWGG 4 cut(s) 278, 320, 500, 2484
MvnI CGCG 5 cut(s) 269, 271, 338, 343, 450
NdeI CATATG 3 cut(s) 1390, 1495, 2161
NdeII GATC 4 cut(s) 432, 1666, 2218, 2284
NlaIII CATG 9 cut(s) 713, 1226, 1307, 1336, 1493, 1786, 1798, 2231, 2332
NlaIV GGNNCC 5 cut(s) 176, 275, 1143, 2434, 2533
NmeAIII GCCGAG 2 cut(s) 423, 432
NmuCI GTSAC 2 cut(s) 184, 1210
NsbI TGCGCA 2 cut(s) 472, 975
NsiI ATGCAT 1 cut(s) 1495
NspI RCATGY 1 cut(s) 2332
NspV TTCGAA 1 cut(s) 1746
OliI CACNNNNGTG 1 cut(s) 2026
PagI TCATGA 2 cut(s) 1222, 1303
PasI CCCWGGG 1 cut(s) 499
PciI ACATGT 1 cut(s) 2328
PctI GAATGC 1 cut(s) 2339
PfeI GAWTC 2 cut(s) 917, 1928
PmaCI CACGTG 1 cut(s) 257
PmlI CACGTG 1 cut(s) 257
Ppu21I YACGTR 2 cut(s) 257, 1158
PpuMI RGGWCCY 1 cut(s) 160
PscI ACATGT 1 cut(s) 2328
PshBI ATTAAT 1 cut(s) 540
PsiI TTATAA 2 cut(s) 633, 741
Psp5II RGGWCCY 1 cut(s) 160
Psp6I CCWGG 4 cut(s) 276, 318, 498, 2482
PspCI CACGTG 1 cut(s) 257
PspFI CCCAGC 3 cut(s) 1775, 1987, 2032
PspGI CCWGG 4 cut(s) 276, 318, 498, 2482
PspN4I GGNNCC 5 cut(s) 176, 275, 1143, 2434, 2533
PspPI GGNCC 2 cut(s) 160, 727
PspPPI RGGWCCY 1 cut(s) 160
PsrI GAACNNNNNNTAC 2 cut(s) 2186, 2218
PstI CTGCAG 1 cut(s) 2322
PstNI CAGNNNCTG 1 cut(s) 1151
PsuI RGATCY 2 cut(s) 2218, 2284
RsaI GTAC 3 cut(s) 206, 2250, 2533
RsaNI GTAC 3 cut(s) 205, 2249, 2532
RseI CAYNNNNRTG 6 cut(s) 428, 1175, 1308, 1335, 1494, 2026
Sau3AI GATC 4 cut(s) 432, 1666, 2218, 2284
Sau96I GGNCC 2 cut(s) 160, 727
SbfI CCTGCAGG 1 cut(s) 2322
ScrFI CCNGG 4 cut(s) 278, 320, 500, 2484
SdaI CCTGCAGG 1 cut(s) 2322
SduI GDGCHC 4 cut(s) 417, 1682, 1743, 2139
SfaNI GCATC 9 cut(s) 273, 1008, 1138, 1300, 1788, 1825, 1848, 2421, 2533
SfcI CTRYAG 4 cut(s) 844, 1054, 2318, 2424
SfuI TTCGAA 1 cut(s) 1746
SinI GGWCC 2 cut(s) 160, 727
SmiMI CAYNNNNRTG 6 cut(s) 428, 1175, 1308, 1335, 1494, 2026
SmlI CTYRAG 4 cut(s) 884, 1118, 1696, 2393
SmoI CTYRAG 4 cut(s) 884, 1118, 1696, 2393
SnaBI TACGTA 1 cut(s) 1158
Sse8387I CCTGCAGG 1 cut(s) 2322
Sse9I AATT 7 cut(s) 110, 581, 872, 928, 1069, 2344, 2370
SspI AATATT 3 cut(s) 1165, 1243, 1882
SspMI CTAG 2 cut(s) 657, 1356
StyD4I CCNGG 4 cut(s) 276, 318, 498, 2482
StyI CCWWGG 1 cut(s) 1362
TaaI ACNGT 7 cut(s) 190, 640, 1407, 2239, 2366, 2385, 2584
TaiI ACGT 6 cut(s) 219, 259, 947, 1160, 1265, 1640
TaqI TCGA 9 cut(s) 192, 219, 483, 986, 1538, 1686, 1746, 2368, 2415
TasI AATT 7 cut(s) 110, 581, 872, 928, 1069, 2344, 2370
TatI WGTACW 1 cut(s) 204
TauI GCSGC 5 cut(s) 130, 341, 370, 450, 1049
TfiI GAWTC 2 cut(s) 917, 1928
TscAI CASTG 3 cut(s) 535, 753, 1410
TseFI GTSAC 2 cut(s) 184, 1210
Tsp45I GTSAC 2 cut(s) 184, 1210
TspGWI ACGGA 2 cut(s) 130, 172
TspRI CASTG 3 cut(s) 535, 753, 1410
Vha464I CTTAAG 2 cut(s) 1696, 2393
VneI GTGCAC 2 cut(s) 1678, 2135
VpaK11BI GGWCC 2 cut(s) 160, 727
VspI ATTAAT 1 cut(s) 540
XagI CCTNNNNNAGG 2 cut(s) 887, 2481
XapI RAATTY 4 cut(s) 110, 928, 1069, 2370
XceI RCATGY 1 cut(s) 2332
XspI CTAG 2 cut(s) 657, 1356
ZraI GACGTC 1 cut(s) 217
Zsp2I ATGCAT 1 cut(s) 1495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.