MD14G1189200.v1.1

Pentatricopeptide repeat-containing protein At1g74850

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009497
Physical Location & Seq
Forward (+)
28075613 .. 28080272
4660 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1189200.v1.1.491

Sequence Viewer

Length: 2976 bp
ATGGATTATGATAAAGGATCTGGAGTTCCGTTAAGGGAAAAGGGAGCTTTGATATGCGTGCGTGGTGCGACAGTTGGATGGAGGCGATTGCGGGTCTGCTTACTTACTGTGGGCAAGGTTCGGGTGGATAACCCGTTCGAATTGGACCCGACCCACTATCTCTACTCGCCTTCTTCCCCCTATTATCCACAACCGCTTGCACGTGTCCTTCTTCAAGCTAAGTCTTCTCCCACCCGCTCTCCCCTTCTGTTATCTCCTGTAACTTTCTCGAGAAAATCACAGAGAAAATCCAATCCGAAGCTCCGAGAGGCTCATGCGAGCCTAATTACCGAAATGTCCTTCTCCCCCACGCTCTCCATTTCCAGCTCCTCGCCTCTCTCCACCTCAATTTCCAAAATTAACCCCAAGTTCAACCACCTCACCGTCCTCACCAACTCATCAACCCACCGGAGATTCCTCTCCGGTCGCACGACCTTTCTCTCCGGCCGCACGACCTTCCTCTCCGGCGACCGCCGGCTGGAGTTCTCGGCCAGGGCGAAGCCGAAGGACCTCATCCTCGGGAACCCGTCGGTGACGCTGGAGAAGGGGAAGTACAGCTACGACGTCGAAACCCTAATCAACAAGCTCAGCAGCCTCCCTCCACGCGGCAGCATCGCTCGGTGCCTCGACATCTTCAAGAACAAGCTGTCACTAAACGACTTCGCTTTGGTGTTCAAGGAGTTCGCCGCGCGCGGTGATTGGCAGCGGTCGCTCCGCCTCTTCAAGTATATGCAGCGCCAGATATGGTGCAAGCCGAACGAGCACATATACACGATAATGATCAGCTTGCTCGGCCGCGAGGGGCTGCTGGATAAGTGCGCCGAGGTGTTCGACGAAATGCCTAGCCAGGGCGTGGTCCGCAGCGTCTTCAGCTACACCGCATTGATCAATGCCTACGGGCGTAACGGTCAGTATGAAACATCCCTTGAATTTCTTGATCGAATGAAGAAAGATAAGGTTCCGCCGAGTATTTTGACTTACAATACTGTGCTCAATGCCTGTGCTAGAGGTGGGCTGGATTGGGAAGGGTTATTAGGATTGTTTGCCGAAATGCGGCATGAAGGAATACAGCCTGACCTTGTTACTTATAATACTTTGCTTAGTGCTTGTGCTGGTAGAGGGTTAGGCGATGAGGCAGAGATGGTGTTCAGGACTATGAATGAGGGTGGGATTGTTCCGGATATGACCACTTATCGTTACCTTGTTGAAACTTTTGGTAAATTGGACAAGCTCGAGAAAGTCTCGGAGCTTCTTAAAGAGATGGAAGCTGGAGGGAATTTGCCTGAGATTACGTCGTATAATGTGTTACTGGAGGCATATGCTGAATTGGGGTCGATTAGAGAGTCAATGGGTGTGTTTAGGCAGATGCAGGCTGCGGGGTGTATGCCGAATGCTGCTACTTATAGTATTTTGTTGAATTTGTATGGGAGGCATGGAAGGTATGATGATGTTCGAGAGCTTTTTCTTGAGATGAAAGTGAGCAATACGGAGCCAGATGCGGCTACATATAACATTCTCATACAGGTATTTGGGGAGGGTGGGTATTTTAAGGAAGTAGTGACTTTGTTTCATGATATGGTGGAGGAGAAAATCGAGCCGAATATGGAGACGTATGAAGGGTTGATATATGCTTGTGGAAAGGGAGGACTCCATGAGGATTCCAAGAAAATTTTACTTCACATGCACGAGAATGGAATAGTGCCTAGTACCAAGGCCTATACAGGGGTTATTGAAGCATGTGGGCAAGCGGCATTGTATGATGAAGCTCTTGTTGCCTTCAACACAATGAATGAAGTGGGAAGCAAACCATCAGTTGAAAGCTACAACTCGCTGATATATGCGTTTGCAAGAGGCGGACTATACAGGGAGACTGAAGCAGTCTTGTTAATAATGGGCAAGGCTGGTGTTGCCAGGAATGTCCATACAGTCAATGGCATGATAGAAGCTTTTAGGCAAGGAGGTCAATTTGAAGAAGCTATCAAGGCGTACGTTGAGATGGAAAAGACAAGATGCGATCCCGATGAGTGGACCCTTGAGGCAGTTTTAAGTGTTTACTGCGTTGCAGGTCTTGTTAATGAGTGTGAGGAGCACTTCCAAGAGATTAAAGCCTCAGGAATATTACCCAGTGTTATGTGCTACTGCATGATGCTTGCTGTTTATGCAAGGAGTAACAGGTGGGATGATGCCTATGTGTTGCTGAATGAGATGCTTACAAACAGGGCATCGAATATTCATCAAGTGATTGGGCAGATGATCAAGGGAGATTATGATGATGACTCTAACTGGCAGATGGTAGAGTATGTTTTTGACAAATTAAAATCTGAAGGATGCGGCTTGGGAATGAGGTTCTACAACACACTACTAGAAGCACTTTGGTGGCTGGGTCAGAAACAAAGAGCTGCGCGAGTTCTTAATGAAGCAACACAGCGGGGGCTTTTTCCTGAACTTTTTCGTAAAAATAAACTTGTGGTGTCTGTTGATGTGCACAGAATGTGGCAAGGCGGTGCGTATACAGCAATGTCAGTTTGGCTAAACAATATGTATGAGATGTTCCTCAATGGCGAGGATCTACCCCATCTTGCAACTGTCGTTGTAGTACGAGGGAAGATGGAGAAAAGCTCAATAACACAAGATCTACCGATCGCAAAGTCTGCTTATACATTTCTGCAGGATCATATGCCAGCTTCCTTTTCTTTCCCAAAATGGAACAATGGTCGAATTCTCTGTCAGCGCCCTCAGCTCAAGCGGATCCTATCAAGCATCGAACCATCCACAGATGAGTCCGAAAGGAATAATATTATCACGTTAAGCAACTCCCCGTTTCCTCCTCCAGGAACCAAGACATCCTCCAGTGCCGTCAATAGCCGACGGTATAATGATGCCGGTTCTGATGAAAGAATTAGGACAAGAACGGAGGTTTTGACAAGCATGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

992

Amino Acids

111.45

Weight (kDa)

7.22

Isoelectric Point (pI)

42.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 248 - 351 1e-05 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 265 - 310 6.6e-08 PPR repeat family
PPR PF01535 269 - 298 4.8e-06 PPR repeat
TPR_24 PF23276 278 - 384 9.6e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 289 - 347 6.3e-14 Pentatricopeptide repeat domain
PPR_2 PF13041 301 - 349 8e-13 PPR repeat family
PPR PF01535 303 - 332 6.4e-07 PPR repeat
PPR_long PF17177 316 - 458 1.3e-11 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 342 - 469 3.6e-09 Fungal tetratrico peptide repeats
PPR_2 PF13041 358 - 384 4.7e-06 PPR repeat family
PPR_3 PF13812 360 - 420 6.9e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 368 - 399 7.7e-06 PPR repeat
PPR_2 PF13041 371 - 418 1.6e-10 PPR repeat family
PPR_3 PF13812 430 - 489 5e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 441 - 486 2.6e-10 PPR repeat family
PPR PF01535 480 - 507 7.2e-06 PPR repeat
PPR_2 PF13041 483 - 523 2.9e-09 PPR repeat family
PPR_3 PF13812 500 - 560 1.6e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 515 - 560 6.8e-09 PPR repeat family
PPR_3 PF13812 535 - 595 1.2e-06 Pentatricopeptide repeat domain
PPR_3 PF13812 570 - 628 6.1e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 651 - 699 3.5e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1128
AatII GACGTC 1 cut(s) 606
Acc36I ACCTGC 1 cut(s) 2093
AccB1I GGYRCC 1 cut(s) 660
AccB7I CCANNNNNTGG 1 cut(s) 892
AccBSI CCGCTC 1 cut(s) 237
AccI GTMKAC 1 cut(s) 2549
AccII CGCG 6 cut(s) 645, 728, 730, 732, 837, 2443
AccIII TCCGGA 1 cut(s) 1216
AclWI GGATC 6 cut(s) 25, 2048, 2613, 2718, 2782, 2795
AcoI YGGCCR 3 cut(s) 484, 528, 832
AcsI RAATTY 5 cut(s) 968, 1315, 1456, 1707, 2757
AcuI CTGAAG 3 cut(s) 892, 1932, 2382
AcvI CACGTG 1 cut(s) 203
AcyI GRCGYC 1 cut(s) 603
AfaI GTAC 4 cut(s) 593, 1747, 2027, 2637
AfiI CCNNNNNNNGG 8 cut(s) 517, 644, 887, 892, 1092, 1761, 2064, 2870
AflIII ACRYGT 1 cut(s) 202
AjnI CCWGG 4 cut(s) 530, 885, 1949, 2869
AleI CACNNNNGTG 1 cut(s) 2413
AloI GAACNNNNNNTCC 4 cut(s) 9, 41, 119, 151
Alw21I GWGCWC 4 cut(s) 804, 1032, 2130, 2526
Alw26I GTCTC 3 cut(s) 1285, 1640, 1901
Alw44I GTGCAC 1 cut(s) 2522
AlwI GGATC 6 cut(s) 25, 2048, 2613, 2718, 2782, 2795
Ama87I CYCGRG 3 cut(s) 268, 557, 1271
Aor13HI TCCGGA 1 cut(s) 1216
AoxI GGCC 4 cut(s) 484, 528, 832, 1752
ApaLI GTGCAC 1 cut(s) 2522
ApeKI GCWGC 9 cut(s) 630, 648, 742, 772, 844, 900, 1412, 1433, 2438
ApoI RAATTY 5 cut(s) 968, 1315, 1456, 1707, 2757
ArsI GACNNNNNNTTYG 2 cut(s) 2749, 2781
Asp700I GAANNNNTTC 1 cut(s) 2487
AspLEI GCGC 6 cut(s) 730, 732, 777, 860, 2443, 2772
AspS9I GGNCC 4 cut(s) 145, 547, 895, 2067
AsuHPI GGTGA 4 cut(s) 412, 421, 583, 746
AsuII TTCGAA 1 cut(s) 138
AvaI CYCGRG 3 cut(s) 268, 557, 1271
AvaII GGWCC 4 cut(s) 145, 547, 895, 2067
AxyI CCTNAGG 1 cut(s) 2149
BaeGI GKGCMC 1 cut(s) 2526
BamHI GGATCC 1 cut(s) 2787
BanI GGYRCC 1 cut(s) 660
BarI GAAGNNNNNNTAC 4 cut(s) 575, 581, 607, 613
BauI CACGAG 1 cut(s) 1724
BbrPI CACGTG 1 cut(s) 203
BbsI GAAGAC 2 cut(s) 216, 898
Bbv12I GWGCWC 4 cut(s) 804, 1032, 2130, 2526
BbvCI CCTCAGC 1 cut(s) 2775
BbvI GCAGC 9 cut(s) 642, 660, 754, 784, 831, 912, 1399, 1420, 2425
BccI CCATC 9 cut(s) 72, 1174, 1294, 1855, 2029, 2323, 2622, 2641, 2815
BceAI ACGGC 1 cut(s) 2879
BciT130I CCWGG 4 cut(s) 532, 887, 1951, 2871
BclI TGATCA 3 cut(s) 819, 924, 2292
BcoDI GTCTC 3 cut(s) 1285, 1640, 1901
BfaI CTAG 4 cut(s) 882, 1044, 1743, 2402
BfmI CTRYAG 1 cut(s) 2705
BfoI RGCGCY 2 cut(s) 778, 2773
BfuAI ACCTGC 1 cut(s) 2093
BglII AGATCT 1 cut(s) 2671
BlpI GCTNAGC 1 cut(s) 626
Bme1390I CCNGG 4 cut(s) 532, 887, 1951, 2871
Bme18I GGWCC 4 cut(s) 145, 547, 895, 2067
BmeT110I CYCGRG 3 cut(s) 268, 557, 1271
BmgT120I GGNCC 4 cut(s) 145, 547, 895, 2067
BmiI GGNNCC 8 cut(s) 147, 563, 662, 999, 1530, 2069, 2789, 2875
BmrFI CCNGG 4 cut(s) 532, 887, 1951, 2871
BmrI ACTGGG 1 cut(s) 2157
BmuI ACTGGG 1 cut(s) 2157
BpiI GAAGAC 2 cut(s) 216, 898
BplI GAGNNNNNCTC 4 cut(s) 1265, 1297, 2642, 2674
BpmI CTGGAG 7 cut(s) 42, 539, 599, 1329, 1370, 2853, 2872
Bpu10I CCTNAGC 1 cut(s) 2775
Bpu1102I GCTNAGC 1 cut(s) 626
Bpu14I TTCGAA 1 cut(s) 138
BpuEI CTTGAG 3 cut(s) 1526, 2093, 2765
BsaAI YACGTR 1 cut(s) 203
BsaBI GATNNNNATC 1 cut(s) 818
BsaHI GRCGYC 1 cut(s) 603
BsaJI CCNNGG 5 cut(s) 531, 556, 861, 886, 1749
BsaWI WCCGGW 3 cut(s) 447, 461, 1216
BsaXI ACNNNNNCTCC 4 cut(s) 223, 253, 1565, 1595
Bsc4I CCNNNNNNNGG 8 cut(s) 517, 644, 887, 892, 1092, 1761, 2064, 2870
Bse118I RCCGGY 2 cut(s) 513, 2921
Bse1I ACTGG 4 cut(s) 1353, 2163, 2327, 2889
Bse21I CCTNAGG 1 cut(s) 2149
Bse3DI GCAATG 1 cut(s) 2562
Bse8I GATNNNNATC 1 cut(s) 818
BseAI TCCGGA 1 cut(s) 1216
BseBI CCWGG 4 cut(s) 532, 887, 1951, 2871
BseDI CCNNGG 5 cut(s) 531, 556, 861, 886, 1749
BseGI GGATG 7 cut(s) 83, 552, 959, 2224, 2372, 2807, 2882
BseJI GATNNNNATC 1 cut(s) 818
BseLI CCNNNNNNNGG 8 cut(s) 517, 644, 887, 892, 1092, 1761, 2064, 2870
BseMI GCAATG 1 cut(s) 2562
BseMII CTCAG 4 cut(s) 640, 1314, 2163, 2789
BseNI ACTGG 4 cut(s) 1353, 2163, 2327, 2889
BsePI GCGCGC 1 cut(s) 728
BseRI GAGGAG 4 cut(s) 358, 1637, 2138, 2856
BseSI GKGCMC 1 cut(s) 2526
BseX3I CGGCCG 2 cut(s) 484, 832
BseXI GCAGC 9 cut(s) 642, 660, 754, 784, 831, 912, 1399, 1420, 2425
BseYI CCCAGC 1 cut(s) 2419
Bsh1236I CGCG 6 cut(s) 645, 728, 730, 732, 837, 2443
Bsh1285I CGRYCG 6 cut(s) 466, 487, 511, 749, 835, 2682
BshFI GGCC 4 cut(s) 486, 530, 834, 1754
BshNI GGYRCC 1 cut(s) 660
BsiEI CGRYCG 6 cut(s) 466, 487, 511, 749, 835, 2682
BsiHKAI GWGCWC 4 cut(s) 804, 1032, 2130, 2526
BsiHKCI CYCGRG 3 cut(s) 268, 557, 1271
BsiSI CCGG 7 cut(s) 448, 462, 483, 504, 514, 1217, 2922
BsiWI CGTACG 1 cut(s) 2025
BslI CCNNNNNNNGG 8 cut(s) 517, 644, 887, 892, 1092, 1761, 2064, 2870
BsmAI GTCTC 3 cut(s) 1285, 1640, 1901
BsmBI CGTCTC 1 cut(s) 1640
BsmI GAATGC 1 cut(s) 1435
BsnI GGCC 4 cut(s) 486, 530, 834, 1754
BsoBI CYCGRG 3 cut(s) 268, 557, 1271
Bsp119I TTCGAA 1 cut(s) 138
Bsp1286I GDGCHC 4 cut(s) 804, 1032, 2130, 2526
Bsp13I TCCGGA 1 cut(s) 1216
Bsp1720I GCTNAGC 1 cut(s) 626
BspANI GGCC 4 cut(s) 486, 530, 834, 1754
BspCNI CTCAG 4 cut(s) 639, 1315, 2162, 2788
BspEI TCCGGA 1 cut(s) 1216
BspFNI CGCG 6 cut(s) 645, 728, 730, 732, 837, 2443
BspHI TCATGA 1 cut(s) 1609
BspLI GGNNCC 8 cut(s) 147, 563, 662, 999, 1530, 2069, 2789, 2875
BspMAI CTGCAG 1 cut(s) 2709
BspMI ACCTGC 1 cut(s) 2093
BspPI GGATC 6 cut(s) 25, 2048, 2613, 2718, 2782, 2795
BspT104I TTCGAA 1 cut(s) 138
BspT107I GGYRCC 1 cut(s) 660
BsrBI CCGCTC 1 cut(s) 237
BsrDI GCAATG 1 cut(s) 2562
BsrFI RCCGGY 2 cut(s) 513, 2921
BsrI ACTGG 4 cut(s) 1353, 2163, 2327, 2889
BssAI RCCGGY 2 cut(s) 513, 2921
BssECI CCNNGG 5 cut(s) 531, 556, 861, 886, 1749
BssHII GCGCGC 1 cut(s) 728
BssNAI GTATAC 1 cut(s) 2550
BssNI GRCGYC 1 cut(s) 603
BssSI CACGAG 1 cut(s) 1724
BssT1I CCWWGG 1 cut(s) 1749
Bst1107I GTATAC 1 cut(s) 2550
Bst2BI CACGAG 1 cut(s) 1724
Bst2UI CCWGG 4 cut(s) 532, 887, 1951, 2871
Bst4CI ACNGT 8 cut(s) 73, 109, 424, 947, 1027, 1966, 2626, 2910
Bst6I CTCTTC 1 cut(s) 764
BstACI GRCGYC 1 cut(s) 603
BstAPI GCANNNNNTGC 1 cut(s) 2690
BstBAI YACGTR 1 cut(s) 203
BstBI TTCGAA 1 cut(s) 138
BstDEI CTNAG 6 cut(s) 219, 626, 1139, 1323, 2149, 2775
BstENI CCTNNNNNAGG 1 cut(s) 2868
BstF5I GGATG 7 cut(s) 83, 552, 959, 2224, 2372, 2807, 2882
BstFNI CGCG 6 cut(s) 645, 728, 730, 732, 837, 2443
BstH2I RGCGCY 2 cut(s) 778, 2773
BstHHI GCGC 6 cut(s) 730, 732, 777, 860, 2443, 2772
BstMAI GTCTC 3 cut(s) 1285, 1640, 1901
BstMCI CGRYCG 6 cut(s) 466, 487, 511, 749, 835, 2682
BstNI CCWGG 4 cut(s) 532, 887, 1951, 2871
BstNSI RCATGY 2 cut(s) 1723, 1779
BstSCI CCNGG 4 cut(s) 530, 885, 1949, 2869
BstSFI CTRYAG 1 cut(s) 2705
BstSLI GKGCMC 1 cut(s) 2526
BstUI CGCG 6 cut(s) 645, 728, 730, 732, 837, 2443
BstV1I GCAGC 9 cut(s) 642, 660, 754, 784, 831, 912, 1399, 1420, 2425
BstV2I GAAGAC 2 cut(s) 216, 898
BstX2I RGATCY 4 cut(s) 17, 2605, 2671, 2787
BstYI RGATCY 4 cut(s) 17, 2605, 2671, 2787
BstZ17I GTATAC 1 cut(s) 2550
BstZI CGGCCG 2 cut(s) 484, 832
Bsu36I CCTNAGG 1 cut(s) 2149
BsuRI GGCC 4 cut(s) 486, 530, 834, 1754
BtgZI GCGATG 2 cut(s) 637, 1182
BtsCI GGATG 7 cut(s) 83, 552, 959, 2224, 2372, 2807, 2882
BtsIMutI CAGTG 2 cut(s) 2170, 2896
BveI ACCTGC 1 cut(s) 2093
CciI TCATGA 1 cut(s) 1609
CfoI GCGC 6 cut(s) 730, 732, 777, 860, 2443, 2772
Cfr10I RCCGGY 2 cut(s) 513, 2921
Cfr13I GGNCC 4 cut(s) 145, 547, 895, 2067
CseI GACGC 2 cut(s) 583, 892
Csp6I GTAC 4 cut(s) 592, 1746, 2026, 2636
CviQI GTAC 4 cut(s) 592, 1746, 2026, 2636
DdeI CTNAG 6 cut(s) 219, 626, 1139, 1323, 2149, 2775
EaeI YGGCCR 3 cut(s) 484, 528, 832
EagI CGGCCG 2 cut(s) 484, 832
Eam1104I CTCTTC 1 cut(s) 764
EarI CTCTTC 1 cut(s) 764
EciI GGCGGA 3 cut(s) 743, 990, 1908
EclXI CGGCCG 2 cut(s) 484, 832
Eco130I CCWWGG 1 cut(s) 1749
Eco147I AGGCCT 1 cut(s) 1754
Eco47I GGWCC 4 cut(s) 145, 547, 895, 2067
Eco52I CGGCCG 2 cut(s) 484, 832
Eco57I CTGAAG 3 cut(s) 892, 1932, 2382
Eco72I CACGTG 1 cut(s) 203
Eco81I CCTNAGG 1 cut(s) 2149
Eco88I CYCGRG 3 cut(s) 268, 557, 1271
EcoNI CCTNNNNNAGG 1 cut(s) 2868
EcoO109I RGGNCCY 1 cut(s) 547
EcoRI GAATTC 1 cut(s) 2757
EcoRII CCWGG 4 cut(s) 530, 885, 1949, 2869
EcoT14I CCWWGG 1 cut(s) 1749
ErhI CCWWGG 1 cut(s) 1749
Esp3I CGTCTC 1 cut(s) 1640
FauI CCCGC 4 cut(s) 84, 242, 1408, 2460
FauNDI CATATG 2 cut(s) 1357, 2715
FbaI TGATCA 3 cut(s) 819, 924, 2292
FblI GTMKAC 1 cut(s) 2549
FokI GGATG 7 cut(s) 90, 539, 946, 2231, 2379, 2794, 2869
FspBI CTAG 4 cut(s) 882, 1044, 1743, 2402
GlaI GCGC 6 cut(s) 729, 731, 776, 859, 2442, 2771
GsaI CCCAGC 1 cut(s) 2423
GsuI CTGGAG 7 cut(s) 42, 539, 599, 1329, 1370, 2853, 2872
HaeII RGCGCY 2 cut(s) 778, 2773
HaeIII GGCC 4 cut(s) 486, 530, 834, 1754
HapII CCGG 7 cut(s) 448, 462, 483, 504, 514, 1217, 2922
HgaI GACGC 2 cut(s) 583, 892
HhaI GCGC 6 cut(s) 730, 732, 777, 860, 2443, 2772
Hin1I GRCGYC 1 cut(s) 603
Hin6I GCGC 6 cut(s) 728, 730, 775, 858, 2441, 2770
HinP1I GCGC 6 cut(s) 728, 730, 775, 858, 2441, 2770
HindIII AAGCTT 1 cut(s) 1983
HinfI GANTC 6 cut(s) 453, 1382, 1686, 1697, 2315, 2819
HpaII CCGG 7 cut(s) 448, 462, 483, 504, 514, 1217, 2922
HphI GGTGA 4 cut(s) 412, 421, 583, 746
Hpy166II GTNNAC 4 cut(s) 2067, 2092, 2524, 2550
Hpy188I TCNGA 7 cut(s) 297, 305, 1285, 2362, 2427, 2824, 2929
Hpy8I GTNNAC 4 cut(s) 2067, 2092, 2524, 2550
Hpy99I CGWCG 6 cut(s) 571, 605, 608, 875, 1336, 2910
HpyCH4III ACNGT 8 cut(s) 73, 109, 424, 947, 1027, 1966, 2626, 2910
HpyCH4IV ACGT 6 cut(s) 202, 603, 1331, 1649, 2028, 2843
HpyF3I CTNAG 6 cut(s) 219, 626, 1139, 1323, 2149, 2775
HpySE526I ACGT 6 cut(s) 202, 603, 1331, 1649, 2028, 2843
Hsp92I GRCGYC 1 cut(s) 603
HspAI GCGC 6 cut(s) 728, 730, 775, 858, 2441, 2770
Kpn2I TCCGGA 1 cut(s) 1216
KroI GCCGGC 1 cut(s) 513
KroNI GCCGGC 1 cut(s) 515
Ksp22I TGATCA 3 cut(s) 819, 924, 2292
LmnI GCTCC 7 cut(s) 44, 306, 371, 756, 1285, 1528, 2125
Lsp1109I GCAGC 9 cut(s) 642, 660, 754, 784, 831, 912, 1399, 1420, 2425
MaeI CTAG 4 cut(s) 882, 1044, 1743, 2402
MaeII ACGT 6 cut(s) 202, 603, 1331, 1649, 2028, 2843
MaeIII GTNAC 9 cut(s) 259, 571, 687, 941, 1120, 1235, 1344, 1597, 2207
MauBI CGCGCGCG 1 cut(s) 728
MbiI CCGCTC 1 cut(s) 237
MboII GAAGA 9 cut(s) 165, 203, 216, 664, 751, 898, 997, 2021, 2656
MflI RGATCY 4 cut(s) 17, 2605, 2671, 2787
MhlI GDGCHC 4 cut(s) 804, 1032, 2130, 2526
MlyI GAGTC 4 cut(s) 1391, 1680, 2309, 2828
MmeI TCCRAC 1 cut(s) 55
MroI TCCGGA 1 cut(s) 1216
MroNI GCCGGC 1 cut(s) 513
MroXI GAANNNNTTC 1 cut(s) 2487
MslI CAYNNNNRTG 3 cut(s) 815, 1728, 2413
MspA1I CMGCKG 2 cut(s) 745, 2467
MspI CCGG 7 cut(s) 448, 462, 483, 504, 514, 1217, 2922
MspR9I CCNGG 4 cut(s) 532, 887, 1951, 2871
Mva1269I GAATGC 1 cut(s) 1435
MvaI CCWGG 4 cut(s) 532, 887, 1951, 2871
MvnI CGCG 6 cut(s) 645, 728, 730, 732, 837, 2443
NaeI GCCGGC 1 cut(s) 515
NdeI CATATG 2 cut(s) 1357, 2715
NgoMIV GCCGGC 1 cut(s) 513
NlaIV GGNNCC 8 cut(s) 147, 563, 662, 999, 1530, 2069, 2789, 2875
NmeAIII GCCGAG 4 cut(s) 506, 810, 886, 1029
NmuCI GTSAC 3 cut(s) 571, 687, 1597
NspI RCATGY 2 cut(s) 1723, 1779
NspV TTCGAA 1 cut(s) 138
OliI CACNNNNGTG 1 cut(s) 2413
PaeR7I CTCGAG 2 cut(s) 268, 1271
PagI TCATGA 1 cut(s) 1609
PauI GCGCGC 1 cut(s) 728
PceI AGGCCT 1 cut(s) 1754
PcsI WCGNNNNNNNCGW 1 cut(s) 533
PctI GAATGC 1 cut(s) 1435
PdiI GCCGGC 1 cut(s) 515
PdmI GAANNNNTTC 1 cut(s) 2487
PfeI GAWTC 2 cut(s) 453, 1697
Pfl23II CGTACG 1 cut(s) 2025
PflMI CCANNNNNTGG 1 cut(s) 892
PfoI TCCNGGA 1 cut(s) 2869
Ple19I CGATCG 1 cut(s) 2682
PleI GAGTC 4 cut(s) 1390, 1680, 2309, 2827
PmaCI CACGTG 1 cut(s) 203
PmlI CACGTG 1 cut(s) 203
PpsI GAGTC 4 cut(s) 1390, 1680, 2309, 2827
Ppu21I YACGTR 1 cut(s) 203
PpuMI RGGWCCY 1 cut(s) 547
PsiI TTATAA 1 cut(s) 1128
Psp5II RGGWCCY 1 cut(s) 547
Psp6I CCWGG 4 cut(s) 530, 885, 1949, 2869
PspCI CACGTG 1 cut(s) 203
PspFI CCCAGC 1 cut(s) 2419
PspGI CCWGG 4 cut(s) 530, 885, 1949, 2869
PspLI CGTACG 1 cut(s) 2025
PspN4I GGNNCC 8 cut(s) 147, 563, 662, 999, 1530, 2069, 2789, 2875
PspPI GGNCC 4 cut(s) 145, 547, 895, 2067
PspPPI RGGWCCY 1 cut(s) 547
PsrI GAACNNNNNNTAC 2 cut(s) 2573, 2605
PstI CTGCAG 1 cut(s) 2709
PsuI RGATCY 4 cut(s) 17, 2605, 2671, 2787
PteI GCGCGC 1 cut(s) 728
PvuI CGATCG 1 cut(s) 2682
RsaI GTAC 4 cut(s) 593, 1747, 2027, 2637
RsaNI GTAC 4 cut(s) 592, 1746, 2026, 2636
RseI CAYNNNNRTG 3 cut(s) 815, 1728, 2413
Sau96I GGNCC 4 cut(s) 145, 547, 895, 2067
SchI GAGTC 4 cut(s) 1391, 1680, 2309, 2828
ScrFI CCNGG 4 cut(s) 532, 887, 1951, 2871
SduI GDGCHC 4 cut(s) 804, 1032, 2130, 2526
SfcI CTRYAG 1 cut(s) 2705
Sfr274I CTCGAG 2 cut(s) 268, 1271
SfuI TTCGAA 1 cut(s) 138
SinI GGWCC 4 cut(s) 145, 547, 895, 2067
SlaI CTCGAG 2 cut(s) 268, 1271
SmiMI CAYNNNNRTG 3 cut(s) 815, 1728, 2413
SmlI CTYRAG 5 cut(s) 268, 1271, 1505, 2072, 2780
SmoI CTYRAG 5 cut(s) 268, 1271, 1505, 2072, 2780
SseBI AGGCCT 1 cut(s) 1754
SspI AATATT 3 cut(s) 2157, 2269, 2836
SspMI CTAG 4 cut(s) 882, 1044, 1743, 2402
StuI AGGCCT 1 cut(s) 1754
StyD4I CCNGG 4 cut(s) 530, 885, 1949, 2869
StyI CCWWGG 1 cut(s) 1749
TaaI ACNGT 8 cut(s) 73, 109, 424, 947, 1027, 1966, 2626, 2910
TaiI ACGT 6 cut(s) 205, 606, 1334, 1652, 2031, 2846
TatI WGTACW 1 cut(s) 591
TauI GCSGC 8 cut(s) 489, 648, 728, 837, 1096, 1541, 1790, 2373
TfiI GAWTC 2 cut(s) 453, 1697
TscAI CASTG 2 cut(s) 2170, 2896
TseFI GTSAC 3 cut(s) 571, 687, 1597
TseI GCWGC 9 cut(s) 630, 648, 742, 772, 844, 900, 1412, 1433, 2438
Tsp45I GTSAC 3 cut(s) 571, 687, 1597
TspGWI ACGGA 3 cut(s) 18, 1541, 2966
TspRI CASTG 2 cut(s) 2170, 2896
Van91I CCANNNNNTGG 1 cut(s) 892
VneI GTGCAC 1 cut(s) 2522
VpaK11BI GGWCC 4 cut(s) 145, 547, 895, 2067
XagI CCTNNNNNAGG 1 cut(s) 2868
XapI RAATTY 5 cut(s) 968, 1315, 1456, 1707, 2757
XceI RCATGY 2 cut(s) 1723, 1779
XcmI CCANNNNNNNNNTGG 1 cut(s) 1967
XhoI CTCGAG 2 cut(s) 268, 1271
XmiI GTMKAC 1 cut(s) 2549
XmnI GAANNNNTTC 1 cut(s) 2487
XspI CTAG 4 cut(s) 882, 1044, 1743, 2402
ZraI GACGTC 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.