MD06G1183200.v1.1

Pentatricopeptide repeat-containing protein At1g74850

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0009497
Physical Location & Seq
Forward (+)
32160322 .. 32164252
3931 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1183200.v1.1.491

Sequence Viewer

Length: 2631 bp
ATGTCCTTCTCCCCCACGCTCTCCATTTCCAGCTCTTCGCCTATATCCGCCTCAGTTTACAAAATTAACCACAAGTCCCACCAGCTCACTGTCGTCACCAAAGCCCCGGACTCGTCCGTGCACCGGAGATTCTTATCCGTCCGCCATAACTTCCTCTCCGGCGACCGTCGGCTGGCGTTCTCTGCCAGGGCGAAGCCGAAGGATCTCATCCTCGGGAACCCGTCGGTGACGCTGGAGAAGGGGAAGTACAGCTACGACGTCGAGACCCTAATTAACAAACTCAGTAGCCTCCCTCCGCGCGGAAGCATCGCTCGGTGCCTCGACATTTTCAAGAACAAGCTGTCGCTGAACGATTTCGCTTTGGTGTTCAAGGAGTTCGCCGCGCGCGGTGATTGGCAGCGGTCGCTCCGCCTCTTCAAGTATATGCAGCGCCAGATATGGTGCAAGCCGAACGAGCACATATACACGATAATGTTCAGCTTGCTCGGCCGCGAGGGGCTGCTGGATAAGTGCACCGAGGTGTTCGACGAAATGCCTAGCCAGGGCGTGGTCCGCAGCGTCTTCAGCTACACCGCATTGATCAATGCCTACGGGCGTAACGGTCAGTACGAAACCTCCCTTGAATTTCTTGATAGAATGAAGAAGGATAAGGTTTCGCCGAGTATTTTGACTTATAATACTGTGCTCAATGCTTGTGCAAGAGGTGGGTTGGATTGGGAAGGGTTGTTAGGATTGTTTGCCGAAATGCGGCACGATGGAATACTGCCGGACCTTGTTACTTATAATACTTTGCTTAGTGCTTGTGCTGGTAGAGGATTAGGTGATGAGGCGGAGATGGTGTTCGGGACTATGAATGAGGGTGGGATTGTTCCGGATATAACCACTTATCGTTACCTTGTTGAAACTTTTGGTAAATTGGACAAGCTTGAGAGAGTCTCGGAGCTTCTTAAAGAGATGGAAGCTGGAGGGAATTTGCCTGATATTACGTCGTATAATGTGTTACTAGAGGCATATGCACAATTGGGGTCGATTAGAGAGTCAATGGGTGTGTTTAGGCAGATGCAGGCAGCAGGGTGTATGCCAAATGCCGCCACTTACAGTATTTTGTTGAATCTGTACGGGAGTCATGGAAGGTATGATGATGTTCGGGAGCTTTTTCTTGAGATGAAAGTGAGCAATACAGTGCCAGATGCAGCTACGTATAACATTCTCATACAAGTGTTTGGGGAGGGTGGGTATTTTAAGGAGGTAGTGACTTTGTTTCATGATATGGTGGAGGAGAAAATTGAGCCCAATATGGAGACGTGCGAAGGGTTGATATATGCTTGTGGAAAGGGAGGACTTCATGAGGATTCCAAGAAAATTTTACTTCACATGCACGAGAATGGAATAGTGCCTAGTACCAAGGCCTATACAGGGGTTATTGAAGCATATGGGCAAGCGGCATTGTATGACGAAGCTCTTGTTGCCTTCAACATAATGAATGAAGTGGGAAGCAAACCATCAGTTGAAAGCTACAACTCGCTGATACATGCATTTGCAAGAGGCGGATTATACAGGGAAACTGAAGCAGTCTTGTTGATAACAGGCGAGGCTGGTGTAGCCAGGAATGTCCATACAGTCAATGGCATGATAGAAGCTTTTAGGCAAGGAGGTCAATTTGAAGAAGCTATCAAGTCATACGTTGAGATGGAAAAGACAAGATGCGAACCTGATGAGTGGACCCTTGAGGCAGTTTTAAGCGTTTACTGCGTTGCAGGTCTTGTTAATGAGTGTGAGGAGCACTTCCAAGAGATTAAAGCCTCAGGAATATTACCCAGTGTCATGTGCTACTGCATGATGCTTGCTGTTTATGCGAGGAGTGACAGGTGGGATGATGCCTATGAGTTGCTGAATGAGATGCTTACCAACAGGCAATCAAATATTCATCAAGTGATTGGGCAGATGATCAAGGGAGACTACGATGATGACTCTAACTGGCAGATGGTAGAATATGTTTTTGACAAAATAAAATCTGAGGGATGCGGGTTGGGAATGAGGTTCTACAACACTCTACTGGAAGCACTTTGGTGGCTGGGTCAGAAACAAAGAGCTGTGCGAGTTCTTAATGAAGCAACACAGCGGGGGCTTTTTCCTGAACTTTTTCGTAAAAATAAACTTGTGGCGTCTGTTGATGTGCACAGGATGTGGCAAGGTGGTGCATATACAGCAATGTCAGTTTGGCTAAACAATATGTATGAGATGTTCCTCAATGGCGAGGATCTACCCCATCTTGCAACTGTCGTCGTAGTACGAGGGAAGATGGAGAAAAGCTCCATATCACAAGAACTACCGATTGCAAAGGCTGCTTATACATTTCTGCAGGATAATATGCCAGCGTCCTTTTCTTTCCCAAAATGGAACAATGGTCGAATACTCTGCCAGCGCCCTCAGCTCAAGCGGATTCTATCAAGCATTGAACCATCCACAGAGGGGTCCGAAGGGAATAATATAACCACGTTAAGTAACTCTCCGTTTCCTCCTCCCGGAACAAACACATCCACCAGTGCTGTCAATAGTGGACGGTATAATGATGCCAGTTCTGATGAAAGAATTAGGACAAGAACAGAGCTTTTGACAAGCACGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

877

Amino Acids

98.22

Weight (kDa)

5.67

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 133 - 236 8.1e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 150 - 195 4.5e-07 PPR repeat family
TPR_24 PF23276 163 - 269 1.7e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 174 - 232 1.2e-14 Pentatricopeptide repeat domain
PPR_2 PF13041 186 - 234 5.9e-13 PPR repeat family
PPR PF01535 188 - 217 5.5e-07 PPR repeat
PPR_long PF17177 201 - 343 1.7e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 245 - 305 1.5e-11 Pentatricopeptide repeat domain
TPR_24 PF23276 247 - 354 5.4e-08 Fungal tetratrico peptide repeats
PPR_2 PF13041 256 - 303 2.8e-10 PPR repeat family
PPR_3 PF13812 315 - 375 3.1e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 326 - 371 2e-11 PPR repeat family
TPR_24 PF23276 330 - 423 4.7e-06 Fungal tetratrico peptide repeats
PPR_2 PF13041 368 - 408 3.4e-08 PPR repeat family
PPR_2 PF13041 396 - 444 9.8e-09 PPR repeat family
PPR_3 PF13812 420 - 480 4.4e-06 Pentatricopeptide repeat domain
TPR_24 PF23276 436 - 563 3.2e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 454 - 513 6.4e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 466 - 515 1.3e-06 PPR repeat family
PPR_2 PF13041 536 - 584 2.7e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 675, 783
AatII GACGTC 1 cut(s) 261
Acc36I ACCTGC 1 cut(s) 1748
AccB1I GGYRCC 1 cut(s) 315
AccB7I CCANNNNNTGG 1 cut(s) 547
AccII CGCG 6 cut(s) 298, 300, 383, 385, 387, 492
AccIII TCCGGA 1 cut(s) 871
AclWI GGATC 2 cut(s) 210, 2268
AcoI YGGCCR 1 cut(s) 487
AcsI RAATTY 3 cut(s) 623, 970, 1362
AcuI CTGAAG 2 cut(s) 547, 1587
AcyI GRCGYC 2 cut(s) 258, 2165
AfaI GTAC 5 cut(s) 248, 608, 1118, 1402, 2292
AfiI CCNNNNNNNGG 9 cut(s) 123, 172, 299, 542, 547, 747, 1416, 2472, 2525
AjiI CACGTC 1 cut(s) 1305
AjnI CCWGG 3 cut(s) 185, 540, 1604
AleI CACNNNNGTG 2 cut(s) 518, 2068
AloI GAACNNNNNNTCC 2 cut(s) 824, 856
Alw21I GWGCWC 6 cut(s) 123, 459, 515, 687, 1785, 2181
Alw26I GTCTC 4 cut(s) 257, 940, 1295, 1950
Alw44I GTGCAC 3 cut(s) 119, 511, 2177
AlwI GGATC 2 cut(s) 210, 2268
Ama87I CYCGRG 1 cut(s) 212
Aor13HI TCCGGA 1 cut(s) 871
AoxI GGCC 2 cut(s) 487, 1407
ApaLI GTGCAC 3 cut(s) 119, 511, 2177
ApeKI GCWGC 7 cut(s) 397, 427, 499, 555, 1067, 1193, 2345
ApoI RAATTY 3 cut(s) 623, 970, 1362
Asp700I GAANNNNTTC 2 cut(s) 353, 2142
AspLEI GCGC 5 cut(s) 300, 385, 387, 432, 2427
AspS9I GGNCC 4 cut(s) 550, 769, 1722, 2475
AsuC2I CCSGG 2 cut(s) 107, 2526
AsuHPI GGTGA 4 cut(s) 88, 238, 401, 833
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 4 cut(s) 550, 769, 1722, 2475
AxyI CCTNAGG 1 cut(s) 1804
BaeGI GKGCMC 3 cut(s) 123, 515, 2181
BanI GGYRCC 1 cut(s) 315
BanII GRGCYC 1 cut(s) 1293
BarI GAAGNNNNNNTAC 4 cut(s) 230, 236, 262, 268
BauI CACGAG 1 cut(s) 1379
BbsI GAAGAC 1 cut(s) 553
Bbv12I GWGCWC 6 cut(s) 123, 459, 515, 687, 1785, 2181
BbvCI CCTCAGC 1 cut(s) 2430
BbvI GCAGC 7 cut(s) 409, 439, 486, 567, 1079, 1205, 2332
BccI CCATC 9 cut(s) 749, 829, 949, 1510, 1684, 1978, 2277, 2296, 2470
BcgI CGANNNNNNTGC 2 cut(s) 2400, 2434
BciT130I CCWGG 3 cut(s) 187, 542, 1606
BclI TGATCA 2 cut(s) 579, 1947
BcnI CCSGG 2 cut(s) 107, 2526
BcoDI GTCTC 4 cut(s) 257, 940, 1295, 1950
BfaI CTAG 3 cut(s) 537, 1004, 1398
BfmI CTRYAG 1 cut(s) 2360
BfoI RGCGCY 2 cut(s) 433, 2428
BfuAI ACCTGC 1 cut(s) 1748
Bme1390I CCNGG 5 cut(s) 107, 187, 542, 1606, 2526
Bme18I GGWCC 4 cut(s) 550, 769, 1722, 2475
BmeT110I CYCGRG 1 cut(s) 212
BmgBI CACGTC 1 cut(s) 1305
BmgT120I GGNCC 4 cut(s) 550, 769, 1722, 2475
BmiI GGNNCC 4 cut(s) 218, 317, 1724, 2476
BmrFI CCNGG 5 cut(s) 107, 187, 542, 1606, 2526
BmrI ACTGGG 1 cut(s) 1812
BmsI GCATC 9 cut(s) 315, 1050, 1180, 1694, 1830, 1867, 1890, 2012, 2563
BmuI ACTGGG 1 cut(s) 1812
BpiI GAAGAC 1 cut(s) 553
BplI GAGNNNNNCTC 4 cut(s) 920, 952, 2297, 2329
BpmI CTGGAG 2 cut(s) 254, 984
Bpu10I CCTNAGC 1 cut(s) 2430
BpuEI CTTGAG 4 cut(s) 947, 1181, 1748, 2420
BpuMI CCSGG 2 cut(s) 107, 2526
BsaAI YACGTR 1 cut(s) 1200
BsaBI GATNNNNATC 1 cut(s) 133
BsaHI GRCGYC 2 cut(s) 258, 2165
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 6 cut(s) 105, 186, 211, 516, 541, 1404
BsaWI WCCGGW 2 cut(s) 123, 871
BsaXI ACNNNNNCTCC 8 cut(s) 140, 170, 599, 629, 824, 854, 1220, 1250
Bsc4I CCNNNNNNNGG 9 cut(s) 123, 172, 299, 542, 547, 747, 1416, 2472, 2525
Bse1I ACTGG 5 cut(s) 1818, 1982, 2061, 2544, 2577
Bse21I CCTNAGG 1 cut(s) 1804
Bse3DI GCAATG 1 cut(s) 2217
Bse8I GATNNNNATC 1 cut(s) 133
BseAI TCCGGA 1 cut(s) 871
BseBI CCWGG 3 cut(s) 187, 542, 1606
BseDI CCNNGG 6 cut(s) 105, 186, 211, 516, 541, 1404
BseGI GGATG 6 cut(s) 207, 1879, 2027, 2190, 2462, 2537
BseJI GATNNNNATC 1 cut(s) 133
BseLI CCNNNNNNNGG 9 cut(s) 123, 172, 299, 542, 547, 747, 1416, 2472, 2525
BseMI GCAATG 1 cut(s) 2217
BseMII CTCAG 5 cut(s) 66, 295, 1818, 2007, 2444
BseNI ACTGG 5 cut(s) 1818, 1982, 2061, 2544, 2577
BsePI GCGCGC 1 cut(s) 383
BseRI GAGGAG 4 cut(s) 1292, 1793, 1873, 2511
BseSI GKGCMC 3 cut(s) 123, 515, 2181
BseX3I CGGCCG 1 cut(s) 487
BseXI GCAGC 7 cut(s) 409, 439, 486, 567, 1079, 1205, 2332
BseYI CCCAGC 1 cut(s) 2074
Bsh1236I CGCG 6 cut(s) 298, 300, 383, 385, 387, 492
Bsh1285I CGRYCG 3 cut(s) 166, 404, 490
BshFI GGCC 2 cut(s) 489, 1409
BshNI GGYRCC 1 cut(s) 315
BsiEI CGRYCG 3 cut(s) 166, 404, 490
BsiHKAI GWGCWC 6 cut(s) 123, 459, 515, 687, 1785, 2181
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 6 cut(s) 107, 124, 159, 767, 872, 2526
BslFI GGGAC 2 cut(s) 61, 859
BslI CCNNNNNNNGG 9 cut(s) 123, 172, 299, 542, 547, 747, 1416, 2472, 2525
BsmAI GTCTC 4 cut(s) 257, 940, 1295, 1950
BsmBI CGTCTC 1 cut(s) 1295
BsmFI GGGAC 2 cut(s) 61, 859
BsnI GGCC 2 cut(s) 489, 1409
Bso31I GGTCTC 1 cut(s) 257
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 7 cut(s) 123, 459, 515, 687, 1293, 1785, 2181
Bsp13I TCCGGA 1 cut(s) 871
Bsp143I GATC 4 cut(s) 202, 579, 1947, 2260
BspANI GGCC 2 cut(s) 489, 1409
BspCNI CTCAG 5 cut(s) 65, 294, 1817, 2008, 2443
BspEI TCCGGA 1 cut(s) 871
BspFNI CGCG 6 cut(s) 298, 300, 383, 385, 387, 492
BspHI TCATGA 2 cut(s) 1264, 1345
BspLI GGNNCC 4 cut(s) 218, 317, 1724, 2476
BspMAI CTGCAG 1 cut(s) 2364
BspMI ACCTGC 1 cut(s) 1748
BspPI GGATC 2 cut(s) 210, 2268
BspQI GCTCTTC 1 cut(s) 40
BspT107I GGYRCC 1 cut(s) 315
BspTNI GGTCTC 1 cut(s) 257
BsrDI GCAATG 1 cut(s) 2217
BsrI ACTGG 5 cut(s) 1818, 1982, 2061, 2544, 2577
BssECI CCNNGG 6 cut(s) 105, 186, 211, 516, 541, 1404
BssHII GCGCGC 1 cut(s) 383
BssMI GATC 4 cut(s) 202, 579, 1947, 2260
BssNI GRCGYC 2 cut(s) 258, 2165
BssSI CACGAG 1 cut(s) 1379
BssT1I CCWWGG 1 cut(s) 1404
Bst2BI CACGAG 1 cut(s) 1379
Bst2UI CCWGG 3 cut(s) 187, 542, 1606
Bst6I CTCTTC 2 cut(s) 40, 419
BstACI GRCGYC 2 cut(s) 258, 2165
BstAPI GCANNNNNTGC 1 cut(s) 2345
BstBAI YACGTR 1 cut(s) 1200
BstC8I GCNNGC 9 cut(s) 174, 385, 446, 482, 1065, 1440, 1845, 2376, 2423
BstDEI CTNAG 6 cut(s) 52, 281, 794, 1804, 2016, 2430
BstF5I GGATG 6 cut(s) 207, 1879, 2027, 2190, 2462, 2537
BstFNI CGCG 6 cut(s) 298, 300, 383, 385, 387, 492
BstH2I RGCGCY 2 cut(s) 433, 2428
BstHHI GCGC 5 cut(s) 300, 385, 387, 432, 2427
BstKTI GATC 4 cut(s) 205, 582, 1950, 2263
BstMAI GTCTC 4 cut(s) 257, 940, 1295, 1950
BstMBI GATC 4 cut(s) 202, 579, 1947, 2260
BstMCI CGRYCG 3 cut(s) 166, 404, 490
BstNI CCWGG 3 cut(s) 187, 542, 1606
BstNSI RCATGY 2 cut(s) 1378, 1535
BstSCI CCNGG 5 cut(s) 105, 185, 540, 1604, 2524
BstSFI CTRYAG 1 cut(s) 2360
BstSLI GKGCMC 3 cut(s) 123, 515, 2181
BstSNI TACGTA 1 cut(s) 1200
BstUI CGCG 6 cut(s) 298, 300, 383, 385, 387, 492
BstV1I GCAGC 7 cut(s) 409, 439, 486, 567, 1079, 1205, 2332
BstV2I GAAGAC 1 cut(s) 553
BstX2I RGATCY 2 cut(s) 202, 2260
BstYI RGATCY 2 cut(s) 202, 2260
BstZI CGGCCG 1 cut(s) 487
Bsu36I CCTNAGG 1 cut(s) 1804
BsuRI GGCC 2 cut(s) 489, 1409
BtgZI GCGATG 1 cut(s) 292
BtrI CACGTC 1 cut(s) 1305
BtsCI GGATG 6 cut(s) 207, 1879, 2027, 2190, 2462, 2537
BtsIMutI CAGTG 4 cut(s) 87, 1188, 1825, 2551
BveI ACCTGC 1 cut(s) 1748
Cac8I GCNNGC 9 cut(s) 174, 385, 446, 482, 1065, 1440, 1845, 2376, 2423
CciI TCATGA 2 cut(s) 1264, 1345
CfoI GCGC 5 cut(s) 300, 385, 387, 432, 2427
Cfr13I GGNCC 4 cut(s) 550, 769, 1722, 2475
CseI GACGC 4 cut(s) 238, 547, 2154, 2367
Csp6I GTAC 5 cut(s) 247, 607, 1117, 1401, 2291
CviAII CATG 8 cut(s) 1127, 1265, 1346, 1375, 1532, 1630, 1825, 1837
CviQI GTAC 5 cut(s) 247, 607, 1117, 1401, 2291
DdeI CTNAG 6 cut(s) 52, 281, 794, 1804, 2016, 2430
DpnI GATC 4 cut(s) 204, 581, 1949, 2262
DpnII GATC 4 cut(s) 202, 579, 1947, 2260
EaeI YGGCCR 1 cut(s) 487
EagI CGGCCG 1 cut(s) 487
Eam1104I CTCTTC 2 cut(s) 40, 419
EarI CTCTTC 2 cut(s) 40, 419
EciI GGCGGA 5 cut(s) 37, 131, 398, 845, 1563
EclXI CGGCCG 1 cut(s) 487
Eco105I TACGTA 1 cut(s) 1200
Eco130I CCWWGG 1 cut(s) 1404
Eco147I AGGCCT 1 cut(s) 1409
Eco24I GRGCYC 1 cut(s) 1293
Eco31I GGTCTC 1 cut(s) 257
Eco47I GGWCC 4 cut(s) 550, 769, 1722, 2475
Eco52I CGGCCG 1 cut(s) 487
Eco57I CTGAAG 2 cut(s) 547, 1587
Eco81I CCTNAGG 1 cut(s) 1804
Eco88I CYCGRG 1 cut(s) 212
EcoRII CCWGG 3 cut(s) 185, 540, 1604
EcoT14I CCWWGG 1 cut(s) 1404
EcoT22I ATGCAT 1 cut(s) 1537
EcoT38I GRGCYC 1 cut(s) 1293
ErhI CCWWGG 1 cut(s) 1404
Esp3I CGTCTC 1 cut(s) 1295
FaeI CATG 8 cut(s) 1130, 1268, 1349, 1378, 1535, 1633, 1828, 1840
FaqI GGGAC 2 cut(s) 61, 859
FatI CATG 8 cut(s) 1126, 1264, 1345, 1374, 1531, 1629, 1824, 1836
FauI CCCGC 2 cut(s) 2018, 2115
FauNDI CATATG 2 cut(s) 1012, 1432
FbaI TGATCA 2 cut(s) 579, 1947
FokI GGATG 6 cut(s) 194, 1886, 2034, 2197, 2449, 2524
FriOI GRGCYC 1 cut(s) 1293
FspBI CTAG 3 cut(s) 537, 1004, 1398
GlaI GCGC 5 cut(s) 299, 384, 386, 431, 2426
GsaI CCCAGC 1 cut(s) 2078
GsuI CTGGAG 2 cut(s) 254, 984
HaeII RGCGCY 2 cut(s) 433, 2428
HaeIII GGCC 2 cut(s) 489, 1409
HapII CCGG 6 cut(s) 107, 124, 159, 767, 872, 2526
HgaI GACGC 4 cut(s) 238, 547, 2154, 2367
HhaI GCGC 5 cut(s) 300, 385, 387, 432, 2427
Hin1I GRCGYC 2 cut(s) 258, 2165
Hin1II CATG 8 cut(s) 1130, 1268, 1349, 1378, 1535, 1633, 1828, 1840
Hin6I GCGC 5 cut(s) 298, 383, 385, 430, 2425
HinP1I GCGC 5 cut(s) 298, 383, 385, 430, 2425
HindIII AAGCTT 2 cut(s) 923, 1638
HinfI GANTC 9 cut(s) 110, 129, 933, 1037, 1111, 1123, 1352, 1970, 2443
HpaII CCGG 6 cut(s) 107, 124, 159, 767, 872, 2526
HphI GGTGA 4 cut(s) 88, 238, 401, 833
Hpy166II GTNNAC 7 cut(s) 58, 121, 513, 1722, 1747, 2179, 2561
Hpy188I TCNGA 5 cut(s) 940, 2017, 2082, 2479, 2584
Hpy8I GTNNAC 7 cut(s) 58, 121, 513, 1722, 1747, 2179, 2561
Hpy99I CGWCG 7 cut(s) 171, 226, 260, 263, 530, 991, 2288
HpyAV CCTTC 9 cut(s) 16, 193, 232, 637, 713, 1125, 1304, 1480, 2474
HpyCH4IV ACGT 6 cut(s) 258, 986, 1199, 1304, 1683, 2498
HpyF3I CTNAG 6 cut(s) 52, 281, 794, 1804, 2016, 2430
HpySE526I ACGT 6 cut(s) 258, 986, 1199, 1304, 1683, 2498
Hsp92I GRCGYC 2 cut(s) 258, 2165
Hsp92II CATG 8 cut(s) 1130, 1268, 1349, 1378, 1535, 1633, 1828, 1840
HspAI GCGC 5 cut(s) 298, 383, 385, 430, 2425
Kpn2I TCCGGA 1 cut(s) 871
Ksp22I TGATCA 2 cut(s) 579, 1947
Kzo9I GATC 4 cut(s) 202, 579, 1947, 2260
LguI GCTCTTC 1 cut(s) 40
LmnI GCTCC 5 cut(s) 411, 940, 1150, 1780, 2318
Lsp1109I GCAGC 7 cut(s) 409, 439, 486, 567, 1079, 1205, 2332
LweI GCATC 9 cut(s) 315, 1050, 1180, 1694, 1830, 1867, 1890, 2012, 2563
MaeI CTAG 3 cut(s) 537, 1004, 1398
MaeII ACGT 6 cut(s) 258, 986, 1199, 1304, 1683, 2498
MaeIII GTNAC 9 cut(s) 94, 226, 596, 775, 890, 999, 1252, 1862, 2504
MalI GATC 4 cut(s) 204, 581, 1949, 2262
MauBI CGCGCGCG 1 cut(s) 383
MboI GATC 4 cut(s) 202, 579, 1947, 2260
MboII GAAGA 6 cut(s) 27, 406, 553, 652, 1676, 2311
MfeI CAATTG 1 cut(s) 1019
MflI RGATCY 2 cut(s) 202, 2260
MhlI GDGCHC 7 cut(s) 123, 459, 515, 687, 1293, 1785, 2181
MlyI GAGTC 5 cut(s) 104, 942, 1046, 1132, 1964
MmeI TCCRAC 1 cut(s) 690
Mph1103I ATGCAT 1 cut(s) 1537
MroI TCCGGA 1 cut(s) 871
MroXI GAANNNNTTC 2 cut(s) 353, 2142
MslI CAYNNNNRTG 5 cut(s) 470, 518, 1217, 1383, 2068
MspA1I CMGCKG 2 cut(s) 400, 2122
MspI CCGG 6 cut(s) 107, 124, 159, 767, 872, 2526
MspR9I CCNGG 5 cut(s) 107, 187, 542, 1606, 2526
MunI CAATTG 1 cut(s) 1019
MvaI CCWGG 3 cut(s) 187, 542, 1606
MvnI CGCG 6 cut(s) 298, 300, 383, 385, 387, 492
NciI CCSGG 2 cut(s) 107, 2526
NdeI CATATG 2 cut(s) 1012, 1432
NdeII GATC 4 cut(s) 202, 579, 1947, 2260
NlaIII CATG 8 cut(s) 1130, 1268, 1349, 1378, 1535, 1633, 1828, 1840
NlaIV GGNNCC 4 cut(s) 218, 317, 1724, 2476
NmeAIII GCCGAG 2 cut(s) 465, 684
NmuCI GTSAC 4 cut(s) 94, 226, 1252, 1862
NsiI ATGCAT 1 cut(s) 1537
NspI RCATGY 2 cut(s) 1378, 1535
OliI CACNNNNGTG 2 cut(s) 518, 2068
PagI TCATGA 2 cut(s) 1264, 1345
PauI GCGCGC 1 cut(s) 383
PceI AGGCCT 1 cut(s) 1409
PciSI GCTCTTC 1 cut(s) 40
PcsI WCGNNNNNNNCGW 1 cut(s) 606
PdmI GAANNNNTTC 2 cut(s) 353, 2142
PfeI GAWTC 4 cut(s) 129, 1111, 1352, 2443
PflMI CCANNNNNTGG 1 cut(s) 547
PfoI TCCNGGA 1 cut(s) 2524
PleI GAGTC 5 cut(s) 104, 941, 1045, 1131, 1964
PpsI GAGTC 5 cut(s) 104, 941, 1045, 1131, 1964
Ppu21I YACGTR 1 cut(s) 1200
PsiI TTATAA 2 cut(s) 675, 783
Psp6I CCWGG 3 cut(s) 185, 540, 1604
PspFI CCCAGC 1 cut(s) 2074
PspGI CCWGG 3 cut(s) 185, 540, 1604
PspN4I GGNNCC 4 cut(s) 218, 317, 1724, 2476
PspPI GGNCC 4 cut(s) 550, 769, 1722, 2475
PsrI GAACNNNNNNTAC 2 cut(s) 2228, 2260
PstI CTGCAG 1 cut(s) 2364
PsuI RGATCY 2 cut(s) 202, 2260
PteI GCGCGC 1 cut(s) 383
RsaI GTAC 5 cut(s) 248, 608, 1118, 1402, 2292
RsaNI GTAC 5 cut(s) 247, 607, 1117, 1401, 2291
RseI CAYNNNNRTG 5 cut(s) 470, 518, 1217, 1383, 2068
SapI GCTCTTC 1 cut(s) 40
Sau3AI GATC 4 cut(s) 202, 579, 1947, 2260
Sau96I GGNCC 4 cut(s) 550, 769, 1722, 2475
SchI GAGTC 5 cut(s) 104, 942, 1046, 1132, 1964
ScrFI CCNGG 5 cut(s) 107, 187, 542, 1606, 2526
SduI GDGCHC 7 cut(s) 123, 459, 515, 687, 1293, 1785, 2181
SfaNI GCATC 9 cut(s) 315, 1050, 1180, 1694, 1830, 1867, 1890, 2012, 2563
SfcI CTRYAG 1 cut(s) 2360
SinI GGWCC 4 cut(s) 550, 769, 1722, 2475
SmiMI CAYNNNNRTG 5 cut(s) 470, 518, 1217, 1383, 2068
SmlI CTYRAG 4 cut(s) 926, 1160, 1727, 2435
SmoI CTYRAG 4 cut(s) 926, 1160, 1727, 2435
SnaBI TACGTA 1 cut(s) 1200
SseBI AGGCCT 1 cut(s) 1409
SspI AATATT 2 cut(s) 1812, 1924
SspMI CTAG 3 cut(s) 537, 1004, 1398
StuI AGGCCT 1 cut(s) 1409
StyD4I CCNGG 5 cut(s) 105, 185, 540, 1604, 2524
StyI CCWWGG 1 cut(s) 1404
TaiI ACGT 6 cut(s) 261, 989, 1202, 1307, 1686, 2501
TaqI TCGA 5 cut(s) 261, 321, 525, 1028, 2410
TatI WGTACW 1 cut(s) 246
TauI GCSGC 5 cut(s) 383, 492, 751, 1091, 1445
TfiI GAWTC 4 cut(s) 129, 1111, 1352, 2443
TscAI CASTG 4 cut(s) 94, 1188, 1825, 2551
TseFI GTSAC 4 cut(s) 94, 226, 1252, 1862
TseI GCWGC 7 cut(s) 397, 427, 499, 555, 1067, 1193, 2345
Tsp45I GTSAC 4 cut(s) 94, 226, 1252, 1862
TspGWI ACGGA 3 cut(s) 106, 127, 2502
TspRI CASTG 4 cut(s) 94, 1188, 1825, 2551
Van91I CCANNNNNTGG 1 cut(s) 547
VneI GTGCAC 3 cut(s) 119, 511, 2177
VpaK11BI GGWCC 4 cut(s) 550, 769, 1722, 2475
XapI RAATTY 3 cut(s) 623, 970, 1362
XceI RCATGY 2 cut(s) 1378, 1535
XcmI CCANNNNNNNNNTGG 1 cut(s) 1622
XmnI GAANNNNTTC 2 cut(s) 353, 2142
XspI CTAG 3 cut(s) 537, 1004, 1398
ZraI GACGTC 1 cut(s) 259
Zsp2I ATGCAT 1 cut(s) 1537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.