Rroxscaffold_3G00273280

Pentatricopeptide repeat-containing protein At1g74850

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009497
Physical Location & Seq
Reverse (-)
65107922 .. 65113455
5534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00273280.1

Sequence Viewer

Length: 2565 bp
ATGACCCTCTTCATTTCCATCCCTTCCCCTCTCTCCGCCCCAATCCTCAAGCTAAACCCTACACCCCACCACCTCTCCTCCTTCCGATTCCTCTCCGGCCACCGCAAGTTCCTCCACGGCCAGCGGTTATCGGTCTCCGTCAGGGCGAAGCCGAAGGACCTCATCCTCGGGAACCCCTCCGTCACCGTCGAGAAGGGCAAGTACAGCTACGATGTCGAAACCCTAATCAACAAGCTCAGCAGCCTCCCGCCACGTGGCAGCATCGCGCGGTGCCTGGACATCTTCAAGAACAAGCTCTCCCTGAACGACTTCGCCCTGGTGTTCAAGGAGTTCGCGGCGCGTGGCGATTGGCAGAGGTCACTGCGGCTGTTCAAGTATATGCAGCGGCAGATATGGTGCAAGCCGAGCGAGCACATCTACACCATCATGATCAGTCTGCTCGGCCGCGAAGGTTTGCTTGACAAATGCGCTGAGATTTTCGACGAAATGCCTAGCCAAGGTGTGATTAGGACTGTGTTTAGCTACACTGCTTTGATTAATGCCTATGGCCGGAATGGATTGTTTGCGGAAATGAGGCATGAGGGGGTTCAACCGGACCTTGTTACTTATAATACATTGCTTAGTGCTTGCGCCGGTAGAGGTTTAGGTGATGAGGCGGAGATGGTGTTTAGGACTATGAATGAGGGTGGGATAGTTCCGGATATAACCACGTATAGTTATCTTGTTGAGACTTTTGGTAAATTGGACAAGCTTGAGAAGTTGGGGTCGATTAAGGAGGCAATGGGTGTGTTTAGGCAGATGCAAGCGGCAGGGTGTATGCCAAATGCGGCTACTTATAGTATTTTGTTGAATTTGTATGGGAGGCATGGGAGGTATGATGATGTTCGGGAGCTTTTTCTTGAGATGAAAGTGAGCAATACGGAGCCAGATGCTGCTACGTATAATATTCTCATACAGGTGTTTGGTGAGGGTGGGTATTTTCGGGAGGTGGTGACTTTGTTTCATGATATGGTAGAGGAGAATATTGAGCCGAATATGGAGACGTATGAAGGATTGATATATGCTTGTGGAAAAGGAGGGCTTCATGAGGATGCCAAGAACATATTGCTTCACATGAATGAGAAGGGAATAGTGCCTAGTTCCAAGGCTTATACAGGAGTTATTGAAGCATATGGGCAAGCAGCACTGTATGACGAAGCTCTTGTTGCCTTCAACACAATGCATGAAGTGGGCAGCAGACCGTCAGTTGAAAGCTACAACTCGCTTATTCATGCATATGCAAGAGGTGGACTATACAAGGAGACTGAACAAATCTTGTCGATAATGGTTGAGGTTGGCATTGCAAGGAATGCCAGTTCCTTCAATGGAATGATTGAAGCTTTTAGGCAGGGAGGTCAGTTTGAAGAAGCTATAAAGACGTACGTTGAGATGGAAAAGAGAAGATGTGATCCTGATGAGTGCACTCTTGAAGCAGTCTTAAGTGTTTATAGTGTTGCAGGTCTTGTTAATGAGTGTGAGGAGCACTTCGAAGAGATTAAAGCTTCAGGGATATTGCCCAGCGTCATGTGCTACTGCATGATGCTTGCTGTTTATGCAAAGACTGACAGGTGGGATGATGCCAACAAGTTGCTGAATGAGATGCTCACAAATAGGGTATCAAATATTCATCAAGTGATGGGACAGATGATAAAGGGAGATTATGATGACGAATCTAACTGGCAGATGGTAGAGTATGTTTTTGACAAACTAAAATCTGAAGGATGTGGGCTTGGAATGAGGTTCTACAACACTTTGATAGAAGCACTTTGGTGGCTGGGTCAGAAACAAAGAGCTGTGCGAGTTCTTAGTGAAGCAACTCAACGGGGGCTGTTTCCTGAACTTCTCCGTAAAAATAAACTTGTTTGGTCTATAGATGTGCACAGGATGTGGGAAGGTGGTGCATATGCAGCAATGTCACTTTGGCTAAACAATATGTATGAGATGTTCCTGAATGGCGAGGATCTTCCCTATGTTGCAACTGTTGTTGTAGTACGGGGGAAGATGGAAAAAAATTCAACAACACAAGATCTGCCGGTTGCAAAGGCCGCCTATTCATTCCTGCAGGATAACATGTCGGGAGCATTCAGTTTCCCAAAATGGAACAATGGTCGGATTCTCTGCCAAAGGTCTCAGCTCAAGAAGATTCTGTCAAGCATTGAACCATTTACAGACGGCTCCAGCAGTAAAAGTATATGTACTTTAAGTAACTCCCCTTTTCCTCCTCCAGGCATAAAAACATCACCCACCGATGTTGATAGTGTTCTGTTTAATGGTACCACTTCTGATGCAACAAGTAGGACAAGAACAGAGCTTTTGACAAGCACACCAGCTGAAGTTTTAGCACGGCAAGATGGCAGCATTGACCAAACTGCAAATACTGTGCGCAAGCCACGGTCTTCCAAGTGCCCAGTACAGCAGTACTTAAAGCATTGGACAGCCAATGGTACCAAACATGGAATCAAACGTACTTCTGTCGCCAAGTACCAAGTAACATTGAATCAGCCTCAACTACCGCTAATCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

854

Amino Acids

95.93

Weight (kDa)

6.66

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 135 - 183 2.4e-08 PPR repeat family
PPR PF01535 139 - 168 4.8e-06 PPR repeat
PPR_2 PF13041 187 - 211 1e-05 PPR repeat family
PPR_3 PF13812 187 - 247 6.9e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 195 - 226 7.1e-06 PPR repeat
PPR_2 PF13041 198 - 245 2.7e-11 PPR repeat family
PPR_2 PF13041 252 - 284 5.4e-07 PPR repeat family
PPR_3 PF13812 263 - 323 5.3e-08 Pentatricopeptide repeat domain
PPR PF01535 278 - 305 6e-06 PPR repeat
PPR_2 PF13041 281 - 321 2.4e-09 PPR repeat family
PPR_2 PF13041 313 - 358 3.2e-09 PPR repeat family
PPR_3 PF13812 333 - 393 6.3e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 379 - 428 3.7e-06 PPR repeat family
PPR_3 PF13812 406 - 461 4.5e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 414 - 461 3e-06 PPR repeat family
PPR_2 PF13041 450 - 493 2.5e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 609
Acc16I TGCGCA 1 cut(s) 2423
Acc36I ACCTGC 1 cut(s) 1487
Acc65I GGTACC 2 cut(s) 2312, 2483
AccB1I GGYRCC 3 cut(s) 270, 2312, 2483
AccII CGCG 5 cut(s) 266, 268, 335, 340, 447
AccIII TCCGGA 1 cut(s) 697
AclWI GGATC 2 cut(s) 1442, 2007
AcoI YGGCCR 4 cut(s) 97, 118, 442, 547
AcsI RAATTY 2 cut(s) 850, 2050
AcuI CTGAAG 3 cut(s) 1527, 1776, 2391
AcvI CACGTG 1 cut(s) 254
AfiI CCNNNNNNNGG 4 cut(s) 254, 497, 549, 2264
AflII CTTAAG 1 cut(s) 1477
AflIII ACRYGT 1 cut(s) 2109
AjnI CCWGG 3 cut(s) 273, 315, 2263
AleI CACNNNNGTG 1 cut(s) 1807
AloI GAACNNNNNNTCC 4 cut(s) 281, 313, 1339, 1371
Alw21I GWGCWC 4 cut(s) 414, 1463, 1524, 1920
Alw26I GTCTC 5 cut(s) 139, 722, 1034, 1295, 2172
Alw44I GTGCAC 2 cut(s) 1459, 1916
AlwI GGATC 2 cut(s) 1442, 2007
AlwNI CAGNNNCTG 1 cut(s) 932
Ama87I CYCGRG 1 cut(s) 167
Aor13HI TCCGGA 1 cut(s) 697
AoxI GGCC 5 cut(s) 97, 118, 442, 547, 2082
ApaLI GTGCAC 2 cut(s) 1459, 1916
ApeKI GCWGC 8 cut(s) 240, 258, 382, 932, 1181, 1233, 1946, 2394
ApoI RAATTY 2 cut(s) 850, 2050
AseI ATTAAT 1 cut(s) 537
Asp700I GAANNNNTTC 1 cut(s) 308
Asp718I GGTACC 2 cut(s) 2312, 2483
AspLEI GCGC 5 cut(s) 268, 340, 470, 632, 2424
AspS9I GGNCC 2 cut(s) 157, 595
AsuHPI GGTGA 5 cut(s) 175, 659, 977, 1003, 2271
AsuII TTCGAA 1 cut(s) 1527
AvaI CYCGRG 1 cut(s) 167
AvaII GGWCC 2 cut(s) 157, 595
BaeGI GKGCMC 3 cut(s) 1463, 1920, 2447
BanI GGYRCC 3 cut(s) 270, 2312, 2483
BarI GAAGNNNNNNTAC 2 cut(s) 185, 217
BbrPI CACGTG 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 2427
Bbv12I GWGCWC 4 cut(s) 414, 1463, 1524, 1920
BbvI GCAGC 8 cut(s) 252, 270, 394, 919, 1193, 1245, 1958, 2406
BccI CCATC 8 cut(s) 26, 431, 655, 1423, 1669, 1717, 2035, 2384
BceAI ACGGC 3 cut(s) 133, 2227, 2399
BciT130I CCWGG 3 cut(s) 275, 317, 2265
BclI TGATCA 1 cut(s) 429
BcoDI GTCTC 5 cut(s) 139, 722, 1034, 1295, 2172
BfaI CTAG 2 cut(s) 492, 1137
BfmI CTRYAG 2 cut(s) 1908, 2099
BfrI CTTAAG 1 cut(s) 1477
BfuAI ACCTGC 1 cut(s) 1487
BglII AGATCT 1 cut(s) 2065
BlpI GCTNAGC 1 cut(s) 236
BmcAI AGTACT 1 cut(s) 2459
Bme1390I CCNGG 3 cut(s) 275, 317, 2265
Bme18I GGWCC 2 cut(s) 157, 595
BmeT110I CYCGRG 1 cut(s) 167
BmgT120I GGNCC 2 cut(s) 157, 595
BmiI GGNNCC 6 cut(s) 173, 272, 924, 2215, 2314, 2485
BmrFI CCNGG 3 cut(s) 275, 317, 2265
BmrI ACTGGG 1 cut(s) 2441
BmsI GCATC 8 cut(s) 270, 789, 919, 1081, 1569, 1606, 1629, 2314
BmuI ACTGGG 1 cut(s) 2441
BpiI GAAGAC 1 cut(s) 2427
BpmI CTGGAG 2 cut(s) 2200, 2247
Bpu1102I GCTNAGC 1 cut(s) 236
Bpu14I TTCGAA 1 cut(s) 1527
BpuEI CTTGAG 4 cut(s) 32, 773, 920, 2159
BsaAI YACGTR 3 cut(s) 254, 711, 939
BsaI GGTCTC 2 cut(s) 139, 2172
BsaJI CCNNGG 6 cut(s) 115, 166, 315, 496, 1143, 2429
BsaWI WCCGGW 2 cut(s) 592, 697
BsaXI ACNNNNNCTCC 8 cut(s) 59, 62, 89, 92, 281, 311, 650, 680
Bsc4I CCNNNNNNNGG 4 cut(s) 254, 497, 549, 2264
Bse118I RCCGGY 2 cut(s) 632, 2071
Bse1I ACTGG 3 cut(s) 1353, 1721, 2447
Bse3DI GCAATG 4 cut(s) 614, 786, 1338, 1956
BseAI TCCGGA 1 cut(s) 697
BseBI CCWGG 3 cut(s) 275, 317, 2265
BseDI CCNNGG 6 cut(s) 115, 166, 315, 496, 1143, 2429
BseGI GGATG 6 cut(s) 18, 162, 1096, 1618, 1766, 1929
BseLI CCNNNNNNNGG 4 cut(s) 254, 497, 549, 2264
BseMI GCAATG 4 cut(s) 614, 786, 1338, 1956
BseMII CTCAG 3 cut(s) 250, 462, 2183
BseNI ACTGG 3 cut(s) 1353, 1721, 2447
BseRI GAGGAG 4 cut(s) 67, 1031, 1532, 2250
BseSI GKGCMC 3 cut(s) 1463, 1920, 2447
BseX3I CGGCCG 1 cut(s) 442
BseXI GCAGC 8 cut(s) 252, 270, 394, 919, 1193, 1245, 1958, 2406
BseYI CCCAGC 2 cut(s) 1556, 1813
Bsh1236I CGCG 5 cut(s) 266, 268, 335, 340, 447
Bsh1285I CGRYCG 1 cut(s) 445
BshFI GGCC 5 cut(s) 99, 120, 444, 549, 2084
BshNI GGYRCC 3 cut(s) 270, 2312, 2483
BsiEI CGRYCG 1 cut(s) 445
BsiHKAI GWGCWC 4 cut(s) 414, 1463, 1524, 1920
BsiHKCI CYCGRG 1 cut(s) 167
BsiSI CCGG 6 cut(s) 96, 550, 593, 633, 698, 2072
BsiWI CGTACG 1 cut(s) 1419
BslFI GGGAC 1 cut(s) 1692
BslI CCNNNNNNNGG 4 cut(s) 254, 497, 549, 2264
BsmAI GTCTC 5 cut(s) 139, 722, 1034, 1295, 2172
BsmBI CGTCTC 1 cut(s) 1034
BsmFI GGGAC 1 cut(s) 1692
BsmI GAATGC 2 cut(s) 1354, 2120
BsnI GGCC 5 cut(s) 99, 120, 444, 549, 2084
Bso31I GGTCTC 2 cut(s) 139, 2172
BsoBI CYCGRG 1 cut(s) 167
Bsp119I TTCGAA 1 cut(s) 1527
Bsp1286I GDGCHC 5 cut(s) 414, 1463, 1524, 1920, 2447
Bsp13I TCCGGA 1 cut(s) 697
Bsp143I GATC 4 cut(s) 429, 1447, 1999, 2065
Bsp1720I GCTNAGC 1 cut(s) 236
BspANI GGCC 5 cut(s) 99, 120, 444, 549, 2084
BspCNI CTCAG 3 cut(s) 249, 463, 2182
BspEI TCCGGA 1 cut(s) 697
BspFNI CGCG 5 cut(s) 266, 268, 335, 340, 447
BspHI TCATGA 3 cut(s) 426, 1003, 1084
BspLI GGNNCC 6 cut(s) 173, 272, 924, 2215, 2314, 2485
BspMAI CTGCAG 1 cut(s) 2103
BspMI ACCTGC 1 cut(s) 1487
BspPI GGATC 2 cut(s) 1442, 2007
BspT104I TTCGAA 1 cut(s) 1527
BspT107I GGYRCC 3 cut(s) 270, 2312, 2483
BspTI CTTAAG 1 cut(s) 1477
BspTNI GGTCTC 2 cut(s) 139, 2172
BsrDI GCAATG 4 cut(s) 614, 786, 1338, 1956
BsrFI RCCGGY 2 cut(s) 632, 2071
BsrI ACTGG 3 cut(s) 1353, 1721, 2447
BssAI RCCGGY 2 cut(s) 632, 2071
BssECI CCNNGG 6 cut(s) 115, 166, 315, 496, 1143, 2429
BssMI GATC 4 cut(s) 429, 1447, 1999, 2065
BssT1I CCWWGG 2 cut(s) 496, 1143
Bst2UI CCWGG 3 cut(s) 275, 317, 2265
Bst4CI ACNGT 7 cut(s) 187, 514, 1188, 1242, 2020, 2419, 2433
Bst6I CTCTTC 2 cut(s) 14, 1524
BstAFI CTTAAG 1 cut(s) 1477
BstAPI GCANNNNNTGC 1 cut(s) 1349
BstBAI YACGTR 3 cut(s) 254, 711, 939
BstBI TTCGAA 1 cut(s) 1527
BstC8I GCNNGC 8 cut(s) 122, 401, 410, 628, 804, 1179, 1584, 2426
BstDEI CTNAG 5 cut(s) 236, 471, 620, 1844, 2169
BstDSI CCRYGG 2 cut(s) 115, 2429
BstENI CCTNNNNNAGG 2 cut(s) 495, 2262
BstF5I GGATG 6 cut(s) 18, 162, 1096, 1618, 1766, 1929
BstFNI CGCG 5 cut(s) 266, 268, 335, 340, 447
BstHHI GCGC 5 cut(s) 268, 340, 470, 632, 2424
BstKTI GATC 4 cut(s) 432, 1450, 2002, 2068
BstMAI GTCTC 5 cut(s) 139, 722, 1034, 1295, 2172
BstMBI GATC 4 cut(s) 429, 1447, 1999, 2065
BstMCI CGRYCG 1 cut(s) 445
BstMWI GCNNNNNNNGC 9 cut(s) 204, 405, 409, 1205, 1349, 1566, 1592, 1946, 2084
BstNI CCWGG 3 cut(s) 275, 317, 2265
BstNSI RCATGY 1 cut(s) 2113
BstSCI CCNGG 3 cut(s) 273, 315, 2263
BstSFI CTRYAG 2 cut(s) 1908, 2099
BstSLI GKGCMC 3 cut(s) 1463, 1920, 2447
BstSNI TACGTA 1 cut(s) 939
BstUI CGCG 5 cut(s) 266, 268, 335, 340, 447
BstV1I GCAGC 8 cut(s) 252, 270, 394, 919, 1193, 1245, 1958, 2406
BstV2I GAAGAC 1 cut(s) 2427
BstX2I RGATCY 2 cut(s) 1999, 2065
BstYI RGATCY 2 cut(s) 1999, 2065
BstZI CGGCCG 1 cut(s) 442
BsuRI GGCC 5 cut(s) 99, 120, 444, 549, 2084
BtgI CCRYGG 2 cut(s) 115, 2429
BtgZI GCGATG 1 cut(s) 247
BtsCI GGATG 6 cut(s) 18, 162, 1096, 1618, 1766, 1929
BtsI GCAGTG 2 cut(s) 359, 525
BtsIMutI CAGTG 3 cut(s) 359, 525, 1184
BveI ACCTGC 1 cut(s) 1487
Cac8I GCNNGC 8 cut(s) 122, 401, 410, 628, 804, 1179, 1584, 2426
CaiI CAGNNNCTG 1 cut(s) 932
CciI TCATGA 3 cut(s) 426, 1003, 1084
CfoI GCGC 5 cut(s) 268, 340, 470, 632, 2424
Cfr10I RCCGGY 2 cut(s) 632, 2071
Cfr13I GGNCC 2 cut(s) 157, 595
CseI GACGC 1 cut(s) 1549
CspCI CAANNNNNGTGG 2 cut(s) 2272, 2307
DdeI CTNAG 5 cut(s) 236, 471, 620, 1844, 2169
DpnI GATC 4 cut(s) 431, 1449, 2001, 2067
DpnII GATC 4 cut(s) 429, 1447, 1999, 2065
EaeI YGGCCR 4 cut(s) 97, 118, 442, 547
EagI CGGCCG 1 cut(s) 442
Eam1104I CTCTTC 2 cut(s) 14, 1524
EarI CTCTTC 2 cut(s) 14, 1524
EciI GGCGGA 2 cut(s) 25, 671
EclXI CGGCCG 1 cut(s) 442
Eco105I TACGTA 1 cut(s) 939
Eco130I CCWWGG 2 cut(s) 496, 1143
Eco31I GGTCTC 2 cut(s) 139, 2172
Eco47I GGWCC 2 cut(s) 157, 595
Eco52I CGGCCG 1 cut(s) 442
Eco57I CTGAAG 3 cut(s) 1527, 1776, 2391
Eco72I CACGTG 1 cut(s) 254
Eco88I CYCGRG 1 cut(s) 167
EcoNI CCTNNNNNAGG 2 cut(s) 495, 2262
EcoO109I RGGNCCY 1 cut(s) 157
EcoRII CCWGG 3 cut(s) 273, 315, 2263
EcoT14I CCWWGG 2 cut(s) 496, 1143
EcoT22I ATGCAT 2 cut(s) 1224, 1276
ErhI CCWWGG 2 cut(s) 496, 1143
Esp3I CGTCTC 1 cut(s) 1034
FalI AAGNNNNNCTT 4 cut(s) 441, 473, 1065, 1097
FaqI GGGAC 1 cut(s) 1692
FauI CCCGC 1 cut(s) 255
FauNDI CATATG 3 cut(s) 1171, 1276, 1942
FbaI TGATCA 1 cut(s) 429
FokI GGATG 6 cut(s) 5, 149, 1103, 1625, 1773, 1936
FspBI CTAG 2 cut(s) 492, 1137
FspI TGCGCA 1 cut(s) 2423
GlaI GCGC 5 cut(s) 267, 339, 469, 631, 2423
GsaI CCCAGC 2 cut(s) 1560, 1817
GsuI CTGGAG 2 cut(s) 2200, 2247
HaeIII GGCC 5 cut(s) 99, 120, 444, 549, 2084
HapII CCGG 6 cut(s) 96, 550, 593, 633, 698, 2072
HgaI GACGC 1 cut(s) 1549
HhaI GCGC 5 cut(s) 268, 340, 470, 632, 2424
Hin6I GCGC 5 cut(s) 266, 338, 468, 630, 2422
HinP1I GCGC 5 cut(s) 266, 338, 468, 630, 2422
HindIII AAGCTT 3 cut(s) 749, 1377, 1539
HinfI GANTC 6 cut(s) 87, 1709, 2152, 2182, 2496, 2536
HpaII CCGG 6 cut(s) 96, 550, 593, 633, 698, 2072
HphI GGTGA 5 cut(s) 175, 659, 977, 1003, 2271
Hpy166II GTNNAC 3 cut(s) 1289, 1461, 1918
Hpy188I TCNGA 5 cut(s) 86, 1756, 1821, 2151, 2323
Hpy8I GTNNAC 3 cut(s) 1289, 1461, 1918
Hpy99I CGWCG 2 cut(s) 191, 485
HpyCH4III ACNGT 7 cut(s) 187, 514, 1188, 1242, 2020, 2419, 2433
HpyCH4IV ACGT 7 cut(s) 253, 710, 938, 1043, 1418, 1422, 2503
HpyF10VI GCNNNNNNNGC 9 cut(s) 204, 405, 409, 1205, 1349, 1566, 1592, 1946, 2084
HpyF3I CTNAG 5 cut(s) 236, 471, 620, 1844, 2169
HpySE526I ACGT 7 cut(s) 253, 710, 938, 1043, 1418, 1422, 2503
HspAI GCGC 5 cut(s) 266, 338, 468, 630, 2422
Kpn2I TCCGGA 1 cut(s) 697
KpnI GGTACC 2 cut(s) 2316, 2487
Ksp22I TGATCA 1 cut(s) 429
Kzo9I GATC 4 cut(s) 429, 1447, 1999, 2065
LmnI GCTCC 5 cut(s) 889, 922, 1519, 2117, 2219
Lsp1109I GCAGC 8 cut(s) 252, 270, 394, 919, 1193, 1245, 1958, 2406
LweI GCATC 8 cut(s) 270, 789, 919, 1081, 1569, 1606, 1629, 2314
MaeI CTAG 2 cut(s) 492, 1137
MaeII ACGT 7 cut(s) 253, 710, 938, 1043, 1418, 1422, 2503
MaeIII GTNAC 7 cut(s) 181, 357, 601, 991, 1953, 2243, 2527
MalI GATC 4 cut(s) 431, 1449, 2001, 2067
MboI GATC 4 cut(s) 429, 1447, 1999, 2065
MboII GAAGA 8 cut(s) 274, 1415, 1452, 1541, 1994, 2050, 2191, 2427
MflI RGATCY 2 cut(s) 1999, 2065
MhlI GDGCHC 5 cut(s) 414, 1463, 1524, 1920, 2447
MluCI AATT 3 cut(s) 740, 850, 2050
MmeI TCCRAC 1 cut(s) 2129
Mph1103I ATGCAT 2 cut(s) 1224, 1276
MroI TCCGGA 1 cut(s) 697
MroXI GAANNNNTTC 1 cut(s) 308
MseI TTAA 8 cut(s) 537, 771, 1478, 1506, 1536, 2240, 2307, 2462
MslI CAYNNNNRTG 6 cut(s) 425, 956, 1089, 1116, 1275, 1807
MspA1I CMGCKG 3 cut(s) 124, 385, 2369
MspCI CTTAAG 1 cut(s) 1477
MspI CCGG 6 cut(s) 96, 550, 593, 633, 698, 2072
MspR9I CCNGG 3 cut(s) 275, 317, 2265
Mva1269I GAATGC 2 cut(s) 1354, 2120
MvaI CCWGG 3 cut(s) 275, 317, 2265
MvnI CGCG 5 cut(s) 266, 268, 335, 340, 447
MwoI GCNNNNNNNGC 9 cut(s) 204, 405, 409, 1205, 1349, 1566, 1592, 1946, 2084
NdeI CATATG 3 cut(s) 1171, 1276, 1942
NdeII GATC 4 cut(s) 429, 1447, 1999, 2065
NlaIV GGNNCC 6 cut(s) 173, 272, 924, 2215, 2314, 2485
NmeAIII GCCGAG 2 cut(s) 420, 429
NmuCI GTSAC 4 cut(s) 181, 357, 991, 1953
NsbI TGCGCA 1 cut(s) 2423
NsiI ATGCAT 2 cut(s) 1224, 1276
NspI RCATGY 1 cut(s) 2113
NspV TTCGAA 1 cut(s) 1527
OliI CACNNNNGTG 1 cut(s) 1807
PagI TCATGA 3 cut(s) 426, 1003, 1084
PciI ACATGT 1 cut(s) 2109
PctI GAATGC 2 cut(s) 1354, 2120
PdmI GAANNNNTTC 1 cut(s) 308
PfeI GAWTC 6 cut(s) 87, 1709, 2152, 2182, 2496, 2536
Pfl23II CGTACG 1 cut(s) 1419
PmaCI CACGTG 1 cut(s) 254
PmlI CACGTG 1 cut(s) 254
Ppu21I YACGTR 3 cut(s) 254, 711, 939
PpuMI RGGWCCY 1 cut(s) 157
PscI ACATGT 1 cut(s) 2109
PshBI ATTAAT 1 cut(s) 537
PsiI TTATAA 1 cut(s) 609
Psp5II RGGWCCY 1 cut(s) 157
Psp6I CCWGG 3 cut(s) 273, 315, 2263
PspCI CACGTG 1 cut(s) 254
PspFI CCCAGC 2 cut(s) 1556, 1813
PspGI CCWGG 3 cut(s) 273, 315, 2263
PspLI CGTACG 1 cut(s) 1419
PspN4I GGNNCC 6 cut(s) 173, 272, 924, 2215, 2314, 2485
PspPI GGNCC 2 cut(s) 157, 595
PspPPI RGGWCCY 1 cut(s) 157
PsrI GAACNNNNNNTAC 2 cut(s) 1967, 1999
PstI CTGCAG 1 cut(s) 2103
PstNI CAGNNNCTG 1 cut(s) 932
PsuI RGATCY 2 cut(s) 1999, 2065
PvuII CAGCTG 1 cut(s) 2369
RseI CAYNNNNRTG 6 cut(s) 425, 956, 1089, 1116, 1275, 1807
SaqAI TTAA 8 cut(s) 537, 771, 1478, 1506, 1536, 2240, 2307, 2462
Sau3AI GATC 4 cut(s) 429, 1447, 1999, 2065
Sau96I GGNCC 2 cut(s) 157, 595
SbfI CCTGCAGG 1 cut(s) 2103
ScaI AGTACT 1 cut(s) 2459
ScrFI CCNGG 3 cut(s) 275, 317, 2265
SdaI CCTGCAGG 1 cut(s) 2103
SduI GDGCHC 5 cut(s) 414, 1463, 1524, 1920, 2447
SfaNI GCATC 8 cut(s) 270, 789, 919, 1081, 1569, 1606, 1629, 2314
SfcI CTRYAG 2 cut(s) 1908, 2099
SfuI TTCGAA 1 cut(s) 1527
SinI GGWCC 2 cut(s) 157, 595
SmiMI CAYNNNNRTG 6 cut(s) 425, 956, 1089, 1116, 1275, 1807
SmlI CTYRAG 5 cut(s) 47, 752, 899, 1477, 2174
SmoI CTYRAG 5 cut(s) 47, 752, 899, 1477, 2174
SnaBI TACGTA 1 cut(s) 939
Sse8387I CCTGCAGG 1 cut(s) 2103
Sse9I AATT 3 cut(s) 740, 850, 2050
SspI AATATT 3 cut(s) 946, 1024, 1663
SspMI CTAG 2 cut(s) 492, 1137
StyD4I CCNGG 3 cut(s) 273, 315, 2263
StyI CCWWGG 2 cut(s) 496, 1143
TaaI ACNGT 7 cut(s) 187, 514, 1188, 1242, 2020, 2419, 2433
TaiI ACGT 7 cut(s) 256, 713, 941, 1046, 1421, 1425, 2506
TaqI TCGA 6 cut(s) 189, 216, 480, 767, 1319, 1527
TaqII GACCGA 1 cut(s) 121
TasI AATT 3 cut(s) 740, 850, 2050
TatI WGTACW 4 cut(s) 201, 2234, 2449, 2457
TauI GCSGC 7 cut(s) 338, 367, 388, 447, 809, 830, 2087
TfiI GAWTC 6 cut(s) 87, 1709, 2152, 2182, 2496, 2536
Tru1I TTAA 8 cut(s) 537, 771, 1478, 1506, 1536, 2240, 2307, 2462
Tru9I TTAA 8 cut(s) 537, 771, 1478, 1506, 1536, 2240, 2307, 2462
TscAI CASTG 3 cut(s) 366, 532, 1191
TseFI GTSAC 4 cut(s) 181, 357, 991, 1953
TseI GCWGC 8 cut(s) 240, 258, 382, 932, 1181, 1233, 1946, 2394
Tsp45I GTSAC 4 cut(s) 181, 357, 991, 1953
TspGWI ACGGA 4 cut(s) 127, 169, 935, 1874
TspRI CASTG 3 cut(s) 366, 532, 1191
Vha464I CTTAAG 1 cut(s) 1477
VneI GTGCAC 2 cut(s) 1459, 1916
VpaK11BI GGWCC 2 cut(s) 157, 595
VspI ATTAAT 1 cut(s) 537
XagI CCTNNNNNAGG 2 cut(s) 495, 2262
XapI RAATTY 2 cut(s) 850, 2050
XceI RCATGY 1 cut(s) 2113
XmnI GAANNNNTTC 1 cut(s) 308
XspI CTAG 2 cut(s) 492, 1137
ZrmI AGTACT 1 cut(s) 2459
Zsp2I ATGCAT 2 cut(s) 1224, 1276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.