RLG00000005511

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0008137
Physical Location & Seq
Reverse (-)
68202547 .. 68205056
2510 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005511

Sequence Viewer

Length: 495 bp
ATGTCTAAAGCTATTATTCCTGCCCTAGTTCTTGTATTCAGGATCCTTACCCTTGCAGCCTGTGTTGCTTCTGGGCTGCTTCTCGTTCTTGACAACTTTAAACATGAGGATGGTGATAAACTACATTTTGATGACATCATTACATTCAGGTATGTGCTTTCTGCAGCAGCAATTGGAGCAGCATACAGCCTACTACAGTGCATGCCGGAGTTTGATTTCTATTTCGACAAGGTAGTGACCTTGGTTCTAGCTTCGGGTGTGGGAGCTGGTTTTGCAGCGAGATTTGAGCTCAAAAAGTTTTTCAAACTATTACTTCAAGTAGCGTCACTAGGAATTGACGACGACAACTTCGATCAAGAACTAGCCAAGTACAATGACTTCTTTGAGAAGGCAATTATTTCCAGCGGAATTCTCTTGGTTGGAGCTGTTTGCATGGCTGTAGTTTCGATTCTCACTTCCATCACTCGGACTTCAAATTCGGGTTTCTTCGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

165

Amino Acids

17.82

Weight (kDa)

5.03

Isoelectric Point (pI)

27.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 9 - 92 2.5e-08 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 405
AclWI GGATC 2 cut(s) 37, 50
AcsI RAATTY 2 cut(s) 408, 475
AfaI GTAC 1 cut(s) 371
AfiI CCNNNNNNNGG 1 cut(s) 465
AgsI TTSAA 3 cut(s) 304, 317, 474
AluBI AGCT 5 cut(s) 11, 251, 266, 289, 425
AluI AGCT 5 cut(s) 11, 251, 266, 289, 425
Alw21I GWGCWC 1 cut(s) 291
AlwI GGATC 2 cut(s) 37, 50
ApeKI GCWGC 6 cut(s) 56, 76, 164, 167, 179, 275
ApoI RAATTY 2 cut(s) 408, 475
ArsI GACNNNNNNTTYG 4 cut(s) 332, 364, 460, 492
AsuHPI GGTGA 1 cut(s) 125
BamHI GGATCC 1 cut(s) 42
BanII GRGCYC 1 cut(s) 291
BarI GAAGNNNNNNTAC 2 cut(s) 362, 394
Bbv12I GWGCWC 1 cut(s) 291
BbvI GCAGC 6 cut(s) 63, 68, 176, 179, 191, 287
BccI CCATC 2 cut(s) 104, 467
BfaI CTAG 4 cut(s) 26, 248, 329, 362
BfmI CTRYAG 3 cut(s) 162, 194, 438
BisI GCNGC 6 cut(s) 57, 77, 165, 168, 180, 276
BlsI GCNGC 6 cut(s) 58, 78, 166, 169, 181, 277
BmiI GGNNCC 1 cut(s) 44
BsaJI CCNNGG 1 cut(s) 240
Bsc4I CCNNNNNNNGG 1 cut(s) 465
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 1 cut(s) 115
BseLI CCNNNNNNNGG 1 cut(s) 465
BseXI GCAGC 6 cut(s) 63, 68, 176, 179, 191, 287
BsiHKAI GWGCWC 1 cut(s) 291
BsiSI CCGG 1 cut(s) 206
BslI CCNNNNNNNGG 1 cut(s) 465
Bsp1286I GDGCHC 1 cut(s) 291
Bsp143I GATC 2 cut(s) 42, 352
BspACI CCGC 1 cut(s) 405
BspLI GGNNCC 1 cut(s) 44
BspMAI CTGCAG 1 cut(s) 166
BspPI GGATC 2 cut(s) 37, 50
BssECI CCNNGG 1 cut(s) 240
BssMI GATC 2 cut(s) 42, 352
BssT1I CCWWGG 1 cut(s) 240
Bst4CI ACNGT 1 cut(s) 198
BstC8I GCNNGC 1 cut(s) 203
BstF5I GGATG 1 cut(s) 115
BstKTI GATC 2 cut(s) 45, 355
BstMBI GATC 2 cut(s) 42, 352
BstMWI GCNNNNNNNGC 3 cut(s) 65, 176, 272
BstNSI RCATGY 1 cut(s) 205
BstSFI CTRYAG 3 cut(s) 162, 194, 438
BstV1I GCAGC 6 cut(s) 63, 68, 176, 179, 191, 287
BstX2I RGATCY 1 cut(s) 42
BstYI RGATCY 1 cut(s) 42
BtsCI GGATG 1 cut(s) 115
BtsIMutI CAGTG 1 cut(s) 203
Cac8I GCNNGC 1 cut(s) 203
CseI GACGC 1 cut(s) 312
Csp6I GTAC 1 cut(s) 370
CviAII CATG 3 cut(s) 104, 202, 433
CviQI GTAC 1 cut(s) 370
DpnI GATC 2 cut(s) 44, 354
DpnII GATC 2 cut(s) 42, 352
DraI TTTAAA 1 cut(s) 100
Ecl136II GAGCTC 1 cut(s) 289
Eco130I CCWWGG 1 cut(s) 240
Eco24I GRGCYC 1 cut(s) 291
Eco53kI GAGCTC 1 cut(s) 289
EcoICRI GAGCTC 1 cut(s) 289
EcoRI GAATTC 1 cut(s) 408
EcoT14I CCWWGG 1 cut(s) 240
EcoT38I GRGCYC 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 3 cut(s) 107, 205, 436
FaiI YATR 5 cut(s) 105, 153, 184, 203, 434
FatI CATG 3 cut(s) 103, 201, 432
Fnu4HI GCNGC 6 cut(s) 57, 77, 165, 168, 180, 276
FokI GGATG 1 cut(s) 122
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 6 cut(s) 57, 77, 165, 168, 180, 276
FspBI CTAG 4 cut(s) 26, 248, 329, 362
GluI GCNGC 6 cut(s) 57, 77, 165, 168, 180, 276
HapII CCGG 1 cut(s) 206
HgaI GACGC 1 cut(s) 312
Hin1II CATG 3 cut(s) 107, 205, 436
HinfI GANTC 1 cut(s) 448
HpaII CCGG 1 cut(s) 206
HphI GGTGA 1 cut(s) 125
Hpy188I TCNGA 2 cut(s) 468, 491
Hpy188III TCNNGA 3 cut(s) 40, 89, 356
Hpy99I CGWCG 1 cut(s) 344
HpyAV CCTTC 1 cut(s) 382
HpyCH4III ACNGT 1 cut(s) 198
HpyCH4V TGCA 5 cut(s) 56, 164, 201, 275, 432
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 176, 272
Hsp92II CATG 3 cut(s) 107, 205, 436
Kzo9I GATC 2 cut(s) 42, 352
LmnI GCTCC 3 cut(s) 176, 263, 422
LpnPI CCDG 8 cut(s) 25, 33, 57, 73, 133, 219, 252, 415
Lsp1109I GCAGC 6 cut(s) 63, 68, 176, 179, 191, 287
MaeI CTAG 4 cut(s) 26, 248, 329, 362
MaeIII GTNAC 2 cut(s) 235, 324
MalI GATC 2 cut(s) 44, 354
MboI GATC 2 cut(s) 42, 352
MboII GAAGA 1 cut(s) 478
MfeI CAATTG 1 cut(s) 171
MflI RGATCY 1 cut(s) 42
MhlI GDGCHC 1 cut(s) 291
MluCI AATT 5 cut(s) 171, 333, 393, 408, 475
MmeI TCCRAC 1 cut(s) 400
MnlI CCTC 1 cut(s) 100
MseI TTAA 1 cut(s) 99
MslI CAYNNNNRTG 2 cut(s) 108, 129
MspA1I CMGCKG 1 cut(s) 405
MspI CCGG 1 cut(s) 206
MunI CAATTG 1 cut(s) 171
MwoI GCNNNNNNNGC 3 cut(s) 65, 176, 272
NdeII GATC 2 cut(s) 42, 352
NlaIII CATG 3 cut(s) 107, 205, 436
NlaIV GGNNCC 1 cut(s) 44
NmuCI GTSAC 2 cut(s) 235, 324
NspI RCATGY 1 cut(s) 205
PaeI GCATGC 1 cut(s) 205
PcsI WCGNNNNNNNCGW 1 cut(s) 348
PfeI GAWTC 1 cut(s) 448
PkrI GCNGC 6 cut(s) 58, 78, 166, 169, 181, 277
Psp124BI GAGCTC 1 cut(s) 291
PspN4I GGNNCC 1 cut(s) 44
PstI CTGCAG 1 cut(s) 166
PsuI RGATCY 1 cut(s) 42
RsaI GTAC 1 cut(s) 371
RsaNI GTAC 1 cut(s) 370
RseI CAYNNNNRTG 2 cut(s) 108, 129
SacI GAGCTC 1 cut(s) 291
SaqAI TTAA 1 cut(s) 99
SatI GCNGC 6 cut(s) 57, 77, 165, 168, 180, 276
Sau3AI GATC 2 cut(s) 42, 352
SduI GDGCHC 1 cut(s) 291
SetI ASST 8 cut(s) 13, 152, 234, 242, 253, 268, 291, 427
SfcI CTRYAG 3 cut(s) 162, 194, 438
SmiMI CAYNNNNRTG 2 cut(s) 108, 129
SphI GCATGC 1 cut(s) 205
Sse9I AATT 5 cut(s) 171, 333, 393, 408, 475
SsiI CCGC 1 cut(s) 405
SspMI CTAG 4 cut(s) 26, 248, 329, 362
SstI GAGCTC 1 cut(s) 291
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 1 cut(s) 198
TaqI TCGA 3 cut(s) 225, 351, 446
TasI AATT 5 cut(s) 171, 333, 393, 408, 475
TatI WGTACW 1 cut(s) 369
TfiI GAWTC 1 cut(s) 448
Tru1I TTAA 1 cut(s) 99
Tru9I TTAA 1 cut(s) 99
TscAI CASTG 1 cut(s) 203
TseFI GTSAC 2 cut(s) 235, 324
TseI GCWGC 6 cut(s) 56, 76, 164, 167, 179, 275
Tsp45I GTSAC 2 cut(s) 235, 324
TspRI CASTG 1 cut(s) 203
XapI RAATTY 2 cut(s) 408, 475
XceI RCATGY 1 cut(s) 205
XspI CTAG 4 cut(s) 26, 248, 329, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.