Rh7DG030100

Pentatricopeptide repeat-containing protein At1g74850

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0009497
Physical Location & Seq
Forward (+)
2238227 .. 2242566
4340 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG030100.1

Sequence Viewer

Length: 2262 bp
ATGACCCTCTCCATTTCCATCCCCTCCCCTCTCTCCGCCCCAATCCTCAAGCTAAACCCTAAACCCCACCACCTCTCCTCCTTCCGATTCCTCTCCGGCCACCGCAAGTTCCTCCACGGCCAGCGGTTATCGGTCTCCGTCAGGGCGAAGCCGAAGGACCTCATCCTCGGGAACCCCTCCGTCACCGTCGAGAAGGGCAAGTACAGCTACGACGTCGAAACCCTAATCAACAAGCTCAGCAGCCTCCCGCCACGTGGCAGCATCGCGCGGTGCCTGGACATCTTCAAGAACAAGCTCTCCCTGAACGACTTCGCCCTGGTGTTCAAGGAGTTCGCGGCGCGTGGCGATTGGCAGAGGTCGCTGCGGCTGTTCAAGTATATGCAGCGGCAGATATGGTGCAAGCCGAGCGAGCACATCTACACCATCATGATCAGCCTGCTCGGCCGCGAAGGTTTACTTGACAAATGCGCTGAGATTTTCGACGAAATGCCTAGCCAAGGTGTGATTAGGACTGTGTTTAGCTACACTGCTTTGATTAATGCCTATGGCCGGAATGGTCAGTATGAAATGTCTCTTCAATTACTTGATAGAATGAAGAAAGATAGGGTTTCGCCGAATATATTGACTTATAATACTGTGCTTAATGCTTGTGCTAGAGGGGGGTTGGATTGGGAGGGGTTGTTAGGATTGTTTGCGGAAATGAGGCACGAGGGGGTTCAGCCGGACCTTGTTACTTATAATACATTGCTTAGTGCTTGCGCCGGGAGAGGTTTAGGTGATGAGGCGGAGATGGTGTTTAGGACTATGAATGAGGGTGGGATAGTTCCGGATATAACCACGTATAGTTATCTTGTTGAGACTTTTGGTAAATTGGATAAGCTTGAGAAGGTTTCCGAGTTGCTTAAAGGGATGGAGTCTGGGGGGAATTTGCCTGATATTACGTCGTATAATGTGTTGTTGGAGGCGTATGCGCAGTTGGGGTCGATTAAGGAGGCTATGGGTGTGTTCAGGCAGATGCAAGCGGCGGGGTGTATGCCGAATGCAGCTACTTATAGTATTTTGTTGAATTTGTATGGGAGGCATGGGAGGTATGATGATGTTCGGGAGCTTTTTCTTGAGATGAAAGTGAGCAATACGGAGCCAGATGCTGCTACGTATAATATTCTCATACAGGTGTTTGGTGAGGGTGGGTATTTTAGGGAGGTGGTGACTTTGTTTCATGATATGGTAGAGGAGAATATTGAGCCGAATATGGAGACGTATGAAGGATTGATATATGCTTGTGGAAAAGGAGGGCTCCATGAGGATGCCAAGAACATATTGCTTCACATGAATGAGAAGGGAATAGTGCCTAGTTCCAAGGCTTATACAGGAGTTATTGAAGCATATGGGCAAGCAGCACTGTATGATGAAGCTCTTGTTGCCTTCAACACAATGCATGAAGTGGGCAGCAGACCGTCAGTTGAAAGCTACAACTCGCTTATTCATGCATACGCAAGAGGCGGACTATACAAGGAGACTGAACAAATCTTGTCGATAATGGGTGAGGTTGGCATTGCAAGGAATGCCAGTTCATTCAATGGAATGATTGAAGCTTTTAGGCAGGGGGGTCAGTTTGAAGAAGCTATAAAGACGTACGTTGAGATGGAAAAGAGAAGATGTGATCCCGATGAGTGCACTCTTGAAGCAGTCTTAAGCGTTTACAGTGTTGCAGGTCTTGTTAATGAGTGTGAGGAGCACTTTGAAGAGATTAAAGCTTCAGGGATATTGCCCAGCGTCATGTGCTACTGCATGATGCTTGCTGTTTATGCAAAGACTGACAGGTGGGATGATGCCAATAAGTTGCTGAATGAGATGCTCACAAATAGGGTATCAAATATTCATCAAGTGATGGGACAGATGATAAAGGGAGATTATGATGACGAATCTAACTGGCAGATGGTAGAGTATGTTTTTGACAAACTAAAATCTGAAGGATGTGGGCTCGGAATGAGGTTCTACAACACTTTGATAGAAGCACTTTGGTGGCTGGGTCAGAAACAAAGAGCTGTGCGCGTTCTTAGTGAAGCAACTCAACGGGGGTTATTTCCTGAACTTCTCCGTAAAAATAAACTTGTTTGGTCTATAGATGTGCACAGGTATGCGGTAGAACTTGTCCTTTTAGGTCGTGTTCTCTTATTCGGGTTTATTTCAATTTTACCGGTGACCATTTTTGCAGGATGTGGGAAGGTGGTGCGTATGCAGCAATGTCACTTTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

753

Amino Acids

84.72

Weight (kDa)

5.91

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 117 - 220 6.6e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 135 - 179 1.1e-06 PPR repeat family
PPR PF01535 139 - 168 4.2e-06 PPR repeat
PPR_3 PF13812 159 - 217 5.6e-15 Pentatricopeptide repeat domain
PPR_2 PF13041 171 - 219 2.4e-14 PPR repeat family
PPR PF01535 173 - 202 9.5e-08 PPR repeat
PPR_long PF17177 191 - 328 8e-11 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 212 - 339 3.6e-09 Fungal tetratrico peptide repeats
PPR_2 PF13041 228 - 254 3.6e-06 PPR repeat family
PPR_3 PF13812 230 - 290 5.9e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 238 - 269 6.1e-06 PPR repeat
PPR_2 PF13041 241 - 288 2.3e-11 PPR repeat family
PPR_3 PF13812 300 - 359 1.3e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 311 - 356 3.7e-13 PPR repeat family
PPR PF01535 315 - 344 2.7e-06 PPR repeat
TPR_24 PF23276 315 - 407 1.6e-07 Fungal tetratrico peptide repeats
PPR PF01535 350 - 377 5.2e-06 PPR repeat
PPR_2 PF13041 353 - 393 2.1e-09 PPR repeat family
PPR_2 PF13041 385 - 430 2.7e-09 PPR repeat family
PPR_3 PF13812 405 - 465 5.3e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 451 - 500 3.2e-06 PPR repeat family
PPR_3 PF13812 478 - 533 3.4e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 486 - 533 9.7e-06 PPR repeat family
PPR_2 PF13041 522 - 565 1.9e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 630, 738
AatII GACGTC 1 cut(s) 216
Acc16I TGCGCA 1 cut(s) 972
Acc36I ACCTGC 1 cut(s) 1703
AccB1I GGYRCC 1 cut(s) 270
AccII CGCG 6 cut(s) 266, 268, 335, 340, 447, 2055
AccIII TCCGGA 1 cut(s) 826
AclWI GGATC 1 cut(s) 1658
AcoI YGGCCR 4 cut(s) 97, 118, 442, 547
AcsI RAATTY 2 cut(s) 925, 1066
AcuI CTGAAG 2 cut(s) 1743, 1992
AcvI CACGTG 1 cut(s) 254
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 2 cut(s) 203, 1637
AfiI CCNNNNNNNGG 3 cut(s) 254, 497, 549
AflII CTTAAG 1 cut(s) 1693
AgeI ACCGGT 1 cut(s) 2200
AjnI CCWGG 2 cut(s) 273, 315
AleI CACNNNNGTG 1 cut(s) 2023
AloI GAACNNNNNNTCC 4 cut(s) 281, 313, 1555, 1587
Alw21I GWGCWC 4 cut(s) 414, 1679, 1740, 2136
Alw26I GTCTC 5 cut(s) 139, 576, 851, 1250, 1511
Alw44I GTGCAC 2 cut(s) 1675, 2132
AlwI GGATC 1 cut(s) 1658
AlwNI CAGNNNCTG 1 cut(s) 1148
Ama87I CYCGRG 1 cut(s) 167
Aor13HI TCCGGA 1 cut(s) 826
AoxI GGCC 4 cut(s) 97, 118, 442, 547
ApaLI GTGCAC 2 cut(s) 1675, 2132
ApeKI GCWGC 9 cut(s) 240, 258, 361, 382, 1043, 1148, 1397, 1449, 2242
ApoI RAATTY 2 cut(s) 925, 1066
AseI ATTAAT 1 cut(s) 537
AsiGI ACCGGT 1 cut(s) 2200
Asp700I GAANNNNTTC 1 cut(s) 308
AspLEI GCGC 6 cut(s) 268, 340, 470, 761, 973, 2055
AspS9I GGNCC 2 cut(s) 157, 724
AsuC2I CCSGG 1 cut(s) 763
AsuHPI GGTGA 6 cut(s) 175, 788, 1193, 1219, 1556, 2215
AvaI CYCGRG 1 cut(s) 167
AvaII GGWCC 2 cut(s) 157, 724
BaeGI GKGCMC 2 cut(s) 1679, 2136
BanI GGYRCC 1 cut(s) 270
BanII GRGCYC 2 cut(s) 1299, 1986
BarI GAAGNNNNNNTAC 2 cut(s) 185, 217
BauI CACGAG 1 cut(s) 707
BbrPI CACGTG 1 cut(s) 254
Bbv12I GWGCWC 4 cut(s) 414, 1679, 1740, 2136
BbvI GCAGC 9 cut(s) 252, 270, 348, 394, 1055, 1135, 1409, 1461, 2254
BccI CCATC 7 cut(s) 26, 431, 784, 904, 1639, 1885, 1933
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 2 cut(s) 275, 317
BclI TGATCA 1 cut(s) 429
BcnI CCSGG 1 cut(s) 763
BcoDI GTCTC 5 cut(s) 139, 576, 851, 1250, 1511
BfaI CTAG 3 cut(s) 492, 654, 1353
BfmI CTRYAG 1 cut(s) 2124
BfrI CTTAAG 1 cut(s) 1693
BfuAI ACCTGC 1 cut(s) 1703
BglI GCCNNNNNGGC 1 cut(s) 441
BlpI GCTNAGC 1 cut(s) 236
Bme1390I CCNGG 3 cut(s) 275, 317, 763
Bme18I GGWCC 2 cut(s) 157, 724
BmeT110I CYCGRG 1 cut(s) 167
BmgT120I GGNCC 2 cut(s) 157, 724
BmiI GGNNCC 4 cut(s) 173, 272, 1140, 1298
BmrFI CCNGG 3 cut(s) 275, 317, 763
BmsI GCATC 7 cut(s) 270, 1005, 1135, 1297, 1785, 1822, 1845
Bpu1102I GCTNAGC 1 cut(s) 236
BpuEI CTTGAG 3 cut(s) 32, 902, 1136
BpuMI CCSGG 1 cut(s) 763
BsaAI YACGTR 3 cut(s) 254, 840, 1155
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 139
BsaJI CCNNGG 5 cut(s) 115, 166, 315, 496, 1359
BsaWI WCCGGW 2 cut(s) 826, 2200
Bsc4I CCNNNNNNNGG 3 cut(s) 254, 497, 549
Bse118I RCCGGY 1 cut(s) 2200
Bse1I ACTGG 2 cut(s) 1569, 1937
Bse3DI GCAATG 3 cut(s) 743, 1554, 2252
BseAI TCCGGA 1 cut(s) 826
BseBI CCWGG 2 cut(s) 275, 317
BseDI CCNNGG 5 cut(s) 115, 166, 315, 496, 1359
BseGI GGATG 7 cut(s) 18, 162, 915, 1312, 1834, 1982, 2225
BseLI CCNNNNNNNGG 3 cut(s) 254, 497, 549
BseMI GCAATG 3 cut(s) 743, 1554, 2252
BseMII CTCAG 2 cut(s) 250, 462
BseNI ACTGG 2 cut(s) 1569, 1937
BseRI GAGGAG 3 cut(s) 67, 1247, 1748
BseSI GKGCMC 2 cut(s) 1679, 2136
BseX3I CGGCCG 1 cut(s) 442
BseXI GCAGC 9 cut(s) 252, 270, 348, 394, 1055, 1135, 1409, 1461, 2254
BseYI CCCAGC 2 cut(s) 1772, 2029
Bsh1236I CGCG 6 cut(s) 266, 268, 335, 340, 447, 2055
Bsh1285I CGRYCG 1 cut(s) 445
BshFI GGCC 4 cut(s) 99, 120, 444, 549
BshNI GGYRCC 1 cut(s) 270
BshTI ACCGGT 1 cut(s) 2200
BsiEI CGRYCG 1 cut(s) 445
BsiHKAI GWGCWC 4 cut(s) 414, 1679, 1740, 2136
BsiHKCI CYCGRG 1 cut(s) 167
BsiSI CCGG 6 cut(s) 96, 550, 722, 762, 827, 2201
BsiWI CGTACG 1 cut(s) 1635
BslFI GGGAC 1 cut(s) 1908
BslI CCNNNNNNNGG 3 cut(s) 254, 497, 549
BsmAI GTCTC 5 cut(s) 139, 576, 851, 1250, 1511
BsmBI CGTCTC 1 cut(s) 1250
BsmFI GGGAC 1 cut(s) 1908
BsmI GAATGC 2 cut(s) 1045, 1570
BsnI GGCC 4 cut(s) 99, 120, 444, 549
Bso31I GGTCTC 1 cut(s) 139
BsoBI CYCGRG 1 cut(s) 167
Bsp1286I GDGCHC 6 cut(s) 414, 1299, 1679, 1740, 1986, 2136
Bsp13I TCCGGA 1 cut(s) 826
Bsp143I GATC 2 cut(s) 429, 1663
Bsp1720I GCTNAGC 1 cut(s) 236
BspANI GGCC 4 cut(s) 99, 120, 444, 549
BspCNI CTCAG 2 cut(s) 249, 463
BspEI TCCGGA 1 cut(s) 826
BspFNI CGCG 6 cut(s) 266, 268, 335, 340, 447, 2055
BspHI TCATGA 2 cut(s) 426, 1219
BspLI GGNNCC 4 cut(s) 173, 272, 1140, 1298
BspMI ACCTGC 1 cut(s) 1703
BspPI GGATC 1 cut(s) 1658
BspT107I GGYRCC 1 cut(s) 270
BspTI CTTAAG 1 cut(s) 1693
BspTNI GGTCTC 1 cut(s) 139
BsrDI GCAATG 3 cut(s) 743, 1554, 2252
BsrFI RCCGGY 1 cut(s) 2200
BsrI ACTGG 2 cut(s) 1569, 1937
BssAI RCCGGY 1 cut(s) 2200
BssECI CCNNGG 5 cut(s) 115, 166, 315, 496, 1359
BssMI GATC 2 cut(s) 429, 1663
BssNI GRCGYC 1 cut(s) 213
BssSI CACGAG 1 cut(s) 707
BssT1I CCWWGG 2 cut(s) 496, 1359
Bst2BI CACGAG 1 cut(s) 707
Bst2UI CCWGG 2 cut(s) 275, 317
Bst4CI ACNGT 6 cut(s) 187, 514, 637, 1404, 1458, 1706
Bst6I CTCTTC 2 cut(s) 579, 1740
BstACI GRCGYC 1 cut(s) 213
BstAFI CTTAAG 1 cut(s) 1693
BstAPI GCANNNNNTGC 1 cut(s) 1565
BstBAI YACGTR 3 cut(s) 254, 840, 1155
BstC8I GCNNGC 8 cut(s) 122, 401, 410, 437, 757, 1020, 1395, 1800
BstDEI CTNAG 4 cut(s) 236, 471, 749, 2060
BstDSI CCRYGG 1 cut(s) 115
BstEII GGTNACC 1 cut(s) 2203
BstENI CCTNNNNNAGG 1 cut(s) 495
BstF5I GGATG 7 cut(s) 18, 162, 915, 1312, 1834, 1982, 2225
BstFNI CGCG 6 cut(s) 266, 268, 335, 340, 447, 2055
BstHHI GCGC 6 cut(s) 268, 340, 470, 761, 973, 2055
BstKTI GATC 2 cut(s) 432, 1666
BstMAI GTCTC 5 cut(s) 139, 576, 851, 1250, 1511
BstMBI GATC 2 cut(s) 429, 1663
BstMCI CGRYCG 1 cut(s) 445
BstNI CCWGG 2 cut(s) 275, 317
BstPI GGTNACC 1 cut(s) 2203
BstSCI CCNGG 3 cut(s) 273, 315, 761
BstSFI CTRYAG 1 cut(s) 2124
BstSLI GKGCMC 2 cut(s) 1679, 2136
BstSNI TACGTA 1 cut(s) 1155
BstUI CGCG 6 cut(s) 266, 268, 335, 340, 447, 2055
BstV1I GCAGC 9 cut(s) 252, 270, 348, 394, 1055, 1135, 1409, 1461, 2254
BstZI CGGCCG 1 cut(s) 442
BsuRI GGCC 4 cut(s) 99, 120, 444, 549
BtgI CCRYGG 1 cut(s) 115
BtgZI GCGATG 1 cut(s) 247
BtsCI GGATG 7 cut(s) 18, 162, 915, 1312, 1834, 1982, 2225
BtsI GCAGTG 1 cut(s) 525
BtsIMutI CAGTG 3 cut(s) 525, 1400, 1711
BveI ACCTGC 1 cut(s) 1703
Cac8I GCNNGC 8 cut(s) 122, 401, 410, 437, 757, 1020, 1395, 1800
CaiI CAGNNNCTG 1 cut(s) 1148
CciI TCATGA 2 cut(s) 426, 1219
CfoI GCGC 6 cut(s) 268, 340, 470, 761, 973, 2055
Cfr10I RCCGGY 1 cut(s) 2200
Cfr13I GGNCC 2 cut(s) 157, 724
CseI GACGC 1 cut(s) 1765
Csp6I GTAC 2 cut(s) 202, 1636
CspAI ACCGGT 1 cut(s) 2200
CviAII CATG 9 cut(s) 427, 1082, 1220, 1301, 1330, 1439, 1487, 1780, 1792
CviQI GTAC 2 cut(s) 202, 1636
DdeI CTNAG 4 cut(s) 236, 471, 749, 2060
DpnI GATC 2 cut(s) 431, 1665
DpnII GATC 2 cut(s) 429, 1663
EaeI YGGCCR 4 cut(s) 97, 118, 442, 547
EagI CGGCCG 1 cut(s) 442
Eam1104I CTCTTC 2 cut(s) 579, 1740
EarI CTCTTC 2 cut(s) 579, 1740
EciI GGCGGA 3 cut(s) 25, 800, 1518
EclXI CGGCCG 1 cut(s) 442
Eco105I TACGTA 1 cut(s) 1155
Eco130I CCWWGG 2 cut(s) 496, 1359
Eco24I GRGCYC 2 cut(s) 1299, 1986
Eco31I GGTCTC 1 cut(s) 139
Eco47I GGWCC 2 cut(s) 157, 724
Eco52I CGGCCG 1 cut(s) 442
Eco57I CTGAAG 2 cut(s) 1743, 1992
Eco72I CACGTG 1 cut(s) 254
Eco88I CYCGRG 1 cut(s) 167
Eco91I GGTNACC 1 cut(s) 2203
EcoNI CCTNNNNNAGG 1 cut(s) 495
EcoO109I RGGNCCY 1 cut(s) 157
EcoO65I GGTNACC 1 cut(s) 2203
EcoRII CCWGG 2 cut(s) 273, 315
EcoT14I CCWWGG 2 cut(s) 496, 1359
EcoT22I ATGCAT 2 cut(s) 1440, 1492
EcoT38I GRGCYC 2 cut(s) 1299, 1986
ErhI CCWWGG 2 cut(s) 496, 1359
Esp3I CGTCTC 1 cut(s) 1250
FaeI CATG 9 cut(s) 430, 1085, 1223, 1304, 1333, 1442, 1490, 1783, 1795
FalI AAGNNNNNCTT 2 cut(s) 441, 473
FaqI GGGAC 1 cut(s) 1908
FatI CATG 9 cut(s) 426, 1081, 1219, 1300, 1329, 1438, 1486, 1779, 1791
FauI CCCGC 2 cut(s) 255, 1018
FauNDI CATATG 1 cut(s) 1387
FbaI TGATCA 1 cut(s) 429
FokI GGATG 7 cut(s) 5, 149, 922, 1319, 1841, 1989, 2232
FriOI GRGCYC 2 cut(s) 1299, 1986
FspBI CTAG 3 cut(s) 492, 654, 1353
FspI TGCGCA 1 cut(s) 972
GlaI GCGC 6 cut(s) 267, 339, 469, 760, 972, 2054
GsaI CCCAGC 2 cut(s) 1776, 2033
HaeIII GGCC 4 cut(s) 99, 120, 444, 549
HapII CCGG 6 cut(s) 96, 550, 722, 762, 827, 2201
HgaI GACGC 1 cut(s) 1765
HhaI GCGC 6 cut(s) 268, 340, 470, 761, 973, 2055
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 9 cut(s) 430, 1085, 1223, 1304, 1333, 1442, 1490, 1783, 1795
Hin6I GCGC 6 cut(s) 266, 338, 468, 759, 971, 2053
HinP1I GCGC 6 cut(s) 266, 338, 468, 759, 971, 2053
HindIII AAGCTT 3 cut(s) 878, 1593, 1755
HinfI GANTC 3 cut(s) 87, 914, 1925
HpaII CCGG 6 cut(s) 96, 550, 722, 762, 827, 2201
HphI GGTGA 6 cut(s) 175, 788, 1193, 1219, 1556, 2215
Hpy166II GTNNAC 4 cut(s) 455, 1677, 1702, 2134
Hpy188I TCNGA 5 cut(s) 86, 895, 1972, 1988, 2037
Hpy8I GTNNAC 4 cut(s) 455, 1677, 1702, 2134
Hpy99I CGWCG 5 cut(s) 191, 215, 218, 485, 946
HpyCH4III ACNGT 6 cut(s) 187, 514, 637, 1404, 1458, 1706
HpyCH4IV ACGT 8 cut(s) 213, 253, 839, 941, 1154, 1259, 1634, 1638
HpyF3I CTNAG 4 cut(s) 236, 471, 749, 2060
HpySE526I ACGT 8 cut(s) 213, 253, 839, 941, 1154, 1259, 1634, 1638
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 9 cut(s) 430, 1085, 1223, 1304, 1333, 1442, 1490, 1783, 1795
HspAI GCGC 6 cut(s) 266, 338, 468, 759, 971, 2053
Kpn2I TCCGGA 1 cut(s) 826
Ksp22I TGATCA 1 cut(s) 429
Kzo9I GATC 2 cut(s) 429, 1663
LmnI GCTCC 4 cut(s) 1105, 1138, 1302, 1735
Lsp1109I GCAGC 9 cut(s) 252, 270, 348, 394, 1055, 1135, 1409, 1461, 2254
LweI GCATC 7 cut(s) 270, 1005, 1135, 1297, 1785, 1822, 1845
MaeI CTAG 3 cut(s) 492, 654, 1353
MaeII ACGT 8 cut(s) 213, 253, 839, 941, 1154, 1259, 1634, 1638
MaeIII GTNAC 5 cut(s) 181, 730, 1207, 2203, 2249
MalI GATC 2 cut(s) 431, 1665
MboI GATC 2 cut(s) 429, 1663
MboII GAAGA 6 cut(s) 274, 566, 607, 1631, 1668, 1757
MhlI GDGCHC 6 cut(s) 414, 1299, 1679, 1740, 1986, 2136
MluCI AATT 5 cut(s) 578, 869, 925, 1066, 2193
MlyI GAGTC 1 cut(s) 923
MmeI TCCRAC 2 cut(s) 645, 939
Mph1103I ATGCAT 2 cut(s) 1440, 1492
MroI TCCGGA 1 cut(s) 826
MroXI GAANNNNTTC 1 cut(s) 308
MseI TTAA 7 cut(s) 537, 642, 903, 987, 1694, 1722, 1752
MslI CAYNNNNRTG 6 cut(s) 425, 1172, 1305, 1332, 2023, 2139
MspA1I CMGCKG 2 cut(s) 124, 385
MspCI CTTAAG 1 cut(s) 1693
MspI CCGG 6 cut(s) 96, 550, 722, 762, 827, 2201
MspR9I CCNGG 3 cut(s) 275, 317, 763
Mva1269I GAATGC 2 cut(s) 1045, 1570
MvaI CCWGG 2 cut(s) 275, 317
MvnI CGCG 6 cut(s) 266, 268, 335, 340, 447, 2055
NciI CCSGG 1 cut(s) 763
NdeI CATATG 1 cut(s) 1387
NdeII GATC 2 cut(s) 429, 1663
NlaIII CATG 9 cut(s) 430, 1085, 1223, 1304, 1333, 1442, 1490, 1783, 1795
NlaIV GGNNCC 4 cut(s) 173, 272, 1140, 1298
NmeAIII GCCGAG 2 cut(s) 420, 429
NmuCI GTSAC 4 cut(s) 181, 1207, 2203, 2249
NsbI TGCGCA 1 cut(s) 972
NsiI ATGCAT 2 cut(s) 1440, 1492
OliI CACNNNNGTG 1 cut(s) 2023
PagI TCATGA 2 cut(s) 426, 1219
PctI GAATGC 2 cut(s) 1045, 1570
PdmI GAANNNNTTC 1 cut(s) 308
PfeI GAWTC 2 cut(s) 87, 1925
Pfl23II CGTACG 1 cut(s) 1635
PinAI ACCGGT 1 cut(s) 2200
PleI GAGTC 1 cut(s) 922
PmaCI CACGTG 1 cut(s) 254
PmlI CACGTG 1 cut(s) 254
PpsI GAGTC 1 cut(s) 922
Ppu21I YACGTR 3 cut(s) 254, 840, 1155
PpuMI RGGWCCY 1 cut(s) 157
PshBI ATTAAT 1 cut(s) 537
PsiI TTATAA 2 cut(s) 630, 738
Psp5II RGGWCCY 1 cut(s) 157
Psp6I CCWGG 2 cut(s) 273, 315
PspCI CACGTG 1 cut(s) 254
PspEI GGTNACC 1 cut(s) 2203
PspFI CCCAGC 2 cut(s) 1772, 2029
PspGI CCWGG 2 cut(s) 273, 315
PspLI CGTACG 1 cut(s) 1635
PspN4I GGNNCC 4 cut(s) 173, 272, 1140, 1298
PspPI GGNCC 2 cut(s) 157, 724
PspPPI RGGWCCY 1 cut(s) 157
PstNI CAGNNNCTG 1 cut(s) 1148
RsaI GTAC 2 cut(s) 203, 1637
RsaNI GTAC 2 cut(s) 202, 1636
RseI CAYNNNNRTG 6 cut(s) 425, 1172, 1305, 1332, 2023, 2139
SaqAI TTAA 7 cut(s) 537, 642, 903, 987, 1694, 1722, 1752
Sau3AI GATC 2 cut(s) 429, 1663
Sau96I GGNCC 2 cut(s) 157, 724
SchI GAGTC 1 cut(s) 923
ScrFI CCNGG 3 cut(s) 275, 317, 763
SduI GDGCHC 6 cut(s) 414, 1299, 1679, 1740, 1986, 2136
SfaNI GCATC 7 cut(s) 270, 1005, 1135, 1297, 1785, 1822, 1845
SfcI CTRYAG 1 cut(s) 2124
SinI GGWCC 2 cut(s) 157, 724
SmiMI CAYNNNNRTG 6 cut(s) 425, 1172, 1305, 1332, 2023, 2139
SmlI CTYRAG 4 cut(s) 47, 881, 1115, 1693
SmoI CTYRAG 4 cut(s) 47, 881, 1115, 1693
SnaBI TACGTA 1 cut(s) 1155
Sse9I AATT 5 cut(s) 578, 869, 925, 1066, 2193
SspI AATATT 3 cut(s) 1162, 1240, 1879
SspMI CTAG 3 cut(s) 492, 654, 1353
StyD4I CCNGG 3 cut(s) 273, 315, 761
StyI CCWWGG 2 cut(s) 496, 1359
TaaI ACNGT 6 cut(s) 187, 514, 637, 1404, 1458, 1706
TaiI ACGT 8 cut(s) 216, 256, 842, 944, 1157, 1262, 1637, 1641
TaqI TCGA 5 cut(s) 189, 216, 480, 983, 1535
TaqII GACCGA 1 cut(s) 121
TasI AATT 5 cut(s) 578, 869, 925, 1066, 2193
TatI WGTACW 1 cut(s) 201
TauI GCSGC 5 cut(s) 338, 367, 388, 447, 1025
TfiI GAWTC 2 cut(s) 87, 1925
Tru1I TTAA 7 cut(s) 537, 642, 903, 987, 1694, 1722, 1752
Tru9I TTAA 7 cut(s) 537, 642, 903, 987, 1694, 1722, 1752
TscAI CASTG 3 cut(s) 532, 1407, 1711
TseFI GTSAC 4 cut(s) 181, 1207, 2203, 2249
TseI GCWGC 9 cut(s) 240, 258, 361, 382, 1043, 1148, 1397, 1449, 2242
Tsp45I GTSAC 4 cut(s) 181, 1207, 2203, 2249
TspGWI ACGGA 4 cut(s) 127, 169, 1151, 2090
TspRI CASTG 3 cut(s) 532, 1407, 1711
Vha464I CTTAAG 1 cut(s) 1693
VneI GTGCAC 2 cut(s) 1675, 2132
VpaK11BI GGWCC 2 cut(s) 157, 724
VspI ATTAAT 1 cut(s) 537
XagI CCTNNNNNAGG 1 cut(s) 495
XapI RAATTY 2 cut(s) 925, 1066
XmnI GAANNNNTTC 1 cut(s) 308
XspI CTAG 3 cut(s) 492, 654, 1353
ZraI GACGTC 1 cut(s) 214
Zsp2I ATGCAT 2 cut(s) 1440, 1492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.