FvH4_5g18160

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007625
Physical Location & Seq
Forward (+)
10446359 .. 10450138
3780 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18160.t1

Sequence Viewer

Length: 2739 bp
ATGGCTTCTGTTCCAGCCTTGAAGCGCGCTGATTCAATTGCAGAAACTATGCCCGATGCTCTGAGACAGAGCCGCTACTACATGAAGAAAGTTTTTGCCAGGTTCGTTGGGGATGGGAAGAGGCTACTGAAGCCCCAGCATATAATGGAGGAAGTTGAAAATTCAATAGAGGATGGGAATGAAAAAAGCAAGGTGTTACGGGGATTACTTGGTTACATTCTCAATTCTACTCAGGAAGCAGCTGTTGTTCCGCCGTACGTTGCATTTTCTGTAAGACCAAATCCTGGTTTCTCAGAATTTGTTAAGGTGAATGCTGATGATCTAGCAGTGGATGGAATTTCCGTTACTGAATACTTGAAATTCAAGGAAATGATCTACGACGAGAACTGGGCAAATGATGAAAATGCTTTAGAAGTTGATTTTGGATCCATTGACTTCTCTCGTCCTCGCATGACTCTTCCTTCTTCCATTGGGAATGGACTTAACTTCATCCTCAAGTTAATCTCCTCAAGGCTGAATGCCACCTCATCCGATTATGCCAAACCCTTGCTTGGCTACTTGTTAGAACTCAATCACCACGGACAGAATCTTATGATCAACGAAACTATGGATTCGGTTGAGAAGCTTGAAACGTCATTGATTCGAGCACAATCGTTTGTCTCGGCACTCCCAAAAGAGACACCATTTCTTAATTTCGAGCAAAGGTTAAAGGATATGGGATTTGAGAAAGGATGGGGGGATACAGCAGAAAGAGTTAATGAGAGGATGTGGACACTGTCAGAAGTACTCCAAGCACCAGATTCTGTGAAGTTGGAGTCATTCTTTAGCAGCCTTCCTAATACATTCAACATTGTCATCTTCTCCCCTCACGGCTACTTTGGCCAGTCAGATGTCCTCGGATTGCCCGATACTGGTGGCCAGGTGGTTTATATACTTGATCAAGTTAGAGCCTTAGAGGAAGAGATGCTATTGAGAATAAAGCAGCAAGGGCTTGCTGTAAAGCCTCAGATTCTTGTGGTGACGCGACTCATACCAGACTCTCGAGGGACGAAGTGCAATGTGGAGTTGGAGGCTATAGTCAACACCAAGCACTCCCACATTCTCAGGGTTCCTTTCAGGACAGACAAAGGCATTCTGCGCCATTGGGTGTCCCGTTTTGATATCTACCCTTATCTCGAGACTTTTGTCCAGGATGCAACTGCAAAGATCTTACAACACATGGACTGCAAACCGGACCTCGTAATTGGGAACTACAGTGATGGAAACTTGGTGGCCTCTCTGATGGCTACCAAGCTTGGAATAACACAGGGAACAATTGCTCACGCTCTAGAAAAGACCAAGTATGAAGACTCGGATGCCAAATGGAAGGATTTTGATACAAAGTACCACTTTTCTTGTCAGTTCACTGCTGACTTGATCTCTATGAACTCAACTGATTTTATCATAACTAGCACATTTCAAGAAATATCAGGAGGTAAGGATCGACCTGGACAGTATGAAAGCCACACTGCATTCACAATGCCAGGACTCTGTCGAGTAGTCTCCGGCATTGATGTGTTTGACCCGAAGTTCAACATTGCTGCCCCCGGTGCTGACCAATCTGTCTACTTCCCCCATTCCGACAAACGGCAGCGCTTTACCAAATTTCATCCTTCCATTGAACAAATGGTCTACAATAAGGAGGAAAACGACGAGCACATAGGATTTTTGGCAGATAAAAAGAAACCAATCATATTTTCAATGGCAAGACTGGACAAAGTGAAGAACCTTTCAGGACTAGTTGAGTGGTTCGGAAGGAACAAGAGGCTGAGGAATTTGGTCAATCTTGTTATAGTAGGGGGCTTCTTTGACCCATCCAAGTCCAAAGACAGAGAGGAAATTGCTGAAATCATCAAATTGCATGCCTTGGTTAAAGAATATCAACTTAATGGCCAATTCAGATGGATAGCAGCTCAAACTGATAGATACCGCAACGGAGAGTTATACCGCTGCATTGCAGATACAAAGGGAGCTTTTGTGCAGCCTGCATTGTATGAAGCCTTTGGTCTTACAGTGATTGAGGCAATGAACTGTGGATTGCCCACATTTGCAACAAACCAAGGAGGCCCGGCTGAAATTATAGTTGATGGAGTCTCGGGATTCCATATTGATCCAAACAATGGTGAGGAATCCAGCAACAAGATTGCTGATTTCTTTGAGAGATGCAAGGCAGATGGTGAATATTGGAAGAAGATGTCTGCGGCGGGTCTGCAGCGTATTTATGAATGCTATACATGGAAAATATATGCAAACAAAGTGCTGAACATGGCATCCACTTACGGGTTTTGGAGGCAGTTGAGGAAGGAGCAGAAGCTAGCAAAGCAAAGCTACATTCATATGTTTTACAGTCTCCTATTTAGGACTTTGGCAAGGTCTGTACCTGTCCCAAGTGATGGATTTGAACCAGTACCAATGCCAAAGGCAATAGCTGCAGCTGACCATGGCCAGCCGAAGTCAGGAACAACTGTATCCAAACCCCCACAAGCCGCAGCTGCGCCACCACTGCCTCCAGTTGCTCTGATCACACCAACACCGAGGGATGGCGGAGGTTTGGAGCAGATAACTAAGCAACCTATTAGCCCTCCTGATGTTCCAAGTCCAAACCGCCCAACACGGTGCATTTGGAATTGCTTCTTCGTGATCTTTGCTTTTCTGTGCCTTGTTTATTACAAGATCAAGAACATGTACTATAGTCTATAA

Protein Analysis

913

Amino Acids

102.78

Weight (kDa)

6.83

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUS_N PF24861 9 - 123 1.9e-44 Sucrose synthase N-terminal
SUS_EPBD PF24862 159 - 247 3.7e-26 Sucrose synthase EPBD domain
GT-B_Sucrose_synth PF00862 270 - 558 5.5e-167 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 569 - 733 5.4e-30 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 576 - 731 5.9e-14 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1601
AccB7I CCANNNNNTGG 3 cut(s) 284, 2159, 2432
AccI GTMKAC 2 cut(s) 1605, 1671
AccII CGCG 2 cut(s) 27, 1024
AciI CCGC 9 cut(s) 73, 251, 1969, 1987, 2240, 2243, 2526, 2583, 2644
AclWI GGATC 4 cut(s) 420, 433, 1488, 2144
AcoI YGGCCR 4 cut(s) 880, 916, 1930, 2482
AcsI RAATTY 6 cut(s) 160, 296, 336, 359, 1643, 1813
AcuI CTGAAG 1 cut(s) 149
AfaI GTAC 6 cut(s) 257, 786, 1385, 2418, 2448, 2726
AfeI AGCGCT 1 cut(s) 1634
AfiI CCNNNNNNNGG 9 cut(s) 284, 551, 911, 1626, 2159, 2319, 2432, 2495, 2579
AflIII ACRYGT 1 cut(s) 2721
AhlI ACTAGT 1 cut(s) 1777
AjnI CCWGG 6 cut(s) 98, 283, 918, 1188, 1486, 1522
AjuI GAANNNNNNNTTGG 4 cut(s) 405, 437, 1720, 1752
AloI GAACNNNNNNTCC 2 cut(s) 2294, 2326
Alw21I GWGCWC 2 cut(s) 649, 1698
Alw26I GTCTC 7 cut(s) 58, 664, 671, 1172, 1546, 2137, 2393
AlwI GGATC 4 cut(s) 420, 433, 1488, 2144
AlwNI CAGNNNCTG 1 cut(s) 803
Ama87I CYCGRG 3 cut(s) 1041, 1175, 2134
Aor51HI AGCGCT 1 cut(s) 1634
AoxI GGCC 6 cut(s) 880, 916, 1272, 1930, 2104, 2482
ApoI RAATTY 6 cut(s) 160, 296, 336, 359, 1643, 1813
Asp700I GAANNNNTTC 1 cut(s) 2669
AspLEI GCGC 5 cut(s) 27, 29, 1140, 1635, 2536
AspS9I GGNCC 2 cut(s) 1234, 2105
AsuC2I CCSGG 2 cut(s) 1587, 2108
AsuHPI GGTGA 5 cut(s) 319, 566, 1030, 2174, 2228
AsuNHI GCTAGC 1 cut(s) 2353
AvaI CYCGRG 3 cut(s) 1041, 1175, 2134
AvaII GGWCC 1 cut(s) 1234
BaeI ACNNNNGTAYC 2 cut(s) 2490, 2523
BalI TGGCCA 4 cut(s) 882, 918, 1932, 2484
BamHI GGATCC 1 cut(s) 425
BbsI GAAGAC 1 cut(s) 1353
Bbv12I GWGCWC 2 cut(s) 649, 1698
BbvCI CCTCAGC 1 cut(s) 1808
BceAI ACGGC 3 cut(s) 238, 886, 1643
BciT130I CCWGG 6 cut(s) 100, 285, 920, 1190, 1488, 1524
BciVI GTATCC 2 cut(s) 733, 2518
BclI TGATCA 3 cut(s) 594, 937, 2559
BcnI CCSGG 2 cut(s) 1587, 2108
BcoDI GTCTC 7 cut(s) 58, 664, 671, 1172, 1546, 2137, 2393
BcuI ACTAGT 1 cut(s) 1777
BfaI CTAG 5 cut(s) 323, 1328, 1449, 1778, 2354
BfmI CTRYAG 5 cut(s) 1074, 1252, 2249, 2469, 2728
BfoI RGCGCY 1 cut(s) 1636
BfuI GTATCC 2 cut(s) 733, 2518
BglII AGATCT 1 cut(s) 1206
BmcAI AGTACT 1 cut(s) 786
Bme1390I CCNGG 8 cut(s) 100, 285, 920, 1190, 1488, 1524, 1587, 2108
Bme18I GGWCC 1 cut(s) 1234
BmeT110I CYCGRG 3 cut(s) 1041, 1175, 2134
BmgT120I GGNCC 2 cut(s) 1234, 2105
BmiI GGNNCC 2 cut(s) 427, 1110
BmrFI CCNGG 8 cut(s) 100, 285, 920, 1190, 1488, 1524, 1587, 2108
BmrI ACTGGG 1 cut(s) 397
BmsI GCATC 6 cut(s) 46, 954, 1183, 1345, 2192, 2318
BmtI GCTAGC 1 cut(s) 2357
BmuI ACTGGG 1 cut(s) 397
BoxI GACNNNNGTC 1 cut(s) 1184
BpiI GAAGAC 1 cut(s) 1353
BpmI CTGGAG 1 cut(s) 2532
Bpu10I CCTNAGC 1 cut(s) 1808
BpuEI CTTGAG 2 cut(s) 479, 493
BpuMI CCSGG 2 cut(s) 1587, 2108
BsaJI CCNNGG 7 cut(s) 577, 895, 1585, 1905, 2098, 2479, 2573
BsaWI WCCGGW 1 cut(s) 1231
BsaXI ACNNNNNCTCC 2 cut(s) 2001, 2031
Bsc4I CCNNNNNNNGG 9 cut(s) 284, 551, 911, 1626, 2159, 2319, 2432, 2495, 2579
Bse1I ACTGG 6 cut(s) 392, 883, 916, 1755, 2444, 2549
Bse3DI GCAATG 4 cut(s) 1063, 1575, 1992, 2070
BseBI CCWGG 6 cut(s) 100, 285, 920, 1190, 1488, 1524
BseDI CCNNGG 7 cut(s) 577, 895, 1585, 1905, 2098, 2479, 2573
BseLI CCNNNNNNNGG 9 cut(s) 284, 551, 911, 1626, 2159, 2319, 2432, 2495, 2579
BseMI GCAATG 4 cut(s) 1063, 1575, 1992, 2070
BseMII CTCAG 6 cut(s) 53, 245, 306, 1019, 1117, 1799
BseNI ACTGG 6 cut(s) 392, 883, 916, 1755, 2444, 2549
BsePI GCGCGC 1 cut(s) 25
BseRI GAGGAG 1 cut(s) 496
BseYI CCCAGC 1 cut(s) 135
BsgI GTGCAG 1 cut(s) 2039
Bsh1236I CGCG 2 cut(s) 27, 1024
BshFI GGCC 6 cut(s) 882, 918, 1274, 1932, 2106, 2484
BsiHKAI GWGCWC 2 cut(s) 649, 1698
BsiHKCI CYCGRG 3 cut(s) 1041, 1175, 2134
BsiSI CCGG 4 cut(s) 1232, 1545, 1587, 2108
BsiWI CGTACG 1 cut(s) 255
BslFI GGGAC 3 cut(s) 1060, 1135, 2408
BslI CCNNNNNNNGG 9 cut(s) 284, 551, 911, 1626, 2159, 2319, 2432, 2495, 2579
BsmAI GTCTC 7 cut(s) 58, 664, 671, 1172, 1546, 2137, 2393
BsmFI GGGAC 3 cut(s) 1060, 1135, 2408
BsmI GAATGC 5 cut(s) 316, 523, 1131, 1511, 2270
BsnI GGCC 6 cut(s) 882, 918, 1274, 1932, 2106, 2484
BsoBI CYCGRG 3 cut(s) 1041, 1175, 2134
Bsp1286I GDGCHC 2 cut(s) 649, 1698
Bsp19I CCATGG 1 cut(s) 2479
BspACI CCGC 9 cut(s) 73, 251, 1969, 1987, 2240, 2243, 2526, 2583, 2644
BspANI GGCC 6 cut(s) 882, 918, 1274, 1932, 2106, 2484
BspCNI CTCAG 6 cut(s) 54, 244, 305, 1018, 1116, 1800
BspFNI CGCG 2 cut(s) 27, 1024
BspLI GGNNCC 2 cut(s) 427, 1110
BspMAI CTGCAG 2 cut(s) 2253, 2473
BspOI GCTAGC 1 cut(s) 2357
BspPI GGATC 4 cut(s) 420, 433, 1488, 2144
BsrDI GCAATG 4 cut(s) 1063, 1575, 1992, 2070
BsrI ACTGG 6 cut(s) 392, 883, 916, 1755, 2444, 2549
BssECI CCNNGG 7 cut(s) 577, 895, 1585, 1905, 2098, 2479, 2573
BssHII GCGCGC 1 cut(s) 25
BssT1I CCWWGG 3 cut(s) 1905, 2098, 2479
Bst2UI CCWGG 6 cut(s) 100, 285, 920, 1190, 1488, 1524
Bst4CI ACNGT 8 cut(s) 777, 1256, 1494, 2053, 2072, 2387, 2506, 2655
Bst6I CTCTTC 3 cut(s) 113, 462, 954
BstAPI GCANNNNNTGC 1 cut(s) 2468
BstC8I GCNNGC 6 cut(s) 27, 993, 1902, 2025, 2355, 2486
BstDEI CTNAG 8 cut(s) 62, 231, 292, 952, 1005, 1103, 1808, 2604
BstDSI CCRYGG 2 cut(s) 577, 2479
BstFNI CGCG 2 cut(s) 27, 1024
BstH2I RGCGCY 1 cut(s) 1636
BstHHI GCGC 5 cut(s) 27, 29, 1140, 1635, 2536
BstMAI GTCTC 7 cut(s) 58, 664, 671, 1172, 1546, 2137, 2393
BstMWI GCNNNNNNNGC 9 cut(s) 130, 879, 988, 1137, 1589, 2359, 2468, 2531, 2542
BstNI CCWGG 6 cut(s) 100, 285, 920, 1190, 1488, 1524
BstNSI RCATGY 2 cut(s) 1904, 2725
BstPAI GACNNNNGTC 1 cut(s) 1184
BstSCI CCNGG 8 cut(s) 98, 283, 918, 1188, 1486, 1522, 1585, 2106
BstSFI CTRYAG 5 cut(s) 1074, 1252, 2249, 2469, 2728
BstUI CGCG 2 cut(s) 27, 1024
BstV2I GAAGAC 1 cut(s) 1353
BstX2I RGATCY 2 cut(s) 425, 1206
BstYI RGATCY 2 cut(s) 425, 1206
BsuI GTATCC 2 cut(s) 733, 2518
BsuRI GGCC 6 cut(s) 882, 918, 1274, 1932, 2106, 2484
BtgI CCRYGG 2 cut(s) 577, 2479
BtsI GCAGTG 4 cut(s) 333, 1404, 1506, 2540
BtsIMutI CAGTG 7 cut(s) 333, 773, 1261, 1404, 1506, 2058, 2540
Cac8I GCNNGC 6 cut(s) 27, 993, 1902, 2025, 2355, 2486
CaiI CAGNNNCTG 1 cut(s) 803
CfoI GCGC 5 cut(s) 27, 29, 1140, 1635, 2536
Cfr13I GGNCC 2 cut(s) 1234, 2105
CseI GACGC 1 cut(s) 1030
Csp6I GTAC 6 cut(s) 256, 785, 1384, 2417, 2447, 2725
CspCI CAANNNNNGTGG 2 cut(s) 1376, 1411
CviAII CATG 8 cut(s) 82, 451, 1219, 1901, 2274, 2305, 2480, 2722
CviQI GTAC 6 cut(s) 256, 785, 1384, 2417, 2447, 2725
DdeI CTNAG 8 cut(s) 62, 231, 292, 952, 1005, 1103, 1808, 2604
DrdI GACNNNNNNGTC 1 cut(s) 1601
DseDI GACNNNNNNGTC 1 cut(s) 1601
EaeI YGGCCR 4 cut(s) 880, 916, 1930, 2482
Eam1104I CTCTTC 3 cut(s) 113, 462, 954
EarI CTCTTC 3 cut(s) 113, 462, 954
EciI GGCGGA 2 cut(s) 240, 2598
Eco130I CCWWGG 3 cut(s) 1905, 2098, 2479
Eco32I GATATC 1 cut(s) 1162
Eco47I GGWCC 1 cut(s) 1234
Eco47III AGCGCT 1 cut(s) 1634
Eco57I CTGAAG 1 cut(s) 149
Eco88I CYCGRG 3 cut(s) 1041, 1175, 2134
EcoRII CCWGG 6 cut(s) 98, 283, 918, 1188, 1486, 1522
EcoRV GATATC 1 cut(s) 1162
EcoT14I CCWWGG 3 cut(s) 1905, 2098, 2479
ErhI CCWWGG 3 cut(s) 1905, 2098, 2479
FaeI CATG 8 cut(s) 85, 454, 1222, 1904, 2277, 2308, 2483, 2725
FalI AAGNNNNNCTT 2 cut(s) 1373, 1405
FaqI GGGAC 3 cut(s) 1060, 1135, 2408
FatI CATG 8 cut(s) 81, 450, 1218, 1900, 2273, 2304, 2479, 2721
FauI CCCGC 1 cut(s) 2236
FauNDI CATATG 1 cut(s) 2376
FbaI TGATCA 3 cut(s) 594, 937, 2559
FblI GTMKAC 2 cut(s) 1605, 1671
FspBI CTAG 5 cut(s) 323, 1328, 1449, 1778, 2354
GlaI GCGC 5 cut(s) 26, 28, 1139, 1634, 2535
GsaI CCCAGC 1 cut(s) 139
GsuI CTGGAG 1 cut(s) 2532
HaeII RGCGCY 1 cut(s) 1636
HaeIII GGCC 6 cut(s) 882, 918, 1274, 1932, 2106, 2484
HapII CCGG 4 cut(s) 1232, 1545, 1587, 2108
HgaI GACGC 1 cut(s) 1030
HhaI GCGC 5 cut(s) 27, 29, 1140, 1635, 2536
Hin1II CATG 8 cut(s) 85, 454, 1222, 1904, 2277, 2308, 2483, 2725
Hin6I GCGC 5 cut(s) 25, 27, 1138, 1633, 2534
HinP1I GCGC 5 cut(s) 25, 27, 1138, 1633, 2534
HincII GTYRAC 1 cut(s) 1081
HindII GTYRAC 1 cut(s) 1081
HindIII AAGCTT 2 cut(s) 623, 1292
HpaII CCGG 4 cut(s) 1232, 1545, 1587, 2108
HphI GGTGA 5 cut(s) 319, 566, 1030, 2174, 2228
Hpy166II GTNNAC 5 cut(s) 771, 1081, 1404, 1606, 1672
Hpy8I GTNNAC 5 cut(s) 771, 1081, 1404, 1606, 1672
Hpy99I CGWCG 2 cut(s) 383, 1694
HpyAV CCTTC 6 cut(s) 471, 842, 1360, 1662, 1788, 2335
HpyCH4III ACNGT 8 cut(s) 777, 1256, 1494, 2053, 2072, 2387, 2506, 2655
HpyCH4IV ACGT 2 cut(s) 258, 632
HpyF10VI GCNNNNNNNGC 9 cut(s) 130, 879, 988, 1137, 1589, 2359, 2468, 2531, 2542
HpyF3I CTNAG 8 cut(s) 62, 231, 292, 952, 1005, 1103, 1808, 2604
HpySE526I ACGT 2 cut(s) 258, 632
Hsp92II CATG 8 cut(s) 85, 454, 1222, 1904, 2277, 2308, 2483, 2725
HspAI GCGC 5 cut(s) 25, 27, 1138, 1633, 2534
Ksp22I TGATCA 3 cut(s) 594, 937, 2559
LmnI GCTCC 3 cut(s) 2009, 2344, 2593
LweI GCATC 6 cut(s) 46, 954, 1183, 1345, 2192, 2318
MaeI CTAG 5 cut(s) 323, 1328, 1449, 1778, 2354
MaeII ACGT 2 cut(s) 258, 632
MaeIII GTNAC 4 cut(s) 195, 212, 343, 1018
MfeI CAATTG 2 cut(s) 36, 1314
MflI RGATCY 2 cut(s) 425, 1206
MhlI GDGCHC 2 cut(s) 649, 1698
MlsI TGGCCA 4 cut(s) 882, 918, 1932, 2484
MluNI TGGCCA 4 cut(s) 882, 918, 1932, 2484
MlyI GAGTC 7 cut(s) 448, 824, 1020, 1031, 1343, 1521, 2139
MmeI TCCRAC 3 cut(s) 792, 1047, 1644
Mox20I TGGCCA 4 cut(s) 882, 918, 1932, 2484
MroXI GAANNNNTTC 1 cut(s) 2669
MscI TGGCCA 4 cut(s) 882, 918, 1932, 2484
MseI TTAA 8 cut(s) 303, 483, 500, 690, 707, 756, 1911, 1926
MslI CAYNNNNRTG 2 cut(s) 1553, 2375
Msp20I TGGCCA 4 cut(s) 882, 918, 1932, 2484
MspA1I CMGCKG 4 cut(s) 242, 1989, 2474, 2531
MspI CCGG 4 cut(s) 1232, 1545, 1587, 2108
MspR9I CCNGG 8 cut(s) 100, 285, 920, 1190, 1488, 1524, 1587, 2108
MunI CAATTG 2 cut(s) 36, 1314
Mva1269I GAATGC 5 cut(s) 316, 523, 1131, 1511, 2270
MvaI CCWGG 6 cut(s) 100, 285, 920, 1190, 1488, 1524
MvnI CGCG 2 cut(s) 27, 1024
MwoI GCNNNNNNNGC 9 cut(s) 130, 879, 988, 1137, 1589, 2359, 2468, 2531, 2542
NciI CCSGG 2 cut(s) 1587, 2108
NcoI CCATGG 1 cut(s) 2479
NdeI CATATG 1 cut(s) 2376
NheI GCTAGC 1 cut(s) 2353
NlaIII CATG 8 cut(s) 85, 454, 1222, 1904, 2277, 2308, 2483, 2725
NlaIV GGNNCC 2 cut(s) 427, 1110
NmeAIII GCCGAG 1 cut(s) 641
NmuCI GTSAC 1 cut(s) 1018
NspI RCATGY 2 cut(s) 1904, 2725
PaeI GCATGC 1 cut(s) 1904
PaeR7I CTCGAG 2 cut(s) 1041, 1175
PauI GCGCGC 1 cut(s) 25
PciI ACATGT 1 cut(s) 2721
PcsI WCGNNNNNNNCGW 1 cut(s) 903
PctI GAATGC 5 cut(s) 316, 523, 1131, 1511, 2270
PdmI GAANNNNTTC 1 cut(s) 2669
PfeI GAWTC 8 cut(s) 32, 586, 611, 640, 800, 1009, 2139, 2168
Pfl23II CGTACG 1 cut(s) 255
PflFI GACNNNGTC 2 cut(s) 775, 1530
PflMI CCANNNNNTGG 3 cut(s) 284, 2159, 2432
PfoI TCCNGGA 1 cut(s) 1188
PleI GAGTC 7 cut(s) 448, 823, 1020, 1031, 1343, 1521, 2138
PpsI GAGTC 7 cut(s) 448, 823, 1020, 1031, 1343, 1521, 2138
PscI ACATGT 1 cut(s) 2721
PshAI GACNNNNGTC 1 cut(s) 1184
Psp6I CCWGG 6 cut(s) 98, 283, 918, 1188, 1486, 1522
PspFI CCCAGC 1 cut(s) 135
PspGI CCWGG 6 cut(s) 98, 283, 918, 1188, 1486, 1522
PspLI CGTACG 1 cut(s) 255
PspN4I GGNNCC 2 cut(s) 427, 1110
PspPI GGNCC 2 cut(s) 1234, 2105
PstI CTGCAG 2 cut(s) 2253, 2473
PstNI CAGNNNCTG 1 cut(s) 803
PsuI RGATCY 2 cut(s) 425, 1206
PsyI GACNNNGTC 2 cut(s) 775, 1530
PteI GCGCGC 1 cut(s) 25
PvuII CAGCTG 3 cut(s) 242, 2474, 2531
RsaI GTAC 6 cut(s) 257, 786, 1385, 2418, 2448, 2726
RsaNI GTAC 6 cut(s) 256, 785, 1384, 2417, 2447, 2725
RseI CAYNNNNRTG 2 cut(s) 1553, 2375
SaqAI TTAA 8 cut(s) 303, 483, 500, 690, 707, 756, 1911, 1926
Sau96I GGNCC 2 cut(s) 1234, 2105
ScaI AGTACT 1 cut(s) 786
SchI GAGTC 7 cut(s) 448, 824, 1020, 1031, 1343, 1521, 2139
ScrFI CCNGG 8 cut(s) 100, 285, 920, 1190, 1488, 1524, 1587, 2108
SduI GDGCHC 2 cut(s) 649, 1698
SfaNI GCATC 6 cut(s) 46, 954, 1183, 1345, 2192, 2318
SfcI CTRYAG 5 cut(s) 1074, 1252, 2249, 2469, 2728
Sfr274I CTCGAG 2 cut(s) 1041, 1175
SinI GGWCC 1 cut(s) 1234
SlaI CTCGAG 2 cut(s) 1041, 1175
SmiMI CAYNNNNRTG 2 cut(s) 1553, 2375
SmlI CTYRAG 4 cut(s) 494, 508, 1041, 1175
SmoI CTYRAG 4 cut(s) 494, 508, 1041, 1175
SpeI ACTAGT 1 cut(s) 1777
SphI GCATGC 1 cut(s) 1904
SsiI CCGC 9 cut(s) 73, 251, 1969, 1987, 2240, 2243, 2526, 2583, 2644
SspI AATATT 1 cut(s) 2222
SspMI CTAG 5 cut(s) 323, 1328, 1449, 1778, 2354
StyD4I CCNGG 8 cut(s) 98, 283, 918, 1188, 1486, 1522, 1585, 2106
StyI CCWWGG 3 cut(s) 1905, 2098, 2479
TaaI ACNGT 8 cut(s) 777, 1256, 1494, 2053, 2072, 2387, 2506, 2655
TaiI ACGT 2 cut(s) 261, 635
TaqI TCGA 6 cut(s) 643, 696, 1042, 1176, 1483, 1534
TatI WGTACW 2 cut(s) 784, 2724
TauI GCSGC 3 cut(s) 75, 2243, 2528
TfiI GAWTC 8 cut(s) 32, 586, 611, 640, 800, 1009, 2139, 2168
Tru1I TTAA 8 cut(s) 303, 483, 500, 690, 707, 756, 1911, 1926
Tru9I TTAA 8 cut(s) 303, 483, 500, 690, 707, 756, 1911, 1926
TscAI CASTG 7 cut(s) 333, 780, 1261, 1411, 1513, 2058, 2547
TseFI GTSAC 1 cut(s) 1018
Tsp45I GTSAC 1 cut(s) 1018
TspGWI ACGGA 3 cut(s) 331, 594, 1989
TspRI CASTG 7 cut(s) 333, 780, 1261, 1411, 1513, 2058, 2547
Tth111I GACNNNGTC 2 cut(s) 775, 1530
Van91I CCANNNNNTGG 3 cut(s) 284, 2159, 2432
VpaK11BI GGWCC 1 cut(s) 1234
XapI RAATTY 6 cut(s) 160, 296, 336, 359, 1643, 1813
XbaI TCTAGA 1 cut(s) 1327
XceI RCATGY 2 cut(s) 1904, 2725
XcmI CCANNNNNNNNNTGG 1 cut(s) 1663
XhoI CTCGAG 2 cut(s) 1041, 1175
XmiI GTMKAC 2 cut(s) 1605, 1671
XmnI GAANNNNTTC 1 cut(s) 2669
XspI CTAG 5 cut(s) 323, 1328, 1449, 1778, 2354
ZrmI AGTACT 1 cut(s) 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.