RLG00000005538

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0007625
Physical Location & Seq
Forward (+)
68384166 .. 68388595
4430 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005538

Sequence Viewer

Length: 2514 bp
ATGCAGGAAGCAGCTGTTGTTCCGCCGCATGTTGCATTTTCTGTTAGACCAAATCCTGGTTTCTCTGAGTTTGTTAAGGTGAATGCTGATGATCTAGCAGTGGATGGAATCAGTGTTACCGAATACTTGAAATTCAAAGAAATGATCTACGACGAGAACTGGGCAAATGATGAAAATGCTTTGGAAATTGATTTTGGAGCCATTGACTTCTCTAGTCCTCGCATGACTCTTCCTTCTTCTATTGGGAATGGACTTAACTTCATCCTCAAGTTAATCTCCTCAAGGCTGAATGCCAGCTCATCCGATTATGCCAAACCCTTGCTTGACTACTTGTTAGCACTCAATCACCACGGACAGAATCTTATGATCAATGAAACTATGGATTCGGTGGAAAAGCTTGAAACGTCTTTGATTAGAGCACAATCGTTTGTCTCAGCACTCCCAAAAAACACACCATTTCTTAATTTCGAGCAGAGGTTAAAGGACATGGGTTTTGAGAAAGGATGGGGGGGTACAGCAGAAAGAGTTGGTGAGAGAATGAGGACGCTGTCAGAAGTACTCCAAGCACCGGATTGCGTGAAGTTGGAGTCATTCTTTAGCAGCCTTCCCAATACATTCAACATTGTCATATTCTCTCCTCACGGCTACTTTGGCCAGTCAGATGTCCTTGGATTGCCTGATACTGGTGGCCAGGTGGTTTATATACTTGATCAAGTGAGAGCCTTAGAGGAAGAGTTGCTACTGAGAATAAAGCAGCAAGGGCTTGCTGTGAAGCCTCAGATTCTTGTGGTGACGCGGCTCATACCAGACTCTCGAGGGACTAAGTGCAATGTGGAGTTGGAGGCTATAGTCAACACTAAGCACTCCCACATTCTCAGGGTTCCTTTCAGGACAGACAAAGGCATTCTGCGCCATTGGGTGTCCCGTTTTGATATCTACCCTTATCTTGAGACTTTTGTCCAGGATGCAACTGCAAAGATTCTAGAACACATGGACTGCAAACCGGACCTCATAATTGGGAACTACAGTGATGGAAACTTGGTGGCCTCTTTGATGGCTGCCAAGCTTGGAATAACACAGGGAACAATTGCCCACGCTCTAGAAAAGACCAAGTATGAAGACTCGGATGCCAAATGGAAGGAATTTGATACAAAGTACCACTTTTCTTGTCAGTTCACTGCAGACTTAATCTCGATGAACTCAACTGATTTTGTCATAACTAGCACATTTCAAGAAATAGCAGGAGGTAAGGATCGACCTGGACAGTATGAAAGCCACACCGCATTTACAATGCCAGGACTGTGTCGAGTAGTCTCAGGCATTGATGTGTTTGATCCGAAGTTCAACATTGCTGCTCCGGGTGCTGACCAATCTGTCTACTTCCCCAACTCCGAAAAACAGAAGCGTTTTACCAAATTTCATCCTTCCATTGAACAAATGGTCTACAGTAAGGAGGAAAACGACGAGCACATAGGATTTTTGGCAGACAAAAAGAAACCAATCATCTTTTCAATGGCAAGACTGGATAAAGTGAAGAACCTCTCAGGACTAGTTGAGTGGTTCGGAAAGAACAAGAGGCTGAGGAATTTGGTCAACCTTGTTATAGTAGGGGGGTTCTTTGACCCATCCAAGTCCAAAGACAGAGAGGAAATTGCTGAAATTATCAAACTGCATGCCTTGGTTAAAGAATATCAACTTAATGGCCAGTTCAGATGGATAGCAGCTCAAACTGATAGGTATCGCAACGGAGAGTTGTACCGCTGCATTGCTGATACAAAGGGAGCTTTTGTGCAGCCTGCATTGTATGAAGCCTTCGGTCTTACAGTGATTGAGGCAATGAACTGTGGATTGCCCTCTTTTGCTACAAACCAAGGAGGCCCGGCTGAAATTATTGTTGATGGAGTCTCGGGCTTCCATATTGATCCCAACAATGGTGAAGAGTCCAGCAACAAGATTGCTGATTTCTTTGAGAGATGCAGGGCAGATGGTGAATACTGGAAGAAGATGTCCGCGGCGGGTCTGCAGCGTATATATGCATGCTATACGTGGAAAATATATGCAAACAAAATGTTGAACATGGGATCCACTTATGGGTTTTGGAGGCAGTTGAGGAAGGAGCCGAAGCTAGCAAAGCAAAGCTACATTCATATGTTTTACAGTCTCCTATTTAGGACTTTGGCAAGGACTGTCCCTGTCCCAAGTGATGGATATGAACCAGTACCGACGCCAAAGGCAATAGCTGCAGCTGACCAAGGCAAACCAAAGTCAGTGACAACTGTATCCAAACCCCCACAAGCCGCAGCTGCACCAACAATGCCAACAGTTCCAGTTGCTCGGATCACACCAACACCAAGGGATGAAGGAGGTTTAGAGCAGATAAATAAGCAACCTGTTGGCCCTCCTGATGTTACAAGTCCAAACAGCCCAACACGGTGCCTTTGGAATTGCTTCTTCGTCATCATTGGTTTTCTGTGCCTTGTTTATTACAAGCTCAAGAATATGTACTACAAATGA

Protein Analysis

838

Amino Acids

93.92

Weight (kDa)

6.78

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUS_N PF24861 1 - 47 4.1e-18 Sucrose synthase N-terminal
SUS_EPBD PF24862 83 - 170 1.3e-26 Sucrose synthase EPBD domain
GT-B_Sucrose_synth PF00862 194 - 482 1.1e-167 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 493 - 657 1.5e-29 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 500 - 655 9.4e-14 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1373
AccB1I GGYRCC 1 cut(s) 2433
AccB7I CCANNNNNTGG 2 cut(s) 56, 2204
AccI GTMKAC 2 cut(s) 1377, 1443
AccII CGCG 2 cut(s) 796, 2012
AciI CCGC 9 cut(s) 23, 26, 796, 1281, 1759, 2010, 2012, 2015, 2298
AclWI GGATC 6 cut(s) 1260, 1328, 1916, 2076, 2089, 2345
AcoI YGGCCR 3 cut(s) 652, 688, 1702
AcsI RAATTY 4 cut(s) 131, 1142, 1415, 1585
AcyI GRCGYC 1 cut(s) 2225
AfaI GTAC 6 cut(s) 514, 558, 1157, 1757, 2220, 2504
AfiI CCNNNNNNNGG 6 cut(s) 56, 568, 683, 1931, 2091, 2204
AgsI TTSAA 9 cut(s) 130, 136, 401, 619, 1232, 1345, 1433, 1512, 2074
AhlI ACTAGT 1 cut(s) 1549
AjnI CCWGG 5 cut(s) 55, 690, 960, 1258, 1294
AjuI GAANNNNNNNTTGG 4 cut(s) 177, 209, 1492, 1524
AloI GAACNNNNNNTCC 2 cut(s) 2066, 2098
Alw21I GWGCWC 2 cut(s) 421, 1470
Alw26I GTCTC 5 cut(s) 436, 944, 1318, 1909, 2165
AlwI GGATC 6 cut(s) 1260, 1328, 1916, 2076, 2089, 2345
Ama87I CYCGRG 2 cut(s) 813, 1906
AoxI GGCC 6 cut(s) 652, 688, 1044, 1702, 1876, 2395
ApoI RAATTY 4 cut(s) 131, 1142, 1415, 1585
ArsI GACNNNNNNTTYG 2 cut(s) 476, 508
Asp700I GAANNNNTTC 1 cut(s) 2447
AspLEI GCGC 1 cut(s) 912
AspS9I GGNCC 3 cut(s) 1006, 1877, 2396
AsuC2I CCSGG 2 cut(s) 1359, 1880
AsuHPI GGTGA 6 cut(s) 91, 338, 542, 802, 1946, 2000
AsuNHI GCTAGC 1 cut(s) 2125
AvaI CYCGRG 2 cut(s) 813, 1906
AvaII GGWCC 1 cut(s) 1006
BaeI ACNNNNGTAYC 2 cut(s) 2262, 2295
BalI TGGCCA 3 cut(s) 654, 690, 1704
BamHI GGATCC 1 cut(s) 2081
BanI GGYRCC 1 cut(s) 2433
BarI GAAGNNNNNNTAC 4 cut(s) 723, 755, 1796, 1828
BbsI GAAGAC 1 cut(s) 1125
Bbv12I GWGCWC 2 cut(s) 421, 1470
BbvCI CCTCAGC 1 cut(s) 1580
BccI CCATC 9 cut(s) 98, 498, 1025, 1048, 1633, 1707, 1892, 1979, 2198
BceAI ACGGC 1 cut(s) 658
BciT130I CCWGG 5 cut(s) 57, 692, 962, 1260, 1296
BciVI GTATCC 1 cut(s) 2290
BclI TGATCA 2 cut(s) 366, 709
BcnI CCSGG 2 cut(s) 1359, 1880
BcoDI GTCTC 5 cut(s) 436, 944, 1318, 1909, 2165
BcuI ACTAGT 1 cut(s) 1549
BfaI CTAG 7 cut(s) 95, 213, 983, 1100, 1221, 1550, 2126
BfmI CTRYAG 6 cut(s) 846, 1024, 1179, 1444, 2021, 2241
BfuI GTATCC 1 cut(s) 2290
BmcAI AGTACT 1 cut(s) 558
Bme1390I CCNGG 7 cut(s) 57, 692, 962, 1260, 1296, 1359, 1880
Bme18I GGWCC 1 cut(s) 1006
BmeT110I CYCGRG 2 cut(s) 813, 1906
BmgT120I GGNCC 3 cut(s) 1006, 1877, 2396
BmiI GGNNCC 5 cut(s) 199, 882, 2083, 2118, 2435
BmrFI CCNGG 7 cut(s) 57, 692, 962, 1260, 1296, 1359, 1880
BmrI ACTGGG 1 cut(s) 169
BmsI GCATC 3 cut(s) 955, 1117, 1964
BmtI GCTAGC 1 cut(s) 2129
BmuI ACTGGG 1 cut(s) 169
BoxI GACNNNNGTC 1 cut(s) 956
BpiI GAAGAC 1 cut(s) 1125
Bpu10I CCTNAGC 1 cut(s) 1580
BpuEI CTTGAG 4 cut(s) 251, 265, 968, 2477
BpuMI CCSGG 2 cut(s) 1359, 1880
BsaAI YACGTR 1 cut(s) 2046
BsaBI GATNNNNATC 1 cut(s) 1737
BsaHI GRCGYC 1 cut(s) 2225
BsaJI CCNNGG 7 cut(s) 349, 667, 1677, 1870, 2010, 2251, 2351
BsaWI WCCGGW 2 cut(s) 568, 1003
BsaXI ACNNNNNCTCC 2 cut(s) 1773, 1803
Bsc4I CCNNNNNNNGG 6 cut(s) 56, 568, 683, 1931, 2091, 2204
Bse1I ACTGG 8 cut(s) 164, 655, 688, 1527, 1705, 2000, 2216, 2327
Bse3DI GCAATG 4 cut(s) 835, 1347, 1764, 1842
Bse8I GATNNNNATC 1 cut(s) 1737
BseBI CCWGG 5 cut(s) 57, 692, 962, 1260, 1296
BseDI CCNNGG 7 cut(s) 349, 667, 1677, 1870, 2010, 2251, 2351
BseGI GGATG 9 cut(s) 109, 261, 299, 509, 970, 1132, 1420, 1625, 2362
BseJI GATNNNNATC 1 cut(s) 1737
BseLI CCNNNNNNNGG 6 cut(s) 56, 568, 683, 1931, 2091, 2204
BseMI GCAATG 4 cut(s) 835, 1347, 1764, 1842
BseMII CTCAG 8 cut(s) 57, 447, 734, 791, 889, 1329, 1557, 1571
BseNI ACTGG 8 cut(s) 164, 655, 688, 1527, 1705, 2000, 2216, 2327
BseRI GAGGAG 2 cut(s) 268, 627
BsgI GTGCAG 2 cut(s) 1811, 2289
Bsh1236I CGCG 2 cut(s) 796, 2012
BshFI GGCC 6 cut(s) 654, 690, 1046, 1704, 1878, 2397
BshNI GGYRCC 1 cut(s) 2433
BsiHKAI GWGCWC 2 cut(s) 421, 1470
BsiHKCI CYCGRG 2 cut(s) 813, 1906
BsiSI CCGG 4 cut(s) 569, 1004, 1358, 1880
BslFI GGGAC 4 cut(s) 832, 907, 2174, 2180
BslI CCNNNNNNNGG 6 cut(s) 56, 568, 683, 1931, 2091, 2204
BsmAI GTCTC 5 cut(s) 436, 944, 1318, 1909, 2165
BsmFI GGGAC 4 cut(s) 832, 907, 2174, 2180
BsmI GAATGC 3 cut(s) 88, 295, 903
BsnI GGCC 6 cut(s) 654, 690, 1046, 1704, 1878, 2397
BsoBI CYCGRG 2 cut(s) 813, 1906
Bsp1286I GDGCHC 2 cut(s) 421, 1470
Bsp143I GATC 9 cut(s) 91, 144, 366, 709, 1252, 1333, 1921, 2081, 2337
BspACI CCGC 9 cut(s) 23, 26, 796, 1281, 1759, 2010, 2012, 2015, 2298
BspANI GGCC 6 cut(s) 654, 690, 1046, 1704, 1878, 2397
BspCNI CTCAG 8 cut(s) 58, 446, 735, 790, 888, 1328, 1556, 1572
BspFNI CGCG 2 cut(s) 796, 2012
BspLI GGNNCC 5 cut(s) 199, 882, 2083, 2118, 2435
BspMAI CTGCAG 3 cut(s) 1183, 2025, 2245
BspOI GCTAGC 1 cut(s) 2129
BspPI GGATC 6 cut(s) 1260, 1328, 1916, 2076, 2089, 2345
BspT107I GGYRCC 1 cut(s) 2433
BsrDI GCAATG 4 cut(s) 835, 1347, 1764, 1842
BsrI ACTGG 8 cut(s) 164, 655, 688, 1527, 1705, 2000, 2216, 2327
BssECI CCNNGG 7 cut(s) 349, 667, 1677, 1870, 2010, 2251, 2351
BssMI GATC 9 cut(s) 91, 144, 366, 709, 1252, 1333, 1921, 2081, 2337
BssNI GRCGYC 1 cut(s) 2225
BssT1I CCWWGG 5 cut(s) 667, 1677, 1870, 2251, 2351
Bst2UI CCWGG 5 cut(s) 57, 692, 962, 1260, 1296
Bst6I CTCTTC 3 cut(s) 234, 726, 1932
BstACI GRCGYC 1 cut(s) 2225
BstAPI GCANNNNNTGC 1 cut(s) 2240
BstBAI YACGTR 1 cut(s) 2046
BstC8I GCNNGC 6 cut(s) 295, 765, 1674, 1797, 2038, 2127
BstDSI CCRYGG 2 cut(s) 349, 2010
BstF5I GGATG 9 cut(s) 109, 261, 299, 509, 970, 1132, 1420, 1625, 2362
BstFNI CGCG 2 cut(s) 796, 2012
BstHHI GCGC 1 cut(s) 912
BstKTI GATC 9 cut(s) 94, 147, 369, 712, 1255, 1336, 1924, 2084, 2340
BstMAI GTCTC 5 cut(s) 436, 944, 1318, 1909, 2165
BstMBI GATC 9 cut(s) 91, 144, 366, 709, 1252, 1333, 1921, 2081, 2337
BstMWI GCNNNNNNNGC 7 cut(s) 651, 760, 909, 1361, 2131, 2240, 2303
BstNI CCWGG 5 cut(s) 57, 692, 962, 1260, 1296
BstNSI RCATGY 3 cut(s) 32, 1676, 2040
BstPAI GACNNNNGTC 1 cut(s) 956
BstSCI CCNGG 7 cut(s) 55, 690, 960, 1258, 1294, 1357, 1878
BstSFI CTRYAG 6 cut(s) 846, 1024, 1179, 1444, 2021, 2241
BstUI CGCG 2 cut(s) 796, 2012
BstV2I GAAGAC 1 cut(s) 1125
BstX2I RGATCY 1 cut(s) 2081
BstYI RGATCY 1 cut(s) 2081
BsuI GTATCC 1 cut(s) 2290
BsuRI GGCC 6 cut(s) 654, 690, 1046, 1704, 1878, 2397
BtgI CCRYGG 2 cut(s) 349, 2010
BtsCI GGATG 9 cut(s) 109, 261, 299, 509, 970, 1132, 1420, 1625, 2362
BtsI GCAGTG 2 cut(s) 105, 1176
BtsIMutI CAGTG 6 cut(s) 105, 118, 1033, 1176, 1830, 2274
Cac8I GCNNGC 6 cut(s) 295, 765, 1674, 1797, 2038, 2127
CfoI GCGC 1 cut(s) 912
Cfr13I GGNCC 3 cut(s) 1006, 1877, 2396
Cfr42I CCGCGG 1 cut(s) 2013
CseI GACGC 3 cut(s) 553, 802, 2233
Csp6I GTAC 6 cut(s) 513, 557, 1156, 1756, 2219, 2503
CspCI CAANNNNNGTGG 2 cut(s) 1148, 1183
CviAII CATG 7 cut(s) 29, 223, 487, 991, 1673, 2037, 2077
CviQI GTAC 6 cut(s) 513, 557, 1156, 1756, 2219, 2503
DpnI GATC 9 cut(s) 93, 146, 368, 711, 1254, 1335, 1923, 2083, 2339
DpnII GATC 9 cut(s) 91, 144, 366, 709, 1252, 1333, 1921, 2081, 2337
DrdI GACNNNNNNGTC 1 cut(s) 1373
DseDI GACNNNNNNGTC 1 cut(s) 1373
EaeI YGGCCR 3 cut(s) 652, 688, 1702
Eam1104I CTCTTC 3 cut(s) 234, 726, 1932
EarI CTCTTC 3 cut(s) 234, 726, 1932
EciI GGCGGA 1 cut(s) 12
Eco130I CCWWGG 5 cut(s) 667, 1677, 1870, 2251, 2351
Eco32I GATATC 1 cut(s) 934
Eco47I GGWCC 1 cut(s) 1006
Eco88I CYCGRG 2 cut(s) 813, 1906
EcoRII CCWGG 5 cut(s) 55, 690, 960, 1258, 1294
EcoRV GATATC 1 cut(s) 934
EcoT14I CCWWGG 5 cut(s) 667, 1677, 1870, 2251, 2351
EcoT22I ATGCAT 1 cut(s) 2038
ErhI CCWWGG 5 cut(s) 667, 1677, 1870, 2251, 2351
FaeI CATG 7 cut(s) 32, 226, 490, 994, 1676, 2040, 2080
FalI AAGNNNNNCTT 2 cut(s) 1145, 1177
FaqI GGGAC 4 cut(s) 832, 907, 2174, 2180
FatI CATG 7 cut(s) 28, 222, 486, 990, 1672, 2036, 2076
FauI CCCGC 1 cut(s) 2008
FauNDI CATATG 1 cut(s) 2148
FbaI TGATCA 2 cut(s) 366, 709
FblI GTMKAC 2 cut(s) 1377, 1443
FokI GGATG 9 cut(s) 116, 248, 286, 516, 977, 1139, 1407, 1612, 2369
FspBI CTAG 7 cut(s) 95, 213, 983, 1100, 1221, 1550, 2126
GlaI GCGC 1 cut(s) 911
HaeIII GGCC 6 cut(s) 654, 690, 1046, 1704, 1878, 2397
HapII CCGG 4 cut(s) 569, 1004, 1358, 1880
HgaI GACGC 3 cut(s) 553, 802, 2233
HhaI GCGC 1 cut(s) 912
Hin1I GRCGYC 1 cut(s) 2225
Hin1II CATG 7 cut(s) 32, 226, 490, 994, 1676, 2040, 2080
Hin6I GCGC 1 cut(s) 910
HinP1I GCGC 1 cut(s) 910
HincII GTYRAC 2 cut(s) 853, 1594
HindII GTYRAC 2 cut(s) 853, 1594
HindIII AAGCTT 2 cut(s) 395, 1064
HpaII CCGG 4 cut(s) 569, 1004, 1358, 1880
HphI GGTGA 6 cut(s) 91, 338, 542, 802, 1946, 2000
Hpy166II GTNNAC 5 cut(s) 853, 1176, 1378, 1444, 1594
Hpy8I GTNNAC 5 cut(s) 853, 1176, 1378, 1444, 1594
Hpy99I CGWCG 3 cut(s) 155, 1466, 2227
HpyAV CCTTC 7 cut(s) 243, 614, 1132, 1434, 1822, 2107, 2354
HpyCH4IV ACGT 2 cut(s) 404, 2045
HpyF10VI GCNNNNNNNGC 7 cut(s) 651, 760, 909, 1361, 2131, 2240, 2303
HpySE526I ACGT 2 cut(s) 404, 2045
Hsp92I GRCGYC 1 cut(s) 2225
Hsp92II CATG 7 cut(s) 32, 226, 490, 994, 1676, 2040, 2080
HspAI GCGC 1 cut(s) 910
Ksp22I TGATCA 2 cut(s) 366, 709
KspI CCGCGG 1 cut(s) 2013
Kzo9I GATC 9 cut(s) 91, 144, 366, 709, 1252, 1333, 1921, 2081, 2337
LmnI GCTCC 4 cut(s) 197, 1360, 1781, 2116
LweI GCATC 3 cut(s) 955, 1117, 1964
MaeI CTAG 7 cut(s) 95, 213, 983, 1100, 1221, 1550, 2126
MaeII ACGT 2 cut(s) 404, 2045
MaeIII GTNAC 4 cut(s) 115, 790, 2269, 2407
MalI GATC 9 cut(s) 93, 146, 368, 711, 1254, 1335, 1923, 2083, 2339
MboI GATC 9 cut(s) 91, 144, 366, 709, 1252, 1333, 1921, 2081, 2337
MboII GAAGA 9 cut(s) 221, 228, 743, 1130, 1546, 1949, 2011, 2014, 2443
MfeI CAATTG 1 cut(s) 1086
MflI RGATCY 1 cut(s) 2081
MhlI GDGCHC 2 cut(s) 421, 1470
MlsI TGGCCA 3 cut(s) 654, 690, 1704
MluNI TGGCCA 3 cut(s) 654, 690, 1704
MlyI GAGTC 6 cut(s) 220, 596, 803, 1115, 1911, 1949
MmeI TCCRAC 2 cut(s) 564, 819
Mox20I TGGCCA 3 cut(s) 654, 690, 1704
Mph1103I ATGCAT 1 cut(s) 2038
MroXI GAANNNNTTC 1 cut(s) 2447
MscI TGGCCA 3 cut(s) 654, 690, 1704
MseI TTAA 8 cut(s) 75, 255, 272, 462, 479, 1187, 1683, 1698
MslI CAYNNNNRTG 2 cut(s) 1325, 2147
Msp20I TGGCCA 3 cut(s) 654, 690, 1704
MspA1I CMGCKG 5 cut(s) 14, 1761, 2012, 2246, 2303
MspI CCGG 4 cut(s) 569, 1004, 1358, 1880
MspR9I CCNGG 7 cut(s) 57, 692, 962, 1260, 1296, 1359, 1880
MunI CAATTG 1 cut(s) 1086
Mva1269I GAATGC 3 cut(s) 88, 295, 903
MvaI CCWGG 5 cut(s) 57, 692, 962, 1260, 1296
MvnI CGCG 2 cut(s) 796, 2012
MwoI GCNNNNNNNGC 7 cut(s) 651, 760, 909, 1361, 2131, 2240, 2303
NciI CCSGG 2 cut(s) 1359, 1880
NdeI CATATG 1 cut(s) 2148
NdeII GATC 9 cut(s) 91, 144, 366, 709, 1252, 1333, 1921, 2081, 2337
NheI GCTAGC 1 cut(s) 2125
NlaIII CATG 7 cut(s) 32, 226, 490, 994, 1676, 2040, 2080
NlaIV GGNNCC 5 cut(s) 199, 882, 2083, 2118, 2435
NmuCI GTSAC 2 cut(s) 790, 2269
NsiI ATGCAT 1 cut(s) 2038
NspI RCATGY 3 cut(s) 32, 1676, 2040
PaeI GCATGC 2 cut(s) 1676, 2040
PaeR7I CTCGAG 1 cut(s) 813
PctI GAATGC 3 cut(s) 88, 295, 903
PdmI GAANNNNTTC 1 cut(s) 2447
PfeI GAWTC 5 cut(s) 108, 358, 383, 781, 979
PflFI GACNNNGTC 2 cut(s) 547, 1302
PflMI CCANNNNNTGG 2 cut(s) 56, 2204
PfoI TCCNGGA 1 cut(s) 960
PleI GAGTC 6 cut(s) 220, 595, 803, 1115, 1910, 1948
PpsI GAGTC 6 cut(s) 220, 595, 803, 1115, 1910, 1948
Ppu21I YACGTR 1 cut(s) 2046
PshAI GACNNNNGTC 1 cut(s) 956
Psp6I CCWGG 5 cut(s) 55, 690, 960, 1258, 1294
PspGI CCWGG 5 cut(s) 55, 690, 960, 1258, 1294
PspN4I GGNNCC 5 cut(s) 199, 882, 2083, 2118, 2435
PspPI GGNCC 3 cut(s) 1006, 1877, 2396
PstI CTGCAG 3 cut(s) 1183, 2025, 2245
PsuI RGATCY 1 cut(s) 2081
PsyI GACNNNGTC 2 cut(s) 547, 1302
PvuII CAGCTG 3 cut(s) 14, 2246, 2303
RsaI GTAC 6 cut(s) 514, 558, 1157, 1757, 2220, 2504
RsaNI GTAC 6 cut(s) 513, 557, 1156, 1756, 2219, 2503
RseI CAYNNNNRTG 2 cut(s) 1325, 2147
SacII CCGCGG 1 cut(s) 2013
SaqAI TTAA 8 cut(s) 75, 255, 272, 462, 479, 1187, 1683, 1698
Sau3AI GATC 9 cut(s) 91, 144, 366, 709, 1252, 1333, 1921, 2081, 2337
Sau96I GGNCC 3 cut(s) 1006, 1877, 2396
ScaI AGTACT 1 cut(s) 558
SchI GAGTC 6 cut(s) 220, 596, 803, 1115, 1911, 1949
ScrFI CCNGG 7 cut(s) 57, 692, 962, 1260, 1296, 1359, 1880
SduI GDGCHC 2 cut(s) 421, 1470
SfaNI GCATC 3 cut(s) 955, 1117, 1964
SfcI CTRYAG 6 cut(s) 846, 1024, 1179, 1444, 2021, 2241
Sfr274I CTCGAG 1 cut(s) 813
Sfr303I CCGCGG 1 cut(s) 2013
SgrBI CCGCGG 1 cut(s) 2013
SinI GGWCC 1 cut(s) 1006
SlaI CTCGAG 1 cut(s) 813
SmiMI CAYNNNNRTG 2 cut(s) 1325, 2147
SmlI CTYRAG 5 cut(s) 266, 280, 813, 947, 2492
SmoI CTYRAG 5 cut(s) 266, 280, 813, 947, 2492
SpeI ACTAGT 1 cut(s) 1549
SphI GCATGC 2 cut(s) 1676, 2040
SsiI CCGC 9 cut(s) 23, 26, 796, 1281, 1759, 2010, 2012, 2015, 2298
SspMI CTAG 7 cut(s) 95, 213, 983, 1100, 1221, 1550, 2126
StyD4I CCNGG 7 cut(s) 55, 690, 960, 1258, 1294, 1357, 1878
StyI CCWWGG 5 cut(s) 667, 1677, 1870, 2251, 2351
TaiI ACGT 2 cut(s) 407, 2048
TaqI TCGA 5 cut(s) 468, 814, 1193, 1255, 1306
TaqII GACCGA 1 cut(s) 1805
TatI WGTACW 2 cut(s) 556, 2502
TauI GCSGC 4 cut(s) 28, 799, 2015, 2300
TfiI GAWTC 5 cut(s) 108, 358, 383, 781, 979
Tru1I TTAA 8 cut(s) 75, 255, 272, 462, 479, 1187, 1683, 1698
Tru9I TTAA 8 cut(s) 75, 255, 272, 462, 479, 1187, 1683, 1698
TscAI CASTG 6 cut(s) 105, 118, 1033, 1183, 1830, 2274
TseFI GTSAC 2 cut(s) 790, 2269
Tsp45I GTSAC 2 cut(s) 790, 2269
TspGWI ACGGA 2 cut(s) 366, 1761
TspRI CASTG 6 cut(s) 105, 118, 1033, 1183, 1830, 2274
Tth111I GACNNNGTC 2 cut(s) 547, 1302
Van91I CCANNNNNTGG 2 cut(s) 56, 2204
VpaK11BI GGWCC 1 cut(s) 1006
XapI RAATTY 4 cut(s) 131, 1142, 1415, 1585
XbaI TCTAGA 2 cut(s) 982, 1099
XceI RCATGY 3 cut(s) 32, 1676, 2040
XcmI CCANNNNNNNNNTGG 1 cut(s) 1435
XhoI CTCGAG 1 cut(s) 813
XmiI GTMKAC 2 cut(s) 1377, 1443
XmnI GAANNNNTTC 1 cut(s) 2447
XspI CTAG 7 cut(s) 95, 213, 983, 1100, 1221, 1550, 2126
ZrmI AGTACT 1 cut(s) 558
Zsp2I ATGCAT 1 cut(s) 2038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.