FvH4_5g17860

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008137
Physical Location & Seq
Reverse (-)
10246222 .. 10247097
876 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g17860.t1

Sequence Viewer

Length: 552 bp
ATGGCGTCTAAAGCTATTCCTTGCGCAGTTCTGGTATTCAGGATATTCACCCTTGCAGCCTGCGTTGCTTCTGGACTGTTACTTGCTCTCGACAACTTTACAACTGCTAATGGAAATCAAAAACTACGTTTTCAGGATGTGGTCACATTCAGGTATGTGTTTTCTGCAACAGCAATTGGAGCAGCATATAACATCCTACAGCTACCATTTAACATATACTACGCTTCCACCCAAAAGCGTTTGATACGCAACGGATGCATGCCGGAGTTTGATTTCTATGCCGACAAGGTAGTGAGCTCGATTCTCTGTTCAGCTGTGGGAGCTGGTTTTGGAGCGGGATTTGAGCTTAAAAAGGTGATCAAAGTTTTGATTCCTCTGCTGGCATCCCTAGGCGTCCTCGACAACTTGCAGGAATCACAAACCAAGAACGATGGCTTCTATGAGAAGGCTATTATCGCTACCGGAATTCTCTTGGTTGGAGCTGTTTGCATGGCTGTGGTTTCAATCCTTACTTCCATCACTCGAACTTCAAATCCCGGTTTCTTCCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

184

Amino Acids

19.71

Weight (kDa)

9.18

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 6 - 113 8.9e-16 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 25
AccBSI CCGCTC 1 cut(s) 335
AciI CCGC 1 cut(s) 335
AcsI RAATTY 1 cut(s) 465
AcyI GRCGYC 2 cut(s) 5, 393
AgsI TTSAA 2 cut(s) 504, 531
AloI GAACNNNNNNTCC 2 cut(s) 517, 549
AluBI AGCT 7 cut(s) 14, 202, 297, 314, 323, 346, 482
AluI AGCT 7 cut(s) 14, 202, 297, 314, 323, 346, 482
Alw21I GWGCWC 1 cut(s) 299
ApeKI GCWGC 2 cut(s) 56, 182
ApoI RAATTY 1 cut(s) 465
AspA2I CCTAGG 1 cut(s) 388
AspLEI GCGC 1 cut(s) 26
AsuC2I CCSGG 1 cut(s) 537
AsuHPI GGTGA 2 cut(s) 40, 367
AvrII CCTAGG 1 cut(s) 388
BanII GRGCYC 1 cut(s) 299
Bbv12I GWGCWC 1 cut(s) 299
BbvI GCAGC 2 cut(s) 68, 194
BccI CCATC 2 cut(s) 425, 524
BclI TGATCA 1 cut(s) 357
BcnI CCSGG 1 cut(s) 537
BfaI CTAG 1 cut(s) 389
BfmI CTRYAG 1 cut(s) 197
BisI GCNGC 2 cut(s) 57, 183
BlnI CCTAGG 1 cut(s) 388
BlsI GCNGC 2 cut(s) 58, 184
Bme1390I CCNGG 1 cut(s) 537
BmrFI CCNGG 1 cut(s) 537
BmsI GCATC 2 cut(s) 245, 392
BpuMI CCSGG 1 cut(s) 537
BsaHI GRCGYC 2 cut(s) 5, 393
BsaJI CCNNGG 1 cut(s) 388
BsaWI WCCGGW 2 cut(s) 461, 546
BseDI CCNNGG 1 cut(s) 388
BseGI GGATG 4 cut(s) 142, 192, 260, 383
BseXI GCAGC 2 cut(s) 68, 194
BsiHKAI GWGCWC 1 cut(s) 299
BsiSI CCGG 4 cut(s) 263, 462, 537, 547
Bsp1286I GDGCHC 1 cut(s) 299
Bsp143I GATC 1 cut(s) 357
BspACI CCGC 1 cut(s) 335
BsrBI CCGCTC 1 cut(s) 335
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 1 cut(s) 357
BssNI GRCGYC 2 cut(s) 5, 393
BssT1I CCWWGG 1 cut(s) 388
Bst4CI ACNGT 1 cut(s) 78
BstACI GRCGYC 2 cut(s) 5, 393
BstAPI GCANNNNNTGC 1 cut(s) 255
BstC8I GCNNGC 3 cut(s) 61, 260, 381
BstF5I GGATG 4 cut(s) 142, 192, 260, 383
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 1 cut(s) 360
BstMBI GATC 1 cut(s) 357
BstMWI GCNNNNNNNGC 6 cut(s) 11, 65, 179, 255, 320, 455
BstNSI RCATGY 1 cut(s) 262
BstSCI CCNGG 1 cut(s) 535
BstSFI CTRYAG 1 cut(s) 197
BstV1I GCAGC 2 cut(s) 68, 194
BtsCI GGATG 4 cut(s) 142, 192, 260, 383
Cac8I GCNNGC 3 cut(s) 61, 260, 381
CfoI GCGC 1 cut(s) 26
CseI GACGC 1 cut(s) 382
CviAII CATG 2 cut(s) 259, 490
DpnI GATC 1 cut(s) 359
DpnII GATC 1 cut(s) 357
Ecl136II GAGCTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 388
Eco24I GRGCYC 1 cut(s) 299
Eco53kI GAGCTC 1 cut(s) 297
EcoICRI GAGCTC 1 cut(s) 297
EcoRI GAATTC 1 cut(s) 465
EcoT14I CCWWGG 1 cut(s) 388
EcoT22I ATGCAT 1 cut(s) 260
EcoT38I GRGCYC 1 cut(s) 299
ErhI CCWWGG 1 cut(s) 388
FaeI CATG 2 cut(s) 262, 493
FaiI YATR 9 cut(s) 156, 187, 189, 215, 217, 260, 279, 441, 491
FatI CATG 2 cut(s) 258, 489
FauI CCCGC 1 cut(s) 328
FbaI TGATCA 1 cut(s) 357
Fnu4HI GCNGC 2 cut(s) 57, 183
FokI GGATG 4 cut(s) 149, 179, 267, 370
FriOI GRGCYC 1 cut(s) 299
Fsp4HI GCNGC 2 cut(s) 57, 183
FspBI CTAG 1 cut(s) 389
FspI TGCGCA 1 cut(s) 25
GlaI GCGC 1 cut(s) 25
GluI GCNGC 2 cut(s) 57, 183
HapII CCGG 4 cut(s) 263, 462, 537, 547
HgaI GACGC 1 cut(s) 382
HhaI GCGC 1 cut(s) 26
Hin1I GRCGYC 2 cut(s) 5, 393
Hin1II CATG 2 cut(s) 262, 493
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HinfI GANTC 3 cut(s) 301, 370, 413
HpaII CCGG 4 cut(s) 263, 462, 537, 547
HphI GGTGA 2 cut(s) 40, 367
Hpy188III TCNNGA 4 cut(s) 40, 72, 89, 134
HpyAV CCTTC 1 cut(s) 439
HpyCH4III ACNGT 1 cut(s) 78
HpyCH4IV ACGT 1 cut(s) 127
HpyCH4V TGCA 5 cut(s) 56, 167, 258, 409, 489
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 65, 179, 255, 320, 455
HpySE526I ACGT 1 cut(s) 127
Hsp92I GRCGYC 2 cut(s) 5, 393
Hsp92II CATG 2 cut(s) 262, 493
HspAI GCGC 1 cut(s) 24
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 1 cut(s) 357
LmnI GCTCC 4 cut(s) 179, 320, 332, 479
Lsp1109I GCAGC 2 cut(s) 68, 194
LweI GCATC 2 cut(s) 245, 392
MaeI CTAG 1 cut(s) 389
MaeII ACGT 1 cut(s) 127
MaeIII GTNAC 2 cut(s) 78, 142
MalI GATC 1 cut(s) 359
MbiI CCGCTC 1 cut(s) 335
MboI GATC 1 cut(s) 357
MboII GAAGA 1 cut(s) 535
MfeI CAATTG 1 cut(s) 174
MhlI GDGCHC 1 cut(s) 299
MluCI AATT 2 cut(s) 174, 465
MmeI TCCRAC 1 cut(s) 457
MnlI CCTC 2 cut(s) 384, 407
Mph1103I ATGCAT 1 cut(s) 260
MseI TTAA 2 cut(s) 210, 348
MslI CAYNNNNRTG 1 cut(s) 494
MspA1I CMGCKG 1 cut(s) 314
MspI CCGG 4 cut(s) 263, 462, 537, 547
MspR9I CCNGG 1 cut(s) 537
MunI CAATTG 1 cut(s) 174
MwoI GCNNNNNNNGC 6 cut(s) 11, 65, 179, 255, 320, 455
NciI CCSGG 1 cut(s) 537
NdeII GATC 1 cut(s) 357
NlaIII CATG 2 cut(s) 262, 493
NmuCI GTSAC 1 cut(s) 142
NsbI TGCGCA 1 cut(s) 25
NsiI ATGCAT 1 cut(s) 260
NspI RCATGY 1 cut(s) 262
PaeI GCATGC 1 cut(s) 262
PfeI GAWTC 3 cut(s) 301, 370, 413
PkrI GCNGC 2 cut(s) 58, 184
Psp124BI GAGCTC 1 cut(s) 299
PvuII CAGCTG 1 cut(s) 314
RseI CAYNNNNRTG 1 cut(s) 494
SacI GAGCTC 1 cut(s) 299
SaqAI TTAA 2 cut(s) 210, 348
SatI GCNGC 2 cut(s) 57, 183
Sau3AI GATC 1 cut(s) 357
ScrFI CCNGG 1 cut(s) 537
SduI GDGCHC 1 cut(s) 299
SfaNI GCATC 2 cut(s) 245, 392
SfcI CTRYAG 1 cut(s) 197
SmiMI CAYNNNNRTG 1 cut(s) 494
SphI GCATGC 1 cut(s) 262
Sse9I AATT 2 cut(s) 174, 465
SsiI CCGC 1 cut(s) 335
SspMI CTAG 1 cut(s) 389
SstI GAGCTC 1 cut(s) 299
StyD4I CCNGG 1 cut(s) 535
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 1 cut(s) 78
TaiI ACGT 1 cut(s) 130
TaqI TCGA 4 cut(s) 90, 299, 399, 523
TasI AATT 2 cut(s) 174, 465
TfiI GAWTC 3 cut(s) 301, 370, 413
Tru1I TTAA 2 cut(s) 210, 348
Tru9I TTAA 2 cut(s) 210, 348
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 2 cut(s) 56, 182
Tsp45I GTSAC 1 cut(s) 142
TspGWI ACGGA 1 cut(s) 267
XapI RAATTY 1 cut(s) 465
XceI RCATGY 1 cut(s) 262
XmaJI CCTAGG 1 cut(s) 388
XspI CTAG 1 cut(s) 389
Zsp2I ATGCAT 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.