MD14G1241700.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008137
Physical Location & Seq
Reverse (-)
32045956 .. 32046986
1031 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1241700.v1.1.491

Sequence Viewer

Length: 546 bp
ATGCCCTCGTCTAAAGCTATTCCTAACTCGATTCTGGTGCTGAGGATCTTCACTCTTTTATCATTAGTTGGTTCCGGGGTGCTGCTCGTTCTTGATAACTTCACACTTGAGGATGGTACCGAAACACATTTCTATGATATCCTTTCATTCAGGTTTATGCTCTCCACAGCAGCAATTGGAGCAGCATACACGCTATTTCAGTTACCTTTCGGTATATATTATGCCCTCACCGAAAAGAGGTTGATACGTAACGAATGCATGCCGCAGTTTGATTTCTACGGTGACAAGATTGACATGGAAATACGTACGTGTGCATGCATTGGTGAGCTTGCTTCTACCTTCGGGTGTGGGAGCTGGTTTTGGTTGCTGGCAGCACCAGGCGGCTTTCCAGAGTCAGAGTTGGATGAAGCACAATCCAAGAATAGTAGCTTCTTTGACAAGGCAATTATTGCTACTGGAATTTTGTTGTTTGGAGCCGTTTGTATGTCTGTCGTTTCAGTGCTTTCTTCCATCACTCGAACCTCCAACAGAGGTTACTTCGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

182

Amino Acids

19.89

Weight (kDa)

4.8

Isoelectric Point (pI)

52.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 11 - 97 3.8e-10 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 116
AccB1I GGYRCC 1 cut(s) 116
AciI CCGC 2 cut(s) 263, 381
AclWI GGATC 1 cut(s) 53
AcsI RAATTY 1 cut(s) 459
AfaI GTAC 2 cut(s) 118, 307
AfiI CCNNNNNNNGG 1 cut(s) 237
AflIII ACRYGT 1 cut(s) 308
AjnI CCWGG 1 cut(s) 376
AluBI AGCT 4 cut(s) 17, 328, 354, 429
AluI AGCT 4 cut(s) 17, 328, 354, 429
AlwI GGATC 1 cut(s) 53
ApeKI GCWGC 4 cut(s) 82, 170, 182, 371
ApoI RAATTY 1 cut(s) 459
Asp718I GGTACC 1 cut(s) 116
AsuC2I CCSGG 1 cut(s) 76
AsuHPI GGTGA 3 cut(s) 220, 293, 335
BanI GGYRCC 1 cut(s) 116
BbvCI CCTCAGC 1 cut(s) 41
BbvI GCAGC 4 cut(s) 69, 182, 194, 383
BccI CCATC 2 cut(s) 107, 518
BceAI ACGGC 1 cut(s) 461
BcgI CGANNNNNNTGC 2 cut(s) 19, 53
BciT130I CCWGG 1 cut(s) 378
BcnI CCSGG 1 cut(s) 76
BisI GCNGC 6 cut(s) 83, 171, 183, 263, 372, 382
BlsI GCNGC 6 cut(s) 84, 172, 184, 264, 373, 383
Bme1390I CCNGG 2 cut(s) 76, 378
BmiI GGNNCC 3 cut(s) 73, 118, 475
BmrFI CCNGG 2 cut(s) 76, 378
Bpu10I CCTNAGC 1 cut(s) 41
BpuEI CTTGAG 1 cut(s) 128
BpuMI CCSGG 1 cut(s) 76
BsaAI YACGTR 3 cut(s) 248, 305, 309
BsaJI CCNNGG 1 cut(s) 75
Bsc4I CCNNNNNNNGG 1 cut(s) 237
Bse1I ACTGG 1 cut(s) 460
BseBI CCWGG 1 cut(s) 378
BseDI CCNNGG 1 cut(s) 75
BseGI GGATG 2 cut(s) 118, 409
BseLI CCNNNNNNNGG 1 cut(s) 237
BseMII CTCAG 1 cut(s) 32
BseNI ACTGG 1 cut(s) 460
BseXI GCAGC 4 cut(s) 69, 182, 194, 383
BshNI GGYRCC 1 cut(s) 116
BsiSI CCGG 1 cut(s) 75
BsiWI CGTACG 1 cut(s) 305
BslI CCNNNNNNNGG 1 cut(s) 237
BsmI GAATGC 1 cut(s) 260
Bsp143I GATC 1 cut(s) 45
BspACI CCGC 2 cut(s) 263, 381
BspCNI CTCAG 1 cut(s) 33
BspLI GGNNCC 3 cut(s) 73, 118, 475
BspPI GGATC 1 cut(s) 53
BspT107I GGYRCC 1 cut(s) 116
BsrI ACTGG 1 cut(s) 460
BssECI CCNNGG 1 cut(s) 75
BssMI GATC 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 378
Bst4CI ACNGT 1 cut(s) 281
BstAPI GCANNNNNTGC 1 cut(s) 449
BstBAI YACGTR 3 cut(s) 248, 305, 309
BstC8I GCNNGC 4 cut(s) 260, 316, 330, 369
BstDEI CTNAG 1 cut(s) 41
BstF5I GGATG 2 cut(s) 118, 409
BstKTI GATC 1 cut(s) 48
BstMBI GATC 1 cut(s) 45
BstMWI GCNNNNNNNGC 2 cut(s) 179, 449
BstNI CCWGG 1 cut(s) 378
BstNSI RCATGY 2 cut(s) 262, 318
BstSCI CCNGG 2 cut(s) 74, 376
BstSNI TACGTA 2 cut(s) 248, 305
BstV1I GCAGC 4 cut(s) 69, 182, 194, 383
BstX2I RGATCY 1 cut(s) 45
BstYI RGATCY 1 cut(s) 45
BtsCI GGATG 2 cut(s) 118, 409
BtsIMutI CAGTG 1 cut(s) 504
Cac8I GCNNGC 4 cut(s) 260, 316, 330, 369
Csp6I GTAC 2 cut(s) 117, 306
CviAII CATG 3 cut(s) 259, 295, 315
CviJI RGCY 6 cut(s) 17, 328, 354, 384, 429, 476
CviKI_1 RGCY 6 cut(s) 17, 328, 354, 384, 429, 476
CviQI GTAC 2 cut(s) 117, 306
DdeI CTNAG 1 cut(s) 41
DpnI GATC 1 cut(s) 47
DpnII GATC 1 cut(s) 45
Eco105I TACGTA 2 cut(s) 248, 305
Eco32I GATATC 1 cut(s) 139
EcoRII CCWGG 1 cut(s) 376
EcoRV GATATC 1 cut(s) 139
EcoT22I ATGCAT 2 cut(s) 260, 320
FaeI CATG 3 cut(s) 262, 298, 318
FatI CATG 3 cut(s) 258, 294, 314
Fnu4HI GCNGC 6 cut(s) 83, 171, 183, 263, 372, 382
FokI GGATG 2 cut(s) 125, 416
Fsp4HI GCNGC 6 cut(s) 83, 171, 183, 263, 372, 382
GluI GCNGC 6 cut(s) 83, 171, 183, 263, 372, 382
HapII CCGG 1 cut(s) 75
Hin1II CATG 3 cut(s) 262, 298, 318
HinfI GANTC 2 cut(s) 31, 392
HpaII CCGG 1 cut(s) 75
HphI GGTGA 3 cut(s) 220, 293, 335
Hpy188I TCNGA 2 cut(s) 397, 542
Hpy188III TCNNGA 2 cut(s) 92, 389
HpyAV CCTTC 1 cut(s) 349
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4IV ACGT 3 cut(s) 247, 304, 308
HpyCH4V TGCA 3 cut(s) 258, 314, 318
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 449
HpyF3I CTNAG 1 cut(s) 41
HpySE526I ACGT 3 cut(s) 247, 304, 308
Hsp92II CATG 3 cut(s) 262, 298, 318
KpnI GGTACC 1 cut(s) 120
Kzo9I GATC 1 cut(s) 45
LmnI GCTCC 3 cut(s) 179, 351, 473
LpnPI CCDG 9 cut(s) 20, 88, 136, 340, 353, 363, 390, 402, 441
Lsp1109I GCAGC 4 cut(s) 69, 182, 194, 383
MaeII ACGT 3 cut(s) 247, 304, 308
MaeIII GTNAC 4 cut(s) 201, 248, 281, 533
MalI GATC 1 cut(s) 47
MboI GATC 1 cut(s) 45
MboII GAAGA 2 cut(s) 40, 498
MfeI CAATTG 1 cut(s) 174
MflI RGATCY 1 cut(s) 45
MluCI AATT 3 cut(s) 174, 444, 459
MlyI GAGTC 1 cut(s) 401
MmeI TCCRAC 1 cut(s) 381
MnlI CCTC 7 cut(s) 16, 36, 103, 231, 236, 524, 532
Mph1103I ATGCAT 2 cut(s) 260, 320
MslI CAYNNNNRTG 1 cut(s) 132
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 2 cut(s) 76, 378
MunI CAATTG 1 cut(s) 174
Mva1269I GAATGC 1 cut(s) 260
MvaI CCWGG 1 cut(s) 378
MwoI GCNNNNNNNGC 2 cut(s) 179, 449
NciI CCSGG 1 cut(s) 76
NdeII GATC 1 cut(s) 45
NlaIII CATG 3 cut(s) 262, 298, 318
NlaIV GGNNCC 3 cut(s) 73, 118, 475
NmuCI GTSAC 1 cut(s) 281
NsiI ATGCAT 2 cut(s) 260, 320
NspI RCATGY 2 cut(s) 262, 318
PaeI GCATGC 2 cut(s) 262, 318
PctI GAATGC 1 cut(s) 260
PfeI GAWTC 1 cut(s) 31
Pfl23II CGTACG 1 cut(s) 305
PkrI GCNGC 6 cut(s) 84, 172, 184, 264, 373, 383
PleI GAGTC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 400
Ppu21I YACGTR 3 cut(s) 248, 305, 309
Psp6I CCWGG 1 cut(s) 376
PspGI CCWGG 1 cut(s) 376
PspLI CGTACG 1 cut(s) 305
PspN4I GGNNCC 3 cut(s) 73, 118, 475
PsuI RGATCY 1 cut(s) 45
RsaI GTAC 2 cut(s) 118, 307
RsaNI GTAC 2 cut(s) 117, 306
RseI CAYNNNNRTG 1 cut(s) 132
SatI GCNGC 6 cut(s) 83, 171, 183, 263, 372, 382
Sau3AI GATC 1 cut(s) 45
SchI GAGTC 1 cut(s) 401
ScrFI CCNGG 2 cut(s) 76, 378
SmiMI CAYNNNNRTG 1 cut(s) 132
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
SnaBI TACGTA 2 cut(s) 248, 305
SphI GCATGC 2 cut(s) 262, 318
Sse9I AATT 3 cut(s) 174, 444, 459
SsiI CCGC 2 cut(s) 263, 381
StyD4I CCNGG 2 cut(s) 74, 376
TaaI ACNGT 1 cut(s) 281
TaiI ACGT 3 cut(s) 250, 307, 311
TaqI TCGA 2 cut(s) 29, 517
TasI AATT 3 cut(s) 174, 444, 459
TauI GCSGC 2 cut(s) 265, 384
TfiI GAWTC 1 cut(s) 31
TscAI CASTG 1 cut(s) 504
TseFI GTSAC 1 cut(s) 281
TseI GCWGC 4 cut(s) 82, 170, 182, 371
Tsp45I GTSAC 1 cut(s) 281
TspDTI ATGAA 2 cut(s) 135, 420
TspRI CASTG 1 cut(s) 504
XapI RAATTY 1 cut(s) 459
XceI RCATGY 2 cut(s) 262, 318
Zsp2I ATGCAT 2 cut(s) 260, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.