RLG00000005510

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0008137
Physical Location & Seq
Reverse (-)
68193058 .. 68193716
659 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005510

Sequence Viewer

Length: 534 bp
ATGGCATCTGAAGCTATTCCTTGCGCAGTTCTGGTATTCAGGATGTTTACCCTTGTGGCCTGCGTTGCTTCTGGCTGGCTACTTGTTCTCGACCACTTTACAATTTCTAATCAGAAATTACGTTTTCAGGATCTGGTTACGTTCAGGTATGTGCTTTCTTCAGCAGTAATTGGAGCAGCATACAGCATACTACAGCTACCTTTTAATATATACTATGCTTCCACCGAAAAGCGTTTGATACGCAACGGATGCATGCCGGAGTTTGATTTCTATGCCGACAAGGTAGTGAGCTTGATTCTATCTTCGGCTGTGGGAGCTGGTTTTGGAGCGGGAGTTGAGCTCAAAAAGTTTATCGAAGCGTTACTTCCTCTGCTTGGCCTTGACAACTTGAGGGAAGCACAAACCAAGAACGATGACTTCTATGAGAAGGCTCTTGTCGCTACCGGAATTCTCTTGGTTGGAGCTGTTTGCATGGCTGTGGTTTCAATCCTCACTTCCATTGCTCGAACTCCCGGTTTCTTCGGGTACCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

178

Amino Acids

19.36

Weight (kDa)

7.62

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 8 - 112 3.6e-16 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 25
Acc65I GGTACC 1 cut(s) 525
AccB1I GGYRCC 1 cut(s) 525
AccBSI CCGCTC 1 cut(s) 329
AciI CCGC 1 cut(s) 329
AclWI GGATC 1 cut(s) 138
AcsI RAATTY 1 cut(s) 447
AcuI CTGAAG 2 cut(s) 30, 144
AfaI GTAC 1 cut(s) 527
AfiI CCNNNNNNNGG 1 cut(s) 374
AgeI ACCGGT 1 cut(s) 528
AgsI TTSAA 1 cut(s) 486
AluBI AGCT 6 cut(s) 14, 196, 291, 317, 340, 464
AluI AGCT 6 cut(s) 14, 196, 291, 317, 340, 464
Alw21I GWGCWC 1 cut(s) 342
AlwI GGATC 1 cut(s) 138
AlwNI CAGNNNCTG 1 cut(s) 133
AoxI GGCC 2 cut(s) 57, 376
ApeKI GCWGC 1 cut(s) 176
ApoI RAATTY 1 cut(s) 447
AsiGI ACCGGT 1 cut(s) 528
Asp700I GAANNNNTTC 1 cut(s) 15
Asp718I GGTACC 1 cut(s) 525
AspLEI GCGC 1 cut(s) 26
AsuC2I CCSGG 1 cut(s) 513
BanI GGYRCC 1 cut(s) 525
BanII GRGCYC 1 cut(s) 342
Bbv12I GWGCWC 1 cut(s) 342
BbvI GCAGC 1 cut(s) 188
BcnI CCSGG 1 cut(s) 513
BfmI CTRYAG 1 cut(s) 191
BisI GCNGC 1 cut(s) 177
BlsI GCNGC 1 cut(s) 178
Bme1390I CCNGG 1 cut(s) 513
BmiI GGNNCC 1 cut(s) 527
BmrFI CCNGG 1 cut(s) 513
BmsI GCATC 2 cut(s) 14, 239
BplI GAGNNNNNCTC 2 cut(s) 324, 356
BpuEI CTTGAG 1 cut(s) 409
BpuMI CCSGG 1 cut(s) 513
BsaWI WCCGGW 2 cut(s) 443, 528
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bsc4I CCNNNNNNNGG 1 cut(s) 374
Bse118I RCCGGY 1 cut(s) 528
Bse3DI GCAATG 1 cut(s) 498
BseGI GGATG 2 cut(s) 48, 254
BseLI CCNNNNNNNGG 1 cut(s) 374
BseMI GCAATG 1 cut(s) 498
BseXI GCAGC 1 cut(s) 188
BshFI GGCC 2 cut(s) 59, 378
BshNI GGYRCC 1 cut(s) 525
BshTI ACCGGT 1 cut(s) 528
BsiHKAI GWGCWC 1 cut(s) 342
BsiSI CCGG 4 cut(s) 257, 444, 513, 529
BslI CCNNNNNNNGG 1 cut(s) 374
BsnI GGCC 2 cut(s) 59, 378
Bsp1286I GDGCHC 1 cut(s) 342
Bsp143I GATC 1 cut(s) 130
BspACI CCGC 1 cut(s) 329
BspANI GGCC 2 cut(s) 59, 378
BspLI GGNNCC 1 cut(s) 527
BspPI GGATC 1 cut(s) 138
BspT107I GGYRCC 1 cut(s) 525
BsrBI CCGCTC 1 cut(s) 329
BsrDI GCAATG 1 cut(s) 498
BsrFI RCCGGY 1 cut(s) 528
BssAI RCCGGY 1 cut(s) 528
BssMI GATC 1 cut(s) 130
BstAPI GCANNNNNTGC 1 cut(s) 249
BstC8I GCNNGC 3 cut(s) 61, 77, 254
BstF5I GGATG 2 cut(s) 48, 254
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 1 cut(s) 133
BstMBI GATC 1 cut(s) 130
BstMWI GCNNNNNNNGC 5 cut(s) 11, 65, 249, 314, 437
BstNSI RCATGY 1 cut(s) 256
BstSCI CCNGG 1 cut(s) 511
BstSFI CTRYAG 1 cut(s) 191
BstV1I GCAGC 1 cut(s) 188
BstX2I RGATCY 1 cut(s) 130
BstYI RGATCY 1 cut(s) 130
BsuRI GGCC 2 cut(s) 59, 378
BtsCI GGATG 2 cut(s) 48, 254
Cac8I GCNNGC 3 cut(s) 61, 77, 254
CaiI CAGNNNCTG 1 cut(s) 133
CfoI GCGC 1 cut(s) 26
Cfr10I RCCGGY 1 cut(s) 528
Csp6I GTAC 1 cut(s) 526
CspAI ACCGGT 1 cut(s) 528
CviAII CATG 2 cut(s) 253, 472
CviQI GTAC 1 cut(s) 526
DpnI GATC 1 cut(s) 132
DpnII GATC 1 cut(s) 130
Ecl136II GAGCTC 1 cut(s) 340
Eco24I GRGCYC 1 cut(s) 342
Eco53kI GAGCTC 1 cut(s) 340
Eco57I CTGAAG 2 cut(s) 30, 144
EcoICRI GAGCTC 1 cut(s) 340
EcoRI GAATTC 1 cut(s) 447
EcoT22I ATGCAT 1 cut(s) 254
EcoT38I GRGCYC 1 cut(s) 342
FaeI CATG 2 cut(s) 256, 475
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FatI CATG 2 cut(s) 252, 471
FauI CCCGC 1 cut(s) 322
Fnu4HI GCNGC 1 cut(s) 177
FokI GGATG 2 cut(s) 55, 261
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 1 cut(s) 177
FspI TGCGCA 1 cut(s) 25
GlaI GCGC 1 cut(s) 25
GluI GCNGC 1 cut(s) 177
HaeIII GGCC 2 cut(s) 59, 378
HapII CCGG 4 cut(s) 257, 444, 513, 529
HhaI GCGC 1 cut(s) 26
Hin1II CATG 2 cut(s) 256, 475
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HinfI GANTC 1 cut(s) 295
HpaII CCGG 4 cut(s) 257, 444, 513, 529
Hpy166II GTNNAC 1 cut(s) 48
Hpy188I TCNGA 2 cut(s) 10, 114
Hpy188III TCNNGA 3 cut(s) 40, 89, 128
Hpy8I GTNNAC 1 cut(s) 48
HpyAV CCTTC 1 cut(s) 421
HpyCH4IV ACGT 2 cut(s) 121, 140
HpyCH4V TGCA 2 cut(s) 252, 471
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 65, 249, 314, 437
HpySE526I ACGT 2 cut(s) 121, 140
Hsp92II CATG 2 cut(s) 256, 475
HspAI GCGC 1 cut(s) 24
KpnI GGTACC 1 cut(s) 529
Kzo9I GATC 1 cut(s) 130
LmnI GCTCC 4 cut(s) 173, 314, 326, 461
Lsp1109I GCAGC 1 cut(s) 188
LweI GCATC 2 cut(s) 14, 239
MaeII ACGT 2 cut(s) 121, 140
MaeIII GTNAC 2 cut(s) 136, 360
MalI GATC 1 cut(s) 132
MbiI CCGCTC 1 cut(s) 329
MboI GATC 1 cut(s) 130
MboII GAAGA 3 cut(s) 150, 294, 511
MflI RGATCY 1 cut(s) 130
MhlI GDGCHC 1 cut(s) 342
MluCI AATT 4 cut(s) 102, 116, 168, 447
MmeI TCCRAC 1 cut(s) 439
MnlI CCTC 3 cut(s) 378, 384, 500
Mph1103I ATGCAT 1 cut(s) 254
MroXI GAANNNNTTC 1 cut(s) 15
MseI TTAA 1 cut(s) 204
MslI CAYNNNNRTG 1 cut(s) 476
MspI CCGG 4 cut(s) 257, 444, 513, 529
MspR9I CCNGG 1 cut(s) 513
MwoI GCNNNNNNNGC 5 cut(s) 11, 65, 249, 314, 437
NciI CCSGG 1 cut(s) 513
NdeII GATC 1 cut(s) 130
NlaIII CATG 2 cut(s) 256, 475
NlaIV GGNNCC 1 cut(s) 527
NsbI TGCGCA 1 cut(s) 25
NsiI ATGCAT 1 cut(s) 254
NspI RCATGY 1 cut(s) 256
PaeI GCATGC 1 cut(s) 256
PdmI GAANNNNTTC 1 cut(s) 15
PfeI GAWTC 1 cut(s) 295
PinAI ACCGGT 1 cut(s) 528
PkrI GCNGC 1 cut(s) 178
Psp124BI GAGCTC 1 cut(s) 342
PspN4I GGNNCC 1 cut(s) 527
PstNI CAGNNNCTG 1 cut(s) 133
PsuI RGATCY 1 cut(s) 130
RsaI GTAC 1 cut(s) 527
RsaNI GTAC 1 cut(s) 526
RseI CAYNNNNRTG 1 cut(s) 476
SacI GAGCTC 1 cut(s) 342
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 1 cut(s) 177
Sau3AI GATC 1 cut(s) 130
ScrFI CCNGG 1 cut(s) 513
SduI GDGCHC 1 cut(s) 342
SfaNI GCATC 2 cut(s) 14, 239
SfcI CTRYAG 1 cut(s) 191
SmiMI CAYNNNNRTG 1 cut(s) 476
SmlI CTYRAG 1 cut(s) 388
SmoI CTYRAG 1 cut(s) 388
SphI GCATGC 1 cut(s) 256
Sse9I AATT 4 cut(s) 102, 116, 168, 447
SsiI CCGC 1 cut(s) 329
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 1 cut(s) 511
TaiI ACGT 2 cut(s) 124, 143
TaqI TCGA 3 cut(s) 90, 354, 505
TasI AATT 4 cut(s) 102, 116, 168, 447
TfiI GAWTC 1 cut(s) 295
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TseI GCWGC 1 cut(s) 176
TspGWI ACGGA 1 cut(s) 261
XapI RAATTY 1 cut(s) 447
XceI RCATGY 1 cut(s) 256
XmnI GAANNNNTTC 1 cut(s) 15
Zsp2I ATGCAT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.