Rh7AG008300

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0008137
Physical Location & Seq
Forward (+)
556504 .. 557169
666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG008300.1

Sequence Viewer

Length: 543 bp
ATGTCTAAAGCTATTCCTGCCCTAGTTCTTGTATTCAGGATCCTTACCCTTTCAGCCTGTGTTGCTTCTGGGCTGCTTCTCGTTCTCGACAACTTTAAACATGAGGATGGTGATAAACTACATTTTTATGACATCATTACATTCAGGCATGTGCTTTCTGCAGCAGCAATTGGAGCAGCATACAGCCTACTACAGCTACCATTTAACATATACTATGCTTGCACCAATAAGCGCTTGATACGCAACAGGTGCATGCCGGAGTTTGATTTCTATTCCGACAGGGTAGTGACCTTCGTTCTAGCTTCGGGTGTGGGAGCTGGTTTTGCAGCTGGATTTGAGCTCAAAAAGTTTTTCAAACTATTACTTCAAGTGGCGTCACTAGGAATTGACTACAACAACTTCGATCAAGAACTAGCCAAGTACAATGACTTCTTTGAGAAGGCAATTATTTCCAGCGGAATTCTCTTGGTTGGAGCTGTTTGCATGGCTGTGGTTTCGATTCTCACTTCCATCACTCGGACTTCAAATTCGGGTTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

180

Amino Acids

19.88

Weight (kDa)

8.34

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 6 - 111 9.3e-14 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 456
AclWI GGATC 2 cut(s) 34, 47
AcsI RAATTY 2 cut(s) 459, 526
AcyI GRCGYC 1 cut(s) 374
AfaI GTAC 1 cut(s) 422
AfeI AGCGCT 1 cut(s) 233
AfiI CCNNNNNNNGG 1 cut(s) 516
AgsI TTSAA 3 cut(s) 355, 368, 525
AluBI AGCT 7 cut(s) 11, 196, 302, 317, 329, 340, 476
AluI AGCT 7 cut(s) 11, 196, 302, 317, 329, 340, 476
Alw21I GWGCWC 1 cut(s) 342
AlwI GGATC 2 cut(s) 34, 47
Aor51HI AGCGCT 1 cut(s) 233
ApeKI GCWGC 5 cut(s) 73, 161, 164, 176, 326
ApoI RAATTY 2 cut(s) 459, 526
ArsI GACNNNNNNTTYG 2 cut(s) 511, 543
AspLEI GCGC 1 cut(s) 234
AsuHPI GGTGA 1 cut(s) 122
BamHI GGATCC 1 cut(s) 39
BanII GRGCYC 1 cut(s) 342
BarI GAAGNNNNNNTAC 2 cut(s) 413, 445
Bbv12I GWGCWC 1 cut(s) 342
BbvI GCAGC 5 cut(s) 60, 173, 176, 188, 338
BccI CCATC 2 cut(s) 101, 518
BfaI CTAG 4 cut(s) 23, 299, 380, 413
BfmI CTRYAG 2 cut(s) 159, 191
BfoI RGCGCY 1 cut(s) 235
BisI GCNGC 5 cut(s) 74, 162, 165, 177, 327
BlsI GCNGC 5 cut(s) 75, 163, 166, 178, 328
BmiI GGNNCC 1 cut(s) 41
BsaHI GRCGYC 1 cut(s) 374
Bsc4I CCNNNNNNNGG 1 cut(s) 516
BseGI GGATG 1 cut(s) 112
BseLI CCNNNNNNNGG 1 cut(s) 516
BseXI GCAGC 5 cut(s) 60, 173, 176, 188, 338
BsiHKAI GWGCWC 1 cut(s) 342
BsiSI CCGG 1 cut(s) 257
BslI CCNNNNNNNGG 1 cut(s) 516
Bsp1286I GDGCHC 1 cut(s) 342
Bsp143I GATC 2 cut(s) 39, 403
BspACI CCGC 1 cut(s) 456
BspLI GGNNCC 1 cut(s) 41
BspMAI CTGCAG 1 cut(s) 163
BspPI GGATC 2 cut(s) 34, 47
BssMI GATC 2 cut(s) 39, 403
BssNI GRCGYC 1 cut(s) 374
BstACI GRCGYC 1 cut(s) 374
BstAPI GCANNNNNTGC 1 cut(s) 249
BstC8I GCNNGC 2 cut(s) 220, 254
BstF5I GGATG 1 cut(s) 112
BstH2I RGCGCY 1 cut(s) 235
BstHHI GCGC 1 cut(s) 234
BstKTI GATC 2 cut(s) 42, 406
BstMBI GATC 2 cut(s) 39, 403
BstMWI GCNNNNNNNGC 6 cut(s) 17, 62, 173, 240, 249, 323
BstNSI RCATGY 2 cut(s) 152, 256
BstSFI CTRYAG 2 cut(s) 159, 191
BstV1I GCAGC 5 cut(s) 60, 173, 176, 188, 338
BstX2I RGATCY 1 cut(s) 39
BstYI RGATCY 1 cut(s) 39
BtsCI GGATG 1 cut(s) 112
Cac8I GCNNGC 2 cut(s) 220, 254
CfoI GCGC 1 cut(s) 234
CseI GACGC 1 cut(s) 363
Csp6I GTAC 1 cut(s) 421
CviAII CATG 4 cut(s) 101, 149, 253, 484
CviQI GTAC 1 cut(s) 421
DpnI GATC 2 cut(s) 41, 405
DpnII GATC 2 cut(s) 39, 403
DraI TTTAAA 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 340
Eco24I GRGCYC 1 cut(s) 342
Eco47III AGCGCT 1 cut(s) 233
Eco53kI GAGCTC 1 cut(s) 340
EcoICRI GAGCTC 1 cut(s) 340
EcoRI GAATTC 1 cut(s) 459
EcoT38I GRGCYC 1 cut(s) 342
FaeI CATG 4 cut(s) 104, 152, 256, 487
FaiI YATR 9 cut(s) 102, 129, 150, 181, 209, 211, 216, 254, 485
FatI CATG 4 cut(s) 100, 148, 252, 483
Fnu4HI GCNGC 5 cut(s) 74, 162, 165, 177, 327
FokI GGATG 1 cut(s) 119
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 5 cut(s) 74, 162, 165, 177, 327
FspBI CTAG 4 cut(s) 23, 299, 380, 413
GlaI GCGC 1 cut(s) 233
GluI GCNGC 5 cut(s) 74, 162, 165, 177, 327
HaeII RGCGCY 1 cut(s) 235
HapII CCGG 1 cut(s) 257
HgaI GACGC 1 cut(s) 363
HhaI GCGC 1 cut(s) 234
Hin1I GRCGYC 1 cut(s) 374
Hin1II CATG 4 cut(s) 104, 152, 256, 487
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HinfI GANTC 1 cut(s) 499
HpaII CCGG 1 cut(s) 257
HphI GGTGA 1 cut(s) 122
Hpy188I TCNGA 3 cut(s) 277, 519, 542
Hpy188III TCNNGA 3 cut(s) 37, 86, 407
HpyAV CCTTC 2 cut(s) 301, 433
HpyCH4V TGCA 5 cut(s) 161, 222, 252, 326, 483
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 62, 173, 240, 249, 323
Hsp92I GRCGYC 1 cut(s) 374
Hsp92II CATG 4 cut(s) 104, 152, 256, 487
HspAI GCGC 1 cut(s) 232
Kzo9I GATC 2 cut(s) 39, 403
LmnI GCTCC 3 cut(s) 173, 314, 473
Lsp1109I GCAGC 5 cut(s) 60, 173, 176, 188, 338
MaeI CTAG 4 cut(s) 23, 299, 380, 413
MaeIII GTNAC 2 cut(s) 286, 375
MalI GATC 2 cut(s) 41, 405
MboI GATC 2 cut(s) 39, 403
MboII GAAGA 1 cut(s) 529
MfeI CAATTG 1 cut(s) 168
MflI RGATCY 1 cut(s) 39
MhlI GDGCHC 1 cut(s) 342
MluCI AATT 5 cut(s) 168, 384, 444, 459, 526
MmeI TCCRAC 2 cut(s) 300, 451
MnlI CCTC 1 cut(s) 97
MseI TTAA 2 cut(s) 96, 204
MslI CAYNNNNRTG 3 cut(s) 105, 126, 488
MspA1I CMGCKG 2 cut(s) 329, 456
MspI CCGG 1 cut(s) 257
MunI CAATTG 1 cut(s) 168
MwoI GCNNNNNNNGC 6 cut(s) 17, 62, 173, 240, 249, 323
NdeII GATC 2 cut(s) 39, 403
NlaIII CATG 4 cut(s) 104, 152, 256, 487
NlaIV GGNNCC 1 cut(s) 41
NmuCI GTSAC 2 cut(s) 286, 375
NspI RCATGY 2 cut(s) 152, 256
PaeI GCATGC 1 cut(s) 256
PfeI GAWTC 1 cut(s) 499
PkrI GCNGC 5 cut(s) 75, 163, 166, 178, 328
Psp124BI GAGCTC 1 cut(s) 342
PspN4I GGNNCC 1 cut(s) 41
PstI CTGCAG 1 cut(s) 163
PsuI RGATCY 1 cut(s) 39
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 1 cut(s) 422
RsaNI GTAC 1 cut(s) 421
RseI CAYNNNNRTG 3 cut(s) 105, 126, 488
SacI GAGCTC 1 cut(s) 342
SaqAI TTAA 2 cut(s) 96, 204
SatI GCNGC 5 cut(s) 74, 162, 165, 177, 327
Sau3AI GATC 2 cut(s) 39, 403
SduI GDGCHC 1 cut(s) 342
SetI ASST 9 cut(s) 13, 198, 251, 293, 304, 319, 331, 342, 478
SfcI CTRYAG 2 cut(s) 159, 191
SmiMI CAYNNNNRTG 3 cut(s) 105, 126, 488
SphI GCATGC 1 cut(s) 256
Sse9I AATT 5 cut(s) 168, 384, 444, 459, 526
SsiI CCGC 1 cut(s) 456
SspMI CTAG 4 cut(s) 23, 299, 380, 413
SstI GAGCTC 1 cut(s) 342
TaqI TCGA 3 cut(s) 87, 402, 497
TasI AATT 5 cut(s) 168, 384, 444, 459, 526
TatI WGTACW 1 cut(s) 420
TfiI GAWTC 1 cut(s) 499
Tru1I TTAA 2 cut(s) 96, 204
Tru9I TTAA 2 cut(s) 96, 204
TseFI GTSAC 2 cut(s) 286, 375
TseI GCWGC 5 cut(s) 73, 161, 164, 176, 326
Tsp45I GTSAC 2 cut(s) 286, 375
XapI RAATTY 2 cut(s) 459, 526
XceI RCATGY 2 cut(s) 152, 256
XspI CTAG 4 cut(s) 23, 299, 380, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.