pycom06g16220

Pentatricopeptide repeat-containing protein At1g74850

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0009497
Physical Location & Seq
Forward (+)
20585461 .. 20587970
2510 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g16220.1

Sequence Viewer

Length: 1707 bp
ATGAAGAAAGATAAGGTTTCGCCGAGTATTTTGACTTATAATACTGTGCTCAATGCTTGTGCAAGAGGTGGGTTGGATTGGGAAGGGTTGTTAGGATTGTTTGCCGAAATGCGGCACGAAGGAATACAGCCGGACCTTGTTACTTACAATACTTTGCTTAGTGCTTGTGCTGGTAGAGGGTTAGGTGATGAGGCGGAGATGGTGTTCAGGACTATGAATGAGGGTGGGATTGTTCCGGATATAACCACGTATCGTTACCTTGTTGAAACTTTTGGTAAATTGGACAAGCTCGAGAGAGTCTCGGAGCTTCTTAAAGAGATGGAAGCTGGAGGGAATTTTCCTGATATTACGTCGTATAATGTGTTACTAGAGGCATATGCACAATTGGGGTCGATTAGAGAGTCAATGGGTGAGTTTAGGCAGATGCAGGCAGCGGGGTGTATGCCAAATGCCGCCACTTATAGTATTTTGTTGAATCTGTACGGGATTCATGGAAGGTATGATGATGTTCGGGAGCTTTTTCTTGAGATGAAAGTGAGCAATATAGTGCCAGATGCAGCTACGTATAACATTCTCATACAAGTGTTTGGGGCGGGTGGGTATTTTAAGGAGGTAGTGACTTTGTTTCATGATATGGTGGAGGAGAAAATCGAGCCCAATATGGAGACGTACGAAGGGTTGATATATGCTTGTGGAAAGGGAGGACTCCATGAGGATTCCAAGAAAATTTTACTTCACATGCACGAAAATGGAATAGTGCCTAGTACCAAGGTCTATACAGGGGTTATTGAAGCATATGGGCAAGCGGCATTGTATGACGAAGCTCTTGTAGCCTTCAACATAATGAATGAAGTGGGAAGCAAACCATCAGTTGAAAGCTACAACTTGCTGATACATGCATTTGCAAGAGGCGGATTATACAGGGAGACTGAAGCAGTCTTGTTGATAACGGGCGAGGCTGGTGTTGCCAGGAATGTCCATACAGTCAATGGCATGATAGAAGCTTTTAGGCAAGGAGGTCAATTTGAAGAAGCTATCAAGGCATATGTTGAGATGGAAAAGACAAGATGCGAACCCAATGAGTGGACCCTTGAGGCAGTTTTAAGCGTTTACTGCGTTGCAGGTCTTGTTAATGAGTGTGAGAAGCATTTCCAAGAGATTAAAGCCTCAGGAATATTACCCAGTGTCATGTGCTACTGCATGATGCTTGCTGTTTATGCGAGGAGTGACAGGTGGGATGATGCCTATGAGTTGCTGAATGAGTTGCTGAATGGTGCGTATACAGCAATGTCAGTTTGGCTAAACAATATGTATGAGATGTTCCTCAATGGCGAGGATCTACCCCATCTTGCAACTGTCGTCGTAGTACGAGGGAAGGTGGAGAAAAGTTCCATATCACAAGAACTACCGATTGCAAAGGCTGCCTATACATTTCTGCAGGATAATATGCCAGCATCCTTTTCTTTCCCAAAATGGAACAATGGTCGAATACTCTGCCAGCGCCCTCAGCTCAAGCGGATTCTATCAAGCATTGAACCATCCACGGAGGGGTCCGAAAGGAATAATATAACCACGTTAAGTAACTCTCCGTTTCCTCCTCCAGGAACAAAGACATCCTCCAGTGCTGTCAATAGTGGACGGTATAATGATGCCAGTTCGGATGAAAGAACTAGGAGAAGAACAGAGCTTTTGACAAGCCCGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

569

Amino Acids

63.15

Weight (kDa)

5.03

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 1 - 131 1.4e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 8 - 57 1.9e-11 PPR repeat family
TPR_24 PF23276 15 - 141 1.5e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 33 - 93 1.1e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 41 - 72 4e-06 PPR repeat
PPR_2 PF13041 44 - 91 5.8e-11 PPR repeat family
PPR_3 PF13812 103 - 162 2.5e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 114 - 159 3.9e-10 PPR repeat family
PPR_2 PF13041 156 - 196 2.1e-08 PPR repeat family
PPR_3 PF13812 173 - 233 5.4e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 184 - 233 2.3e-10 PPR repeat family
PPR_3 PF13812 208 - 268 1.2e-06 Pentatricopeptide repeat domain
TPR_24 PF23276 242 - 351 1.5e-06 Fungal tetratrico peptide repeats
PPR_2 PF13041 254 - 303 2.8e-06 PPR repeat family
PPR_2 PF13041 324 - 372 4.6e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 39
Acc36I ACCTGC 1 cut(s) 1112
AccB7I CCANNNNNTGG 1 cut(s) 1083
AccI GTMKAC 1 cut(s) 1280
AccIII TCCGGA 1 cut(s) 235
AciI CCGC 8 cut(s) 112, 194, 434, 453, 593, 806, 912, 1516
AclWI GGATC 1 cut(s) 1344
AcsI RAATTY 2 cut(s) 334, 726
AcuI CTGAAG 1 cut(s) 951
AfaI GTAC 4 cut(s) 482, 671, 766, 1368
AfiI CCNNNNNNNGG 4 cut(s) 111, 1083, 1548, 1601
AgsI TTSAA 7 cut(s) 266, 475, 791, 838, 875, 1028, 1535
AjnI CCWGG 2 cut(s) 968, 1600
AloI GAACNNNNNNTCC 2 cut(s) 188, 220
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 3 cut(s) 304, 659, 920
AlwI GGATC 1 cut(s) 1344
Ama87I CYCGRG 1 cut(s) 290
Aor13HI TCCGGA 1 cut(s) 235
ApeKI GCWGC 3 cut(s) 431, 557, 1421
ApoI RAATTY 2 cut(s) 334, 726
Asp700I GAANNNNTTC 1 cut(s) 1148
AspLEI GCGC 1 cut(s) 1503
AspS9I GGNCC 3 cut(s) 133, 1086, 1551
AsuC2I CCSGG 1 cut(s) 1700
AsuHPI GGTGA 2 cut(s) 197, 422
AvaI CYCGRG 1 cut(s) 290
AvaII GGWCC 3 cut(s) 133, 1086, 1551
AxyI CCTNAGG 1 cut(s) 1168
BanII GRGCYC 1 cut(s) 657
Bbv12I GWGCWC 1 cut(s) 51
BbvCI CCTCAGC 1 cut(s) 1506
BbvI GCAGC 3 cut(s) 443, 569, 1408
BccI CCATC 6 cut(s) 193, 313, 874, 1048, 1353, 1546
BcgI CGANNNNNNTGC 2 cut(s) 1476, 1510
BciT130I CCWGG 2 cut(s) 970, 1602
BcnI CCSGG 1 cut(s) 1700
BcoDI GTCTC 3 cut(s) 304, 659, 920
BfaI CTAG 3 cut(s) 368, 762, 1671
BfmI CTRYAG 1 cut(s) 1436
BfoI RGCGCY 1 cut(s) 1504
BfuAI ACCTGC 1 cut(s) 1112
BisI GCNGC 6 cut(s) 113, 432, 453, 558, 807, 1422
BlsI GCNGC 6 cut(s) 114, 433, 454, 559, 808, 1423
Bme1390I CCNGG 3 cut(s) 970, 1602, 1700
Bme18I GGWCC 3 cut(s) 133, 1086, 1551
BmeT110I CYCGRG 1 cut(s) 290
BmgT120I GGNCC 3 cut(s) 133, 1086, 1551
BmiI GGNNCC 2 cut(s) 1088, 1552
BmrFI CCNGG 3 cut(s) 970, 1602, 1700
BmrI ACTGGG 1 cut(s) 1176
BmsI GCATC 7 cut(s) 414, 544, 1058, 1194, 1231, 1463, 1639
BmuI ACTGGG 1 cut(s) 1176
BplI GAGNNNNNCTC 2 cut(s) 284, 316
BpmI CTGGAG 3 cut(s) 348, 1584, 1603
Bpu10I CCTNAGC 1 cut(s) 1506
BpuEI CTTGAG 3 cut(s) 545, 1112, 1496
BpuMI CCSGG 1 cut(s) 1700
BsaAI YACGTR 2 cut(s) 249, 564
BsaJI CCNNGG 2 cut(s) 768, 1542
BsaWI WCCGGW 1 cut(s) 235
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bsc4I CCNNNNNNNGG 4 cut(s) 111, 1083, 1548, 1601
Bse1I ACTGG 3 cut(s) 1182, 1620, 1653
Bse21I CCTNAGG 1 cut(s) 1168
Bse3DI GCAATG 1 cut(s) 1293
BseAI TCCGGA 1 cut(s) 235
BseBI CCWGG 2 cut(s) 970, 1602
BseDI CCNNGG 2 cut(s) 768, 1542
BseGI GGATG 5 cut(s) 1243, 1454, 1538, 1613, 1666
BseLI CCNNNNNNNGG 4 cut(s) 111, 1083, 1548, 1601
BseMI GCAATG 1 cut(s) 1293
BseMII CTCAG 2 cut(s) 1182, 1520
BseNI ACTGG 3 cut(s) 1182, 1620, 1653
BseRI GAGGAG 3 cut(s) 656, 1237, 1587
BseXI GCAGC 3 cut(s) 443, 569, 1408
BsiHKAI GWGCWC 1 cut(s) 51
BsiHKCI CYCGRG 1 cut(s) 290
BsiSI CCGG 3 cut(s) 131, 236, 1700
BsiWI CGTACG 1 cut(s) 669
BslI CCNNNNNNNGG 4 cut(s) 111, 1083, 1548, 1601
BsmAI GTCTC 3 cut(s) 304, 659, 920
BsmBI CGTCTC 1 cut(s) 659
BsoBI CYCGRG 1 cut(s) 290
Bsp1286I GDGCHC 2 cut(s) 51, 657
Bsp13I TCCGGA 1 cut(s) 235
Bsp143I GATC 1 cut(s) 1336
BspACI CCGC 8 cut(s) 112, 194, 434, 453, 593, 806, 912, 1516
BspCNI CTCAG 2 cut(s) 1181, 1519
BspEI TCCGGA 1 cut(s) 235
BspHI TCATGA 1 cut(s) 628
BspLI GGNNCC 2 cut(s) 1088, 1552
BspMAI CTGCAG 1 cut(s) 1440
BspMI ACCTGC 1 cut(s) 1112
BspPI GGATC 1 cut(s) 1344
BsrDI GCAATG 1 cut(s) 1293
BsrI ACTGG 3 cut(s) 1182, 1620, 1653
BssECI CCNNGG 2 cut(s) 768, 1542
BssMI GATC 1 cut(s) 1336
BssNAI GTATAC 1 cut(s) 1281
BssT1I CCWWGG 1 cut(s) 768
Bst1107I GTATAC 1 cut(s) 1281
Bst2UI CCWGG 2 cut(s) 970, 1602
Bst4CI ACNGT 4 cut(s) 46, 985, 1357, 1641
BstAPI GCANNNNNTGC 1 cut(s) 1421
BstBAI YACGTR 2 cut(s) 249, 564
BstC8I GCNNGC 5 cut(s) 429, 804, 1209, 1452, 1499
BstDEI CTNAG 3 cut(s) 158, 1168, 1506
BstDSI CCRYGG 1 cut(s) 1542
BstENI CCTNNNNNAGG 1 cut(s) 1599
BstF5I GGATG 5 cut(s) 1243, 1454, 1538, 1613, 1666
BstH2I RGCGCY 1 cut(s) 1504
BstHHI GCGC 1 cut(s) 1503
BstKTI GATC 1 cut(s) 1339
BstMAI GTCTC 3 cut(s) 304, 659, 920
BstMBI GATC 1 cut(s) 1336
BstMWI GCNNNNNNNGC 8 cut(s) 830, 965, 1040, 1113, 1217, 1283, 1421, 1507
BstNI CCWGG 2 cut(s) 970, 1602
BstNSI RCATGY 2 cut(s) 742, 899
BstSCI CCNGG 3 cut(s) 968, 1600, 1698
BstSFI CTRYAG 1 cut(s) 1436
BstSNI TACGTA 1 cut(s) 564
BstV1I GCAGC 3 cut(s) 443, 569, 1408
BstX2I RGATCY 1 cut(s) 1336
BstYI RGATCY 1 cut(s) 1336
BstZ17I GTATAC 1 cut(s) 1281
Bsu36I CCTNAGG 1 cut(s) 1168
BtgI CCRYGG 1 cut(s) 1542
BtsCI GGATG 5 cut(s) 1243, 1454, 1538, 1613, 1666
BtsIMutI CAGTG 2 cut(s) 1189, 1627
BveI ACCTGC 1 cut(s) 1112
Cac8I GCNNGC 5 cut(s) 429, 804, 1209, 1452, 1499
CciI TCATGA 1 cut(s) 628
CfoI GCGC 1 cut(s) 1503
Cfr13I GGNCC 3 cut(s) 133, 1086, 1551
Csp6I GTAC 4 cut(s) 481, 670, 765, 1367
CviAII CATG 8 cut(s) 491, 629, 710, 739, 896, 994, 1189, 1201
CviQI GTAC 4 cut(s) 481, 670, 765, 1367
DdeI CTNAG 3 cut(s) 158, 1168, 1506
DpnI GATC 1 cut(s) 1338
DpnII GATC 1 cut(s) 1336
EciI GGCGGA 2 cut(s) 209, 927
Eco105I TACGTA 1 cut(s) 564
Eco130I CCWWGG 1 cut(s) 768
Eco24I GRGCYC 1 cut(s) 657
Eco47I GGWCC 3 cut(s) 133, 1086, 1551
Eco57I CTGAAG 1 cut(s) 951
Eco81I CCTNAGG 1 cut(s) 1168
Eco88I CYCGRG 1 cut(s) 290
EcoNI CCTNNNNNAGG 1 cut(s) 1599
EcoRII CCWGG 2 cut(s) 968, 1600
EcoT14I CCWWGG 1 cut(s) 768
EcoT22I ATGCAT 1 cut(s) 901
EcoT38I GRGCYC 1 cut(s) 657
ErhI CCWWGG 1 cut(s) 768
Esp3I CGTCTC 1 cut(s) 659
FaeI CATG 8 cut(s) 494, 632, 713, 742, 899, 997, 1192, 1204
FatI CATG 8 cut(s) 490, 628, 709, 738, 895, 993, 1188, 1200
FauI CCCGC 2 cut(s) 427, 586
FauNDI CATATG 3 cut(s) 376, 796, 1045
FblI GTMKAC 1 cut(s) 1280
Fnu4HI GCNGC 6 cut(s) 113, 432, 453, 558, 807, 1422
FokI GGATG 5 cut(s) 1250, 1441, 1525, 1600, 1673
FriOI GRGCYC 1 cut(s) 657
Fsp4HI GCNGC 6 cut(s) 113, 432, 453, 558, 807, 1422
FspBI CTAG 3 cut(s) 368, 762, 1671
GlaI GCGC 1 cut(s) 1502
GluI GCNGC 6 cut(s) 113, 432, 453, 558, 807, 1422
GsuI CTGGAG 3 cut(s) 348, 1584, 1603
HaeII RGCGCY 1 cut(s) 1504
HapII CCGG 3 cut(s) 131, 236, 1700
HhaI GCGC 1 cut(s) 1503
Hin1II CATG 8 cut(s) 494, 632, 713, 742, 899, 997, 1192, 1204
Hin6I GCGC 1 cut(s) 1501
HinP1I GCGC 1 cut(s) 1501
HindIII AAGCTT 1 cut(s) 1002
HinfI GANTC 7 cut(s) 297, 401, 475, 487, 705, 716, 1519
HpaII CCGG 3 cut(s) 131, 236, 1700
HphI GGTGA 2 cut(s) 197, 422
Hpy166II GTNNAC 4 cut(s) 1086, 1111, 1281, 1637
Hpy188I TCNGA 3 cut(s) 304, 1555, 1660
Hpy188III TCNNGA 8 cut(s) 208, 236, 292, 341, 512, 524, 629, 1170
Hpy8I GTNNAC 4 cut(s) 1086, 1111, 1281, 1637
Hpy99I CGWCG 2 cut(s) 355, 1364
HpyAV CCTTC 6 cut(s) 77, 113, 489, 668, 844, 1369
HpyCH4III ACNGT 4 cut(s) 46, 985, 1357, 1641
HpyCH4IV ACGT 5 cut(s) 248, 350, 563, 668, 1574
HpyF10VI GCNNNNNNNGC 8 cut(s) 830, 965, 1040, 1113, 1217, 1283, 1421, 1507
HpyF3I CTNAG 3 cut(s) 158, 1168, 1506
HpySE526I ACGT 5 cut(s) 248, 350, 563, 668, 1574
Hsp92II CATG 8 cut(s) 494, 632, 713, 742, 899, 997, 1192, 1204
HspAI GCGC 1 cut(s) 1501
Kpn2I TCCGGA 1 cut(s) 235
Kzo9I GATC 1 cut(s) 1336
LmnI GCTCC 2 cut(s) 304, 514
Lsp1109I GCAGC 3 cut(s) 443, 569, 1408
LweI GCATC 7 cut(s) 414, 544, 1058, 1194, 1231, 1463, 1639
MaeI CTAG 3 cut(s) 368, 762, 1671
MaeII ACGT 5 cut(s) 248, 350, 563, 668, 1574
MaeIII GTNAC 6 cut(s) 139, 254, 363, 616, 1226, 1580
MalI GATC 1 cut(s) 1338
MboI GATC 1 cut(s) 1336
MboII GAAGA 3 cut(s) 16, 1040, 1689
MfeI CAATTG 1 cut(s) 383
MflI RGATCY 1 cut(s) 1336
MhlI GDGCHC 2 cut(s) 51, 657
MluCI AATT 5 cut(s) 278, 334, 383, 726, 1022
MlyI GAGTC 3 cut(s) 306, 410, 699
MmeI TCCRAC 1 cut(s) 54
Mph1103I ATGCAT 1 cut(s) 901
MroI TCCGGA 1 cut(s) 235
MroXI GAANNNNTTC 1 cut(s) 1148
MseI TTAA 7 cut(s) 312, 606, 1103, 1131, 1161, 1577, 1705
MslI CAYNNNNRTG 2 cut(s) 581, 747
MspA1I CMGCKG 1 cut(s) 434
MspI CCGG 3 cut(s) 131, 236, 1700
MspR9I CCNGG 3 cut(s) 970, 1602, 1700
MunI CAATTG 1 cut(s) 383
MvaI CCWGG 2 cut(s) 970, 1602
MwoI GCNNNNNNNGC 8 cut(s) 830, 965, 1040, 1113, 1217, 1283, 1421, 1507
NciI CCSGG 1 cut(s) 1700
NdeI CATATG 3 cut(s) 376, 796, 1045
NdeII GATC 1 cut(s) 1336
NlaIII CATG 8 cut(s) 494, 632, 713, 742, 899, 997, 1192, 1204
NlaIV GGNNCC 2 cut(s) 1088, 1552
NmeAIII GCCGAG 1 cut(s) 48
NmuCI GTSAC 2 cut(s) 616, 1226
NsiI ATGCAT 1 cut(s) 901
NspI RCATGY 2 cut(s) 742, 899
PaeR7I CTCGAG 1 cut(s) 290
PagI TCATGA 1 cut(s) 628
PdmI GAANNNNTTC 1 cut(s) 1148
PfeI GAWTC 4 cut(s) 475, 487, 716, 1519
Pfl23II CGTACG 1 cut(s) 669
PflMI CCANNNNNTGG 1 cut(s) 1083
PfoI TCCNGGA 1 cut(s) 1600
PkrI GCNGC 6 cut(s) 114, 433, 454, 559, 808, 1423
PleI GAGTC 3 cut(s) 305, 409, 699
PpsI GAGTC 3 cut(s) 305, 409, 699
Ppu21I YACGTR 2 cut(s) 249, 564
PsiI TTATAA 1 cut(s) 39
Psp6I CCWGG 2 cut(s) 968, 1600
PspGI CCWGG 2 cut(s) 968, 1600
PspLI CGTACG 1 cut(s) 669
PspN4I GGNNCC 2 cut(s) 1088, 1552
PspPI GGNCC 3 cut(s) 133, 1086, 1551
PsrI GAACNNNNNNTAC 2 cut(s) 1304, 1336
PstI CTGCAG 1 cut(s) 1440
PsuI RGATCY 1 cut(s) 1336
RsaI GTAC 4 cut(s) 482, 671, 766, 1368
RsaNI GTAC 4 cut(s) 481, 670, 765, 1367
RseI CAYNNNNRTG 2 cut(s) 581, 747
SaqAI TTAA 7 cut(s) 312, 606, 1103, 1131, 1161, 1577, 1705
SatI GCNGC 6 cut(s) 113, 432, 453, 558, 807, 1422
Sau3AI GATC 1 cut(s) 1336
Sau96I GGNCC 3 cut(s) 133, 1086, 1551
SchI GAGTC 3 cut(s) 306, 410, 699
ScrFI CCNGG 3 cut(s) 970, 1602, 1700
SduI GDGCHC 2 cut(s) 51, 657
SfaNI GCATC 7 cut(s) 414, 544, 1058, 1194, 1231, 1463, 1639
SfcI CTRYAG 1 cut(s) 1436
Sfr274I CTCGAG 1 cut(s) 290
SinI GGWCC 3 cut(s) 133, 1086, 1551
SlaI CTCGAG 1 cut(s) 290
SmiMI CAYNNNNRTG 2 cut(s) 581, 747
SmlI CTYRAG 4 cut(s) 290, 524, 1091, 1511
SmoI CTYRAG 4 cut(s) 290, 524, 1091, 1511
SnaBI TACGTA 1 cut(s) 564
Sse9I AATT 5 cut(s) 278, 334, 383, 726, 1022
SsiI CCGC 8 cut(s) 112, 194, 434, 453, 593, 806, 912, 1516
SspI AATATT 1 cut(s) 1176
SspMI CTAG 3 cut(s) 368, 762, 1671
StyD4I CCNGG 3 cut(s) 968, 1600, 1698
StyI CCWWGG 1 cut(s) 768
TaaI ACNGT 4 cut(s) 46, 985, 1357, 1641
TaiI ACGT 5 cut(s) 251, 353, 566, 671, 1577
TaqI TCGA 4 cut(s) 291, 392, 651, 1486
TasI AATT 5 cut(s) 278, 334, 383, 726, 1022
TauI GCSGC 3 cut(s) 115, 455, 809
TfiI GAWTC 4 cut(s) 475, 487, 716, 1519
Tru1I TTAA 7 cut(s) 312, 606, 1103, 1131, 1161, 1577, 1705
Tru9I TTAA 7 cut(s) 312, 606, 1103, 1131, 1161, 1577, 1705
TscAI CASTG 2 cut(s) 1189, 1627
TseFI GTSAC 2 cut(s) 616, 1226
TseI GCWGC 3 cut(s) 431, 557, 1421
Tsp45I GTSAC 2 cut(s) 616, 1226
TspDTI ATGAA 8 cut(s) 17, 230, 479, 545, 617, 860, 864, 1677
TspGWI ACGGA 2 cut(s) 1559, 1578
TspRI CASTG 2 cut(s) 1189, 1627
Van91I CCANNNNNTGG 1 cut(s) 1083
VpaK11BI GGWCC 3 cut(s) 133, 1086, 1551
XagI CCTNNNNNAGG 1 cut(s) 1599
XapI RAATTY 2 cut(s) 334, 726
XceI RCATGY 2 cut(s) 742, 899
XcmI CCANNNNNNNNNTGG 1 cut(s) 986
XhoI CTCGAG 1 cut(s) 290
XmiI GTMKAC 1 cut(s) 1280
XmnI GAANNNNTTC 1 cut(s) 1148
XspI CTAG 3 cut(s) 368, 762, 1671
Zsp2I ATGCAT 1 cut(s) 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.