RLG00000005307

Pentatricopeptide repeat-containing protein At1g74850

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0009497
Physical Location & Seq
Reverse (-)
66693897 .. 66697992
4096 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005307

Sequence Viewer

Length: 2706 bp
ATGACCCTCTCCATTTCCATCCCTTCCCCTCTCTCCGCCCCAATCCTCAAGCTAAACCCTAAACCCCACCACCTCTCCTCCTTCCGATTCCTCTCCGGCCACCGCAAGTTCCTCCACGGCCAGCGGTTATCGGTCTCCGTTAGAGCAAAGCCGAAGGACCTCATCCTCGGGAACCCGTCCGTCACCGTCGAGAAGGGCAAGTACAGCTACGACGTCGAAACCCTAATCAACAAGCTCAGCAGCCTCCCGCCACGTGGCAGCATCGCGCGGTGCCTGGACATCTTCAAGAACAAGCTCTCCCTCAACGACTTCGCCCTGGTGTTCAAGGAGTTCGCGGCGCGTGGCGATTGGCAGAGATCGCTGCGGCTGTTCAAGTACATGCAGCGGCAGATATGGTGCAAGCCGAGCGAGCACATCTACACCATCATGATCAGTCTGCTCGGCCGCGAAGGTTTGCTTGAGAAATGCGCTGAGATTTTCGACGAAATGCCTAGCCAAGGTGTGATTAGGACTGTGTTTAGCTACACTGCTTTGATAAATGCCTATGGCCGGAATGGTCAGTATGAAATGTCTCTTCAATTACTTGATAGAATGAAGAAAGATAGGGTTTCGCCGAATATATTGACTTATAATACTGTGCTTAATGCTTGTGCTAGAGGGGGGTTGGATTGGGAGGGGTTGTTAGGATTGTTTGCCGAAATGAGGCACGAGGGGGTTCAGCCGGACCTTGTTACTTATAATACATTGCTTAGTGCTTGCGCCGGGAGAGGTTTAGGTGATGAGGCGGAGATGGTGTTTAGGACTATGAATGAGGGTGGCATAGTTCCGGATATAACCACGTATAGTTATCTTGTTGAGACTTTTGGTAAATTGGATAAGCTTGAGAAGGTTTCCGAGTTGCTTAAGGGGATGGAGTCCGGGGGGAATTTGCCTGATATTACGTCGTATAATGTGTTGTTGGAGGCGTATGCGCAGTTGGGGTCGATTAAGGAGGCAATGGGTGTGTTTAGGCAGATGCAAGCGGCGGGGTGTATGCCGAATGCGGCTACTTATAGTATTTTGTTGAATTTGTATGGGAGGCATGGGAGGTATGATGATGTTCGGGAGCTTTTTCTTGAGATGAAAGTGAGCAATACGGAGCCAGATGCTGCTACGTATAATATTCTCATTCAGGTGTTTGGTGAGGGTGGGTATTTTCGGGAGGTGGTCACTTTGTTTCATGACATGGTAGAGGAGAATATTGAGCCGAATATGGAGACGTATGAAGGATTGATATATGCTTGTGGAAAAGGAGGGCTCCATGAGGATGCGAAGAACATGTTGCTTCACATGAATGAGAAGGGAATAGTGCCTAGTTCCAAGGCTTATACAGGAGTTATTGAAGCATATGGGCAAGCAGCACTGTATGACGAAGCTCTTGTTGCCTTCAACACAATGCATGAAGTGGGCAGCAGACCGTCAGTTGAAAGCTACAACTCGCTTATTCATGCATATGCAAGAGGTGGACTGTACAAGGAGACTGAACAAATCTTATCGATAATGGGTGAGGTTGGCATTGCAAGGAATGCCAGTTCATTCAATGGAATGATTGAAGCTTTTAGGCAGGGAGGTCAGTTTGAAGAAGCTATAAAGACATACGTTGAGATGGAAAAGAGAAGATGTGATCCCGATGAGTGCACTCTTGAAGCAGTCTTAAGCGTTTATAGTGTTGCAGGTCTTGTTAGTGAGTGTGAGGAGCACTTCGAAGAGATTAAAGCTTCAGGGATATTGCCCAGCGTCATGTGCTACTGCATGATGCTTGCTGTTTATGCAAAGACTAACAGGTGGGATGATGCCAATAAGTTGCTGAATGAGATGCTCACAAATAGGGTATCAAATATTCATCAAGTGATGGGACAGATGATGAAGGGGGATTATGATGACGAATCTAACTGGCAGATGGTAGAGTATGTTTTTGACAAACTAAAATCTGAAGGATGTGGGCTCGGAATGAGGTTCTACAACACTTTGATAGAAGCACTTTGGTGGCTGGGTCAGAAACAAAGAGCTGTGCGAGTTCTTAGTGAAGCAACTCAACGGGGGCTGTTTCCTGAACTTCTCCGTAAAAATAAACTTGTTTGGTCTATAGATGTGCACAGGATGTGGGAAGGTGGTGCATATGCAGCAATGTCACTTTGGCTAAACAATATGTATGAGATGTTCCTGAATGGCGAGGATCTTCCCTATGTTGCAACTGTTGTTGTAGTACGGGGCAAGATGGAAAAAGGTTCAACAACACAAGATCTGCCGGTTGCAAAGGCCGCCTATTCATTCCTGCAGGATAACATGTCAGGAGCATTCAGTTTCCCAAAATGGAACAATGGTCGGATTCTCTGCCAAAGGTCTCAGCTCAAGAAGATTCTGTCAAGCATTGAACCATCTACGGACGGCTCCAGCAGTAAAAGTATATGTACTTTAAGTAACTCCCCTTTTCCTCCTCCAGGCACAAAAACATCACCCACTGATGTTGATAGTGTTCTGTATAATGGTACCACTTCTGATGCAACAAGTTGGACAAGAACAGAGCTTTTGACGAGCACACCAGCTGAAGTTTTAGCACGGCAAGATGGCAGCATTGACCAAACTGCGAATACTATGCGCAAGACAAGTTTTGATCCTGGATTACCTTTGAAAGACCCAGAGGTGACGAGGATTCTAGTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

902

Amino Acids

100.85

Weight (kDa)

5.71

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 135 - 179 1.1e-06 PPR repeat family
PPR PF01535 139 - 168 4.2e-06 PPR repeat
PPR_3 PF13812 159 - 217 7e-15 Pentatricopeptide repeat domain
PPR_2 PF13041 171 - 219 3e-14 PPR repeat family
PPR PF01535 173 - 202 1.2e-07 PPR repeat
PPR_long PF17177 191 - 328 1.2e-10 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 213 - 339 4.7e-09 Fungal tetratrico peptide repeats
PPR_2 PF13041 228 - 254 4.4e-06 PPR repeat family
PPR_3 PF13812 230 - 290 7.4e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 238 - 269 7.6e-06 PPR repeat
PPR_2 PF13041 241 - 288 2.9e-11 PPR repeat family
PPR_3 PF13812 300 - 359 1.7e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 311 - 356 6e-13 PPR repeat family
PPR PF01535 315 - 344 3.4e-06 PPR repeat
TPR_24 PF23276 315 - 407 2.1e-07 Fungal tetratrico peptide repeats
PPR PF01535 350 - 377 6.4e-06 PPR repeat
PPR_2 PF13041 353 - 393 2.6e-09 PPR repeat family
PPR_2 PF13041 381 - 430 1.6e-10 PPR repeat family
PPR_3 PF13812 405 - 465 5.7e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 451 - 500 4e-06 PPR repeat family
PPR_3 PF13812 478 - 533 4.2e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 522 - 565 2.3e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 630, 738
AatII GACGTC 1 cut(s) 216
Acc16I TGCGCA 2 cut(s) 972, 2639
Acc36I ACCTGC 1 cut(s) 1703
Acc65I GGTACC 1 cut(s) 2528
AccB1I GGYRCC 2 cut(s) 270, 2528
AccII CGCG 5 cut(s) 266, 268, 335, 340, 447
AccIII TCCGGA 1 cut(s) 826
AclWI GGATC 3 cut(s) 1658, 2223, 2648
AcoI YGGCCR 4 cut(s) 97, 118, 442, 547
AcsI RAATTY 2 cut(s) 925, 1066
AcuI CTGAAG 3 cut(s) 1743, 1992, 2607
AcvI CACGTG 1 cut(s) 254
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 6 cut(s) 203, 377, 1511, 2247, 2452, 2530
AfiI CCNNNNNNNGG 5 cut(s) 254, 497, 549, 702, 2480
AflII CTTAAG 2 cut(s) 902, 1693
AflIII ACRYGT 2 cut(s) 1317, 2325
AjnI CCWGG 4 cut(s) 273, 315, 2479, 2656
AleI CACNNNNGTG 1 cut(s) 2023
AloI GAACNNNNNNTCC 4 cut(s) 281, 313, 1555, 1587
Alw21I GWGCWC 5 cut(s) 414, 1679, 1740, 2136, 2579
Alw26I GTCTC 6 cut(s) 139, 576, 851, 1250, 1511, 2388
Alw44I GTGCAC 2 cut(s) 1675, 2132
AlwI GGATC 3 cut(s) 1658, 2223, 2648
AlwNI CAGNNNCTG 1 cut(s) 1148
Ama87I CYCGRG 1 cut(s) 167
Aor13HI TCCGGA 1 cut(s) 826
AoxI GGCC 5 cut(s) 97, 118, 442, 547, 2298
ApaLI GTGCAC 2 cut(s) 1675, 2132
ApeKI GCWGC 9 cut(s) 240, 258, 361, 382, 1148, 1397, 1449, 2162, 2610
ApoI RAATTY 2 cut(s) 925, 1066
Asp718I GGTACC 1 cut(s) 2528
AspLEI GCGC 6 cut(s) 268, 340, 470, 761, 973, 2640
AspS9I GGNCC 2 cut(s) 157, 724
AsuC2I CCSGG 2 cut(s) 763, 919
AsuHPI GGTGA 6 cut(s) 175, 788, 1193, 1556, 2487, 2695
AsuII TTCGAA 1 cut(s) 1743
AvaI CYCGRG 1 cut(s) 167
AvaII GGWCC 2 cut(s) 157, 724
BaeGI GKGCMC 2 cut(s) 1679, 2136
BanI GGYRCC 2 cut(s) 270, 2528
BanII GRGCYC 2 cut(s) 1299, 1986
BarI GAAGNNNNNNTAC 2 cut(s) 185, 217
BauI CACGAG 1 cut(s) 707
BbrPI CACGTG 1 cut(s) 254
Bbv12I GWGCWC 5 cut(s) 414, 1679, 1740, 2136, 2579
BbvI GCAGC 9 cut(s) 252, 270, 348, 394, 1135, 1409, 1461, 2174, 2622
BceAI ACGGC 3 cut(s) 133, 2443, 2615
BcgI CGANNNNNNTGC 2 cut(s) 2617, 2651
BciT130I CCWGG 4 cut(s) 275, 317, 2481, 2658
BclI TGATCA 1 cut(s) 429
BcnI CCSGG 2 cut(s) 763, 919
BcoDI GTCTC 6 cut(s) 139, 576, 851, 1250, 1511, 2388
BfaI CTAG 4 cut(s) 492, 654, 1353, 2696
BfmI CTRYAG 2 cut(s) 2124, 2315
BfrI CTTAAG 2 cut(s) 902, 1693
BfuAI ACCTGC 1 cut(s) 1703
BglII AGATCT 1 cut(s) 2281
BlpI GCTNAGC 1 cut(s) 236
Bme1390I CCNGG 6 cut(s) 275, 317, 763, 919, 2481, 2658
Bme18I GGWCC 2 cut(s) 157, 724
BmeT110I CYCGRG 1 cut(s) 167
BmgT120I GGNCC 2 cut(s) 157, 724
BmiI GGNNCC 6 cut(s) 173, 272, 1140, 1298, 2431, 2530
BmrFI CCNGG 6 cut(s) 275, 317, 763, 919, 2481, 2658
BmsI GCATC 8 cut(s) 270, 1005, 1135, 1297, 1785, 1822, 1845, 2530
BpmI CTGGAG 2 cut(s) 2416, 2463
Bpu1102I GCTNAGC 1 cut(s) 236
Bpu14I TTCGAA 1 cut(s) 1743
BpuEI CTTGAG 5 cut(s) 32, 479, 902, 1136, 2375
BpuMI CCSGG 2 cut(s) 763, 919
Bsa29I ATCGAT 1 cut(s) 1535
BsaAI YACGTR 3 cut(s) 254, 840, 1155
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 2 cut(s) 139, 2388
BsaJI CCNNGG 6 cut(s) 115, 166, 315, 496, 918, 1359
BsaWI WCCGGW 1 cut(s) 826
Bsc4I CCNNNNNNNGG 5 cut(s) 254, 497, 549, 702, 2480
Bse118I RCCGGY 1 cut(s) 2287
Bse1I ACTGG 2 cut(s) 1569, 1937
Bse3DI GCAATG 4 cut(s) 743, 1002, 1554, 2172
BseAI TCCGGA 1 cut(s) 826
BseBI CCWGG 4 cut(s) 275, 317, 2481, 2658
BseCI ATCGAT 1 cut(s) 1535
BseDI CCNNGG 6 cut(s) 115, 166, 315, 496, 918, 1359
BseGI GGATG 7 cut(s) 18, 162, 915, 1312, 1834, 1982, 2145
BseLI CCNNNNNNNGG 5 cut(s) 254, 497, 549, 702, 2480
BseMI GCAATG 4 cut(s) 743, 1002, 1554, 2172
BseMII CTCAG 3 cut(s) 250, 462, 2399
BseNI ACTGG 2 cut(s) 1569, 1937
BseRI GAGGAG 4 cut(s) 67, 1247, 1748, 2466
BseSI GKGCMC 2 cut(s) 1679, 2136
BseX3I CGGCCG 1 cut(s) 442
BseXI GCAGC 9 cut(s) 252, 270, 348, 394, 1135, 1409, 1461, 2174, 2622
BseYI CCCAGC 2 cut(s) 1772, 2029
Bsh1236I CGCG 5 cut(s) 266, 268, 335, 340, 447
Bsh1285I CGRYCG 1 cut(s) 445
BshFI GGCC 5 cut(s) 99, 120, 444, 549, 2300
BshNI GGYRCC 2 cut(s) 270, 2528
BshVI ATCGAT 1 cut(s) 1535
BsiEI CGRYCG 1 cut(s) 445
BsiHKAI GWGCWC 5 cut(s) 414, 1679, 1740, 2136, 2579
BsiHKCI CYCGRG 1 cut(s) 167
BsiSI CCGG 7 cut(s) 96, 550, 722, 762, 827, 918, 2288
BslFI GGGAC 1 cut(s) 1908
BslI CCNNNNNNNGG 5 cut(s) 254, 497, 549, 702, 2480
BsmAI GTCTC 6 cut(s) 139, 576, 851, 1250, 1511, 2388
BsmBI CGTCTC 1 cut(s) 1250
BsmFI GGGAC 1 cut(s) 1908
BsmI GAATGC 3 cut(s) 1045, 1570, 2336
BsnI GGCC 5 cut(s) 99, 120, 444, 549, 2300
Bso31I GGTCTC 2 cut(s) 139, 2388
BsoBI CYCGRG 1 cut(s) 167
Bsp119I TTCGAA 1 cut(s) 1743
Bsp1286I GDGCHC 7 cut(s) 414, 1299, 1679, 1740, 1986, 2136, 2579
Bsp13I TCCGGA 1 cut(s) 826
Bsp1407I TGTACA 1 cut(s) 1509
Bsp143I GATC 6 cut(s) 356, 429, 1663, 2215, 2281, 2653
Bsp1720I GCTNAGC 1 cut(s) 236
BspANI GGCC 5 cut(s) 99, 120, 444, 549, 2300
BspCNI CTCAG 3 cut(s) 249, 463, 2398
BspDI ATCGAT 1 cut(s) 1535
BspEI TCCGGA 1 cut(s) 826
BspFNI CGCG 5 cut(s) 266, 268, 335, 340, 447
BspHI TCATGA 2 cut(s) 426, 1219
BspLI GGNNCC 6 cut(s) 173, 272, 1140, 1298, 2431, 2530
BspMAI CTGCAG 1 cut(s) 2319
BspMI ACCTGC 1 cut(s) 1703
BspPI GGATC 3 cut(s) 1658, 2223, 2648
BspT104I TTCGAA 1 cut(s) 1743
BspT107I GGYRCC 2 cut(s) 270, 2528
BspTI CTTAAG 2 cut(s) 902, 1693
BspTNI GGTCTC 2 cut(s) 139, 2388
BsrDI GCAATG 4 cut(s) 743, 1002, 1554, 2172
BsrFI RCCGGY 1 cut(s) 2287
BsrGI TGTACA 1 cut(s) 1509
BsrI ACTGG 2 cut(s) 1569, 1937
BssAI RCCGGY 1 cut(s) 2287
BssECI CCNNGG 6 cut(s) 115, 166, 315, 496, 918, 1359
BssMI GATC 6 cut(s) 356, 429, 1663, 2215, 2281, 2653
BssNI GRCGYC 1 cut(s) 213
BssSI CACGAG 1 cut(s) 707
BssT1I CCWWGG 2 cut(s) 496, 1359
Bst2BI CACGAG 1 cut(s) 707
Bst2UI CCWGG 4 cut(s) 275, 317, 2481, 2658
Bst4CI ACNGT 7 cut(s) 187, 514, 637, 1404, 1458, 1509, 2236
Bst6I CTCTTC 2 cut(s) 579, 1740
BstACI GRCGYC 1 cut(s) 213
BstAFI CTTAAG 2 cut(s) 902, 1693
BstAPI GCANNNNNTGC 1 cut(s) 1565
BstAUI TGTACA 1 cut(s) 1509
BstBAI YACGTR 3 cut(s) 254, 840, 1155
BstBI TTCGAA 1 cut(s) 1743
BstC8I GCNNGC 7 cut(s) 122, 401, 410, 757, 1020, 1395, 1800
BstDEI CTNAG 5 cut(s) 236, 471, 749, 2060, 2385
BstDSI CCRYGG 1 cut(s) 115
BstENI CCTNNNNNAGG 2 cut(s) 495, 2478
BstF5I GGATG 7 cut(s) 18, 162, 915, 1312, 1834, 1982, 2145
BstFNI CGCG 5 cut(s) 266, 268, 335, 340, 447
BstHHI GCGC 6 cut(s) 268, 340, 470, 761, 973, 2640
BstKTI GATC 6 cut(s) 359, 432, 1666, 2218, 2284, 2656
BstMAI GTCTC 6 cut(s) 139, 576, 851, 1250, 1511, 2388
BstMBI GATC 6 cut(s) 356, 429, 1663, 2215, 2281, 2653
BstMCI CGRYCG 1 cut(s) 445
BstNI CCWGG 4 cut(s) 275, 317, 2481, 2658
BstNSI RCATGY 3 cut(s) 382, 1321, 2329
BstSCI CCNGG 6 cut(s) 273, 315, 761, 917, 2479, 2656
BstSFI CTRYAG 2 cut(s) 2124, 2315
BstSLI GKGCMC 2 cut(s) 1679, 2136
BstSNI TACGTA 1 cut(s) 1155
BstUI CGCG 5 cut(s) 266, 268, 335, 340, 447
BstV1I GCAGC 9 cut(s) 252, 270, 348, 394, 1135, 1409, 1461, 2174, 2622
BstX2I RGATCY 2 cut(s) 2215, 2281
BstYI RGATCY 2 cut(s) 2215, 2281
BstZI CGGCCG 1 cut(s) 442
Bsu15I ATCGAT 1 cut(s) 1535
BsuRI GGCC 5 cut(s) 99, 120, 444, 549, 2300
BsuTUI ATCGAT 1 cut(s) 1535
BtgI CCRYGG 1 cut(s) 115
BtgZI GCGATG 1 cut(s) 247
BtsCI GGATG 7 cut(s) 18, 162, 915, 1312, 1834, 1982, 2145
BtsI GCAGTG 1 cut(s) 525
BtsIMutI CAGTG 3 cut(s) 525, 1400, 2499
BveI ACCTGC 1 cut(s) 1703
Cac8I GCNNGC 7 cut(s) 122, 401, 410, 757, 1020, 1395, 1800
CaiI CAGNNNCTG 1 cut(s) 1148
CciI TCATGA 2 cut(s) 426, 1219
CfoI GCGC 6 cut(s) 268, 340, 470, 761, 973, 2640
Cfr10I RCCGGY 1 cut(s) 2287
Cfr13I GGNCC 2 cut(s) 157, 724
ClaI ATCGAT 1 cut(s) 1535
CseI GACGC 1 cut(s) 1765
Csp6I GTAC 6 cut(s) 202, 376, 1510, 2246, 2451, 2529
CspCI CAANNNNNGTGG 2 cut(s) 2488, 2523
CviQI GTAC 6 cut(s) 202, 376, 1510, 2246, 2451, 2529
DdeI CTNAG 5 cut(s) 236, 471, 749, 2060, 2385
DpnI GATC 6 cut(s) 358, 431, 1665, 2217, 2283, 2655
DpnII GATC 6 cut(s) 356, 429, 1663, 2215, 2281, 2653
EaeI YGGCCR 4 cut(s) 97, 118, 442, 547
EagI CGGCCG 1 cut(s) 442
Eam1104I CTCTTC 2 cut(s) 579, 1740
EarI CTCTTC 2 cut(s) 579, 1740
EciI GGCGGA 2 cut(s) 25, 800
EclXI CGGCCG 1 cut(s) 442
Eco105I TACGTA 1 cut(s) 1155
Eco130I CCWWGG 2 cut(s) 496, 1359
Eco24I GRGCYC 2 cut(s) 1299, 1986
Eco31I GGTCTC 2 cut(s) 139, 2388
Eco47I GGWCC 2 cut(s) 157, 724
Eco52I CGGCCG 1 cut(s) 442
Eco57I CTGAAG 3 cut(s) 1743, 1992, 2607
Eco72I CACGTG 1 cut(s) 254
Eco88I CYCGRG 1 cut(s) 167
EcoNI CCTNNNNNAGG 2 cut(s) 495, 2478
EcoO109I RGGNCCY 1 cut(s) 157
EcoRII CCWGG 4 cut(s) 273, 315, 2479, 2656
EcoT14I CCWWGG 2 cut(s) 496, 1359
EcoT22I ATGCAT 2 cut(s) 1440, 1492
EcoT38I GRGCYC 2 cut(s) 1299, 1986
ErhI CCWWGG 2 cut(s) 496, 1359
Esp3I CGTCTC 1 cut(s) 1250
FalI AAGNNNNNCTT 2 cut(s) 441, 473
FaqI GGGAC 1 cut(s) 1908
FauI CCCGC 2 cut(s) 255, 1018
FauNDI CATATG 3 cut(s) 1387, 1492, 2158
FbaI TGATCA 1 cut(s) 429
FokI GGATG 7 cut(s) 5, 149, 922, 1319, 1841, 1989, 2152
FriOI GRGCYC 2 cut(s) 1299, 1986
FspBI CTAG 4 cut(s) 492, 654, 1353, 2696
FspI TGCGCA 2 cut(s) 972, 2639
GlaI GCGC 6 cut(s) 267, 339, 469, 760, 972, 2639
GsaI CCCAGC 2 cut(s) 1776, 2033
GsuI CTGGAG 2 cut(s) 2416, 2463
HaeIII GGCC 5 cut(s) 99, 120, 444, 549, 2300
HapII CCGG 7 cut(s) 96, 550, 722, 762, 827, 918, 2288
HgaI GACGC 1 cut(s) 1765
HhaI GCGC 6 cut(s) 268, 340, 470, 761, 973, 2640
Hin1I GRCGYC 1 cut(s) 213
Hin6I GCGC 6 cut(s) 266, 338, 468, 759, 971, 2638
HinP1I GCGC 6 cut(s) 266, 338, 468, 759, 971, 2638
HindIII AAGCTT 3 cut(s) 878, 1593, 1755
HinfI GANTC 6 cut(s) 87, 914, 1925, 2368, 2398, 2692
HpaII CCGG 7 cut(s) 96, 550, 722, 762, 827, 918, 2288
HphI GGTGA 6 cut(s) 175, 788, 1193, 1556, 2487, 2695
Hpy166II GTNNAC 3 cut(s) 1505, 1677, 2134
Hpy188I TCNGA 7 cut(s) 86, 895, 1972, 1988, 2037, 2367, 2539
Hpy8I GTNNAC 3 cut(s) 1505, 1677, 2134
Hpy99I CGWCG 5 cut(s) 191, 215, 218, 485, 946
HpyCH4III ACNGT 7 cut(s) 187, 514, 637, 1404, 1458, 1509, 2236
HpyCH4IV ACGT 7 cut(s) 213, 253, 839, 941, 1154, 1259, 1638
HpyF3I CTNAG 5 cut(s) 236, 471, 749, 2060, 2385
HpySE526I ACGT 7 cut(s) 213, 253, 839, 941, 1154, 1259, 1638
Hsp92I GRCGYC 1 cut(s) 213
HspAI GCGC 6 cut(s) 266, 338, 468, 759, 971, 2638
Kpn2I TCCGGA 1 cut(s) 826
KpnI GGTACC 1 cut(s) 2532
Ksp22I TGATCA 1 cut(s) 429
Kzo9I GATC 6 cut(s) 356, 429, 1663, 2215, 2281, 2653
LmnI GCTCC 6 cut(s) 1105, 1138, 1302, 1735, 2333, 2435
Lsp1109I GCAGC 9 cut(s) 252, 270, 348, 394, 1135, 1409, 1461, 2174, 2622
LweI GCATC 8 cut(s) 270, 1005, 1135, 1297, 1785, 1822, 1845, 2530
MaeI CTAG 4 cut(s) 492, 654, 1353, 2696
MaeII ACGT 7 cut(s) 213, 253, 839, 941, 1154, 1259, 1638
MaeIII GTNAC 6 cut(s) 181, 730, 1207, 2169, 2459, 2683
MalI GATC 6 cut(s) 358, 431, 1665, 2217, 2283, 2655
MboI GATC 6 cut(s) 356, 429, 1663, 2215, 2281, 2653
MboII GAAGA 9 cut(s) 274, 566, 607, 1324, 1631, 1668, 1757, 2210, 2407
MflI RGATCY 2 cut(s) 2215, 2281
MhlI GDGCHC 7 cut(s) 414, 1299, 1679, 1740, 1986, 2136, 2579
MluCI AATT 4 cut(s) 578, 869, 925, 1066
MlyI GAGTC 1 cut(s) 923
MmeI TCCRAC 4 cut(s) 645, 939, 2345, 2531
Mph1103I ATGCAT 2 cut(s) 1440, 1492
MroI TCCGGA 1 cut(s) 826
MseI TTAA 6 cut(s) 642, 903, 987, 1694, 1752, 2456
MslI CAYNNNNRTG 6 cut(s) 425, 1172, 1305, 1332, 1491, 2023
MspA1I CMGCKG 3 cut(s) 124, 385, 2585
MspCI CTTAAG 2 cut(s) 902, 1693
MspI CCGG 7 cut(s) 96, 550, 722, 762, 827, 918, 2288
MspR9I CCNGG 6 cut(s) 275, 317, 763, 919, 2481, 2658
Mva1269I GAATGC 3 cut(s) 1045, 1570, 2336
MvaI CCWGG 4 cut(s) 275, 317, 2481, 2658
MvnI CGCG 5 cut(s) 266, 268, 335, 340, 447
NciI CCSGG 2 cut(s) 763, 919
NdeI CATATG 3 cut(s) 1387, 1492, 2158
NdeII GATC 6 cut(s) 356, 429, 1663, 2215, 2281, 2653
NlaIV GGNNCC 6 cut(s) 173, 272, 1140, 1298, 2431, 2530
NmeAIII GCCGAG 2 cut(s) 420, 429
NmuCI GTSAC 4 cut(s) 181, 1207, 2169, 2683
NsbI TGCGCA 2 cut(s) 972, 2639
NsiI ATGCAT 2 cut(s) 1440, 1492
NspI RCATGY 3 cut(s) 382, 1321, 2329
NspV TTCGAA 1 cut(s) 1743
OliI CACNNNNGTG 1 cut(s) 2023
PagI TCATGA 2 cut(s) 426, 1219
PciI ACATGT 2 cut(s) 1317, 2325
PctI GAATGC 3 cut(s) 1045, 1570, 2336
PfeI GAWTC 5 cut(s) 87, 1925, 2368, 2398, 2692
PfoI TCCNGGA 1 cut(s) 2656
PleI GAGTC 1 cut(s) 922
PmaCI CACGTG 1 cut(s) 254
PmlI CACGTG 1 cut(s) 254
PpsI GAGTC 1 cut(s) 922
Ppu21I YACGTR 3 cut(s) 254, 840, 1155
PpuMI RGGWCCY 1 cut(s) 157
PscI ACATGT 2 cut(s) 1317, 2325
PsiI TTATAA 2 cut(s) 630, 738
Psp5II RGGWCCY 1 cut(s) 157
Psp6I CCWGG 4 cut(s) 273, 315, 2479, 2656
PspCI CACGTG 1 cut(s) 254
PspFI CCCAGC 2 cut(s) 1772, 2029
PspGI CCWGG 4 cut(s) 273, 315, 2479, 2656
PspN4I GGNNCC 6 cut(s) 173, 272, 1140, 1298, 2431, 2530
PspPI GGNCC 2 cut(s) 157, 724
PspPPI RGGWCCY 1 cut(s) 157
PsrI GAACNNNNNNTAC 2 cut(s) 2183, 2215
PstI CTGCAG 1 cut(s) 2319
PstNI CAGNNNCTG 1 cut(s) 1148
PsuI RGATCY 2 cut(s) 2215, 2281
PvuII CAGCTG 1 cut(s) 2585
RsaI GTAC 6 cut(s) 203, 377, 1511, 2247, 2452, 2530
RsaNI GTAC 6 cut(s) 202, 376, 1510, 2246, 2451, 2529
RseI CAYNNNNRTG 6 cut(s) 425, 1172, 1305, 1332, 1491, 2023
SaqAI TTAA 6 cut(s) 642, 903, 987, 1694, 1752, 2456
Sau3AI GATC 6 cut(s) 356, 429, 1663, 2215, 2281, 2653
Sau96I GGNCC 2 cut(s) 157, 724
SbfI CCTGCAGG 1 cut(s) 2319
SchI GAGTC 1 cut(s) 923
ScrFI CCNGG 6 cut(s) 275, 317, 763, 919, 2481, 2658
SdaI CCTGCAGG 1 cut(s) 2319
SduI GDGCHC 7 cut(s) 414, 1299, 1679, 1740, 1986, 2136, 2579
SfaNI GCATC 8 cut(s) 270, 1005, 1135, 1297, 1785, 1822, 1845, 2530
SfcI CTRYAG 2 cut(s) 2124, 2315
SfuI TTCGAA 1 cut(s) 1743
SinI GGWCC 2 cut(s) 157, 724
SmiMI CAYNNNNRTG 6 cut(s) 425, 1172, 1305, 1332, 1491, 2023
SmlI CTYRAG 7 cut(s) 47, 458, 881, 902, 1115, 1693, 2390
SmoI CTYRAG 7 cut(s) 47, 458, 881, 902, 1115, 1693, 2390
SnaBI TACGTA 1 cut(s) 1155
Sse8387I CCTGCAGG 1 cut(s) 2319
Sse9I AATT 4 cut(s) 578, 869, 925, 1066
SspI AATATT 3 cut(s) 1162, 1240, 1879
SspMI CTAG 4 cut(s) 492, 654, 1353, 2696
StyD4I CCNGG 6 cut(s) 273, 315, 761, 917, 2479, 2656
StyI CCWWGG 2 cut(s) 496, 1359
TaaI ACNGT 7 cut(s) 187, 514, 637, 1404, 1458, 1509, 2236
TaiI ACGT 7 cut(s) 216, 256, 842, 944, 1157, 1262, 1641
TaqI TCGA 6 cut(s) 189, 216, 480, 983, 1535, 1743
TaqII GACCGA 1 cut(s) 121
TasI AATT 4 cut(s) 578, 869, 925, 1066
TatI WGTACW 4 cut(s) 201, 375, 1509, 2450
TauI GCSGC 7 cut(s) 338, 367, 388, 447, 1025, 1046, 2303
TfiI GAWTC 5 cut(s) 87, 1925, 2368, 2398, 2692
Tru1I TTAA 6 cut(s) 642, 903, 987, 1694, 1752, 2456
Tru9I TTAA 6 cut(s) 642, 903, 987, 1694, 1752, 2456
TscAI CASTG 3 cut(s) 532, 1407, 2506
TseFI GTSAC 4 cut(s) 181, 1207, 2169, 2683
TseI GCWGC 9 cut(s) 240, 258, 361, 382, 1148, 1397, 1449, 2162, 2610
Tsp45I GTSAC 4 cut(s) 181, 1207, 2169, 2683
TspGWI ACGGA 5 cut(s) 127, 169, 1151, 2090, 2438
TspRI CASTG 3 cut(s) 532, 1407, 2506
Vha464I CTTAAG 2 cut(s) 902, 1693
VneI GTGCAC 2 cut(s) 1675, 2132
VpaK11BI GGWCC 2 cut(s) 157, 724
XagI CCTNNNNNAGG 2 cut(s) 495, 2478
XapI RAATTY 2 cut(s) 925, 1066
XceI RCATGY 3 cut(s) 382, 1321, 2329
XspI CTAG 4 cut(s) 492, 654, 1353, 2696
ZraI GACGTC 1 cut(s) 214
Zsp2I ATGCAT 2 cut(s) 1440, 1492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.