Rw7G002390

Pentatricopeptide repeat-containing protein At1g74850

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0009497
Physical Location & Seq
Forward (+)
1949146 .. 1952989
3844 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G002390.1

Sequence Viewer

Length: 2556 bp
ATGACCCTCTCCATTTCCATCCCCTCCCCTCTCTCCGCCCCAATCCTCAAGCTAAACCCTAAACCCCACCACCTCTCCTCCTTCCGATTCCTCTCCGGCCACCGCAAGTTCCTCCACGGCCAGCGGTTATCGGTCTCCGTCAGGGCGAAGCCGAAGGACCTCATCCTCGGGAACCCCTCCGTGACCGTCGAGAAGGGCAAGTACAGCTACGACGTCGAAACCCTAATCAACAAGCTCAGCAGCCTCCCGCCACGTGGCAGCATCGCGCGGTGCCTGGACATCTTCAAGAACAAGCTCTCCCTGAACGACTTCGCCCTGGTGTTCAAGGAGTTCGCGGCGCGTGGCGACTGGCAGAGGTCGCTGCGGCTGTTCAAGTATATGCAGCGGCAGATATGGTGCAAGCCGAGCGAGCACATCTACACCATCATGATCAGTCTGCTCGGCCGCGAAGGTTTACTTGACAAATGCGCTGAGATTTTCGACGAAATGCCTAGCCAAGGTGTGATTAGGACTGTGTTTAGCTACACTGCTTTGATTAATGCCTATGGCCGGAATGGTCAGTATGAAATGTCTCTTCAATTACTTGATAGAATGAAGAAAGATAGGGTTTCGCCGAATATATTGACTTATAATACTGTGCTTAATGCTTGTGCTAGAGGGGGGTTGGATTGGGAGGGGTTGTTAGGATTGTTTGCGGAAATGAGGCACGAGGGGGTTCAGCCGGACCTTGTTACTTATAATACATTGCTTAGTGCTTGCGCCGGGAGAGGTTTAGGTGATGAGGCGGAGATGGTGTTTAGGACTATGAATGAGGGTGGGATAGTTCCGGATATAACCACGTATAGTTATCTTGTTGAGACTTTTGGTAAATTGGATAAGCTTGAGAAGGTTTCCGAGTTGCTTAAAGGGATGGAGTCTGGGGGGAATTTGCCTGATATTACGTCGTATAATGTGTTGTTGGAGGCGTATGCGCAGTTGGGGCAGATGCAAGCGGCGGGGTGTATGCCGAATGCGGCTACTTATAGTATTTTGTTGAATTTGTATGGGAGGCATGGGAGGTATGATGATGTTCGGGAGCTTTTTCTTGAGATGAAAGTGAGCAATACGGAGCCAGATGTTGCTACGTATAATATTCTCATACAGGTGTTTGGTGAGGGTGGGTATTTTAGGGAGGTGGTGACTTTGTTTCATGATATGGTAGAGGAGAATATTGAGCCGAATATGGAGACGTATGAAGGATTGATATATGCTTGTGGAAAAGGAGGGCTCCATGAGGATGCCAAGAACATATTGCTTCACATGAATGATAAGGGAATAGTGCCTAGTTCCAAGGCTTATACAGGAGTTATTGAAGCATATGGGCAAGCAGCACTGTATGATGAAGCTCTTGTTGCCTTCAACACAATGCATGAAGTGGGCAGCAGACCGTCAGTTGAAAGCTACAACTCGCTTATTCATGCATACGCAAGAGGCGGACTATACAAGGAGACTGAACAAATCTTGTCGATAATGGGTGAGGTTGGCATTGCAAGGAATGCCAGTTCATTCAATGGAATGATTGAAGCTTTTAGGCAGGGGGGTCAGTTTGAAGAAGCTATAAAGACATACGTTGAGATGGAAAAGAGAAGATGTGATCCCGATGAGTGCACTCTTGAAGCAGTCTTAAGCGTTTATAGTGTTGCAGGTCTTGTTAATGAGTGTGAGGAGCACTTCGAAGAGATTAAAGCTTCAGGGATATTGCCCAGCGTCATGTGCTACTGCATGATGCTTGCTGTTTATGCAAAGACTGACAGGTGGGATGATGCCAATAAGTTGCTGAATGAGATGCTCACAAATAGGGTATCAAATATTCATCAAGTGATGGGACAGATGATAAAGGGAGATTATGATGACGAATCTAACTGGCAGATGGTAGAGTATGTTTTTGACAAACTAAAATCTGAAGGATGTGGGCTCGGAATGAGGTTCTACAACACTTTGATAGAAGCACTTTGGTGGCTGGGTCAGAAACAAAGAGCTGTGCGCGTTCTTAGTGAAGCAACTCAACGGGGGTTATTTCCTGAACTTCTCCGTAAAAATAAACTTGTTTGGTCTATAGATGTGCACAGGATGTGGGAAGGTGGTGCGTATGCAGCAATGTCACTTTGGCTAAACAATATGTATGAGATGTTCCTGAATGGCGAGGATCTTCCCTATGTTGCAACTGTTGTTGTAGTACGGGGGAAGATGGAAAAAAATTCAGCAACGCAAGATCTGCCGGTTGCAAAGGCCGCCTATTCATTCCTGCAGGATAACATGTCAGGAGCATTCAGTTTCCCAAAATGGAACAATGGTCGGATTCTCTGCCAAAGGTCTCAGCTCAAGAAGATTCTGTCGAGCATTGAACCATCTACAGACGGCTCCAGCAGTAAAAGTATATGTACTTTAAGTAACTCCCCTTTTCCTCCTCCAGGCACAAAAACATCACACACCGATGTTGATAGTGTTCTGTATAATGGTACCACTTCTGATGCAACAATTAGGACAAGAACAGAGCTTTTGACAAGCACAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

851

Amino Acids

95.41

Weight (kDa)

5.73

Isoelectric Point (pI)

43.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 117 - 219 8.9e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 135 - 179 1.3e-06 PPR repeat family
PPR PF01535 139 - 168 4.8e-06 PPR repeat
PPR_3 PF13812 159 - 217 6.6e-15 Pentatricopeptide repeat domain
PPR_2 PF13041 171 - 219 2.8e-14 PPR repeat family
PPR PF01535 173 - 202 1.1e-07 PPR repeat
PPR_long PF17177 189 - 329 3.4e-11 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 213 - 332 4.8e-07 Fungal tetratrico peptide repeats
PPR_2 PF13041 228 - 254 4.1e-06 PPR repeat family
PPR_3 PF13812 230 - 290 6.9e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 238 - 269 7.1e-06 PPR repeat
PPR_2 PF13041 241 - 288 2.7e-11 PPR repeat family
PPR_3 PF13812 328 - 385 7.6e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 336 - 383 1e-12 PPR repeat family
PPR PF01535 340 - 367 6e-06 PPR repeat
PPR_2 PF13041 371 - 420 3e-11 PPR repeat family
PPR_3 PF13812 395 - 455 3.2e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 441 - 490 3.7e-06 PPR repeat family
PPR_3 PF13812 468 - 523 3.9e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 512 - 552 3e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 630, 738
AatII GACGTC 1 cut(s) 216
Acc16I TGCGCA 1 cut(s) 972
Acc36I ACCTGC 1 cut(s) 1673
Acc65I GGTACC 1 cut(s) 2498
AccB1I GGYRCC 2 cut(s) 270, 2498
AccII CGCG 6 cut(s) 266, 268, 335, 340, 447, 2025
AccIII TCCGGA 1 cut(s) 826
AclWI GGATC 2 cut(s) 1628, 2193
AcoI YGGCCR 4 cut(s) 97, 118, 442, 547
AcsI RAATTY 3 cut(s) 925, 1036, 2236
AcuI CTGAAG 2 cut(s) 1713, 1962
AcvI CACGTG 1 cut(s) 254
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 4 cut(s) 203, 2217, 2422, 2500
AfiI CCNNNNNNNGG 4 cut(s) 254, 497, 549, 2450
AflII CTTAAG 1 cut(s) 1663
AflIII ACRYGT 1 cut(s) 2295
AjnI CCWGG 3 cut(s) 273, 315, 2449
AleI CACNNNNGTG 1 cut(s) 1993
AloI GAACNNNNNNTCC 4 cut(s) 281, 313, 1525, 1557
Alw21I GWGCWC 4 cut(s) 414, 1649, 1710, 2106
Alw26I GTCTC 6 cut(s) 139, 576, 851, 1220, 1481, 2358
Alw44I GTGCAC 2 cut(s) 1645, 2102
AlwI GGATC 2 cut(s) 1628, 2193
Ama87I CYCGRG 1 cut(s) 167
Aor13HI TCCGGA 1 cut(s) 826
AoxI GGCC 5 cut(s) 97, 118, 442, 547, 2268
ApaLI GTGCAC 2 cut(s) 1645, 2102
ApeKI GCWGC 7 cut(s) 240, 258, 361, 382, 1367, 1419, 2132
ApoI RAATTY 3 cut(s) 925, 1036, 2236
AseI ATTAAT 1 cut(s) 537
Asp700I GAANNNNTTC 1 cut(s) 308
Asp718I GGTACC 1 cut(s) 2498
AspLEI GCGC 6 cut(s) 268, 340, 470, 761, 973, 2025
AspS9I GGNCC 2 cut(s) 157, 724
AsuC2I CCSGG 1 cut(s) 763
AsuHPI GGTGA 4 cut(s) 788, 1163, 1189, 1526
AsuII TTCGAA 1 cut(s) 1713
AvaI CYCGRG 1 cut(s) 167
AvaII GGWCC 2 cut(s) 157, 724
BaeGI GKGCMC 2 cut(s) 1649, 2106
BanI GGYRCC 2 cut(s) 270, 2498
BanII GRGCYC 2 cut(s) 1269, 1956
BarI GAAGNNNNNNTAC 2 cut(s) 185, 217
BauI CACGAG 1 cut(s) 707
BbrPI CACGTG 1 cut(s) 254
Bbv12I GWGCWC 4 cut(s) 414, 1649, 1710, 2106
BbvI GCAGC 7 cut(s) 252, 270, 348, 394, 1379, 1431, 2144
BccI CCATC 9 cut(s) 26, 431, 784, 904, 1609, 1855, 1903, 2221, 2395
BceAI ACGGC 2 cut(s) 133, 2413
BciT130I CCWGG 3 cut(s) 275, 317, 2451
BclI TGATCA 1 cut(s) 429
BcnI CCSGG 1 cut(s) 763
BcoDI GTCTC 6 cut(s) 139, 576, 851, 1220, 1481, 2358
BfaI CTAG 3 cut(s) 492, 654, 1323
BfmI CTRYAG 3 cut(s) 2094, 2285, 2391
BfrI CTTAAG 1 cut(s) 1663
BfuAI ACCTGC 1 cut(s) 1673
BglII AGATCT 1 cut(s) 2251
BlpI GCTNAGC 1 cut(s) 236
Bme1390I CCNGG 4 cut(s) 275, 317, 763, 2451
Bme18I GGWCC 2 cut(s) 157, 724
BmeT110I CYCGRG 1 cut(s) 167
BmgT120I GGNCC 2 cut(s) 157, 724
BmiI GGNNCC 6 cut(s) 173, 272, 1110, 1268, 2401, 2500
BmrFI CCNGG 4 cut(s) 275, 317, 763, 2451
BmsI GCATC 7 cut(s) 270, 975, 1267, 1755, 1792, 1815, 2500
BpmI CTGGAG 2 cut(s) 2386, 2433
Bpu1102I GCTNAGC 1 cut(s) 236
Bpu14I TTCGAA 1 cut(s) 1713
BpuEI CTTGAG 4 cut(s) 32, 902, 1106, 2345
BpuMI CCSGG 1 cut(s) 763
BsaAI YACGTR 3 cut(s) 254, 840, 1125
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 2 cut(s) 139, 2358
BsaJI CCNNGG 5 cut(s) 115, 166, 315, 496, 1329
BsaWI WCCGGW 1 cut(s) 826
Bsc4I CCNNNNNNNGG 4 cut(s) 254, 497, 549, 2450
Bse118I RCCGGY 1 cut(s) 2257
Bse1I ACTGG 3 cut(s) 353, 1539, 1907
Bse3DI GCAATG 3 cut(s) 743, 1524, 2142
BseAI TCCGGA 1 cut(s) 826
BseBI CCWGG 3 cut(s) 275, 317, 2451
BseDI CCNNGG 5 cut(s) 115, 166, 315, 496, 1329
BseGI GGATG 7 cut(s) 18, 162, 915, 1282, 1804, 1952, 2115
BseLI CCNNNNNNNGG 4 cut(s) 254, 497, 549, 2450
BseMI GCAATG 3 cut(s) 743, 1524, 2142
BseMII CTCAG 3 cut(s) 250, 462, 2369
BseNI ACTGG 3 cut(s) 353, 1539, 1907
BseRI GAGGAG 4 cut(s) 67, 1217, 1718, 2436
BseSI GKGCMC 2 cut(s) 1649, 2106
BseX3I CGGCCG 1 cut(s) 442
BseXI GCAGC 7 cut(s) 252, 270, 348, 394, 1379, 1431, 2144
BseYI CCCAGC 2 cut(s) 1742, 1999
Bsh1236I CGCG 6 cut(s) 266, 268, 335, 340, 447, 2025
Bsh1285I CGRYCG 1 cut(s) 445
BshFI GGCC 5 cut(s) 99, 120, 444, 549, 2270
BshNI GGYRCC 2 cut(s) 270, 2498
BsiEI CGRYCG 1 cut(s) 445
BsiHKAI GWGCWC 4 cut(s) 414, 1649, 1710, 2106
BsiHKCI CYCGRG 1 cut(s) 167
BsiSI CCGG 6 cut(s) 96, 550, 722, 762, 827, 2258
BslFI GGGAC 1 cut(s) 1878
BslI CCNNNNNNNGG 4 cut(s) 254, 497, 549, 2450
BsmAI GTCTC 6 cut(s) 139, 576, 851, 1220, 1481, 2358
BsmBI CGTCTC 1 cut(s) 1220
BsmFI GGGAC 1 cut(s) 1878
BsmI GAATGC 3 cut(s) 1015, 1540, 2306
BsnI GGCC 5 cut(s) 99, 120, 444, 549, 2270
Bso31I GGTCTC 2 cut(s) 139, 2358
BsoBI CYCGRG 1 cut(s) 167
Bsp119I TTCGAA 1 cut(s) 1713
Bsp1286I GDGCHC 6 cut(s) 414, 1269, 1649, 1710, 1956, 2106
Bsp13I TCCGGA 1 cut(s) 826
Bsp143I GATC 4 cut(s) 429, 1633, 2185, 2251
Bsp1720I GCTNAGC 1 cut(s) 236
BspANI GGCC 5 cut(s) 99, 120, 444, 549, 2270
BspCNI CTCAG 3 cut(s) 249, 463, 2368
BspEI TCCGGA 1 cut(s) 826
BspFNI CGCG 6 cut(s) 266, 268, 335, 340, 447, 2025
BspHI TCATGA 2 cut(s) 426, 1189
BspLI GGNNCC 6 cut(s) 173, 272, 1110, 1268, 2401, 2500
BspMAI CTGCAG 1 cut(s) 2289
BspMI ACCTGC 1 cut(s) 1673
BspPI GGATC 2 cut(s) 1628, 2193
BspT104I TTCGAA 1 cut(s) 1713
BspT107I GGYRCC 2 cut(s) 270, 2498
BspTI CTTAAG 1 cut(s) 1663
BspTNI GGTCTC 2 cut(s) 139, 2358
BsrDI GCAATG 3 cut(s) 743, 1524, 2142
BsrFI RCCGGY 1 cut(s) 2257
BsrI ACTGG 3 cut(s) 353, 1539, 1907
BssAI RCCGGY 1 cut(s) 2257
BssECI CCNNGG 5 cut(s) 115, 166, 315, 496, 1329
BssMI GATC 4 cut(s) 429, 1633, 2185, 2251
BssNI GRCGYC 1 cut(s) 213
BssSI CACGAG 1 cut(s) 707
BssT1I CCWWGG 2 cut(s) 496, 1329
Bst2BI CACGAG 1 cut(s) 707
Bst2UI CCWGG 3 cut(s) 275, 317, 2451
Bst4CI ACNGT 7 cut(s) 187, 514, 637, 1374, 1428, 2206, 2551
Bst6I CTCTTC 2 cut(s) 579, 1710
BstACI GRCGYC 1 cut(s) 213
BstAFI CTTAAG 1 cut(s) 1663
BstAPI GCANNNNNTGC 2 cut(s) 1535, 2254
BstBAI YACGTR 3 cut(s) 254, 840, 1125
BstBI TTCGAA 1 cut(s) 1713
BstC8I GCNNGC 7 cut(s) 122, 401, 410, 757, 990, 1365, 1770
BstDEI CTNAG 5 cut(s) 236, 471, 749, 2030, 2355
BstDSI CCRYGG 1 cut(s) 115
BstENI CCTNNNNNAGG 2 cut(s) 495, 2448
BstF5I GGATG 7 cut(s) 18, 162, 915, 1282, 1804, 1952, 2115
BstFNI CGCG 6 cut(s) 266, 268, 335, 340, 447, 2025
BstHHI GCGC 6 cut(s) 268, 340, 470, 761, 973, 2025
BstKTI GATC 4 cut(s) 432, 1636, 2188, 2254
BstMAI GTCTC 6 cut(s) 139, 576, 851, 1220, 1481, 2358
BstMBI GATC 4 cut(s) 429, 1633, 2185, 2251
BstMCI CGRYCG 1 cut(s) 445
BstNI CCWGG 3 cut(s) 275, 317, 2451
BstNSI RCATGY 1 cut(s) 2299
BstSCI CCNGG 4 cut(s) 273, 315, 761, 2449
BstSFI CTRYAG 3 cut(s) 2094, 2285, 2391
BstSLI GKGCMC 2 cut(s) 1649, 2106
BstSNI TACGTA 1 cut(s) 1125
BstUI CGCG 6 cut(s) 266, 268, 335, 340, 447, 2025
BstV1I GCAGC 7 cut(s) 252, 270, 348, 394, 1379, 1431, 2144
BstX2I RGATCY 2 cut(s) 2185, 2251
BstYI RGATCY 2 cut(s) 2185, 2251
BstZI CGGCCG 1 cut(s) 442
BsuRI GGCC 5 cut(s) 99, 120, 444, 549, 2270
BtgI CCRYGG 1 cut(s) 115
BtgZI GCGATG 1 cut(s) 247
BtsCI GGATG 7 cut(s) 18, 162, 915, 1282, 1804, 1952, 2115
BtsI GCAGTG 1 cut(s) 525
BtsIMutI CAGTG 2 cut(s) 525, 1370
BveI ACCTGC 1 cut(s) 1673
Cac8I GCNNGC 7 cut(s) 122, 401, 410, 757, 990, 1365, 1770
CciI TCATGA 2 cut(s) 426, 1189
CfoI GCGC 6 cut(s) 268, 340, 470, 761, 973, 2025
Cfr10I RCCGGY 1 cut(s) 2257
Cfr13I GGNCC 2 cut(s) 157, 724
CseI GACGC 1 cut(s) 1735
Csp6I GTAC 4 cut(s) 202, 2216, 2421, 2499
CviQI GTAC 4 cut(s) 202, 2216, 2421, 2499
DdeI CTNAG 5 cut(s) 236, 471, 749, 2030, 2355
DpnI GATC 4 cut(s) 431, 1635, 2187, 2253
DpnII GATC 4 cut(s) 429, 1633, 2185, 2251
EaeI YGGCCR 4 cut(s) 97, 118, 442, 547
EagI CGGCCG 1 cut(s) 442
Eam1104I CTCTTC 2 cut(s) 579, 1710
EarI CTCTTC 2 cut(s) 579, 1710
EciI GGCGGA 3 cut(s) 25, 800, 1488
EclXI CGGCCG 1 cut(s) 442
Eco105I TACGTA 1 cut(s) 1125
Eco130I CCWWGG 2 cut(s) 496, 1329
Eco24I GRGCYC 2 cut(s) 1269, 1956
Eco31I GGTCTC 2 cut(s) 139, 2358
Eco47I GGWCC 2 cut(s) 157, 724
Eco52I CGGCCG 1 cut(s) 442
Eco57I CTGAAG 2 cut(s) 1713, 1962
Eco72I CACGTG 1 cut(s) 254
Eco88I CYCGRG 1 cut(s) 167
EcoNI CCTNNNNNAGG 2 cut(s) 495, 2448
EcoO109I RGGNCCY 1 cut(s) 157
EcoRII CCWGG 3 cut(s) 273, 315, 2449
EcoT14I CCWWGG 2 cut(s) 496, 1329
EcoT22I ATGCAT 2 cut(s) 1410, 1462
EcoT38I GRGCYC 2 cut(s) 1269, 1956
ErhI CCWWGG 2 cut(s) 496, 1329
Esp3I CGTCTC 1 cut(s) 1220
FalI AAGNNNNNCTT 2 cut(s) 441, 473
FaqI GGGAC 1 cut(s) 1878
FauI CCCGC 2 cut(s) 255, 988
FauNDI CATATG 1 cut(s) 1357
FbaI TGATCA 1 cut(s) 429
FokI GGATG 7 cut(s) 5, 149, 922, 1289, 1811, 1959, 2122
FriOI GRGCYC 2 cut(s) 1269, 1956
FspBI CTAG 3 cut(s) 492, 654, 1323
FspI TGCGCA 1 cut(s) 972
GlaI GCGC 6 cut(s) 267, 339, 469, 760, 972, 2024
GsaI CCCAGC 2 cut(s) 1746, 2003
GsuI CTGGAG 2 cut(s) 2386, 2433
HaeIII GGCC 5 cut(s) 99, 120, 444, 549, 2270
HapII CCGG 6 cut(s) 96, 550, 722, 762, 827, 2258
HgaI GACGC 1 cut(s) 1735
HhaI GCGC 6 cut(s) 268, 340, 470, 761, 973, 2025
Hin1I GRCGYC 1 cut(s) 213
Hin6I GCGC 6 cut(s) 266, 338, 468, 759, 971, 2023
HinP1I GCGC 6 cut(s) 266, 338, 468, 759, 971, 2023
HindIII AAGCTT 3 cut(s) 878, 1563, 1725
HinfI GANTC 5 cut(s) 87, 914, 1895, 2338, 2368
HpaII CCGG 6 cut(s) 96, 550, 722, 762, 827, 2258
HphI GGTGA 4 cut(s) 788, 1163, 1189, 1526
Hpy166II GTNNAC 3 cut(s) 455, 1647, 2104
Hpy188I TCNGA 7 cut(s) 86, 895, 1942, 1958, 2007, 2337, 2509
Hpy8I GTNNAC 3 cut(s) 455, 1647, 2104
Hpy99I CGWCG 5 cut(s) 191, 215, 218, 485, 946
HpyAV CCTTC 9 cut(s) 91, 148, 187, 443, 880, 1229, 1405, 1937, 2111
HpyCH4III ACNGT 7 cut(s) 187, 514, 637, 1374, 1428, 2206, 2551
HpyCH4IV ACGT 7 cut(s) 213, 253, 839, 941, 1124, 1229, 1608
HpyF3I CTNAG 5 cut(s) 236, 471, 749, 2030, 2355
HpySE526I ACGT 7 cut(s) 213, 253, 839, 941, 1124, 1229, 1608
Hsp92I GRCGYC 1 cut(s) 213
HspAI GCGC 6 cut(s) 266, 338, 468, 759, 971, 2023
Kpn2I TCCGGA 1 cut(s) 826
KpnI GGTACC 1 cut(s) 2502
Ksp22I TGATCA 1 cut(s) 429
Kzo9I GATC 4 cut(s) 429, 1633, 2185, 2251
LmnI GCTCC 6 cut(s) 1075, 1108, 1272, 1705, 2303, 2405
Lsp1109I GCAGC 7 cut(s) 252, 270, 348, 394, 1379, 1431, 2144
LweI GCATC 7 cut(s) 270, 975, 1267, 1755, 1792, 1815, 2500
MaeI CTAG 3 cut(s) 492, 654, 1323
MaeII ACGT 7 cut(s) 213, 253, 839, 941, 1124, 1229, 1608
MaeIII GTNAC 5 cut(s) 181, 730, 1177, 2139, 2429
MalI GATC 4 cut(s) 431, 1635, 2187, 2253
MboI GATC 4 cut(s) 429, 1633, 2185, 2251
MboII GAAGA 9 cut(s) 274, 566, 607, 1601, 1638, 1727, 2180, 2236, 2377
MflI RGATCY 2 cut(s) 2185, 2251
MhlI GDGCHC 6 cut(s) 414, 1269, 1649, 1710, 1956, 2106
MluCI AATT 6 cut(s) 578, 869, 925, 1036, 2236, 2517
MlyI GAGTC 1 cut(s) 923
MmeI TCCRAC 3 cut(s) 645, 939, 2315
Mph1103I ATGCAT 2 cut(s) 1410, 1462
MroI TCCGGA 1 cut(s) 826
MroXI GAANNNNTTC 1 cut(s) 308
MseI TTAA 8 cut(s) 537, 642, 903, 1664, 1692, 1722, 2426, 2554
MslI CAYNNNNRTG 6 cut(s) 425, 1142, 1275, 1302, 1993, 2472
MspA1I CMGCKG 2 cut(s) 124, 385
MspCI CTTAAG 1 cut(s) 1663
MspI CCGG 6 cut(s) 96, 550, 722, 762, 827, 2258
MspR9I CCNGG 4 cut(s) 275, 317, 763, 2451
Mva1269I GAATGC 3 cut(s) 1015, 1540, 2306
MvaI CCWGG 3 cut(s) 275, 317, 2451
MvnI CGCG 6 cut(s) 266, 268, 335, 340, 447, 2025
NciI CCSGG 1 cut(s) 763
NdeI CATATG 1 cut(s) 1357
NdeII GATC 4 cut(s) 429, 1633, 2185, 2251
NlaIV GGNNCC 6 cut(s) 173, 272, 1110, 1268, 2401, 2500
NmeAIII GCCGAG 2 cut(s) 420, 429
NmuCI GTSAC 3 cut(s) 181, 1177, 2139
NsbI TGCGCA 1 cut(s) 972
NsiI ATGCAT 2 cut(s) 1410, 1462
NspI RCATGY 1 cut(s) 2299
NspV TTCGAA 1 cut(s) 1713
OliI CACNNNNGTG 1 cut(s) 1993
PagI TCATGA 2 cut(s) 426, 1189
PciI ACATGT 1 cut(s) 2295
PctI GAATGC 3 cut(s) 1015, 1540, 2306
PdmI GAANNNNTTC 1 cut(s) 308
PfeI GAWTC 4 cut(s) 87, 1895, 2338, 2368
PleI GAGTC 1 cut(s) 922
PmaCI CACGTG 1 cut(s) 254
PmlI CACGTG 1 cut(s) 254
PpsI GAGTC 1 cut(s) 922
Ppu21I YACGTR 3 cut(s) 254, 840, 1125
PpuMI RGGWCCY 1 cut(s) 157
PscI ACATGT 1 cut(s) 2295
PshBI ATTAAT 1 cut(s) 537
PsiI TTATAA 2 cut(s) 630, 738
Psp5II RGGWCCY 1 cut(s) 157
Psp6I CCWGG 3 cut(s) 273, 315, 2449
PspCI CACGTG 1 cut(s) 254
PspFI CCCAGC 2 cut(s) 1742, 1999
PspGI CCWGG 3 cut(s) 273, 315, 2449
PspN4I GGNNCC 6 cut(s) 173, 272, 1110, 1268, 2401, 2500
PspPI GGNCC 2 cut(s) 157, 724
PspPPI RGGWCCY 1 cut(s) 157
PsrI GAACNNNNNNTAC 2 cut(s) 2153, 2185
PstI CTGCAG 1 cut(s) 2289
PsuI RGATCY 2 cut(s) 2185, 2251
RsaI GTAC 4 cut(s) 203, 2217, 2422, 2500
RsaNI GTAC 4 cut(s) 202, 2216, 2421, 2499
RseI CAYNNNNRTG 6 cut(s) 425, 1142, 1275, 1302, 1993, 2472
SaqAI TTAA 8 cut(s) 537, 642, 903, 1664, 1692, 1722, 2426, 2554
Sau3AI GATC 4 cut(s) 429, 1633, 2185, 2251
Sau96I GGNCC 2 cut(s) 157, 724
SbfI CCTGCAGG 1 cut(s) 2289
SchI GAGTC 1 cut(s) 923
ScrFI CCNGG 4 cut(s) 275, 317, 763, 2451
SdaI CCTGCAGG 1 cut(s) 2289
SduI GDGCHC 6 cut(s) 414, 1269, 1649, 1710, 1956, 2106
SfaNI GCATC 7 cut(s) 270, 975, 1267, 1755, 1792, 1815, 2500
SfcI CTRYAG 3 cut(s) 2094, 2285, 2391
SfuI TTCGAA 1 cut(s) 1713
SinI GGWCC 2 cut(s) 157, 724
SmiMI CAYNNNNRTG 6 cut(s) 425, 1142, 1275, 1302, 1993, 2472
SmlI CTYRAG 5 cut(s) 47, 881, 1085, 1663, 2360
SmoI CTYRAG 5 cut(s) 47, 881, 1085, 1663, 2360
SnaBI TACGTA 1 cut(s) 1125
Sse8387I CCTGCAGG 1 cut(s) 2289
Sse9I AATT 6 cut(s) 578, 869, 925, 1036, 2236, 2517
SspI AATATT 3 cut(s) 1132, 1210, 1849
SspMI CTAG 3 cut(s) 492, 654, 1323
StyD4I CCNGG 4 cut(s) 273, 315, 761, 2449
StyI CCWWGG 2 cut(s) 496, 1329
TaaI ACNGT 7 cut(s) 187, 514, 637, 1374, 1428, 2206, 2551
TaiI ACGT 7 cut(s) 216, 256, 842, 944, 1127, 1232, 1611
TaqI TCGA 6 cut(s) 189, 216, 480, 1505, 1713, 2375
TaqII GACCGA 1 cut(s) 121
TasI AATT 6 cut(s) 578, 869, 925, 1036, 2236, 2517
TatI WGTACW 2 cut(s) 201, 2420
TauI GCSGC 7 cut(s) 338, 367, 388, 447, 995, 1016, 2273
TfiI GAWTC 4 cut(s) 87, 1895, 2338, 2368
Tru1I TTAA 8 cut(s) 537, 642, 903, 1664, 1692, 1722, 2426, 2554
Tru9I TTAA 8 cut(s) 537, 642, 903, 1664, 1692, 1722, 2426, 2554
TscAI CASTG 2 cut(s) 532, 1377
TseFI GTSAC 3 cut(s) 181, 1177, 2139
TseI GCWGC 7 cut(s) 240, 258, 361, 382, 1367, 1419, 2132
Tsp45I GTSAC 3 cut(s) 181, 1177, 2139
TspGWI ACGGA 4 cut(s) 127, 169, 1121, 2060
TspRI CASTG 2 cut(s) 532, 1377
Vha464I CTTAAG 1 cut(s) 1663
VneI GTGCAC 2 cut(s) 1645, 2102
VpaK11BI GGWCC 2 cut(s) 157, 724
VspI ATTAAT 1 cut(s) 537
XagI CCTNNNNNAGG 2 cut(s) 495, 2448
XapI RAATTY 3 cut(s) 925, 1036, 2236
XceI RCATGY 1 cut(s) 2299
XmnI GAANNNNTTC 1 cut(s) 308
XspI CTAG 3 cut(s) 492, 654, 1323
ZraI GACGTC 1 cut(s) 214
Zsp2I ATGCAT 2 cut(s) 1410, 1462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.